CN101870604B - Composite oligosaccharide plant growth stimulator and preparation method thereof - Google Patents
Composite oligosaccharide plant growth stimulator and preparation method thereof Download PDFInfo
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- 239000003324 growth hormone secretagogue Substances 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a composite oligosaccharide plant growth stimulator and a preparation method thereof, wherein the composite oligosaccharide plant growth stimulator comprises the following components and contents (calculated by weight percent): 1-5% of oligomerization chitosan, 1-5% of fructo oligosaccharide, 1-2% of oligomerization glucan, 10% of beer yeast hydrolysate, 0.5-1% of trace element, 10% of KH2PO4 and the balance of chicken manure water extract, which add up to 100%. The preparation method comprises the steps of: (1) preparing beer yeast hydrolysate; and (2) mixing the beer yeast hydrolysate obtained in the step (1) with oligomerization chitosan, fructo oligosaccharide, oligomerization glucan, trace element, KH2PO4 and chicken manure water extract according to the ratio, and obtaining the composite oligosaccharide plant growth stimulator. The invention is non-toxic, harmless and environmental friendly, can stimulate the growth and prematurity of plants, improve theyield, can improve the stress resistance of the plants and the quality of medicinal materials, and has wide application scope.
Description
Technical field
The present invention relates to a kind of plant growth promoter and preparation method thereof, particularly a kind of plant growth promoter that contains composite oligosaccharides such as chitosan oligomer, fructo-oligosaccharide (FOS) and oligomeric glucan, brewer's yeast hydrolysate and preparation method thereof.
Background technology
As everyone knows, quick growth along with high speed development and the population of production technology, people require the hope of soil volume increase urgent day by day, the use of a large amount of chemical fertilizer and agricultural chemicals, harm to soil and crops is very serious, and the environment that people depend on for existence polluted, and harm people's health, modern scientific research shows, the growth of plant not only needs common nitrogen, phosphorus, potash fertilizer also needs necessary trace element, compound amino acid and a certain amount of plant growth promoter, existing various promotion plant growing agents all can not maximizedly reach the optimum efficiency that promotes the plant growth, use the face of plant variety less, the effect of increasing production instability.
Summary of the invention
Technical problem to be solved by this invention provide a kind of non-pollution safety, scope of application green plants growth promoter and preparation method thereof widely, its effect is by strengthening, regulate and control the activity of phytoenzyme, the activation plant cell, the morphogenesis of inducing plant adventive root or indefinite bud, promote formation and the growth of root system at short notice, regulate the plant metabolism action intensity, reach and improve plant growth rate, crop yield, increase nutrient content of plant and the purpose that improves disease-resistant performance.
The present invention solves the technical scheme that its technical problem takes: a kind of composite oligosaccharide plant growth stimulator, described composite oligosaccharide plant growth stimulator comprise following component and content (content is in weight %):
Chitosan oligomer (mean molecule quantity 1000---5000) 1-5%,
Fructo-oligosaccharide (FOS) (molecular weight 2000---3000) 1-5%, oligomeric glucan (2000-3000) 1-2%,
Brewer's yeast hydrolysate 10%, the trace element (Mo, B, mishmetal, Fe) 0.5-1%,
KH
2P0
410%, all the other add to 100% for the chicken manure water extract.
Wherein chitosan oligomer is by employing cellulase hydrolysis and the preparation of hydrogen peroxide biodegrading process, separates with milipore filter, and atomized drying forms, and molecular weight detection adopts gel chromatography, and molecular weight ranges is 1000---5000.Fructo-oligosaccharide (FOS) is that Radix Arctii extracts preparation.Oligomeric glucan is that beta glucan makes through the β enzymatic hydrolysis.
The preparation method of composite oligosaccharide plant growth stimulator is as follows:
(1) preparation of brewer's yeast hydrolysate:
1. brewer's yeast is added in 15 times of water (weight ratio); 2. in the aqueous solution of brewer's yeast, add 0.5% dextranase, 1% papain, 1% cellulase; 3. adding the acetic acid adjust pH is 5.5; 4. under 50 ℃ temperature, reacted 4 hours; 5. the unreacted brewer's yeast of elimination; 6. concentration of reaction solution, vacuum drying obtains brown powder, wherein contains amino acid 70-80%, oligosaccharides 20-30%.
(2) with the brewer's yeast hydrolysate that obtains in the step (1) and chitosan oligomer, fructo-oligosaccharide (FOS), oligomeric glucan, trace element (Mo, B, mishmetal, Fe), KH
2PO
4, the chicken manure water extract mixes according to above-described ratio and namely gets composite oligosaccharide plant growth stimulator.
The proportioning mode of optimum of the present invention further is:
Chitosan oligomer (mean molecule quantity 1000---5000) 2%,
Fructo-oligosaccharide (FOS) (molecular weight 2000---3000) 2%, oligomeric glucan (2000-3000) 1%,
Brewer's yeast hydrolysate 10%, the trace element (Mo, B, mishmetal, Fe) 0.5%,
KH
2PO
410%, all the other add to 100% for the chicken manure water extract.
