CN103214317A - Active amino acid water-soluble fertilizer as well as preparation method and application thereof - Google Patents
Active amino acid water-soluble fertilizer as well as preparation method and application thereof Download PDFInfo
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- CN103214317A CN103214317A CN2013101822920A CN201310182292A CN103214317A CN 103214317 A CN103214317 A CN 103214317A CN 2013101822920 A CN2013101822920 A CN 2013101822920A CN 201310182292 A CN201310182292 A CN 201310182292A CN 103214317 A CN103214317 A CN 103214317A
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- 150000001413 amino acids Chemical class 0.000 title claims abstract description 120
- 238000002360 preparation method Methods 0.000 title claims abstract description 26
- -1 compound amino acid Chemical class 0.000 claims abstract description 22
- 235000015097 nutrients Nutrition 0.000 claims abstract description 16
- 230000001737 promoting effect Effects 0.000 claims abstract description 11
- 150000008575 L-amino acids Chemical class 0.000 claims abstract description 6
- 235000001014 amino acid Nutrition 0.000 claims description 128
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 43
- 239000003795 chemical substances by application Substances 0.000 claims description 38
- 238000000034 method Methods 0.000 claims description 32
- 239000002131 composite material Substances 0.000 claims description 27
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Landscapes
- Fertilizers (AREA)
Abstract
The invention provides an active amino acid water-soluble fertilizer. The fertilizer is composed of the following components in parts by weight: 480-836 parts of an active compound amino acid preparation, 210-410 parts of a compound amino acid microelement chelate, 8-16 parts of a plant nutrient element and 14-26 parts of a plant activator, wherein the effective components of the active compound amino acid preparation comprise L-amino acid with biological activity and bioactive peptides, and the compound amino acid microelement chelate is formed by chelating the active compound amino acid with the microelements in proportion. The invention also provides a preparation method and an application of the fertilizer. Agricultural resources are used as the main raw materials to prepare a new generation of the environmentally friendly amino acid fertilizer by biological fermentation technology, and the fertilizer is applied to the plants, with good plant absorption efficiency. The fertilizer takes effect quickly and has obvious effects of initiating roots, promoting seedlings, strengthening rods, increasing the resistance ability, preventing cracking and increasing the yield and the quality; and moreover the fertilizer is safely applied, and widely used in green organic agriculture and agricultural facilities.
Description
Technical field
The invention belongs to technical field of agricultural fertilizers, particularly a kind of active amino acid water-soluble fertilizer and its production and application.
Background technology
Along with improving constantly of human living standard, people are also more and more higher to the specification of quality of farm crop and agricultural byproducts thereof, only the requirement of leaning on traditional planting patterns and long-term application chemical fertilizer can not satisfy green organic food supply and production is so the development and use of vegetable protein resource are the emphasis of producing the development of resources of the water-soluble fertilizer of amino acid.China is the native place of soybean, aboundresources, so the soya products exploitation, the soybean deep processing will be the most important thing of amino acid water-soluble fertilizer systems engineering.
In agriculture production, there is the amino acids material of multiple biocatalysis using at present.Nearest research has proved that also Lalgine, amino acid, silicic acid, Whitfield's ointment, propiconazole, trinexapac-ethyll, Bian Ji purine etc. are very promising.These all materials produce useful effect by hormone effect, raising antioxidant levels and raising resistance.
Amino acid is the basic comprising unit of protein and enzyme.Its synthetic and absorption is the key of cells survival.The amino acid overwhelming majority on the agricultural materials market derives from proteinic degradation production: amino acid and peptide.Proteinic hydrolysis has following approach: chemical process decomposition, enzyme decomposition, acetate anaerobic digestion, microwave decomposition etc.The method of the animal source protein hydrolyzate that obtains by chemical treatment is at first used in agricultural, and its product also is the agricultural amino acid main flow raw material on the current global agricultural materials market.According to the experience of main flow producer (Italian SICIT company), chemical hydrolysis allows the accurate control to proteolysis degree and free aminoacid content.These ideas have been identified and have used as controlling factor in the biostimulation character of protein hydrolyzate.
Enzyme is the biologically active material, can specifically act on the peptide compound, just forms on the chain between proteinic amino acid.Introduce according to obtain amino acid whose manufacturers (Hispanic Bioberica) with the enzymolysis approach, the main difference that obtains a kind of amino acid whose method and acidolysis and alkaline hydrolysis method by enzymolysis protein matter is: (1) does not have amino acid degradation, and some amino acid such as tryptophane and Gelucystine are to degrade under extreme acid-basicity or hot conditions very easily.When enzyme is done the time spent under mild condition, final product comprises all kinds amino acid of different quantities; (2) chemical transformation of L type to the D type can not take place in amino acid; (3) high de-agglomeration rate: any protein raw material all can obtain high total free aminoacids ratio by the enzymolysis mode.
Nineteen sixty-eight, in Italy, amino acid and peptide at first are introduced in agricultural and test.At present, gondola first commercial prod is all being sold in all over the world locality or transnational company.Now, many based on amino acid whose product as a kind of " pure natural organic fertilizer " production and selling, but all be under the name of biocatalysis, to do.Simultaneously in West Europe, the North America, many different researchs are being carried out, but Eastern Europe is more more.The not same-action and the mechanism of action of these amino acid and peptide pointed out in these researchs.Discover that they are playing the part of an important role in the plant physiology activity.Therefore, they stimulate influences mechanism and some is regulated the activity influence that nutrition transforms the enzyme of metabolic process plant-growth.
Scientific research shows that crop is to blade spraying or irritate the amino acid that root uses and very high absorption is arranged, transport and utilize ability.On the agricultural materials market, there are many amino acid products as biological catalyst, appropriate use, some is output, quality and the resistance that can improve agricultural-food, and some is can not.So the present invention with regard to the ratio that is based on amino acid kind, concentration and some specific amino acids and peptide and the mechanism that influences plant-growth study.
Summary of the invention
In order to address the above problem, the object of the present invention is to provide a kind of active amino acid water-soluble fertilizer.Another object of the present invention is to provide the preparation method of above-mentioned active amino acid water-soluble fertilizer.
In order to realize the object of the invention, a kind of active amino acid water-soluble fertilizer provided by the invention, it is liquid or solid shaped dosage forms, and is water-soluble, contains following components in part by weight:
Preferably, fertilizer of the present invention contains following components in part by weight:
More preferably, fertilizer of the present invention contains following components in part by weight:
Wherein, described active mixed amino acid, its effective constituent is the L-amino acid and the bioactive peptide of biologically active, by high-temperature denatured dregs of beans → pulverized 120 mesh sieve samples → by the weight ratio 1:8-9 with water add water adjusting → adjusting ph value 6.5-7.5 → add composite fungus agent → with the NaOH solution adjust pH 6.5-7.5 of 2mol/L be hydrolyzed → enzymolysis solution → supernatant liquor considers by pressure filter and removes residue filtration → active amino acid supplement.
