CN101817707B - Bioorganic pesticide fertilizer and production method thereof - Google Patents

Bioorganic pesticide fertilizer and production method thereof Download PDF

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Publication number
CN101817707B
CN101817707B CN2009102092952A CN200910209295A CN101817707B CN 101817707 B CN101817707 B CN 101817707B CN 2009102092952 A CN2009102092952 A CN 2009102092952A CN 200910209295 A CN200910209295 A CN 200910209295A CN 101817707 B CN101817707 B CN 101817707B
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Prior art keywords
fertilizer
fermentation
bioorganic pesticide
pesticide fertilizer
bioorganic
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CN101817707A (en
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谭周进
曹典军
胡汝晓
尹德明
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Taigu Biotechnology Group Co ltd
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HUNAN TAIGU BIOLOGICAL TECHNOLOGY CO LTD
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses a bioorganic pesticide fertilizer and a production method thereof. The bioorganic pesticide fertilizer is a mixed product prepared from 70 to 80 percent of decomposed crop stalks or pig breeding fermentation bed padding, 5 to 10 percent of medical stone and 15 to 25 percent of inorganic compound fertilizer through effective microorganism fermentation; and the effective microorganism quantity after the fermentation is 2-3*10<8>. The production method comprises the following steps of: mixing the decomposed crop stalks or pig breeding fermentation bed padding and the medical stone; adding 5 to 10 percent of inorganic compound fertilizer first, and then adding effective microorganism bacteria for fermentation at normal temperature; and finally adding the rest 10 to 15 percent of inorganic compound fertilizer into the fermented material. The bioorganic pesticide fertilizer produced by the method can improve the yield of crops, has good effect of preventing and controlling crucifer diseases, and can reduce the nitrate content in brassicaceous vegetables and improve quality; in the production process, agricultural waste is further converted, so the environmental protection is facilitated; and the bioorganic pesticide fertilizer has wide application prospect in agriculture, livestock farming and environmental protection.

