CN104798485B - A kind of method promoting Rhizoma Anemarrhenae seed to sprout - Google Patents
A kind of method promoting Rhizoma Anemarrhenae seed to sprout Download PDFInfo
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- CN104798485B CN104798485B CN201510183629.9A CN201510183629A CN104798485B CN 104798485 B CN104798485 B CN 104798485B CN 201510183629 A CN201510183629 A CN 201510183629A CN 104798485 B CN104798485 B CN 104798485B
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- seed
- rhizoma anemarrhenae
- growth regulator
- plant growth
- dry distillation
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Abstract
The invention discloses a kind of method promoting Rhizoma Anemarrhenae seed to sprout, step is：Seed sprout before, with plant growth regulator, Rhizoma Anemarrhenae seed is carried out with pretreatment, described plant growth regulator be by mass ratio be 1～13:1～2:1～3 Semen Crataegi, jujube nucleus and walnut shell carry out the dry distillation liquid that dry distilling obtains.The present invention carries out pretreatment as plant growth regulator to Rhizoma Anemarrhenae seed using plant dry distillation liquid, and preprocess method is simply it is easy to operation, pollution-free, can significantly improve the germination rate of Rhizoma Anemarrhenae seed, high financial profit.And plant dry distillation liquid used be Semen Crataegi, walnut shell, the core of these three plants of jujube nucleus, shell carry out the dry distillation liquid that dry distilling obtains, its preparation method is simple to operate, succinctly quick, it is easily achieved, raw materials used for plant refuse it is achieved that making full use of of waste, turn waste into wealth, low cost, is easy to industrialized production.
Description
Technical field
The present invention relates to a kind of method promoting Rhizoma Anemarrhenae seed to sprout is and in particular to one kind extracts using from plant
Dry distillation liquid come to promote Rhizoma Anemarrhenae seed sprout in method, belong to seed processing technology field.
Background technology
With being filled with of " back to nature " trend, traditional natural plant is increasingly favored by people.The Rhizoma Anemarrhenae is hundred
The dry rhizome of conjunction section plant Rhizoma Anemarrhenae Anemarrhena asphodeloides Bge., have clearing away heat-fire, nourshing Yin and drynsessmoistening prescription and
Quench the thirst relieving restlessness the effects such as.Domestic and international market increasingly increases to the demand of rhizoma ane marrhenae at present, and production scale and cultivated area are not
Disconnected expansion.Seed is the source of Producing medicinal herbs, is related to the yield of medical material and quality in the future.For improving seed germination rate, raw
How seed is carried out using growth regulators such as gibberellins on product and sprout pre-treatment, gibberellins are synthetic, there is high cost, dirt
The approach that the problems such as contaminate serious, therefore searching effectively facilitate the sprouting of Rhizoma Anemarrhenae seed is more and more heavier for the yield improving rhizoma ane marrhenae
Will.
Dry distillation liquid refers to be put into vegetable material the flue gas natural cooling liquefaction emerge in heating carbonization process in retort
The concentrated liquid obtaining.In recent years, the medical and edibility of dry distillation liquid is taken seriously, and is widely used in sterilization, disease prevention, food
The fields such as product are anti-corrosion, seasoning, fruit freshness preserving.It is rarely reported however, dry distillation liquid being used for Chinese crude drug seed and sprouting.
Content of the invention
It is an object of the invention to provide a kind of method promoting Rhizoma Anemarrhenae seed to sprout, the method is using plant dry distillation liquid as plant
Thing growth regulator carries out pretreatment to Rhizoma Anemarrhenae seed, can significantly improve Rhizoma Anemarrhenae seed and sprout, and improves Rhizoma Anemarrhenae yield on producing
Significant.
