CN105418172A - Preparation method of biological organic fertilizer - Google Patents
Preparation method of biological organic fertilizer Download PDFInfo
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- CN105418172A CN105418172A CN201610012339.2A CN201610012339A CN105418172A CN 105418172 A CN105418172 A CN 105418172A CN 201610012339 A CN201610012339 A CN 201610012339A CN 105418172 A CN105418172 A CN 105418172A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention provides a preparation method of a biological organic fertilizer. The preparation method comprises the steps of fermentation substrate providing, fermentation substrate stacking and substrate fermentation, wherein the step of fermentation substrate stacking comprises the steps that a fermentation substrate and a fermentation liquid microorganism bacterium agent are evenly mixed and stacked to a rectangular fermentation substrate pile with the height of 0.5-0.8 m according to the proportion that 0.9-1.1 L of the fermentation liquid microorganism bacterium agent is added to every 1,000 kg of the fermentation substrate. The fermentation liquid microorganism bacterium agent comprises bacillus subtilis liquid, bacillus licheniformis liquid, saccharomyces cerevisiae liquid, lactic acid bacillus liquid and aspergillus niger liquid. By means of the preparation method of the biological organic fertilizer, waste materials can be changed into treasure, and the generally existing environmental pollution problems that when resourceful treatment of organic solid waste is conducted, the capacity for decomposing macromolecular protein and coarse fibers is weak, nitrogen is prone to loss, and pungent odors are produced can be solved.
Description
Technical field
The present invention relates to fertilizer field, especially relate to a kind of fermented liq microbiobacterial agent and preparation method thereof and fermentation process.
Background technology
At present, China, in crop-planting, uses chemical fertilizer mostly, although have certain renting in increasing production, increasing income, but life-time service, following problem can be produced:
1, spoiled soil, the phenomenons such as organic content on the contrary in soil reduces, and causes nutritive ingredient out of proportion, and acidifying appears in soil, harden, affect the output of crop;
2, agroecological environment worsens, and chemical fertilizer is executed as behind farmland, and practical efficiency on average only has 30%, and major part flows in rivers with farmland drainage, contaminate environment, harm health of people.
Therefore, Current Domestic is outer all to be tried to explore for organic solid castoff recycling treatment aspect, has also occurred that a series of related microorganisms microbial inoculum ferments for organic solid castoff simultaneously.But in fermentation recycling treatment process, ubiquity decomposes high molecular weight protein and the weak and easy loss of nitrogen of robust fibre ability, produces the problems such as pungent peculiar smell.
Summary of the invention
Therefore, the object of this invention is to provide a kind of preparation method of biological organic fertilizer, not only can realize turning waste into wealth, can also solve in prior art when utilizing organic solid castoff recycling treatment, ubiquitous decomposition high molecular weight protein and robust fibre ability weak, and the easy loss of nitrogen, produce the problem of the contaminate environment such as pungent peculiar smell.
The technical scheme that the present invention takes is as follows:
A preparation method for biological organic fertilizer, it comprises the following steps:
There is provided fermentation substrate: the organic waste providing water content between 60%-70%, as fermentation substrate;
Stack fermentation substrate: the ratio adding the fermented liq microbiobacterial agent of 0.9-1.1L according to every 1000kg fermentation substrate, described fermentation substrate and described fermented liq microbiobacterial agent are carried out Homogeneous phase mixing, stack into the rectangle fermentation substrate heap of high 0.5-0.8m, wherein, described fermented liq microbiobacterial agent comprises following component: subtilis bacterium liquid, Bacillus licheniformis liquid, S. cervisiae liquid, lactobacillus bacterium liquid and black-koji mould liquid;
Fermenting substrate: described rectangle fermentation substrate piles up 48 DEG C-68 DEG C and ferments, until the loose and odorless of described fermentation substrate.
Wherein, " water content " herein refers to the mass percent that the moisture in organic waste occupies.
Based on above-mentioned, described organic waste is one or more in feces of livestock and poultry, stalk, edible fungi leftovers.
