CN107668051A - A kind of preparation for promoting grafting wound healing - Google Patents
A kind of preparation for promoting grafting wound healing Download PDFInfo
- Publication number
- CN107668051A CN107668051A CN201711235624.1A CN201711235624A CN107668051A CN 107668051 A CN107668051 A CN 107668051A CN 201711235624 A CN201711235624 A CN 201711235624A CN 107668051 A CN107668051 A CN 107668051A
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- CN
- China
- Prior art keywords
- preparation
- glucosinolate
- wound healing
- zeatin
- grafting
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of preparation for promoting grafting wound healing, it is more particularly to a kind of by the use of glucosinolate bactericide, zeatin and sucrose according to the consolidant after certain proportioning dilution as grafting wound.After first zeatin being dissolved in the ethanol that volumetric concentration is 95%, the Tween 80 that volumetric concentration is 98% is added, is well mixed, solution 1 is made；Solution 1 and glucosinolate are mixed together uniformly again, sucrose is then added, stirs, be 1L with water constant volume.The preparation of the present invention can promote grafting wound healing, improve wound healing rate, shorten healing time.
Description
Technical field
The invention belongs to vegetable grafting technology, is related to a kind of preparation for promoting grafting wound healing.
Background technology
Grafting is the effective measures for solving soil-borne disease such as droop and bacterial wilt, and the quality of Relationship between Graft Effect depends on stock
With the compatibility of scion, depending on stock and the healing of scion wound.The agglutination of stock and scion wound includes
The forming layer of anvil fringe section touches mutually, afterwards both produce callus respectively, callus is combined, through cell division,
Break up and connect forming layer, form callus completely.Specifically include section and form downright bad layer, activated by cytoplasm
And causing the contiguity between the accumulation of golgiosome and anvil fringe, anvil, fringe Callus formation and downright bad layer disappear, vascular bundle between anvil fringe
Differentiation, graft union and survive.
Glucosinolate is a kind of Secondary metabolites for being rich in sulphur in brassicaceous vegetable, can by R base differences
It is divided into fats glucosinolate, indoles glucosinolate and aromatics glucosinolate.Its catabolite isothiocyanate
（ITCs）Degraded with the antitumaous effect to attract people's attention, particularly fats glucosinolate 4- methoxies sulphur oxygen-butyl glucosinolate
Product sulforaphen, the activity of stage I enzyme can be suppressed and promote the detoxicating activity of the enzyme of stage II, so as to play antitumaous effect.
In addition, the metabolite of 2- allyl isothiocyanates glycosides and indyl -3- methyl-mustard oil glycosides also has been shown to have active anticancer.
Glucosinolate in cabbage mustard is based on fats glucosinolate, and wherein 3- cyclobutenyls glucosinolate content is most.
Alternaric bacteria, rape pink mold can also substantially be suppressed by applying the different thio nitrile hydrochlorate of glucosinolate metabolite in vitro
With the growth of the multiple pathogenic microorganisms such as Fusarium oxysporum.Overexpression jowarCYP79A2After gene, aromatics glucosinolate contains
Amount significantly rise, its resistance to pseudomonad are also strengthened.Thus glucosinolate and its metabolite resist food in plant
Played an important role in the defense response of careless animal, insect and pathogenic microorganism, be the important of plant congenital immunity reaction
Part, while there is also a glucosinolate metabolic pathway activated by active somatic cell in itself in plant, in fungi
Played a significant role in resistance.
The application to be focused mostly on for the document of grafting wound healing in plant hormone and bactericide, but ignores Plant Secondary Materials
The application of metabolin.There is fungi, the brassicaceous vegetable metabolin of bacterial resistance, at hormone so that glucosinolate is this kind of
Reason, it is possible to achieve grafting wound preferably heals.
The content of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of system for promoting vegetable grafting wound healing
Agent.Said preparation is mainly combined with glucosinolate, zeatin and sucrose, can promote grafting wound healing, improves wound healing rate,
Shorten healing time.
For achieving the above object, the present invention adopts the following technical scheme that：
A kind of preparation for grafting wound healing, said preparation in terms of 1L, consisting of：2 μM ~ 100 μM of glucosinolate, it is beautiful
1 μM ~ 10 μM, sucrose 1wt% ~ 10wt% of rice element, volumetric concentration are 95% ethanol 50mL ~ 125mL, and volumetric concentration is 98% tween
80 11.1mL ~ 67.2mL, surplus are water.
Preferably, said preparation is in terms of 1L, consisting of：50 μM of glucosinolate, 3 μM, sucrose 3wt% of zeatin, volumetric concentration
For 95% ethanol 50mL, volumetric concentration is 98% Tween 80 15mL, and surplus is water.
A kind of method for preparing the preparation as described above for grafting wound healing：Zeatin is first dissolved in body
After product concentration is 95% ethanol, the Tween 80 that volumetric concentration is 98% is added, is well mixed, solution 1 is made；Again solution 1 with
Glucosinolate is mixed together uniformly, then adds sucrose, is stirred, and is 1L with water constant volume.