Description

A kind of bioorganic pesticide fertilizer and production method thereof
Technical field
The invention belongs to the bioorganic pesticide fertilizer field, particularly the cress disease is had prevention effect, can reduce the biological organic fertilizer of nitrate content in the brassicaceous vegetable.
Background technology
The agricultural product quality problem is the significant problem that is directly connected to people's life, and its quality problems are also influencing our healthy.A large amount of continuous croppings of plant, continuous cropping for providing of sick worm favourable ecotope, cause the kind of crop pest increasing.In controlling crop diseases and insect pests, hypertoxic, the high poison of use, persistent pesticide are prohibited and incessantly, are caused the pesticide residue in eubiosis destruction, the soil, and the use of chemical fertilizer has become the soil physical property change, nitrate accumulates in a large number, has brought serious harm to people.
The application of bio-feritlizer all has greatly improved to the yield and qualities of vegetables.Photosynthetic bacterium is used as bio-feritlizer can improve tomato, and the output of cucumber is improved the quality of crop; Improve the content of crop chlorophyll content and Vc; Reduce the content of nitrite in the vegetables, prolong the preservation period of crop.Investigation in the city, Fushun City shows that microbial fertilizer can reduce capsicum, Chinese cabbage and eggplant nitrate content, improves Vc content in capsicum and the Chinese cabbage.Using refining environmental protection fertilizer on Plantula Brassicae chinensis can significantly promote it to grow, plant height increases by 41.8%, the number of blade increases by 18.2%, output improves 44.3%, reach extremely significantly volume increase level, full nitrogen, aminoacids content improve 0.044,0.032 percentage point respectively in the blade, and nitrate and nitrite content descend respectively 203.2mg/kg and 0.4mg/kg.Use the output that bio-bacterial manure can improve Plantula Brassicae chinensis, amount of increase in production reaches 30%-40%, reduces the content of nitrate in the Plantula Brassicae chinensis, than single reduction 500-800m/kg to fertilize.Safe composite biological fertilizer of a specified duration can obviously reduce the Plantula Brassicae chinensis nitrate content, generally can reduce 20%-30%, improves quality.Do on the basis of base manure using an amount of composite fertilizer, increase 30kg.hm -2Bacteria agent, can obviously improve soil for the nitrogen ability, but nitrate content is the highest in the plant body, reaches 3219.5mg.kg -1, (national standard of leaf vegetables nitrate content is≤3000mg.kg the content that is above standard -1).
Summary of the invention
The purpose of this invention is to provide and a kind of the cress disease is had prevention effect, the biological organic fertilizer that can reduce nitrate content in the brassicaceous vegetable and production method thereof.
The objective of the invention is to realize by following scheme.
The bioorganic pesticide fertilizer solid material that the agricultural crop straw that a kind of bioorganic pesticide fertilizer is become thoroughly decomposed by 70-80wt% or the fermenting bed padding of raising pigs, 5-10wt% medical stone, 15-25wt% inorganic composite mixed fertilizer are formed, inoculation accounts for the mix products of the effective microbe fermentation back gained of bioorganic pesticide fertilizer solid material weight 5-10%, and wherein effective microbe quantity reaches 2-3 * 10 8CFU/g.
The described agricultural crop straw that becomes thoroughly decomposed is that agricultural crop straw is crushed to 1-30 millimeter coarse particles size, adds the mould solid spawn of ACCC30557 aspergillus niger and ACCC30371 Ha Shi wood; Wherein the mould spore count of aspergillus niger and Ha Shi wood is 1: 1, and inoculum size is 5-10wt%, obtains in heap fermentation 25-30 days.
The described fermenting bed padding of raising pigs is with the 40-55wt% sawdust, the 45-50wt% husk, and the 1-2wt% microbial starter culture, fermentation obtained in 1 year-2 years in the circle of raising pigs; Described microbial starter culture is made up of by 1: 1 thalline quantity ACCC20157 yeast saccharomyces cerevisiae and ACCC10148 Bacillus subtilus.
The nutrient N+P of described inorganic composite mixed fertilizer 2O 5+ K 2O 〉=45.0%.
Described effective microbe is mixed by 1: 1 thalline quantity by ACCC10613 Bacillus licheniformis and ACCC10148 Bacillus subtilus and forms.
The production method of described bioorganic pesticide fertilizer: the agricultural crop straw or the fermenting bed padding of raising pigs that 70-80wt% is become thoroughly decomposed, the inorganic composite mixed fertilizer of 5-10wt% medical stone and 15-25wt% is formed the bioorganic pesticide fertilizer solid material, inoculate the effective microbe of the 5-10% that accounts for bioorganic pesticide fertilizer solid material gross weight, make effective microbe quantity wherein reach 0.2-0.5 * 10 8CFU/g, material thickness remains on 30-50 centimetre then, is piled up in the normal temperature bottom fermentation 3-4 days, stirs 1-2 time in the fermenting process, makes effective microbe quantity reach 2-3 * 10 8CFU/g obtains described bioorganic pesticide fertilizer, and wherein inorganic composite mixed fertilizer adds at twice, adds 5-10wt% earlier, adds remaining 10-15wt% again after the fermentation of inoculation effective microbe.
Bacillus subtilus among the present invention and Bacillus licheniformis have the effect of antagonism Bacteria erwinia and alternaria, and have nitrate reductase activity, contain trace element and rare earth elements such as K, Na, Ca, Mg, Cu, Mo in the medical stone.Bacillus subtilus that medical stone and the present invention are used and Bacillus licheniformis are organic when being combined together, the Bacteria erwinia and the alternaria that can effectively suppress brassicaceous vegetable, simultaneously, microorganism among the present invention, medical stone and organic use can further effectively reduce the nitrate content in the vegetables.This invention raw material is equipped with inorganic composite mixed fertilizer from agricultural wastes, by with the mix and match of medical stone, through action of microorganisms, collection fertilizer efficiency, diseases prevention and reduction nitrate content are one.The bioorganic pesticide fertilizer that the present invention produces can be used in control brassicaceous vegetable disease (is main object with soft rot), reduces the nitrate content of vegetables, improves the yield and quality of vegetables, and the production of pollution-free vegetable is had important effect.
Embodiment
Following examples are intended to further specify the present invention, rather than restriction the present invention.
Embodiment 1:
