CN101218872A - Isatis root cultivation method - Google Patents
Isatis root cultivation method Download PDFInfo
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- CN101218872A CN101218872A CNA2008100070668A CN200810007066A CN101218872A CN 101218872 A CN101218872 A CN 101218872A CN A2008100070668 A CNA2008100070668 A CN A2008100070668A CN 200810007066 A CN200810007066 A CN 200810007066A CN 101218872 A CN101218872 A CN 101218872A
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- radix isatidis
- cultivation
- cultivation method
- isatidis
- gathering
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01D—HARVESTING; MOWING
- A01D91/00—Methods for harvesting agricultural products
- A01D91/02—Products growing in the soil
Abstract
The invention discloses a method for planting isatis including the following steps:(1) land preparing;(2) seedling culturing: immersing the woad seeds for 11 to 13 hours with a potassium permanganate with a quality chroma of 2 to 4 percent at a temperature of 40 to 50 DEG C, mixing with silver sands or plant ash after fishing out and drying, sowing uniformly; (3) field management; (4) harvesting and processing: the dyers woad leaves are harvested in November of lunar calendar in the same year when the woad is planted; selecting a sunny day, cutting off the part on the ground, collecting and drying; immersing the roots after 7 to 14 days after harvesting the dyers woad leaves, removing rhizomes and clay and drying. Utilizing the planting method of the invention can improve the quality of isatis.
Description
Technical field
The present invention relates to a kind of cultivation method of Radix Isatidis.
Background technology
Radix Isatidis Radix Isatidis is the dry root of cruciferae isatis Isatis indigotica Fort., is clearing heat and detoxicating conventional Chinese medicine, and normal and other Chinese medicine is formed compound, is widely used in multiple disease such as influenza, encephalitis, hepatitis etc.
Woaded blue is biennial vegetation, is distributed in ground such as China Inner Mongol, Shaanxi, Gansu, Hebei, Shandong, Jiangsu, Zhejiang, Anhui, Guizhou, and is normal for cultivating.
The source difference of Radix Isatidis is used as Chinese medicine, also there are differences, and then shows as the difference on the drug effect clinically.This is relevant with concrete production environment and cultivation method, therefore, how to obtain the Radix Isatidis of high-quality, is the target that woaded blue plant personnel and enterprise are pursued.
Summary of the invention
Purpose of the present invention just is to provide a kind of cultivation method of Radix Isatidis, the Radix Isatidis that utilizes this method to obtain, and quality is higher, has better effect aspect the endotoxemia being used to prevent and treat.
The present invention adopts following technical scheme: a kind of cultivation method of Radix Isatidis, and comprise whole ground, grow seedlings, field management and gathering, it is characterized in that:
When growing seedlings, is that 2～4% potassium permanganate were soaked seed 11～13 hours with 40 ℃～50 ℃ mass concentrations earlier with the woaded blue seed, pull out and mix fine sand after drying or ash broadcasts sowing;
When gathering, folium isatidis (leaf of woaded blue) is gathered at woaded blue cultivation lunar calendar then November, and choosing is fine, prescinds acrial part, dries after the collection; Behind the folium isatidis of gathering, uprooted in 7～14 days, remove clean reed head and earth, dry.
Comparative optimization be, to select 3 years or the above husky territory of not planting Radix Isatidis and having irrigation condition during whole ground, plough deeply 25～35 centimetres, use 1500～2000 kilograms of the compost that becomes thoroughly decomposed or barnyard manure for every mu, or every mu apply 100～120 kilograms of humic acid biological fertilizers, furrow are made in thin, the leveling of rake then.
Described field management process can comprise: carry out thinning and fill the gaps with seedlings when 6～10 centimetres of heights of seedling, carry out intertill and clean tillage simultaneously, Feng Hanghou forbids weeding; Arid is in time watered; Should note the ditch cleaning draining rainy season, prevents mashed root of ponding and introduced disease between furrow; Dispenser prevention and elimination of disease and pests in the process of growth; Impose fertilizer 1～3 time.
