CN112939637A - Preparation method of probiotics fermented carbon-energy organic fertilizer based on cotton waste residue mixing - Google Patents
Preparation method of probiotics fermented carbon-energy organic fertilizer based on cotton waste residue mixing Download PDFInfo
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- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 134
- 229920000742 Cotton Polymers 0.000 title claims abstract description 118
- 239000002699 waste material Substances 0.000 title claims abstract description 115
- 239000006041 probiotic Substances 0.000 title claims abstract description 65
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 65
- 238000002156 mixing Methods 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 30
- 238000006243 chemical reaction Methods 0.000 claims abstract description 76
- 239000000203 mixture Substances 0.000 claims abstract description 76
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 59
- 238000000855 fermentation Methods 0.000 claims abstract description 54
- 230000004151 fermentation Effects 0.000 claims abstract description 46
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 39
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 39
- 230000000529 probiotic effect Effects 0.000 claims abstract description 34
- 238000000034 method Methods 0.000 claims abstract description 18
- 239000002002 slurry Substances 0.000 claims abstract description 17
- 241000283690 Bos taurus Species 0.000 claims abstract description 9
- 241001494479 Pecora Species 0.000 claims abstract description 9
- 238000011049 filling Methods 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims abstract description 9
- 239000003337 fertilizer Substances 0.000 claims description 42
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 32
- 239000003153 chemical reaction reagent Substances 0.000 claims description 32
- 239000012153 distilled water Substances 0.000 claims description 32
- 239000007788 liquid Substances 0.000 claims description 32
- 238000003756 stirring Methods 0.000 claims description 30
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 20
- 230000001079 digestive effect Effects 0.000 claims description 16
- 239000000706 filtrate Substances 0.000 claims description 16
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 16
- 239000011521 glass Substances 0.000 claims description 16
- 238000010438 heat treatment Methods 0.000 claims description 16
- 241000894006 Bacteria Species 0.000 claims description 10
- 229910052757 nitrogen Inorganic materials 0.000 claims description 10
- 239000005416 organic matter Substances 0.000 claims description 9
- 238000001816 cooling Methods 0.000 claims description 8
- 238000001514 detection method Methods 0.000 claims description 8
- 230000029087 digestion Effects 0.000 claims description 8
- 239000004744 fabric Substances 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 8
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 8
- 238000005303 weighing Methods 0.000 claims description 8
- 239000000126 substance Substances 0.000 abstract description 8
- 241000219146 Gossypium Species 0.000 description 92
- 239000000835 fiber Substances 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 235000012343 cottonseed oil Nutrition 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- MCSXGCZMEPXKIW-UHFFFAOYSA-N 3-hydroxy-4-[(4-methyl-2-nitrophenyl)diazenyl]-N-(3-nitrophenyl)naphthalene-2-carboxamide Chemical compound Cc1ccc(N=Nc2c(O)c(cc3ccccc23)C(=O)Nc2cccc(c2)[N+]([O-])=O)c(c1)[N+]([O-])=O MCSXGCZMEPXKIW-UHFFFAOYSA-N 0.000 description 1
- 240000002024 Gossypium herbaceum Species 0.000 description 1
- 235000004341 Gossypium herbaceum Nutrition 0.000 description 1
- 241000219071 Malvaceae Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000010828 animal waste Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000003575 carbonaceous material Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000001863 plant nutrition Effects 0.000 description 1
- 239000010908 plant waste Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Fertilizers (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a preparation method of a probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing, which comprises the following steps: the method comprises the following steps: opening a large reaction tank in the open air, and then pulling, transporting and filling the prepared cotton waste residues by a transport vehicle; step two: directly watering the inside of an open reaction tank, then using a stirrer to fully mix water and cotton waste residues, after one month, pushing a carbon energy organic fertilizer formed after natural fermentation to an open flat ground by using a forklift, or preparing a mixture of biogas slurry and water with a certain dosage, or cattle and sheep excrement, and naming the materials as a component A; step three: preparing a certain dosage of probiotics and zymocyte, and naming the probiotics and zymocyte as a component B; according to the invention, the open-air reaction tank is adopted, so that the mode of fermenting the cotton waste residues into the carbon energy organic fertilizer is more convenient and faster, excessive chemical instruments are not required, and the fermentation process of the cotton waste residues is greatly facilitated.
Description
Technical Field
The invention belongs to the technical field of organic fertilizer preparation, and particularly relates to a preparation method of a probiotic fermented carbon energy organic fertilizer based on cotton waste residue mixing.
Background
Cotton, a seed fiber of cotton plants of the Malvaceae family, is native to subtropics. The plant is shrubbery and can grow to 6 meters in height, generally 1 to 2 meters, when cultivated in tropical regions. Flowers are milky white and turn deep red shortly after flowering and then wither, leaving a small green capsule called boll. The cotton bolls are filled with cotton seeds, fuzz on the cotton seeds grows out of the cotton seed skins and fills the cotton bolls, and the cotton bolls crack when mature to expose soft fibers. The fiber is white or white with yellow, the length of the fiber is about 2-4 cm (0.75-1.5 inches), the fiber contains about 87-90% of cellulose, 5-8% of water and 4-6% of other substances. The countries with the highest cotton yield are China, America, India and the like.
