CN101544511B - Biological compound fertilizer - Google Patents

Biological compound fertilizer Download PDF

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Publication number
CN101544511B
CN101544511B CN2009100669170A CN200910066917A CN101544511B CN 101544511 B CN101544511 B CN 101544511B CN 2009100669170 A CN2009100669170 A CN 2009100669170A CN 200910066917 A CN200910066917 A CN 200910066917A CN 101544511 B CN101544511 B CN 101544511B
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fertilizer
soil
composite bio
compound fertilizer
biological compound
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CN101544511A (en
Inventor
赵英
刘淑霞
魏汉莲
董蕊
陈春宇
崔耀友
张明远
崔永柱
王义平
王英来
夏瑞明
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Jilin Agricultural University
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Jilin Agricultural University
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Abstract

The invention relates to a biological compound fertilizer, and belongs to the field of agricultural technology. The biological compound fertilizer is used for relieving the problems of environment pollution and ecological balance damage caused by applying excessive fertilizer, and complex process or higher cost in the prior biological compound fertilizer. The biological compound fertilizer comprises the following components mixed in portion by weight: 0.1 to 0.3 portion of compound amino acid and 0.7 to 0.9 portion of biochemical fulvic acid. The biological compound fertilizer is used for soil application and leaf application of crops, can improve physicochemical property of soil, can increase soil fertility, can improve the yield of the crops, and has the advantages of reasonable components, no environment pollution and good effect.

