CN102365962A - Healing promotion agent for grafting, its preparation method and its application - Google Patents
Healing promotion agent for grafting, its preparation method and its application Download PDFInfo
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- CN102365962A CN102365962A CN2010105237091A CN201010523709A CN102365962A CN 102365962 A CN102365962 A CN 102365962A CN 2010105237091 A CN2010105237091 A CN 2010105237091A CN 201010523709 A CN201010523709 A CN 201010523709A CN 102365962 A CN102365962 A CN 102365962A
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Abstract
The invention discloses a healing promotion agent for grafting which is capable of promoting the survival rate of grape hard-twig grafting. The healing promotion agent for grafting comprises 0.03-0.05% of phenyl thiadiazole urea, 4-6% of dimethyl formamide, 40-60% of ethanol with 95% of concentration and 33.95-55.97% of water. The invention discloses a method for preparing the healing promotion agent for grafting and a method for using the healing promotion agent for grafting. The healing promotion agent for grafting of the invention is capable of substantially raising the survival rate of grape grafting.
Description
Technical field
The invention belongs to the plant cultivation field, be specifically related to short more agent, its preparation method and the application of a kind of grafting.
Background technology
Because the harm of grape phylloxera; Bring into use stock in the wine-growing, the research of grape rootstock and the application of graft technology make the stable development of grape industry; Adopt the good grape rootstock of strong stress resistance to carry out grafting cultivation, become the important behave of development grape industry.Stiff wood grafting is the major technique of grape grafting breeding; And the affinity of stock and scion is the principal element of decision grafting success or not, and some stock variety has very strong resistance, but poor with the main breed compatibility; Very difficult quilt directly utilizes; Therefore stiff wood grafting affinity is poor, and survival rate is lower, and grafting efficiency is not high.
In recent years, along with deepening continuously of research, growth regulator has obtained extensive use in agricultural production.Growth regulator can improve graft survival rate, and the germination and growth that promotion is buddered is at the existing report of seeds such as walnut, jujube, citrus, apricot.And research is less aspect grape.Only the Chu Yan outstanding person of Hebei province's Zhuolu County State Forestry Administration, P.R. China when kind is changed in the stump grafting, utilized the ABT root-inducing powder to dip scion to 8 years living longan cultivars (non-stock variety, non-exsomatizing) in 1998, and the cleft grafting survival rate reaches (Chu Yanjie, 1998) more than 85%.In addition, the at present domestic report of not seeing the short agent of healing of the stripped stiff wood grafting of relevant grape.
Summary of the invention
What the present invention will solve is the low problem of vine hard branch graft survival rate in the prior art, provides a kind of grafting short more agent, its preparation method and application.
More agent is urged in grafting provided by the invention, contains the component of following weight percentage:
Phenyl thiadiazolyl group urea: 0.03～0.05%
Dimethyl formamide: 4～6%
95% ethanol: 40～60%
Water: 33.95～55.97%.
Preferred percentage by weight is:
Phenyl thiadiazolyl group urea: 0.04%
Dimethyl formamide: 5%
95% ethanol: 50%
Water: 44.96%.
The present invention also provides the above-mentioned short more preparation method of agent, comprises the steps:
Take by weighing the phenyl thiadiazolyl group urea (TDZ) of formula rate, be dissolved in the dimethyl formamide of formula rate, add 95% ethanol of formula rate, use the water constant volume of formula rate at last.
The short more application of agent in the grape grafting of the grafting of the present invention's preparation; The preferably application in the vine hard branch grafting; Specifically be with the scion base portion 3～5cm that cuts and stock top 3～5cm, immerse the 10～15sec in the more agent that urgees of 5～6 times of dilutions, carry out grafting then.
Phenyl thiadiazolyl group urea (TDZ) is a kind of new plant growth regulator, and very strong cytokine activity is arranged, and can promote callus growth, has the specific function of growth hormone and basic element of cell division double action.When grape growth was carried out grafting season, the cambial cell of stock and scion was dipped the short more agent of the present invention all in continuous division in the wound surface of cutting, can stimulate cambial cell to quicken division, forms callus.In addition, the concentration of wound growth hormone is increased, promote cell division and growth, form more callus, help the anvil fringe and progressively form a community, improve graft compatibility.
Compare with the clear water contrast, short more agent of the present invention can significantly improve germination rate, interface healing rate, root callus rate, germination-healing rate, germination-callus rate, germination-healing-callus rate.Common stiff wood grafting survival rate is lower in the present domestic actual production, carries out stiff wood grafting and use this short more agent, and the interface healing rate reaches as high as 100%, and survival rate can reach more than 85%.