Effective microbe bacterial classification in the fertilizer making processes is ACCC10613 Bacillus licheniformis and ACCC10148 Bacillus subtilus, all buys from Chinese agriculture microbial strains preservation center.
Inorganic composite mixed fertilizer: for Hunan Xiangzhu Chemical Industry Co., Ltd produces, nutrient N+P 2O 5+ K 2O 〉=45.0%.
The Bacillus licheniformis substratum: 10% malt extract medium, not adding agar is liquid nutrient medium, adding 2% agar is solid medium.
The Bacillus subtilus substratum: nutrient broth, not adding agar is liquid nutrient medium, adding 2% agar is solid medium.
Above-mentioned bacterial classification is seeded in respectively on the corresponding inclined-plane activates, be inoculated into then and cultivate the preparation liquid spawn in the corresponding triangular flask liquid nutrient medium, culture temperature is 30 ℃, and the vibration revolution of liquid spawn is 150 rev/mins, and incubation time is 40 hours.
The cultivation of Bacillus licheniformis and Bacillus subtilus: get the aforesaid liquid substratum and inoculate corresponding liquid spawn respectively, 30 ℃, 150 rev/mins shaking culture 40 hours by 5% inoculum size.
With 80wt% raise pigs fermenting bed padding (with the 40-55wt% sawdust, the 45-50wt% husk, the 1-2wt% microbial starter culture, fermentation obtained in 1 year-2 years in the circle of raising pigs; Described microbial starter culture is made up of by 1: 1 thalline quantity ACCC20157 yeast saccharomyces cerevisiae and ACCC10148 Bacillus subtilus, bacterial classification is bought from Chinese agriculture microbial strains preservation center), (inorganic composite mixed fertilizer adds the inorganic composite mixed fertilizer of 5wt% medical stone and 15wt% at twice in the actually operating, here add 5wt% earlier, etc. adding remaining 10wt% again after the fermentation of follow-up inoculation effective microbe) form the bioorganic pesticide fertilizer solid material, mix, inoculate the cultivation mixed solution (mix form by 1: 1 thalline quantity) of 9% the above-mentioned Bacillus licheniformis that accounts for bioorganic pesticide fertilizer solid material gross weight and Bacillus subtilus, make effective microbe quantity wherein reach 0.25 * 10 8CFU/g (separating counting process by selective medium with dilution plate detects), material thickness remains on 35 centimetres then, is piled up in the normal temperature bottom fermentation 4 days, stirs in the fermenting process 2 times, promptly gets head product of the present invention.
Production: add remaining 10wt% inorganic composite mixed fertilizer in above-mentioned head product, mix, the effective microbe quantity of this moment reaches 2-3 * 10 8CFU/g, other every indexs reach NY884-2004.
The field test design:
Trial crops is a Plantula Brassicae chinensis, and every plot experiment area is 67m 2, test is established and is handled and contrast, handles the bioorganic pesticide fertilizer of using the present invention's preparation, the inorganic composite mixed fertilizer that Hunan Xiangzhu Chemical Industry Co., Ltd produces is used in contrast, the nutrient insufficient section that bioorganic pesticide fertilizer is handled replenishes with chemical fertilizer, all makes base manure and once applies Routine Management.Respectively establish 8 repetitions.
Field test effect:
The experiment treatment zone is compared with the experiment contrast district, treatment zone Chinese cabbage volume increase 38.9%, and nitrate content decline 309.5mg/kg, the soft rot incidence reduces by 95%, and the black spot incidence reduces by 99%, all reaches utmost point conspicuous level.
Embodiment 2:
Becoming thoroughly decomposed of agricultural crop straw: agricultural crop straw is pulverized (degree of grinding is 18 millimeters thicknesses), (wherein the mould spore count of aspergillus niger and Ha Shi wood is 1: 1 to add the mould solid spawn of ACCC30557 aspergillus niger and ACCC30371 Ha Shi wood, inoculum size is 8%, bacterial classification is bought from Chinese agriculture microbial strains preservation center), promptly can be used as raw material of the present invention in heap fermentation 25-30 days.
Effective microbe bacterial classification in the fertilizer making processes is ACCC10613 Bacillus licheniformis and ACCC10148 Bacillus subtilus, all buys from Chinese agriculture microbial strains center.
Inorganic composite mixed fertilizer: for Hunan Xiangzhu Chemical Industry Co., Ltd produces, nutrient N+P 2O 5+ K 2O 〉=45.0%.
The Bacillus licheniformis substratum: 10% malt extract medium, not adding agar is liquid nutrient medium, adding 2% agar is solid medium.
The Bacillus subtilus substratum: nutrient broth, not adding agar is liquid nutrient medium, adding 2% agar is solid medium.
Above-mentioned bacterial classification is seeded in respectively on the corresponding inclined-plane activates, be inoculated into then and cultivate the preparation liquid spawn in the corresponding triangular flask liquid nutrient medium, culture temperature is 30 ℃, and the vibration revolution of liquid spawn is 150 rev/mins, and incubation time is 40 hours.
The cultivation of Bacillus licheniformis and Bacillus subtilus: get the aforesaid liquid substratum and inoculate corresponding liquid spawn respectively, 30 ℃, 150 rev/mins shaking culture 40 hours by 5% inoculum size.
The agricultural crop straw that above-mentioned 70wt% is become thoroughly decomposed, (inorganic composite mixed fertilizer adds the inorganic composite mixed fertilizer of 10wt% medical stone and 20wt% at twice in the actually operating, here add 10wt% earlier, etc. adding remaining 10wt% again after the fermentation of follow-up inoculation effective microbe) form the bioorganic pesticide fertilizer solid material, mix, inoculate the cultivation mixed solution (mix form by 1: 1 thalline quantity) of 9% the above-mentioned Bacillus licheniformis that accounts for bioorganic pesticide fertilizer solid material gross weight and Bacillus subtilus, make effective microbe quantity wherein reach 0.25 * 10 8CFU/g (separating counting process by selective medium with dilution plate detects), material thickness remains on 35 centimetres then, is piled up in the normal temperature bottom fermentation 4 days, stirs in the fermenting process 2 times, promptly gets head product of the present invention.
Production: add remaining 10wt% inorganic composite mixed fertilizer in above-mentioned head product, mix, the effective microbe quantity of this moment reaches 2-3 * 10 8CFU/g, other every indexs reach NY884-2004.
Field test design, field test effect are all undertaken by embodiment 1.
The experiment treatment zone is compared with the experiment contrast district, treatment zone Chinese cabbage volume increase 40.1%, and nitrate content decline 328.9mg/kg, the soft rot incidence reduces by 97%, and the black spot incidence reduces by 98%, all reaches utmost point conspicuous level.

