CN107188715A - A kind of environment protection type multifunctional liquid fertilizer and preparation method thereof - Google Patents
A kind of environment protection type multifunctional liquid fertilizer and preparation method thereof Download PDFInfo
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- CN107188715A CN107188715A CN201710540102.6A CN201710540102A CN107188715A CN 107188715 A CN107188715 A CN 107188715A CN 201710540102 A CN201710540102 A CN 201710540102A CN 107188715 A CN107188715 A CN 107188715A
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- 235000010755 mineral Nutrition 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 239000006012 monoammonium phosphate Substances 0.000 description 1
- 235000019837 monoammonium phosphate Nutrition 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
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- 235000021049 nutrient content Nutrition 0.000 description 1
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- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000008557 oxygen metabolism Effects 0.000 description 1
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- 235000020232 peanut Nutrition 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000011941 photocatalyst Substances 0.000 description 1
- 230000007180 physiological regulation Effects 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
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- 239000004016 soil organic matter Substances 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of environment protection type multifunctional liquid fertilizer and preparation method thereof.The environment protection type multifunctional liquid fertilizer of the present invention is to be prepared from by 400 700 parts of a great number of elements, middle micro- 50 100 parts, 30 parts of EDTA10,1 10 parts of part, nanometer the titanium carbon complex liquid of Nano Silver complex liquid 0.1 1,2 15 parts of composite reactive polypeptide protein enzyme liquid, 5 15 parts of single cell protein micro-capsule, 250 450 parts of water according to weight proportion.The environment protection type multifunctional liquid fertilizer of the present invention has the advantages that nutrition is balanced, absorption rate is high, preventing and treating plant pest, enhancing plant immune, increase yield, improving quality, improved soil, the technology of the present invention and application range of products are wide, chemical fertilizer and poisonous and harmful pesticide dosage can be reduced, realize the integration of medicine fertilizer, water-fertilizer integral, it is safe and efficient, environmental protection.
Description
Technical Field
The invention relates to an environment-friendly multifunctional liquid fertilizer, in particular to a novel multifunctional liquid fertilizer which is prepared from macroelements, medium-trace elements, EDTA (ethylene diamine tetraacetic acid), single-cell protein microcapsules, nano-silver compound liquid, nano-titanium-carbon compound liquid, compound active polypeptide protease liquid and water, has balanced nutrition and high absorption and utilization rate, prevents and treats plant diseases and insect pests, enhances plant immunity, increases yield, improves quality and improves soil, and a preparation method thereof, and is applied to the field of agriculture.
Background
At present, the usage amount of chemical fertilizers and pesticides in China is quite large, the production and usage amount is the first in the world, but the utilization rate of the chemical fertilizers and the pesticides is 15% -20% lower than that of developed countries, and the usage amount is reduced and the utilization rate is improved. Meanwhile, the unreasonable use of chemical fertilizers and pesticides seriously damages the immune system of plants, reduces the resistance and induces various plant diseases and insect pests. The long-term unreasonable use of chemical pesticides seriously threatens the environment and food safety, so that the urgent requirements for solving the current environmental pollution and food safety crisis are to find a novel plant protection method, a novel technology and a novel product development which are efficient and have good compatibility with the environment.
In the existing novel fertilizer technology, the main stream technology is the mixing of pesticide and fertilizer, the pesticide and fertilizer is mainly mixed when being used in the existing preparation, the problems of long-term storage, deterioration and denaturation or generation of more difficult-to-degrade harmful substances cannot be solved, the same effect of pesticide and fertilizer or high efficiency of pesticide and fertilizer cannot be accurately realized, and the requirements of green and environmental protection cannot be met; secondly, a microorganism addition method is adopted, but the method has important defects in technology, the problem of long-term survival of microorganisms cannot be solved under the condition of high concentration of chemical nutrients, and the specific effect of the microorganisms cannot be realized; and nutrient substances such as humic acid, amino acid, molasses, yeast waste liquid, plant growth regulator, macroelements, medium trace elements and the like are simply mixed, dissolved or chelated to prepare the liquid or solid fertilizer. Due to the disordered market, simple technical process, various product qualities, single fertilizer effect and function, low nutrient content and poor plant absorption and utilization rate. Many water-soluble fertilizer products abuse chemical hormones, plant growth regulators, auxiliary agents and the like, so that the quality of agricultural products is reduced, the safety risk is increased, and the development requirements of future high-efficiency agriculture and green ecological agriculture cannot be met at all.
Disclosure of Invention
The invention aims to provide a novel multifunctional liquid fertilizer which is prepared from macroelements, medium and trace elements, EDTA (ethylene diamine tetraacetic acid), single-cell protein microcapsules, nano-silver composite liquid, nano-titanium-carbon composite liquid, composite active polypeptide protease liquid and water, has balanced nutrition and high absorption and utilization rate, prevents and treats plant diseases and insect pests, enhances plant immunity, increases yield, improves quality and improves soil, and a preparation method thereof.
The technical scheme adopted by the invention for solving the technical problems is as follows: an environment-friendly multifunctional liquid fertilizer is prepared from the following components in parts by weight: 700 parts of macroelement 400-; the nano-silver composite liquid is prepared from nano-silver colloidal particles, sodium citrate, glucose and deionized water, the nano-titanium-carbon composite liquid is prepared from nano-titanium, nano-biochar, phosphoric acid and water, the composite active polypeptide protease liquid is prepared from active polypeptide protease, sugar alcohol, hydroxypropyl chitosan particles, acetic acid and deionized water, and the single-cell protein microcapsule is prepared from yeast protein, bacterial protein, algal protein and biological polymer microspheres.