Probiotics are active microorganisms which are beneficial to a host and change the composition of flora at a certain part of the host by colonizing in a human body. The health of the intestinal tract is kept by promoting the absorption of nutrients by regulating the immune function of the host mucous membrane and the system or by regulating the balance of flora in the intestinal tract, so that single microorganisms or mixed microorganisms with definite compositions which are beneficial to the health are generated.
Carbonaceous materials, primarily from plants and animals, which are applied to the soil to provide plant nutrition as their primary function. Is prepared from biological substances, animal and plant wastes and plant residues, eliminates toxic and harmful substances in the biological substances, and is rich in a large amount of beneficial substances, including: various organic acids, peptides and rich nutrient elements including nitrogen, phosphorus and potassium. The fertilizer not only can provide comprehensive nutrition for crops, but also has long fertilizer efficiency, can increase and update soil organic matters, promote microbial propagation, improve the physical and chemical properties and biological activity of soil, and is a main nutrient for green food production.
At present, the common treatment mode of the traditional cotton waste residue is incineration treatment, the mode not only causes serious pollution to the atmosphere, but also can not recycle the cotton waste residue, and the waste is very waste.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, and provides a preparation method of a probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing, so as to solve the problems of serious air pollution, incapability of recycling the cotton waste residue and waste in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a preparation method of a probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing comprises the following steps:
the method comprises the following steps: opening a large reaction tank in the open air, and then pulling, transporting and filling the prepared cotton waste residues by a transport vehicle;
step two: directly watering the inside of an open reaction tank, then using a stirrer to fully mix water and cotton waste residues, after one month, pushing a carbon energy organic fertilizer formed after natural fermentation to an open flat ground by using a forklift, or preparing a mixture of biogas slurry and water with a certain dosage, or cattle and sheep excrement, and naming the materials as a component A;
step three: preparing a certain dosage of probiotics and zymocyte, and naming the probiotics and zymocyte as a component B;
step four: mixing the component A and the component B according to the proportion of 1: 0.1-0.5, and then evenly stirring and mixing to form a component C;
step five: uniformly pouring the component C on the surface of cotton waste residue in an open-air reaction tank;
step six: stirring the cotton waste residue and the component C in the open-air reaction tank by using a stirrer, and repeatedly stirring for 2-3 times to ensure that the cotton waste residue and the component C in the open-air reaction tank naturally react to form a carbon-energy organic fertilizer;
step seven: after one month, the carbon energy organic fertilizer after the reaction is transported on an open flat ground by a forklift, the carbon energy organic fertilizer is piled up to a height of about 1.5 m, a layer of plastic cloth is wrapped on the surface of the organic fertilizer, and the organic fertilizer is formed through continuous fermentation;
step eight: weighing 0.5g of organic fertilizer sample, putting the organic fertilizer sample into a glass triangular flask, sequentially adding 5.0mL of concentrated sulfuric acid and 5-10 drops of organic fertilizer digestion accelerator, shaking up gently, putting a small bent-neck funnel on a bottle mouth, heating the mixture on an electric furnace at a low temperature for 10 minutes, observing, heating the mixture until the mixture is digested to white, taking down the mixture, and cooling the mixture to obtain a digestive juice;
step nine: carefully transferring the treated digestive juice into a 100mL volumetric flask by using distilled water, adding the distilled water to a scale mark for constant volume, shaking up, filtering into the triangular flask, discarding the initial filtrate if the triangular flask is not dry, sucking 2mL of the filtrate into the 100mL volumetric flask, keeping the constant volume by using the distilled water, and shaking up to obtain a solution to be detected;
step ten: and (3) respectively sucking distilled water by using a 5mL suction pipe to serve as blank liquid, and respectively adding 2.0mL of the fertilizer standard liquid and the liquid to be detected into three small glass bottles in sequence: 4 drops of fertilizer ammonium nitrogen No. 1 reagent, 4 drops of fertilizer ammonium nitrogen No. 2 reagent, 4 drops of fertilizer ammonium nitrogen No. 3 reagent and 2 drops of fertilizer ammonium nitrogen No. 4 reagent are shaken up, after standing for 10 minutes, the mixture is respectively transferred into three cuvettes and is measured on a computer, the value displayed at the moment is the nitrogen content of the organic fertilizer, the detection of the organic matter content in the organic fertilizer is continued according to the method, and then the data is recorded.
Preferably, in the first step, the open air reaction tank has a length of 5 meters, a width of 3 meters and a height of 1 meter.
Preferably, in the step two, in the component A, the ratio of the biogas slurry to the water mixture is controlled to be 1: about 1.
Preferably, in the third step, the preparation method of the probiotics and the fermentation mother bacteria comprises the following steps: taking out a certain dosage of probiotic strains and fermentation mother strains, and performing liquid anaerobic fermentation to form the probiotic and the fermentation mother strains.
Preferably, in the fifth step, the component C is uniformly poured on the surface of the cotton waste residues and exceeds the surface of the cotton waste residues by 5-10 cm.