Particularly, active mixed amino acid prepares by the following method:
1) agricultural byproducts are crossed 120 mesh sieves, substrate by weight: water is that the ratio of 1:8-9 adds water, and described agricultural byproducts are high-temperature denatured dregs of beans or any proteinaceous agriculture organism and agricultural byproducts;
2) preparation composite fungus agent: aspergillus oryzae (Aspergillus oryzae), trichoderma harziarum (harzianum), subtilis (Bacillus subtilis) are mixed and made into composite fungus agent by the weight ratio of 1.5-2.5:0.5-1.5:0.5-1.5;
3) composite fungus agent is added in the agricultural byproducts that step 1) obtains, adopt enzyme hydrolysis method that the agricultural byproducts enzymolysis is obtained active mixed amino acid.
Preferably, above-mentioned three kinds of equal weight proportions are 2:1:1.
Step 2) viable count of aspergillus oryzae (Aspergillus oryzae) is 0.5 * 10 in
9-1.5 * 10
9Cfu/g, the viable count of trichoderma harziarum (harzianum) is 0.2 * 10
9-0.8 * 10
9Cfu/g, the viable count of subtilis (Bacillus subtilis) is 0.2 * 10
9-0.8 * 10
9Cfu/g.
Composite fungus agent is as the precursor of compound protease in the described step 3), add in the mixture of entry and agricultural byproducts after, the effect of performance compound protease, so the ratio work that composite fungus agent adds the enzyme of its generation of back should be 16000-20000u/g.The enzyme activity unit definition: under 25 ℃ of conditions, reaction 1min discharges the needed enzyme amount of 1nmol product.U represents the unit of activity of enzyme.U/g is than work, the enzyme activity that every gram zymoprotein is had, and unit is u/g, high more then enzyme is pure more than living.
The hydrolysis temperature of step 3) is 50-60 ℃, and enzymolysis time is 2-4h, and the pH value is 6.5-7.5.
Stop hydrolysis during for 31.8-41.7% when protein degree in the step 3).
Described chelated trace element of compound amino acid is that above-mentioned active mixed amino acid, borax, Zinc Sulphate Heptahydrate form according to the ratio chelating of weight ratio 2-3:0.5-1.5:0.5-1.5;
Preferably, described chelated trace element of compound amino acid is that above-mentioned active mixed amino acid, borax, Zinc Sulphate Heptahydrate form according to the ratio chelating of weight ratio 2.5:1:1.
Described chelated trace element of compound amino acid is to react after measuring active aminoacids complex according to the above ratio respectively and above-mentioned inorganic microelement mixing, and whole anti-temperature is carried out at 55-65 ℃, time 0.5-1.0 hour, and control pH value 6.5-7.5;
Described plant nutrient is a urea;
Described plant promoting agent is sodium nitrophenolate 0.1-0.3g/L, and a-Nafusaku 0.03-0.07g/L is formulated by the two volume ratio 3-5:0.5-1.5.
The preparation method of described active amino acid water-soluble fertilizer stirs abundant mixing by aforementioned ratio with active amino acid supplement, chelated trace element of compound amino acid, plant nutrient and plant promoting agent and is processed into.Be about to active mixed amino acid 480-836 part according to the weight part meter; Chelated trace element of compound amino acid 210-410 part; Plant nutrient 8-16 part; Plant promoting agent 14-26 part mixing and stirring prepares.
Preferably, it is with the active mixed amino acid 510-806 part according to the weight part meter; Chelated trace element of compound amino acid 260-360 part; Plant nutrient 9-15 part; Plant promoting agent 16-24 part mixing and stirring prepares.
More preferably, it is with according to 658 parts of the active mixed amino acids of weight part meter; 310 parts of chelated trace element of compound amino acid; 12 parts of plant nutrients; 20 parts of mixing and stirring of plant promoting agent prepare.
Described active amino acid water-soluble fertilizer is liquid or powdery or particulate state formulation.
Active mixed amino acid is to utilize the agricultural byproducts resource to be main raw material, by high-tech of new generation, multi-functional, the environment-friendly type amino-acid liquid of biofermentation technique preparation; The main raw material of described agricultural byproducts resource is high-temperature denatured dregs of beans or any proteinaceous agriculture organism and agricultural byproducts thereof.
Described biofermentation technique is an enzyme process for the conjugated protein enzyme hydrolysis method.The enzyme process reaction temperature and, few, the production process of facility investment do not have corrosion and pollutes, and do not produce harmful side product, quality product is best, is the most advanced method of present protein hydrolysate.Zymyhydrolyzed protein matter is by taking all factors into consideration degree of hydrolysis and production cost, to reach the requirement of low-cost high-efficiency.
The present invention also provides above-mentioned active amino acid water-soluble fertilizer in application in agriculture.Product of the present invention is according to fertilizer requirement for crop growth rule and leaves and stems nutrient absorbing and conversion capability, carries out the conditioning of nutrient supply and transformation function and a kind of broad spectrum type amino acid water-soluble fertilizer of development.Adopted up-to-date biological enzymolysis technology, tens kinds of nutrition elite that the biopolymerization crop is required, be rich in bioactive peptide, L-amino acid, also include nitre attitude, ammonium attitude, amidonitrogen simultaneously, rapid available phosphorus, potassium and chelating attitude iron, manganese, zinc, copper, boron, xanthohumic acid, probiotics, plant regulation agent etc., compatible property is good between each nutrient, is easily absorbed by crop, have antibacterial disease-resistant, improve the plant materials immunologic function, regulate the effect of plant growth.The raw material that the present invention adopts is easy to get, and preparation and using method are simple, operation is easily gone.
The advantage of product of the present invention is:
1, product utilization agricultural byproducts resource of the present invention is a main raw material, by high-tech of new generation, multi-functional, the environment-friendly type active amino acid liquid fertilizer of biofermentation technique production.It is rich in bioactive peptide, L-amino acid, calcium constituent, spontaneous growth active substance.Have effects such as significant root of hair, short seedling, strong bar, degeneration-resistant, anticracking, volume increase, high-quality, can be widely used in industrialized agriculture and green agriculture.
2, product of the present invention is multiple agricultural-food fermentative degradation material, active amino acid content height, and the crop assimilated efficiency is good, instant effect, and use safety, be a kind of environmental friendly fertilizer.
3, product of the present invention have hestening rooting, bloom, bear fruit, anti-dehiscent fruit, the effect of hyperchromic pool, and good resistance (freeze injury, drought injury, wet injury, disease etc.) is arranged.Especially can make the plant that is injured recover growth rapidly.