At present, dry distillation liquid is used in medicine mainly as sterilization, antipathogenic composition, has no in promoting Chinese crude drug seed to sprout
Report.Inventor finds under study for action, dry distillation liquid can be remarkably promoted Rhizoma Anemarrhenae seed as plant growth regulator and sprout, and
From the experimental results, the facilitation that the dry distillation liquid of different plant origins is sprouted to Rhizoma Anemarrhenae seed is different.Inventor passes through
A large amount of screening experiment, have finally given the dry distillation liquid that can effectively facilitate the sprouting of Rhizoma Anemarrhenae seed, and specifically have studied this dry distillation liquid
Preparation method and using method, thus be to be used for promoting the sprouting of Rhizoma Anemarrhenae seed to provide important technical support by dry distillation liquid.
Concrete technical scheme of the present invention is as follows：
A kind of method promoting Rhizoma Anemarrhenae seed to sprout, is characterized in that：Before seed is sprouted, with plant growth regulator to knowing
Parent species carries out pretreatment, described plant growth regulator be by mass ratio be 1～13:1～2:1～3 Semen Crataegi, jujube nucleus
Carry out the dry distillation liquid that dry distilling obtains with walnut shell, this dry distillation liquid is referred to as plant dry distillation liquid.
Plant growth regulator used by the present invention is a kind of plant dry distillation liquid, and it is preferably mass ratio is 11:2:2 or 13:
1:1 Semen Crataegi, jujube nucleus and walnut shell carry out the dry distillation liquid that dry distilling obtains.
Plant growth regulator used by the present invention, prepares in accordance with the following methods：By Semen Crataegi, jujube nucleus and walnut shell
By proportioning mixing, carry out dry distilling at 140～145 DEG C, 240～245 DEG C, 300～360 DEG C respectively, at a temperature of merging this three sections
The dry distillation liquid of gained, the dry distillation liquid remove impurity after merging obtains plant growth regulator.
When preparing plant growth regulator, it is incubated 1-2h at 140～145 DEG C, be incubated 2-3 hour at 240～245 DEG C,
300～360 DEG C of insulation 1-2 hours.
When preparing plant growth regulator, the dry distillation liquid after merging adopts following method remove impurity：By the dry distillation liquid after merging
Stir, stratification, take supernatant；Supernatant is distilled, gained distillate is filtered with after activated carbon process, gained filtrate
It is plant dry distillation liquid.
During dry distillation liquid remove impurity, supernatant is in 100～105 DEG C of air-distillations.
During dry distillation liquid remove impurity, supernatant distillation time is 2h.
During dry distillation liquid remove impurity, distillate adds the activated carbon of its quality 10%.
During dry distillation liquid remove impurity, activated carbon is processed 45 minutes at 95 DEG C, carries out remove impurity.
The present invention promotes, in the method that Rhizoma Anemarrhenae seed is sprouted, with plant growth regulator, seed to be carried out with the process of pretreatment
For：By plant growth regulator and water according to 1:The volume ratio mixing of 500-2000, obtains preprocessing solution, by preprocessing solution
It is sprayed at Rhizoma Anemarrhenae the surface of the seed, pretreatment is carried out to Rhizoma Anemarrhenae seed.
The present invention promotes in the method that Rhizoma Anemarrhenae seed is sprouted, and plant growth regulator is preferably 1 with the volume ratio of water:
1000.
The present invention promotes in the method that Rhizoma Anemarrhenae seed is sprouted, and during Rhizoma Anemarrhenae Seeds preprocess, the every 4h of preprocessing solution sprays one
Secondary, continuously spray 48h.
The present invention promotes in the method that Rhizoma Anemarrhenae seed is sprouted, and during Rhizoma Anemarrhenae Seeds preprocess, sprays preprocessing solution to drench
Plant skin to be advisable.
The present invention promotes in the method that Rhizoma Anemarrhenae seed is sprouted, and selects the Rhizoma Anemarrhenae seed of full seed health to be sprouted.
