CN107244950A - Cattle manure method - Google Patents

Cattle manure method Download PDF

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Publication number
CN107244950A
CN107244950A CN201710497919.XA CN201710497919A CN107244950A CN 107244950 A CN107244950 A CN 107244950A CN 201710497919 A CN201710497919 A CN 201710497919A CN 107244950 A CN107244950 A CN 107244950A
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China
Prior art keywords
mixture
culture medium
fermentation
parts
hours
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Pending
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CN201710497919.XA
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Chinese (zh)
Inventor
边海平
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LIANGPING COUNTY FENGJIANG BIOLOGICAL TECHNOLOGY Co Ltd
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LIANGPING COUNTY FENGJIANG BIOLOGICAL TECHNOLOGY Co Ltd
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Priority to CN201710497919.XA priority Critical patent/CN107244950A/en
Publication of CN107244950A publication Critical patent/CN107244950A/en
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00—Fertilisers from human or animal excrements, e.g. manure
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The present invention relates to organic fertilizer manufacture technology field, a kind of cattle manure method is disclosed, including：S1：2~4 parts of bacillus subtilises, 1~3 part of bacillus licheniformis, 1~2 part of Penicillium notatum, 1~5 part of actinomyces are taken by mass fraction, and are activated 22~26 hours；S2：Bacillus subtilis after activation, bacillus licheniformis and Penicillium notatum are put into the first culture medium and fermented 18~20 hours, the actinomyces after activation are put into the second culture medium and fermented 18~20 hours, S3：Mix the first culture medium and the second culture medium each ferment after microbial inoculum, take 70~75 parts of cow dungs, 20~25 parts of auxiliary materials and 0.4~0.8 part of microbial inoculum formation mixture by mass fraction, bar heap formula carried out to mixture and fermented；S4：When fermentation temperature reaches 60 DEG C~70 DEG C, the mixture is transferred to fermentation tank, the mixture is carried out in fermentation tank to turn over throwing fermentation, until the moisture of mixture is less than 30%.The cattle manure method quick heating of the present invention, degree of becoming thoroughly decomposed are high, and fermentation period is shorter.

Description

Cattle manure method
Technical field
The present invention relates to organic fertilizer manufacture technology field, more particularly to a kind of cattle manure method.
Background technology
Have become the trend of agricultural production, organic fertilizer and chemical fertilizer phase as the fertilizer of crops using organic fertilizer at present Than, the nutrient that organic fertilizer contains is relatively more, can improved soil prevent hardenedization.Product quality is improved, Resistant is improved Ability, Optimum utilization rate；Compared with common farm manure, organic fertilizer is destitute of smell, easy to use and uniform.And organic fertilizer With the addition of beneficial bacterium can reduce disease occur and also nutrient content is also higher.
Existing organic fertilizer using fowl and animal excrements such as chicken manure and pig manures of fermentation using being made up mostly, and cow dung is relative to it His fowl and animal excrement, crude fiber content is higher so that have that heating is slow, fiber testing is low, journey of becoming thoroughly decomposed during organic fertilizer fermentation Spend the problem of not high and fermentation period is long.
The content of the invention
The present invention proposes a kind of cattle manure method, solves that cattle manure heating in the prior art is slow, fiber testing Low, degree of becoming thoroughly decomposed is not high and the problem of long fermentation period.
A kind of cattle manure method of the present invention, including step：
S1：By mass fraction take 2~4 parts of bacillus subtilises, 1~3 part of bacillus licheniformis, 1~2 portion of Penicillium notatum, 1~ 5 parts of actinomyces, and activate 22~26 hours；
S2：Bacillus subtilis after activation, bacillus licheniformis and Penicillium notatum are put into the first culture medium and ferment 18 ~20 hours, the actinomyces after activation are put into the second culture medium and fermented 18~20 hours, first culture medium presses quality Percentage includes：CMC-Na0.4~0.6%, (NH4)2SO40.2~0.3%, peptone 0.2~0.4%, CaCl20.01~ 0.03%, KH2PO40.2~0.4%, remaining is water；Second culture medium includes by mass percentage：Glucose 0.5~ 0.8%, bean powder 0.2~0.5%, FeCl30.02~0.04%, yeast extract 0.1~0.3%, KH2PO40.02~0.04%, MgSO40.03~0.06%, remaining is water；
S3：Mix the first culture medium and the second culture medium each ferment after microbial inoculum, take 70~75 portions of oxen by mass fraction Excrement, 20~25 parts of auxiliary materials and 0.4~0.8 part of microbial inoculum formation mixture, carry out bar heap formula to mixture and ferment, the accessory package Include：At least one of waste mushroom packet, vinasse, sawdust, wood shavings and chaff powder；
S4：When fermentation temperature reaches 60 DEG C~70 DEG C, the mixture is transferred to fermentation tank, to institute in fermentation tank State mixture to carry out turning over throwing fermentation, until the moisture of mixture is less than 30%.