4, product of the present invention can promote melon, really, the vegetables maturation, go on the market ahead of time about a week, and can prolong about one month collection period, make crop yield 10%-0%.Simultaneously obviously improve quality of agricultural product, help fresh-keeping, storage.
5, product of the present invention adds in the soil body by adding composite fungus agent, micro-flora in the soil is distributed, the environment of an activation is improved, the generation of increased activity and a large amount of derivatives, chelating or obstruct heavy metal have the function that rehabilitating soil pollutes to the direct harm of crop.
6, product of the present invention combines the organic-inorganic glue complex body of formation by dashing to be exerted in the soil body with mineral colloid, has the soil of improvement reason, voltinism energy, strengthens soil water-retaining, fertilizer-preserving ability.
7, the bioactive peptide in the product of the present invention, L-amino acid etc. have stable crop cell potential of hydrogen and improve ability resistant to lodging.
8, active amino acid water-soluble fertilizer of the present invention is liquid or solid shaped dosage forms, and application process is simple and practical, can spray on crop field top, can be used as towards spraying fertilizer again.
9, the field effect of active amino acid water-soluble fertilizer of the present invention is good.
Three bacterial classification sources that the present invention relates to are as follows respectively:
1, aspergillus oryzae (Aspergillus oryzae) China Committee for Culture Collection of Microorganisms agricultural microorganism center ACCC30155;
2, trichoderma harziarum (harzianum) China Committee for Culture Collection of Microorganisms agricultural microorganism center ACCC30371;
3, subtilis (Bacillus subtilis) China Committee for Culture Collection of Microorganisms agricultural microorganism center SCTCC1132.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.Without departing from the spirit and substance of the case in the present invention, modification or replacement to the inventive method, step or condition are done all belong to protection scope of the present invention.
If do not specialize the conventional means that used technique means is well known to those skilled in the art among the embodiment.If do not specialize, used chemical reagent is commercially available among the embodiment.
The preparation (1) of embodiment 1 active amino acid water-soluble fertilizer
1, composite fungus agent prescription
The effective constituent of its precursor of the compound protease composite fungus agent that to be aspergillus oryzae, trichoderma harziarum, subtilis be mixed and made into by the part by weight of 2:1:1; The viable count of aspergillus oryzae is 1.0 * 10 in the microbial inoculum
9Cfu/g, the viable count of trichoderma harziarum are 0.5 * 10
9Cfu/g, the viable count of subtilis are 0.5 * 10
9Cfu/g.
2, the preparation of active mixed amino acid
Utilizing the high-temperature denatured dregs of beans of agricultural byproducts resource (buy from China Oil and Food Import and Export Corporation's Huanghai Sea grain and oil industry (Shandong) company limited, the said firm is positioned at north, Rizhao City PORT OF LANSHAN, Shandong) for main raw material, is that the conjugated protein enzyme hydrolysis method carries out enzymatic hydrolysis by biofermentation technique.High-temperature denatured dregs of beans is mixed by weight 1:9 with water, add composite fungus agent, requiring the addition of control enzyme (being the composite fungus agent of present embodiment) is 18000u/g; Hydrolysis temperature is controlled at 55 ℃; Enzymolysis time is decided to be 4h; Regulation and control ph value is 7.0; When protein degree reaches 36.8% in the high-temperature denatured dregs of beans, stop hydrolysis, clarification, supernatant liquor is considered machine filter except that the residue clarification by pressing, and supernatant liquor is stand-by.
3, the preparation of chelated trace element of compound amino acid
The active mixed amino acid, borax, the Zinc Sulphate Heptahydrate that make in the step 2 are formed according to the part by weight chelating of 2.5:1:1; Its inner complex is to measure active aminoacids complex respectively by weight proportion and above-mentioned inorganic microelement reacts, 60 ℃ of entire reaction constant temperature, time 0.5h, control pH value 7.0.
4, the preparation of active amino acid water-soluble fertilizer
Will be according to 658 parts of the active mixed amino acids of weight part meter; 310 parts of chelated trace element of compound amino acid; 12 parts in plant nutrient urea; 20 parts of (the two volume ratio is 3:0.5 for sodium nitrophenolate 0.2g/L, a-Nafusaku 0.04g/L) mixing and stirring of plant promoting agent prepare.
The preparation (2) of embodiment 2 active amino acid water-soluble fertilizers
1, composite fungus agent prescription
The effective constituent of its precursor of the compound protease composite fungus agent that to be aspergillus oryzae, trichoderma harziarum, subtilis be mixed and made into by the part by weight of 2:1.5:1.5; The viable count of aspergillus oryzae is 0.8 * 10 in the microbial inoculum
9Cfu/g, the viable count of trichoderma harziarum are 0.6 * 10
9Cfu/g, the viable count of subtilis are 0.6 * 10
9Cfu/g.
2, the preparation of active mixed amino acid
Utilizing the high-temperature denatured dregs of beans of agricultural byproducts resource (buy from China Oil and Food Import and Export Corporation's Huanghai Sea grain and oil industry (Shandong) company limited, the said firm is positioned at north, Rizhao City PORT OF LANSHAN, Shandong) for main raw material, is that the conjugated protein enzyme hydrolysis method carries out enzymatic hydrolysis by biofermentation technique.High-temperature denatured dregs of beans is mixed by weight 1:8 with water, add composite fungus agent, requiring the addition of control enzyme (being the composite fungus agent of present embodiment) is 20000u/g; Hydrolysis temperature is controlled at 50 ℃; Enzymolysis time is decided to be 3h; Regulation and control ph value is 7.5; When protein degree reaches 41.7% in the high-temperature denatured dregs of beans, stop hydrolysis, clarification, supernatant liquor is considered machine filter except that the residue clarification by pressing, and supernatant liquor is stand-by.
3, the preparation of chelated trace element of compound amino acid
Active mixed amino acid, borax, Zinc Sulphate Heptahydrate form according to the part by weight chelating of 3:1.5:1.5; Its inner complex is to measure active aminoacids complex respectively by weight proportion and above-mentioned inorganic microelement reacts, and the entire reaction temperature adjusting is at 55 ℃, time 1.0h, control ph value 7.5.
4, the preparation of active amino acid water-soluble fertilizer
Will be according to 580 parts of the active mixed amino acids of weight part meter; 388 parts of chelated trace element of compound amino acid; 9 parts in plant nutrient urea; 23 parts of (the two volume ratio is 5:0.5 for sodium nitrophenolate 0.1g/L, a-Nafusaku 0.03g/L) mixing and stirring of plant promoting agent prepare.
