CN113511937A - Compound bacillus subtilis bio-organic fertilizer and preparation method thereof - Google Patents
Compound bacillus subtilis bio-organic fertilizer and preparation method thereof Download PDFInfo
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- CN113511937A CN113511937A CN202110574552.3A CN202110574552A CN113511937A CN 113511937 A CN113511937 A CN 113511937A CN 202110574552 A CN202110574552 A CN 202110574552A CN 113511937 A CN113511937 A CN 113511937A
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Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a technical scheme for realizing the aim, which comprises the following steps: a compound bacillus subtilis biological organic fertilizer comprises organic matters and compound floras, wherein the compound floras comprise at least two of bacillus subtilis, saccharomycetes and actinomycetes, and the organic matters comprise one or more of crop straws, organic wastes, animal wastes and agricultural product waste gas; the preparation method of the compound bacillus subtilis bio-organic fertilizer comprises the following specific steps: s1, preparing a bacterial liquid; s2, strain culture; s3, checking the number of strains; s4, organic matter treatment; s5, mixing the complex flora and the organic matters; s6, fermenting and decomposing. The invention has high active bacteria quantity, and the method is simple and easy to operate.
Description
Technical Field
The invention relates to the technical field of biological organic fertilizers, in particular to a compound bacillus subtilis biological organic fertilizer and a preparation method thereof.
Background
The bio-organic fertilizer is a fertilizer obtained by mixing a specific microorganism with an organic material and decomposing the mixture, and is widely used because of its high efficiency and safety and harmlessness to the environment.
Some biological organic fertilizers have low active bacteria quantity, and the preparation method is complicated.
Disclosure of Invention
The invention aims to provide a compound bacillus subtilis bio-organic fertilizer and a preparation method thereof, and aims to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the compound bacillus subtilis biological organic fertilizer comprises organic matters and compound floras, wherein the compound floras comprise at least two of bacillus subtilis, saccharomycetes and actinomycetes, and the organic matters comprise one or more of crop straws, organic wastes, animal wastes and agricultural product waste gas.
Preferably, the composite flora comprises bacillus subtilis and saccharomycetes, and the mass mixing ratio of the bacillus subtilis to the saccharomycetes is 2: 1.
Preferably, the composite flora comprises bacillus subtilis and actinomycetes, and the mass mixing ratio of the bacillus subtilis to the saccharomycetes is 2: 1.
Preferably, the composite flora comprises bacillus subtilis, yeast and actinomycetes, and the bacillus subtilis, the yeast and the actinomycetes are installed in the following mass mixing ratio: 2: 1.
A preparation method of a compound bacillus subtilis bio-organic fertilizer comprises the following specific steps:
s1, preparing a bacterial liquid: injecting the composite flora into a culture dish, and adding culture solution at the same time;
s2, strain culture: culturing under aseptic condition at 25-38 deg.C under pH of 6.9-7.5;
s3, checking the number of strains: checking the number of strains to keep the number of effective viable strains at 130-250 hundred million/g;
s4, organic matter treatment: crushing and drying the organic matters for later use;
s5, mixing the complex flora and the organic matter: adding the composite flora into organic matters, adding auxiliary materials, keeping the pH value at 7.0-7.5, and controlling the water content at 50% -60%;
s6, fermenting and decomposing: and (4) piling and covering the mixture obtained in the step S5, keeping the environmental temperature not lower than 15 ℃, turning and cooling the mixture when the temperature of the mixture is raised to 60 ℃ to ensure that the pile temperature is not higher than 65 ℃, keeping the water content of the mixture to be 45-55 wt%, and fermenting for 7-9 days to finally obtain the organic fertilizer.
S7, packaging the organic fertilizer.
Preferably, the components of the culture solution in the step S1 include 1-2 parts of glucose, 0.05-0.06 part of magnesium sulfate, 2-3 parts of agar, 1-2 parts of peptone and 100 parts of purified water according to the mass ratio.
Preferably, the auxiliary material in step S5 includes at least one of rice bran and sawdust, and the ratio of the auxiliary material to the organic material is 1: 5-1: 3.
Preferably, in step S6, the height of the mixture is not less than 1m, and the volume is not less than 4 square.
