CN105732143A - Method for producing organic fertilizer without pile turning - Google Patents

Method for producing organic fertilizer without pile turning Download PDF

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Publication number
CN105732143A
CN105732143A CN201610129716.0A CN201610129716A CN105732143A CN 105732143 A CN105732143 A CN 105732143A CN 201610129716 A CN201610129716 A CN 201610129716A CN 105732143 A CN105732143 A CN 105732143A
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China
Prior art keywords
fermentation
prepared
fertilizer
turning
standby
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Pending
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CN201610129716.0A
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Chinese (zh)
Inventor
刘唤明
雷晓凌
邓楚津
洪鹏志
刘颖
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Guangdong Ocean University
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Guangdong Ocean University
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Priority to CN201610129716.0A priority Critical patent/CN105732143A/en
Publication of CN105732143A publication Critical patent/CN105732143A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • C05F11/08Organic fertilisers containing added bacterial cultures, mycelia or the like
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention discloses a method for producing organic fertilizer without pile turning. The method comprises the following main steps: culturing high-temperature-resistant facultative anaerobe bacillus methylotrophicus HL-4 to obtain a microbial agent for standby; mixing bacillus methylotrophicus bacteria and enzyme, and preparing a fermenting agent after uniform mixing for later use; adding the fermenting agent into organic fertilizer fermenting raw materials, and conducting pile construction and fermentation, wherein the fermentation is adopted as the natural standing fermentation, and the fermentation time is 7-40 d; drying, smashing and packaging the fermented product to prepare the finished organic fertilizer. The method for producing the organic fertilizer without pile turning is provided, so that not only can the input of pile turning equipment be reduced, but also the labor and energy costs can be reduced, and good application prospects are achieved.