Description

Composite bio-fertilizer
Technical field:
The invention belongs to agricultural technology field, relate to a kind of composite bio-fertilizer.
Background technology:
But surge along with population, for satisfying grain demand, China's fertilizer amount is the trend of sustainable growth, uses chemical fertilizer and makes a great contribution to solving the food shortage problem, but, also brought serious environmental to pollute and to ecological balance damage simultaneously owing to use chemical fertilizer in a large number for a long time.Studies show that the excessive nitrogenous fertilizer of using constantly accumulates in soil, after acquiring a certain degree, can cause that soil environment quality worsens, cause soil compaction, sclerosis, fertility to descend, simultaneously biology, water body, air and HUMAN HEALTH are produced harm.For example, excessive ammonium chloride, ammonium sulfate, Nitrates chemical fertilizer and the urea etc. used can cause the mass consumption of the Soil Nitrate accumulation and the soil organism, influence soil ecosystem, change the biotic population structure, species diversity reduces, and quality of agricultural product descends.Acid ion in the fertilizer residues in soil and makes soil acidification, contains a large amount of phenol in the ammoniacal liquor, apply the farmland after, can cause the phenol of soil to pollute, make agricultural-food produce peculiar smell; The phosphate fertilizer consumption is too much, and harmful minor component wherein, enters and accumulates in human body by diet for after crop absorbs as cadmium, strontium, fluorine, uranium, thorium, lead, mercury etc., and people's health is worked the mischief.For example, cadmium can cause bone common fault and osteoporosis, and fluorine causes tooth fluorine pinta and bone fluorosis etc.For this reason, the pollution by chemical fertilizer problem is all paid much attention in countries in the world at present, emphasizes to want rational use of chemical fertilizer, applying organic manure, development bio-fertilizer.
Along with the development of China's green agriculture, the application in agriculture production of bio-fertilizer or composite bio-fertilizer more and more is subject to people's attention.Retrieval shows, the patented technology of more existing composite bio-fertilizer aspects at present as application number 90101735.3,96109854.6,98121860.1,200710016897.7 etc., but all has complex process or cost problem of higher.The present invention passes through field test, aminoacids complex and biochemical fulvic acid are mixed and made into composite bio-fertilizer, have that technology is simple, cost is low and the good advantage of effect of increasing production, simultaneously can also improve soil physico-chemical property, increase soil fertility, improve crop yield, improve crop quality, reduce environmental pollution, can alleviate owing to excessive and use the problems referred to above that chemical fertilizer produces
Summary of the invention:
The object of the invention provides a kind of composite bio-fertilizer, is used to alleviate owing to excessively use environmental pollution that chemical fertilizer produces and to ecological balance damage, and the problems referred to above of existing of existing composite bio-fertilizer.
Composite bio-fertilizer of the present invention, its technical characterictic are mixed by aminoacids complex 0.1~0.3 weight part and biochemical fulvic acid 0.7~0.9 weight part and form.
The effect of each component among the present invention:
1, aminoacids complex.Aminoacids complex be with natural keratoprotein and vegetable-protein be raw material through hydrochloric acid hydrolysis, form through processes such as decolouring, depickling, desalination and spraying dryings again.The compounded amino acid product that uses among the present invention is the yellowish white powder, and is water-soluble 100%, and moisture absorption is arranged, pH4.2, and nontoxic, odorless is very easily absorbed by crop.Aminoacids complex contains 18 kinds of total free aminoacidss, total amino acid content 51.83% (Threonine 3.89%, aspartic acid 3.68%, Serine 4.75%, L-glutamic acid 9.48%, proline(Pro) 8.54%, glycine 2.73%, L-Ala 1.98%, Gelucystine 1.48%, Xie Ansuan 2.53%, methionine(Met) 0.57%, Isoleucine 1.70%, leucine 0.96%, tyrosine 0.37%, phenylalanine 0.95%, Methionin 1.87%, Histidine 2.30%, arginine 4.01%, tryptophane 0.04%), total nitrogen 22.3% (inorganic nitrogen and organonitrogen).
Amino acid is the basic substance of life, is the important nutrient that animals and plants need jointly.Studies show that, use amino acid plant-growth is had the effect of long-acting and fugitive benefit nitrogen, can strengthen crop disease-resistant, drought resisting and cold tolerance.The little fertile use range of amino acid is extensive, waits all crops all to have the effect that improves output, improves quality to food crop (paddy rice, wheat, corn), oil crops (rape, soybean, peanut), cash crop (cotton, tealeaves, tobacco leaf, mulberry leaf), fruit (apple, pears, citrus, lichee, longan, peach, Lee, apricot, grape) and vegetables (cucumber, green vegetables, French beans).
2, biochemical fulvic acid.Biochemical fulvic acid is a kind of yellow humic acids, is the natural organic substance that extensively is present in the element of the first species's complexity in peat and the weathered coal.Relative molecular weight 680~1450, pure product are buff powder, fineness<120 orders, contain xanthohumic acid>65%, NPK and middle micro-summation>15%, amino acid>6%, water-insoluble<3%, moisture content<8%, pH7~8, contain biochemical activity material and plant endogenous materials such as polysaccharide, polypeptide, multienzyme, nucleic acid, bionic acid, salicylic acid in addition, higher biological activity and multi-function action are arranged.Acid and alkali-resistance, anti-divalent ion, water-soluble fabulous, resistance to hard water is strong, can with the no flocculation sediment in the composite back of various chemical fertilizer, also can be used as soil improvement agent as host, additive, binding agent or the synergistic agent of various fertilizer, to people, animal safety, non-environmental-pollution.
Therefore, by the composite bio-fertilizer that aminoacids complex and biochemical fulvic acid are mixed and made into, be a kind of high reactivity, high-effect, non-harmful green ecological agricultural prods, can not work the mischief to crop and environment.