Preferably, the environment-friendly multifunctional liquid fertilizer is prepared from the following components in parts by weight: 600 parts of macroelement, 80 parts of medium trace element, 15 parts of EDTA, 0.5 part of nano-silver composite liquid, 5 parts of nano-titanium-carbon composite liquid, 10 parts of composite active polypeptide protease liquid, 10 parts of single-cell protein microcapsule and 279.5 parts of water.
The macroelement is preferably prepared by mixing urea, dipotassium hydrogen phosphate, diammonium hydrogen phosphate and potassium dihydrogen phosphate according to the weight ratio of 0.5-1.5:2-3:0.01-0.05: 0.1-1.5.
The medium trace elements are preferably prepared by mixing ferrous sulfate, copper sulfate, magnesium sulfate, zinc sulfate, manganese sulfate, boric acid, ammonium molybdate and calcium nitrate according to the weight ratio of 0.5-1:0.5-1:0.5-1:0.5-1: 0.5-1.
The compound active polypeptide protease liquid is preferably prepared by condensation reaction of active polypeptide protease, sugar alcohol, hydroxypropyl chitosan particles, acetic acid and deionized water according to the weight ratio of 1-1.5:1-1.5:1-1.5:1-1.5: 1-2; the active polypeptide protease is prepared by performing hydrolysis reaction on antibacterial peptide in bioactive peptide, protease extracted from plants, glycerol, water and sodium bisulfite in a reaction kettle according to the weight ratio of 1:1:1:2: 0.05.
The nano-silver composite liquid is preferably prepared by carrying out complex reaction on nano-silver colloidal particles, sodium citrate, glucose and deionized water according to the weight ratio of 1-2:1-1.5:1-1.5: 1.5-2.
The nano titanium-carbon composite liquid is preferably prepared by carrying out complex reaction on nano titanium, nano biochar, phosphoric acid and water according to the weight ratio of 1-1.5:1-1.5:1-1.5: 1-2.
The single-cell protein microcapsule is preferably formed by polymerizing yeast protein, bacterial protein and algae protein with biopolymer microspheres according to the weight ratio of 1-2:1-1.5: 0.5-1.5; wherein the weight ratio of the total amount of the yeast protein, the bacterial protein and the algae protein to the biopolymer microspheres is 2-3: 1-2.
The antibacterial peptide, the protease extracted from the plant, the glycerol, the water and the sodium bisulfite according to the technical scheme of the invention can be directly purchased in the market, such as the antibacterial peptide of Beijing kang peptide Biotech Co., Ltd, the plant protease of Hebei Linkang Biotech Co., Ltd, Beijing Shengshi Jiaming science and technology development Co., Ltd and the like.
The yeast protein, bacterial protein, algae protein and biopolymer microspheres described in the technical scheme of the invention can be directly purchased in the market, such as yeast protein powder of Angel yeast, bacterial protein of Wuhan Dahuawei pharmaceutical and chemical (group) company, algae protein of Shandong Linyifeng plant Hospital, Inc., Shandong Zhongzhou jin sun Biotech, Inc., biopolymer microspheres of Beijing Shengdong Tech, Wuhan Guangming Chuangbo bioproduct, Inc.
Furthermore, the hydroxypropyl chitosan particles of the invention preferably adopt hydroxypropyl chitosan particles with the particle size of 10-30 nm.
The invention also provides a method for preparing the environment-friendly multifunctional liquid fertilizer, which sequentially comprises the following steps:
(1) crushing and metering raw materials: metering the components according to the weight ratio; respectively inputting macroelements and medium-trace elements into two different proportioning bins;
(2) inputting nano silver colloidal particles, sodium citrate, glucose and deionized water into a reaction kettle according to a ratio for carrying out a complex reaction to prepare nano silver composite liquid;
(3) inputting nano titanium, nano biochar, phosphoric acid and water into a reaction kettle according to a ratio for a complex reaction to prepare nano titanium-carbon composite liquid;
(4) inputting active polypeptide protease, sugar alcohol, hydroxypropyl chitosan particles, acetic acid and deionized water into a condensation polycondensation reaction system according to a ratio to prepare a composite active polypeptide protease liquid;
(5) inputting the yeast protein, the bacterial protein and the algae protein and the biopolymer microspheres into a condensation polycondensation reaction system according to the proportion for polymerization reaction to prepare single-cell protein microcapsules;
(6) injecting water into the high-shear reaction kettle, introducing steam for heating, and heating to 60-70 ℃;
(7) injecting the macroelements in the batching bin into a high-shear reaction kettle for stirring, and dissolving the macroelements;
(8) after the macroelements are completely dissolved, injecting the medium trace elements in the other batching bin into the high-shear reaction kettle; continuously heating to 80-90 ℃ and accelerating stirring;
(9) pressing EDTA into the high-shear reaction kettle to chelate the medium and trace elements;
(10) stirring, cooling to 60-65 deg.C, and injecting the nano silver composite solution and the nano titanium-carbon composite solution;
(11) continuously stirring, cooling to 45-55 deg.C, and pressing in composite plant active polypeptide protease liquid and single cell protein microcapsule; keeping the temperature at 40 ℃ and stirring for 2 hours;
(12) adjusting the pH value to 5.5-6.5;
(13) continuously cooling to below 35 ℃;
(14) sampling and detecting;
(15) testing the stability;
(16) metering and packaging;
(17) and (6) warehousing the finished product.