Based on above-mentioned, described fermentation substrate comprises the organic waste of following mass percent: feces of livestock and poultry 35%-45%, stalk 27.5%-32.5%, edible fungi leftovers 27.5%-32.5%, and described stalk and edible fungi leftovers are the particle of 3-5mm.
Based on above-mentioned, described fermented liq microbiobacterial agent comprises the component of following percent by volume: subtilis bacterium liquid 40%-50%, Bacillus licheniformis liquid 12%-18%, S. cervisiae liquid 16%-24%, lactobacillus bacterium liquid 7%-9% and black-koji mould liquid 10%-14%, wherein, the concentration of described subtilis bacterium liquid, described Bacillus licheniformis liquid, described S. cervisiae liquid, described lactobacillus bacterium liquid and described black-koji mould liquid is 9-11 hundred million cfu/ml.
Based on above-mentioned, the preparation method of described fermented liq microbiobacterial agent comprises the following steps:
Activation culture: Bacillus subtilis strain, Bacillus licheniformis strain, yeast saccharomyces cerevisiae bacterial classification, Lactobacillus species, aspergillus niger strain are inoculated into respectively in corresponding substratum and carry out activation culture, obtains generation bacterial strain respectively;
Enlarged culturing: the generation bacterial strain of described subtilis and the generation bacterial strain of described Bacillus licheniformis are inoculated in broth agar culture medium respectively and cultivate, obtain subtilis bacterium liquid and Bacillus licheniformis liquid respectively; The generation bacterial strain of described yeast saccharomyces cerevisiae, the generation bacterial strain of described aspergillus niger are inoculated into respectively in PDA substratum and cultivate, obtains S. cervisiae liquid and black-koji mould liquid respectively; Lactobacillus bacterium liquid is obtained by carrying out cultivation in the generation inoculation of described lactobacillus to MRS substratum.
Prepare microbial inoculum: described subtilis bacterium liquid, described Bacillus licheniformis liquid, described S. cervisiae liquid, described lactobacillus bacterium liquid and described black-koji mould liquid are mixed, obtains described fermented liq microbiobacterial agent.
Based on above-mentioned, the step of described activation culture comprises: adopt broth agar culture medium to carry out activation culture to described Bacillus subtilis strain and described Bacillus licheniformis strain respectively, obtain the generation bacterial strain of described subtilis and the generation bacterial strain of described Bacillus licheniformis respectively; Adopt PDA substratum to carry out activation culture to described yeast saccharomyces cerevisiae bacterial classification and described aspergillus niger strain respectively, obtain the generation bacterial strain of described yeast saccharomyces cerevisiae and the generation bacterial strain of described aspergillus niger respectively; Adopt MRS substratum to carry out activation culture to described Lactobacillus species, obtain described lactobacillus generation bacterial strain.
Based on above-mentioned, the culture temperature in described activation culture step is 29-31 DEG C, and the activation culture time is 20-28 hour.
Based on above-mentioned, described in the culture temperature that spreads cultivation in culturing step be 27-29 DEG C, the incubation time that spreads cultivation is 40-56 hour.
Wherein, the concentration of cultivating the described subtilis bacterium liquid obtained, described Bacillus licheniformis liquid, described S. cervisiae liquid, described lactobacillus bacterium liquid and described black-koji mould liquid in described activation culture step is 9-11 hundred million cfu/ml; Prepare in the step of microbial inoculum described, each bacterium liquid mixes according to following percent by volume: subtilis bacterium liquid 40%-50%, Bacillus licheniformis liquid 12%-18%, S. cervisiae liquid 16%-24%, lactobacillus bacterium liquid 7%-9% and black-koji mould liquid 10%-14%.
Based on above-mentioned, in the step of described fermenting substrate, the fermentation time of described rectangle fermentation substrate heap is 8-10 days.