Description

A kind of fertilizer manufacture method of not turning
Technical field
The present invention relates to the fertilizer manufacture method of a kind of not turning, belong to the technical field that fertilizer makes.
Background technology
Biological organic fertilizer is the organic waste produced with feces of livestock and poultry, agricultural crop straw, agricultural byproducts and food processing For raw material, it is equipped with Multifunctional fermentation inoculum agent and becomes thoroughly decomposed the organic fertilizer containing a certain amount of functional microbial of fermentation.Have Machine fertilizer contains the organic nutrients such as abundant organic matter, aminoacid, protein, also contains the inorganic nutrients such as nitrogen, phosphorus, potassium simultaneously.Raw Produce fertilizer not only can solid waste be turned waste into wealth, beneficially environmental issue, and may advantageously facilitate China's agricultural Sustainable development.
Organic fertilizer production is highly developed in China at present, and organic waste is carried out fermentation corruption by main employing aerobic microbiological Ripe.The fermenting and producing of existing fertilizer will periodically turning, produce fertilizer need put into buy turning machine.Thus, at present The production of China's fertilizer is the most all some integrated mill's centralized production.But, there is a lot of small-sized plant and vast in China The every annual meeting in rural area produces a lot of garbages, and these garbages focus on due to traffic problems inconvenience.And small-sized plant and wide Large village carries out the production of fertilizer due to the restriction of fund, the equipment that inconvenience input again is expensive.Thus, annual China still has Solid waste is wasted greatly, and this not only pollutes environment, but also can waste some organic resources.
Summary of the invention
For problem present in existing organic fertilizer production technology, the main object of the present invention is to provide one not turning Fertilizer manufacture method.
In order to solve the problems referred to above, the technical solution adopted in the present invention is as follows: the fertilizer making side of a kind of not turning Method, comprises the steps:
(1) by the high temperature resistant facultative anaerobe Methylotrophic bacillus cereus (Bacillus methylotrophicus) of slant preservation HL-4 is linked into after sterilizing in the LB fluid medium of cooling, in 55 DEG C, 150r/min cultivate 12h, be prepared as Bacillus Methylotrophicus HL-4 seed liquor is standby;
(2) by wheat bran and water be in mass ratio 1:1 ratio mixing, and mix be prepared as bran mass, bran mass in After 121 DEG C of sterilizing 30min standby;
(3) Bacillus methylotrophicus HL-4 seed liquor step (1) prepared is linked into what step (2) obtained After sterilizing in the bran mass of cooling and mix, then by the bran mass that accesses strain, to be divided in the fermentation sterilized shallow In dish, charging thickness is 4cm, and in tray, fermentation culture primary surface covers the wet gauze of sterilizing, then is placed in by tray sterilized Shelf top fermentation in solid fermentation room, control ambient temperature is at 55 DEG C, and fermentation time is 48h, and the fermentation after fermentation ends is produced It is prepared as Bacillus methylotrophicus microbial inoculum standby after thing drying and pulverizing;
(4) in Bacillus methylotrophicus microbial inoculum prepared by step (3), addition protease, cellulase, wood gather Carbohydrase, 1,4 beta-glucanase and 'beta '-mannase, and mix that to be prepared as leaven standby;
(5) by standby after adjuvant pulverizing;
(6) adjuvant after pulverizing with animal wastes be in mass ratio 0.01~0.5:1 ratio mix, and add water and be uniformly mixed so as to obtain Water content is the organic fertilizer fermentation culture medium of 40%~80%;
(7) the organic fertilizer fermentation culture medium being prepared into toward step (6) adds leaven prepared by step (4), and mix;
(8) the organic fertilizer fermentation culture medium adding leaven step (7) obtained is packed together, and the THICKNESS CONTROL of material exists 50cm~150cm, and the fermentation materials plastic sheeting building heap is covered good seal, standing for fermentation 7d~40d;
(9) product handling system: fermentation ends, will fermentation after obtain product drying, pulverize and pack after, i.e. prepared fertilizer Finished product.
High temperature resistant facultative anaerobe Methylotrophic bacillus cereus (Bacillus used in described step (1) Methylotrophicus) HL-4 was preserved in Guangdong Province's Culture Collection on January 18th, 2016, and preservation is compiled Number being GDMCC No:60002, preservation address is 5th floors, No. 59 building of compound, Xianlie Middle Road, Guangzhou City 100 Guangdong Province's microbe research Institute.
The inoculum concentration of Bacillus methylotrophicus HL-4 seed liquor used in described step (3) is to access The quality of seed liquor accounts for bran mass gross mass percentages, is 0.1%~5%.
In described step (4), the quality of the addition of the enzyme enzyme to add accounts for the leaven gross mass percentage ratio being prepared as Meter, is respectively as follows: proteinase-10 .1%~15%, cellulase 0.1%~15%, xylanase 0.1%~15%, 1,4 beta-glucanase 0.1% ~15%, 'beta '-mannase 0.1%~15%.
Adjuvant in described step (5) is in straw, bagasse, sawmilling, medicinal residues, mud, manioc waste, tealeaf residue and bark One or more mixture.
Animal wastes in described step (6) are the one in pig manure, cattle manure, sheep stool, birds droppings and chicken manure.
It is total that the quality of the addition of the leaven in described step (7) leaven to add accounts for fertilizer fermentation medium Mass percent, is 0.001%~5%.
Compared with prior art, the technology of the present invention has the following advantages and feature.
(1) whole sweat not turning.The strain of existing organic fertilizer fermentation is all aerobic bacteria, therefore sending out at fertilizer Regular turning is needed during ferment.And the present invention uses high temperature resistant facultative anaerobe Bacillus Methylotrophicus HL-4 has relatively wide fermentation temperature as fermented bacterium, this bacterium, all may be used within 10 DEG C~75 DEG C Fermentation, optimum fermentation temp is 50 DEG C~55 DEG C.This strain is a kind of facultative anaerobe, because ventilating without turning;Additionally should Bacterium is high temperature resistant strain, and its optimum fermentation temp is 50 DEG C~55 DEG C, therefore the heat production of composting fermentation process heats up and is conducive on the contrary The fermentation of strain, thus also it is not required to turning heat radiation.Additionally, the present invention is always according to the concrete condition of fermentation raw material, with the addition of various Fermentable is assisted by enzyme preparation, to reach to complete becoming thoroughly decomposed of fermentation raw material in the case of not turning.With existing skill Art is compared, the present patent application whole sweat not turning.