Wherein, the quality parts ratio of the bacillus subtilis, bacillus licheniformis, Penicillium notatum and actinomyces is：2:2: 2:5。
Wherein, the step S4 includes：
The mixture is transferred to after fermentation tank, cast using turning over for mixture is evenly laid out in fermentation tank；
After fermentation 4~6 hours, turning and throwing once, to distribute moisture, until the moisture of mixture is less than 30%.
Wherein, cow dung is air-dried before mixing, and is crushed, and suitable quantity of water mixing is added during mixing, makes containing for mixture Water is 60%~70%.
A variety of strains are mixed in the cattle manure method of the present invention, to form different cellulose degrading enzymes, energy Crude fibre in enough quick, cow dungs of thoroughly degrading, quick heating, degree of becoming thoroughly decomposed are high during the fermentation for cow dung, and fermentation period It is shorter.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing There is the accompanying drawing used required in technology description to be briefly described, it should be apparent that, drawings in the following description are only this Some embodiments of invention, for those of ordinary skill in the art, without having to pay creative labor, may be used also To obtain other accompanying drawings according to these accompanying drawings.
Fig. 1 is a kind of cattle manure method flow diagram of the invention.
Embodiment
The present invention cattle manure method as shown in figure 1, including：
Step S1,2~4 parts of bacillus subtilises, 1~3 part of bacillus licheniformis, 1~2 part of mould are taken by mass fraction Bacterium, 1~5 part of actinomyces, and activate 22~26 hours；
Step S2, the bacillus subtilis after activation, bacillus licheniformis and Penicillium notatum are put into the first culture medium and sent out Ferment 18~20 hours, the actinomyces after activation are put into the second culture medium and fermented 18~20 hours, first culture medium is pressed Mass percent includes：0.4~0.6% CMC-Na, (NH4)2SO40.2~0.3%, peptone 0.2~0.4%, CaCl20.01~0.03%, KH2PO40.2~0.4%, remaining is water；Second culture medium includes by mass percentage：Portugal Grape sugar 0.5~0.8%, bean powder 0.2~0.5%, FeCl30.02~0.04%, yeast extract 0.1~0.3%, KH2PO40.02~ 0.04%, MgSO40.03~0.06%, remaining is water；
Step S3, mixing the first culture medium and the second culture medium each ferment after microbial inoculum, take 70~75 by mass fraction Part cow dung, 20~25 parts of auxiliary materials and 0.4~0.8 part of microbial inoculum formation mixture, carry out bar heap formula to mixture and ferment, the auxiliary material Including：At least one of waste mushroom packet, vinasse, sawdust, wood shavings and chaff powder；
Step S4, when fermentation temperature reaches 60 DEG C~70 DEG C, is transferred to fermentation tank, in fermentation tank by the mixture The mixture is carried out to turn over throwing fermentation, until the moisture of mixture is less than 30%.
Single cellulose-degrading bacteria can not produce the cellulase of 3 types, cattle manure of the invention simultaneously mostly Bacillus subtilis, bacillus licheniformis, Penicillium notatum and actinomyces are mixed in method, to produce the fiber of different ratio Plain enzyme (including：Endoglucanase, exoglucanase, beta-glucosidase) common degradation of fibers material.Meanwhile, in many bacterium In the environment that strain coexists, the feedback inhibition of product can also be alleviated, so as to improve the conversion ratio of cellulose.And Penicillium notatum exists Mycelia can be produced in growth course, mycelia has very strong penetration capacity, can penetrate the obstruction of Plant cuticle, tightly depend on and It is interspersed on fibrous matter, the contact area of increase digestive enzyme and fibrous matter, so as to accelerate degradation rate.
