CN107371960A - A kind of implantation methods of fertile mandarin orange - Google Patents

A kind of implantation methods of fertile mandarin orange Download PDF

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Publication number
CN107371960A
CN107371960A CN201710658421.7A CN201710658421A CN107371960A CN 107371960 A CN107371960 A CN 107371960A CN 201710658421 A CN201710658421 A CN 201710658421A CN 107371960 A CN107371960 A CN 107371960A
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parts
weight
bacterium solution
fruit
mandarin orange
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黄自宇
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Guangxi Chisheng Agricultural Technology Co Ltd
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Guangxi Chisheng Agricultural Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005—Cultivation methods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C21/00—Methods of fertilising, sowing or planting
    • A01C21/005—Following a specific plan, e.g. pattern
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05C—NITROGENOUS FERTILISERS
    • C05C11/00—Other nitrogenous fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Botany (AREA)
  • Pest Control & Pesticides (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention specifically includes following steps, (1) seedling process：Select disease-free, root system completely to irrigate the strain of mandarin orange seedling as seedling, root system is soaked into the 5S of taking root liquid 3 before planting；(2) soil cultivation；(3) plant；(4) water and fertilizer management；(5) trimming leaf and shield flower and fruit：After fruit picking and before annual rudiment in 2 April, cut off and intersect branch, weak branch, sick branch and deadwood；The long shoot of summer sprouting is cut off, removes diseased plant in time；(6) fruit picking and manage after adopting.It is an advantage of the invention that this method is simple and easy, survival rate, fruit bearing rate can be improved, pest and disease damage Probability is reduced, the amount of amount and sugar of the increase fruit containing selenium element, improves the quality of fruit, there is higher economic value.

Description

A kind of implantation methods of fertile mandarin orange
【Technical field】
The present invention relates to agricultural technology field, and in particular to a kind of implantation methods of fertile mandarin orange.
【Background technology】
Fertile mandarin orange is the cenospecies of " temple " tangerine orange and " Dan Xi " red tangerine.The kind belongs to late-maturing Citrus after Cross Pollination kind.It is raw Growing way is strong, and tree crown initial stage is in nature globular model, as a result after progressively open a business, have short thorn on branch.Not only taste is fragrant and sweet for fertile mandarin orange, also has There is very high nutritive value, it contains soluble solid 13.3%, titratable acid 0.58%, the milli of inverted sugar 12.76 grams/100 Rise, 6.84 grams/100 milliliters of reduced sugar, 23.69 milligrams/100 milliliters of Vc contents, Gu sour ratio 22.9.Edible rate 74.62%, juice Rate 59.56%.
The maturity period of traditional citrus is or so annual December, and traditional citrus is carried out into breed improvement and complete sets of Techniques Afterwards, fructescence is deferred to the citrus after 2 months next years and just belongs to late-maturing citrus.This fertile mandarin orange and common late-maturing citrus compared with Come, it is even more that can extend to or so June next year to hang the tree phase, is " late-maturing king " in typical citrus.Moreover, this mandarin orange The sugar content of tangerine is also significantly larger than common citrus, has evening in maturity period, that the collecting period is long, tasty mouthfeel is tasty and refreshing, peels easily etc. is all More advantages.With expanding economy, people also gradually strengthen the mouthfeel of fruit and the pursuit of health degree, in these areas excellent Point causes the market demand of fertile mandarin orange increasingly to double, therefore fertile mandarin orange industry has wide market prospects and economic benefit at home.
At present, when to fertile mandarin orange applying fruit trees with fertilizer, also there is method not science and fertilising position improperly phenomenon.It is most of People's fertilising position is nearer from trunk, not only reduces the utilization rate of fertilizer, and has injured fertile mandarin orange seedling main root, influences fertile mandarin orange seedling Take root, yield positive results, cause the underproduction.In a word, also include the problem of the fertile mandarin orange of plantation is present at this stage：The fertile mandarin orange soil of plantation is chosen Unreasonable, unbalanced using Fertilizer nutrient, planting process manages not science, irrigates the problems such as mandarin orange is slow-growing, fruit is small, causes to plant The production time of the fertile mandarin orange of plant is long and yield is not high.
【The content of the invention】
The goal of the invention of the present invention is：For it is above-mentioned the problem of, the present invention provides a kind of implantation methods of fertile mandarin orange, the party Method is simple and easy, can improve survival rate, fruit bearing rate, reduces pest and disease damage Probability, amount and sugar of the increase fruit containing selenium element Amount, improves the quality of fruit, has higher economic value.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of implantation methods of fertile mandarin orange, specifically include following steps,
(1) seedling process：Select disease-free, root system completely to irrigate the strain of mandarin orange seedling as seedling, root system is soaked given birth to before planting Root liquid 3-5S；
(2) soil cultivation：It is 20 ° of mountain region to select the physical features gradient, and the depth turned over is 65-70cm, then is raked the soil level to Horizon It is whole, and dig gutter；
(3) plant：Implantation time is in the 9-10 months, spacing in the rows 3-3.5*2.5-3m, digs cave, the specification in cave is wide 15cm, height 15cm, it will soak in planting seedlings and cave after taking root liquid when field planting, then earthed up, the graft union of seedling is exposed ground Face 3-5cm, ridging is a base diameter 70-80cm, is highly 18-20cm hill, and pours root water in time, then The fine sand of a thickness 2-3cm is sprinkled on the surface of hill；
(4) water and fertilizer management：When crown height is to 0.9m, every plant of application organic fertilizer 3-5kg is annual after the Second Year of field planting Autumn centered on tree crown, using 2-3m as diameter ditching, apply 10-15kg every plant of organic fertilizer；
The organic fertilizer is that following raw material is prepared：1-5 parts by weight coprinus comatus leftover bits and pieces, 0.3-0.75 parts by weight mulberries Strangle careless, 0.35-0.75 parts by weight folium cortex eucommiae, 0.3-0.75 parts by weight selenocystein, 0.15-0.55 parts by weight of soybean shell, 0.85-1.75 parts by weight garlic, 0.5-1.35 parts by weight oriental sesame pericarp, 0.35-0.95 parts by weight fructus cannabis, 0.25-0.85 weight Part silkworm chrysalis, the compound beneficial bacterium of 0.1-0.5 parts by weight, 0.1-0.25 parts by weight honey, 0.25-0.55 parts by weight of lemon, 0.35- 0.65 parts by weight indigo plant plum；
(5) trimming leaf and shield flower and fruit：After fruit picking and before annual 2-4 months rudiment, cut off and intersect branch, weak branch, disease Worm branch and deadwood；The long shoot of summer sprouting is cut off, removes diseased plant in time；
(6) fruit picking and manage after adopting：Harvested in time after fruit maturation, it is shallow to planting site progress every 1 year during plantation Turn over, and improved the soil with quick lime.
Preferably, the organic fertilizer is that following raw material is prepared：1.5-4 parts by weight coprinus comatus leftover bits and pieces, 0.4-0.6 Parts by weight mulberry strangle careless, 0.5-0.6 parts by weight folium cortex eucommiae, 0.4-0.6 parts by weight selenocystein, 0.2-0.6 parts by weight of soybean shell, 0.95-1.5 parts by weight garlic, 0.7-1.2 parts by weight oriental sesame pericarp, 0.5-0.8 parts by weight fructus cannabis, 0.3-0.7 parts by weight silkworm chrysalis, The compound beneficial bacterium of 0.2-0.4 parts by weight, 0.15-0.2 parts by weight honey, 0.3-0.5 parts by weight of lemon, 0.4-0.6 parts by weight are blue Plum.