Preferably, in the sixth step, when the cotton waste residue and the C component in the open-air reaction tank are stirred, the C component and the cotton waste residue are fully and uniformly stirred by adopting a telescopic stirrer, so that the cotton waste residue and the C component are prevented from being insufficiently fermented, and the finally prepared organic fertilizer is not qualified.
Preferably, in the seventh step, when the carbon-energy organic fertilizer is fermented to be completely black and is grabbed by hands, the carbon-energy organic fertilizer is in a mycelium shape.
Compared with the prior art, the invention provides a preparation method of a probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing, which has the following beneficial effects:
1. according to the invention, the open-air reaction tank is adopted, so that the mode of fermenting the cotton waste residues into the carbon energy organic fertilizer is more convenient and faster, excessive chemical instruments are not needed, the fermentation process of the cotton waste residues is greatly facilitated, the size of the open-air reaction tank is set to be 5 multiplied by 3 multiplied by 1 meter, the size of the reaction tank is more suitable for observation, and the problem of incomplete internal reaction caused by overlarge traditional reaction tank is avoided;
2. according to the invention, the component C is uniformly poured on the surface of the cotton waste residue and exceeds the surface of the cotton waste residue by 5-10 cm, so that probiotics and fermentation mother bacteria in the component C are effectively and fully reacted with the cotton waste residue in the open-air reaction tank, incomplete reaction caused by too few probiotics and fermentation mother bacteria is prevented, and finally unqualified preparation of the carbon energy organic fertilizer is prevented, so that the organic fertilizer cannot be used.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a technical scheme that: a preparation method of a probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing comprises the following steps:
the method comprises the following steps: opening a large reaction tank in the open air, and then pulling, transporting and filling the prepared cotton waste residues by a transport vehicle;
step two: directly watering the inside of an open reaction tank, then using a stirrer to fully mix water and cotton waste residues, after one month, pushing a carbon energy organic fertilizer formed after natural fermentation to an open flat ground by using a forklift, or preparing a mixture of biogas slurry and water with a certain dosage, or cattle and sheep excrement, and naming the materials as a component A;
step three: preparing a certain dosage of probiotics and zymocyte, and naming the probiotics and zymocyte as a component B;
step four: mixing the component A and the component B according to the proportion of 1: 0.1-0.5, and then evenly stirring and mixing to form a component C;
step five: uniformly pouring the component C on the surface of cotton waste residue in an open-air reaction tank;
step six: stirring the cotton waste residue and the component C in the open-air reaction tank by using a stirrer, and repeatedly stirring for 2-3 times to ensure that the cotton waste residue and the component C in the open-air reaction tank naturally react to form a carbon-energy organic fertilizer;
step seven: after one month, the carbon energy organic fertilizer after the reaction is transported on an open flat ground by a forklift, the carbon energy organic fertilizer is piled up to a height of about 1.5 m, a layer of plastic cloth is wrapped on the surface of the organic fertilizer, and the organic fertilizer is formed through continuous fermentation;
step eight: weighing 0.5g of organic fertilizer sample, putting the organic fertilizer sample into a glass triangular flask, sequentially adding 5.0mL of concentrated sulfuric acid and 5-10 drops of organic fertilizer digestion accelerator, shaking up gently, putting a small bent-neck funnel on a bottle mouth, heating the mixture on an electric furnace at a low temperature for 10 minutes, observing, heating the mixture until the mixture is digested to white, taking down the mixture, and cooling the mixture to obtain a digestive juice;
step nine: carefully transferring the treated digestive juice into a 100mL volumetric flask by using distilled water, adding the distilled water to a scale mark for constant volume, shaking up, filtering into the triangular flask, discarding the initial filtrate if the triangular flask is not dry, sucking 2mL of the filtrate into the 100mL volumetric flask, keeping the constant volume by using the distilled water, and shaking up to obtain a solution to be detected;
step ten: and (3) respectively sucking distilled water by using a 5mL suction pipe to serve as blank liquid, and respectively adding 2.0mL of the fertilizer standard liquid and the liquid to be detected into three small glass bottles in sequence: 4 drops of fertilizer ammonium nitrogen No. 1 reagent, 4 drops of fertilizer ammonium nitrogen No. 2 reagent, 4 drops of fertilizer ammonium nitrogen No. 3 reagent and 2 drops of fertilizer ammonium nitrogen No. 4 reagent are shaken up, after standing for 10 minutes, the mixture is respectively transferred into three cuvettes and is measured on a computer, the value displayed at the moment is the nitrogen content of the organic fertilizer, the detection of the organic matter content in the organic fertilizer is continued according to the method, and then the data is recorded.
In the first step, the length of the open-air reaction tank is 5 meters, the width is 3 meters, and the height is 1 meter.
In the step two, in the component A, the ratio of the biogas slurry to the water mixture is controlled to be 1: about 1.
In the third step, the preparation method of the probiotics and the fermentation mother bacteria comprises the following steps: taking out a certain dosage of probiotic strains and fermentation mother strains, and performing liquid anaerobic fermentation to form the probiotic and the fermentation mother strains.
In the fifth step, the component C is uniformly poured on the surface of the cotton waste residue and exceeds the surface of the cotton waste residue by 5-10 cm.