Compared with the prior art, the invention has the beneficial effects that:
1. the composite organic fertilizer combines saccharomycetes and/or actinomycetes and the like, does not antagonize each other, has synergistic effect, not only has strong decomposition effect on organic materials, but also breeds a large number of functional bacteria and generates various specific metabolites in the fermentation process, thereby stimulating the growth and development of crops and improving the disease resistance, drought resistance and cold resistance of the crops;
2. the invention has high effective viable count and good use effect;
3. the preparation method is simple to operate, and can ensure the effective bacteria quantity.
Drawings
FIG. 1 is a cross-sectional view of the overall structure of a composite bacillus subtilis bio-organic fertilizer and a preparation method thereof.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Referring to fig. 1, the present invention provides a technical solution: a composite bacillus subtilis biological organic fertilizer comprises organic matters and composite flora, wherein the composite flora comprises at least two of bacillus subtilis, saccharomycetes and actinomycetes, and the organic matters comprise one or more of crop straws, organic wastes, animal wastes and agricultural product waste gas.
The composite flora comprises bacillus subtilis and actinomycetes, and the mass mixing ratio of the bacillus subtilis to the saccharomycetes is 2: 1.
A preparation method of a compound bacillus subtilis bio-organic fertilizer comprises the following specific steps:
s1, preparing a bacterial liquid: injecting the composite flora into a culture dish, and adding culture solution at the same time;
s2, strain culture: culturing under aseptic condition at 30 deg.C under pH of 7.2;
s3, checking the number of strains: checking the number of strains to keep the number of effective viable strains at 200 hundred million/g;
s4, organic matter treatment: crushing and drying the organic matters for later use;
s5, mixing the complex flora and the organic matter: adding the composite flora into organic matters, adding auxiliary materials, keeping the pH value at 7.2, and controlling the water content at 55%;
s6, fermenting and decomposing: and (4) piling and covering the mixture obtained in the step S5, keeping the environmental temperature not lower than 15 ℃, turning and cooling the mixture when the temperature of the mixture is raised to 60 ℃ to ensure that the pile temperature is not higher than 65 ℃, keeping the water content of the mixture to be 50 wt%, and fermenting for 7 days to finally obtain the organic fertilizer.
S7, packaging the organic fertilizer.
The auxiliary materials in the step S5 comprise at least one of rice bran and sawdust, and the ratio of the auxiliary materials to the organic matters is 1: 3.
In step S6, the height of the piled mixture is not less than 1m, and the volume is not less than 4 square.
The components of the culture solution in step S1 include, by mass, 1 part of glucose, 0.05 part of magnesium sulfate, 2 parts of agar, 1 part of peptone, and 100 parts of purified water.
Example 2
Referring to fig. 1, the present invention provides a technical solution: a composite bacillus subtilis biological organic fertilizer comprises organic matters and composite flora, wherein the composite flora comprises at least two of bacillus subtilis, saccharomycetes and actinomycetes, and the organic matters comprise one or more of crop straws, organic wastes, animal wastes and agricultural product waste gas.
The composite flora comprises bacillus subtilis and saccharomycetes, wherein the mass mixing ratio of the bacillus subtilis to the saccharomycetes is 2: 1.
A preparation method of a compound bacillus subtilis bio-organic fertilizer comprises the following specific steps:
s1, preparing a bacterial liquid: injecting the composite flora into a culture dish, and adding culture solution at the same time;
s2, strain culture: culturing under sterile condition at 38 deg.C under pH of 7.5;
s3, checking the number of strains: checking the number of strains to keep the number of effective viable strains at 250 hundred million/g;
s4, organic matter treatment: crushing and drying the organic matters for later use;
s5, mixing the complex flora and the organic matter: adding the composite flora into organic matters, adding auxiliary materials, keeping the pH value at 7.5, and controlling the water content at 60%;
s6, fermenting and decomposing: stacking and covering the mixture obtained in the step S5, keeping the environmental temperature not lower than 15 ℃, turning and cooling the mixture when the temperature of the mixture is raised to 60 ℃ to ensure that the temperature of the stack is not higher than 65 ℃, keeping the water content of the mixture to be 55 wt%, and fermenting for 7 days to finally obtain the organic fertilizer;
s7, packaging the organic fertilizer.
The auxiliary materials in the step S5 comprise at least one of rice bran and sawdust, and the ratio of the auxiliary materials to the organic matters is 1: 3.