The preparation method of composite oligosaccharide plant growth stimulator of the present invention is as follows:
Sieve below above-mentioned each component solid material is crushed to 100 orders, fully mix in the solid batch mixer according to optimization formula, sealing gets final product in the plastic sack of packing into.
Using method: composite oligosaccharide plant growth stimulator 10 grams are dissolved in 20 kg of water, can spray with root plant with this solution and irritate.
Composite oligosaccharide plant growth stimulator of the present invention has the following advantages:
1, all nontoxic, environmental protection of all components, harmless, be applicable to that the raw material planting of pollution-free food is used, be a kind of safe green plants growth promoter.
2, composite oligosaccharide plant growth stimulator of the present invention contains nutriments such as multiple oligosaccharide, several amino acids, various trace elements and the nitrogen that promotes plant to grow, phosphorus, potassium.Can promote the growth of root system of plant and cauline leaf.Should short long agent can improve the plant water imbibition, promote photosynthesis, can increase substantially crop yield, and generally can increase production 10-30%.Improve nutrient component in the plant, increase mouthfeel and the outward appearance of fruit.
3, this promoter can be improved the anti-adversity of plant, for example: improve plant drought, cold-resistant, disease-resistant, resistant to lodging performance.This product can be secreted a large amount of protective plant protecting agents by stimulating plant, improves stress resistance of plant, comprises the bacteriostasis property that improves plant.Therefore, this product can to a certain degree play control iron rust disease, prevent and treat powdery mildew etc.
4, this product can improve the active constituent content in the Chinese medicinal plant, improves the traditional Chinese medicine quality.As: improve the content of astragalus polyose content, raising tanshinone IIA, the content of raising glycyrrhizic acid etc., main because the oligosaccharides in this product can accumulate secondary metabolites generation protective plant protecting agent and polysaccharide by stimulating plant.
5, this plant promoting agent can promote jarovize, and for example corn, paddy rice, leafy vegetable etc. generally can shift to an earlier date 7-10 days ripe results.
6, this plant growth promoter has wide range of applications, applicable to various leafy vegetables, melon and fruit, rhizome vegetables, plant medicine material, flowers, wheat, paddy rice, soybean, tealeaves, tree cuttage etc.
Embodiment
Experimental results show that chitosan oligomer, fructo-oligosaccharide (FOS) and oligomeric glucan etc. have the effect that promotes plant growth and raising plant stress-resistance performance, by strengthening, regulate and control the activity of phytoenzyme, the activation plant cell, promote the synthetic of large biological molecule, the morphogenesis of inducing plant adventive root or indefinite bud, promote formation and the growth of root system at short notice, regulate the plant metabolism action intensity, reach and improve plant growth rate, crop yield, increase nutrient content of plant and the purpose that improves disease-resistant performance.Composite oligosaccharide is stronger than the effect of single oligosaccharide, and brewer's yeast produces oligomeric glucan, oligopeptides and several amino acids by multiple enzymolysis, and the plant growth is had facilitation.The plant growth promoter of forming by composite oligosaccharide, brewer's yeast hydrolysate and various trace elements does not contain artificial synthetic hormone, environment is not polluted, and not producing residually in crops, use safety, is a kind of novel green plant growth promoter.Below be illustrated with embodiment.
Embodiment 1 composite oligosaccharide plant growth stimulator is to romaine lettuce growth and yield effect
Composite oligosaccharide plant growth stimulator is added the ratio that 20 kg of water and 10 grams add 30 kg of water according to 10 grams be mixed with solution, carry out control experiment with clear water respectively, the romaine lettuce seed is soaked soaked in three hours, sowing at the beginning of 9 months.20 strains of taking a sample respectively in the 4th day, the 8th day, the 11st day investigation plant maximum root, the horizontal stem of maximum root, the test of taking root.
The aqueous solution (containing the clear water contrast) for preparing above-mentioned promoter is sprayed processing to romaine lettuce, spray behind 5 leaves when seedling is long, sprayed once in per 7 days, the whole growth cycle handles 3 times with promoter.
The romaine lettuce seed soaks the back germination rate through accelerator solution and obviously improves, and seedling is irritated length and the root diameter of executing the back root through the promoter root obviously to be increased, and sprays this plant growth promoter in the vegetative period of romaine lettuce, and plant strain growth is accelerated, blade increases.Through after spraying, the chlorophyll of romaine lettuce, vitamin C, content of reducing sugar all increase, and per mu yield increases by 8%.After the romaine lettuce seed soaked composite oligosaccharide plant growth stimulator, the growing state of root saw Table 1.
Table 1: after the romaine lettuce seed soaks composite oligosaccharide plant growth stimulator, the growing state of root
Spraying back 5 days of processing, random sampling 10 strains investigation biological character the results are shown in Table 2 at every turn.
Table 2: biological character application form
After spraying through three times as can be seen from Table 2,0.03% concentration group plant height, the number of blade, maximum leaf are grown, leaf Da Ye is wide all apparently higher than the clear water group, illustrate that composite oligosaccharide plant growth stimulator can promote the romaine lettuce growth.