Preparing composite bio-fertilizer of the present invention only needs 2 kinds of raw materials → measure → mix (3 times) → finished product packing to get final product, and technology is simple.
Use range of the present invention:
The present invention can be used for soil application and the leaf dressing of genseng, the West and other perennial medicinal plant, field crop, fruit tree, vegetables, flowers, herbage etc.Effect the best on perennial plant, reason mainly be since perennial plant at a local continuous growth, after sowing or the transplanting, early growth is good, later stage is because edaphic condition progressively worsens, fertility descends, plant strain growth is slow, disease is serious, cause quality of medicinal material reduction, output to descend, so applying biological composite fertilizer effect is more obvious.
The test effect of the present invention in culture of ginseng:
Test site is that woods ginseng field is liked in the Huinan County, and the genseng kind is " Da-maya ";
1 materials and methods
1) for examination soil
For examination soil is dark brown earth, soil agrochemistry proterties: organic 102.8gkg -1, alkali-hydrolyzable nitrogen 364.3mgkg -1, rapid available phosphorus 23.6mgkg -1, available potassium 245.8mgkg -1, pH6.3.
2) test materials
Aminoacids complex 0.2 weight part and biochemical fulvic acid 0.8 weight part mix the composite bio-fertilizer of forming.
3) test is handled and method
Soil application is handled: a. check plot, do not use composite bio-fertilizer, and per hectare is used the corn tangerine bar that 3000kg pulverizes, and mixes with the dark ginseng bed soil of 30cm; B. the composite bio-fertilizer district used composite bio-fertilizer on April 15th, 2006, and per hectare 30kg composite bio-fertilizer and 3000kg pulverize tangerine bar mixing, are sprinkling upon soil surface equably, turned over, mixed with the dark ginseng bed soil of 30cm for 3 times.
Leaf dressing is handled: a. check plot, do not spray composite bio-fertilizer, and spray clear water in 2006~2008 years exhibition leaf of people latter stages, green fruit phase and red ripe later stage of fruit, water consumption is calculated by per hectare 500Kg in every sub-district; B. composite bio-fertilizer district, earlier with the less water dissolving, the per kilogram composite bio-fertilizer adds water to 250kg to composite bio-fertilizer then, carries out foliage-spray in 2006~2008 years exhibition leaf of people latter stages, green fruit phase and red ripe later stage of fruit, 3 years totally 9 times, fluid volume is calculated by per hectare 500Kg in each sub-district.
Analytical procedure: the soil microorganisms analytical procedure is a plate dilution method, urase phenol-sodium-hypochlorite process, catalase volumetry, saccharase be with 3,5-dinitrobenzene salicylic acid colorimetry, Phosphoric acid esterase is with improved Hoffman method, cellulase anthrone colorimetry, the soil organism are measured and are adopted heavy chromium potassium capacity method, and the soil alkali-hydrolyzable nitrogen adopts the diffusion absorption process, rapid available phosphorus adopts the olsen method, and available potassium adopts flame photometry.
" two, three " cultivation system is adopted in test, promptly uses transplantation of seedlings in 2 years 3 years.3 repetitions, sub-district area 50m are established in test 2, random alignment.Experimental field the gradient is 12.4 °, ploughs degree of depth 30cm, and on October 26th, 2006 transplanted, and tiltedly planted formula.Ginseng bedside 165cm, high 25cm, line-spacing 20cm, spacing in the rows 10cm.Every row 10 strains, 500 strains of every sub-district, other cultivation management measure requires to carry out routinely, and on October 17th, 2008 results are surveyed and are produced, and gather pedotheque simultaneously and analyze.
2, test-results
The effect of soil application composite bio-fertilizer:
1) to the influence of soil microbiology characteristic.The bacterial count in check plot and composite bio-fertilizer district is respectively 46.26 * 10 5, 51.47 * 10 5, the fungi number is respectively 107.25 * 10 4, 131.56 * 10 4, the actinomycetes number is respectively 131.54 * 10 4, 161.39 * 10 4, illustrate and use that bacterium, fungi and the actinomycetes number average in the soil is significantly increased behind the composite bio-fertilizer.The increase of microbial biomass helps lend some impetus to the microbial transformation of organic matter decomposition, mineral compound and improves biological activity of soil.
2) to the influence of soil enzyme activities.The urase content in check plot and composite bio-fertilizer district is respectively 8.86,11.35mg/100g, catalase is respectively 2.23 and 2.84mL/g, saccharase is respectively 19.38 and 27.12mg/g, Phosphoric acid esterase is respectively 2.81 and 3.72mg/g, cellulase is respectively 0.73 and 0.96mg/10g, illustrate use composite bio-fertilizer after soil enzyme activities all be significantly improved.Urase is the important enzyme in the soil nitrogen circulation in the soil; Catalase is important redox enzymes, but the decomposition of its enzymatic hydrogen peroxide helps preventing his toxic action to organism; Saccharase and cellulase participate in the carbon cycle of soil, are a kind of important lytic enzymes that characterizes biological activity of soil; Phosphoric acid esterase then participates in the conversion of soil organophosphorus.The raising of enzymic activity helps lend some impetus to organic conversion.
3) to the influence of soil physical property.After using composite bio-fertilizer, the total cell size of soil contrast increases by 21.2 percentage points, and venting quality increases by 11.3 percentage points, and unit weight reduces 14.5%; In the soil>and the water-stable granule total amount of 0.25mm obviously increases, and wherein (2~0.5mm) reach 77.4%, increase by 15.9 percentage points to the grade of genseng growth most worthy.
4) to the influence of soil organic matter content.The organic content in check plot and composite bio-fertilizer district is respectively 86.35 and 97.65gkg -1Illustrate and use after the composite bio-fertilizer that soil organic matter content is significantly increased.The increase of organic content helps the structure of improving the soil, and promotes crumb structure to form, and improves soil physical properties, strengthens the soil fertilizer performance.