The environment-friendly multifunctional liquid fertilizer disclosed by the invention is illustrated by the following raw material formula and technical principle: in the liquid fertilizer, macroelements and medium-trace elements are nutrients basically required by plants, and besides the preferable components and proportion, the macroelements can also be selected from urea, dipotassium hydrogen phosphate, potassium chloride, potassium sulfate, monopotassium phosphate, potassium nitrate, ammonium sulfate, diammonium hydrogen phosphate, monoammonium phosphate, potassium phosphate, ammonium polyphosphate, calcium ammonium nitrate and the like which can provide the components basically required by the nutrients for the plants; the medium trace elements can be ferrous sulfate, copper nitrate, magnesium sulfate, zinc sulfate, manganese sulfate, boric acid, ammonium molybdate, calcium nitrate and other components which can provide basic nutrient requirements for plants.
The nano silver particles directly enter the thallus to be combined with oxygen metabolizing enzyme (-SH), so that the thallus is choked to death by a unique action mechanism, and most of microbes such as bacteria, fungi, mold, spores and the like which are contacted with the thallus can be killed. Experimental research shows that Ag can kill more than 650 bacteria within minutes, and after the nano silver particles are combined with cell walls/membranes of pathogenic bacteria, the nano silver particles can directly enter thalli and are rapidly combined with sulfydryl (-SH) of oxygen metabolism enzyme, so that the enzyme is inactivated, respiratory metabolism is blocked, and the bacteria are suffocated. The unique sterilization mechanism enables the nano silver particles to rapidly kill pathogenic bacteria at low concentration. So far, nano silver has been widely used in medicine and products such as baby feeding bottles, toys, toothbrushes, toothpaste, air purifiers, etc. because of its excellent antibacterial effect; in the fields of environment and agriculture, the bactericide is mainly used as a crop bactericide, a fruit and vegetable bactericide and a water body bactericide.
The nano titanium-carbon composite liquid is mainly prepared from nano titanium and nano biochar in proportion, has the functions of photocatalyst sterilization and photosynthesis promotion, and has an effect similar to hormone (auxin and cytokinin) on the growth of plants by the nano titanium element. The nano titanium-carbon composite liquid can promote the rapid growth of plants; the disease resistance, waterlogging resistance, drought resistance, cold resistance and aging resistance of the plants are improved; increasing the yield, improving the quality of crops and the like, and is used for the fresh-keeping, the disinfection and the sterilization, the non-test-tube rapid propagation of plants, the seed treatment of plants and the like of nanometer silk flowers, nanometer biological pesticides, saline-alkali soil improvement and fruits and vegetables in agricultural production.
The active polypeptide protease is prepared by performing hydrolysis reaction on antibacterial peptide in bioactive peptide, protease extracted from plants, glycerol, water and sodium bisulfite in a reaction kettle according to the weight ratio of 1:1:1:2: 0.05. The antibacterial peptide antigen refers to a basic polypeptide substance with antibacterial activity generated by induction in an insect body, the molecular weight is about 2000-7000, the basic polypeptide substance is composed of 20-60 amino acid residues, and most of the active polypeptides have the characteristics of strong basicity, thermal stability, broad-spectrum antibacterial property and the like. The first discovered antibacterial peptide in the world is a polypeptide with antibacterial activity produced by Swedish scientist G.Boman et al by injecting Neisseria monocytogenes and Escherichia coli to induce Xigubia silkworm pupae in 1980, and is named as Cecropins. Originally, when studying the immune mechanism of the north american wild silkworm, people found that after the diapause pupae are induced by external stimulation, polypeptide substances with bacteriostatic action are generated in the haemolymph, and the antibacterial polypeptide is named Cecropins (Cecropins). Subsequently, antibacterial polypeptides having similar structures were also isolated from other insects, amphibians and mammals, and the structures of more than 70 antibacterial polypeptides were determined. Polypeptides having antibacterial activity were discovered and isolated from bacteria, fungi, amphibians, insects, higher plants, mammals, and even humans in succession during the years after 1980. Because the active polypeptide has broad-spectrum efficient bactericidal activity on bacteria, the active polypeptide is named as 'antibacterial peptides, ABP', and is translated into antibacterial peptide which is originally intended to be the antibacterial peptide. Antibacterial peptides have potent killing effects on some fungi, protozoa, viruses, cancer cells, etc., and thus many scholars who name such active polypeptides tend to refer to them as "peptide antibiotics. The antibacterial peptide is applied to agriculture, and is mainly used for transforming crops to culture disease-resistant varieties. As the antibacterial peptide has bactericidal activity to various plant pathogenic bacteria, the anti-bacterial gene is introduced into the human body to express in the plant body, so that the disease resistance of the plant can be improved. The antimicrobial peptide industry is an emerging high-tech biotechnology and biotechnology industry. Because of the imperfect early bioengineering methods, extraction of antimicrobial peptides is extremely expensive, limiting their application in medicine, agriculture, and industry. With the further development of transgenic technology, the mass production of antibacterial peptides can be carried out by using engineered bacteria or yeast.