It is prepared it is further preferred that the organic fertilizer is following raw material：2.8 parts by weight coprinus comatus leftover bits and pieces, 0.5 weight It is big to measure part Sang Lecao, 0.55 parts by weight folium cortex eucommiae, 0.5 parts by weight selenocystein, 0.4 parts by weight of soybean shell, 1.2 parts by weight Garlic, 0.9 parts by weight oriental sesame pericarp, 0.6 parts by weight fructus cannabis, 0.5 parts by weight silkworm chrysalis, the compound beneficial bacterium of 0.3 parts by weight, 0.18 weight Part honey, 0.4 parts by weight of lemon, 0.5 parts by weight indigo plant plum.
Further illustrate, the raw material that the compound beneficial bacterium includes following parts by weight is prepared into：Lactic acid bacterial liquid 0.1-0.3 parts, Dipel bacterium solution 0.15-0.25 parts, Lactobacillus plantarum bacterium solution 0.31-0.45 parts, yellow mould bacterium solution 0.10- 0.20 part, lactobacillus bulgaricus bacterium solution 0.20-0.35 parts, bacillus subtilis bacterium solution 0.15-0.30 parts.
Preferably, raw material of the compound beneficial bacterium including following parts by weight is prepared into：0.25 part of lactic acid bacterial liquid, Soviet Union 0.2 part of cloud gold bacillus bacterium solution, 0.36 part of Lactobacillus plantarum bacterium solution, 0.15 part of yellow mould bacterium solution, lactobacillus bulgaricus bacterium solution 0.28 part, 0.25 part of bacillus subtilis bacterium solution.
Further illustrate, effective bacteria containing amount of the lactic acid bacterial liquid in the compound beneficial bacterium is 3.8 × 108-1.9×109 Individual/mL, effective bacteria containing amount of Dipel bacterium solution are 3.25 × 108-4.16×108Individual/mL, Lactobacillus plantarum bacterium solution have It is 2.98 × 10 to imitate bacteria containing amount8-3.26×109Individual/mL, effective bacteria containing amount of yellow mould bacterium solution are 1.65 × 109-2.58×109 Individual/mL, effective bacteria containing amount of lactobacillus bulgaricus bacterium solution are 2.89 × 108-3.69×109Individual/mL, bacillus subtilis bacterium Effective bacteria containing amount of liquid is 2.56 × 108-3.42×109Individual/mL.
In the present invention, further illustrate, the taking root liquid is that root-inducing powder is watered into concentration is 10%wt-20%wt； Also added with 0.55%wt-0.85%wt mono-nitration guaiacol sodium, 75%wt-85%wt ethanol, 0.25%wt-0.32% Wt methyl α-naphthyl acetates, and the volume ratio of the taking root liquid of addition, mono-nitration guaiacol sodium, ethanol, methyl α-naphthyl acetate is 10:1-2:1.5-2.5: 1-2。
Further illustrating, the Sang Lecao is extracted 10 minutes by chopping with water, filtering, altogether extraction 2-3 times, then Reach the extract solution of 50%-65% linoleic acid compositions to content.
Further illustrate, the folium cortex eucommiae crushes after being dried, by circulating ultrasonic extraction element according to comminuted powder with Solvent for extraction is 1 according to volume ratio:2 ratio mixing extraction, 50-55 degrees Celsius of Extracting temperature, supersonic frequency 50- 60KHz, extract 2-5 minutes, circulation extraction 5 times merges 5 liquid after filtering, liquid loads solvent recovery enrichment facility 50-60 Degree Celsius recycling design, then pressure be 10-15MPa, temperature is 30-35 DEG C, CO2Flow 15kg/h, the time be 15 minutes, Refined filtration, bark of eucommia leaf extract, the composition in extract contain linoleic acid 59.3%, leukotrienes 3.6%.
Further illustrate, the fructus cannabis volatilizes by broken, soxhlet extraction degreasing, water extract-alcohol precipitation, centrifugation, water-bath, be cold It is lyophilized dry, produce purple common perilla seed, main component be linoleic acid (ω -6 systems)/alpha-linolenic acid (ω -3 systems) ratio be 69%/ 12%.
Further illustrate, the lemon adds water and cellulase, volume ratio 1 after carrying out peeling remove seed:1:0.02- 0.05, digested, then after carrying out enzyme deactivation, obtain enzymolysis liquid, the pH of enzymolysis liquid is adjusted to 4-6, uses ethyl acetate Extraction, takes organic phase, and the 1-3wt.% of weight such as addition sodium hydrate aqueous solution, stirs, extracted in organic phase Take, phase of fetching water, aqueous phase feeding milipore filter is filtered, then the penetrating fluid of milipore filter is concentrated with NF membrane, then carry out After drying, lemon chromocor extract is obtained.
Further illustrate, the blue plum, which is added in ethanol solution, carries out refluxing extraction 2-5 times, and then reclaiming concentrate is carried out Centrifuge, take supernatant, then add diatomite mixing and carry out press filtration, by filtrate by being mounted with the tree of poly (ether sulfone) film material Fat is filtered, filter molecular cut off 5KD, then progressive wash, obtain eluent, eluent is carried out being concentrated into volume be 1.12 times can obtain blueberry chromocor extract.
The preparation method of the organic fertilizer of the application is：By coprinus comatus leftover bits and pieces, garlic, soybean peel, oriental sesame pericarp, silkworm chrysalis, honeybee Honey obtains basic fertilizer after building heap and fermenting 8-10 days, continues addition Sang Lecao extract solutions, eucommia leaf extract, lemon flavones and carries Take thing, blue plum chromocor extract, selenocystein to be mixed with basic fertilizer, continue to build heap fermentation 3-5 days.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
The implantation methods of the present invention, it is every 100 grams of 15.35-21.34 milligrams that can be obtained in step of applying fertilizer rich in selenium amount Organic fertilizer carry out fertilizing and planting, by internal transport transmission, fertile mandarin orange can fully absorb selenium element in growth period, reach The effect of selenium-rich, substantial amounts of selenium element is rich in the organic fertilizer of the application, and passes through secondary fermentation, can not only ferment release more More micro- organic matters, amino acid, linoleic acid, leukotrienes, the Huang in fertilizer can also be added by the processing of several raw materials Letones content, by plant absorption, the content of the various organic matters in fertile mandarin orange is improved, improves the fruit quality for irrigating mandarin orange, and Compound beneficial bacterium can not only promote fermentation also to have antibacterial action, and combination allicin effect can prevent from planting seedling on mountain Situation with gnawing is dug by mouse, improves the survival rate of field planting, peasant's cost is reduced and increases economic efficiency.
Secondly, present invention take advantage of that multiple kinds of crops discarded object, turns waste into wealth, agricultural waste, protecting ecology balance are reduced.
【Embodiment】
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below Embodiment is closed, the present invention is expanded on further.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, It is not intended to limit the present invention.