The preparation (3) of embodiment 3 active amino acid water-soluble fertilizers
1, composite fungus agent prescription
The effective constituent of its precursor of the compound protease composite fungus agent that to be aspergillus oryzae, trichoderma harziarum, subtilis be mixed and made into by the part by weight of 1.5:1.5:1.4; The viable count of aspergillus oryzae is 0.5 * 10 in the microbial inoculum
9Cfu/g, the viable count of trichoderma harziarum are 0.8 * 10
9Cfu/g, the viable count of subtilis are 0.7 * 10
9Cfu/g.
2, the preparation of active mixed amino acid
Utilizing the high-temperature denatured dregs of beans of agricultural byproducts resource (buy from China Oil and Food Import and Export Corporation's Huanghai Sea grain and oil industry (Shandong) company limited, the said firm is positioned at north, Rizhao City PORT OF LANSHAN, Shandong) for main raw material, is that the conjugated protein enzyme hydrolysis method carries out enzymatic hydrolysis by biofermentation technique.High-temperature denatured dregs of beans is mixed by weight 1:8.6 with water, add composite fungus agent, require the addition 19000u/g of control enzyme (being the composite fungus agent of present embodiment); Hydrolysis temperature is controlled at 60 ℃; Enzymolysis time is decided to be 2h; Regulation and control ph value is 6.5; When protein degree reaches 39.3% in the high-temperature denatured dregs of beans, stop hydrolysis, clarification, supernatant liquor is considered machine filter except that the residue clarification by pressing, and supernatant liquor is stand-by.
3, the preparation of chelated trace element of compound amino acid
Active mixed amino acid, borax, Zinc Sulphate Heptahydrate form according to the part by weight chelating of 3:1:1; Its inner complex is to measure active aminoacids complex respectively by weight proportion and above-mentioned inorganic microelement reacts, and the entire reaction temperature adjusting is at 60 ℃, time 1.0h, control pH value 7.5.
4, the preparation of active amino acid water-soluble fertilizer
Will be according to 680 parts of the active mixed amino acids of weight part meter; 288 parts of chelated trace element of compound amino acid; 11 parts in plant nutrient urea; 21 parts of (sodium nitrophenolate 0.3g/L, a-Nafusaku 0.07g/L, the two volume ratio 4:1.5) mixing and stirring of plant promoting agent prepare.
The preparation (4) of embodiment 4 active amino acid water-soluble fertilizers
1, composite fungus agent prescription
The effective constituent of its precursor of the compound protease composite fungus agent that to be aspergillus oryzae, trichoderma harziarum, subtilis be mixed and made into by the part by weight of 2.5:1:1; The viable count of aspergillus oryzae is 1.5 * 10 in the microbial inoculum
9Cfu/g, the viable count of trichoderma harziarum are 0.25 * 10
9Cfu/g, the viable count of subtilis are 0.25 * 10
9Cfu/g.
2, the preparation of active amino acid supplement
Utilizing the high-temperature denatured dregs of beans of agricultural byproducts resource (buy from China Oil and Food Import and Export Corporation's Huanghai Sea grain and oil industry (Shandong) company limited, the said firm is positioned at north, Rizhao City PORT OF LANSHAN, Shandong) for main raw material, is that the conjugated protein enzyme hydrolysis method carries out enzymatic hydrolysis by biofermentation technique.High-temperature denatured dregs of beans is mixed by weight 1:8.9 with water, add composite fungus agent, require the addition 17000u/g of control enzyme (being the composite fungus agent of present embodiment); Hydrolysis temperature is controlled at 55 ℃; Enzymolysis time is decided to be 2.5h; Regulation and control ph value is 7.0; When protein degree reaches 38.6% in the high-temperature denatured dregs of beans, stop hydrolysis, clarification, supernatant liquor is considered machine filter except that the residue clarification by pressing, and supernatant liquor is stand-by.
3, the preparation of chelated trace element of compound amino acid
Active mixed amino acid, borax, Zinc Sulphate Heptahydrate form according to the part by weight chelating of 2:1.5:1.5; Its inner complex is to measure active aminoacids complex respectively by weight proportion and above-mentioned inorganic microelement reacts, and the entire reaction temperature adjusting is at 65 ℃, time 0.5h, control ph value 7.5.
4, the preparation of active amino acid water-soluble fertilizer
Will be according to 758 parts of the active mixed amino acids of weight part meter; 210 parts of chelated trace element of compound amino acid; 14 parts of plant nutrients; 18 parts of (sodium nitrophenolate 0.25g/L, a-Nafusaku 0.05g/L, the two volume ratio 4:1) mixing and stirring of plant promoting agent prepare.
The manure trial of embodiment 5 in the romaine lettuce plantation
By regular field test, the active amino acid water soluble fertilizer that uses embodiment 1 to make is observed its fertilizer efficiency on romaine lettuce, evaluating this fertile effect of increasing production and economic benefit, for the demonstration of this fertilizer, promote scientific basis is provided.
1 materials and methods
1.1 test site
Test (in booth) in the vegetables field of Agro-ecology Technology Park, the Gu Gang of Liuyang City town is carried out, and soil fertility is better-than-average, and irrigation and drainage are convenient.
1.2 test materials
For studying thing: the balling romaine lettuce, available from academy of agricultural sciences, Hunan Province means of agricultural production service department.
For trying fertilizer: the amino acid water soluble fertilizer that the embodiment of the invention 1 makes, this fertilizer are liquid, free aminoacid content 〉=100g/L, micronutrient levels 〉=20g/L, the non-water solubles≤50g/L, pH value 3.0-7.0,6.5 yuan/kg of market price; Norway blue compound fertilizer (N: P: K
2O=16: 0: 14), 3500 yuan/t of market price; Skies production urea (N 〉=46%), 2500 yuan/t of market price.
1.3 test is handled
Handle 1: each per hectare is watered and is executed urea decrement 20% in the fertilizer 150kg+ conventional fertilizer application that embodiment 1 makes; Handle 2: each per hectare is watered and is executed urea decrement 20% in the embodiment 1 fertilizer 225kg+ conventional fertilizer application; Handle 3: conventional fertilizer application; Handle 4: water equivalent clear water (CK)+conventional fertilizer application decrement 20%.
1.4 test design
District's group arrangement is at random adopted in test, handles 3 times for 4 and repeats.Zone leader 8m, wide 1.5m, the minizone is every 0.5m, sub-district area 12m
2, every sub-district plants 20 row, and every row is planted 4 strains, totally 80 strains, the plantation specification is line-spacing 37.5cm * spacing in the rows 40cm, per hectare 66705 strains.Transplanted on October 4th, 2010, transplanted seedling is that the dish that floating seedlings is bred is grown seedlings, and adopts row bar such as individual plant to plant during seedling 2 leaves 1 heart.Fertilization time and quantity see table 1 for details.October 14, each was handled with the 000 times of liquid spraying in Pentium 4, good field, October 29, each was handled with 500 times of liquid of phytokinin+must 500 times of liquid of good fortune+1500 times of liquid spraying insects of Qingling go out, spray enemy's power October 24 and take off 3000 times of liquid 1 time, sprayed enemy's power on October 26 and take off 1500 times of liquid of 1500 times of liquid+agricultural streptomycins of 3000 times of liquid+triazolones 1 time, November 14 gathered in the crops.Fertilising quantity and time see Table 1-1.