The plant protease is a product formed by taking plants as raw materials, directionally obtaining and concentrating one or more effective components in the plants through a physical and chemical extraction and separation process according to the requirements of the application of the extracted product without changing the structures of the effective components; it is widely used in the fields of health food, cosmetics, medicine, daily necessities, agriculture and the like. The raw materials extracted by the plant protease are wide in range, such as soybeans, green beans, broad beans, kidney beans, peas, small red beans, mung beans, peanuts, small barley, corns, buckwheat, wheat, mushrooms, tremella, agaric and the like. The prepared active polypeptide protease has obvious effects on the aspects of physiological regulation of crops, cell tissue activation, plant stress resistance improvement, crop disease index reduction and the like, plant vitality improvement and crop quality improvement; can promote the absorption of crops to nutrients and improve the utilization rate of fertilizers. The active polypeptide protease has the functions of plant cell immune factors, can induce plant resistance, repair and strengthen a plant immune function system, improve the plant stress resistance, promote the positive energy ecological growth of plants, and improve the plant self resistance, the immune ability and the disease resistance.
The single-cell protein microcapsule of the invention is composed of various proteases of microorganisms and biological polymer microspheres, fixes the single-cell proteases on the microspheres, and is the optimal combination of physical adsorption and chemical combination. The enzyme immobilized on the functional microsphere has high pH stability, thermal stability and storage stability, is easy to separate from reactants, has good biodegradability, and can promote multi-enzyme reaction by the multi-enzyme combined immobilized microsphere. The single-cell protein combined with microsphere is also called microbial protein, and is a product obtained by using microbial thallus artificially cultured from many industrial and agricultural wastes and petroleum wastes through treatment. The single-cell protein is not a pure protein, but is a cytoplasm cluster consisting of a mixture of protein, fat, carbohydrate, nucleic acid, nitrogen-containing compounds other than protein, vitamins, inorganic compounds and the like, and also contains various vitamins, carbohydrates, lipids, minerals, trace elements, rich enzymes and bioactive substances, such as coenzyme A, coenzyme Q, glutathione, ergosterol and the like, which can be directly absorbed and utilized by plants to provide various nutrient substances for the plants. The unicellular protein is produced by microorganism, has biological activity to various crops, belongs to protein excitant class drugs, has no direct killing effect to pathogens unlike biological pesticides in general concepts, enhances the immunocompetence of plants and promotes the growth of plants by exciting the expression of disease-resistant and insect-resistant growth and development related genes of the plants. Is safe and nontoxic, does not cause plant resistance, has obvious effects of resisting diseases and increasing yield for plants, and can improve the quality of crops.
Compared with the prior art, the invention has the beneficial effects that: the environment-friendly multifunctional liquid fertilizer obtained by adopting the technical scheme of the invention has the comprehensive functions of balancing nutrition, preventing and treating plant diseases and insect pests, enhancing plant immunity, increasing yield, improving quality and improving soil; the technology and the product have wide application range, can reduce the dosage of chemical fertilizers and toxic and harmful pesticides, realize the integration of pesticide and fertilizer and the integration of water and fertilizer, and are safe, efficient, green and environment-friendly.
Detailed Description
The present invention is further illustrated in detail by the following examples, which are provided only for illustrating the present invention and are not intended to limit the scope of the present invention.
Example 1 the invention was carried out by the following steps, preparing an environment-friendly multifunctional liquid fertilizer:
1. preparing the following components in parts by weight: 600Kg of macroelement, 80Kg of medium trace element, 15Kg of EDTA, 0.5Kg of nano-silver composite solution, 5Kg of nano-titanium-carbon composite solution, 10Kg of composite active polypeptide protease solution, 10Kg of single-cell protein microcapsule and 279.50Kg of water; wherein,
firstly, mixing 100Kg of urea, 490Kg of dipotassium hydrogen phosphate, 5Kg of diammonium hydrogen phosphate and 5Kg of monopotassium phosphate to prepare a major element;
② the trace elements are prepared by mixing 10Kg of ferrous sulfate, 10Kg of copper sulfate, 10Kg of magnesium sulfate, 10Kg of zinc sulfate, 10Kg of manganese sulfate, 10Kg of boric acid, 10Kg of ammonium molybdate and 10Kg of calcium nitrate;
③ the nano-silver complex liquid is prepared by the complex reaction of 0.125Kg of nano-silver colloidal particle, 0.125Kg of sodium citrate, 0.125Kg of glucose and 0.125Kg of deionized water;
fourthly, the nano titanium-carbon composite solution is prepared by the complex reaction of 1.25Kg of nano titanium, 1.25Kg of nano biochar, 1.25Kg of phosphoric acid and 1.25Kg of water;
the composite active polypeptide protease liquid comprises the following components: 2Kg of active polypeptide protease, 2Kg of sugar alcohol, 2Kg of hydroxypropyl chitosan particles, 2Kg of acetic acid and 2Kg of deionized water; wherein the active polypeptide protease is prepared by the hydrolysis reaction of antibacterial peptide (purchased from Beijing kang peptide Biotech Co., Ltd.), protease extracted from plants (purchased from Hebeilin Linkang Biotech Co., Ltd.), glycerol, water and sodium bisulfite in a reaction kettle according to the weight ratio of 1:1:1:2: 0.05;
the unicellular protein microcapsule is polymerized by 1Kg of yeast protein, 1Kg of bacterial protein, 1Kg of algae protein and 1Kg of biopolymer microspheres, the yeast protein is Angel yeast protein powder, the bacterial protein is purchased from great warburg pharmaceutical chemical (group) of Wuhan, the algae protein is purchased from Binzhou Sungtai Biotech Limited, Shandong province, and the biopolymer microspheres are purchased from Beijing Shengdong Tech Co Ltd.