Embodiment
Following examples are used to explain the present invention, but are not used for limiting scope of the present invention.
Embodiment 1
Take by weighing phenyl thiadiazolyl group urea (TDZ) 30mg and put into beaker, other takes by weighing DMF 4g and puts into beaker, stirs fully dissolving with glass bar, adds 95% ethanol 40g subsequently, adds entry 55.97g after stirring and processes short more agent.
Embodiment 2
Take by weighing phenyl thiadiazolyl group urea (TDZ) 40mg and put into beaker, other takes by weighing DMF 6g and puts into beaker, stirs fully dissolving with glass bar, adds 95% ethanol 60g subsequently, adds entry 33.96g after stirring and processes short more agent.
Embodiment 3
Take by weighing phenyl thiadiazolyl group urea (TDZ) 50mg and put into beaker, other takes by weighing DMF 5g and puts into beaker, stirs fully dissolving with glass bar, adds 95% ethanol 50g subsequently, adds entry 44.95g after stirring and processes short more agent.
Embodiment 4
Take by weighing phenyl thiadiazolyl group urea (TDZ) 40mg and put into beaker, other takes by weighing DMF 5g and puts into beaker, stirs fully dissolving with glass bar, adds 95% ethanol 50g subsequently, adds entry 44.96g after stirring and processes short more agent.
Embodiment 5101-14 is a stock grafting cabernet sauvignon grape kind
1. plant the bar collection
The grape fallen leaves back combination winter cuts and carries out, and gathers from pure, the healthy and strong Cabernet Sauvignon of kind, 101-14 plant.Per 50 kind bar a bundles are tied up neatly, perform mark.Going into ditch buries.The previous day is taken out kind of a bar in grafting, soaks 12～24h with clear water, makes it fully suction moisture.
2. stiff wood grafting
Carry out in the greenhouse.Select 1～2 full bud at scion Cabernet Sauvignon kind branch, in the above 2cm of top bud and 3～4cm place intercepting below the bud of bottom.Both sides become the long bevel of 2～3cm respectively to central cutting under bud, and bevel must be level and smooth, is wedge shape.Stock 101-14 kind bar is cut into the stem section of 20～25cm, clip straight snips in the requirement, following clip tiltedly is cut into the shape of a hoof, and will get rid of the eye on the stock, influences graft survival rate to prevent stock bud from sprouting.Scion base portion and stock top 3cm are immersed the short more agent 15sec of embodiment 4 preparations that are diluted to 5 times.Contrast is immersed 15sec in the clear water with scion base portion and stock top 3cm.Choose rugosity consistent scion and stock, in the partial application of stock central longitudinal, notch depth is consistent with scion bevel length, inserts the scion of cutting along otch, and scion is alignd with the stock cambium.With the wide plastic strip of 1cm grafting mouth and scion are partly tied up sternly then.At last, the stock water chestnut that grafting is accomplished is faced together, 10 1 bundle, dipped in the ABT2 of concentration 1g/kg rooting powder solution middling speed.
3. go to bed and urge root
In plastic tunnel or greenhouse, lay hot line hotbed double-deckly up and down.Lay bottom ground hot line earlier, the wet sand that line upper berth 4～5cm is thick.The 2nd layer (upper strata) hot line exceed lower floor ground hot line 20cm, at each side fixing 1 plank of hotbed, laterally lay the ground hot line, lay on sign indicating number kind bar limit, limit, width is decided according to 1 bundle of kind bar rugosity.The interface of every bundle of kind bar is between two adjacent lines of ground, upper strata hot line.Pour into fine sand and the deposition of pouring water between bundle, outside eye reveals.The temperature on upper strata is controlled at 28～29 ℃, and the temperature of lower floor is controlled at 24～25 ℃.Note the humidity in control greenhouse temperature and seedbed.Carry out the affine performance investigation of grafting nursery stock about 20d, following project is added up: rudiment, interface healing, root callus, germination-healing, germination-callus, germination-healing-callus.After having added up, stop to heat.Take exercise after 3～5 days, can insert in the greenhouse nutritive cube.Wherein, rudiment finger fringe eye is sprouted; Interface healing finger fringe and the healing of stock grafting mouth; The root callus is meant that the stock base section dissolves callus; Germination-healing is meant the sprouting of scion bud, and scion and the healing of stock grafting mouth; Germination-callus finger fringe eye is sprouted, and the stock base section dissolves callus; Germination-healing-callus is meant the sprouting of scion eye, and scion and the healing of stock grafting mouth, and the stock base section dissolves callus.