Radix Isatidis (root of woaded blue) with this method acquisition of the present invention, best in quality, adopt endotoxin to cause rabbit fever models and investigate its correlation effect, show through test, the medicinal extract high and low dose group of using the Radix Isatidis extraction of this method cultivation can significantly suppress time body temperature index of Response TRI
4Rapid rising, reduce the degree (P＜0.05 or P＜0.01) of endotoxin pyrogenic effect, illustrate that this method of employing can improve the antiendotoxin activity intensity of Radix Isatidis, has better quality.
Embodiment
The invention will be further described below in conjunction with specific embodiment, to help understanding content of the present invention.
One, embodiment 1
Cultivation place: White Cloud Mountain, Fuyang Radix Isatidis company of Hutchison China Trade Holdings cultivation base;
(1) choosing ground and whole ground: selected 3 years or the above husky territory of not planting Radix Isatidis and having irrigation condition, the tractor-ploughing in time of preceding crop results back is ploughed deeply about 30 centimetres, use through fully becoming thoroughly decomposed for every mu, meet 1500～2000 kilograms of the compost of sanitary standard or barnyard manure, also can every mu apply " humic acid biological fertilizer " 100～120 kilograms, thin, the leveling of rake is then made wide about 2 meters, 25 centimetres of furrow widths, high 25 centimetres furrow.
(2) grow seedlings: clear and bright to carrying out around grain rains.Before the sowing seed was soaked seed 12 hours with 40 ℃～50 ℃ 3% potassium permanganate, standby to carry out vernalization and sterilization, to pull out drying, mix fine sand or ash and can broadcast sowing.Uniform broadcasting covers 3～5 centimetres with rake with soil in smooth good plot.2 kilograms of every mu of sowing quantities are broadcast the back and can be emerged in 5-10 days.
(3) field management: carry out thinning and fill the gaps with seedlings seeding row spacing during 6～10 centimetres of heights of seedling: be about 20 centimetres of 5～7 cm x.Look careless growing state is in time intertilled, weeding, Feng Hanghou forbids weeding.Impose fertilizer in vegetative period 1～3 time, 25 kilograms every mu of humic acid biological fertilizers evenly apply in the middle of the line-spacing, plough under in the soil during intertillage.Arid is in time watered, can be before at 10 in the morning, pour water after at 4 in afternoon, if water under the exposure in sunshine at noon, and the blade of easily burning.Should note the ditch cleaning draining rainy season, prevents mashed root of ponding and introduced disease between furrow.
Vegetative period the extermination of disease and insect pest in the following way:
1., downy mildew: the main harm blade, also can endanger petiole.The their early stage vacuum side of blade produces white or canescence mustiness thing, and the very not tangible yellow-white scab in border takes place on the blade face.The flavescence gradually of morbidity rear blade, it is withered and dead to be brown at last.Their early stage is sprayed with 1: 1: 200 bordeaux mixture, or sprays with 600 times of liquid of 60% zineb or 500 times of liquid of 50% carbendazim.Spray in per 7 days 1 time connects and sprays 2-3 time.
2., stalk break: this disease can endanger complete stool, by soil infection.Morbidity season spray medicine protection: use grey sulphur mixture in the plant base portion; Their early stage sprayed once continuous 2～3 times with 500～600 times of sprayings of 65% zineb every 7 days.Or with 50% carbendazim, 500～1000 times of liquid of topsin, soup is concentrated and is sprayed the plant middle and lower part, generally every spray in 7～10 days once.
3., root rot: period of disease is watered the diseased plant root with 800～1000 times of liquid of 50% topsin.
4., white blister: their early stage sprays bordeaux mixture (1: 1: 120), every 7 days 1 time, sprays continuously 2～3 times.
5., black spot: their early stage, spray bordeaux mixture (1: 1: 120) or 600～800 times of liquid of 65% zineb, every spray in 10～15 days 1 time, every mu of consumption 75kg sprays 2～3 times continuously.500 times of liquid of the also available 50% metalaxyl wetting powder of their early stage spray.