The liquid microbe microbial inoculum that in the present invention, the mixing of five kinds of microorganisms obtains comparatively prior art can significantly improve feces of livestock and poultry, stalk, edible fungi leftovers fermentation efficiency, significantly improves feces of livestock and poultry, stalk, edible fungi leftovers resource utilization rate.In agricultural, livestock and poultry cultivation, there is good promotional value.Solve the environmental pollution of solid waste and the low problem of resource utilization rate simultaneously.
Compared with prior art, the preparation method of biological organic fertilizer provided by the invention mainly utilizes fermented liq microbiobacterial agent to carry out fermentation reaction and obtained biological organic fertilizer to organic solid castoff, and turn waste into wealth, Measures compare is simple, is easy to use, and, the fermented liq microbiobacterial agent that the present invention uses is that one mainly comprises subtilis, Bacillus licheniformis, yeast saccharomyces cerevisiae, the efficient cellulase-producing that five kinds of microorganisms such as lactobacillus and aspergillus niger are composite, the function and service microbial inoculum of proteolytic enzyme and odor control actives, between each microorganism in described fermented liq microbiobacterial agent, division of labor effect is clear and definite, for the high molecular weight protein being difficult to degrade and robust fibre, there is efficient degradation performance, stable strong, so, feces of livestock and poultry can be decomposed rapidly by high-efficiency environment friendly, stalk, the Mierocrystalline cellulose of the organic wastes such as edible fungi leftovers, high molecular weight protein, various complexing trace element, beneficial microorganism is rich in formation, organic, nitrogen, phosphorus, the efficient biologic-organic fertilizer of potassium and trace element, and make containing a large amount of useful viable bacterias, decomposition enzyme and biologically active factors in this biological organic fertilizer, effectively improve the organic matter in soil, reduce the generation of diseases and pests of agronomic crop.Therefore, the preparation method of biological organic fertilizer provided by the invention has a fermentation initial stage and heats up rapidly, and high-temperature duration is long, proteolytic enzyme and cellulase activity high and stable, fermentation time is short, and enzyme is lived by force, viable count is high, the features such as good deodorization effect; And in the preparation process of this biological organic fertilizer, not only the organic wastes such as feces of livestock and poultry, stalk, edible fungi leftovers are become biological organic fertilizer, turn waste into wealth, in whole preparation process, free from extraneous odour produces simultaneously, environmental friendliness, solves existing organic waste easily produces pungent peculiar smell etc. problem of environmental pollution in treating processes.
Further, the fermented liq microbiobacterial agent adopted in the preparation method of biological organic fertilizer provided by the invention is primarily of 45% subtilis bacterium liquid, 15% Bacillus licheniformis liquid, 20% S. cervisiae liquid, 8% lactobacillus bacterium liquid, the multi-functional composite fungus agent of 12% black-koji mould liquid composition, it is excellent that this fermented liq microbiobacterial agent has structure, effect is steady, enzyme is lived strong, viable count high, more can decompose feces of livestock and poultry rapidly by high-efficiency environment friendly, stalk, the various Mierocrystalline celluloses of the organic wastes such as edible fungi leftovers, high molecular weight protein, various complexing trace element, beneficial microorganism is rich in formation, organic, nitrogen, phosphorus, the efficient biologic-organic fertilizer of potassium and trace element, and pungent peculiar smell can not be produced.
Embodiment
Below by embodiment, technical scheme of the present invention is described in further detail.
Embodiment 1
The embodiment of the present invention 1 provides a kind of preparation method of biological organic fertilizer, and it comprises the following steps:
Fermentation substrate is provided: provide water content be 60% chicken manure just organic waste, as fermentation substrate;
Stack fermentation substrate: the ratio adding the fermented liq microbiobacterial agent of 0.9L according to every 1000kg fermentation substrate, after described fermentation substrate is mixed with described fermented liq microbiobacterial agent, again with 3 mixings of fertilizer turnover throwing machine, and stack into the rectangle fermentation substrate heap of high 0.5m;
Fermenting substrate: described rectangle fermentation substrate piles up 48-50 DEG C and ferments, the 8 days secondary fermentation substrates that ferment are loose, terminate fermentation, obtain biological organic fertilizer during odorless.