Embodiment 1：
A kind of implantation methods of fertile mandarin orange, specifically include following steps,
(1) seedling process：Select disease-free, root system completely to irrigate the strain of mandarin orange seedling as seedling, root system is soaked given birth to before planting Root liquid 3S；
Wherein taking root liquid is that root-inducing powder is watered into concentration is 10%wt；Also it is added with 0.55%wt mono-nitration guaiacol Sodium, 75%wt ethanol, 0.25%wt methyl α-naphthyl acetates, and the taking root liquid of addition, mono-nitration guaiacol sodium, ethanol, methyl α-naphthyl acetate Volume ratio is 10:1：1.5:1；
(2) soil cultivation：Select the physical features gradient be 20 ° of mountain region, the depth turned over is 65cm, then rake the soil level it is smooth to ground, And dig gutter；
(3) plant：Implantation time is in September, spacing in the rows 3*2.5m, digs cave, and the specification in cave is wide 15cm, high 15cm, field planting When will soak in planting seedlings and cave after taking root liquid, then earthed up, the graft union of seedling basseted 3cm, training Soil is a base diameter 70cm, is highly 18cm hill, and pours root water in time, is then spread on the surface of hill Last layer thickness 2cm fine sand；
(4) water and fertilizer management：When crown height is to 0.9m, every plant of application organic fertilizer 3kg is annual after the Second Year of field planting Autumn centered on tree crown, using 2m as diameter ditching, applies 10kg every plant of organic fertilizer；
The organic fertilizer is that following raw material is prepared：1 parts by weight coprinus comatus leftover bits and pieces, 0.3 parts by weight mulberry strangle careless, 0.35 parts by weight folium cortex eucommiae, 0.3 parts by weight selenocystein, 0.15 parts by weight of soybean shell, 0.85 parts by weight garlic, 0.5 weight Part oriental sesame pericarp, 0.35 parts by weight fructus cannabis, 0.25 parts by weight silkworm chrysalis, the compound beneficial bacterium of 0.1 parts by weight, 0.1 parts by weight honey, 0.25 parts by weight of lemon, 0.35 parts by weight indigo plant plum；
The raw material that the compound beneficial bacterium includes following parts by weight is prepared into：0.1 part of lactic acid bacterial liquid, Dipel It is 0.15 part of bacterium solution, 0.31 part of Lactobacillus plantarum bacterium solution, 0.10 part of yellow mould bacterium solution, 0.20 part of lactobacillus bulgaricus bacterium solution, withered Careless 0.15 part of bacillus bacterium solution.
Further illustrate, effective bacteria containing amount of the lactic acid bacterial liquid in the compound beneficial bacterium is 3.8 × 108Individual/mL, Soviet Union Effective bacteria containing amount of cloud gold bacillus bacterium solution is 3.25 × 108Individual/mL, effective bacteria containing amount of Lactobacillus plantarum bacterium solution are 2.98 × 108 Individual/mL, effective bacteria containing amount of yellow mould bacterium solution are 1.65 × 109Individual/mL, effective bacteria containing amount of lactobacillus bulgaricus bacterium solution are 2.89×108Individual/mL, effective bacteria containing amount of bacillus subtilis bacterium solution are 2.56 × 108Individual/mL；
(5) trimming leaf and shield flower and fruit：After fruit picking and before annual rudiment in 2 months, cut off and intersect branch, weak branch, disease pest Branch and deadwood；The long shoot of summer sprouting is cut off, removes diseased plant in time；
(6) fruit picking and manage after adopting：Harvested in time after fruit maturation, it is shallow to planting site progress every 1 year during plantation Turn over, and improved the soil with quick lime.
The preparation method of the organic fertilizer of the application is：
Sang Lecao is extracted 10 minutes by chopping with water, filtering, is extracted 2 times altogether, is then obtained content and is reached 50% Asia The extract solution of oleic acid content；
Folium cortex eucommiae crushes after being dried, by circulating ultrasonic extraction element according to comminuted powder and solvent for extraction according to body Product is than being 1:2 ratio mixing extraction, 50 degrees Celsius, supersonic frequency 50KHz of Extracting temperature, is extracted 2 minutes, circulation extraction 5 It is secondary, 5 liquid are merged after filtering, liquid loads solvent recovery 50 degrees Celsius of recycling designs of enrichment facility, is then in pressure 10MPa, temperature are 30 DEG C, CO2Flow 15kg/h, time are 15 minutes, refined filtration, bark of eucommia leaf extract, in extract into Divide containing linoleic acid 59.3%, leukotrienes 3.6%；
Fructus cannabis is volatilized, is freeze-dried by broken, soxhlet extraction degreasing, water extract-alcohol precipitation, centrifugation, water-bath, produces purple perilla Seed extract, main component are that linoleic acid (ω -6 systems)/alpha-linolenic acid (ω -3 systems) ratio is 69%/12%.
Further illustrate, the lemon adds water and cellulase, volume ratio 1 after carrying out peeling remove seed:1:0.02, carry out Enzymolysis, then after carrying out enzyme deactivation, obtain enzymolysis liquid, the pH of enzymolysis liquid is adjusted to after 4, is extracted with ethyl acetate, take organic Phase, the 1wt.% of weight such as addition sodium hydrate aqueous solution, stirs, is extracted in organic phase, phase of fetching water, by water Mutually it is sent into milipore filter to be filtered, then the penetrating fluid of milipore filter is concentrated with NF membrane, then after being dried, obtains lemon Lemon chromocor extract；
Lan Mei, which is added in ethanol solution, carries out refluxing extraction 2 times, and then reclaiming concentrate is centrifuged, and takes supernatant Liquid, then add diatomite mixing and carry out press filtration, filtrate is filtered by being mounted with the resin of poly (ether sulfone) film material, filter is cut Molecular weight 5KD is stayed, then progressive is washed, and obtains eluent, and it is 1.12 times i.e. available that eluent, which is carried out being concentrated into volume, Blueberry chromocor extract；
Then coprinus comatus leftover bits and pieces, garlic, soybean peel, oriental sesame pericarp, silkworm chrysalis, honey are obtained after building heap and fermenting 8 days Basic fertilizer, continue to add Sang Lecao extract solutions, eucommia leaf extract, lemon chromocor extract, blue plum chromocor extract, the Guang of selenium half Propylhomoserin is mixed with basic fertilizer, continues to build heap fermentation 3 days.
Embodiment 2：
A kind of implantation methods of fertile mandarin orange, specifically include following steps,
(1) seedling process：Select disease-free, root system completely to irrigate the strain of mandarin orange seedling as seedling, root system is soaked given birth to before planting Root liquid 5S；
Wherein taking root liquid is that root-inducing powder is watered into concentration is 20%wt；Also it is added with 0.85%wt mono-nitration guaiacol Sodium, 85%wt ethanol, 0.32%wt methyl α-naphthyl acetates, and the taking root liquid of addition, mono-nitration guaiacol sodium, ethanol, methyl α-naphthyl acetate Volume ratio is 10:2:2.5:2；
(2) soil cultivation：Select the physical features gradient be 20 ° of mountain region, the depth turned over is 70cm, then rake the soil level it is smooth to ground, And dig gutter；
(3) plant：Implantation time is in October, spacing in the rows 3.5*3m, digs cave, and the specification in cave is wide 15cm, high 15cm, field planting When will soak in planting seedlings and cave after taking root liquid, then earthed up, the graft union of seedling basseted 5cm, training Soil is a base diameter 80cm, is highly 20cm hill, and pours root water in time, is then spread on the surface of hill Last layer thickness 3cm fine sand；
(4) water and fertilizer management：When crown height is to 0.9m, every plant of application organic fertilizer 5kg is annual after the Second Year of field planting Autumn centered on tree crown, using 3m as diameter ditching, applies 15kg every plant of organic fertilizer；
The organic fertilizer is that following raw material is prepared：5 parts by weight coprinus comatus leftover bits and pieces, 0.75 parts by weight mulberry strangle careless, 0.75 parts by weight folium cortex eucommiae, 0.75 parts by weight selenocystein, 0.55 parts by weight of soybean shell, 1.75 parts by weight garlics, 1.35 weights Measure part oriental sesame pericarp, 0.95 parts by weight fructus cannabis, 0.85 parts by weight silkworm chrysalis, the compound beneficial bacterium of 0.5 parts by weight, 0.25 parts by weight honeybee Honey, 0.55 parts by weight of lemon, 0.65 parts by weight indigo plant plum；
The raw material that the compound beneficial bacterium includes following parts by weight is prepared into：0.3 part of lactic acid bacterial liquid, Dipel It is 0.25 part of bacterium solution, 0.45 part of Lactobacillus plantarum bacterium solution, 0.20 part of yellow mould bacterium solution, 0.35 part of lactobacillus bulgaricus bacterium solution, withered Careless 0.30 part of bacillus bacterium solution；
Effective bacteria containing amount of lactic acid bacterial liquid in the compound beneficial bacterium is 1.9 × 109Individual/mL, Dipel bacterium Effective bacteria containing amount of liquid is 4.16 × 108Individual/mL, effective bacteria containing amount of Lactobacillus plantarum bacterium solution are 3.26 × 109Individual/mL, Huang are mould Effective bacteria containing amount of bacterium bacterium solution is 2.58 × 109Individual/mL, effective bacteria containing amount of lactobacillus bulgaricus bacterium solution are 3.69 × 109 Individual/mL, effective bacteria containing amount of bacillus subtilis bacterium solution are 3.42 × 109Individual/mL.