6., imported cabbageworm (cabbage caterpillar): endanger blade 5～June.The method of control is that instar larvae is sprayed every mu of consumption 20-30 gram with cabbage caterpillar particle virus Bt composite insecticide; Or with 800～1000 times of liquid sprayings of bacillus thuringiensis wetting powder or particle; Or with 1000 times of liquid sprayings of 90% chlorophos crystal or flolimat control.Diamond-back moth: the same cabbage caterpillar of the method for preventing and treating.
7., three-spotted plusia: every mu sprays 2～2.5kg, 2.5% chlorophos pulvis.
8., aphid: the tender stem of main harm, leaf and the bud of just having extracted out.With 1500～2000 times of liquid sprayings of 40% dimethoate emulsifiable concentrate.
(4), gather and process:
1, gathering of folium isatidis: gather in November, first.Choosing is fine, prescinds acrial part, collects to claim to dry behind the fresh weight.
2, gathering of root: behind the folium isatidis of gathering, uprooted in 7～14 days, remove clean reed head and earth, dry.
Two, Comparative Examples:
In order to verify whether cultivation method of the present invention influences the quality of the Radix Isatidis product of acquisition (root of woaded blue), carried out the contrast cultivation in same plot, adopted traditional cultivation method, be included in before the breeding with 40 ℃～50 ℃ emerge in worm water seed 12 hours with the difference of embodiment 1, gathering of root is to gather simultaneously with folium isatidis.
Three, contrast experiment's example
For the quality of scientific evaluation Radix Isatidis, in conjunction with its product clinical indication, consider drug activity judging quota in the body emphatically, adopt endotoxin to cause rabbit fever models and investigate its correlation effect.
1. material and instrument
1.1 medicine
Radix Isatidis extractum A: adopt Radix Isatidis (root of the woaded blue) medicinal material that cultivation method obtains among the embodiment 1, press the preparation of banlangen granules, banlangen keli extraction process, provide by Guangzhou Baiyunshan Heji Huangpu Chinese Medicine Co., Ltd..
Radix Isatidis extractum B: adopt the Radix Isatidis (root of woaded blue) of cultivation method (conventional method) acquisition of Comparative Examples, press the preparation of banlangen granules, banlangen keli extraction process, provide by Guangzhou Baiyunshan Heji Huangpu Chinese Medicine Co., Ltd..
Radix Isatidis extractum C: press the preparation of banlangen granules, banlangen keli extraction process, portion provides by the HuaZhong Science University, TongJi medical school, TongJi Hospital pharmacy.
Aspirin enteric coated tablet: Hebei Shineway Pharmaceutical Co., Ltd, lot number 0704121.It is an amount of to get Radix Isatidis extractum A, B, adds an amount of distilled water respectively, and mixing is made and is equivalent to that crude drug in whole is 2.0,1.0, the soup of 0.5g/mL; Radix Isatidis extractum C makes with method and is equivalent to the soup that crude drug in whole is 1.0g/mL, and is standby.
1.2 animal
Japan large ear rabbit, male and female are regardless of, body weight 1.8-2.5kg, culturing Co., Ltd by the multifarious good standing grain laboratory animal in Wuhan City provides, the animal quality certification number: SCXK (Hubei Province) 2007-0006.
The experiment place is provided by Tongji Medical College, Huazhong Science and Technology Univ.'s Experimental Animal Center.
1.3 reagent
Lipopolysaccharides (LPS, 5mg, Sigma company, L-3755); It is pure that all the other reagent are analysis.
2. method and result
Select healthy rabbits, survey the anus temperature every day 2 times, 3d progressively adapts to it continuously; Body temperature should be in 38.0～39.6 ℃, and the body temperature fluctuation is no more than 0.3 ℃.Measure the result in advance according to animal heat, filter out 72 of the qualified rabbit of body temperature, be divided into 9 groups at random, 8 every group.The high, medium and low dosage group of medicine respectively by 6.0,3.0,1.5gkg
-1Irritate stomach and give medicine, model group gives with volume distilled water, and positive controls is with 200mgkg
-1Gavage aspirin.Except that positive controls, all the other respectively organize administration every day 1 time, continuously 5d.1.5h after the last administration measures the rabbit basal body temperature, totally 3 times, gets its average.Behind the 1h, auricular vein injection LPS 200ngkg
-1, measure rabbit body temperature in endotoxin attack back 0.5,1,2,3,6h, observe its situation of change, and compare with basal body temperature, calculate maximum temperature difference (Δ T), time body temperature index of Response (TRI
4, try to achieve area under the temperature time curve with trapezoidal method), with TRI
4For suppressing percentage according to calculating heating.