Wherein, the described fermented liq microbiobacterial agent adopted in the present embodiment comprises the component of following percent by volume: subtilis bacterium liquid 40%, Bacillus licheniformis liquid 18%, S. cervisiae liquid 23%, lactobacillus bacterium liquid 7% and black-koji mould liquid 10%, wherein, the concentration of described subtilis bacterium liquid, described Bacillus licheniformis liquid, described S. cervisiae liquid, described lactobacillus bacterium liquid and described black-koji mould liquid is 900,000,000 cfu/ml.The preparation method of this fermented liq microbiobacterial agent, comprises the following steps:
Activation culture: Bacillus subtilis strain, Bacillus licheniformis strain, yeast saccharomyces cerevisiae bacterial classification, Lactobacillus species, aspergillus niger strain to be inoculated into respectively in corresponding substratum 29 DEG C of activation culture 28 hours, to obtain generation bacterial strain respectively;
Enlarged culturing: the generation bacterial strain of described subtilis and the generation bacterial strain of described Bacillus licheniformis are inoculated in broth agar culture medium respectively, 27 DEG C of cultivations 56 hours that spread cultivation, obtain subtilis bacterium liquid that concentration is 900,000,000 cfu/ml and Bacillus licheniformis liquid respectively; The generation bacterial strain of described yeast saccharomyces cerevisiae, the generation bacterial strain of described aspergillus niger are inoculated in PDA substratum respectively, 27 DEG C of cultivations 56 hours that spread cultivation, obtain S. cervisiae liquid that concentration is 900,000,000 cfu/ml and black-koji mould liquid respectively; By the generation inoculation of described lactobacillus in MRS substratum, 27 DEG C of cultivations 56 hours that spread cultivation, obtain the lactobacillus bacterium liquid that concentration is 900,000,000 cfu/ml;
Prepare microbial inoculum: mix according to the formula of the above-mentioned fermented liq microbiobacterial agent adopted in the present embodiment, obtain above-mentioned fermented liq microbiobacterial agent.
The method adopting organic fertilizer NY525-2012 standard to specify is tested the described biological organic fertilizer that the preparation method provided by the present embodiment obtains, wherein, the mass percentage of the main ingredient in described biological organic fertilizer is as follows: organic 70%, purity nitrogen 2.11%, phosphorus (P
2o
5) 2.07%, potassium (K
2o) 0.85%, meet the standard of organic fertilizer, can directly be used as chicken manure organic fertilizer and use.
Embodiment 2
The embodiment of the present invention 2 provides a kind of preparation method of biological organic fertilizer, and it comprises the following steps:
Fermentation substrate is provided: provide water content be 63% organic waste, as fermentation substrate, wherein, described fermentation substrate is made up of the organic waste of following mass percent: the pig manure of 60% and the stalk of 40%, and described stalk and edible fungi leftovers are the particle of 3-5mm;
Stack fermentation substrate: the ratio adding the fermented liq microbiobacterial agent of 0.95L according to every 1000kg fermentation substrate, after described fermentation substrate is mixed with described fermented liq microbiobacterial agent, again with 4 mixings of fertilizer turnover throwing machine, and stack into the rectangle fermentation substrate heap of high 0.6-0.7m;
Fermenting substrate: described rectangle fermentation substrate piles up 52-54 DEG C and ferments, the 9 days secondary fermentation substrates that ferment are loose, terminate fermentation, obtain biological organic fertilizer during odorless.