And step six, when the cotton waste residue and the C component in the open-air reaction tank are stirred, a telescopic stirrer is adopted to fully and uniformly stir the C component and the cotton waste residue, so that the cotton waste residue and the C component are prevented from being insufficiently fermented, and the finally prepared organic fertilizer is not qualified.
And seventhly, fermenting the carbon energy organic fertilizer into complete black, and grabbing the black by hands until the black is in a mycelium shape.
Example one
A preparation method of a probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing comprises the following steps:
the method comprises the following steps: opening a large reaction tank in the open air, and then pulling, transporting and filling the prepared cotton waste residues by a transport vehicle;
step two: directly watering the inside of an open reaction tank, then using a stirrer to fully mix water and cotton waste residues, after one month, pushing a carbon energy organic fertilizer formed after natural fermentation to an open flat ground by using a forklift, or preparing a mixture of biogas slurry and water with a certain dosage, or cattle and sheep excrement, and naming the materials as a component A;
step three: preparing a certain dosage of probiotics and zymocyte, and naming the probiotics and zymocyte as a component B;
step four: mixing the component A and the component B according to the proportion of 1: 0.1, stirring and mixing uniformly to form a component C;
step five: uniformly pouring the component C on the surface of cotton waste residue in an open-air reaction tank;
step six: stirring the cotton waste residue and the component C in the open-air reaction tank by using a stirrer, and repeatedly stirring for 2-3 times to ensure that the cotton waste residue and the component C in the open-air reaction tank naturally react to form a carbon-energy organic fertilizer;
step seven: after one month, the carbon energy organic fertilizer after the reaction is transported on an open flat ground by a forklift, the carbon energy organic fertilizer is piled up to a height of about 1.5 m, a layer of plastic cloth is wrapped on the surface of the organic fertilizer, and the organic fertilizer is formed through continuous fermentation;
step eight: weighing 0.5g of organic fertilizer sample, putting the organic fertilizer sample into a glass triangular flask, sequentially adding 5.0mL of concentrated sulfuric acid and 5-10 drops of organic fertilizer digestion accelerator, shaking up gently, putting a small bent-neck funnel on a bottle mouth, heating the mixture on an electric furnace at a low temperature for 10 minutes, observing, heating the mixture until the mixture is digested to white, taking down the mixture, and cooling the mixture to obtain a digestive juice;
step nine: carefully transferring the treated digestive juice into a 100mL volumetric flask by using distilled water, adding the distilled water to a scale mark for constant volume, shaking up, filtering into the triangular flask, discarding the initial filtrate if the triangular flask is not dry, sucking 2mL of the filtrate into the 100mL volumetric flask, keeping the constant volume by using the distilled water, and shaking up to obtain a solution to be detected;
step ten: and (3) respectively sucking distilled water by using a 5mL suction pipe to serve as blank liquid, and respectively adding 2.0mL of the fertilizer standard liquid and the liquid to be detected into three small glass bottles in sequence: 4 drops of fertilizer ammonium nitrogen No. 1 reagent, 4 drops of fertilizer ammonium nitrogen No. 2 reagent, 4 drops of fertilizer ammonium nitrogen No. 3 reagent and 2 drops of fertilizer ammonium nitrogen No. 4 reagent are shaken up, after standing for 10 minutes, the mixture is respectively transferred into three cuvettes and is measured on a computer, the value displayed at the moment is the nitrogen content of the organic fertilizer, the detection of the organic matter content in the organic fertilizer is continued according to the method, and then the data is recorded.
In the first step, the length of the open-air reaction tank is 5 meters, the width is 3 meters, and the height is 1 meter.
In the step two, in the component A, the ratio of the biogas slurry to the water mixture is controlled to be 1: about 1.
In the third step, the preparation method of the probiotics and the fermentation mother bacteria comprises the following steps: taking out a certain dosage of probiotic strains and fermentation mother strains, and performing liquid anaerobic fermentation to form the probiotic and the fermentation mother strains.
In the fifth step, the component C is uniformly poured on the surface of the cotton waste residue and exceeds the surface of the cotton waste residue by 5-10 cm.
And step six, when the cotton waste residue and the C component in the open-air reaction tank are stirred, a telescopic stirrer is adopted to fully and uniformly stir the C component and the cotton waste residue, so that the cotton waste residue and the C component are prevented from being insufficiently fermented, and the finally prepared organic fertilizer is not qualified.
And seventhly, fermenting the carbon energy organic fertilizer into complete black, and grabbing the black by hands until the black is in a mycelium shape.