Data are handled: the factor that influences the endotoxin pyrogenic reaction experiment is numerous, mainly comprise laboratory animal general state (body weight, spirit, appetite, excreta, active situation etc.), experimental situation (temperature, humidity) etc., especially the rabbit basal body temperature should remain in 38.0～39.6 ℃ of scopes, and the highest and minimum mensuration body temperature differs and is no more than 0.4 ℃ of person and can includes basis in.Because in the experiment detailed process, exist the part animal because of general state, room temperature change, disturbance excessively, the influences such as action interference of noise and experiment operator, cause its temperature deviation to some extent, these type of data are all disallowable.On initial data (n=8) basis, reject some body temperature and do not meet relevant data and the animal individual (rejecting 2 data for every group) of including standard code in,
Medicine group and model group are carried out the t check relatively, the results are shown in Table 1.
The influence of table 1 pair endotaxin induction fever in rabbits effect
| Group | Dosage (g/kg) | Number of animals | ΔT(℃) | TRI 4 | Inhibiting rate |
| Dosage among the dosage Radix Isatidis B low dosage Radix Isatidis C among the dosage Radix Isatidis A low dosage Radix Isatidis B high dose Radix Isatidis B among the model group aspirin group Radix Isatidis A high dose Radix Isatidis A | 0.2 6.0 3.0 1.5 6.0 3.0 1.5 3.0 | 6 6 6 6 6 6 6 6 6 | 1.14±0.30 1.07±0.36 1.02±0.33 1.06±0.42 0.87±0.34 1.05±0.23 1.13±0.47 1.28±0.27 1.05±0.39 | 3.67±1.20 2.51±1.44 1.06±0.84 ** 2.55±1.42 1.88±1.57 * 2.85±1.56 3.39±1.12 4.21±0.93 3.12±1.05 | / 31.64％ 71.06％ 30.58％ 48.90％ 22.31％ 7.65％ -14.72％ 15.06％ |
Annotate: compare with model group
*P＜0.05
*P＜0.01
By table 1 result, compare with model group, Radix Isatidis A medicinal extract high and low dose group can significantly suppress time body temperature index of Response TRI
4Rapid rising, reduce the degree (P＜0.05 or P＜0.01) of endotoxin pyrogenic effect.But each group is to the reduction degree of maximum temperature difference Δ T and model group unknown significance difference relatively, shows that its intensity to the fever in rabbits effect of endotaxin induction do not have significance and influence.From the heating inhibiting rate, Radix Isatidis B medicinal extract presents dose-dependent inhibition endotoxin heating effect, and Radix Isatidis A medicinal extract does not have significant correlation, and suppressing activity intensity is Radix Isatidis A＞Radix Isatidis C＞Radix Isatidis B.
Conclusion: (1) Radix Isatidis A medicinal extract can significantly suppress the fever in rabbits effect of endotaxin induction, and its activity intensity is higher relatively; (2) Radix Isatidis is for the degree (TRI of endotaxin induction fever in rabbits effect
4) have certain antagonistic effect, but for its calorific intensity (Δ T) control ability slightly a little less than; (3) from the antiendotoxin effect analysis, there is some difference between the Radix Isatidis different batches product of White Cloud Mountain, but its quality product (Radix Isatidis A) effect intensity is significantly higher than the market like product.That is, adopt method cultivation Radix Isatidis of the present invention, quality is better, should include GAP cultivation standard in, carries out the industrialization production of Radix Isatidis.
