CN101870604A - Composite oligosaccharide plant growth stimulator and preparation method thereof - Google Patents
Composite oligosaccharide plant growth stimulator and preparation method thereof Download PDFInfo
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- CN101870604A CN101870604A CN201010189079A CN201010189079A CN101870604A CN 101870604 A CN101870604 A CN 101870604A CN 201010189079 A CN201010189079 A CN 201010189079A CN 201010189079 A CN201010189079 A CN 201010189079A CN 101870604 A CN101870604 A CN 101870604A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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Abstract
The invention relates to a composite oligosaccharide plant growth stimulator and a preparation method thereof, wherein the composite oligosaccharide plant growth stimulator comprises the following components and contents (calculated by weight percent): 1-5% of oligomerization chitosan, 1-5% of fructo oligosaccharide, 1-2% of oligomerization glucan, 10% of beer yeast hydrolysate, 0.5-1% of trace element, 10% of KH2PO4 and the balance of chicken manure water extract, which add up to 100%. The preparation method comprises the steps of: (1) preparing beer yeast hydrolysate; and (2) mixing the beer yeast hydrolysate obtained in the step (1) with oligomerization chitosan, fructo oligosaccharide, oligomerization glucan, trace element, KH2PO4 and chicken manure water extract according to the ratio, and obtaining the composite oligosaccharide plant growth stimulator. The invention is non-toxic, harmless and environmental friendly, can stimulate the growth and prematurity of plants, improve the yield, can improve the stress resistance of the plants and the quality of medicinal materials, and has wide application scope.
Description
Technical field
The present invention relates to a kind of plant growth promoter and preparation method thereof, particularly a kind of plant growth promoter that contains composite oligosaccharides such as chitosan oligomer, oligofructose and oligomeric dextran, cereuisiae fermentum hydrolyzate and preparation method thereof.
Background technology
As everyone knows, quick growth along with the high speed development and the population of production technology, people require the hope of soil volume increase urgent day by day, the use of a large amount of chemical fertilizer and agricultural chemicals, harm to soil and farm crop is very serious, and the environment that people depend on for existence polluted, and harm people's health, modern scientific research shows, the growth of plant not only needs common nitrogen, phosphorus, potash fertilizer also needs necessary trace element, aminoacids complex and a certain amount of plant growth promoter, existing various promotion plant growing agents all can not maximizedly reach the best effect that promotes plant-growth, use the face of plant variety less, the effect of increasing production instability.
Summary of the invention
Technical problem to be solved by this invention provide a kind of non-pollution safety, use range green plants growth stimulant and preparation method thereof widely, its effect is by strengthening, regulate and control the activity of plant enzyme, the activation vegetable cell, the morphogenesis of inducing plant adventive root or indefinite bud, promote the formation and the growth of root system at short notice, regulate the plant metabolism action intensity, reach and improve plant growth rate, crop yield, increase nutrient content of plant and the purpose that improves disease-resistant performance.
The present invention solves the technical scheme that its technical problem takes: a kind of composite oligosaccharide plant growth stimulator, described composite oligosaccharide plant growth stimulator comprise following component and content (content is in weight %):
Chitosan oligomer (molecular-weight average 1000---5000) 1-5%,
Oligofructose (molecular weight 2000---3000) 1-5%, oligomeric dextran (2000-3000) 1-2%,
Cereuisiae fermentum hydrolyzate 10%, the trace element (Mo, B, mishmetal, Fe) 0.5-1%,
KH
2PO
410%, all the other add to 100% for the chicken manure water extract.
Wherein chitosan oligomer is by employing cellulase hydrolysis and the preparation of hydrogen peroxide degradation method, separates with ultra-filtration membrane, and spraying drying forms, and molecular weight detection adopts gel chromatography, and molecular weight ranges is 1000---5000.Oligofructose is that Radix Arctii extracts preparation.Oligomeric dextran is that beta glucan makes through the β enzymic hydrolysis.
The preparation method of composite oligosaccharide plant growth stimulator is as follows:
(1) preparation of cereuisiae fermentum hydrolyzate:
1. cereuisiae fermentum is added in 15 times of water (weight ratio); 2. in the aqueous solution of cereuisiae fermentum, add 0.5% dextranase, 1% papoid, 1% cellulase; 3. adding the acetic acid adjust pH is 5.5; 4. under 50 ℃ temperature, reacted 4 hours; 5. the unreacted cereuisiae fermentum of elimination; 6. concentration of reaction solution, vacuum-drying obtains brown powder, wherein contains amino acid 70-80%, oligosaccharides 20-30%.