The application to said preparation of illustrating below illustrates：Take tomato stock and each 500 of ingraftment seed, plant to be planted seedling
Grow to two leaves wholeheartedly, three leaves wholeheartedly, four leaves wholeheartedly when carry out grafting respectively, sprayed respectively using said preparation, it is outer painting and
The libation at an ancient wedding ceremony is stained with the wound of scion and stock, is clamped afterwards with Grafting clip, under 85%-90% air humidity, dark 25 degree of lower cultures
After 3d, solar protection devices is gradually removed, increases light intensity, holding air humidity is 75%-80%, and greenhouse is placed in after wound completely healing
In cultivated.
Glucosinolate used in the present invention is plant extracts, safe and healthy；And zeatin and sucrose are in daily life
It has been widely used and cheap, so cost is cheap and safe and effective.
Claimed technical scheme is deeply ground in influence of the various technical factors to grafting wound healing
Drawn on the basis of studying carefully, reason is as follows：
First, influence of the different ratio preparation to grafting wound healing
Processing mode is the combination of glucosinolate, zeatin and sucrose various concentrations.The concentration of glucosinolate chooses 20 μ respectively
M, 50 μM and 100 μM, zeatin concentration is 1 μM, 3 μM and 5 μM, sets blank control.Experimental result is：20 μM
When glucosinolate and 3 μM of zeatin merging treatment, there is certain facilitation, but when zeatin concentration increases to 5 μM, promote
Enter DeGrain；50 μM of glucosinolate and 3 μM of zeatin are jointly processed by being remarkably improved the healing speed of grafting wound
Degree, and it lifts effect when effect will be significantly greater than the two independent processing；100 μM of glucosinolate and 5 μM of zeatin
When being jointly processed by, the healing rate of grafting wound is slower, and the callus of wound becomes irregular, directly affects the later stage
Healing, so filter out glucosinolate and 3 μM of zeatin that optimal concentration for the treatment of is 50 μM.On this basis, sugarcane is designed
Sugared concentration is 1wt%, 3wt% and 5wt%, be jointly processed by respectively with 50 μM of glucosinolate and 3 μM of zeatin graft stock and
The wound of scion, the results showed that, 3 wt% concentration can dramatically increase the promotion of glucosinolate and zeatin to wound healing
Effect, 5 wt% sucrose concentration make it that the wound dehydration of scion and stock is obvious, are unfavorable for wound healing.Tested with this and be
Foundation, filters out glucosinolate, 3 μM of zeatin and 3wt% sucrose that optimal preparation is 50 μM, ethanol and Tween 80
Dosage does not have too big influence then to wound healing.
2nd, using influence of the grafting seedling age to grafting wound healing during preparation
It is determined that in preparation after the optium concentration of main component, using the tomato scion and stock of different seedling ages as material, including three
The individual stage（Two leaves wholeheartedly, three leaves wholeheartedly with four leaves wholeheartedly）, after healing formulation is handled, it is found that the healing of four leaves wholeheartedly young plant
It is fastest.
3rd, using influence of the mode of preparation to grafting wound healing
Change preparation method of application grafting wound healing can also be had an impact, test three kinds of mode of healing include smear,
Immersion and sprinkling.As a result show, the effect for soaking 1h is best.
The beneficial effects of the present invention are：
The present invention, as the main component of preparation, can promote grafting wound healing using glucosinolate, zeatin and sucrose, improve wound
Mouth healing rate, shortens healing time.
Embodiment
For the further open rather than limitation present invention, below in conjunction with example, the present invention is described in further detail.
Embodiment 1
One kind improves tomato anvil fringe grafting wound healing preparations, and its component is as follows：50 μM, sucrose 3wt% of glucosinolate, corn
3 μM of element, volumetric concentration are 95% mL of ethanol 50, and volumetric concentration is 98% mL of Tween 80 15, adds water to be settled to 1L.
Compound method：Each component is weighed according to the above ratio, after zeatin is first dissolved in ethanol, adds Tween 80, mixing
Uniformly, solution 1 is obtained；The solution 1 is well mixed with glucosinolate again, sucrose is added, is 1L with water constant volume.
Using stock " strength " and " being full of again " kind as experiment material, four leaves wholeheartedly when soak stock and scion wound,
Time is 1h.
Wound healing rate is 88%, healing time 65h.
Embodiment 2
One kind improves tomato anvil fringe grafting wound healing preparations, and its component is as follows：20 μM, sucrose 3wt% of glucosinolate, corn
3 μM of element, volumetric concentration are 95% mL of ethanol 50, and volumetric concentration is 98% mL of Tween 80 15, adds water to be settled to 1L.
Compound method：Each component is weighed according to the above ratio, after zeatin is first dissolved in ethanol, adds Tween 80, mixing
Uniformly, solution 1 is obtained；The solution 1 is well mixed with glucosinolate again, sucrose is added, is 1L with water constant volume.
Using stock " strength " and " being full of again " kind as experiment material, four leaves wholeheartedly when soak stock and scion wound,
Time is 1h.