The preparation method of the environment-friendly multifunctional liquid fertilizer sequentially comprises the following steps:
(1) crushing and metering raw materials: mixing macroelements and medium-trace elements in proportion and inputting the mixture into different proportioning bins;
(2) inputting nano silver colloidal particles, sodium citrate, glucose and deionized water into a reaction kettle for complex reaction to prepare nano silver composite liquid;
(3) inputting nano titanium, nano biochar, phosphoric acid and water into a reaction kettle for complex reaction to prepare nano titanium-carbon composite liquid;
(4) inputting active polypeptide protease, sugar alcohol, hydroxypropyl chitosan particles, acetic acid and deionized water into a condensation polycondensation reaction system to prepare a composite active polypeptide protease liquid;
(5) inputting the yeast protein, the bacterial protein and the algae protein into a condensation polycondensation reaction system according to the proportion for polymerization reaction to prepare single-cell protein microcapsules;
(6) injecting water into the high-shear reaction kettle, introducing steam for heating, and heating to 60-70 ℃;
(7) injecting the macroelements in the batching bin into a high-shear reaction kettle, and stirring to dissolve the macroelements;
(8) after the macroelements are completely dissolved, injecting the microelements in the batching bin into the high-shear reaction kettle; continuously heating to 80-90 ℃ and accelerating stirring;
(9) pressing EDTA into the high-shear reaction kettle to chelate trace elements;
(10) stirring and cooling to 60-65 deg.C, and injecting the nano silver composite liquid and nano titanium-carbon composite liquid;
(11) continuously stirring and cooling to 45-55 deg.C, and pressing in the composite plant active polypeptide protease liquid and single-cell protein microcapsule; keeping stirring for 2 hours at the constant temperature of 40 ℃;
(12) adjusting the pH value to 5.5-6.5;
(13) continuously cooling to below 35 ℃;
(14) sampling and detecting;
(15) testing the stability;
(16) metering and packaging;
(17) and (6) warehousing the finished product.
Example 2 an environment-friendly multifunctional liquid fertilizer was prepared from the following components in parts by weight: 550Kg of macroelement, 90Kg of medium trace element, 20 Kg of EDTA, 0.8 Kg of nano-silver composite solution, 8 Kg of nano-titanium-carbon composite solution, 15Kg of composite active polypeptide protease solution, 15Kg of single-cell protein microcapsule and 301.2 Kg of water. The preparation method is the same as example 1.
Example 3 an environment-friendly multifunctional liquid fertilizer was prepared from the following components in parts by weight: 550Kg of macroelement, 100Kg of medium trace element, 25Kg of EDTA, 1Kg of nano-silver composite solution, 10Kg of nano-titanium-carbon composite solution, 10Kg of composite active polypeptide protease solution, 10Kg of single-cell protein microcapsule and 294 Kg of water. The preparation method is the same as example 1.
Example 4 application test of the environment-friendly multifunctional liquid fertilizer of the present invention.
First, field test application on tomato
1. Materials and methods
1.1 test crop and target: the crop is a tomato 'Nongbo red crown' variety, and aims at promoting the growth and development of tomatoes and preventing and treating tomato epidemic diseases
1.2 basic conditions of the test: the test site is arranged in a tomato planting base in the Dongyi countryside of the city of Lu Yi in Shanxi province, the soil texture is carbonate brown soil, the organic matter content is 19.2g/Kg, the total nitrogen content is 1.63g/Kg, the available phosphorus content is 20.2 mg/Kg, and the available potassium content is 180.5 mg/Kg. The tomato variety to be tested is planted in the near brick-concrete structure greenhouse in the way of matrix plug seedling cultivation in 2016, 3 months and 24 days, the seedling period is about 40 days, and the tomato variety begins to be planted in the open field before 5 months and 4 days. The demonstration test field is mulching film coverage, the row spacing is 0.5m, the average row spacing is 0.65 m, and the density is about 2050 plants/667 square meters. No soil sanitizer was used. The cultivation conditions, the crop varieties and the fertilizer and water management conditions of each experimental plot in the field are consistent, and the method completely accords with local production practice.
2. Design and arrangement of experiments
2.1 products of use
A: the environment-friendly multifunctional liquid fertilizer comprises the following components: n + P2O5+K2O is more than or equal to 500g/L, Cu, Mn + Zn + B + Mo + Fe + Mg + Ca is more than or equal to 8g/L, nano-silver is 0.5g/L, nano-titanium-carbon composite liquid is 5g/L, composite active polypeptide protease is 10g/L, single-cell protein microcapsule is 10g/L, and the product form is liquid.
B: the functional water-soluble fertilizer special for Icelv Volski (FOFS LTD) Wo leaf brand tomatoes for preventing and treating the tomato epidemic disease contains macroelements, medium and trace elements and rare earth multi-nutrient.
2.2 cell design arrangement: the field random block is arranged, and two treatments are respectively carried out: the treatment A is spraying of an environment-friendly multifunctional liquid fertilizer diluted by 800 times; the treatment B is an imported functional water-soluble fertilizer for preventing and treating tomato epidemic disease by Israeli Volski company (FOFS LTD) Wo-leaf brand tomatoes, and contains macroelements, medium and trace elements and rare earth multi-nutrient; control (CK) clear water. Each treatment cell had an area of 740 square meters, 11 membrane rows of 22 and about 2280 plants were one cell, 3 replicates were used, 9 total cells were used, and the test field was 10 acres.
2.3 modes of application
2.3.1 application time and frequency: the application time is arranged in the whole tomato growth period and is respectively 4% in afternoon at 7 months 1, 7 months 8, 7 months 15, 7 months 25, 8 months 5 and 8 months 15: 30 spraying is carried out. The dosage and the use time are as follows:
2.3.2 use of the device and method of application: in order to ensure the application quality and effect, the front and back surfaces of the tomato leaf are evenly coated with liquid, and the average dosage of the spraying per cell is 50-80 kg of aqueous solution.