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made Embodiment, belongs to the scope of protection of the invention.
Embodiment 1
The cattle manure method of the present embodiment, including：
Step S1,2 parts of bacillus subtilises, 2 parts of bacillus licheniformis, 2 parts of Penicillium notatums, 5 parts of unwrapping wire are taken by mass fraction Bacterium, and activate 26 hours.In follow-up step S2, cultured bacterium solution is combined orthogonal test, cellulase is detected Living, the quality parts ratio in bacillus subtilis, bacillus licheniformis, Penicillium notatum and actinomyces that experiment is surveyed is 2:2:2:When 5, Cellulase activity is maximum.
Step S2, the bacillus subtilis after activation, bacillus licheniformis and Penicillium notatum are put into the first culture medium and sent out Ferment 20 hours, the actinomyces after activation are put into the second culture medium and fermented 20 hours, and first culture medium presses quality percentage Than including：CMC-Na0.6%, (NH4)2SO40.3%, peptone 0.4%, CaCl20.03%, KH2PO40.4%, remaining is water； Second culture medium includes by mass percentage：Glucose 0.8%, bean powder 0.5%, FeCl30.04%, yeast extract 0.3%, KH2PO40.04%, MgSO40.06%, remaining is water.
Step S3, mixing the first culture medium and the second culture medium each ferment after microbial inoculum, take 75 portions of oxen by mass fraction Excrement, 25 parts of auxiliary materials and 0.8 part of microbial inoculum formation mixture, carry out bar heap formula to mixture and ferment, the auxiliary material includes：Waste mushroom packet, At least one of vinasse, sawdust, wood shavings and chaff powder.Waste mushroom packet, vinasse, sawdust, wood shavings and chaff powder are discarded in technique Thing, can reduce the cost of organic fertilizer making.
Step S4, when fermentation temperature reaches 70 DEG C, is transferred to fermentation tank, to described in fermentation tank by the mixture Mixture carries out turning over throwing fermentation, until the moisture of mixture is less than 30%.Fermentation temperature can be killed when reaching more than 60 DEG C Worm's ovum and weed seed in dead excrement, now the moisture of mixture is about 50%~60%, in order to ensure in packaging After be easy to preserve not breed bacteria, therefore mixture be transferred to fermentation tank turn over throwings so that moisture evaporation is to less than 30%
Step S4 includes：The mixture is transferred to after fermentation tank, using turn over cast it is standby by mixture it is evenly laid out In fermentation tank；After fermentation 4 hours, turning and throwing once, to distribute moisture, until the moisture of mixture is less than 30%.
The present embodiment uses fermenting twice, for the first time using the fermentation of bar heap formula, mixture can Rapid Fermentation to becoming thoroughly decomposed or Close to the degree become thoroughly decomposed, when being fermented in fermentation tank for second, throwing is turned over alternately so that mixture further becomes thoroughly decomposed, and Moisture is low, it is easy to packaging and storing, and relative to the existing mode once become thoroughly decomposed, improves production efficiency.
In the present embodiment, cow dung is air-dried before mixing, and is crushed, and suitable quantity of water mixing is added during mixing, makes mixing The water content of thing is 70%.Air-dry crude fibre after crushing and, into graininess, increase the contact area with microbial inoculum, so as to accelerate fermentation Speed.
Embodiment 2
The cattle manure method of the present embodiment, including：
Step S1,2 parts of bacillus subtilises, 2 parts of bacillus licheniformis, 2 parts of Penicillium notatums, 5 parts of unwrapping wire are taken by mass fraction Bacterium, and activate 26 hours.In follow-up step S2, cultured bacterium solution is combined orthogonal test, cellulase is detected Living, the quality parts ratio in bacillus subtilis, bacillus licheniformis, Penicillium notatum and actinomyces that experiment is surveyed is 2:2:2:When 5, Cellulase activity is maximum.