Example two
A preparation method of a probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing comprises the following steps:
the method comprises the following steps: opening a large reaction tank in the open air, and then pulling, transporting and filling the prepared cotton waste residues by a transport vehicle;
step two: directly watering the inside of an open reaction tank, then using a stirrer to fully mix water and cotton waste residues, after one month, pushing a carbon energy organic fertilizer formed after natural fermentation to an open flat ground by using a forklift, or preparing a mixture of biogas slurry and water with a certain dosage, or cattle and sheep excrement, and naming the materials as a component A;
step three: preparing a certain dosage of probiotics and zymocyte, and naming the probiotics and zymocyte as a component B;
step four: mixing the component A and the component B according to the proportion of 1: 0.2, stirring and mixing uniformly to form a component C;
step five: uniformly pouring the component C on the surface of cotton waste residue in an open-air reaction tank;
step six: stirring the cotton waste residue and the component C in the open-air reaction tank by using a stirrer, and repeatedly stirring for 2-3 times to ensure that the cotton waste residue and the component C in the open-air reaction tank naturally react to form a carbon-energy organic fertilizer;
step seven: after one month, the carbon energy organic fertilizer after the reaction is transported on an open flat ground by a forklift, the carbon energy organic fertilizer is piled up to a height of about 1.5 m, a layer of plastic cloth is wrapped on the surface of the organic fertilizer, and the organic fertilizer is formed through continuous fermentation;
step eight: weighing 0.5g of organic fertilizer sample, putting the organic fertilizer sample into a glass triangular flask, sequentially adding 5.0mL of concentrated sulfuric acid and 5-10 drops of organic fertilizer digestion accelerator, shaking up gently, putting a small bent-neck funnel on a bottle mouth, heating the mixture on an electric furnace at a low temperature for 10 minutes, observing, heating the mixture until the mixture is digested to white, taking down the mixture, and cooling the mixture to obtain a digestive juice;
step nine: carefully transferring the treated digestive juice into a 100mL volumetric flask by using distilled water, adding the distilled water to a scale mark for constant volume, shaking up, filtering into the triangular flask, discarding the initial filtrate if the triangular flask is not dry, sucking 2mL of the filtrate into the 100mL volumetric flask, keeping the constant volume by using the distilled water, and shaking up to obtain a solution to be detected;
step ten: and (3) respectively sucking distilled water by using a 5mL suction pipe to serve as blank liquid, and respectively adding 2.0mL of the fertilizer standard liquid and the liquid to be detected into three small glass bottles in sequence: 4 drops of fertilizer ammonium nitrogen No. 1 reagent, 4 drops of fertilizer ammonium nitrogen No. 2 reagent, 4 drops of fertilizer ammonium nitrogen No. 3 reagent and 2 drops of fertilizer ammonium nitrogen No. 4 reagent are shaken up, after standing for 10 minutes, the mixture is respectively transferred into three cuvettes and is measured on a computer, the value displayed at the moment is the nitrogen content of the organic fertilizer, the detection of the organic matter content in the organic fertilizer is continued according to the method, and then the data is recorded.
In the first step, the length of the open-air reaction tank is 5 meters, the width is 3 meters, and the height is 1 meter.
In the step two, in the component A, the ratio of the biogas slurry to the water mixture is controlled to be 1: about 1.
In the third step, the preparation method of the probiotics and the fermentation mother bacteria comprises the following steps: taking out a certain dosage of probiotic strains and fermentation mother strains, and performing liquid anaerobic fermentation to form the probiotic and the fermentation mother strains.
In the fifth step, the component C is uniformly poured on the surface of the cotton waste residue and exceeds the surface of the cotton waste residue by 5-10 cm.
And step six, when the cotton waste residue and the C component in the open-air reaction tank are stirred, a telescopic stirrer is adopted to fully and uniformly stir the C component and the cotton waste residue, so that the cotton waste residue and the C component are prevented from being insufficiently fermented, and the finally prepared organic fertilizer is not qualified.
And seventhly, fermenting the carbon energy organic fertilizer into complete black, and grabbing the black by hands until the black is in a mycelium shape.
EXAMPLE III
A preparation method of a probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing comprises the following steps:
the method comprises the following steps: opening a large reaction tank in the open air, and then pulling, transporting and filling the prepared cotton waste residues by a transport vehicle;
step two: directly watering the inside of an open reaction tank, then using a stirrer to fully mix water and cotton waste residues, after one month, pushing a carbon energy organic fertilizer formed after natural fermentation to an open flat ground by using a forklift, or preparing a mixture of biogas slurry and water with a certain dosage, or cattle and sheep excrement, and naming the materials as a component A;
step three: preparing a certain dosage of probiotics and zymocyte, and naming the probiotics and zymocyte as a component B;
step four: mixing the component A and the component B according to the proportion of 1: 0.3, stirring and mixing uniformly to form a component C;
step five: uniformly pouring the component C on the surface of cotton waste residue in an open-air reaction tank;
step six: stirring the cotton waste residue and the component C in the open-air reaction tank by using a stirrer, and repeatedly stirring for 2-3 times to ensure that the cotton waste residue and the component C in the open-air reaction tank naturally react to form a carbon-energy organic fertilizer;
step seven: after one month, the carbon energy organic fertilizer after the reaction is transported on an open flat ground by a forklift, the carbon energy organic fertilizer is piled up to a height of about 1.5 m, a layer of plastic cloth is wrapped on the surface of the organic fertilizer, and the organic fertilizer is formed through continuous fermentation;
step eight: weighing 0.5g of organic fertilizer sample, putting the organic fertilizer sample into a glass triangular flask, sequentially adding 5.0mL of concentrated sulfuric acid and 5-10 drops of organic fertilizer digestion accelerator, shaking up gently, putting a small bent-neck funnel on a bottle mouth, heating the mixture on an electric furnace at a low temperature for 10 minutes, observing, heating the mixture until the mixture is digested to white, taking down the mixture, and cooling the mixture to obtain a digestive juice;
step nine: carefully transferring the treated digestive juice into a 100mL volumetric flask by using distilled water, adding the distilled water to a scale mark for constant volume, shaking up, filtering into the triangular flask, discarding the initial filtrate if the triangular flask is not dry, sucking 2mL of the filtrate into the 100mL volumetric flask, keeping the constant volume by using the distilled water, and shaking up to obtain a solution to be detected;
step ten: and (3) respectively sucking distilled water by using a 5mL suction pipe to serve as blank liquid, and respectively adding 2.0mL of the fertilizer standard liquid and the liquid to be detected into three small glass bottles in sequence: 4 drops of fertilizer ammonium nitrogen No. 1 reagent, 4 drops of fertilizer ammonium nitrogen No. 2 reagent, 4 drops of fertilizer ammonium nitrogen No. 3 reagent and 2 drops of fertilizer ammonium nitrogen No. 4 reagent are shaken up, after standing for 10 minutes, the mixture is respectively transferred into three cuvettes and is measured on a computer, the value displayed at the moment is the nitrogen content of the organic fertilizer, the detection of the organic matter content in the organic fertilizer is continued according to the method, and then the data is recorded.