4. cultivate in the greenhouse
In the greenhouse, do the seedbed, wide 1.0～1.5m, length is looked warm canopy width and is decided, dark 30cm.With 2 parts in thick river sand, show to pack into after 3 parts in soil is fully mixed thoroughly in the nutritious bag in the field.Native face and sack after dress is real maintain an equal level.With the nutritious bag marshalling, to pour water earlier, transplant a cutting the back.Note the humidity in control greenhouse temperature and seedbed, the sprouting that sprouts on the stock of in time erasing is strengthened the water and fertilizer management and the extermination of disease and insect pest, promotes seedling growth.
5. transplant in the field
When treating nursery stock length to the 15cm left and right sides, begin progressively ventilation and penetrating light, the fertile refining of control water control seedling is transplanted to field field planting ditch then.Seeding row spacing 0.5～1.0 * 2.5～4.0m.
Table 1 clear water is handled and short more agent is handled Cabernet Sauvignon/101-14 effect relatively
Can find out that from table 1 Cabernet Sauvignon is scion, 101-14 is a stock, and after short more agent processing, germination rate, interface healing rate, root callus rate, germination-healing rate, germination-callus rate, germination-healing-callus rate all are higher than the clear water contrast.The result shows, handles the graft survival rate that can significantly improve grape with short more agent.
Embodiment 6 is a stock grafting summer Black Grape kind with 5BB
1. plant the bar collection
Grape fallen leaves back combines the winter to cut to carry out, and black and 5BB plant is gathered from pure, the healthy and strong summer of kind.Per 50 kind bar a bundles are tied up neatly, perform mark.Going into ditch buries.The previous day is taken out kind of a bar in grafting, soaks 12～24h with clear water, makes it fully suction moisture.
2. stiff wood grafting
Carry out in the greenhouse.Select 1～2 full bud at black kind branch of scion summer, in the above 2cm of top bud and 3～4cm place intercepting below the bud of bottom.Both sides become the long bevel of 2～3cm respectively to central cutting under bud, and bevel must be level and smooth, is wedge shape.Stock 5BB kind bar is cut into the stem section of 20～25cm, clip straight snips in the requirement, following clip tiltedly is cut into the shape of a hoof, and will get rid of the eye on the stock, influences graft survival rate to prevent stock bud from sprouting.Scion base portion and stock top 5cm are immersed the short more agent 10sec of embodiment 3 preparations that are diluted to 6 times.Contrast is immersed 10sec in the clear water with scion base portion and stock top 5cm.Choose rugosity consistent scion and stock, in the partial application of stock central longitudinal, notch depth is consistent with scion bevel length, inserts the scion of cutting along otch, and scion is alignd with the stock cambium.With the wide plastic strip of 1cm grafting mouth and scion are partly tied up sternly then.At last, the stock water chestnut that grafting is accomplished is faced together, 10 1 bundle, dipped in the ABT2 of concentration 1g/kg rooting powder solution middling speed.
3. go to bed and urge root
In plastic tunnel or greenhouse, lay hot line hotbed double-deckly up and down.Lay bottom ground hot line earlier, the wet sand that line upper berth 4～5cm is thick.The 2nd layer (upper strata) hot line exceed lower floor ground hot line 20cm, at each side fixing 1 plank of hotbed, laterally lay the ground hot line, lay on sign indicating number kind bar limit, limit, width is decided according to 1 bundle of kind bar rugosity.The interface of every bundle of kind bar is between two adjacent lines of ground, upper strata hot line.Pour into fine sand and the deposition of pouring water between bundle, outside eye reveals.The temperature on upper strata is controlled at 28～29 ℃, and the temperature of lower floor is controlled at 24～25 ℃.Note the humidity in control greenhouse temperature and seedbed.Carry out the affine performance investigation of grafting nursery stock about 20d, following project is added up: rudiment, interface healing, root callus, germination-healing, germination-callus, germination-healing-callus.After having added up, stop to heat.Take exercise after 3～5 days, can insert in the greenhouse nutritive cube.
4. cultivate in the greenhouse
In the greenhouse, do the seedbed, wide 1.0～1.5m, length is looked warm canopy width and is decided, dark 30cm.With 2 parts in thick river sand, show to pack into after 3 parts in soil is fully mixed thoroughly in the nutritious bag in the field.Native face and sack after dress is real maintain an equal level.With the nutritious bag marshalling, to pour water earlier, transplant a cutting the back.Note the humidity in control greenhouse temperature and seedbed, the sprouting that sprouts on the stock of in time erasing is strengthened the water and fertilizer management and the extermination of disease and insect pest, promotes seedling growth.