2 results and analysis
2.1 the different treatment economical character is analyzed
The amino acid water soluble fertilizer different treatment that embodiment 1 makes sees Table 1-2 to the influence of romaine lettuce Other Main Agronomic Characters.From table 1-2 as seen, processing 1,2 is compared with processing 3,4, and it is strong that growing period shows as plant, and growing way is strong, and color is dark green, and color and luster is good, and commodity property is good.Through investigation, handle 1,2 leaf-head during results than handling 3,4 difference high 0.6cm, 0.8cm.The horizontal stem processing 2 of leaf-head is the wideest, respectively than handling 1, handle 3, handling 4 wide 0.5cm, 0.3cm, 0.7cm.The single leaf-head net weight processing 2 of individual plant is the highest, secondly is to handle 1; Handle 2 ratio processing 1, processing 3, processing 4 high 0.01kg, 0.05kg, 0.07kg respectively; The clean vegetables rate also is that processing 2 is the highest.
2.2 different treatment volume analysis
The different treatment yield result sees Table 1-3.From table 1-3 as seen, it is the highest to handle 2 output, output per hectare 56667.0kg, and than handling 1, handle 3, handling 4 difference high 833.4kg, 2750.1kg, 4750.1kg, output occupies the 1st; Handle 1 output and take second place, per hectare is produced 55833.6kg, and than handling 3, handling 4 difference high 1916.7kg, 3916.7kg, output occupies the 2nd.
2.3 variance analysis
The results of analysis of variance (table 1-4) shows that F=13.057＞F0.01=9.73 between processing shows to have utmost point significant difference between processing.Carry out multiple comparisons (showing 1-5) with new multipole difference SSR, the result shows that processing 2 has utmost point significant difference with processing 3, processing 4, with processing 1 indifference; Processing 1 has utmost point significant difference with processing 4, does not have significant difference with processing 3; Indifference between processing 3 and the processing 4.
2.4 different treatment Economic and Efficiency Analysis
The amino acid water soluble fertilizer of using embodiment 1 on romaine lettuce has good economic benefit, the highest to handle 2 economic benefits, per hectare is than handling 4(CK) net output is high 10420.05 yuan, than handling the 3(routine) net output is high 5600.25 yuan, and is higher 1596.0 yuan than handling 1;
Table 1-1 amino acid water-soluble fertilizer fertilization time, quantity
The Other Main Agronomic Characters of table 1-2 different treatment
The volume analysis of table 1-3 different treatment
Table 1-4 variance analysis
| Source of variation | DF | SS | MS | F | F0.05 | F0.01 |
| Handle | 3 | 57.667 | 19.222 | 13057 | 4.757 | 9.78 |
| District's group | 2 | 18.500 | 19.22 | 6.283 | 5.143 | 10.925 |
| Error | 6 | 8.833 | 1.472 | ? | ? | ? |
| Total variation | 11 | 85.000 | ? | ? | ? | ? |
The new multipole difference of table 1-5 relatively
The Economic and Efficiency Analysis of table 1-6 different treatment
Annotate: the romaine lettuce price is calculated by 2.5 yuan/kg of market price.
Next is to handle 1, and the per hectare net output is than handling 4(CK) high 8824.05 yuan, than handling the 3(routine) high 4004.25 yuan.
3 discuss
The amino acid water soluble fertilizer that the embodiment of the invention 1 makes is used effect of increasing production on Liuyang City romaine lettuce crop extremely remarkable, and have advantages such as easy to use, safe, environmental protection, long-term application should can improve soil organic matter content by fertilizer, improve soil physical and chemical property, can in Liuyang City's romaine lettuce production, promote the use of.Application process: per hectare is watered at every turn and is executed urea decrement 20% in the amino acid water soluble fertilizer 225kg+ conventional fertilizer application of the present invention.
The manure trial of embodiment 6 on tomato planting
Foliage fertilizer can replenish crop alimentary, regulates plant growth, for the practical application effect of checking amino acid water-soluble fertilizer of the present invention on tomato, is subjected to the trust at clay fertilizer station, Hubei Province, has carried out application test on the tomato of Shashi.
1 materials and methods
1.1 test materials
For the active amino acid water-soluble fertilizer that examination fertilizer makes for embodiment 2, the safe paddy biotech inc in Hunan is produced, amino acid 〉=100g/L, copper+zinc+manganese+iron 〉=20g/L, and product forms is an aqua; Educate No. 3 tomatoes of space for studying thing for boat, provide by agricultural science popularization demonstration garden, Anhui Lu'an City gold soil.This kind plant height 80-100cm, 6-7 sheet leaf and give birth to 1 inflorescence, are medium variety morning, strong stress resistance, growth potential is prosperous, fruit is high circular, pink, distinguish the flavor of sweet not sour, quality is good, pulp is thick, storage tolerance and transportation; About general single fruit weight 200g, maximum reached at 500g, even, the neat appearance of fruit size, 667m
2Output can reach 5000-7500kg.
1.2 test method
Test is located at Shashi agricultural experiment station, is silty loam for examination soil, and soil fertility is medium.Soil sampling is measured before the test, organic content 24.58g/kg, full nitrogen 1.35g/kg, available phosphorus 25.9g/kg, available potassium 96.5g/kg, pH value 7.7.
1.3 test design
3 processing are established in test, 1. handle 1: base manure, topdress and use by local conventional, do not use foliage fertilizer; 2. handle 2: each all with the equivalent clear water of handling 3, at the same time between foliage-spray, other measures are with processing 1; 3. handle 3: every 667m
2Convert water 80kg foliage-spray with containing amino acid water-soluble fertilizer 150mL, respectively at spray in seedling stage (before the bud differentiation) 1 time, fruit expanding period spray 2 times (8d at interval), other measures are with handling 1 at every turn.Randomized block design is adopted in test, repeats totally 9 sub-districts, sub-district area 20m 3 times
2Tomato was sowed on November 21st, 2009, field planting on February 2 in 2010, every 667m
2Plant 2600 strains, be 14 days-July 20 May in 2010 harvesting time.Every 667m
2Execute well-rotted farmyard manure 3500kg, efficient compound fertilizer 50kg makes base manure; In the 1st fringe fruit stone peach when size, is in conjunction with watering every 667m
2Impose urea 6.5kg, the 2nd, 3 fringes fruit is expanding stage rapidly, each every 667m
2Impose urea 7.5kg, topdress altogether 3 times.Adopt single stalk training, every fringe stays 3-4 of fruit.Except that spraying foliage fertilizer, each rich water of handling is planned strategies for, the prevention and control of plant diseases, pest control, agronomic measures are all consistent.The foliage fertilizer time of spraying is January 27, March 15,23 days.