2.4 meteorological data: during the implementation, the weather is basically normal and rainless, and no influence is caused on the sunny day after spraying. During six spraying periods, the local air temperature is maintained at a normal level, the average air temperature is 23 ℃, the maximum air temperature is 31 ℃, the minimum air temperature is 18 ℃, and the influence of other severe weather factors is avoided.
3. Investigation of the field
3.1 survey and grading criteria: the incidence and disease index were investigated for the first time before administration, after which the investigation was carried out 3 days after administration. Sampling is carried out at five random points in each cell, 3 tomatoes are investigated at each point for 15 plants in total, 10 leaves are investigated from top to bottom according to the disease incidence condition of each plant (the same leaf position is determined from bottom to top in each investigation), the area of the disease spots on each leaf accounts for the whole leaf area to be graded, and then the total leaf number, the disease leaf number and the disease severity are recorded. Finally, the disease leaf rate, disease index and prevention and treatment effect are calculated. Grading the disease condition standard: the unit of leaf is taken, and the degree of disease is taken as the grade
Grade 0 non-speckled
The area of the grade 1 lesion spots accounts for less than 5% of the whole leaf area;
the area of the 3-grade scab accounts for 6-10% of the whole leaf area;
the area of the 5-grade scab accounts for 11% -25% of the area of the whole leaf;
the 7-grade lesion area accounts for 26-50% of the whole leaf area;
the area of the 9-grade lesion spots accounts for more than 51 percent of the whole leaf area.
3.2 investigation time: and (4) collecting leaves in the field according to a five-point sampling method six days after each application, and counting and recording the disease occurrence condition according to a grading standard. The investigation time was 7 months and 25 days, and 8 months and 16 days, respectively.
3.3 survey data and calculation method: the survey data and the calculation results of 3 applications and 3 replicates of each treatment are detailed in table three. The disease leaf rate, disease index and prevention and treatment effect are calculated according to the following formulas, and statistical analysis is carried out on test data by a Duncan's New Duck error (DMRT) method.
3.4 effects on crops: during the test period, the multifunctional liquid fertilizer has no occurrence of phytotoxicity to tomatoes in various treatment concentrations.
3.5 Effect on other organisms: the beneficial or harmful effects of the multifunctional liquid fertilizer on organisms other than the target are not found during the test.
4. Test results and analysis
4.1 the effect and result statistics of the application of the environment-friendly multifunctional liquid fertilizer to tomato seedlings are shown in table 1.
From table 1, it can be seen that the survival rate and uniformity of tomato seedlings can be significantly improved by applying the environment-friendly multifunctional liquid fertilizer 800X of the invention, and the comprehensive properties of the tomato seedlings are greatly improved.
4.2 the influence of the multifunctional liquid fertilizer on the disease resistance and the growth potential of the tomatoes is shown in the following table.
From table 2, it can be seen that the multifunctional liquid fertilizer of the present invention has the function of enhancing plant epidemic disease resistance, and simultaneously, the plant leaves are dark green after the multifunctional liquid fertilizer is used, and the multifunctional liquid fertilizer has the function of enhancing photosynthesis, thereby being beneficial to coloring tomato fruits and improving the quality.
4.3 the control effect of the environment-friendly multifunctional liquid fertilizer on the tomato epidemic disease is compared with the promotion effect on the plant growth: the control effect on tomato blight is shown in table 3, and the control effect on tomato late blight and the promotion effect on plant growth after the environment-friendly multifunctional liquid fertilizer is applied are shown in table 4.
From tables 3 and 4, it can be seen that the control effect of the tomato epidemic disease after the treatment (applying the environment-friendly multifunctional liquid fertilizer of the invention) is 87.5%, which is 12.3% higher than the control effect of the control agent B (i.e. the functional water-soluble fertilizer for controlling tomato epidemic disease special for Fos-leaf-brandy tomatoes, by Fofs LTD), and the difference is significant. The incidence of the treatment A is 18 percent lower than that of the control, the difference is obvious, and the difference is 4.8 percent lower than that of the treatment B, and the difference is not obvious. Meanwhile, the control effect of the treatment A is good, so that the tomato leaves are kept well, the color of the leaves is dark green, the growth period is prolonged to 11 days, the harvesting period is prolonged, and the yield is increased. It can also be seen from table 3 that the exemplary test field tomato blight occurred universally and evenly; the investigation result 3d after the last spraying can also be seen, the disease indexes in the later period are all increased, the average disease index in the blank control area is increased from 4.1 to 11.9, the increase is particularly obvious, and the disease development in the test field is rapid and serious.
4.4 Effect on tomato yield after Combined application: the cell throughput measurements and the results of the investigation and analysis are shown in table 5.
It can be seen from table 5 that the yield of the plot is significantly increased after the environmental-friendly multifunctional liquid fertilizer of the present invention is applied, the yield is 10.2 percentage points higher than that of the control B (i.e. the water-soluble fertilizer for controlling tomato epidemic disease with the special functionality of Wobbe tomato by Islefluviatilis company (FOFS LTD)), and 17.3 percentage points higher than that of the blank control area, and the difference is significant. The yield of the control B treated area was also 7.1 percentage points higher than the control area, with a significant difference.