In step S6, the height of the piled mixture is not less than 1m, and the volume is not less than 4 square.
The components of the culture solution in step S1 include, by mass, 2 parts of glucose, 0.06 part of magnesium sulfate, 3 parts of agar, 2 parts of peptone, and 100 parts of purified water.
Example 3
Referring to fig. 1, the present invention provides a technical solution: a composite bacillus subtilis biological organic fertilizer comprises organic matters and composite flora, wherein the composite flora comprises at least two of bacillus subtilis, saccharomycetes and actinomycetes, and the organic matters comprise one or more of crop straws, organic wastes, animal wastes and agricultural product waste gas.
The composite flora comprises bacillus subtilis, saccharomycetes and actinomycetes, and the mixing ratio of the bacillus subtilis, the saccharomycetes and the actinomycetes in the mounting mass is as follows: 2: 1.
A preparation method of a compound bacillus subtilis bio-organic fertilizer comprises the following specific steps:
s1, preparing a bacterial liquid: injecting the composite flora into a culture dish, and adding culture solution at the same time;
s2, strain culture: culturing under sterile condition at 37 deg.C under pH of 7.2;
s3, checking the number of strains: checking the number of strains to keep the number of effective viable strains at 250 hundred million/g;
s4, organic matter treatment: crushing and drying the organic matters for later use;
s5, mixing the complex flora and the organic matter: adding the composite flora into organic matters, adding auxiliary materials, keeping the pH value at 7.2, and controlling the water content at 55%;
s6, fermenting and decomposing: stacking and covering the mixture obtained in the step S5, keeping the environmental temperature not lower than 15 ℃, turning and cooling the mixture when the temperature of the mixture is raised to 60 ℃ to ensure that the temperature of the stack is not higher than 65 ℃, keeping the water content of the mixture to be 50 wt%, and fermenting for 9 days to finally obtain the organic fertilizer;
s7, packaging the organic fertilizer.
The auxiliary materials in the step S5 comprise at least one of rice bran and sawdust, and the ratio of the auxiliary materials to the organic matters is 1: 3.
In step S6, the height of the piled mixture is not less than 1m, and the volume is not less than 4 square.
The components of the culture solution in step S1 include, by mass, 1 part of glucose, 0.05 part of magnesium sulfate, 2 parts of agar, 1 part of peptone, and 100 parts of purified water.
Example 4
Referring to fig. 1, the present invention provides a technical solution: a composite bacillus subtilis biological organic fertilizer comprises organic matters and composite flora, wherein the composite flora comprises at least two of bacillus subtilis, saccharomycetes and actinomycetes, and the organic matters comprise one or more of crop straws, organic wastes, animal wastes and agricultural product waste gas.
The composite flora comprises bacillus subtilis, saccharomycetes and actinomycetes, and the mixing ratio of the bacillus subtilis, the saccharomycetes and the actinomycetes in the mounting mass is as follows: 2: 1.
A preparation method of a compound bacillus subtilis bio-organic fertilizer comprises the following specific steps:
s1, preparing a bacterial liquid: injecting the composite flora into a culture dish, and adding culture solution at the same time;
s2, strain culture: culturing under sterile condition at 37 deg.C under pH of 7.2;
s3, checking the number of strains: checking the number of strains to keep the number of effective viable strains at 250 hundred million/g;
s4, organic matter treatment: crushing and drying the organic matters for later use;
s5, mixing the complex flora and the organic matter: adding the composite flora into organic matters, adding auxiliary materials, keeping the pH value at 7.2, and controlling the water content at 55%;
s6, fermenting and decomposing: stacking and covering the mixture obtained in the step S5, keeping the environmental temperature not lower than 15 ℃, turning and cooling the mixture when the temperature of the mixture is raised to 60 ℃ to ensure that the temperature of the stack is not higher than 65 ℃, keeping the water content of the mixture to be 50 wt%, and fermenting for 9 days to finally obtain the organic fertilizer;
s7, packaging the organic fertilizer.
The auxiliary materials in the step S5 comprise at least one of rice bran and sawdust, and the ratio of the auxiliary materials to the organic matters is 1: 5.
In step S6, the height of the piled mixture is not less than 1m, and the volume is not less than 4 square.