In the first step, the length of the open-air reaction tank is 5 meters, the width is 3 meters, and the height is 1 meter.
In the step two, in the component A, the ratio of the biogas slurry to the water mixture is controlled to be 1: about 1.
In the third step, the preparation method of the probiotics and the fermentation mother bacteria comprises the following steps: taking out a certain dosage of probiotic strains and fermentation mother strains, and performing liquid anaerobic fermentation to form the probiotic and the fermentation mother strains.
In the fifth step, the component C is uniformly poured on the surface of the cotton waste residue and exceeds the surface of the cotton waste residue by 5-10 cm.
And step six, when the cotton waste residue and the C component in the open-air reaction tank are stirred, a telescopic stirrer is adopted to fully and uniformly stir the C component and the cotton waste residue, so that the cotton waste residue and the C component are prevented from being insufficiently fermented, and the finally prepared organic fertilizer is not qualified.
And seventhly, fermenting the carbon energy organic fertilizer into complete black, and grabbing the black by hands until the black is in a mycelium shape.
Example four
A preparation method of a probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing comprises the following steps:
the method comprises the following steps: opening a large reaction tank in the open air, and then pulling, transporting and filling the prepared cotton waste residues by a transport vehicle;
step two: directly watering the inside of an open reaction tank, then using a stirrer to fully mix water and cotton waste residues, after one month, pushing a carbon energy organic fertilizer formed after natural fermentation to an open flat ground by using a forklift, or preparing a mixture of biogas slurry and water with a certain dosage, or cattle and sheep excrement, and naming the materials as a component A;
step three: preparing a certain dosage of probiotics and zymocyte, and naming the probiotics and zymocyte as a component B;
step four: mixing the component A and the component B according to the proportion of 1: 0.4, stirring and mixing uniformly to form a component C;
step five: uniformly pouring the component C on the surface of cotton waste residue in an open-air reaction tank;
step six: stirring the cotton waste residue and the component C in the open-air reaction tank by using a stirrer, and repeatedly stirring for 2-3 times to ensure that the cotton waste residue and the component C in the open-air reaction tank naturally react to form a carbon-energy organic fertilizer;
step seven: after one month, the carbon energy organic fertilizer after the reaction is transported on an open flat ground by a forklift, the carbon energy organic fertilizer is piled up to a height of about 1.5 m, a layer of plastic cloth is wrapped on the surface of the organic fertilizer, and the organic fertilizer is formed through continuous fermentation;
step eight: weighing 0.5g of organic fertilizer sample, putting the organic fertilizer sample into a glass triangular flask, sequentially adding 5.0mL of concentrated sulfuric acid and 5-10 drops of organic fertilizer digestion accelerator, shaking up gently, putting a small bent-neck funnel on a bottle mouth, heating the mixture on an electric furnace at a low temperature for 10 minutes, observing, heating the mixture until the mixture is digested to white, taking down the mixture, and cooling the mixture to obtain a digestive juice;
step nine: carefully transferring the treated digestive juice into a 100mL volumetric flask by using distilled water, adding the distilled water to a scale mark for constant volume, shaking up, filtering into the triangular flask, discarding the initial filtrate if the triangular flask is not dry, sucking 2mL of the filtrate into the 100mL volumetric flask, keeping the constant volume by using the distilled water, and shaking up to obtain a solution to be detected;
step ten: and (3) respectively sucking distilled water by using a 5mL suction pipe to serve as blank liquid, and respectively adding 2.0mL of the fertilizer standard liquid and the liquid to be detected into three small glass bottles in sequence: 4 drops of fertilizer ammonium nitrogen No. 1 reagent, 4 drops of fertilizer ammonium nitrogen No. 2 reagent, 4 drops of fertilizer ammonium nitrogen No. 3 reagent and 2 drops of fertilizer ammonium nitrogen No. 4 reagent are shaken up, after standing for 10 minutes, the mixture is respectively transferred into three cuvettes and is measured on a computer, the value displayed at the moment is the nitrogen content of the organic fertilizer, the detection of the organic matter content in the organic fertilizer is continued according to the method, and then the data is recorded.