5. Test conclusions and analytical evaluation
5.1 conclusion of the test
In conclusion, after the environment-friendly multifunctional liquid fertilizer is applied, the tomato has light disease occurrence, dark green leaves, strong growth, prolonged growth period and harvesting period, good fruit coloring, obviously improved quality and yield and obvious control effect.
5.2 evaluation
The test result shows that the tomato late blight disease prevention effect is more than 80% after the tomato late blight disease prevention agent is sprayed every 10 days after the tomato late blight disease prevention agent is sprayed, and the development harm of the tomato late blight disease is effectively controlled.
Second, test application on watermelon
1. Basic information of experiment
1.1 purpose of the test: the practical effect of the environment-friendly multifunctional liquid fertilizer on the watermelon is verified.
1.2 test fertilizers: the environment-friendly multifunctional liquid fertilizer comprises the following components: n + P2O5+K2O is more than or equal to 500g/L, Cu, Mn + Zn + B + Mo + Fe + Mg + Ca is more than or equal to 8g/L, nano-silver is 0.5g/L, nano-titanium-carbon composite liquid is 5g/L, composite active polypeptide protease is 10g/L, single-cell protein microcapsule is 10g/L, and the product form is liquid.
1.3 test time: 15 days 3 and 2016 to 15 days 6 and 2016
2. Materials and methods
2.1 test site: the area of a test field is 10 mu in a indocalamus transverse village watermelon planting base in Wenling mountain of Zhejiang province.
2.2 basic properties of the tested soil: the soil type is the matrix of the mature soil: loess of Sichuan system, retention type paddy soil, and horse liver soil. The basic physical and chemical properties of the fertilizer after autumn sampling and testing in 2015 are 18.83g/kg of soil organic matter, 1.42g/kg of total nitrogen, 26.5mg/kg of available phosphorus, 85.5mg/kg of quick-acting potassium and pH6.8.
2.3 test crop name and variety: planting watermelon as crop, the variety is Xiaolan. Variety characteristics: the whole growth period of the small orchid watermelon is 90-120 days, the flower skin is yellow, the small orchid watermelon is extremely early-maturing, low-temperature and weak-light resistant, the bearing capacity is strong, and the weight of a single fruit is 1.5-2.5 kg.
2.4 test treatment: the experiment was set to 3 treatments:
① conventional Control (CK)1): base ofAnd performing topdressing according to the local conventional use condition. No leaf fertilizer and regulator are used;
② clear water Control (CK)2): spraying 3 equal amount of clear water and the like per mu each time, and treating 1 in the same way by other fertilization measures;
③ the environmental protection type multifunctional liquid fertilizer of the invention is applied with water after watermelon vine extraction, at fruit setting stage, fruit swelling stage and mature stage each time 5kg of the environmental protection type multifunctional liquid fertilizer provided by the invention is applied with water, the whole growth cycle is applied 4 times, other fertilizing measures are treated similarly 1. the concrete cultivation measures are as follows, 5kg of the environmental protection type multifunctional liquid fertilizer is applied at the vine extending stage (4 months and 2 days), 5kg of the environmental protection type multifunctional liquid fertilizer is applied at the fruit setting stage (4 months and 20 days), 5kg of the environmental protection type multifunctional liquid fertilizer is applied at the fruit swelling stage (5 months and 3 days), 5kg of the environmental protection type multifunctional liquid fertilizer is applied at the mature stage (5 months and 10 days), 3 times of the test are repeated, random block design is arranged, the block is arranged along the direction of soil fertility change, one block is arranged in one field, and the area is basically uniform and 20m in area2The length-width ratio is 4 m × 5m, and the cells are isolated by protection lines.
Fourthly, the variety and the quantity of the topdressing. Base fertilizer: applying 2000 kg of decomposed duck manure, 150 kg of rapeseed cakes and 20 kg of 45% potassium sulfate compound fertilizer to each mu; topdressing: in the expansion period of watermelons, 10 kilograms of 45 percent potassium sulfate compound fertilizer is applied per mu.
2.5 demonstration procedure
(1) Sowing and seedling raising period: 6 days in 1 month, 15 days in transplanting period and 25 days in extending period
(2) Fruit setting stage: 16 days at 4 months, 30 days at 4 months at the watermelon expansion period, and a harvesting period: 20 days in 5 months, 15 days in 6 months
(3) Disease and pest control: the pesticide is mainly used for preventing anthracnose in seedling stage, 1000 times of 70% thiophanate methyl is sprayed, aphids, red spiders and the like are prevented in spreading stage, and 10% imidacloprid 40 g is sprayed per mu by adding 20 kg of water.
3. Results and analysis
3.1 Effect of different treatments on watermelon phytology
3.2 Effect of different treatments on yield
3.3 statistical analysis of yields
Treatment 3 differed from treatment 2 and treatment 1 in significance and extreme significance as tested by multiple comparisons of mean values.
4. And (4) test conclusion: the following results are obtained by experiments and data analysis: the environment-friendly multifunctional liquid fertilizer has a strong yield-increasing effect on the production of watermelon fields. The yield is increased by 54.92 percent compared with clear water, 54.5 percent compared with the conventional control, and the mouthfeel is good.
Claims (10)
1. The environment-friendly multifunctional liquid fertilizer is characterized in that: the composition is prepared from the following components in parts by weight: 700 parts of macroelement 400-; the nano-silver composite liquid is prepared from nano-silver colloidal particles, sodium citrate, glucose and deionized water, the nano-titanium-carbon composite liquid is prepared from nano-titanium, nano-biochar, phosphoric acid and water, the composite active polypeptide protease liquid is prepared from active polypeptide protease, sugar alcohol, hydroxypropyl chitosan particles, acetic acid and deionized water, and the single-cell protein microcapsule is prepared from yeast protein, bacterial protein, algal protein and biological polymer microspheres.