The components of the culture solution in step S1 include, by mass, 1 part of glucose, 0.05 part of magnesium sulfate, 2 parts of agar, 1 part of peptone, and 100 parts of purified water.
Example 5
Referring to fig. 1, the present invention provides a technical solution: a composite bacillus subtilis biological organic fertilizer comprises organic matters and composite flora, wherein the composite flora comprises at least two of bacillus subtilis, saccharomycetes and actinomycetes, and the organic matters comprise one or more of crop straws, organic wastes, animal wastes and agricultural product waste gas.
The composite flora comprises bacillus subtilis and saccharomycetes, wherein the mass mixing ratio of the bacillus subtilis to the saccharomycetes is 2: 1.
A preparation method of a compound bacillus subtilis bio-organic fertilizer comprises the following specific steps:
s1, preparing a bacterial liquid: injecting the composite flora into a culture dish, and adding culture solution at the same time;
s2, strain culture: culturing under sterile condition at 38 deg.C under pH of 7.5;
s3, checking the number of strains: checking the number of strains to keep the number of effective viable strains at 250 hundred million/g;
s4, organic matter treatment: crushing and drying the organic matters for later use;
s5, mixing the complex flora and the organic matter: adding the composite flora into organic matters, adding auxiliary materials, keeping the pH value at 7.5, and controlling the water content at 60%;
s6, fermenting and decomposing: stacking and covering the mixture obtained in the step S5, keeping the environmental temperature not lower than 15 ℃, turning and cooling the mixture when the temperature of the mixture is raised to 60 ℃ to ensure that the temperature of the stack is not higher than 65 ℃, keeping the water content of the mixture to be 55 wt%, and fermenting for 7 days to finally obtain the organic fertilizer;
s7, packaging the organic fertilizer.
The auxiliary materials in the step S5 comprise at least one of rice bran and sawdust, and the ratio of the auxiliary materials to the organic matters is 1: 5.
In step S6, the height of the piled mixture is not less than 1m, and the volume is not less than 4 square.
The components of the culture solution in step S1 include, by mass, 2 parts of glucose, 0.06 part of magnesium sulfate, 3 parts of agar, 2 parts of peptone, and 100 parts of purified water.
Active substances such as subtilin, polymyxin, nystatin, gramicidin and the like generated in the growth process of bacillus subtilis have obvious inhibition effect on pathogenic bacteria or conditional pathogenic bacteria of endogenous infection, the bacillus subtilis successfully colonizes to the rhizosphere, the body surface or the body of a plant, competes with the pathogenic bacteria for nutrition around the plant, secretes antibacterial substances to inhibit the growth of the pathogenic bacteria, and induces a plant defense system to resist the invasion of the pathogenic bacteria, thereby achieving the purpose of biocontrol. The bacillus subtilis can mainly inhibit various plant diseases caused by plant pathogenic bacteria such as filamentous fungi. Such as rice sheath blight disease, rice blast, wheat sheath blight disease, and bean root rot in food crops. Tomato leaf disease, fusarium wilt, cucumber fusarium wilt, downy mildew, eggplant gray mold and powdery mildew, pepper phytophthora blight and the like in vegetable diseases.
Simultaneously, saccharomycetes and/or actinomycetes and the like are compounded, mutual antagonism and synergistic effect are realized, strong decomposition effect is realized on organic materials, a large number of functional bacteria are propagated in the fermentation process, and various specific metabolites are generated, so that the growth and development of crops are stimulated, the disease resistance, drought resistance and cold resistance of the crops are improved, after the functional bacteria enter the soil, nitrogen fixation, phosphorus and potassium dissolution can be realized, the soil nutrients are increased, the soil structure is improved, the fertilizer utilization rate is improved, and the composite fertilizer is non-toxic, harmless and pollution-free.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A compound bacillus subtilis bio-organic fertilizer comprises organic matters and compound floras, and is characterized in that: the composite flora comprises at least two of bacillus subtilis, yeast and actinomycetes, and the organic matter comprises one or more of crop straws, organic wastes, animal wastes and agricultural product waste gas.
2. The compound bacillus subtilis bio-organic fertilizer of claim 1, which is characterized in that: the composite flora comprises bacillus subtilis and saccharomycetes, wherein the mass mixing ratio of the bacillus subtilis to the saccharomycetes is 2: 1.