Claims (4)
1. the cultivation method of a Radix Isatidis comprises whole ground, grows seedlings, field management and gathering, and it is characterized in that:
When growing seedlings, is that 2～4% potassium permanganate were soaked seed 11～13 hours with 40 ℃～50 ℃ mass concentrations earlier with the woaded blue seed, pull out and mix fine sand after drying or ash broadcasts sowing;
When gathering, folium isatidis is gathered at woaded blue cultivation lunar calendar then November, and choosing is fine, prescinds acrial part, receives and dries; Behind the folium isatidis of gathering, uprooted in 7～14 days, remove clean reed head and earth, dry.
2. the cultivation method of Radix Isatidis as claimed in claim 1, it is characterized in that described whole ground process is: selected 3 years or above husky territory of not planting Radix Isatidis, plough deeply 25～35 centimetres, use 1500～2000 kilograms of the compost that becomes thoroughly decomposed or barnyard manure for every mu, or every mu apply 100～120 kilograms of humic acid biological fertilizers, furrow are made in thin, the leveling of rake then.
3. the cultivation method of Radix Isatidis as claimed in claim 1 is characterized in that described field management process comprises:
Carry out thinning and fill the gaps with seedlings when 6～10 centimetres of heights of seedling, carry out intertill and clean tillage simultaneously, Feng Hanghou forbids weeding;
Arid is in time watered, and should note the ditch cleaning draining rainy season, prevents mashed root of ponding and introduced disease between furrow;
Dispenser prevention and elimination of disease and pests in the process of growth;
Impose fertilizer 1～3 time.
4. the cultivation breeding method of Radix Isatidis as claimed in claim 3 is characterized in that: the described process of watering is to carry out before at 10 in the morning, after at 4 in afternoon.
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| CNA2008100070668A CN101218872A (en) | 2008-02-01 | 2008-02-01 | Isatis root cultivation method |
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| CNA2008100070668A CN101218872A (en) | 2008-02-01 | 2008-02-01 | Isatis root cultivation method |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102040416A (en) * | 2010-11-19 | 2011-05-04 | 黑龙江八一农垦大学 | Preparation and application methods of special fertilizer for continuous-cropping isatis indigotica |
| CN102696382A (en) * | 2012-06-15 | 2012-10-03 | 山西省农业科学院经济作物研究所 | Indigowoad root suitable for sprout production and method for growing indigowoad root sprouts |
| CN102763544A (en) * | 2012-06-30 | 2012-11-07 | 芜湖金凤中草药标本科技有限公司 | Low-cost method for planting Dyer's woad in sandy lands |
| CN102763541A (en) * | 2012-06-30 | 2012-11-07 | 芜湖金凤中草药标本科技有限公司 | Method for planting isatis roots on sand soil land parcels |
| CN102763538A (en) * | 2012-06-30 | 2012-11-07 | 芜湖金凤中草药标本科技有限公司 | Method for planting Dyer's woad in clay plots |
| CN102763542A (en) * | 2012-06-30 | 2012-11-07 | 芜湖金凤中草药标本科技有限公司 | Method for planting Dyer's woad in loam lands |
| CN104488484A (en) * | 2014-11-18 | 2015-04-08 | 师宗广朋农业科技有限公司 | Pollution-free cultivation method for isatis root |
| CN105027912A (en) * | 2015-07-20 | 2015-11-11 | 江苏农牧科技职业学院 | Cultivation method for interplanting officinal cape jasmines and radix isatidis |
| CN105123174A (en) * | 2015-07-22 | 2015-12-09 | 莫玉明 | Cultivation method for baphicanthus cusia bremek |