(2) with the cereuisiae fermentum hydrolyzate that obtains in the step (1) and chitosan oligomer, oligofructose, oligomeric dextran, trace element (Mo, B, mishmetal, Fe), KH
2PO
4, the chicken manure water extract evenly promptly gets composite oligosaccharide plant growth stimulator according to above-described mixed.
The proportioning mode of optimum of the present invention further is:
Chitosan oligomer (molecular-weight average 1000---5000) 2%,
Oligofructose (molecular weight 2000---3000) 2%, oligomeric dextran (2000-3000) 1%,
Cereuisiae fermentum hydrolyzate 10%, the trace element (Mo, B, mishmetal, Fe) 0.5%,
KH
2PO
410%, all the other add to 100% for the chicken manure water extract.
The preparation method of composite oligosaccharide plant growth stimulator of the present invention is as follows:
Sieve below above-mentioned each component solid material is crushed to 100 orders, carry out thorough mixing according to optimization formula in the solid mixer, sealing gets final product in the plastics bag of packing into.
Using method: composite oligosaccharide plant growth stimulator 10 grams are dissolved in 20 kg of water, can spray with root plant with this solution and irritate.
Composite oligosaccharide plant growth stimulator of the present invention has the following advantages:
1, all nontoxic, the environmental protection of all components, harmless, be applicable to that the raw material planting of green food is used, be a kind of safe green plants growth stimulant.
2, composite oligosaccharide plant growth stimulator of the present invention contains the nutritive substance such as oligosaccharide, multiple amino acids, various trace elements and nitrogen, phosphorus, potassium of multiple promotion plant-growth.Can promote the growth of root system of plant and cauline leaf.Should short long agent can improve the plant water-absorbent, promote photosynthesis, can increase substantially crop yield, and generally can increase production 10-30%.Improve nutritive ingredient in the plant, increase the mouthfeel and the outward appearance of fruit.
3, this promotor can be improved the anti-adversity of plant, for example: improve plant drought, cold-resistant, disease-resistant, resistant to lodging performance.This product can be secreted a large amount of plant protecting chemicals by stimulating plant, improves stress resistance of plant, comprises the bacteriostasis property that improves plant.Therefore, this product can to a certain degree play control iron rust disease, prevent and treat Powdery Mildew etc.
4, this product can improve the active constituent content in the Chinese medicinal plant, improves the Chinese medicinal materials quality.As: improve the content of astragalus polysaccharides content, raising tanshinone IIA, the content of raising Potenlini etc., main because the oligosaccharides in this product can accumulate secondary metabolites generation plant protecting chemical and polysaccharide by stimulating plant.
5, this plant promoting agent can promote jarovize, and for example corn, paddy rice, leafy vegetable etc. generally can shift to an earlier date 7-10 days ripe results.
6, this plant growth promoter has wide range of applications, applicable to various leafy vegetables, melon and fruit, rhizome vegetables, plant medicine material, flowers, wheat, paddy rice, soybean, tealeaves, tree cuttage etc.
Embodiment
Experimental results show that chitosan oligomer, oligofructose and oligomeric dextran etc. have the effect that promotes plant-growth and improve the plant stress-resistance performance, by strengthening, regulate and control the activity of plant enzyme, the activation vegetable cell, promote the synthetic of biomacromolecule, the morphogenesis of inducing plant adventive root or indefinite bud, promote the formation and the growth of root system at short notice, regulate the plant metabolism action intensity, reach and improve plant growth rate, crop yield, increase nutrient content of plant and the purpose that improves disease-resistant performance.Composite oligosaccharide is stronger than the effect of single oligose, and cereuisiae fermentum produces oligomeric dextran, oligopeptides and multiple amino acids by multiple enzymolysis, and plant-growth is had promoter action.The plant growth promoter of forming by composite oligosaccharide, cereuisiae fermentum hydrolyzate and various trace elements does not contain the synthetic hormone, environment is not polluted, and not producing residually, safe in utilization in farm crop, is a kind of novel green plant growth promoter.Below be illustrated with embodiment.
Embodiment 1 composite oligosaccharide plant growth stimulator is to romaine lettuce growth and yield effect
Composite oligosaccharide plant growth stimulator is added the ratio that 20 kg of water and 10 grams add 30 kg of water according to 10 grams be mixed with solution, carry out control experiment with clear water respectively, the romaine lettuce seed is soaked soaked in three hours, sowing at the beginning of 9 months.20 strains of taking a sample respectively in the 4th day, the 8th day, the 11st day investigation plant maximum root, the horizontal stem of maximum root, the test of taking root.