(2) production cost is significantly reduced.The fertilizer manufacturing technology provided due to the present invention can not turning, thus can Save substantial amounts of equipment investment, it can in addition contain save power consumption and the cost of labor of sweat.Package puts into, moves Power consumes and these aspects artificial, and the present invention can significantly reduce the production cost of fertilizer.
The invention provides the fertilizer manufacture method of a kind of not turning, it not only can save the input of turning device, Labour force and energy consumption cost can also be saved.The fertilizer manufacture method that the present invention provides, is not only applicable to large-scale Organic Fertilizer Plants raw Producing fertilizer, the place that the conditions such as vast rural area and plant that are more suitable for fall behind produces fertilizer simply.
Detailed description of the invention
In order to be more fully understood that the present invention, below with regard to adjuvant, the kind of animal wastes, fermentation parameter and the choosing of fermentation mode Select in conjunction with the embodiments that the invention will be further described, but protection scope of the present invention is not limited to this.
Embodiment 1: the high temperature resistant facultative anaerobe Bacillus methylotrophicus HL-4 of slant preservation is connect Enter after sterilizing cooling LB fluid medium in, in 55 DEG C, 150r/min cultivate 12h, be prepared as Bacillus Methylotrophicus HL-4 seed liquor is standby;It is the ratio mixing of 1:1 in mass ratio by wheat bran and water, and mixes preparation Becoming bran mass, bran mass is standby after 121 DEG C of sterilizing 30min;Bacillus by preparation Methylotrophicus HL-4 seed liquor be linked into sterilizing after cooling bran mass in and mix, then will access bacterium The bran mass planted is divided in the fermentation tray sterilized, and charging thickness is 4cm, and in tray, bran mass surface covers The wet gauze of sterilizing, then tray is placed in the shelf top fermentation in sterilized solid fermentation room, controls ambient temperature at 55 DEG C, Fermentation time is 48h, is prepared as Bacillus after the tunning drying after fermentation ends and pulverizing Methylotrophicus microbial inoculum is standby.By 50 kilograms of Bacillus methylotrophicus microbial inoculums, 10 kilograms of protease, 10 kilograms of cellulase, 10 kilograms of xylanase, 10 kilograms of 1,4 beta-glucanases and 10 kilograms of 'beta '-mannase mixing, and mix It is prepared as leaven standby;By standby after crushed stalk;Take 2 tons of straws pulverized and 8 tons of chicken manure mixing, and add water material Moisture regulates to 60% as organic fertilizer fermentation culture medium;In organic fertilizer fermentation culture medium, addition 10 kilograms is prepared above Leaven, then mix;Carrying out building heap by the organic fertilizer fermentation culture medium adding leaven, the material thickness building heap is 150cm, and the fermentation materials plastic sheeting building heap is covered good seal;Standing for fermentation 20 days;After fermentation ends, fermentation is produced Product can be prepared as fertilizer finished product after being dried and pulverizing.
Embodiment 2: the high temperature resistant facultative anaerobe Bacillus methylotrophicus HL-4 of slant preservation is connect Enter after sterilizing cooling LB fluid medium in, in 55 DEG C, 150r/min cultivate 12h, be prepared as Bacillus Methylotrophicus HL-4 seed liquor is standby;It is the ratio mixing of 1:1 in mass ratio by wheat bran and water, and mixes preparation Becoming bran mass, bran mass is standby after 121 DEG C of sterilizing 30min;Bacillus by preparation Methylotrophicus HL-4 seed liquor be linked into sterilizing after cooling bran mass in and mix, then will access bacterium The bran mass planted is divided in the fermentation tray sterilized, and charging thickness is 4cm, and in tray, fermentation culture primary surface covers The wet gauze of sterilizing, then tray is placed in the shelf top fermentation in sterilized solid fermentation room, controls ambient temperature at 55 DEG C, Fermentation time is 48h, is prepared as Bacillus after the tunning drying after fermentation ends and pulverizing Methylotrophicus microbial inoculum is standby.By 50 kilograms of Bacillus methylotrophicus microbial inoculums, 10 kilograms of protease, 10 kilograms of cellulase, 10 kilograms of xylanase, 10 kilograms of 1,4 beta-glucanases and 10 kilograms of 'beta '-mannase mixing, and mix It is prepared as leaven standby;By standby after bagasse pulverizing;Take 1.5 tons of bagasse pulverized and 8.5 tons of pig manure mixing, and add water The moisture of material is regulated to 65% as organic fertilizer fermentation culture medium;On adding 8 kilograms in organic fertilizer fermentation culture medium Leaven prepared by face, then mixes;Carry out building heap by the organic fertilizer fermentation culture medium adding leaven, build the material thickness of heap For 120cm, and the fermentation materials plastic sheeting building heap is covered good seal;Standing for fermentation 18 days;After fermentation ends, will fermentation Fertilizer finished product can be prepared as after product drying and pulverizing.
Embodiment 3: the high temperature resistant facultative anaerobe Bacillus methylotrophicus HL-4 of slant preservation is connect Enter after sterilizing cooling LB fluid medium in, in 55 DEG C, 150r/min cultivate 12h, be prepared as Bacillus Methylotrophicus HL-4 seed liquor is standby;It is the ratio mixing of 1:1 in mass ratio by wheat bran and water, and mixes preparation Becoming bran mass, bran mass is standby after 121 DEG C of sterilizing 30min;Bacillus by preparation Methylotrophicus HL-4 seed liquor be linked into sterilizing after cooling bran mass in and mix, then will access bacterium The bran mass planted is divided in the fermentation tray sterilized, and charging thickness is 4cm, and in tray, fermentation culture primary surface covers The wet gauze of sterilizing, then tray is placed in the shelf top fermentation in sterilized solid fermentation room, controls ambient temperature at 55 DEG C, Fermentation time is 48h, is prepared as Bacillus after the tunning drying after fermentation ends and pulverizing Methylotrophicus microbial inoculum is standby.By 50 kilograms of Bacillus methylotrophicuss microbial inoculums, 10 kilograms of protease, 10 kilograms of cellulase, 10 kilograms of xylanase, 10 kilograms of 1,4 beta-glucanases and 10 kilograms of 'beta '-mannase mixing, and mix It is prepared as leaven standby;By standby after bark pulverizing;Take 1.5 tons of barks pulverized and 8.5 tons of cattle manure mixing, and add water thing The moisture of material regulates to 65% as organic fertilizer fermentation culture medium;Make above adding 9 kilograms in organic fertilizer fermentation culture medium Standby leaven, then mixes;Carrying out building heap by the organic fertilizer fermentation culture medium adding leaven, the material thickness building heap is 100cm, and the fermentation materials plastic sheeting building heap is covered good seal;Standing for fermentation 15 days;After fermentation ends, fermentation is produced Product can be prepared as fertilizer finished product after being dried and pulverizing.