Step S2, the bacillus subtilis after activation, bacillus licheniformis and Penicillium notatum are put into the first culture medium and sent out Ferment 18 hours, the actinomyces after activation are put into the second culture medium and fermented 18 hours, and first culture medium presses quality percentage Than including：CMC-Na0.4%, (NH4)2SO40.2%, peptone 0.2%, CaCl20.01%, KH2PO40.2%, remaining is water； Second culture medium includes by mass percentage：Glucose 0.5%, bean powder 0.2%, FeCl30.02%, yeast extract 0.1%, KH2PO40.02%, MgSO40.03%, remaining is water.
Step S3, mixing the first culture medium and the second culture medium each ferment after microbial inoculum, take 70 portions of oxen by mass fraction Excrement, 20 parts of auxiliary materials and 0.4 part of microbial inoculum formation mixture, carry out bar heap formula to mixture and ferment, the auxiliary material includes：Waste mushroom packet, At least one of vinasse, sawdust, wood shavings and chaff powder.Waste mushroom packet, vinasse, sawdust, wood shavings and chaff powder are discarded in technique Thing, can reduce the cost of organic fertilizer making.
Step S4, when fermentation temperature reaches 60 DEG C, is transferred to fermentation tank, to described in fermentation tank by the mixture Mixture carries out turning over throwing fermentation, until the moisture of mixture is less than 30%.Fermentation temperature can be killed when reaching more than 60 DEG C Worm's ovum and weed seed in dead excrement, now the moisture of mixture is about 50%~60%, in order to ensure in packaging After be easy to preserve not breed bacteria, therefore mixture be transferred to fermentation tank turn over throwings so that moisture evaporation is to less than 30%
Step S4 includes：The mixture is transferred to after fermentation tank, using turn over cast it is standby by mixture it is evenly laid out In fermentation tank；After fermentation 6 hours, turning and throwing once, to distribute moisture, until the moisture of mixture is less than 30%.
The present embodiment uses fermenting twice, for the first time using the fermentation of bar heap formula, mixture can Rapid Fermentation to becoming thoroughly decomposed or Close to the degree become thoroughly decomposed, when being fermented in fermentation tank for second, throwing is turned over alternately so that mixture further becomes thoroughly decomposed, and Moisture is low, it is easy to packaging and storing, and relative to the existing mode once become thoroughly decomposed, improves production efficiency.
In the present embodiment, cow dung is air-dried before mixing, and is crushed, and suitable quantity of water mixing is added during mixing, makes mixing The water content of thing is 60%.Air-dry crude fibre after crushing and, into graininess, increase the contact area with microbial inoculum, so as to accelerate fermentation Speed.
Embodiment 3
The cattle manure method of the present embodiment, including：
Step S1,2 parts of bacillus subtilises, 2 parts of bacillus licheniformis, 2 parts of Penicillium notatums, 5 parts of unwrapping wire are taken by mass fraction Bacterium, and activate 26 hours.In follow-up step S2, cultured bacterium solution is combined orthogonal test, cellulase is detected Living, the quality parts ratio in bacillus subtilis, bacillus licheniformis, Penicillium notatum and actinomyces that experiment is surveyed is 2:2:2:When 5, Cellulase activity is maximum.
Step S2, the bacillus subtilis after activation, bacillus licheniformis and Penicillium notatum are put into the first culture medium and sent out Ferment 19 hours, the actinomyces after activation are put into the second culture medium and fermented 19 hours, and first culture medium presses quality percentage Than including：CMC-Na0.5%, (NH4)2SO40.25%, peptone 0.3%, CaCl20.02%, KH2PO40.3%, remaining is Water；Second culture medium includes by mass percentage：Glucose 0.6%, bean powder 0.4%, FeCl30.03%, yeast extract 0.2%, KH2PO40.03%, MgSO40.04%, remaining is water.
Step S3, mixing the first culture medium and the second culture medium each ferment after microbial inoculum, take 72 portions of oxen by mass fraction Excrement, 23 parts of auxiliary materials and 0.6 part of microbial inoculum formation mixture, carry out bar heap formula to mixture and ferment, the auxiliary material includes：Waste mushroom packet, At least one of vinasse, sawdust, wood shavings and chaff powder.Waste mushroom packet, vinasse, sawdust, wood shavings and chaff powder are discarded in technique Thing, can reduce the cost of organic fertilizer making.