The present invention promotes, in the method that Rhizoma Anemarrhenae seed is sprouted, first Rhizoma Anemarrhenae seed to be carried out disinfection with liquor natrii hypochloritises, so
Rinsed well with water afterwards, then with plant growth regulator, pretreatment is carried out to Rhizoma Anemarrhenae seed.Liquor natrii hypochloritises' concentration used
Can be 0.525g L-1, sterilization 10min about.
According to the method described above seed is carried out after pretreatment, seed is placed in the damp sand of water content 60% and sprouts, temperature：
25 DEG C/20 DEG C (day night)；Illumination：14h light/10h is dark, and light intensity is 260-270 μm of ol/ (m2·s)；Relative humidity：65-70%.
After tested, the Rhizoma Anemarrhenae seed through pretreatment significantly improves than the not germination rate through pretreatment.
The present invention carries out pretreatment, preprocess method as plant growth regulator to Rhizoma Anemarrhenae seed using plant dry distillation liquid
Simply it is easy to operation, pollution-free, the germination rate of Rhizoma Anemarrhenae seed, high financial profit can be significantly improved.And plant dry distillation liquid used
Carry out the dry distillation liquid that dry distilling obtains, its preparation method operation letter for Semen Crataegi, walnut shell, the core of these three plants of jujube nucleus, shell
Single, succinctly quickly, it is easy to accomplish, raw materials used for plant refuse it is achieved that making full use of of waste, turn waste into wealth, cost
Low, it is easy to industrialized production.
Specific embodiment
The present invention will be further elaborated below by specific embodiment it should be appreciated that, the description below is only
In order to explain the present invention, its content is not limited.
The preparation of plant growth regulator
Embodiment 1
Take Semen Crataegi, jujube nucleus and walnut shell 1.5kg respectively, with mass ratio for 1:1:1 addition retort, is heated to 140
～145 DEG C, it is incubated 1 hour, continues to be heated to 240～245 DEG C, be incubated 2 hours, then temperature rises to 300～360 DEG C and maintains 1
Hour.Collect the dry distillation liquid of day part, mix and blend 30 minutes, proceed in separatory funnel and stand 12 hours, separate supernatant.Will
Supernatant adds distillating still, distills 2 hours in 100～105 DEG C, collects distillate.Distillate adds the activated carbon of 10wt%, stirs
Mix and be heated to 95 DEG C, maintain 45 minutes, filter, collect filtrate.Filtrate be the present invention plant growth regulator it is also possible to
Referred to as plant dry distillation liquid, similarly hereinafter.
Embodiment 2
Semen Crataegi, jujube nucleus and walnut shell is taken to amount to 1.5kg, mass ratio is 6:2:3 addition retorts, it is heated to 140～
145 DEG C, insulation 1 hour, continue to be heated to 240～245 DEG C, be incubated 2 hours, then temperature rises to 300～360 DEG C and remains 1 little
When.Collect the dry distillation liquid of day part, mix and blend 30 minutes, proceed in separatory funnel and stand 12 hours, separate supernatant.Will be upper
Clear liquid adds distillating still, distills 2 hours in 100～105 DEG C, collects distillate.Distillate adds the activated carbon of 10wt%, stirring
It is heated to 95 DEG C, maintains 45 minutes, filter, collect filtrate.Filtrate is the plant growth regulator of the present invention.
Embodiment 3
Semen Crataegi, jujube nucleus and walnut shell is taken to amount to 1.5kg, mass ratio is 11:2:2 addition retorts, it is heated to 140～
145 DEG C, insulation 1 hour, continue to be heated to 240～245 DEG C, be incubated 2 hours, then temperature rises to 300～360 DEG C and remains 1 little
When.Collect the dry distillation liquid of day part, mix and blend 30 minutes, proceed in separatory funnel and stand 12 hours, separate supernatant.Will be upper
Clear liquid adds distillating still, distills 2 hours in 100～105 DEG C, collects distillate.Distillate adds the activated carbon of 10wt%, stirring
It is heated to 95 DEG C, maintains 45 minutes, filter, collect filtrate.Filtrate is the plant growth regulator of the present invention.