The aqueous solution (containing the clear water contrast) for preparing above-mentioned promotor is sprayed processing to romaine lettuce, spray behind 5 leaves when seedling is long, sprayed once in per 7 days, the whole growth cycle handles 3 times with promotor.
The romaine lettuce seed soaks the back percentage of germination through accelerator solution and obviously improves, and seedling is irritated length and the root diameter of executing the back root through the promotor root obviously to be increased, and sprays this plant growth promoter in the vegetative period of romaine lettuce, and plant strain growth is accelerated, blade increases.Through after spraying, the chlorophyll of romaine lettuce, vitamins C, reducing sugar content all increase, and per mu yield increases by 8%.After the romaine lettuce seed soaked composite oligosaccharide plant growth stimulator, the growing state of root saw Table 1.
Table 1: after the romaine lettuce seed soaks composite oligosaccharide plant growth stimulator, the growing state of root
Spraying back 5 days of processing, random sampling 10 strains investigation biological character the results are shown in Table 2 at every turn.
Table 2: biological character questionnaire
After spraying through three times as can be seen from Table 2,0.03% concentration group plant height, the number of blade, maximum leaf are grown, leaf Da Ye is wide all apparently higher than the clear water group, illustrate that composite oligosaccharide plant growth stimulator can promote the romaine lettuce growth.
Table 3: composite oligosaccharide plant growth stimulator is handled back romaine lettuce output statistics table
Unit: Kg
After handling through composite oligosaccharide plant growth stimulator as can be seen from Table 3, all increase than control group per mu yield, wherein 0.03% group of volume increase is the most remarkable.
Embodiment 2 composite oligosaccharide plant growth stimulators are to romaine lettuce growth and yield effect
The test cucumber is adopted and extensively grinds No. 3
Composite oligosaccharide plant growth stimulator is added the ratio that 20 kg of water and 10 grams add 30 kg of water according to 10 grams be mixed with solution, carry out control experiment with clear water respectively, cucumber seeds is soaked soaked in three hours, sowing at the beginning of 9 months.Got sheep 20 strains investigation plant maximum root, the horizontal stem of maximum root in the 3rd day, the 6th day, the 9th day respectively, the test of taking root.
Moonrise at 2007 9 sows, and promotes growth test, establishes three groups, three times and repeats, totally 18 districts, 20 square metres of each sub-district areas.Experimental result is as follows:
Table 4: after composite oligosaccharide plant growth stimulator soaks seed, to the thick influence of root
As can be seen from Table 4, behind composite oligosaccharide plant growth stimulator immersion seed, the root of cucumber slightly all is higher than the clear water group.
Table 5: after composite oligosaccharide plant growth stimulator soaks seed, the influence long to root
Mountain table 5 as can be seen, soak seed through composite oligosaccharide plant growth stimulator after, the long clear water group that all is higher than of the root of cucumber.Above presentation of results composite oligosaccharide plant growth stimulator has obvious promoter action to the root growth of cucumber.
Table 6: composite oligosaccharide plant growth stimulator is handled the influence of back cucumber aerial growth
As can be seen from Table 6, after the process composite oligosaccharide plant growth stimulator sprays processing, the plant height of cucumber, the long and wide control group that all is higher than of leaf Da Ye of climing thick, maximum leaf, it is best that wherein 0.03% concentration group is handled thick long-acting fruit, illustrates that composite oligosaccharide plant growth stimulator has obvious facilitation to the cucumber aerial growth.
Table 7: composite oligosaccharide plant growth stimulator is handled back cucumber yield cartogram
Unit: Kg
Cucumber per mu yield and eurymeric melon rate all are significantly increased after handling through composite oligosaccharide plant growth stimulator as can be seen from Table 7.
Claims (3)
1. a composite oligosaccharide plant growth stimulator is characterized in that, described composite oligosaccharide plant growth stimulator comprises following component and content (content is in weight %):
Chitosan oligomer (molecular-weight average 1000---5000) 1-5%,
Oligofructose (molecular weight 2000---3000) 1-5%, oligomeric dextran (2000-3000) 1-2%,
Cereuisiae fermentum hydrolyzate 10%, the trace element (Mo, B, mishmetal, Fe) 0.5-1%,
KH
2PO
410%, all the other add to 100% for the chicken manure water extract.
2. composite oligosaccharide plant growth stimulator according to claim 1 is characterized in that, the composition and the content of described plant growth promoter are as follows:
Chitosan oligomer (molecular-weight average 1000---5000) 2%,
Oligofructose (molecular weight 2000---3000) 2%, oligomeric dextran (2000-3000) 1%,
Cereuisiae fermentum hydrolyzate 10%, the trace element (Mo, B, mishmetal, Fe) 0.5%,
KH
2PO
410%, all the other add to 100% for the chicken manure water extract.