2 results and analysis
2.1 influence to output
By table 2-1 as can be known, the amino acid water-soluble fertilizer that embodiment 2 makes (handling 3) mean yield is 134.28kg, compare with the check plot of conventional fertilizer application (handling 1) and the check plot (handling 2) that sprays the equivalent clear water, tomato yield increases 9.62kg and 9.13kg respectively, amounts to 667m
2Output increases 320.83kg and 304.49kg respectively, and stimulation ratio is respectively 7.717% and 7.295%.For check test result's accuracy, data have been carried out variance analysis, volume variance reaches utmost point conspicuous level between processing, and does not have significant difference (seeing Table 2-2) between repeating.
The LSD test result shows, under the situation of Routine Management unanimity, the spray clear water does not have significant difference with not spraying on tomato, yet contains amino acid water-soluble fertilizer and spray, the contrast of not spraying clear water all reached effect of increasing production (seeing Table 2-3) extremely significantly.
Table 2-1 tomato sprays the influence of foliage fertilizer to output
Table 2-2 tomato sprays the yield result analysis of variance table of foliage fertilizer test
| Source of variation | Degree of freedom | Sum of squares | Root mean square value | The F value | Significant difference |
| Between repetition | 2 | 0.285 | 0.14 | 0.11 | ? |
| Between processing | 2 | 176.14 | 88.07 | 65.85 | ** |
| Testing error | 4 | 5.35 | 1.34 | ? | ? |
| Total variation | 8 | 181.78 | ? | ? | ? |
Table 2-3 tomato sprays the multiple comparisons of the yield result of foliage fertilizer test
2.2 influence to the tomato single fruit weight
By table 2-4 as can be known, the amino acid water-soluble fertilizer of embodiment 2 (handling 3) tomato mean fruit weight is 235.80g, with conventional fertilizer application (handle 1) and spray equivalent clear water (handling 2) and compare, the tomato single fruit weight has increased 15.52g and 15.29g respectively, and rate of body weight gain is respectively 7.05% and 6.93%.
The mean fruit weight that table 2-4 tomato sprays the foliage fertilizer test is table as a result
For check test result's accuracy, data have been carried out variance analysis, volume variance reaches utmost point conspicuous level (F=57.04** between processing) between processing, and does not have significant difference (seeing Table 2-5) between repeating.
Table 2-5 tomato sprays the yield result analysis of variance table of foliage fertilizer test
| Source of variation | Degree of freedom | The sum of squares root mean square value | The F value | Significant difference |
| Between repetition | 2 | 0.880.44 | 0.11 | ? |
| Between processing | 2 | 474.91237.46 | 57.04 | ** |
| Testing error | 4 | 16.654.16 | ? | ? |
| Total variation | 8 | 492.45 | ? | ? |
The LSD test result shows, under the situation of Routine Management unanimity, the tomato single fruit weight is with the spray clear water and do not spray clear water and do not have significant difference, yet tomato spray contain the amino acid water-soluble fertilizer after spray and the contrast ratio that does not spray clear water, its single fruit gaining effect has all reached extremely significant level (seeing Table 2-6).
Table 2-6 tomato sprays the multiple comparisons of the yield result of foliage fertilizer test
2.3 influence to economic benefit
By 2.5 yuan of calculating of the average every kg price of tomato, the producible economic benefit of different treatment sees Table 2-7.
Table 2-7 Economic and Efficiency Analysis table
By table 2-7 as can be known, on the basis of conventional fertilizer application, tomato sprays and contains the amino acid water-soluble fertilizer tangible the effect of increasing income is arranged, and handles 3 and compares every 667m with contrast 1,2
2New output value is respectively 802.08 yuan, 761.23 yuan, 6 yuan of deduction foliage fertilizer costs, every 667m
2Have a net increase of 796.08 yuan of benefits, 755.23 yuan, input-output ratio reaches 1: 133 and 1: 126, and economic benefit is considerable.
3 conclusions
Under this test conditions, at spray in the seedling stage of tomato (before the bud differentiation) 1 time, fruit expanding period spray 2 times (8d at interval), the amino acid water-soluble fertilizer that embodiment 2 makes, each every 667m
2Consumption 150mL converts water 80kg, can significantly increase single fruit weight and the output of tomato, and difference reaches utmost point conspicuous level.With routine contrast and only spray the equivalent clear water and compare, amount to 667m
2Output has increased 320.83kg and 304.49kg respectively, and stimulation ratio is respectively 7.717% and 7.295%, every 667m
2New output value is respectively 802.08 yuan, 761.23 yuan, 6 yuan of deduction foliage fertilizer costs, every 667m
2Have a net increase of 796.08 yuan of benefits, 755.23 yuan, input-output ratio reaches 1: 133 and 1: 126.As seen, the amino acid water-soluble fertilizer of using embodiment 2 on the tomato has good increase output and increases the effect of benefit.
The manure trial of embodiment 7 on green vegetables
Foliage application is one of important means of regulating plant growth.Foliage application can replenish the required nutrient of leafy vegetable growth fast, promotes the leaf vegetables growth.For verifying the amino acid water-soluble fertilizer of the present invention actual effect that large-scale demonstration is used on green vegetables, be chosen in July, 2009 and carried out experiment and demonstration on the green vegetables, it is as follows now will to test the retribution road.
1 materials and methods
1.1 be located in Changsha Kaifu District Katyuan community leaf vegetables base 1.85 mu of testing ground areas for examination ground experimental tests.
1.2 for examination soil testing field soil is silty loam, soil organic matter content 26.6g/kg, alkali-hydrolyzable nitrogen 101mg/kg, rapid available phosphorus 19.5mg/kg, available potassium 99mg/kg, and soil fertility level is more even.
1.3 for the amino acid water-soluble fertilizer that examination fertilizer embodiment 3 makes, amino acid 〉=100g/L, trace element 〉=20g/L, product forms is a pulvis.
1.4 for studying the little green vegetables of thing, kind is " green excellent No. 1 ", belongs to heat resistant variety, first-filial generation, and plant type is upright, and petiole is wide flat, and leaf is thick, and light green white is fit to sowing in 5～September.