Wherein, the described fermented liq microbiobacterial agent adopted in the present embodiment comprises the component of following percent by volume: subtilis bacterium liquid 42%, Bacillus licheniformis liquid 16%, S. cervisiae liquid 22%, lactobacillus bacterium liquid 7.5% and black-koji mould liquid 12.5%, wherein, the concentration of described subtilis bacterium liquid is 9.2 hundred million cfu/ml, the concentration of described Bacillus licheniformis liquid is 9.5 hundred million cfu/ml, the concentration of described S. cervisiae liquid is 1,000,000,000 cfu/ml, the concentration of described lactobacillus bacterium liquid is the concentration of 9.8 hundred million cfu/ml and described black-koji mould liquid is 9.3 hundred million cfu/ml.The preparation method of the above-mentioned fermented liq microbiobacterial agent that the present embodiment uses, the preparation method of the fermented liq microbiobacterial agent used with embodiment 1 is substantially identical, and difference is:
In the step of described activation culture, described Bacillus subtilis strain and Bacillus licheniformis strain are inoculated in broth agar culture medium respectively, 30 DEG C of activation culture 24 hours, obtain the generation bacterial strain of subtilis and the generation bacterial strain of Bacillus licheniformis respectively; Yeast saccharomyces cerevisiae bacterial classification and aspergillus niger strain are inoculated in PDA substratum respectively, 29 DEG C of activation culture 26 hours, obtain the generation bacterial strain of yeast saccharomyces cerevisiae and the generation bacterial strain of aspergillus niger respectively; Lactobacillus species is inoculated in MRS substratum, 29 DEG C of activation culture 24 hours, obtains lactobacillus generation bacterial strain;
In the step of described enlarged culturing, described subtilis, described Bacillus licheniformis, described yeast saccharomyces cerevisiae, the culture temperature that spreads cultivation of described aspergillus niger and described lactobacillus generation bacterial strain is separately 28 DEG C, the incubation time that spreads cultivation is 50 hours, the concentration of the described subtilis bacterium liquid finally obtained is 9.2 hundred million cfu/ml, the concentration of described Bacillus licheniformis liquid is 9.5 hundred million cfu/ml, the concentration of described S. cervisiae liquid is 1,000,000,000 cfu/ml, the concentration of described lactobacillus bacterium liquid is the concentration of 9.8 hundred million cfu/ml and described black-koji mould liquid is 9.3 hundred million cfu/ml,
Prepare in the step of microbial inoculum described, the formula of the fermented liq microbiobacterial agent used according to the present embodiment mixes.
The method adopting organic fertilizer NY525-2012 standard to specify is tested the described biological organic fertilizer that the preparation method provided by the present embodiment obtains, wherein, the mass percentage of the main ingredient in described biological organic fertilizer is as follows: organic 65%, purity nitrogen 1.97%, phosphorus (P
2o
5) 2.31%, potassium (K
2o) 1.85%, meet the standard of organic fertilizer, can directly be used as Swine manure and use.
Embodiment 3
The embodiment of the present invention 3 provides a kind of preparation method of biological organic fertilizer, and it comprises the following steps:
Fermentation substrate is provided: provide water content be 65% organic waste, as fermentation substrate, wherein, described fermentation substrate is made up of the organic waste of following mass percent: feces of livestock and poultry 40%, stalk 30%, edible fungi leftovers 30%, and described stalk and edible fungi leftovers are the particle of 3-5mm;
Stack fermentation substrate: the ratio adding the fermented liq microbiobacterial agent of 0.9L according to every 1000kg fermentation substrate, after described fermentation substrate is mixed with described fermented liq microbiobacterial agent, again with 3 mixings of fertilizer turnover throwing machine, and stack into the rectangle fermentation substrate heap of high 0.7-0.8m;
Fermenting substrate: described rectangle fermentation substrate piles up 57-59 DEG C and ferments, the 7 days secondary fermentation substrates that ferment are loose, terminate fermentation, obtain biological organic fertilizer during odorless.