3. the preparation method of composite oligosaccharide plant growth stimulator according to claim 1 and 2 is characterized in that, preparation method's step is:
(1) preparation of cereuisiae fermentum hydrolyzate:
1. cereuisiae fermentum is added in 15 times of water (weight ratio); 2. in the aqueous solution of cereuisiae fermentum, add 0.5% dextranase, 1% papoid, 1% cellulase; 3. adding the acetic acid adjust pH is 5.5; 4. under 50 ℃ temperature, reacted 4 hours; 5. the unreacted cereuisiae fermentum of elimination; 6. concentration of reaction solution, vacuum-drying obtains brown powder, wherein contains amino acid 70-80%, oligosaccharides 20-30%.
(2) with the cereuisiae fermentum hydrolyzate that obtains in the step (1) and chitosan oligomer, oligofructose, oligomeric dextran, trace element (Mo, B, mishmetal, Fe), KH
2PO
4, the chicken manure water extract evenly promptly gets composite oligosaccharide plant growth stimulator according to claim 1 or 2 described mixed.
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| CN2010101890799A CN101870604B (en) | 2010-06-02 | 2010-06-02 | Composite oligosaccharide plant growth stimulator and preparation method thereof |
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| CN2010101890799A CN101870604B (en) | 2010-06-02 | 2010-06-02 | Composite oligosaccharide plant growth stimulator and preparation method thereof |
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|---|---|
| CN101870604A true CN101870604A (en) | 2010-10-27 |
| CN101870604B CN101870604B (en) | 2013-09-11 |
Family
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Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102144637A (en) * | 2010-12-23 | 2011-08-10 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide composite with cold-resistant function and application of composite |
| CN102144639A (en) * | 2010-12-23 | 2011-08-10 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide composite with drought resisting function and application of composite |
| CN102613189A (en) * | 2012-03-07 | 2012-08-01 | 海南正业中农高科股份有限公司 | Application of chitosan oligosaccharide in promoting early ripening of crops |
| CN102992866A (en) * | 2012-10-10 | 2013-03-27 | 肥西县农业技术推广中心 | Preparation method of rape specific pesticide fertilizer containing vinegar prepared Chinese medicaments |
| CN104150977A (en) * | 2014-07-25 | 2014-11-19 | 国家海洋局第三海洋研究所 | Preparation method and application of alga oligosaccharide biological fertilizer |
| CN104150973A (en) * | 2013-12-17 | 2014-11-19 | 广西金臣科技有限公司 | Organic fertilizer additive and preparation method thereof |
| CN104450522A (en) * | 2013-09-22 | 2015-03-25 | 中国科学院天津工业生物技术研究所 | Beer yeast cell disruption method adopting synergetic enzyme and mechanical disruption |
| CN105010397A (en) * | 2015-06-11 | 2015-11-04 | 高青松 | Formulation for protecting plants in dry-hot wind |
| CN105410034A (en) * | 2015-11-27 | 2016-03-23 | 湖北工业大学 | Compound promoter for cordyceps sinensis cultivation and preparation method thereof |
| CN105481563A (en) * | 2015-12-23 | 2016-04-13 | 成都新柯力化工科技有限公司 | Synergistic ecological fertilizer special for radix astragali and preparation method of synergistic ecological fertilizer |
| CN105969377A (en) * | 2016-02-19 | 2016-09-28 | 含山县丰华供销合作社有限公司 | Clayey soil conditioner |
| CN105967936A (en) * | 2016-02-19 | 2016-09-28 | 含山县丰华供销合作社有限公司 | Molybdenum-tailings-improved soil mineral remediation agent |
| CN107279182A (en) * | 2017-07-27 | 2017-10-24 | 四川国光农化股份有限公司 | A kind of plant growth regulator composition, preparation and its application |
| CN107382539A (en) * | 2017-09-06 | 2017-11-24 | 汪建利 | Improve air ambient and promote biological agent of photosynthesis of plant and preparation method thereof |
| CN107518009A (en) * | 2017-07-14 | 2017-12-29 | 北京百丰天下生物科技有限公司 | Clear garden bacteria agent applied to germ cleaning and preparation method thereof |
| CN108048097A (en) * | 2017-12-26 | 2018-05-18 | 中国科学院烟台海岸带研究所 | A kind of seaweeds soil beneficial microbe accelerating agent |