(5) trimming leaf and shield flower and fruit：After fruit picking and before annual rudiment in April, cut off and intersect branch, weak branch, disease pest Branch and deadwood；The long shoot of summer sprouting is cut off, removes diseased plant in time；
(6) fruit picking and manage after adopting：Harvested in time after fruit maturation, it is shallow to planting site progress every 1 year during plantation Turn over, and improved the soil with quick lime.
The preparation method of the organic fertilizer of the application is：
Sang Lecao is extracted 10 minutes by chopping with water, filtering, is extracted 3 times altogether, is then obtained content and is reached 65% Asia The extract solution of oleic acid content；
Folium cortex eucommiae crushes after being dried, by circulating ultrasonic extraction element according to comminuted powder and solvent for extraction according to body Product is than being 1:2 ratio mixing extraction, 55 degrees Celsius, supersonic frequency 60KHz of Extracting temperature, is extracted 5 minutes, circulation extraction 5 It is secondary, 5 liquid are merged after filtering, liquid loads solvent recovery 60 degrees Celsius of recycling designs of enrichment facility, is then in pressure 15MPa, temperature are 35 DEG C, CO2Flow 15kg/h, time are 15 minutes, refined filtration, bark of eucommia leaf extract, in extract into Divide containing linoleic acid 59.3%, leukotrienes 3.6%；
Fructus cannabis is volatilized, is freeze-dried by broken, soxhlet extraction degreasing, water extract-alcohol precipitation, centrifugation, water-bath, produces purple perilla Seed extract, main component are that linoleic acid (ω -6 systems)/alpha-linolenic acid (ω -3 systems) ratio is 69%/12%.
Further illustrate, the lemon adds water and cellulase, volume ratio 1 after carrying out peeling remove seed:1:0.05, carry out Enzymolysis, then after carrying out enzyme deactivation, obtain enzymolysis liquid, the pH of enzymolysis liquid is adjusted to after 6, is extracted with ethyl acetate, take organic Phase, the 3wt.% of weight such as addition sodium hydrate aqueous solution, stirs, is extracted in organic phase, phase of fetching water, by water Mutually it is sent into milipore filter to be filtered, then the penetrating fluid of milipore filter is concentrated with NF membrane, then after being dried, obtains lemon Lemon chromocor extract；
Lan Mei, which is added in ethanol solution, carries out refluxing extraction 5 times, and then reclaiming concentrate is centrifuged, and takes supernatant Liquid, then add diatomite mixing and carry out press filtration, filtrate is filtered by being mounted with the resin of poly (ether sulfone) film material, filter is cut Molecular weight 5KD is stayed, then progressive is washed, and obtains eluent, and it is 1.12 times i.e. available that eluent, which is carried out being concentrated into volume, Blueberry chromocor extract；
Then coprinus comatus leftover bits and pieces, garlic, soybean peel, oriental sesame pericarp, silkworm chrysalis, honey are obtained after building heap and fermenting 10 days Basic fertilizer, continue to add Sang Lecao extract solutions, eucommia leaf extract, lemon chromocor extract, blue plum chromocor extract, the Guang of selenium half Propylhomoserin is mixed with basic fertilizer, continues to build heap fermentation 5 days.
Embodiment 3：
A kind of implantation methods of fertile mandarin orange, specifically include following steps,
(1) seedling process：Select disease-free, root system completely to irrigate the strain of mandarin orange seedling as seedling, root system is soaked given birth to before planting Root liquid 4S；
Wherein taking root liquid is that root-inducing powder is watered into concentration is 14%wt；Also it is added with 0.65%wt mono-nitration guaiacol Sodium, 80%wt ethanol, 0.29%wt methyl α-naphthyl acetates, and the taking root liquid of addition, mono-nitration guaiacol sodium, ethanol, methyl α-naphthyl acetate Volume ratio is 10:1.5:2:1.5；
(2) soil cultivation：Select the physical features gradient be 20 ° of mountain region, the depth turned over is 68cm, then rake the soil level it is smooth to ground, And dig gutter；
(3) plant：Implantation time is in September, spacing in the rows 3.2*2.6m, digs cave, and the specification in cave is wide 15cm, high 15cm, fixed It will be soaked when plant in planting seedlings and cave after taking root liquid, then earthed up, the graft union of seedling is basseted 4cm, Ridging is a base diameter 76cm, is highly 19cm hill, and pours root water in time, then on the surface of hill Sprinkle the fine sand of a thickness 2.5cm；
(4) water and fertilizer management：When crown height is to 0.9m, every plant of application organic fertilizer 4kg is annual after the Second Year of field planting Autumn centered on tree crown, using 2m as diameter ditching, applies 12kg every plant of organic fertilizer；
The organic fertilizer is that following raw material is prepared：1.5 parts by weight coprinus comatus leftover bits and pieces, 0.4 parts by weight mulberry strangle careless, 0.5 parts by weight folium cortex eucommiae, 0.4 parts by weight selenocystein, 0.2 parts by weight of soybean shell, 0.95 parts by weight garlic, 0.7 parts by weight Oriental sesame pericarp, 0.5 parts by weight fructus cannabis, 0.3 parts by weight silkworm chrysalis, the compound beneficial bacterium of 0.2 parts by weight, 0.15 parts by weight honey, 0.3 weight Measure part lemon, 0.4 parts by weight indigo plant plum；
The raw material that the compound beneficial bacterium includes following parts by weight is prepared into：0.25 part of lactic acid bacterial liquid, Su Yun gold bars It is 0.2 part of bacterium bacterium solution, 0.36 part of Lactobacillus plantarum bacterium solution, 0.15 part of yellow mould bacterium solution, 0.28 part of lactobacillus bulgaricus bacterium solution, withered Careless 0.25 part of bacillus bacterium solution.
Effective bacteria containing amount of lactic acid bacterial liquid in the compound beneficial bacterium is 5.5 × 108Individual/mL, Dipel bacterium Effective bacteria containing amount of liquid is 3.85 × 108Individual/mL, effective bacteria containing amount of Lactobacillus plantarum bacterium solution are 1.55 × 109Individual/mL, Huang are mould Effective bacteria containing amount of bacterium bacterium solution is 2.15 × 109Individual/mL, effective bacteria containing amount of lactobacillus bulgaricus bacterium solution are 3.10 × 109 Individual/mL, effective bacteria containing amount of bacillus subtilis bacterium solution are 1.25 × 109Individual/mL.
(5) trimming leaf and shield flower and fruit：After fruit picking and before annual rudiment in 2 months, cut off and intersect branch, weak branch, disease pest Branch and deadwood；The long shoot of summer sprouting is cut off, removes diseased plant in time；
(6) fruit picking and manage after adopting：Harvested in time after fruit maturation, it is shallow to planting site progress every 1 year during plantation Turn over, and improved the soil with quick lime.
