CN104058879A - Preparation method of compound amino acid cheated nano-selenium leaf fertilizer - Google Patents
Preparation method of compound amino acid cheated nano-selenium leaf fertilizer Download PDFInfo
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Abstract
The invention relates to a preparation method of a compound amino acid cheated nano-selenium leaf fertilizer. The preparation method comprises the following steps: (1) performing enzymatic hydrolysis on blood cell protein powder by using compound enzyme; (2) adding 3wt%-5wt% of sugar alcohol into enzymatic hydrolysis liquid, and reacting at 50-70 DEG C for 1-2 hours to obtain reaction liquid; (3) adding nano-selenium, and reacting at 45-65 DEG C for 1-2 hours to obtain initial liquid; (4) adding 5wt%-6wt% of nano titanium dioxide into the initial liquid, reacting at 45-55 DEG C for 0.5-1 hours, heating to 90-95 DEG C, and keeping the temperature for 10-20 minutes to form a final product. The leaf fertilizer prepared by adopting the method is rich in high-activity oligopeptide and free amino acid, easy to absorb by crops and quick to transport in the crops, and selenium and sugar alcohol can be quickly and completely dissolved; the problem of difficult absorption of the leaf fertilizer is solved, the growth speed of the crops is greatly increased, the disease resistance of the crops is greatly improved, and the quality of the crops is improved.
Description
Technical field
The present invention relates to the preparation field of foliage fertilizer, specifically a kind of preparation method of composite aminoacid chelating nanometer selenium foliage fertilizer.
Background technology
Foliage fertilizer is commonly used to supplement fast plant desired nutritional in agricultural, convenient, fast because of it, and the good absorbing effect that root applies fertilizer relatively, less wastage, and the foliage fertilizer of various nutritional types or functional type progressively comes into the market in recent years.But most foliage fertilizer effective contents that recent year is produced are low, it is single to act on, and majority is to be crop trace element supplement, and nutritive ingredient is single.The patent No. is that the Chinese patent of CN101891520 discloses a kind of amino acid foliage fertilizer on August 29th, 2012, by following material by mass percentage, made: 30%-50% plant amino acid, 1%-5% sodium nitrophenolate, 15%-25% urea, 5%-10% ferrous sulfate, 5%-10% borax, 3%-5% copper sulfate, 0.5%-1% ammonium molybdate, 1%-2% manganous sulfate, 4%-8% zinc sulfate, 10%-20% organosilicon and excess water, this foliage fertilizer has excited the vigor of enzyme in crop, improved the photosynthetic efficiency of crop, accelerate its inner nutrition absorption and running, increase chlorophyll content, improve stem substance accumulation and sugar part content, improve crop quality, strengthen crop drought resistance, disease-resistant, degeneration-resistant and immunizing power etc. all has positive effect, but also Shortcomings, for example, single multiple element is mixed and made into concentrated solution, be watered use, element utilization ratio is not high, effect is stable not, after absorption, in making object, conveying efficiency is low etc.
Summary of the invention
The preparation method who the object of this invention is to provide a kind of composite aminoacid chelating nanometer selenium foliage fertilizer.
In order to achieve the above object, the present invention adopts following technical scheme: a kind of preparation method of composite aminoacid chelating nanometer selenium foliage fertilizer, comprises the following steps: (1) obtains enzymolysis solution by globulin powder with complex enzyme zymohydrolysis; (2) to adding weight ratio in described enzymolysis solution, be the sugar alcohol of 3-5%, under 50 ~ 70 ℃ of conditions, react 1 ~ 2h, obtain reaction solution; Described sugar alcohol is that mannitol, Sorbitol Powder, Xylitol and the third trisugar alcohol mix resulting mixing sugar alcohol; (3) in described reaction solution, add nanometer selenium, at 45 ~ 65 ℃, react 1 ~ 2h, obtain just liquid; (4) in described just liquid, add the described just nano titanium oxide of liquid weight 5-6%, at 45 ~ 55 ℃, react 0.5 ~ 1h, be then warming up to 90 ~ 95 ℃ of insulation 10 ~ 20min, form finished product.
By above-mentioned preparation method:
1. globulin powder protein content is abundant, through complex enzyme zymohydrolysis, obtain micromolecular amino acid, obtain being easy to the necessary amino acid of plant absorbed by crops, improve fertilising object disease resistance, improve the function of fertilising crop quality, amino acid, as the adsorption medium of nanometer selenium, guarantees the assimilation effect of nanometer selenium simultaneously.