Table 3: composite oligosaccharide plant growth stimulator is handled back romaine lettuce output statistics table
Unit: Kg
After handling through composite oligosaccharide plant growth stimulator as can be seen from Table 3, all increase than control group per mu yield, wherein 0.03% group of volume increase is the most remarkable.
Embodiment 2 composite oligosaccharide plant growth stimulators are to romaine lettuce growth and yield effect
The test cucumber is adopted and extensively grinds No. 3
Composite oligosaccharide plant growth stimulator is added the ratio that 20 kg of water and 10 grams add 30 kg of water according to 10 grams be mixed with solution, carry out control experiment with clear water respectively, cucumber seeds is soaked soaked in three hours, sowing at the beginning of 9 months.Got sheep 20 strains investigation plant maximum root, the horizontal stem of maximum root in the 3rd day, the 6th day, the 9th day respectively, the test of taking root.
Moonrise at 2007 9 sows, and promotes growth test, establishes three groups, three times and repeats, totally 18 districts, 20 square metres of each residential quarter areas.Experimental result is as follows:
Table 4: after composite oligosaccharide plant growth stimulator soaks seed, to the thick influence of root
As can be seen from Table 4, behind composite oligosaccharide plant growth stimulator immersion seed, the root of cucumber slightly all is higher than the clear water group.
Table 5: after composite oligosaccharide plant growth stimulator soaks seed, the influence long to root
As can be seen from Table 5, behind composite oligosaccharide plant growth stimulator immersion seed, the long clear water group that all is higher than of the root of cucumber.Above presentation of results composite oligosaccharide plant growth stimulator has obvious facilitation to the root growth of cucumber.
Table 6: composite oligosaccharide plant growth stimulator is handled the influence of back cucumber aerial growth
As can be seen from Table 6, after the process composite oligosaccharide plant growth stimulator sprays processing, the plant height of cucumber, the long and wide control group that all is higher than of leaf Da Ye of climing thick, maximum leaf, it is best that wherein 0.03% concentration group is handled thick long-acting fruit, illustrates that composite oligosaccharide plant growth stimulator has obvious facilitation to the cucumber aerial growth.
Table 7: composite oligosaccharide plant growth stimulator is handled back cucumber yield statistical form
Unit: Kg
Cucumber per mu yield and eurymeric melon rate all are significantly increased after handling through composite oligosaccharide plant growth stimulator as can be seen from Table 7.
Claims (3)
1. a composite oligosaccharide plant growth stimulator is characterized in that, comprises following component and content, and content is in weight %:
Range of molecular weight distributions is at the chitosan oligomer 1-5% of 1000-5000
Range of molecular weight distributions is at the fructo-oligosaccharide (FOS) 1-5% of 2000-3000
Range of molecular weight distributions is at the oligomeric glucan 1-2% of 2000-3000
Brewer's yeast hydrolysate 10%
Trace element 0.5-1%
KH
2PO
410％
All the other are the chicken manure water extract, add to 100%;
Described trace element is Mo, B, mishmetal, Fe;
Described brewer's yeast hydrolysate adopts following method preparation:
1. brewer's yeast is added in the water of 15 times of weight ratios;
2. in the aqueous solution of brewer's yeast, add 0.5% dextranase, 1% papain, 1% cellulase;
3. adding the acetic acid adjust pH is 5.5;
4. under 50 ℃ temperature, reacted 4 hours;
5. the unreacted brewer's yeast of elimination;
6. concentration of reaction solution, vacuum drying obtains brown powder, wherein contains amino acid 70-80%, oligosaccharides 20-30%.
2. composite oligosaccharide plant growth stimulator according to claim 1 is characterized in that, comprises following component and content, and content is in weight %
Range of molecular weight distributions is at the chitosan oligomer 2% of 1000-5000
Range of molecular weight distributions is in the fructo-oligosaccharide (FOS) 2% of 2000-3000
Range of molecular weight distributions is at the oligomeric glucan 1% of 2000-3000
Brewer's yeast hydrolysate 10%
Trace element 0.5%
KH
2PO
410％
All the other are the chicken manure water extract, add to 100%.
3. the preparation method of composite oligosaccharide plant growth stimulator according to claim 1 and 2 is characterized in that, described preparation method's step is:
(1) preparation of brewer's yeast hydrolysate:
1. brewer's yeast is added among 15 times of weight ratio waters;
2. in the aqueous solution of brewer's yeast, add 0.5% dextranase, 1% papain, 1% cellulase;
3. adding the acetic acid adjust pH is 5.5;
4. under 50 ℃ temperature, reacted 4 hours;
5. the unreacted brewer's yeast of elimination;
6. concentration of reaction solution, vacuum drying obtains brown powder, wherein contains amino acid 70-80%, oligosaccharides 20-30%;
(2) with the brewer's yeast hydrolysate that obtains in the step (1) and chitosan oligomer, fructo-oligosaccharide (FOS), oligomeric glucan, trace element, KH
2PO
4, the chicken manure water extract mixes according to claim 1 or 2 described ratios, namely gets composite oligosaccharide plant growth stimulator.
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