Embodiment 4
Semen Crataegi, jujube nucleus and walnut shell is taken to amount to 1.5kg, mass ratio is 13:1:1 addition retort, it is heated to 140～
145 DEG C, insulation 1 hour, continue to be heated to 240～245 DEG C, be incubated 2 hours, then temperature rises to 300～360 DEG C and remains 1 little
When.Collect the dry distillation liquid of day part, mix and blend 30 minutes, proceed in separatory funnel and stand 12 hours, separate supernatant.Will be upper
Clear liquid adds distillating still, distills 2 hours in 100～105 DEG C, collects distillate.Distillate adds the activated carbon of 10wt%, stirring
It is heated to 95 DEG C, maintains 45 minutes, filter, collect filtrate.Filtrate is the plant growth regulator of the present invention.
Comparative example 1
Method according to embodiment 3 prepares plant growth regulator, and except for the difference that, plant source used is：Quercus acutissima Carr.
(Magnolia denudata), Chinese scholar tree (Sophora japonica), yellow rose (Rosa xanthina Lindl) these three
The trunk of plant, their mass ratio is 1:1:1.
Comparative example 2
Method according to embodiment 3 prepares plant growth regulator, and except for the difference that, plant source used is：Cercis
(Cercis chinensin Bunge), Salix babylonica L. (Salix babylonica), these three plants of chinaberry (Melia azedarach)
The trunk of thing, their mass ratio is 1:1:1.
The application examples of plant growth regulator treatment Rhizoma Anemarrhenae seed
1st, choose the Rhizoma Anemarrhenae seed of full seed health, use 0.525g L-1Hypochlorite disinfectant 10min, then with distillation
Water rinses 3 times.
2nd, prepare preprocessing solution：By above-described embodiment and comparative example preparation plant growth regulator individually with water
According to 1:500、1:1000 and 1:2000 volume ratio mixing, is made into different preprocessing solutions.
3rd, the pretreatment of Rhizoma Anemarrhenae seed：The preprocessing solution of preparation is sprayed on respectively the Rhizoma Anemarrhenae the surface of the seed disinfecting, with
Drench kind of a skin to be advisable, every 4h sprays once, uninterruptedly sprays, carry out the blank being sprayed using pure water simultaneously in 48h,
Each processes 6 repetitions.
4th, seed is sprouted：Pretreated Rhizoma Anemarrhenae seed is placed in the damp sand of water content 60% and sprouts, temperature：25℃/
20 DEG C (day night)；Illumination：14h light/10h is dark, and light intensity is 260-270 μm of ol/ (m2·s)；Relative humidity：65-70%.Daily
Record seed is sprouted in quantity (dash forward to break in the seed coat by radicle and be defined) germination process and is kept the skin wet in time needed for guarantee that seed is sprouted
The moisture wanted.25d terminates to sprout experiment, measures all seedling root length alive, fresh weight, counts germination rate, measures amylase activity (enzyme
Activity is higher, and germination rate is higher).The mensure of amylase activity：Amylase activity adopts ultraviolet method, is become with OD value size per minute
Change and represent diastatic relative activity.
After each group Rhizoma Anemarrhenae seed is cultivated 25 days according to the method described above, each index is as follows：
Table 1 each group Rhizoma Anemarrhenae seed germination rate and growth of seedling situation
Can be seen that plant growth regulator and the blank of the embodiment of the present invention 1,2,3,4 from the data of above-mentioned table 1
Compare the germination rate of raising Rhizoma Anemarrhenae seed that all can be different degrees of, seed starches enzymatic activity, fresh weight and radicle length, and pass through him
Between contrast can be seen that embodiment 3 and the facilitation of embodiment 4 is more significant, embodiment 1,2 is taken second place.By this
Bright plant growth regulator can be seen that the plant growth regulating used by the present invention with the contrast of comparative example plant growth regulator
Agent can significantly improve the germination rate of Rhizoma Anemarrhenae seed, seed starches enzymatic activity, fresh weight and radicle length.