2. The environment-friendly multifunctional liquid fertilizer according to claim 1, wherein: the composition is prepared from the following components in parts by weight: 600 parts of macroelements, 80 parts of medium trace elements, 15 parts of EDTA (ethylene diamine tetraacetic acid), 0.5 part of nano-silver composite liquid, 5 parts of nano-titanium-carbon composite liquid, 10 parts of composite active polypeptide protease liquid, 10 parts of single-cell protein microcapsules and 279.5 parts of water.
3. The environment-friendly multifunctional liquid fertilizer according to claim 1 or 2, wherein: the macroelements are a mixture prepared from urea, dipotassium hydrogen phosphate, diammonium hydrogen phosphate and potassium dihydrogen phosphate according to the weight ratio of 0.5-1.5:2-3:0.01-0.05: 0.1-1.5.
4. The environment-friendly multifunctional liquid fertilizer according to claim 1 or 2, wherein: the medium trace elements are a mixture prepared from ferrous sulfate, copper sulfate, magnesium sulfate, zinc sulfate, manganese sulfate, boric acid, ammonium molybdate and calcium nitrate according to the weight ratio of 0.5-1:0.5-1:0.5-1:0.5-1: 0.5-1.
5. The environment-friendly multifunctional liquid fertilizer according to claim 1 or 2, wherein: the nano-silver composite liquid is prepared by carrying out complex reaction on nano-silver colloidal particles, sodium citrate, glucose and deionized water according to the weight ratio of 1-2:1-1.5:1-1.5: 1.5-2.
6. The environment-friendly multifunctional liquid fertilizer according to claim 1 or 2, wherein: the nano titanium-carbon composite liquid is prepared by carrying out complex reaction on nano titanium, nano biochar, phosphoric acid and water according to the weight ratio of 1-1.5:1-1.5:1-1.5: 1-2.
7. The environment-friendly multifunctional liquid fertilizer according to claim 1 or 2, wherein: the compound active polypeptide protease liquid is prepared by condensation reaction of active polypeptide protease, sugar alcohol, hydroxypropyl chitosan particles, acetic acid and deionized water according to the weight ratio of 1-1.5:1-1.5:1-1.5:1-1.5: 1-2.
8. The environment-friendly multifunctional liquid fertilizer according to claim 7, wherein: the active polypeptide protease is prepared by hydrolysis reaction of antibacterial peptide in bioactive peptide, protease extracted from plants, glycerol, water and sodium bisulfite in a reaction kettle according to the weight ratio of 1:1:1:2: 0.05.
9. The environment-friendly multifunctional liquid fertilizer according to claim 1 or 2, wherein: the single-cell protein microcapsule is polymerized by yeast protein, bacterial protein and algae protein according to the weight ratio of 1-2:1-1.5:0.5-1.5 and biological polymer microspheres; the weight ratio of the total amount of the yeast protein, the bacterial protein and the algae protein to the biopolymer microspheres is 2-3: 1-2.
10. The environment-friendly multifunctional liquid fertilizer according to claim 1, wherein: the preparation method comprises the following steps:
(1) crushing and metering raw materials: metering the components according to the weight ratio; respectively inputting macroelements and medium-trace elements into two different proportioning bins;
(2) inputting nano silver colloidal particles, sodium citrate, glucose and deionized water into a reaction kettle according to a ratio for carrying out a complex reaction to prepare nano silver composite liquid;
(3) inputting nano titanium, nano biochar, phosphoric acid and water into a reaction kettle according to a ratio for a complex reaction to prepare nano titanium-carbon composite liquid;
(4) inputting active polypeptide protease, sugar alcohol, hydroxypropyl chitosan particles, acetic acid and deionized water into a condensation polycondensation reaction system according to a ratio to prepare a composite active polypeptide protease liquid;
(5) inputting the yeast protein, the bacterial protein and the algae protein and the biopolymer microspheres into a condensation polycondensation reaction system according to the proportion for polymerization reaction to prepare single-cell protein microcapsules;
(6) injecting water into the high-shear reaction kettle, introducing steam for heating, and heating to 60-70 ℃;
(7) injecting the macroelements in the batching bin into a high-shear reaction kettle for stirring, and dissolving the macroelements;
(8) after the macroelements are completely dissolved, injecting the medium trace elements in the other batching bin into the high-shear reaction kettle; continuously heating to 80-90 ℃ and accelerating stirring;
(9) pressing EDTA into the high-shear reaction kettle to chelate the medium and trace elements;
(10) stirring, cooling to 60-65 deg.C, and injecting the nano silver composite solution and the nano titanium-carbon composite solution;
(11) continuously stirring, cooling to 45-55 deg.C, and pressing in composite plant active polypeptide protease liquid and single cell protein microcapsule; keeping the temperature at 40 ℃ and stirring for 2 hours;
(12) adjusting the pH value to 5.5-6.5;
(13) continuously cooling to below 35 ℃;
(14) sampling and detecting;
(15) testing the stability;
(16) metering and packaging;
(17) and (6) warehousing the finished product.
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| CN113880641A (en) * | 2021-11-02 | 2022-01-04 | 台沃科技集团股份有限公司 | Preparation method of core-shell type multi-effect foliar fertilizer based on collagen polypeptide nanospheres |
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Application publication date: 20170922 |