5. transplant in the field
When treating nursery stock length to the 15cm left and right sides, begin progressively ventilation and penetrating light, the fertile refining of control water control seedling is transplanted to field field planting ditch then.Seeding row spacing 0.5～1.0 * 2.5～4.0m.。
The summer is handled in processing of table 2 clear water and short more agent, and black/5BB effect compares
Can find out that from table 2 summer is black to be scion, 5BB is a stock, and after handling with the short agent of healing, germination rate, interface healing rate, root callus rate, germination-healing rate, germination-callus rate, germination-healing-callus rate all are higher than clear water and contrast.The result shows, handles the graft survival rate that can significantly improve grape with short more agent.
According to the present invention, also tested the short more agent of other embodiment preparations, the result shows that all the short more agent of preparation all can significantly improve the survival rate of vine hard branch grafting.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from know-why of the present invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (5)
1. more agent is urged in a grafting, contains the component of following weight percentage:
Phenyl thiadiazolyl group urea: 0.03～0.05%
Dimethyl formamide: 4～6%
95% ethanol: 40～60%
Water: 33.95～55.97%.
2. more agent is urged in grafting according to claim 1, contains the component of following weight percentage:
Phenyl thiadiazolyl group urea: 0.04%
Dimethyl formamide: 5%
95% ethanol: 50%
Water: 44.96%.
3. the short more agent of preparation claim 1 or 2 described graftings comprises the steps:
Take by weighing the phenyl thiadiazolyl group urea of formula rate, be dissolved in the dimethyl formamide of formula rate, add 95% ethanol of formula rate, use the water constant volume of formula rate at last.
4. the short more application of agent in the vine hard branch grafting of claim 1 or 2 described graftings.
5. use the short more method of agent of claim 1 or 2 described graftings, it comprises the scion base portion 3～5cm that cuts and stock top 3～5cm, immerses 10～15sec in the short more agent of the grafting of diluting 5～6 times, carries out grafting then.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105237091A CN102365962A (en) | 2010-10-28 | 2010-10-28 | Healing promotion agent for grafting, its preparation method and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105237091A CN102365962A (en) | 2010-10-28 | 2010-10-28 | Healing promotion agent for grafting, its preparation method and its application |
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| CN102365962A true CN102365962A (en) | 2012-03-07 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104886157A (en) * | 2015-05-08 | 2015-09-09 | 长阳勤劳农夫农产品有限公司 | Grafting treatment agent and application thereof on grafting stock for solanaceae crops |
| CN106508964A (en) * | 2016-10-09 | 2017-03-22 | 湖南省西瓜甜瓜研究所 | Healing agent for improving mechanized grafting survival rate of watermelon |
| CN110896742A (en) * | 2019-11-20 | 2020-03-24 | 中徽生态环境有限公司 | Red maple grafting seedling method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1036124A (en) * | 1987-07-16 | 1989-10-11 | 奇诺英药物化学工厂有限公司 | Plant protection composition |
| CN101103727A (en) * | 2007-08-17 | 2008-01-16 | 咸阳德丰有限责任公司 | Method for preparing thidiazuron as active substance used for plant quality-promoting and yield-increasing product and its application |
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2010
- 2010-10-28 CN CN2010105237091A patent/CN102365962A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1036124A (en) * | 1987-07-16 | 1989-10-11 | 奇诺英药物化学工厂有限公司 | Plant protection composition |
| CN101103727A (en) * | 2007-08-17 | 2008-01-16 | 咸阳德丰有限责任公司 | Method for preparing thidiazuron as active substance used for plant quality-promoting and yield-increasing product and its application |
Non-Patent Citations (1)
| Title |
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| 徐志达等: "益果灵在果树上的应用效果与机理探讨", 《西北园艺》, no. 04, 10 April 2004 (2004-04-10) * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104886157A (en) * | 2015-05-08 | 2015-09-09 | 长阳勤劳农夫农产品有限公司 | Grafting treatment agent and application thereof on grafting stock for solanaceae crops |
| CN104886157B (en) * | 2015-05-08 | 2017-10-10 | 湖北勤劳农夫生态农业股份有限公司 | A kind of grafting inorganic agent and the application on solanaceous crops graft stock |
| CN106508964A (en) * | 2016-10-09 | 2017-03-22 | 湖南省西瓜甜瓜研究所 | Healing agent for improving mechanized grafting survival rate of watermelon |
| CN106508964B (en) * | 2016-10-09 | 2019-03-15 | 湖南省农业生物技术研究所 | A kind of consolidant and its application method improving watermelon mechanization grafting survival rate |
| CN110896742A (en) * | 2019-11-20 | 2020-03-24 | 中徽生态环境有限公司 | Red maple grafting seedling method |
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Application publication date: 20120307 |