In the first step, the length of the open-air reaction tank is 5 meters, the width is 3 meters, and the height is 1 meter.
In the step two, in the component A, the ratio of the biogas slurry to the water mixture is controlled to be 1: about 1.
In the third step, the preparation method of the probiotics and the fermentation mother bacteria comprises the following steps: taking out a certain dosage of probiotic strains and fermentation mother strains, and performing liquid anaerobic fermentation to form the probiotic and the fermentation mother strains.
In the fifth step, the component C is uniformly poured on the surface of the cotton waste residue and exceeds the surface of the cotton waste residue by 5-10 cm.
And step six, when the cotton waste residue and the C component in the open-air reaction tank are stirred, a telescopic stirrer is adopted to fully and uniformly stir the C component and the cotton waste residue, so that the cotton waste residue and the C component are prevented from being insufficiently fermented, and the finally prepared organic fertilizer is not qualified.
And seventhly, fermenting the carbon energy organic fertilizer into complete black, and grabbing the black by hands until the black is in a mycelium shape.
EXAMPLE five
A preparation method of a probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing comprises the following steps:
the method comprises the following steps: opening a large reaction tank in the open air, and then pulling, transporting and filling the prepared cotton waste residues by a transport vehicle;
step two: directly watering the inside of an open reaction tank, then using a stirrer to fully mix water and cotton waste residues, after one month, pushing a carbon energy organic fertilizer formed after natural fermentation to an open flat ground by using a forklift, or preparing a mixture of biogas slurry and water with a certain dosage, or cattle and sheep excrement, and naming the materials as a component A;
step three: preparing a certain dosage of probiotics and zymocyte, and naming the probiotics and zymocyte as a component B;
step four: mixing the component A and the component B according to the proportion of 1: 0.5, stirring and mixing uniformly to form a component C;
step five: uniformly pouring the component C on the surface of cotton waste residue in an open-air reaction tank;
step six: stirring the cotton waste residue and the component C in the open-air reaction tank by using a stirrer, and repeatedly stirring for 2-3 times to ensure that the cotton waste residue and the component C in the open-air reaction tank naturally react to form a carbon-energy organic fertilizer;
step seven: after one month, the carbon energy organic fertilizer after the reaction is transported on an open flat ground by a forklift, the carbon energy organic fertilizer is piled up to a height of about 1.5 m, a layer of plastic cloth is wrapped on the surface of the organic fertilizer, and the organic fertilizer is formed through continuous fermentation;
step eight: weighing 0.5g of organic fertilizer sample, putting the organic fertilizer sample into a glass triangular flask, sequentially adding 5.0mL of concentrated sulfuric acid and 5-10 drops of organic fertilizer digestion accelerator, shaking up gently, putting a small bent-neck funnel on a bottle mouth, heating the mixture on an electric furnace at a low temperature for 10 minutes, observing, heating the mixture until the mixture is digested to white, taking down the mixture, and cooling the mixture to obtain a digestive juice;
step nine: carefully transferring the treated digestive juice into a 100mL volumetric flask by using distilled water, adding the distilled water to a scale mark for constant volume, shaking up, filtering into the triangular flask, discarding the initial filtrate if the triangular flask is not dry, sucking 2mL of the filtrate into the 100mL volumetric flask, keeping the constant volume by using the distilled water, and shaking up to obtain a solution to be detected;
step ten: and (3) respectively sucking distilled water by using a 5mL suction pipe to serve as blank liquid, and respectively adding 2.0mL of the fertilizer standard liquid and the liquid to be detected into three small glass bottles in sequence: 4 drops of fertilizer ammonium nitrogen No. 1 reagent, 4 drops of fertilizer ammonium nitrogen No. 2 reagent, 4 drops of fertilizer ammonium nitrogen No. 3 reagent and 2 drops of fertilizer ammonium nitrogen No. 4 reagent are shaken up, after standing for 10 minutes, the mixture is respectively transferred into three cuvettes and is measured on a computer, the value displayed at the moment is the nitrogen content of the organic fertilizer, the detection of the organic matter content in the organic fertilizer is continued according to the method, and then the data is recorded.
In the first step, the length of the open-air reaction tank is 5 meters, the width is 3 meters, and the height is 1 meter.
In the step two, in the component A, the ratio of the biogas slurry to the water mixture is controlled to be 1: about 1.
In the third step, the preparation method of the probiotics and the fermentation mother bacteria comprises the following steps: taking out a certain dosage of probiotic strains and fermentation mother strains, and performing liquid anaerobic fermentation to form the probiotic and the fermentation mother strains.
In the fifth step, the component C is uniformly poured on the surface of the cotton waste residue and exceeds the surface of the cotton waste residue by 5-10 cm.
And step six, when the cotton waste residue and the C component in the open-air reaction tank are stirred, a telescopic stirrer is adopted to fully and uniformly stir the C component and the cotton waste residue, so that the cotton waste residue and the C component are prevented from being insufficiently fermented, and the finally prepared organic fertilizer is not qualified.