The preparation method of the organic fertilizer of the application is：
Sang Lecao is extracted 10 minutes by chopping with water, filtering, is extracted 3 times altogether, is then obtained content and is reached 55% Asia The extract solution of oleic acid content；
Folium cortex eucommiae crushes after being dried, by circulating ultrasonic extraction element according to comminuted powder and solvent for extraction according to body Product is than being 1:2 ratio mixing extraction, 53 degrees Celsius, supersonic frequency 55KHz of Extracting temperature, is extracted 4 minutes, circulation extraction 5 It is secondary, 5 liquid are merged after filtering, liquid loads solvent recovery 58 degrees Celsius of recycling designs of enrichment facility, is then in pressure 12MPa, temperature are 32 DEG C, CO2Flow 15kg/h, time are 15 minutes, refined filtration, bark of eucommia leaf extract, in extract into Divide containing linoleic acid 59.3%, leukotrienes 3.6%；
Fructus cannabis is volatilized, is freeze-dried by broken, soxhlet extraction degreasing, water extract-alcohol precipitation, centrifugation, water-bath, produces purple perilla Seed extract, main component are that linoleic acid (ω -6 systems)/alpha-linolenic acid (ω -3 systems) ratio is 69%/12%.
Further illustrate, the lemon adds water and cellulase, volume ratio 1 after carrying out peeling remove seed:1:0.03, carry out Enzymolysis, then after carrying out enzyme deactivation, obtain enzymolysis liquid, the pH of enzymolysis liquid is adjusted to after 5, is extracted with ethyl acetate, take organic Phase, the 2wt.% of weight such as addition sodium hydrate aqueous solution, stirs, is extracted in organic phase, phase of fetching water, by water Mutually it is sent into milipore filter to be filtered, then the penetrating fluid of milipore filter is concentrated with NF membrane, then after being dried, obtains lemon Lemon chromocor extract；
Lan Mei, which is added in ethanol solution, carries out refluxing extraction 3 times, and then reclaiming concentrate is centrifuged, and takes supernatant Liquid, then add diatomite mixing and carry out press filtration, filtrate is filtered by being mounted with the resin of poly (ether sulfone) film material, filter is cut Molecular weight 5KD is stayed, then progressive is washed, and obtains eluent, and it is 1.12 times i.e. available that eluent, which is carried out being concentrated into volume, Blueberry chromocor extract；
Then coprinus comatus leftover bits and pieces, garlic, soybean peel, oriental sesame pericarp, silkworm chrysalis, honey are obtained after building heap and fermenting 9 days Basic fertilizer, continue to add Sang Lecao extract solutions, eucommia leaf extract, lemon chromocor extract, blue plum chromocor extract, the Guang of selenium half Propylhomoserin is mixed with basic fertilizer, continues to build heap fermentation 4 days.
Embodiment 4：
A kind of implantation methods of fertile mandarin orange, specifically include following steps,
(1) seedling process：Select disease-free, root system completely to irrigate the strain of mandarin orange seedling as seedling, root system is soaked given birth to before planting Root liquid 3S；
Wherein taking root liquid is that root-inducing powder is watered into concentration is 18%wt；Also it is added with 0.80%wt mono-nitration guaiacol Sodium, 84%wt ethanol, 0.30%wt methyl α-naphthyl acetates, and the taking root liquid of addition, mono-nitration guaiacol sodium, ethanol, methyl α-naphthyl acetate Volume ratio is 10:1.8:2.2:1.8；
(2) soil cultivation：Select the physical features gradient be 20 ° of mountain region, the depth turned over is 69cm, then rake the soil level it is smooth to ground, And dig gutter；
(3) plant：Implantation time is in September, spacing in the rows 3.4*2.9m, digs cave, and the specification in cave is wide 15cm, high 15cm, fixed By in the planting seedlings and cave after immersion taking root liquid when plant, then earthed up, the graft union of seedling is basseted 4.8cm, ridging is a base diameter 79cm, is highly 19cm hill, and pours root water in time, then in hill Surface sprinkle the fine sand of a thickness 2.8cm；
(4) water and fertilizer management：When crown height is to 0.9m, every plant of application organic fertilizer 4.5kg is annual after the Second Year of field planting Autumn centered on tree crown, using 2m as diameter ditching, apply 14.5kg every plant of organic fertilizer；
The organic fertilizer is that following raw material is prepared：4 parts by weight coprinus comatus leftover bits and pieces, 0.6 parts by weight mulberry strangle careless, 0.6 parts by weight folium cortex eucommiae, 0.6 parts by weight selenocystein, 0.6 parts by weight of soybean shell, 1.5 parts by weight garlics, 1.2 parts by weight sesames Numb shell, 0.8 parts by weight fructus cannabis, 0.7 parts by weight silkworm chrysalis, the compound beneficial bacterium of 0.4 parts by weight, 0.2 parts by weight honey, 0.5 weight Part lemon, 0.6 parts by weight indigo plant plum；
The raw material that the compound beneficial bacterium includes following parts by weight is prepared into：0.15 part of lactic acid bacterial liquid, Su Yun gold bars 0.18 part of bacterium bacterium solution, 0.39 part of Lactobacillus plantarum bacterium solution, 0.12 part of yellow mould bacterium solution, 0.27 part of lactobacillus bulgaricus bacterium solution, 0.20 part of bacillus subtilis bacterium solution.
Effective bacteria containing amount of lactic acid bacterial liquid in the compound beneficial bacterium is 0.55 × 109Individual/mL, Dipel bacterium Effective bacteria containing amount of liquid is 3.88 × 108Individual/mL, effective bacteria containing amount of Lactobacillus plantarum bacterium solution are 1.55 × 109Individual/mL, Huang are mould Effective bacteria containing amount of bacterium bacterium solution is 1.85 × 109Individual/mL, effective bacteria containing amount of lactobacillus bulgaricus bacterium solution are 2.06 × 109 Individual/mL, effective bacteria containing amount of bacillus subtilis bacterium solution are 0.68 × 109Individual/mL.
(5) trimming leaf and shield flower and fruit：After fruit picking and before annual rudiment in March, cut off and intersect branch, weak branch, disease pest Branch and deadwood；The long shoot of summer sprouting is cut off, removes diseased plant in time；
(6) fruit picking and manage after adopting：Harvested in time after fruit maturation, it is shallow to planting site progress every 1 year during plantation Turn over, and improved the soil with quick lime.
The preparation method of the organic fertilizer of the application is：
Sang Lecao is extracted 10 minutes by chopping with water, filtering, is extracted 3 times altogether, is then obtained content and is reached 59% Asia The extract solution of oleic acid content；
Folium cortex eucommiae crushes after being dried, by circulating ultrasonic extraction element according to comminuted powder and solvent for extraction according to body Product is than being 1:2 ratio mixing extraction, 51 degrees Celsius, supersonic frequency 59KHz of Extracting temperature, is extracted 5 minutes, circulation extraction 5 It is secondary, 5 liquid are merged after filtering, liquid loads solvent recovery 59 degrees Celsius of recycling designs of enrichment facility, is then in pressure 11MPa, temperature are 34 DEG C, CO2Flow 15kg/h, time are 15 minutes, refined filtration, bark of eucommia leaf extract, in extract into Divide containing linoleic acid 59.3%, leukotrienes 3.6%；
Fructus cannabis is volatilized, is freeze-dried by broken, soxhlet extraction degreasing, water extract-alcohol precipitation, centrifugation, water-bath, produces purple perilla Seed extract, main component are that linoleic acid (ω -6 systems)/alpha-linolenic acid (ω -3 systems) ratio is 69%/12%.
