CN108048097A - A kind of seaweeds soil beneficial microbe accelerating agent - Google Patents
A kind of seaweeds soil beneficial microbe accelerating agent Download PDFInfo
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- CN108048097A CN108048097A CN201711433440.6A CN201711433440A CN108048097A CN 108048097 A CN108048097 A CN 108048097A CN 201711433440 A CN201711433440 A CN 201711433440A CN 108048097 A CN108048097 A CN 108048097A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
- C09K17/18—Prepolymers; Macromolecular compounds
- C09K17/32—Prepolymers; Macromolecular compounds of natural origin, e.g. cellulosic materials
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
Abstract
The invention discloses a kind of seaweeds soil beneficial microbe accelerating agents, are as made from the raw material of following parts by weight：100 120 parts of alginic acid, 60 80 parts of chitosan oligosaccharide, 10 20 parts of inulin, 5 10 parts of oligofructose, 35 parts of poly-D-lysine, 24 parts of garlic polysaccharide, 5 10 parts of algal polysaccharides, 0.5 part of cysteine.Seaweeds soil beneficial microbe of the present invention promotes agent prescription reasonable, and each component synergistic effect can be good at promoting the increase of beneficial microbe colony in soil, form rational soil Bacterial community.
Description
Technical field
The invention belongs to technical field of land improvement, and in particular to a kind of seaweeds soil beneficial microbe accelerating agent.
Background technology
Soil is that the mankind rely one of main resource of existence and an important part of the human ecological environment.Soil bag
Containing 45% inorganic matter, 5% organic matter, 25% moisture and 25% air.Soil is typically what is be layered, and uppermost one layer is
Surface layer, wherein the animal containing humus, the root of plant and work（Such as microorganism and earthworm）, humus is more, and topsoil is more fertile
It is fertile.On the ground of some such as forests, there is many humus and form a separation layer.Under being under topsoil
Layer soil, the clay ratio bigger that may be included, the organic matter contained are less.It is decayed rock under subsoil, then down
It is hard sill.
Soil texture is solid phase of soil particle（Including aggregate）Size and its space arrangement form, not only influence
The reserves and deliverability of soil moisture needed for plant growth and nutrient, and also affect gas exchange, heat in soil and put down
Weighing apparatus, microbial activities and extension of root system etc..
Soil is an open system, so surviving in the soil there are many kinds of microorganism, with their works to plant
It is divided into two major class of beneficial microbe colony and disease microorganism species with relation.Beneficial microbe colony is the main force in soil
Main forces, they can resolve into plant by the organic matter in soil and inorganic salts under the action of the metabolite enzyme of their own
The required protein of object and amino acid, at the same their own breeding and metabolite be also a kind of protein and amino acid,
The presence of these substances is formed protein small-sized one by one and amino acid processing factory in plant root, protects plant health
Growth.The foundation and maintenance of beneficial microbe colony, can promote the performance of root growth and its function, if on the contrary, disease
Microorganism species occupy leading position, then root system development is bad, and root function is damaged, and overground part grows suppressed, crop yield
And quality decline.
The content of the invention
The present invention provides a kind of seaweeds soil beneficial microbe accelerating agents, can be good at promoting in soil beneficial to micro-
The increase of biological flora forms rational soil Bacterial community.
The purpose of the present invention is what is be achieved through the following technical solutions：
A kind of seaweeds soil beneficial microbe accelerating agent, is as made from the raw material of following parts by weight：Alginic acid 100-120
Part, 60-80 parts of chitosan oligosaccharide, 10-20 parts of inulin, 5-10 parts of oligofructose, 3-5 parts of poly-D-lysine, 2-4 parts of garlic polysaccharide, sea
5-10 parts of polysaccharides, 0.5 part of cysteine.
Preferably, a kind of seaweeds soil beneficial microbe accelerating agent, is as made from the raw material of following parts by weight：Seaweed
110 parts of acid, 70 parts of chitosan oligosaccharide, 15 parts of inulin, 8 parts of oligofructose, 4 parts of poly-D-lysine, 3 parts of garlic polysaccharide, algal polysaccharides 8
Part, 0.5 part of cysteine.
The molecular weight of the alginic acid is 100KDa-150KDa.