Wound healing rate is 83%, healing time 68h.
Embodiment 3
One kind improves tomato anvil fringe grafting wound healing preparations, and its component is as follows：100 μM, sucrose 5wt% of glucosinolate, corn
5 μM of element, volumetric concentration are 95% mL of ethanol 50, and volumetric concentration is 98% mL of Tween 80 15, adds water to be settled to 1L.
Compound method：Each component is weighed according to the above ratio, after zeatin is first dissolved in ethanol, adds Tween 80, mixing
Uniformly, solution 1 is obtained；The solution 1 is well mixed with glucosinolate again, sucrose is added, is 1L with water constant volume.
Using stock " strength " and " being full of again " kind as experiment material, four leaves wholeheartedly when soak stock and scion wound,
Time is 1h.
Wound healing rate is 83%, healing time 70h.
Embodiment 4
One kind improves tomato anvil fringe grafting wound healing preparations, and its component is as follows：50 μM, sucrose 3wt% of glucosinolate, corn
3 μM of element, volumetric concentration are 95% mL of ethanol 50, and volumetric concentration is 98% mL of Tween 80 15, adds water to be settled to 1L.
Compound method：Each component is weighed according to the above ratio, after zeatin is first dissolved in ethanol, adds Tween 80, mixing
Uniformly, solution 1 is obtained；The solution 1 is well mixed with glucosinolate again, sucrose is added, is 1L with water constant volume.
Using stock " god of unusual strength " and " rich Europe " kind as experiment material, four leaves wholeheartedly when soak stock and scion wound
Place, time 1h.
Wound healing rate is 89%, healing time 66h.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, it should all belong to the covering scope of the present invention.
Claims (5)
- A kind of 1. preparation for grafting wound healing, it is characterised in that：Said preparation in terms of 1L, consisting of：Mustard oil 2 μM ~ 100 μM of glycosides, 1 μM ~ 10 μM, sucrose 1wt% ~ 10wt% of zeatin, volumetric concentration be 95% ethanol 50mL ~ 125mL, volume Concentration is 98% Tween 80 11.1mL ~ 67.2mL, and surplus is water.
- 2. the preparation according to claim 1 for grafting wound healing, it is characterised in that：Said preparation in terms of 1L, Consisting of：50 μM of glucosinolate, 3 μM, sucrose 3wt% of zeatin, volumetric concentration are 95% ethanol 50mL, and volumetric concentration is 98% Tween 80 15mL, surplus are water.
- 3. a kind of method for preparing the preparation as claimed in claim 1 or 2 for grafting wound healing, its feature exist In：After first zeatin being dissolved in the ethanol that volumetric concentration is 95%, the Tween 80 that volumetric concentration is 98% is added, is well mixed, Solution 1 is made；Solution 1 and glucosinolate are mixed together uniformly again, sucrose is then added, stirs, be 1L with water constant volume.
- 4. a kind of application method of the preparation as claimed in claim 1 or 2 for grafting wound healing, its feature exist In：Preparation is applied to wound, either grafting wound is soaked in preparation or preparation is sprayed on grafting wound.
- 5. the application method of the preparation according to claim 4 for grafting wound healing, it is characterised in that：It will transfer Connect wound to be soaked in preparation, soak time 1h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711235624.1A CN107668051A (en) | 2017-11-30 | 2017-11-30 | A kind of preparation for promoting grafting wound healing |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711235624.1A CN107668051A (en) | 2017-11-30 | 2017-11-30 | A kind of preparation for promoting grafting wound healing |
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| CN107668051A true CN107668051A (en) | 2018-02-09 |
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| CN201711235624.1A Pending CN107668051A (en) | 2017-11-30 | 2017-11-30 | A kind of preparation for promoting grafting wound healing |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109937726A (en) * | 2019-02-19 | 2019-06-28 | 温州科技职业学院 | A method of the grafting of tomato casing is carried out using stem section |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524260A (en) * | 2011-12-25 | 2012-07-04 | 浙江大学 | Preparation for increasing content of glucosinolate in Chinese kale sprouts |
| CN104886157A (en) * | 2015-05-08 | 2015-09-09 | 长阳勤劳农夫农产品有限公司 | Grafting treatment agent and application thereof on grafting stock for solanaceae crops |
-
2017
- 2017-11-30 CN CN201711235624.1A patent/CN107668051A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102524260A (en) * | 2011-12-25 | 2012-07-04 | 浙江大学 | Preparation for increasing content of glucosinolate in Chinese kale sprouts |
| CN104886157A (en) * | 2015-05-08 | 2015-09-09 | 长阳勤劳农夫农产品有限公司 | Grafting treatment agent and application thereof on grafting stock for solanaceae crops |
Non-Patent Citations (1)
| Title |
|---|
| 苗慧莹等: "芥子油苷代谢调控和葡萄糖信号转导研究进展", 《食品安全质量检测学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109937726A (en) * | 2019-02-19 | 2019-06-28 | 温州科技职业学院 | A method of the grafting of tomato casing is carried out using stem section |
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Application publication date: 20180209 |