| CN113367138A (en) * | 2021-07-17 | 2021-09-10 | 湖南省水稻研究所 | Ecological pesticide for organic rice planting |
| CN113993379A (en) * | 2019-06-17 | 2022-01-28 | 昭和电工株式会社 | Plant vigor agent comprising amino acid or salt thereof, and oligosaccharide, and use thereof |
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Cited By (24)
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|---|---|---|---|---|
| CN102144639A (en) * | 2010-12-23 | 2011-08-10 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide composite with drought resisting function and application of composite |
| CN102144639B (en) * | 2010-12-23 | 2012-07-04 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide composite with drought resisting function and application of composite |
| CN102144637B (en) * | 2010-12-23 | 2012-07-04 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide composite with cold-resistant function and application of composite |
| CN102144637A (en) * | 2010-12-23 | 2011-08-10 | 海南正业中农高科股份有限公司 | Chitosan oligosaccharide composite with cold-resistant function and application of composite |
| CN102613189A (en) * | 2012-03-07 | 2012-08-01 | 海南正业中农高科股份有限公司 | Application of chitosan oligosaccharide in promoting early ripening of crops |
| CN102992866A (en) * | 2012-10-10 | 2013-03-27 | 肥西县农业技术推广中心 | Preparation method of rape specific pesticide fertilizer containing vinegar prepared Chinese medicaments |
| CN104450522B (en) * | 2013-09-22 | 2017-04-12 | 中国科学院天津工业生物技术研究所 | Beer yeast cell disruption method adopting synergetic enzyme and mechanical disruption |
| CN104450522A (en) * | 2013-09-22 | 2015-03-25 | 中国科学院天津工业生物技术研究所 | Beer yeast cell disruption method adopting synergetic enzyme and mechanical disruption |
| CN104150973B (en) * | 2013-12-17 | 2015-12-30 | 广西金臣科技有限公司 | Organic fertilizer additive and preparation method thereof |
| CN104150973A (en) * | 2013-12-17 | 2014-11-19 | 广西金臣科技有限公司 | Organic fertilizer additive and preparation method thereof |
| CN104150977A (en) * | 2014-07-25 | 2014-11-19 | 国家海洋局第三海洋研究所 | Preparation method and application of alga oligosaccharide biological fertilizer |
| CN104150977B (en) * | 2014-07-25 | 2017-02-15 | 国家海洋局第三海洋研究所 | Preparation method and application of alga oligosaccharide biological fertilizer |
| CN105010397A (en) * | 2015-06-11 | 2015-11-04 | 高青松 | Formulation for protecting plants in dry-hot wind |
| CN105410034A (en) * | 2015-11-27 | 2016-03-23 | 湖北工业大学 | Compound promoter for cordyceps sinensis cultivation and preparation method thereof |
| CN105481563A (en) * | 2015-12-23 | 2016-04-13 | 成都新柯力化工科技有限公司 | Synergistic ecological fertilizer special for radix astragali and preparation method of synergistic ecological fertilizer |
| CN105967936A (en) * | 2016-02-19 | 2016-09-28 | 含山县丰华供销合作社有限公司 | Molybdenum-tailings-improved soil mineral remediation agent |
| CN105969377A (en) * | 2016-02-19 | 2016-09-28 | 含山县丰华供销合作社有限公司 | Clayey soil conditioner |
| CN107518009A (en) * | 2017-07-14 | 2017-12-29 | 北京百丰天下生物科技有限公司 | Clear garden bacteria agent applied to germ cleaning and preparation method thereof |
| CN107279182A (en) * | 2017-07-27 | 2017-10-24 | 四川国光农化股份有限公司 | A kind of plant growth regulator composition, preparation and its application |
| CN107279182B (en) * | 2017-07-27 | 2020-11-24 | 四川国光农化股份有限公司 | Plant growth regulator composition, preparation and application thereof |
| CN107382539A (en) * | 2017-09-06 | 2017-11-24 | 汪建利 | Improve air ambient and promote biological agent of photosynthesis of plant and preparation method thereof |
| CN108048097A (en) * | 2017-12-26 | 2018-05-18 | 中国科学院烟台海岸带研究所 | A kind of seaweeds soil beneficial microbe accelerating agent |
| CN113993379A (en) * | 2019-06-17 | 2022-01-28 | 昭和电工株式会社 | Plant vigor agent comprising amino acid or salt thereof, and oligosaccharide, and use thereof |
| CN113367138A (en) * | 2021-07-17 | 2021-09-10 | 湖南省水稻研究所 | Ecological pesticide for organic rice planting |
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