Further illustrate, the lemon adds water and cellulase, volume ratio 1 after carrying out peeling remove seed:1:0.04, carry out Enzymolysis, then after carrying out enzyme deactivation, obtain enzymolysis liquid, the pH of enzymolysis liquid is adjusted to after 5, is extracted with ethyl acetate, take organic Phase, the 2wt.% of weight such as addition sodium hydrate aqueous solution, stirs, is extracted in organic phase, phase of fetching water, by water Mutually it is sent into milipore filter to be filtered, then the penetrating fluid of milipore filter is concentrated with NF membrane, then after being dried, obtains lemon Lemon chromocor extract；
Lan Mei, which is added in ethanol solution, carries out refluxing extraction 4 times, and then reclaiming concentrate is centrifuged, and takes supernatant Liquid, then add diatomite mixing and carry out press filtration, filtrate is filtered by being mounted with the resin of poly (ether sulfone) film material, filter is cut Molecular weight 5KD is stayed, then progressive is washed, and obtains eluent, and it is 1.12 times i.e. available that eluent, which is carried out being concentrated into volume, Blueberry chromocor extract；
Then coprinus comatus leftover bits and pieces, garlic, soybean peel, oriental sesame pericarp, silkworm chrysalis, honey are obtained after building heap and fermenting 8 days Basic fertilizer, continue to add Sang Lecao extract solutions, eucommia leaf extract, lemon chromocor extract, blue plum chromocor extract, the Guang of selenium half Propylhomoserin is mixed with basic fertilizer, continues to build heap fermentation 4 days.
Embodiment 5：
A kind of implantation methods of fertile mandarin orange, specifically include following steps,
(1) seedling process：Select disease-free, root system completely to irrigate the strain of mandarin orange seedling as seedling, root system is soaked given birth to before planting Root liquid 3S；
Wherein taking root liquid is that root-inducing powder is watered into concentration is 14%wt；Also it is added with 0.65%wt mono-nitration guaiacol Sodium, 80%wt ethanol, 0.30%wt methyl α-naphthyl acetates, and the taking root liquid of addition, mono-nitration guaiacol sodium, ethanol, methyl α-naphthyl acetate Volume ratio is 10:1.7:2.2:1.6；
(2) soil cultivation：Select the physical features gradient be 20 ° of mountain region, the depth turned over is 68cm, then rake the soil level it is smooth to ground, And dig gutter；
(3) plant：Implantation time is in September, spacing in the rows 3.3*2.8m, digs cave, and the specification in cave is wide 15cm, high 15cm, fixed It will be soaked when plant in planting seedlings and cave after taking root liquid, then earthed up, the graft union of seedling is basseted 4cm, Ridging is a base diameter 78cm, is highly 19cm hill, and pours root water in time, then on the surface of hill Sprinkle the fine sand of a thickness 2.5cm；
(4) water and fertilizer management：When crown height is to 0.9m, every plant of application organic fertilizer 4kg is annual after the Second Year of field planting Autumn centered on tree crown, using 2m as diameter ditching, applies 13kg every plant of organic fertilizer；
The organic fertilizer is that following raw material is prepared：2.8 parts by weight coprinus comatus leftover bits and pieces, 0.5 parts by weight mulberry strangle careless, 0.55 parts by weight folium cortex eucommiae, 0.5 parts by weight selenocystein, 0.4 parts by weight of soybean shell, 1.2 parts by weight garlics, 0.9 parts by weight Oriental sesame pericarp, 0.6 parts by weight fructus cannabis, 0.5 parts by weight silkworm chrysalis, the compound beneficial bacterium of 0.3 parts by weight, 0.18 parts by weight honey, 0.4 weight Measure part lemon, 0.5 parts by weight indigo plant plum；
The raw material that the compound beneficial bacterium includes following parts by weight is prepared into：0.28 part of lactic acid bacterial liquid, Su Yun gold bars 0.22 part of bacterium bacterium solution, 0.42 part of Lactobacillus plantarum bacterium solution, 0.18 part of yellow mould bacterium solution, 0.32 part of lactobacillus bulgaricus bacterium solution, 0.20 part of bacillus subtilis bacterium solution.
Effective bacteria containing amount of lactic acid bacterial liquid in the compound beneficial bacterium is 5.5 × 108Individual/mL, Dipel bacterium Effective bacteria containing amount of liquid is 3.86 × 108Individual/mL, effective bacteria containing amount of Lactobacillus plantarum bacterium solution are 8.50 × 108Individual/mL, Huang are mould Effective bacteria containing amount of bacterium bacterium solution is 1.75 × 109Individual/mL, effective bacteria containing amount of lactobacillus bulgaricus bacterium solution are 7.55 × 109 Individual/mL, effective bacteria containing amount of bacillus subtilis bacterium solution are 6.85 × 109Individual/mL.
(5) trimming leaf and shield flower and fruit：After fruit picking and before annual rudiment in April, cut off and intersect branch, weak branch, disease pest Branch and deadwood；The long shoot of summer sprouting is cut off, removes diseased plant in time；
(6) fruit picking and manage after adopting：Harvested in time after fruit maturation, it is shallow to planting site progress every 1 year during plantation Turn over, and improved the soil with quick lime.
The preparation method of the organic fertilizer of the application is：
Sang Lecao is extracted 10 minutes by chopping with water, filtering, is extracted 3 times altogether, is then obtained content and is reached 62% Asia The extract solution of oleic acid content；
Folium cortex eucommiae crushes after being dried, by circulating ultrasonic extraction element according to comminuted powder and solvent for extraction according to body Product is than being 1:2 ratio mixing extraction, 54 degrees Celsius, supersonic frequency 59KHz of Extracting temperature, is extracted 5 minutes, circulation extraction 5 It is secondary, 5 liquid are merged after filtering, liquid loads solvent recovery 53 degrees Celsius of recycling designs of enrichment facility, is then in pressure 13MPa, temperature are 34 DEG C, CO2Flow 15kg/h, time are 15 minutes, refined filtration, bark of eucommia leaf extract, in extract into Divide containing linoleic acid 59.3%, leukotrienes 3.6%；
Fructus cannabis is volatilized, is freeze-dried by broken, soxhlet extraction degreasing, water extract-alcohol precipitation, centrifugation, water-bath, produces purple perilla Seed extract, main component are that linoleic acid (ω -6 systems)/alpha-linolenic acid (ω -3 systems) ratio is 69%/12%.
Further illustrate, the lemon adds water and cellulase, volume ratio 1 after carrying out peeling remove seed:1:0.04, carry out Enzymolysis, then after carrying out enzyme deactivation, obtain enzymolysis liquid, the pH of enzymolysis liquid is adjusted to after 4, is extracted with ethyl acetate, take organic Phase, the 1wt.% of weight such as addition sodium hydrate aqueous solution, stirs, is extracted in organic phase, phase of fetching water, by water Mutually it is sent into milipore filter to be filtered, then the penetrating fluid of milipore filter is concentrated with NF membrane, then after being dried, obtains lemon Lemon chromocor extract；
Lan Mei, which is added in ethanol solution, carries out refluxing extraction 2 times, and then reclaiming concentrate is centrifuged, and takes supernatant Liquid, then add diatomite mixing and carry out press filtration, filtrate is filtered by being mounted with the resin of poly (ether sulfone) film material, filter is cut Molecular weight 5KD is stayed, then progressive is washed, and obtains eluent, and it is 1.12 times i.e. available that eluent, which is carried out being concentrated into volume, Blueberry chromocor extract；
Then coprinus comatus leftover bits and pieces, garlic, soybean peel, oriental sesame pericarp, silkworm chrysalis, honey are obtained after building heap and fermenting 10 days Basic fertilizer, continue to add Sang Lecao extract solutions, eucommia leaf extract, lemon chromocor extract, blue plum chromocor extract, the Guang of selenium half Propylhomoserin is mixed with basic fertilizer, continues to build heap fermentation 3 days.