3. The compound bacillus subtilis bio-organic fertilizer of claim 1, which is characterized in that: the composite flora comprises bacillus subtilis and actinomycetes, and the mass mixing ratio of the bacillus subtilis to the saccharomycetes is 2: 1.
4. The compound bacillus subtilis bio-organic fertilizer of claim 1, which is characterized in that: the composite flora comprises bacillus subtilis, saccharomycetes and actinomycetes, and the bacillus subtilis, the saccharomycetes and the actinomycetes are installed in a mass mixing ratio of: 2: 1.
5. A preparation method of a compound bacillus subtilis bio-organic fertilizer is characterized by comprising the following specific steps:
s1, preparing a bacterial liquid: injecting the composite flora into a culture dish, and adding culture solution at the same time;
s2, strain culture: culturing under aseptic condition at 25-38 deg.C under pH of 6.9-7.5;
s3, checking the number of strains: checking the number of strains to keep the number of effective viable strains at 130-250 hundred million/g;
s4, organic matter treatment: crushing and drying the organic matters for later use;
s5, mixing the complex flora and the organic matter: adding the composite flora into organic matters, adding auxiliary materials, keeping the pH value at 7.0-7.5, and controlling the water content at 50% -60%;
s6, fermenting and decomposing: stacking and covering the mixture obtained in the step S5, keeping the environmental temperature not lower than 15 ℃, turning and cooling the mixture when the temperature of the mixture is raised to 60 ℃ to ensure that the temperature of the stack is not higher than 65 ℃, keeping the water content of the mixture to be 45-55 wt%, and fermenting for 7-9 days to finally obtain the organic fertilizer;
s7, packaging the organic fertilizer.
6. The compound bacillus subtilis bio-organic fertilizer and the preparation method thereof as claimed in claim 5, wherein the compound bacillus subtilis bio-organic fertilizer is characterized in that: the auxiliary materials in the step S5 comprise at least one of rice bran and sawdust, and the ratio of the auxiliary materials to the organic matters is 1: 5-1: 3.
7. The compound bacillus subtilis bio-organic fertilizer and the preparation method thereof as claimed in claim 5, wherein the compound bacillus subtilis bio-organic fertilizer is characterized in that: in the step S6, the height of the piled mixture is not less than 1m, and the volume is not less than 4 square.
8. The compound bacillus subtilis bio-organic fertilizer and the preparation method thereof as claimed in claim 5, wherein the compound bacillus subtilis bio-organic fertilizer is characterized in that: the components of the culture solution in the step S1 comprise 1-2 parts of glucose, 0.05-0.06 part of magnesium sulfate, 2-3 parts of agar, 1-2 parts of peptone and 100 parts of purified water according to the mass ratio.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101508601A (en) * | 2009-03-31 | 2009-08-19 | 孙明坤 | Microorganism organic fertilizer and method of preparing the same |
| CN102838390A (en) * | 2012-08-31 | 2012-12-26 | 成都市家家美食品有限公司 | Ecological organic fertilizer |
| CN105418172A (en) * | 2016-01-11 | 2016-03-23 | 鹤壁市人元生物技术发展有限公司 | Preparation method of biological organic fertilizer |
| CN106518284A (en) * | 2016-09-30 | 2017-03-22 | 广西汇智生产力促进中心有限公司 | Bio-organic fertilizer for dragon fruit planting and preparing method thereof |
| CN107244950A (en) * | 2017-06-27 | 2017-10-13 | 梁平县丰疆生物科技有限公司 | Cattle manure method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101508601A (en) * | 2009-03-31 | 2009-08-19 | 孙明坤 | Microorganism organic fertilizer and method of preparing the same |
| CN102838390A (en) * | 2012-08-31 | 2012-12-26 | 成都市家家美食品有限公司 | Ecological organic fertilizer |
| CN105418172A (en) * | 2016-01-11 | 2016-03-23 | 鹤壁市人元生物技术发展有限公司 | Preparation method of biological organic fertilizer |
| CN106518284A (en) * | 2016-09-30 | 2017-03-22 | 广西汇智生产力促进中心有限公司 | Bio-organic fertilizer for dragon fruit planting and preparing method thereof |
| CN107244950A (en) * | 2017-06-27 | 2017-10-13 | 梁平县丰疆生物科技有限公司 | Cattle manure method |
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