And seventhly, fermenting the carbon energy organic fertilizer into complete black, and grabbing the black by hands until the black is in a mycelium shape.
In summary, in the fourth step, when the content ratio of the component A to the component B is 1: when 0.4, the quality of the carbon energy organic fertilizer is optimal, the nitrogen content in the carbon energy organic fertilizer is 6%, and the organic matter content is 46%.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A preparation method of a probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing is characterized by comprising the following steps: the method comprises the following steps:
the method comprises the following steps: opening a large reaction tank in the open air, and then pulling, transporting and filling the prepared cotton waste residues by a transport vehicle;
step two: directly watering the inside of an open reaction tank, then using a stirrer to fully mix water and cotton waste residues, after one month, pushing a carbon energy organic fertilizer formed after natural fermentation to an open flat ground by using a forklift, or preparing a mixture of biogas slurry and water with a certain dosage, or cattle and sheep excrement, and naming the materials as a component A;
step three: preparing a certain dosage of probiotics and zymocyte, and naming the probiotics and zymocyte as a component B;
step four: mixing the component A and the component B according to the proportion of 1: 0.1-0.5, and then evenly stirring and mixing to form a component C;
step five: uniformly pouring the component C on the surface of cotton waste residue in an open-air reaction tank;
step six: stirring the cotton waste residue and the component C in the open-air reaction tank by using a stirrer, and repeatedly stirring for 2-3 times to ensure that the cotton waste residue and the component C in the open-air reaction tank naturally react to form a carbon-energy organic fertilizer;
step seven: after one month, the carbon energy organic fertilizer after the reaction is transported on an open flat ground by a forklift, the carbon energy organic fertilizer is piled up to a height of about 1.5 m, a layer of plastic cloth is wrapped on the surface of the organic fertilizer, and the organic fertilizer is formed through continuous fermentation;
step eight: weighing 0.5g of organic fertilizer sample, putting the organic fertilizer sample into a glass triangular flask, sequentially adding 5.0mL of concentrated sulfuric acid and 5-10 drops of organic fertilizer digestion accelerator, shaking up gently, putting a small bent-neck funnel on a bottle mouth, heating the mixture on an electric furnace at a low temperature for 10 minutes, observing, heating the mixture until the mixture is digested to white, taking down the mixture, and cooling the mixture to obtain a digestive juice;
step nine: carefully transferring the treated digestive juice into a 100mL volumetric flask by using distilled water, adding the distilled water to a scale mark for constant volume, shaking up, filtering into the triangular flask, discarding the initial filtrate if the triangular flask is not dry, sucking 2mL of the filtrate into the 100mL volumetric flask, keeping the constant volume by using the distilled water, and shaking up to obtain a solution to be detected;
step ten: and (3) respectively sucking distilled water by using a 5mL suction pipe to serve as blank liquid, and respectively adding 2.0mL of the fertilizer standard liquid and the liquid to be detected into three small glass bottles in sequence: 4 drops of fertilizer ammonium nitrogen No. 1 reagent, 4 drops of fertilizer ammonium nitrogen No. 2 reagent, 4 drops of fertilizer ammonium nitrogen No. 3 reagent and 2 drops of fertilizer ammonium nitrogen No. 4 reagent are shaken up, after standing for 10 minutes, the mixture is respectively transferred into three cuvettes and is measured on a computer, the value displayed at the moment is the nitrogen content of the organic fertilizer, the detection of the organic matter content in the organic fertilizer is continued according to the method, and then the data is recorded.
2. The preparation method of the probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing according to claim 1, characterized by comprising the following steps: in the first step, the length of the open-air reaction tank is 5 meters, the width of the open-air reaction tank is 3 meters, and the height of the open-air reaction tank is 1 meter.
3. The preparation method of the probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing according to claim 1, characterized by comprising the following steps: in the step two, in the component A, the ratio of the biogas slurry to the water mixture is controlled to be 1: about 1.
4. The preparation method of the probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing according to claim 1, characterized by comprising the following steps: in the third step, the preparation method of the probiotics and the fermentation mother bacteria comprises the following steps: taking out a certain dosage of probiotic strains and fermentation mother strains, and performing liquid anaerobic fermentation to form the probiotic and the fermentation mother strains.
5. The preparation method of the probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing according to claim 1, characterized by comprising the following steps: in the fifth step, the component C is uniformly poured on the surface of the cotton waste residue and exceeds the surface of the cotton waste residue by 5-10 cm.
6. The preparation method of the probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing according to claim 1, characterized by comprising the following steps: and in the sixth step, when the cotton waste residue and the C component in the open-air reaction tank are stirred, the C component and the cotton waste residue are fully and uniformly stirred by adopting a telescopic stirrer, so that the problem that the finally prepared organic fertilizer is unqualified due to insufficient fermentation of the cotton waste residue and the C component is solved.
7. The preparation method of the probiotic fermented carbon-energy organic fertilizer based on cotton waste residue mixing according to claim 1, characterized by comprising the following steps: and seventhly, when the carbon energy organic fertilizer is fermented to be completely black and is grabbed by hands, all the carbon energy organic fertilizer is in a mycelium shape.
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