The garlic polysaccharide is prepared by following steps：
1）Fresh garlic cleans up, and removes garlic skin, crushes, obtains garlic slurries；
2）The water of 6 times of quality is added in into garlic slurries, 90 DEG C of boiling 1h keep the temperature 0.5h, cooled to room temperature, and centrifugation takes
Clear liquid；
3）By supernatant through the ultrafiltration membrance filter that molecular weight is 10KDa-20KDa, crude extract is obtained；
4）Crude extract is adjusted into pH to neutrality, through activated carbon decolorizing, 20min is kept the temperature at 70 DEG C, obtains extracting solution；
5）It is spray-dried after 60 DEG C of extracting solution is concentrated under reduced pressure, obtains garlic polysaccharide.
The sulfate radical content of the algal polysaccharides is≤30%, molecular weight 50KDa-100KDa.
Beneficial effects of the present invention：Seaweeds soil beneficial microbe of the present invention promotes agent prescription reasonable, each component collaboration
Effect can be good at promoting the increase of beneficial microbe colony in soil, form rational soil Bacterial community；Play flora
Advantage improves soil fertilizer matter, forms good crop growth environment, promotes well developed root system, cell is promoted to increase, division, nucleic acid
With the synthesis of protein, ensure supply of the root to aerial part moisture and nutrient and basic element of cell division isoreactivity substance, favorably
In the enhancing of above-ground plant parts normal activity, crop yield is significantly improved；Inhibit pathogenic microbes, reduce the hair of pest and disease damage
It is raw.
Specific embodiment
Illustrate the present invention referring to specific embodiment.It will be appreciated by those skilled in the art that these embodiments are only
For illustrating the present invention, do not limit the scope of the invention in any way.
The experimental method of following embodiments is conventional method unless otherwise specified.Original used in following embodiments
Material, reagent material etc. unless otherwise specified, are commercially available products.
The degree of polymerization of chitosan oligosaccharide of the present invention is between 2-20, molecular weight≤3200Da.
The molecular weight of poly-D-lysine of the present invention is more than 3KDa.
Embodiment 1
A kind of seaweeds soil beneficial microbe accelerating agent, is as made from the raw material of following parts by weight：110 parts of alginic acid, shell
70 parts of oligosaccharides, 15 parts of inulin, 8 parts of oligofructose, 4 parts of poly-D-lysine, 3 parts of garlic polysaccharide, 8 parts of algal polysaccharides, cysteine
0.5 part.
The molecular weight of the alginic acid is 100KDa-150KDa.
The garlic polysaccharide is prepared by following steps：
1）Fresh garlic cleans up, and removes garlic skin, crushes, obtains garlic slurries；
2）The water of 6 times of quality is added in into garlic slurries, 90 DEG C of boiling 1h keep the temperature 0.5h, cooled to room temperature, and centrifugation takes
Clear liquid；
3）By supernatant through the ultrafiltration membrance filter that molecular weight is 10KDa-20KDa, crude extract is obtained；
4）Crude extract is adjusted into pH to neutrality, through activated carbon decolorizing, 20min is kept the temperature at 70 DEG C, obtains extracting solution；
5）It is spray-dried after 60 DEG C of extracting solution is concentrated under reduced pressure, obtains garlic polysaccharide.
The sulfate radical content of the algal polysaccharides is≤30%, molecular weight 50KDa-100KDa.
Embodiment 2
A kind of seaweeds soil beneficial microbe accelerating agent, is as made from the raw material of following parts by weight：100 parts of alginic acid, shell
80 parts of oligosaccharides, 20 parts of inulin, 10 parts of oligofructose, 3 parts of poly-D-lysine, 2 parts of garlic polysaccharide, 5 parts of algal polysaccharides, cysteine
0.5 part.
The molecular weight of the alginic acid is 100KDa-150KDa.
The garlic polysaccharide is prepared by following steps：
1）Fresh garlic cleans up, and removes garlic skin, crushes, obtains garlic slurries；
2）The water of 6 times of quality is added in into garlic slurries, 90 DEG C of boiling 1h keep the temperature 0.5h, cooled to room temperature, and centrifugation takes
Clear liquid；
3）By supernatant through the ultrafiltration membrance filter that molecular weight is 10KDa-20KDa, crude extract is obtained；
4）Crude extract is adjusted into pH to neutrality, through activated carbon decolorizing, 20min is kept the temperature at 70 DEG C, obtains extracting solution；
5）It is spray-dried after 60 DEG C of extracting solution is concentrated under reduced pressure, obtains garlic polysaccharide.
The sulfate radical content of the algal polysaccharides is≤30%, molecular weight 50KDa-100KDa.
Embodiment 3
A kind of seaweeds soil beneficial microbe accelerating agent, is as made from the raw material of following parts by weight：120 parts of alginic acid, shell
60 parts of oligosaccharides, 10 parts of inulin, 5 parts of oligofructose, 5 parts of poly-D-lysine, 4 parts of garlic polysaccharide, 10 parts of algal polysaccharides, cysteine
0.5 part.