Claims (7)

1. the fertilizer manufacture method of a not turning, it is characterised in that: this manufacture method comprises the steps:
(1) by the high temperature resistant facultative anaerobe Methylotrophic bacillus cereus (Bacillus methylotrophicus) of slant preservation HL-4 is linked into after sterilizing in the LB fluid medium of cooling, in 55 DEG C, 150r/min cultivate 12h, be prepared as Bacillus Methylotrophicus HL-4 seed liquor is standby;
(2) by wheat bran and water be in mass ratio 1:1 ratio mixing, and mix be prepared as bran mass, bran mass in After 121 DEG C of sterilizing 30min standby;
(3) Bacillus methylotrophicus HL-4 seed liquor step (1) prepared is linked into what step (2) obtained After sterilizing in the bran mass of cooling and mix, then by the bran mass that accesses strain, to be divided in the fermentation sterilized shallow In dish, charging thickness is 4cm, and in tray, fermentation culture primary surface covers the wet gauze of sterilizing, then is placed in by tray sterilized Shelf top fermentation in solid fermentation room, control ambient temperature is at 55 DEG C, and fermentation time is 48h, and the fermentation after fermentation ends is produced It is prepared as Bacillus methylotrophicus microbial inoculum standby after thing drying and pulverizing;
(4) in Bacillus methylotrophicus microbial inoculum prepared by step (3), addition protease, cellulase, wood gather Carbohydrase, 1,4 beta-glucanase and 'beta '-mannase, and mix that to be prepared as leaven standby;
(5) by standby after adjuvant pulverizing;
(6) adjuvant after pulverizing with animal wastes be in mass ratio 0.01~0.5:1 ratio mix, and add water and be uniformly mixed so as to obtain Water content is the organic fertilizer fermentation culture medium of 40%~80%;
(7) the organic fertilizer fermentation culture medium being prepared into toward step (6) adds leaven prepared by step (4), and mix;
(8) the organic fertilizer fermentation culture medium adding leaven step (7) obtained is packed together, and the THICKNESS CONTROL of material exists 50cm~150cm, and the fermentation materials plastic sheeting building heap is covered good seal, standing for fermentation 7d~40d;
(9) product handling system: fermentation ends, will fermentation after obtain product drying, pulverize and pack after, i.e. prepared fertilizer Finished product.
The fertilizer manufacture method of a kind of not turning the most according to claim 1, it is characterised in that: in described step (1) Bacillus methylotrophicus HL-4 used at the deposit number of Guangdong Province's Culture Collection is GDMCC No:60002.
The fertilizer manufacture method of a kind of not turning the most according to claim 1, it is characterised in that: in described step (3) The inoculum concentration of Bacillus methylotrophicus HL-4 seed liquor used accounts for wheat bran training with the quality accessing seed liquor Support base gross mass percentages, be 0.1%~5%.
The fertilizer manufacture method of a kind of not turning the most according to claim 1, it is characterised in that: in described step (4) The addition of enzyme accounts for, with the quality of enzyme added, the leaven gross mass percentages being prepared as, be respectively as follows: proteinase-10 .1%~ 15%, cellulase 0.1%~15%, xylanase 0.1%~15%, 1,4 beta-glucanase 0.1%~15%, 'beta '-mannase 0.1% ~15%.
The fertilizer manufacture method of a kind of not turning the most according to claim 1, it is characterised in that: in described step (5) Adjuvant be one or more mixed in straw, bagasse, sawmilling, medicinal residues, mud, manioc waste, tealeaf residue and bark Compound.
The fertilizer manufacture method of a kind of not turning the most according to claim 1, it is characterised in that: in described step (6) Animal wastes be the one in pig manure, cattle manure, sheep stool, birds droppings and chicken manure.
The fertilizer manufacture method of a kind of not turning the most according to claim 1, it is characterised in that: in described step (7) The addition of leaven account for fertilizer fermentation medium gross mass percentages with the quality of leaven added, be 0.001% ~5%.
CN201610129716.0A 2016-03-09 2016-03-09 Method for producing organic fertilizer without pile turning Pending CN105732143A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106518562A (en) * 2016-12-15 2017-03-22 中国热带农业科学院环境与植物保护研究所 Biological organic fertilizer and preparation method thereof
CN106631579A (en) * 2016-12-08 2017-05-10 南充禾香生物科技有限公司 Small-site organic fertilizer preparation method
CN107021840A (en) * 2017-05-31 2017-08-08 贵州黔源生态农业开发有限公司 A kind of organic fertilizer and preparation method thereof
CN107417357A (en) * 2017-05-31 2017-12-01 贵州黔源生态农业开发有限公司 A kind of organic fertilizer and preparation method thereof
CN110923175A (en) * 2019-12-26 2020-03-27 江苏大道生物环境科技有限公司 Bacillus and application thereof in reduction and resource production of organic fertilizer by kitchen waste