Wherein, the described fermented liq microbiobacterial agent adopted in the present embodiment comprises the component of following percent by volume: subtilis bacterium liquid 45%, Bacillus licheniformis liquid 15%, S. cervisiae liquid 20%, lactobacillus bacterium liquid 8% and black-koji mould liquid 12%, wherein, the concentration of described subtilis bacterium liquid, described Bacillus licheniformis liquid, described S. cervisiae liquid, described lactobacillus bacterium liquid and described black-koji mould liquid is 1,000,000,000 cfu/ml.The preparation method of the above-mentioned fermented liq microbiobacterial agent that the present embodiment uses, the preparation method of the fermented liq microbiobacterial agent used with embodiment 2 is substantially identical, and difference is:
In the step of described activation culture, the activation culture temperature of five kinds of described microbial strainss is 30 DEG C, and the activation culture time is 24 hours;
In the step of described enlarged culturing, the generation bacterial strain of the described five kinds of micro-raw bacterium culture temperature that spreads cultivation respectively is 28 DEG C, and the incubation time that spreads cultivation is 48 hours, and the concentration of the bacterium liquid of the above-mentioned five kinds of micro-raw bacterium finally obtained is 1,000,000,000 cfu/ml;
Prepare in the step of microbial inoculum described, the formula of the fermented liq microbiobacterial agent used according to the present embodiment mixes.
The method adopting organic fertilizer NY525-2012 standard to specify is tested the biological organic fertilizer that the preparation method provided by the present embodiment obtains, wherein, the mass percentage of the main ingredient in described biological organic fertilizer is as follows: organic 63%, purity nitrogen 1.82%, phosphorus (P
2o
5) 1.93%, potassium (K
2o) 2.13%, pH value is 5.5-6.5, meets the standard of organic fertilizer, directly can be used as organic fertilizer application.
Embodiment 4
The embodiment of the present invention 4 provides a kind of preparation method of biological organic fertilizer, and it comprises the following steps:
Fermentation substrate is provided: provide water content be 70% chicken manure just organic waste, as fermentation substrate, wherein, described fermentation substrate is made up of the organic waste of following mass percent: feces of livestock and poultry 35%, stalk 32.5%, edible fungi leftovers 32.5%, and described stalk and edible fungi leftovers are the particle of 3-5mm;
Stack fermentation substrate: the ratio adding the fermented liq microbiobacterial agent of 1.1L according to every 1000kg fermentation substrate, after described fermentation substrate is mixed with described fermented liq microbiobacterial agent, again with 5 mixings of fertilizer turnover throwing machine, and stack into the rectangle fermentation substrate heap of high 0.6-0.7m;
Fermenting substrate: described rectangle fermentation substrate piles up 66-68 DEG C and ferments, the 8 days secondary fermentation substrates that ferment are loose, terminate fermentation, obtain biological organic fertilizer during odorless.
Wherein, the described fermented liq microbiobacterial agent adopted in the present embodiment comprises the component of following percent by volume: subtilis bacterium liquid 48%, Bacillus licheniformis liquid 14%, S. cervisiae liquid 18%, lactobacillus bacterium liquid 9% and black-koji mould liquid 11%, wherein, the concentration of described subtilis bacterium liquid is 10.2 hundred million cfu/ml, the concentration of described Bacillus licheniformis liquid is 1,000,000,000 cfu/ml, the concentration of described S. cervisiae liquid is 10.5 hundred million cfu/ml, the concentration of described lactobacillus bacterium liquid is the concentration of 9.9 hundred million cfu/ml and described black-koji mould liquid is 10.1 hundred million cfu/ml.The preparation method of the above-mentioned fermented liq microbiobacterial agent that the present embodiment uses, the preparation method of the fermented liq microbiobacterial agent used with embodiment 2 is substantially identical, and difference is:
In the step of described activation culture, the activation culture temperature of five kinds of described microbial strainss is 31 DEG C, and the activation culture time is 20 hours;
In the step of described enlarged culturing, the generation bacterial strain of the described five kinds of micro-raw bacterium culture temperature that spreads cultivation respectively is 29 DEG C, and the incubation time that spreads cultivation is 40 hours, and five kinds of micro-raw bacterium bacterium liquid of described concentration that the present embodiment finally obtained uses;
Prepare in the step of microbial inoculum described, the formula of the fermented liq microbiobacterial agent used according to the present embodiment mixes.