1.5 test is handled with design experiment and is established 3 processing: 1. conventional contrast (CK
1), base topdresses and is undertaken by the conventional service condition in locality, does not use foliage fertilizer and conditioning agent.2. clear water contrasts (CK
2), every mu at every turn to carry out foliage-spray with handling 3. the equivalent clear water contemporaneously, and other measures of fertilizer are with handling 1..3. contain the amino acid water-soluble fertilizer.Convert 800 times of liquid of water 40kg(dilution with containing amino acid water-soluble fertilizer 50g for every mu at every turn) carry out foliage-spray, the back 15d of emerging sprays 1 time, sprays 1 time every 7d again, sprays altogether the time of infertility 2 times, and other measures of fertilizer is with handling 1..Every processing repeats 3 times, and district's group is arranged at random, and same district group is arranged in the same furrow, sub-district area 36m
2, long-width ratio is 2.5: 1, each minizone isolates with ditch.
1.6 the fertilizer 150kg that becomes thoroughly decomposed is executed in experimental plot cultivation management test field, spread fertilizer over the fields 45% compound fertilizer of high concentration (15: 15: 15) 20kg, be July 8 sowing time, handle 3. to spray for the 1st time and contain the amino acid water-soluble fertilizer July 26, sprayed for the 2nd time August 2 and contain the amino acid water-soluble fertilizer, handle 2. spraying the equivalent clear water simultaneously.
2 results and analysis
2.1 the influence to plant trait can be found out by table 3-1, after 3. processing sprays and contain the amino acid water-soluble fertilizer, can promote little green vegetables growth preferably, plant height is than handling 2. (clear water contrast), handling 1. (routine contrast) high by 2.6,3.2cm respectively; Hundred strain dry weights are 9.5g, 2., 1. weigh 2.2 respectively than handling, 2.6g, illustrate spray contain the amino acid water-soluble fertilizer after little green vegetables endotrophic material many, strengthened the ability that high temperature resistance, heat resistanceheat resistant are shone, the resistance enhancing is so growing way is very prosperous.
Table 3-1 plant trait is investigated the result
2.2 singly pull out single receipts to the little green vegetables of every sub-district the August 11 that influences to yields of vegetables, each cell production (seeing Table 3-2), and from final real producing, 3. output is the highest to spray the processing that contains the amino acid water-soluble fertilizer, average 667m
2Output is 1216kg, compares CK
2(clear water), CK
1(routine) average 667m
2Output increases by 130 respectively, 136kg, analyzes by statistics, sprays the output that contains the amino acid water-soluble fertilizer and reaches utmost point significant difference compared with the control.
Table 3-2 respectively handles yield result
2.3 Economic and Efficiency Analysis contains amino acid water-soluble fertilizer cost and is higher than contrast (CK though spray by table 3-3 as can be known
1) (spraying 50 yuan of recruitment meters, 5 yuan of fertilizer meters), but every 667m at every turn
2Output has increased 136kg, every 667m
2All net return has increased by 406.8 yuan, and economic benefit is very obvious.
Table 3-3 Economic and Efficiency Analysis
3 brief summaries
After 3.1 the promotion growth sprays and contains the amino acid water-soluble fertilizer, can promote little green vegetables growth, chlorophyll is synthetic more, blade fertilizer, big, and stem is thick, and nutritious substances accumulation is many, the enhancing of abilities such as high temperature resistance, disease-resistant worm, growing way is vigorous.
To spray the amino acid water-soluble fertilizer effect of increasing production of embodiment 3 on little green vegetables obvious 3.2 increase output, than clear water contrast, conventional contrast every 667m respectively
2Volume increase 130,136kg analyze by statistics, and effect of increasing production reaches utmost point conspicuous level.
After 3.3 increasing benefit sprays the amino acid water-soluble fertilizer of embodiment 3, every 667m
2Though cost has increased by 110 yuan, contrast (routine) 667m
2Output increases 136kg, every 667m
2Income increases 406.8 yuan, produces and throws than reaching 4.1: 1.
The manure trial of embodiment 8 on green cucumber
1, materials and methods
1,1 material
Tested in July, 2010 and be arranged in the old virtuous contracted field in ecological agriculture garden, Liuyang City.Great soil group is a cinnamon soil, and quality is middle earth, and fertility is better-than-average, and soil fertility is even.The soil plough horizon nutrient is: organic 13.8g/kg, alkali-hydrolyzable nitrogen 101.5mg/kg, rapid available phosphorus (P
2O
5) 14.2mg/kg, available potassium (K
2O) 103.8mg/kg.
For studying the thing cucumber, kind is " excellent No. 1 of Tianjin ".
The water-soluble fertilizer of the amino acid that embodiment 4 makes, amino acid 〉=100g/L, Mn+Zn+B 〉=20g/L.
1,2 methods
3 processing are established in this test, and district's group is arranged at random, repeats sub-district area 30m 3 times
2Handle 1: the fertilizer 30mL that conventional fertilizer application+embodiment 4 makes is to water 30kg, respectively sprays 1 time in cucumber seedling stage, florescence, sprays altogether 2 times;
Handle 2: conventional fertilizer application+spray the equivalent clear water 1 same period with processing;
Handle 3: conventional fertilizer application.Test is carried out on the basis of local conventional fertilizer application.Conventional fertilizer application is: every 667m
2Bottom application high-quality fertilizer 4000kg, 14-15-16 compound potassium sulfate fertilizer 80kg, growing period topdresses 4 times, each every 667m
2Impose urea 5kg+ composite fertilizer 8kg.
Experimental field cucumber is live April 20, and distance between rows and hills is 60cm * 35cm, every 667m
2Keep plant 3170 strains.Respectively at spraying solution or clear water on May 10, May 26, began results in strict accordance with the requirement of testing program on June 2, July 28 harvest home.Receive single product respectively that claims in the sub-district for the unit list during results, output is the cumulative production of repeatedly gathering to July 28 June 2, carries out field investigation and species test during results simultaneously.Test is except that by the spraying solution or the clear water of scheme requirement, and other control measures are with general land for growing field crops.
2, result and analysis
2,1 amino acid fertilizer of the present invention becomes the influence of product factor to cucumber
Spray amino acid fertilizer of the present invention and improved the one-tenth product factor of cucumber.
Table 4-1 field investigation and species test cartogram
| Handle density (strain/667m 2） | Per plant number (individual) | Single fruit weight (g) |
| 13170 | 10.9 | 186.8 |
| 23170 | 10.5 | 181.2 |
| 33170 | 10.4 | 181.0 |
Annotate: data are 3 multiple mean numbers of 3 processing in the table.
4-1 can find out from table: processing 1 is compared with processing 2, processing 3, and the per plant number increases by 0.4,0.5 respectively, and single fruit weight increases 5.6g, 5.8g respectively.Explanation sprays per plant number and single fruit weight that amino acid fertilizer that the embodiment of the invention 4 makes can increase cucumber on the basis of conventional fertilizer application.