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101225004A (en) * 2008-02-04 2008-07-23 上海创博生态工程有限公司 Method for producing soil biological fertilizer by employing compound starter
CN104761385A (en) * 2015-04-11 2015-07-08 黑龙江八一农垦大学 Multifunctional microbial organic fertilizer and preparation method thereof
TWI508931B (en) * 2014-02-12 2015-11-21

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101225004A (en) * 2008-02-04 2008-07-23 上海创博生态工程有限公司 Method for producing soil biological fertilizer by employing compound starter
TWI508931B (en) * 2014-02-12 2015-11-21
CN104761385A (en) * 2015-04-11 2015-07-08 黑龙江八一农垦大学 Multifunctional microbial organic fertilizer and preparation method thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106631579A (en) * 2016-12-08 2017-05-10 南充禾香生物科技有限公司 Small-site organic fertilizer preparation method
CN106518562A (en) * 2016-12-15 2017-03-22 中国热带农业科学院环境与植物保护研究所 Biological organic fertilizer and preparation method thereof
CN107021840A (en) * 2017-05-31 2017-08-08 贵州黔源生态农业开发有限公司 A kind of organic fertilizer and preparation method thereof
CN107417357A (en) * 2017-05-31 2017-12-01 贵州黔源生态农业开发有限公司 A kind of organic fertilizer and preparation method thereof
CN110923175A (en) * 2019-12-26 2020-03-27 江苏大道生物环境科技有限公司 Bacillus and application thereof in reduction and resource production of organic fertilizer by kitchen waste
CN110923175B (en) * 2019-12-26 2021-05-07 江苏大道生物环境科技有限公司 Bacillus and application thereof in reduction and resource production of organic fertilizer by kitchen waste

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