The molecular weight of the alginic acid is 100KDa-150KDa.
The garlic polysaccharide is prepared by following steps：
1）Fresh garlic cleans up, and removes garlic skin, crushes, obtains garlic slurries；
2）The water of 6 times of quality is added in into garlic slurries, 90 DEG C of boiling 1h keep the temperature 0.5h, cooled to room temperature, and centrifugation takes
Clear liquid；
3）By supernatant through the ultrafiltration membrance filter that molecular weight is 10KDa-20KDa, crude extract is obtained；
4）Crude extract is adjusted into pH to neutrality, through activated carbon decolorizing, 20min is kept the temperature at 70 DEG C, obtains extracting solution；
5）It is spray-dried after 60 DEG C of extracting solution is concentrated under reduced pressure, obtains garlic polysaccharide.
The sulfate radical content of the algal polysaccharides is≤30%, molecular weight 50KDa-100KDa.
Comparative example 1
A kind of seaweeds soil beneficial microbe accelerating agent, is as made from the raw material of following parts by weight：110 parts of alginic acid, chrysanthemum
15 parts of powder, 8 parts of oligofructose, 4 parts of poly-D-lysine, 3 parts of garlic polysaccharide, 8 parts of algal polysaccharides, 0.5 part of cysteine.
The molecular weight of the alginic acid is 100KDa-150KDa.
The garlic polysaccharide is prepared by following steps：
1）Fresh garlic cleans up, and removes garlic skin, crushes, obtains garlic slurries；
2）The water of 6 times of quality is added in into garlic slurries, 90 DEG C of boiling 1h keep the temperature 0.5h, cooled to room temperature, and centrifugation takes
Clear liquid；
3）By supernatant through the ultrafiltration membrance filter that molecular weight is 10KDa-20KDa, crude extract is obtained；
4）Crude extract is adjusted into pH to neutrality, through activated carbon decolorizing, 20min is kept the temperature at 70 DEG C, obtains extracting solution；
5）It is spray-dried after 60 DEG C of extracting solution is concentrated under reduced pressure, obtains garlic polysaccharide.
The sulfate radical content of the algal polysaccharides is≤30%, molecular weight 50KDa-100KDa.
Comparative example 2
A kind of seaweeds soil beneficial microbe accelerating agent, is as made from the raw material of following parts by weight：110 parts of alginic acid, shell
70 parts of oligosaccharides, 15 parts of inulin, 8 parts of oligofructose, 3 parts of garlic polysaccharide, 8 parts of algal polysaccharides, 0.5 part of cysteine.
The molecular weight of the alginic acid is 100KDa-150KDa.
The garlic polysaccharide is prepared by following steps：
1）Fresh garlic cleans up, and removes garlic skin, crushes, obtains garlic slurries；
2）The water of 6 times of quality is added in into garlic slurries, 90 DEG C of boiling 1h keep the temperature 0.5h, cooled to room temperature, and centrifugation takes
Clear liquid；
3）By supernatant through the ultrafiltration membrance filter that molecular weight is 10KDa-20KDa, crude extract is obtained；
4）Crude extract is adjusted into pH to neutrality, through activated carbon decolorizing, 20min is kept the temperature at 70 DEG C, obtains extracting solution；
5）It is spray-dried after 60 DEG C of extracting solution is concentrated under reduced pressure, obtains garlic polysaccharide.
The sulfate radical content of the algal polysaccharides is≤30%, molecular weight 50KDa-100KDa.
Comparative example 3
A kind of seaweeds soil beneficial microbe accelerating agent, is as made from the raw material of following parts by weight：110 parts of alginic acid, shell
70 parts of oligosaccharides, 15 parts of inulin, 8 parts of oligofructose, 4 parts of poly-D-lysine, 0.5 part of cysteine.
The molecular weight of the alginic acid is 100KDa-150KDa.
Culture medium bacterium colony is tested
The embodiment 1-3 and comparative example 1-3 seaweeds soil beneficial microbe accelerating agents prepared are respectively added in culture medium,
Culture medium specifically comprises：Yeast extract 50g, mannitol 80g, agar 50g, seaweeds soil beneficial microbe accelerating agent 20g.
By the streak inoculation on above-mentioned 6 culture mediums, and 45h is cultivated under the conditions of 32-37 DEG C respectively of nitrogen-fixing bacteria, agrobacterium, culture
After finishing, observe and count the single bacterium colony average diameter of two kinds of bacterium on culture medium, concrete outcome is shown in Table 1.