The method adopting organic fertilizer NY525-2012 standard to specify is tested the chicken manure described biological organic fertilizer obtained that ferments the fermented liq microbiobacterial agent utilizing the present embodiment to provide, wherein, the mass percentage of the main ingredient in described biological organic fertilizer is as follows: organic 61.2%, purity nitrogen 2.31%, phosphorus (P
2o
5) 2.81%, potassium (K
2o) 3.43%, pH value is 6-6.8, meets the standard of organic fertilizer, directly can be used as organic fertilizer application.
Embodiment 5
The embodiment of the present invention 5 provides a kind of preparation method of biological organic fertilizer, and it comprises the following steps:
Fermentation substrate is provided: provide water content be 67% chicken manure just organic waste, as fermentation substrate, wherein, described fermentation substrate is made up of the organic waste of following mass percent: feces of livestock and poultry 45%, stalk 27.5%, edible fungi leftovers 27.5%, and described stalk and edible fungi leftovers are the particle of 3-5mm;
Stack fermentation substrate: the ratio adding the fermented liq microbiobacterial agent of 1L according to every 1000kg fermentation substrate, after described fermentation substrate is mixed with described fermented liq microbiobacterial agent, again with 4 mixings of fertilizer turnover throwing machine, and stack into the rectangle fermentation substrate heap of high 0.5-0.6m;
Fermenting substrate: described rectangle fermentation substrate piles up 48-52 DEG C and ferments, the 10 days secondary fermentation substrates that ferment are loose, terminate fermentation, obtain biological organic fertilizer during odorless.
Wherein, the described fermented liq microbiobacterial agent adopted in the present embodiment comprises the component of following percent by volume: subtilis bacterium liquid 50%, Bacillus licheniformis liquid 12%, S. cervisiae liquid 16%, lactobacillus bacterium liquid 8% and black-koji mould liquid 14%, wherein, the concentration of described subtilis bacterium liquid, described Bacillus licheniformis liquid, described S. cervisiae liquid, described lactobacillus bacterium liquid and described black-koji mould liquid is 1,100,000,000 cfu/ml.
The preparation method of the above-mentioned fermented liq microbiobacterial agent that the present embodiment uses, the preparation method of the fermented liq microbiobacterial agent used with embodiment 3 is substantially identical, difference is: in the step of described enlarged culturing, five kinds of micro-raw bacterium bacterium liquid of the described concentration that the present embodiment finally obtained uses; Prepare in the step of microbial inoculum described, use according to the present embodiment the formula of the fermented liq microbiobacterial agent provided to mix.
The method adopting organic fertilizer NY525-2012 standard to specify is tested the described biological organic fertilizer that the preparation method provided by the present embodiment obtains, wherein, the mass percentage of the main ingredient in described biological organic fertilizer is as follows: organic 65.7%, purity nitrogen 1.67%, phosphorus (P
2o
5) 1.81%, potassium (K
2o) 2.17%, pH value is 7-8, meets the standard of organic fertilizer, directly can be used as organic fertilizer application.
Finally should be noted that: above embodiment is only in order to illustrate that technical scheme of the present invention is not intended to limit; Although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the field are to be understood that: still can modify to the specific embodiment of the present invention or carry out equivalent replacement to portion of techniques feature; And not departing from the spirit of technical solution of the present invention, it all should be encompassed in the middle of the technical scheme scope of request of the present invention protection.
Claims (10)
1. a preparation method for biological organic fertilizer, it comprises the following steps:
There is provided fermentation substrate: the organic waste providing water content between 60%-70%, as fermentation substrate;
Stack fermentation substrate: the ratio adding the fermented liq microbiobacterial agent of 0.9-1.1L according to every 1000kg fermentation substrate, described fermentation substrate and described fermented liq microbiobacterial agent are carried out Homogeneous phase mixing, stack into the rectangle fermentation substrate heap of high 0.5-0.8m, wherein, described fermented liq microbiobacterial agent comprises following component: subtilis bacterium liquid, Bacillus licheniformis liquid, S. cervisiae liquid, lactobacillus bacterium liquid and black-koji mould liquid;
Fermenting substrate: described rectangle fermentation substrate piles up 48 DEG C-68 DEG C and ferments, until the loose and odorless of described fermentation substrate.