5) to the influence of soil chemistry proterties.The soil alkali-hydrolyzable nitrogen in check plot and composite bio-fertilizer district is respectively 263.0,314.2mgkg -1, rapid available phosphorus is respectively 18.8 and 21.2mgkg -1, available potassium is respectively 216.2,235.9mgkg -1Illustrate and use composite bio-fertilizer, can promote soil with organic matter to decompose, plant nutrients such as nitrogen, phosphorus, potassium are discharged, make various nutritive elements become the state that plant can directly utilize, thereby increased the readily available nutrient of soil.The increase of available nutrient in the soil helps to improve soil supplying nutrient capability, promotes plant-growth.
6) to the influence of genseng disease incidence.After using composite bio-fertilizer, the disease incidence obviously reduces, and the incidence of eqpidemic disease, sclerotium disease and rust rot is respectively 8.3%, 7.2% and 6.4%, and contrast reduces by 63.5%, 86.7% and 76.3% respectively.
7) to the influence (table 1) of genseng output.After using composite bio-fertilizer, genseng output contrast increases by 20.5%, and difference reaches extremely significantly standard.
Table 1 soil application composite bio-fertilizer is to the influence of genseng output
Figure G2009100669170D00051
Annotate: compare with control group, *P<0.01
The effect of foliage-spray composite bio-fertilizer:
Foliage-spray composite bio-fertilizer, genseng output contrast increase by 20.9%, and difference reaches extremely significantly standard.
Table 2 foliage-spray composite bio-fertilizer is to the influence of genseng output
Figure G2009100669170D00052
Annotate: compare with control group, *P<0.01
3, conclusion
Test-results of the present invention shows, uses composite bio-fertilizer and can significantly improve biological activity of soil, soil enzyme activities, soil physics and chemical property, and genseng disease incidence significantly reduces, and the output utmost point significantly improves.
The test effect of the present invention in soybean culture:
Test site is Jilin Agriculture University experiment base, and soybean varieties is " Ji educates 47 ", is tested in 2008 years and carries out.
1 materials and methods
1) for examination soil
For examination soil is black earth, soil agrochemistry proterties: organic 25.4gkg -1, full nitrogen 1.215gkg -1, alkali-hydrolyzable nitrogen 126.2mgkg -1, rapid available phosphorus 18.6mgkg -1, available potassium 181.3mgkg -1, pH6.46.
2) test materials
Aminoacids complex 0.2 weight part and biochemical fulvic acid 0.8 weight part mix the composite bio-fertilizer of forming.
3) test is handled and method
Soil application is handled: a. contrast, do not use composite bio-fertilizer, and use the corn tangerine bar that 3000kg pulverizes, make base fertilizer with full dose deep placement of sowing; B. composite bio-fertilizer district, per hectare 30kg composite bio-fertilizer and 3000kg pulverize tangerine bar mixing, make base fertilizer with full dose deep placement of sowing.
Leaf dressing is handled: a. check plot, do not spray composite bio-fertilizer, and respectively spray clear water one time in initial bloom stage and fruiting period, water consumption is calculated by per hectare 400Kg in every sub-district; B. composite bio-fertilizer district, with the less water dissolving, the per kilogram composite bio-fertilizer adds water to 250kg to composite bio-fertilizer then, respectively sprays once in initial bloom stage and fruiting period earlier, and fluid volume is calculated by per hectare 400Kg in each sub-district, and other is undertaken by production field normal management.
Test is adopted and to be distinguished group of methods at random, repeat for 3 times, 8 row districts, the long 50m of row, line width 0.67m, double row sowing on the mechanical ridge, April 26 ridging, sowing on May 2, gather in the crops every sub-district when ripe in the middle of 6 row convert output.
2, test-results
Behind the soil application composite bio-fertilizer, soybean yields contrast increases by 13.98%; Foliage-spray composite bio-fertilizer, output contrast increase by 13.21%, and difference all reaches remarkable standard.
Table 3 soil application composite bio-fertilizer is to the influence (2008.09.25) of soybean yields
Figure G2009100669170D00061
Annotate: compare with control group, *P<0.01
Table 4 foliage-spray composite bio-fertilizer is to the influence (2008.09.25) of soybean yields
Figure G2009100669170D00062
Annotate: compare with control group, *P<0.01
3, conclusion
Test-results of the present invention shows, no matter be that soil application composite bio-fertilizer or foliage-spray composite bio-fertilizer all can significantly improve soybean yields.
Embodiment:
1, the preparation of composite bio-fertilizer
Get aminoacids complex 20kg and biochemical fulvic acid 80kg,, be finished product above-mentioned 2 kinds of raw materials → measure → mix (3 times) → packing.
2, use for example
Application process in culture of ginseng: during soil application, per hectare 30kg composite bio-fertilizer and 3000kg pulverize tangerine bar (carrier) mixing, are sprinkling upon the ginseng bed soil surface equably, apply in the 30cm deep plough level soil in conjunction with ploughing then, turn over for 3 times, mix with the dark ginseng bed soil of 30cm; When doing the foliage fertilizer use, earlier with the less water dissolving, the per kilogram composite bio-fertilizer adds water to 250kg to composite bio-fertilizer then, respectively sprays once in the exhibition leaf of people latter stage, green fruit phase and red ripe later stage of fruit, per hectare fluid volume 500Kg, other is undertaken by production field normal management.
Application process in soybean culture: during soil application, per hectare 30kg composite bio-fertilizer and 3000kg pulverize tangerine bar mixing, make base fertilizer with full dose deep placement of sowing, sow soybean then; When doing the foliage fertilizer use, with the less water dissolving, the per kilogram composite bio-fertilizer adds water to 250kg to composite bio-fertilizer then earlier, respectively spray once in initial bloom stage and fruiting period, and per hectare fluid volume 400Kg, other is undertaken by production field normal management.
During the foliage-spray composite bio-fertilizer, carry out in the time of should be with the humid air between the lights, if rainfall within back 6 hours of the spray must be resprayed after it is fine 1 time, foliage fertilizer should be with joining with usefulness.