1 culture medium bacterium colony result of the test of table
The nitrogen-fixing bacteria be blown-ball Azotobacter, preserving number CICC21686；The agrobacterium be Agrobacterium tumefaciens, preservation
It is commercially available strain number for ACCC 10601.
Cotton field experiment
Select that 7 pieces of soil properties are consistent, fertility is uniformly medium, and cotton wilt and verticillium wilt are fallen ill heavier cotton field, grouping
For experimental plot 1-6 and control field, wherein experimental plot 1-3 applies seaweeds soil beneficial microbe prepared by embodiment 1-3 respectively
100kg/ mus of 25kg/ mus+common fertilizer of accelerating agent, it is beneficial that experimental plot 4-6 applies seaweeds soil prepared by comparative example 1-3 respectively
125kg/ mus of common fertilizer is applied in 100kg/ mus of 25kg/ mus+common fertilizer of microorganism accelerating agent, control field.
Each experimental plot uses ten point samplings, 100 plants is sampled per experimental plot, fertilising withered to cotton wilt and Huang after 50 days
Disease is investigated, and is recorded and is counted incidence and control rate, the results are shown in Table 2.The computational methods of incidence and control rate are such as
Under：
Incidence（%）=morbidity strain number/investigation total strain number × 100；
Control rate（%）=（Compare field incidence-experimental plot incidence）/ control field incidence × 100.
After the harvest of cotton field, cottonseed yield is recorded, and calculates rate of growth, computational methods are as follows：
Rate of growth（%）=（Experimental plot seed cotton yield-control field seed cotton yield）/ control field seed cotton yield × 100.
2 cotton field experiment result of table
The above is only the preferred embodiment of the present invention, it should be pointed out that：Come for those skilled in the art
It says, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (5)
1. a kind of seaweeds soil beneficial microbe accelerating agent, which is characterized in that be as made from the raw material of following parts by weight：Sea
100-120 parts of alginic acid, 60-80 parts of chitosan oligosaccharide, 10-20 parts of inulin, 5-10 parts of oligofructose, 3-5 parts of poly-D-lysine, garlic are more
2-4 parts sugared, 5-10 parts of algal polysaccharides, 0.5 part of cysteine.
2. seaweeds soil beneficial microbe accelerating agent according to claim 1, which is characterized in that be by following parts by weight
Made from raw material：110 parts of alginic acid, 70 parts of chitosan oligosaccharide, 15 parts of inulin, 8 parts of oligofructose, 4 parts of poly-D-lysine, garlic polysaccharide
3 parts, 8 parts of algal polysaccharides, 0.5 part of cysteine.
3. seaweeds soil beneficial microbe accelerating agent according to claim 1 or claim 2, which is characterized in that the alginic acid
Molecular weight is 100KDa-150KDa.
4. seaweeds soil beneficial microbe accelerating agent according to claim 1 or claim 2, which is characterized in that the garlic polysaccharide
It is prepared by following steps：
1）Fresh garlic cleans up, and removes garlic skin, crushes, obtains garlic slurries；
2）The water of 6 times of quality is added in into garlic slurries, 90 DEG C of boiling 1h keep the temperature 0.5h, cooled to room temperature, and centrifugation takes
Clear liquid；
3）By supernatant through the ultrafiltration membrance filter that molecular weight is 10KDa-20KDa, crude extract is obtained；
4）Crude extract is adjusted into pH to neutrality, through activated carbon decolorizing, 20min is kept the temperature at 70 DEG C, obtains extracting solution；
5）It is spray-dried after 60 DEG C of extracting solution is concentrated under reduced pressure, obtains garlic polysaccharide.
5. seaweeds soil beneficial microbe accelerating agent according to claim 1 or claim 2, which is characterized in that the algal polysaccharides
Sulfate radical content for≤30%, molecular weight 50KDa-100KDa.
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Cited By (3)
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| CN109734521A (en) * | 2019-03-18 | 2019-05-10 | 赤峰学院 | A kind of fertilizing method for vegetables mineral nutrition balance |
| CN110819359A (en) * | 2019-11-22 | 2020-02-21 | 山东寡糖谷生物科技有限公司 | Method for preparing composite oligosaccharide soil remediation agent |
| CN114395607A (en) * | 2022-03-28 | 2022-04-26 | 卡力(烟台)农业发展有限公司 | Seaweed oligopeptide powder and preparation method thereof |
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