Claims (2)

1. bioorganic pesticide fertilizer, it is characterized in that, the bioorganic pesticide fertilizer solid material that the agricultural crop straw that is become thoroughly decomposed by 70-80wt% or the fermenting bed padding of raising pigs, 5-10wt% medical stone, 15-25wt% inorganic composite mixed fertilizer are formed, inoculation accounts for the mix products of the effective microbe fermentation back gained of bioorganic pesticide fertilizer solid material weight 5-10%, and wherein effective microbe quantity reaches 2-3 * 10 8CFU/g; The described agricultural crop straw that becomes thoroughly decomposed is that agricultural crop straw is crushed to 1-30 millimeter coarse particles size, adds ACCC30557 aspergillus niger (Aspergillus niger) and ACCC30371 Ha Shi wood mould (Trichoderma harzianum) solid spawn; Wherein the mould spore count of aspergillus niger and Ha Shi wood is 1: 1, and inoculum size is 5-10wt%, obtains in heap fermentation 25-30 days; The described fermenting bed padding of raising pigs is with the 40-55wt% sawdust, the 45-50wt% husk, and the 1-2wt% microbial starter culture, fermentation obtained in 1 year-2 years in the circle of raising pigs; Described microbial starter culture is made up of by 1: 1 thalline quantity ACCC20157 yeast saccharomyces cerevisiae (Saccharomyces cerevisiae) and ACCC10148 Bacillus subtilus (Bacillus subtilis); Described effective microbe is mixed by 1: 1 thalline quantity by ACCC10613 Bacillus licheniformis (Bacillus licheniformis) and ACCC10148 Bacillus subtilus and forms; The nutrient N+P of described inorganic composite mixed fertilizer 2O 5+ K 2O 〉=45.0%.
2. the production method of the described bioorganic pesticide fertilizer of claim 1, it is characterized in that, the agricultural crop straw or the fermenting bed padding of raising pigs that 70-80wt% is become thoroughly decomposed, the inorganic composite mixed fertilizer of 5-10wt% medical stone and 15-25wt% is formed the bioorganic pesticide fertilizer solid material, inoculate the effective microbe of the 5-10% that accounts for bioorganic pesticide fertilizer solid material gross weight, make effective microbe quantity wherein reach 0.2-0.5 * 10 8CFU/g, material thickness remains on 30-50 centimetre then, is piled up in the normal temperature bottom fermentation 3-4 days, stirs 1-2 time in the fermenting process, makes effective microbe quantity reach 2-3 * 10 8CFU/g obtains described bioorganic pesticide fertilizer, and wherein inorganic composite mixed fertilizer adds at twice, adds 5-10wt% earlier, adds remaining 10-15wt% again after the fermentation of inoculation effective microbe.
CN2009102092952A 2009-10-29 2009-10-29 Bioorganic pesticide fertilizer and production method thereof Expired - Fee Related CN101817707B (en)