Claims (9)
1. a kind of method promoting Rhizoma Anemarrhenae seed to sprout, is characterized in that：Before seed is sprouted, with plant growth regulator to the Rhizoma Anemarrhenae
Seed carries out pretreatment, described plant growth regulator be by mass ratio be 1～13:1～2:1～3 Semen Crataegi, jujube nucleus and
Walnut shell carries out the dry distillation liquid that dry distilling obtains；
With the process that plant growth regulator carries out pretreatment to seed it is：By plant growth regulator and water according to 1:500-
2000 volume ratio mixing, obtains preprocessing solution, preprocessing solution is sprayed at Rhizoma Anemarrhenae the surface of the seed, Rhizoma Anemarrhenae seed is carried out
Pretreatment.
2. method according to claim 1, is characterized in that：The mass ratio 11 of Semen Crataegi, jujube nucleus and walnut shell:2:2 or
13:1:1.
3. method according to claim 1, is characterized in that：During Rhizoma Anemarrhenae Seeds preprocess, the every 4h of preprocessing solution sprays one
Secondary, continuously spray 48h.
4. method according to claim 1, is characterized in that：During Rhizoma Anemarrhenae Seeds preprocess, spray preprocessing solution to drench
Plant skin to be advisable.
5. the method according to claim 1,2 or 3, is characterized in that：With plant growth regulator, Rhizoma Anemarrhenae seed is carried out pre-
Rhizoma Anemarrhenae seed is first carried out disinfection by before processing with liquor natrii hypochloritises, is then rinsed well with water.
6. the method according to claim 1,2 or 3, is characterized in that：The preparation method of plant growth regulator is：By Fructus Crataegi
Core, jujube nucleus and walnut shell are pressed proportioning and are mixed, and carry out dry distilling respectively at 140～145 DEG C, 240～245 DEG C, 300～360 DEG C,
Merge the dry distillation liquid of gained at a temperature of this three sections, the dry distillation liquid remove impurity after merging obtains plant growth regulator.
7. method according to claim 6, is characterized in that：When preparing plant growth regulator, it is incubated at 140～145 DEG C
1-2h, is incubated 2-3 hour at 240～245 DEG C, is incubated 1-2 hour at 300～360 DEG C.
8. method according to claim 6, is characterized in that：When preparing plant growth regulator, the dry distillation liquid after merging is adopted
With following method remove impurity：By merge after dry distillation liquid stir, stratification, take supernatant；Supernatant is distilled, gained steams
Distillate is filtered with after activated carbon process, and gained filtrate is plant growth regulator.
9. method according to claim 8, is characterized in that：When preparing plant growth regulator, supernatant is 100～105
DEG C air-distillation 2h；Distillate adds the activated carbon of its quality 10% to process 45 minutes at 95 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510183629.9A CN104798485B (en) | 2015-04-17 | 2015-04-17 | A kind of method promoting Rhizoma Anemarrhenae seed to sprout |
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| CN201510183629.9A CN104798485B (en) | 2015-04-17 | 2015-04-17 | A kind of method promoting Rhizoma Anemarrhenae seed to sprout |
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| CN104798485B true CN104798485B (en) | 2017-03-01 |
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Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4702677B2 (en) * | 2003-12-19 | 2011-06-15 | 大幸Ｔｅｃ株式会社 | Seed disinfectant |
| CN101273730A (en) * | 2008-05-16 | 2008-10-01 | 南京林业大学 | Ginko pyroligneous and its applications in promoting germination of crop seeds and growth of seedlings |
| CN103416191A (en) * | 2013-07-25 | 2013-12-04 | 王运海 | Method for planting rhizoma anemarrhenae |
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