2,2 spray the influence of amino acid fertilizer of the present invention to cucumber yield
By table 4-2 as can be seen: handle 1 and handle 2 average every 667m
2Volume increase 300kg, stimulation ratio is 5.9%; Processing 2 is compared every 667m with processing 3
2Volume increase 57.8kg, stimulation ratio is 1.1%.Each is handled yield result carry out variance analysis (seeing Table 4-3), volume variance reaches conspicuous level between processing.
Table 4-2 yield result cartogram
Table 4-3 analysis of variance table
| Source of variation | Sum of squares | Degree of freedom | Variance | F | F0.05 | F0.01 |
| Between processing | 448.22 | 2 | 224.11 | 11.54* | 6.94 | 18 |
| Between district's group | 7.05 | 2 | 3.52 | 0.18 | 6.94 | 18 |
| Chance error | 77.69 | 4 | 19.42 | ? | ? | ? |
| Total variation | 532.96 | 8 | ? | ? | ? | ? |
Table 4-4 multiple comparisons table
Employing PLSD method is carried out multiple comparisons (seeing Table 4-4), and volume variance all reaches conspicuous level between processing 1 and processing 2, the processing 3, and volume variance is not remarkable between processing 2 and the processing 3.
3, brief summary
Test-results shows: on the basis of local conventional fertilizer application, the amino acid fertilizer 30mL that makes with embodiment 4 converts water 30kg, respectively sprays 1 time in cucumber seedling stage, florescence, spray altogether 2 times, compare with spraying the equivalent clear water same period, increased per plant number and the single fruit weight of cucumber, average every 667m
2Volume increase 404.4kg, stimulation ratio is 6.7%, volume variance reaches utmost point conspicuous level.
Though above the present invention is described in detail with a general description of the specific embodiments, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, these modifications or improvements all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (11)
1. active amino acid water-soluble fertilizer, contain following components in part by weight:
Described its effective constituent of active mixed amino acid is the L-amino acid of biologically active.
2. fertilizer as claimed in claim 1 is characterized in that, contains following components in part by weight:
3. fertilizer as claimed in claim 1 is characterized in that, contains following components in part by weight:
4. fertilizer according to claim 1 is characterized in that described active mixed amino acid makes by following steps,
1) agricultural byproducts are crossed 120 mesh sieves, are substrate with agricultural byproducts, and by substrate: water is that the weight ratio of 1:8-9 is mixed;
2) preparation composite fungus agent: aspergillus oryzae (Aspergillus oryzae), trichoderma harziarum (harzianum), subtilis (Bacillus subtilis) are pressed 1.5-2.5:0.5-1.5:0.5-1.5, weight ratio be mixed and made into composite fungus agent;
3) composite fungus agent is added in the agricultural byproducts that step 1) obtains, adopt enzyme hydrolysis method that the agricultural byproducts enzymolysis is obtained active mixed amino acid.
5. fertilizer as claimed in claim 4 is characterized in that step 2) in the viable count of aspergillus oryzae (Aspergillus oryzae) be 0.5 * 10
9-1.5 * 10
9Cfu/g, the viable count of trichoderma harziarum (harzianum) is 0.2 * 10
9-0.8 * 10
9Cfu/g, the viable count of subtilis (Bacillus subtilis) is 0.2 * 10
9-0.8 * 10
9Cfu/g.
6. fertilizer as claimed in claim 4 is characterized in that, the hydrolysis temperature of step 3) is 50-60 ℃, and enzymolysis time is 2-4h, and the pH value is 6.5-7.5.
7. fertilizer according to claim 1 is characterized in that, described chelated trace element of compound amino acid is that active mixed amino acid, borax, Zinc Sulphate Heptahydrate form by the weight ratio chelating of 2-3:0.5-1.5:0.5-1.5.
8. as fertilizer as described in the claim 7, it is characterized in that the chelatropic reaction temperature is 55-65 ℃, 0.5-1.0h, the pH value is 6.5-7.5.
As claim 1-8 arbitrary as described in fertilizer, it is characterized in that described plant nutrient is a urea.
As claim 1-8 arbitrary as described in fertilizer, it is characterized in that described plant promoting agent is sodium nitrophenolate 0.1-0.3g/L, a-Nafusaku 0.03-0.07g/L, by the two volume ratio 3-5:0.5-1.5 formulated.
11. each described fertilizer of claim 1-8 is characterized in that, described fertilizer is liquid or powdery or particulate state formulation.
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Denomination of invention: Active amino acid water-soluble fertilizer as well as preparation method and application thereof Effective date of registration: 20160902 Granted publication date: 20140709 Pledgee: Hunan Wangcheng Rural Commercial Bank branch Limited by Share Ltd. Lugu Pledgor: HUNAN TAIGU BIOTECHNOLOGY Co.,Ltd. Registration number: 2016430000031 |
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Change date: 20170731 Registration number: 2016430000031 Pledgee after: Changsha rural commercial bank Limited by Share Ltd. Lugu branch Pledgee before: Hunan Wangcheng Rural Commercial Bank branch Limited by Share Ltd. Lugu |
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Date of cancellation: 20170809 Granted publication date: 20140709 Pledgee: Changsha rural commercial bank Limited by Share Ltd. Lugu branch Pledgor: HUNAN TAIGU BIOTECHNOLOGY Co.,Ltd. Registration number: 2016430000031 |
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Denomination of invention: Active amino acid water-soluble fertilizer as well as preparation method and application thereof Effective date of registration: 20170816 Granted publication date: 20140709 Pledgee: Changsha rural commercial bank Limited by Share Ltd. Lugu branch Pledgor: HUNAN TAIGU BIOTECHNOLOGY Co.,Ltd. Registration number: 2017430000054 |
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Date of cancellation: 20180706 Granted publication date: 20140709 Pledgee: Changsha rural commercial bank Limited by Share Ltd. Lugu branch Pledgor: HUNAN TAIGU BIOTECHNOLOGY Co.,Ltd. Registration number: 2017430000054 |
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Address after: 410000 Hunan Changsha national hi tech Industrial Development Zone, 27 Yuan Lu Yu Yuan Pioneer Building 1506, 1507 Patentee after: TAIGU BIOTECHNOLOGY GROUP Co.,Ltd. Address before: 410205 A 14 14, Lulong Road, Changsha, Hunan. Patentee before: HUNAN TAIGU BIOTECHNOLOGY Co.,Ltd. |
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Denomination of invention: Active amino acid water-soluble fertilizer as well as preparation method and application thereof Effective date of registration: 20180813 Granted publication date: 20140709 Pledgee: Changsha bank Limited by Share Ltd. Xiangjiang New District sub branch Pledgor: TAIGU BIOTECHNOLOGY GROUP Co.,Ltd. Registration number: 2018430000056 |
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