Claims (1)

1. a composite bio-fertilizer is characterized in that: mixed by aminoacids complex 0.1~0.3 weight part and biochemical fulvic acid 0.7~0.9 weight part and form.
CN2009100669170A 2009-05-07 2009-05-07 Biological compound fertilizer Expired - Fee Related CN101544511B (en)

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Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101880202B (en) * 2010-06-25 2013-03-06 郭云征 Additive for improving husbandry yield
CN102173941B (en) * 2011-02-11 2013-05-01 吉林农业大学 Ginseng controlled-release fertilizer and preparation method thereof
CN103880480A (en) * 2014-01-15 2014-06-25 华侨大学 Special amino-acid organic nutrient fertilizer for tieguanyin tea plants and preparation method thereof
CN104557281A (en) * 2014-12-24 2015-04-29 福建佳玛驰生态科技有限公司 Efficient biological complex fertilizer
CN105272747A (en) * 2015-10-30 2016-01-27 广西金神农生态农业科技开发有限公司 Fertilizer special for citrus reticulata
CN106431754A (en) * 2016-09-20 2017-02-22 吉林农业大学 Soil bioactivator
CN106431769A (en) * 2016-10-26 2017-02-22 秦皇岛五弦维爱科技开发有限公司 Fertilizer synergist containing amino acid and preparation method of fertilizer synergist

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CN1830917A (en) * 2006-03-15 2006-09-13 郭云征 Method. for preparing quick-acting compound organic fertilizer
CN101165011A (en) * 2006-10-16 2008-04-23 陕西汉唐环保农业有限公司 Organic potassium fertilizer prepared from composite organic acid and producing method thereof
CN101343201A (en) * 2008-06-27 2009-01-14 贺州学院 Foliage fertilizer for white mulberry and preparation thereof

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