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CN101948754A (en) * 2010-09-06 2011-01-19 成都和谐生物科技有限公司 Microbial fermentation bed strain and manufacturing method thereof
CN103570392A (en) * 2013-10-29 2014-02-12 太仓市双凤勤之立生态养殖场 Composting treatment method for waste pig raising fermentation bed
CN103880491B (en) * 2014-04-09 2016-02-03 刘骅毅 Bio-organic fertilizer of a kind of prevention and control soil continuous cropping obstacle and preparation method thereof
CN104311171A (en) * 2014-10-08 2015-01-28 南京农业大学 Method using packing of fermentation bed as biological organic fertilizer
CN104311288A (en) * 2014-10-10 2015-01-28 安徽万利生态园林景观有限公司 Eggplant nursery base fertilizer made from waste mattress material and preparation method of eggplant nursery base fertilizer
CN104311287A (en) * 2014-10-10 2015-01-28 安徽万利生态园林景观有限公司 Special base fertilizer for watermelon nursery and preparation method thereof
CN104230578A (en) * 2014-10-10 2014-12-24 安徽万利生态园林景观有限公司 Base fertilizer used for bean nursery garden and preparation method thereof
CN104311286A (en) * 2014-10-10 2015-01-28 安徽万利生态园林景观有限公司 Base fertilizer used for cabbage nursery and made of fermentation bedding for pigs and preparation method of base fertilizer
CN104788239B (en) * 2015-04-29 2017-12-22 新化县维山糯米种植专业合作社 A kind of glutinous rice rice special bio organic medical fertilizer
CN105367222A (en) * 2015-11-10 2016-03-02 桃江县新星生物科技有限公司 Biological organic fertilizer and preparation method thereof
CN106187340A (en) * 2016-07-01 2016-12-07 福建富生源农业科技发展有限公司 A kind of cultivation Bag Material improving arginine content in Morchella esculenta (L.) Pers sporophore
CN106010576A (en) * 2016-07-13 2016-10-12 湖南泰谷生物科技股份有限公司 Composite soil conditioner and application thereof
CN106542933A (en) * 2016-11-25 2017-03-29 济南七彩牧歌生态农业有限公司 The method that a kind of discarded bedding and padding of fermentation bed prepare fertilizer
CN106929447B (en) * 2017-03-21 2020-07-24 南京农业大学 Bacillus licheniformis B L06 and application thereof
CN107721593A (en) * 2017-11-02 2018-02-23 广西壮族自治区金秀瑶族自治县科学技术情报研究所 Chinese yew slow release fertilizer and preparation method thereof

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CN1858028A (en) * 2005-04-30 2006-11-08 张绍勋 Method for producing turf humic acid compound fertilizer hy composite fermenting and degradation method
CN101250072A (en) * 2007-10-10 2008-08-27 焦俊武 Multi-element organic mineral fertilizer
CN101475410A (en) * 2009-01-20 2009-07-08 湖南泰谷生物科技有限责任公司 Organic fertilizer and method for producing the same

Patent Citations (4)

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Publication number Priority date Publication date Assignee Title
CN1308038A (en) * 2001-02-16 2001-08-15 何策熙 Biological organic composite fertilizer and its production process
CN1858028A (en) * 2005-04-30 2006-11-08 张绍勋 Method for producing turf humic acid compound fertilizer hy composite fermenting and degradation method
CN101250072A (en) * 2007-10-10 2008-08-27 焦俊武 Multi-element organic mineral fertilizer
CN101475410A (en) * 2009-01-20 2009-07-08 湖南泰谷生物科技有限责任公司 Organic fertilizer and method for producing the same

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