| CN105474963A (en) * | 2015-12-15 | 2016-04-13 | 安徽省康君食品有限公司 | Radix Salviae Miltiorrhizae planting method |
| CN106612704A (en) * | 2015-11-03 | 2017-05-10 | 安国市聚药堂药业有限公司 | Pre-treatment method of isatis root culture |
| CN107432173A (en) * | 2017-07-31 | 2017-12-05 | 贵州文昌蓝靛有限公司 | A kind of implantation methods of indigo grass |
| CN108094062A (en) * | 2017-12-07 | 2018-06-01 | 安徽林昊农业发展有限公司 | A kind of implantation methods of Chinese herbal medicine |
| CN109769677A (en) * | 2019-03-22 | 2019-05-21 | 福建省中科生物股份有限公司 | A kind of plant factor's Radix Isatidis ciltivating process |
| CN113331202A (en) * | 2021-05-06 | 2021-09-03 | 中国中药有限公司 | Isatis root seed coating agent, coating method and application thereof |
| CN114424733A (en) * | 2022-01-27 | 2022-05-03 | 黑龙江橘香本草科技有限公司 | Artificial planting method of isatis roots |
-
2008
- 2008-02-01 CN CNA2008100070668A patent/CN101218872A/en active Pending
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102040416A (en) * | 2010-11-19 | 2011-05-04 | 黑龙江八一农垦大学 | Preparation and application methods of special fertilizer for continuous-cropping isatis indigotica |
| CN102040416B (en) * | 2010-11-19 | 2013-01-02 | 黑龙江八一农垦大学 | Preparation and application methods of special fertilizer for continuous-cropping isatis indigotica |
| CN102696382A (en) * | 2012-06-15 | 2012-10-03 | 山西省农业科学院经济作物研究所 | Indigowoad root suitable for sprout production and method for growing indigowoad root sprouts |
| CN102763544A (en) * | 2012-06-30 | 2012-11-07 | 芜湖金凤中草药标本科技有限公司 | Low-cost method for planting Dyer's woad in sandy lands |
| CN102763541A (en) * | 2012-06-30 | 2012-11-07 | 芜湖金凤中草药标本科技有限公司 | Method for planting isatis roots on sand soil land parcels |
| CN102763538A (en) * | 2012-06-30 | 2012-11-07 | 芜湖金凤中草药标本科技有限公司 | Method for planting Dyer's woad in clay plots |
| CN102763542A (en) * | 2012-06-30 | 2012-11-07 | 芜湖金凤中草药标本科技有限公司 | Method for planting Dyer's woad in loam lands |
| CN102763544B (en) * | 2012-06-30 | 2014-03-19 | 芜湖金凤中草药标本科技有限公司 | Method for planting Dyer's woad in sandy lands |
| CN102763541B (en) * | 2012-06-30 | 2014-05-07 | 芜湖金凤中草药标本科技有限公司 | Method for planting isatis roots on sand soil land parcels |
| CN102763538B (en) * | 2012-06-30 | 2014-05-07 | 芜湖金凤中草药标本科技有限公司 | Method for planting Dyer's woad in clay plots |
| CN104488484A (en) * | 2014-11-18 | 2015-04-08 | 师宗广朋农业科技有限公司 | Pollution-free cultivation method for isatis root |
| CN105027912A (en) * | 2015-07-20 | 2015-11-11 | 江苏农牧科技职业学院 | Cultivation method for interplanting officinal cape jasmines and radix isatidis |
| CN105123174A (en) * | 2015-07-22 | 2015-12-09 | 莫玉明 | Cultivation method for baphicanthus cusia bremek |
| CN106612704A (en) * | 2015-11-03 | 2017-05-10 | 安国市聚药堂药业有限公司 | Pre-treatment method of isatis root culture |
| CN105474963A (en) * | 2015-12-15 | 2016-04-13 | 安徽省康君食品有限公司 | Radix Salviae Miltiorrhizae planting method |
| CN107432173A (en) * | 2017-07-31 | 2017-12-05 | 贵州文昌蓝靛有限公司 | A kind of implantation methods of indigo grass |
| CN108094062A (en) * | 2017-12-07 | 2018-06-01 | 安徽林昊农业发展有限公司 | A kind of implantation methods of Chinese herbal medicine |
| CN109769677A (en) * | 2019-03-22 | 2019-05-21 | 福建省中科生物股份有限公司 | A kind of plant factor's Radix Isatidis ciltivating process |
| CN113331202A (en) * | 2021-05-06 | 2021-09-03 | 中国中药有限公司 | Isatis root seed coating agent, coating method and application thereof |
| CN114424733A (en) * | 2022-01-27 | 2022-05-03 | 黑龙江橘香本草科技有限公司 | Artificial planting method of isatis roots |
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