Carry out the measure of Se content to 1-5 groups embodiment and control group, assay method with reference to national standard GB5009.93- 2010, specific assay method is as follows：
(1) preparation of standard curve：
0.00mL is taken respectively, and 0.10mL, 0.20mL, 0.30mL, 0.40mL, 0.50mL standard liquids are in 15mL centrifuge tubes 10mL is settled to deionized water, then adds the hydrochloric acid 2mL, 100g/L of top pure grade potassium ferricyanide solution 1.0mL respectively, mixes system Into standard working curve.
(2) sample：
Take each 5g of citrus pulp of 1-5 groups embodiment and control group to be brewed with 100mLD boiling water, stand 3min, respectively Take supernatant to draw 10.00mL in ground conical flask, adding 10mL 5% to remove selenium sulfuric acid, after sample moistening, then add 20mL to mix Close acid solution to stand overnight, next day puts and is gradually heated up on electric hot plate.After vigorous reaction occurs, solution is in colourless, continues to be heated to White cigarette produces, and now solution gradually becomes faint yellow, i.e., up to terminal.
(3) determine：
Above-mentioned postdigestive sample solution adds 20.0mL EDTA mixed liquors, with the ammoniacal liquor of (1+1) and the hydrochloric acid of (1+9) It is adjusted to rutilous (pH 1.5~2.0).Following steps are in dark room operation：Add 1.0g/L DAN reagent 3.0mL, after mixing, put 5min is heated in boiling water bath, after taking out cooling, adds hexamethylene 3.0mL, shakes 4min, complete soln is moved into separatory funnel, treated Water layer is discarded after layering, carefully hexamethylene layer is poured into test tube with cover by separatory funnel is suitable for reading, does not make to be mixed into water in hexamethylene Drop, on sepectrophotofluorometer use excitation wavelength 376nm, wavelength of transmitted light 520nm measure 4,5-benzopiaselenol it is glimmering Luminous intensity.
(4) calculate：
Se content is calculated as follows formula and calculated in sample,
In formula：
X --- Se content in sample, unit are every gram of microgram or micrograms per millilitre (μ g/g or μ g/mL)；
m1--- the quality of selenium in test tube, unit are microgram (μ g)；
F1--- standard selenium fluorescence reading；
F2--- sample fluorescence reading；
F0--- blank tube fluorescence reading；
M --- sample mass, unit for gram or milliliter (g or mL).
Represented with the arithmetic mean of instantaneous value of the measurement result independent twice obtained under the conditions of repeatability, as a result retain three effectively Numeral.
Se content result see the table below：
Project Organic selenium content
Embodiment 1 15.112μg/kg
Embodiment 2 21.221μg/kg
Embodiment 3 18.344μg/kg
Embodiment 4 17.225μg/kg
Embodiment 5 20.305μg/kg
By embodiment 1-5 implantation methods, the place of plantation separates 6 mu of ground on the mountain region of Babu District and tested, often Mu is planted according to embodiment 1-5 respectively, wherein the 6th is planted using conventional plantation fertilizing method soon.When reaching Start to harvest after bearing fruit within 2 years, record every mu of the heavy amount of harvesting, the yield of embodiment 1-5 and control group is respectively 6871 very heavy Every mu, it is 7512 very heavy every mu, 6955 very heavy every mu, 6984 very heavy every mu, 7100 very heavy every mu, 5410 very heavy every mu, thus may be used See that the fertile mandarin orange yield of the method plantation using the present invention is high, the head of fruit generally is big, the high 56.8-68.9% of sugar content, Low 5-7% of acid content or so.Using the technical scheme of the application, generation pest and disease damage probability is small, and survival rate is high, and one increased income Individual key factor, and the probability that pest and disease damage occurs is few, the fruit bearing rate of fruit is compared to also more, and an important original increased income Cause.
Using the technical scheme of the application, small (every to impose organic fertilizer in embodiment every year, the generation of pest and disease damage probability occurs The probability statistics of disease see the table below), survival rate is high, and the key factor increased income, and the probability that pest and disease damage occurs is few, The fruit bearing rate of fruit is compared to also more, and a major reason increased income.
Remarks：Disease rate %=diseases area/666.7 square metre
The present processes and control group use the taking root liquid that common root-inducing powder is watered, and planting survival rates are suitable, use The taking root liquid of the application, compared to the advantages of be can be earlier allow fertile mandarin orange to recover, grow sprouting.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that for the person of ordinary skill of the art, Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.

Claims (7)

  1. A kind of 1. implantation methods of fertile mandarin orange, it is characterised in that：Specifically include following steps,
    (1) seedling process：Select disease-free, root system completely to irrigate the strain of mandarin orange seedling as seedling, root system is soaked into taking root liquid before planting 3-5S；
    (2) soil cultivation：Select the physical features gradient be 20 ° of mountain region, the depth turned over is 65-70cm, then rake the soil level it is smooth to ground, and Dig gutter；
    (3) plant：Implantation time is in the 9-10 months, spacing in the rows 3-3.5*2.5-3m, digs cave, and the specification in cave is wide 15cm, high 15cm, It will be soaked when field planting in planting seedlings and cave after taking root liquid, then earthed up, the graft union of seedling is basseted 3- 5cm, ridging is a base diameter 70-80cm, is highly 18-20cm hill, and pours root water in time, then small The surface of mound sprinkles the fine sand of a thickness 2-3cm；
    (4) water and fertilizer management：When crown height is to 0.9m, every plant of application organic fertilizer 3-5kg, the annual autumn after the Second Year of field planting Season centered on tree crown, using 2-3m as diameter ditching, applies 10-15kg every plant of organic fertilizer；
    The organic fertilizer is that following raw material is prepared：1-5 parts by weight coprinus comatus leftover bits and pieces, 0.3-0.75 parts by weight Sang Le Grass, 0.35-0.75 parts by weight folium cortex eucommiae, 0.3-0.75 parts by weight selenocystein, 0.15-0.55 parts by weight of soybean shell, 0.85- 1.75 parts by weight garlics, 0.5-1.35 parts by weight oriental sesame pericarp, 0.35-0.95 parts by weight fructus cannabis, 0.25-0.85 parts by weight silkworms Pupa, the compound beneficial bacterium of 0.1-0.5 parts by weight, 0.1-0.25 parts by weight honey, 0.25-0.55 parts by weight of lemon, 0.35-0.65 weights Measure part indigo plant plum；
    (5) trimming leaf and shield flower and fruit：After fruit picking and before annual 2-4 months rudiment, cut off and intersect branch, weak branch, sick branch And deadwood；The long shoot of summer sprouting is cut off, removes diseased plant in time；
    (6) fruit picking and manage after adopting：Harvested in time after fruit maturation, it is shallow to planting site progress every 1 year during plantation to turn over, and Improved the soil with quick lime.
  2. A kind of 2. implantation methods of fertile mandarin orange according to claim 1, it is characterised in that：The organic fertilizer is following raw material system For what is formed：1.5-4 parts by weight coprinus comatus leftover bits and pieces, 0.4-0.6 parts by weight mulberries strangle careless, 0.5-0.6 parts by weight folium cortex eucommiae, 0.4- 0.6 parts by weight selenocystein, 0.2-0.6 parts by weight of soybean shell, 0.95-1.5 parts by weight garlic, 0.7-1.2 parts by weight sesames Shell, 0.5-0.8 parts by weight fructus cannabis, 0.3-0.7 parts by weight silkworm chrysalis, the compound beneficial bacterium of 0.2-0.4 parts by weight, 0.15-0.2 weight Part honey, 0.3-0.5 parts by weight of lemon, 0.4-0.6 parts by weight indigo plant plums.