Step S4, when fermentation temperature reaches 65 DEG C, is transferred to fermentation tank, to described in fermentation tank by the mixture Mixture carries out turning over throwing fermentation, until the moisture of mixture is less than 30%.Fermentation temperature can be killed when reaching more than 60 DEG C Worm's ovum and weed seed in dead excrement, now the moisture of mixture is about 50%~60%, in order to ensure in packaging After be easy to preserve not breed bacteria, therefore mixture be transferred to fermentation tank turn over throwings so that moisture evaporation is to less than 30%
Step S4 includes：The mixture is transferred to after fermentation tank, using turn over cast it is standby by mixture it is evenly laid out In fermentation tank；After fermentation 5 hours, turning and throwing once, to distribute moisture, until the moisture of mixture is less than 30%.
The present embodiment uses fermenting twice, for the first time using the fermentation of bar heap formula, mixture can Rapid Fermentation to becoming thoroughly decomposed or Close to the degree become thoroughly decomposed, when being fermented in fermentation tank for second, throwing is turned over alternately so that mixture further becomes thoroughly decomposed, and Moisture is low, it is easy to packaging and storing, and relative to the existing mode once become thoroughly decomposed, improves production efficiency.
In the present embodiment, cow dung is air-dried before mixing, and is crushed, and suitable quantity of water mixing is added during mixing, makes mixing The water content of thing is 65%.Air-dry crude fibre after crushing and, into graininess, increase the contact area with microbial inoculum, so as to accelerate fermentation Speed.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and Within principle, any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.

Claims (4)

1. a kind of cattle manure method, it is characterised in that including step：
S1：By mass fraction take 2~4 parts of bacillus subtilises, 1~3 part of bacillus licheniformis, 1~2 portion of Penicillium notatum, 1~5 part Actinomyces, and activate 22~26 hours；
S2：Bacillus subtilis after activation, bacillus licheniformis and Penicillium notatum are put into fermentation 18~20 in the first culture medium Hour, the actinomyces after activation are put into the second culture medium and fermented 18~20 hours, first culture medium presses quality percentage Than including：CMC-Na0.4~0.6%, (NH4)2SO40.2~0.3%, peptone 0.2~0.4%, CaCl20.01~ 0.03%, KH2PO40.2~0.4%, remaining is water；Second culture medium includes by mass percentage：Glucose 0.5~ 0.8%, bean powder 0.2~0.5%, FeCl30.02~0.04%, yeast extract 0.1~0.3%, KH2PO40.02~0.04%, MgSO40.03~0.06%, remaining is water；
S3：Mix the first culture medium and the second culture medium each ferment after microbial inoculum, take 70~75 parts of cow dungs, 20 by mass fraction ~25 parts of auxiliary materials and 0.4~0.8 part of microbial inoculum formation mixture, carry out bar heap formula to mixture and ferment, the auxiliary material includes：Useless bacterium At least one of bag, vinasse, sawdust, wood shavings and chaff powder；
S4：When fermentation temperature reaches 60 DEG C~70 DEG C, the mixture is transferred to fermentation tank, to described mixed in fermentation tank Compound carries out turning over throwing fermentation, until the moisture of mixture is less than 30%.
2. cattle manure method as claimed in claim 1, it is characterised in that the bacillus subtilis, bacillus licheniformis, The quality parts ratio of Penicillium notatum and actinomyces is：2:2:2:5.
3. cattle manure method as claimed in claim 1, it is characterised in that the step S4 includes：
The mixture is transferred to after fermentation tank, cast using turning over for mixture is evenly laid out in fermentation tank；
After fermentation 4~6 hours, turning and throwing once, to distribute moisture, until the moisture of mixture is less than 30%.
4. such as cattle manure method according to any one of claims 1 to 3, it is characterised in that cow dung is carried out before mixing Air-dry, and crush, suitable quantity of water mixing is added during mixing, the water content for making mixture is 60%~70%.
CN201710497919.XA 2017-06-27 2017-06-27 Cattle manure method Pending CN107244950A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113511937A (en) * 2021-05-25 2021-10-19 临县建兴生物科技有限公司 Compound bacillus subtilis bio-organic fertilizer and preparation method thereof

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CN105541442A (en) * 2016-02-29 2016-05-04 河北西马仑生物肥料有限公司 Continuous cropping soil conditioner and preparation method thereof
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