2. the preparation method of biological organic fertilizer according to claim 1, it is characterized in that, described fermented liq microbiobacterial agent comprises the component of following percent by volume: subtilis bacterium liquid 40%-50%, Bacillus licheniformis liquid 12%-18%, S. cervisiae liquid 16%-24%, lactobacillus bacterium liquid 7%-9% and black-koji mould liquid 10%-14%, wherein, the concentration of described subtilis bacterium liquid, described Bacillus licheniformis liquid, described S. cervisiae liquid, described lactobacillus bacterium liquid and described black-koji mould liquid is 9-11 hundred million cfu/ml.
3. the preparation method of biological organic fertilizer according to claim 1 and 2, is characterized in that, the preparation method of described fermented liq microbiobacterial agent, comprises the following steps:
Activation culture: Bacillus subtilis strain, Bacillus licheniformis strain, yeast saccharomyces cerevisiae bacterial classification, Lactobacillus species, aspergillus niger strain are inoculated into respectively in corresponding substratum and carry out activation culture, obtains generation bacterial strain respectively;
Enlarged culturing: the generation bacterial strain of described subtilis and the generation bacterial strain of described Bacillus licheniformis are inoculated in broth agar culture medium respectively and cultivate, obtain the subtilis bacterium liquid described in claim 1 or 2 and Bacillus licheniformis liquid respectively; The generation bacterial strain of described yeast saccharomyces cerevisiae, the generation bacterial strain of described aspergillus niger are inoculated into respectively in PDA substratum and cultivate, obtains the S. cervisiae liquid described in claim 1 or 2 and black-koji mould liquid respectively; The generation inoculation of described lactobacillus is cultivated in MRS substratum, obtains the lactobacillus bacterium liquid described in claim 1 or 2.
4. prepare microbial inoculum: according to the component described in claim 1 or 2, described subtilis bacterium liquid, described Bacillus licheniformis liquid, described S. cervisiae liquid, described lactobacillus bacterium liquid and described black-koji mould liquid are mixed, obtains described fermented liq microbiobacterial agent.
5. the preparation method of biological organic fertilizer according to claim 3, it is characterized in that, the step of described activation culture comprises: adopt broth agar culture medium to carry out activation culture to described Bacillus subtilis strain and described Bacillus licheniformis strain respectively, obtain the generation bacterial strain of described subtilis and the generation bacterial strain of described Bacillus licheniformis respectively; Adopt PDA substratum to carry out activation culture to described yeast saccharomyces cerevisiae bacterial classification and described aspergillus niger strain respectively, obtain the generation bacterial strain of described yeast saccharomyces cerevisiae and the generation bacterial strain of described aspergillus niger respectively; Adopt MRS substratum to carry out activation culture to described Lactobacillus species, obtain described lactobacillus generation bacterial strain.
6. the preparation method of biological organic fertilizer according to claim 4, is characterized in that, the culture temperature in described activation culture step is 29-31 DEG C, and the activation culture time is 20-28 hour.
7. the preparation method of biological organic fertilizer according to claim 4, is characterized in that, described in the culture temperature that spreads cultivation in culturing step be 27-29 DEG C, the incubation time that spreads cultivation is 40-56 hour.
8. the preparation method of biological organic fertilizer according to claim 1 and 2, is characterized in that, described organic waste is one or more in feces of livestock and poultry, stalk, edible fungi leftovers.
9. the preparation method of biological organic fertilizer according to claim 1 and 2, it is characterized in that, described fermentation substrate comprises the organic waste of following mass percent: feces of livestock and poultry 35%-45%, stalk 27.5%-32.5%, edible fungi leftovers 27.5%-32.5%, and described stalk and edible fungi leftovers are the particle of 3-5mm.
10. the preparation method of biological organic fertilizer according to claim 1 and 2, is characterized in that, in the step of described fermenting substrate, the fermentation time of described rectangle fermentation substrate heap is 8-10 days.
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