  3. A kind of 3. implantation methods of fertile mandarin orange according to claim 1, it is characterised in that：The organic fertilizer is following raw material system For what is formed：2.8 parts by weight coprinus comatus leftover bits and pieces, 0.5 parts by weight mulberry strangle careless, 0.55 parts by weight folium cortex eucommiae, 0.5 parts by weight selenium half Cystine, 0.4 parts by weight of soybean shell, 1.2 parts by weight garlics, 0.9 parts by weight oriental sesame pericarp, 0.6 parts by weight fructus cannabis, 0.5 weight Part silkworm chrysalis, the compound beneficial bacterium of 0.3 parts by weight, 0.18 parts by weight honey, 0.4 parts by weight of lemon, 0.5 parts by weight indigo plant plum.
  4. A kind of 4. implantation methods of fertile mandarin orange according to claim 1, it is characterised in that：The compound beneficial bacterium includes following The raw material of parts by weight is prepared into：Lactic acid bacterial liquid 0.1-0.3 parts, Dipel bacterium solution 0.15-0.25 parts, Lactobacillus plantarum Bacterium solution 0.31-0.45 parts, yellow mould bacterium solution 0.10-0.20 parts, lactobacillus bulgaricus bacterium solution 0.20-0.35 parts, withered grass gemma Bacillus bacterium solution 0.15-0.30 parts.
  5. A kind of 5. implantation methods of fertile mandarin orange according to claim 1, it is characterised in that：The compound beneficial bacterium includes following The raw material of parts by weight is prepared into：0.25 part of lactic acid bacterial liquid, 0.2 part of Dipel bacterium solution, Lactobacillus plantarum bacterium solution 0.36 Part, 0.15 part of yellow mould bacterium solution, 0.28 part of lactobacillus bulgaricus bacterium solution, 0.25 part of bacillus subtilis bacterium solution.
  6. A kind of 6. implantation methods of fertile mandarin orange according to claim 4 or 5, it is characterised in that：In the compound beneficial bacterium Effective bacteria containing amount of lactic acid bacterial liquid is 3.8 × 108-1.9×109Individual/mL, effective bacteria containing amount of Dipel bacterium solution are 3.25×108-4.16×108Individual/mL, effective bacteria containing amount of Lactobacillus plantarum bacterium solution are 2.98 × 108-3.26×109Individual/mL, Effective bacteria containing amount of yellow mould bacterium solution is 1.65 × 109-2.58×109Individual/mL, the effective of lactobacillus bulgaricus bacterium solution contain bacterium Measure as 2.89 × 108-3.69×109Individual/mL, effective bacteria containing amount of bacillus subtilis bacterium solution are 2.56 × 108-3.42×109 Individual/mL.
  7. A kind of 7. implantation methods of fertile mandarin orange according to claim 1, it is characterised in that：The taking root liquid is to convert root-inducing powder Water into concentration be 10%wt-20%wt；Also added with 0.55%wt-0.85%wt mono-nitration guaiacol sodium, 75%wt-85% Wt ethanol, 0.25%wt-0.32%wt methyl α-naphthyl acetates, and taking root liquid, mono-nitration guaiacol sodium, ethanol, the methyl α-naphthyl acetate added Volume ratio be 10:1-2:1.5-2.5:1-2.
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CN108476839A (en) * 2018-02-08 2018-09-04 马焰琳 A kind of planting technology of selenium-rich mandarin orange fruit
CN108551964A (en) * 2018-07-19 2018-09-21 安吉仕锦农业发展有限公司 A kind of implantation methods of nutrition cumquat
CN108566864A (en) * 2018-07-19 2018-09-25 安吉仕锦农业发展有限公司 A kind of implantation methods of cumquat
CN108781987A (en) * 2018-07-19 2018-11-13 安吉仕锦农业发展有限公司 A kind of cumquat implantation methods
CN108901558A (en) * 2018-07-20 2018-11-30 博白县百家水果专业合作社 A kind of implantation methods of fertile mandarin orange
CN109121775A (en) * 2018-07-20 2019-01-04 博白县百家水果专业合作社 A method of cultivating fertile mandarin orange seedling
CN109121939A (en) * 2018-07-20 2019-01-04 博白县百家水果专业合作社 A kind of implantation methods for preventing and treating fertile mandarin orange aphid
CN111615987A (en) * 2020-06-30 2020-09-04 大理万众苑科技有限公司 Planting method of Or

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103360138A (en) * 2013-04-18 2013-10-23 湖北宏全生物科技有限公司 Production and preparation method of selenium-rich biological organic fertilizer
CN104774045A (en) * 2015-04-10 2015-07-15 民勤县拓普草业有限公司 Method for processing microbial organic bacterial fertilizer from Sorghum vulgare wort
CN105418172A (en) * 2016-01-11 2016-03-23 鹤壁市人元生物技术发展有限公司 Preparation method of biological organic fertilizer
CN106034947A (en) * 2016-06-27 2016-10-26 博白县锦兴水果种植专业合作社 Citrus reiculata Blanco high-yield planting method
CN106069479A (en) * 2016-06-17 2016-11-09 博白县锦兴水果种植专业合作社 A kind of implantation methods of fertile Citrus chachiensis Hort.
CN106069480A (en) * 2016-06-17 2016-11-09 博白县锦兴水果种植专业合作社 A kind of implantation methods of fertile Citrus chachiensis Hort.

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103360138A (en) * 2013-04-18 2013-10-23 湖北宏全生物科技有限公司 Production and preparation method of selenium-rich biological organic fertilizer
CN104774045A (en) * 2015-04-10 2015-07-15 民勤县拓普草业有限公司 Method for processing microbial organic bacterial fertilizer from Sorghum vulgare wort
CN105418172A (en) * 2016-01-11 2016-03-23 鹤壁市人元生物技术发展有限公司 Preparation method of biological organic fertilizer
CN106069479A (en) * 2016-06-17 2016-11-09 博白县锦兴水果种植专业合作社 A kind of implantation methods of fertile Citrus chachiensis Hort.
CN106069480A (en) * 2016-06-17 2016-11-09 博白县锦兴水果种植专业合作社 A kind of implantation methods of fertile Citrus chachiensis Hort.
CN106034947A (en) * 2016-06-27 2016-10-26 博白县锦兴水果种植专业合作社 Citrus reiculata Blanco high-yield planting method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
黄其椿: "《沃柑虽火，种植有讲究》", 《农家之友》 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108476839A (en) * 2018-02-08 2018-09-04 马焰琳 A kind of planting technology of selenium-rich mandarin orange fruit
CN108551964A (en) * 2018-07-19 2018-09-21 安吉仕锦农业发展有限公司 A kind of implantation methods of nutrition cumquat
CN108566864A (en) * 2018-07-19 2018-09-25 安吉仕锦农业发展有限公司 A kind of implantation methods of cumquat
CN108781987A (en) * 2018-07-19 2018-11-13 安吉仕锦农业发展有限公司 A kind of cumquat implantation methods
CN108901558A (en) * 2018-07-20 2018-11-30 博白县百家水果专业合作社 A kind of implantation methods of fertile mandarin orange
CN109121775A (en) * 2018-07-20 2019-01-04 博白县百家水果专业合作社 A method of cultivating fertile mandarin orange seedling
CN109121939A (en) * 2018-07-20 2019-01-04 博白县百家水果专业合作社 A kind of implantation methods for preventing and treating fertile mandarin orange aphid
CN111615987A (en) * 2020-06-30 2020-09-04 大理万众苑科技有限公司 Planting method of Or

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Application publication date: 20171124