2. sugar alcohol molecular weight is low, be easy to be absorbed by blade, as photosynthetic product, sugar alcohol is uniquely can carry the material that nutrient transports in phloem, mobile fast in making object, can rest rapidly minute to be transported to needs position, and sugar alcohol itself also has trophic function, can improve the anti-ability of coercing of crop.In technical scheme, under disclosed condition, amino acid and sugar alcohol joint efficiency are high, in conjunction with after can stable existence.
3. selenium can promote to do the raising of object endoperoxide enzymic activity, strengthens the resistance of oxidation of crop, improves its resistance and anti-ageing ability, improves crop yield, improves the quality of crop.Selenium in soil is crop main source, but crop is subject to multifactorial impact to the absorption of selenium, and assimilation effect is poor, and conventional selenate and selenite be due to the similarity with vitriol, sulphite, the be absorbed with Competition of sulphur to selenium.The present invention adopts sugar alcohol and amino acid as soft-medium, can solve it and in making object, absorbs and transport difficult problem.
4. nano titanium oxide has sterilization and disinfection effect, because foliage fertilizer of the present invention includes gas chromatography, easily microbiological contamination, and most of antibacterial be organic substance, they are widely used in food, washing composition, textiles and makeup.But volatile, easy decomposition that they exist produces the shortcomings such as nuisance, security be poor, nano titanium oxide can overcome these defects, and nano titanium oxide is under condition of the present invention, can also improve the viscosity of finished product, be convenient to foliage fertilizer and be adsorbed on blade face, improve its utilization ratio.
As preferably, the ratio of mixture of described mannitol, Sorbitol Powder, Xylitol and the third trisugar alcohol is 1 ~ 3:2 ~ 5:1 ~ 3:1 ~ 3.
These sugar alcohols have higher stability to acid, heat, and the mixing sugar alcohol complexing action being obtained by mixing in the ratio in technical scheme is strong, stable with amino acid effect, and the foliage fertilizer that obtains band is being easier to absorb and doing the transportation in object.
As preferably, described nanometer selenium is for to be mixed with gained by Sodium Selenite and citric acid; The mixed weight ratio of described Sodium Selenite and citric acid is 5 ~ 10:10 ~ 20.
As preferably, described nanometer selenium is for being mixed with gained by sodium lauryl sulphate or polyvinyl alcohol or Xylo-Mucine, Sodium Selenite and citric acid; The massfraction of sodium lauryl sulphate described in reaction system or polyvinyl alcohol or Xylo-Mucine is 1 ~ 5%, and Sodium Selenite is 0.02 ~ 0.1mol/L, and described citric acid is 0.1 ~ 0.5mol/L.
The nano granules of selenium that utilizes sodium lauryl sulphate or polyvinyl alcohol or Xylo-Mucine to make as template is even, and granularity is less, and physico-chemical property is stable, is beneficial to next step reaction.
As preferably, described nanometer selenium is for to be mixed with gained by water-soluble chitosan, Sodium Selenite and citric acid; The mixed weight ratio of described chitosan, Sodium Selenite and citric acid is 100 ~ 150:5:10 ~ 20.
Chitosan is a kind of straight chain polymer polymkeric substance, there is good bio-compatibility, nanometer selenium and the chitosan with biological significance are combined, the selenium of nanomorphic is rolled into stable particle by chitosan absorption, at the chelating amino acids with high biological activity, there is good suspension, emulsification, stabilization, effect that can well stabilized nanoscale selenium, its activity is high, easily absorption, and the quality of crop is had to remarkable efficacy.Chitosan or a good slow-released carrier, can progressively discharge selenium, and it is lasting for selenium effect to play.
As preferably, described prozyme is for being pancreatin, papoid and food flavor enzyme, and enzymatic hydrolysis condition is 50-58 ℃, pH8.0-8.5, enzymolysis 4.5-5.5 hour.
Because the kinds of protein of globulin powder is many, single lytic enzyme hydrolysis is insufficient, utilizes prozyme to make globulin powder hydrolysis more abundant, and described pancreatin is the mixture of trypsinase, pancreatic amylase, steapsase, its function has diversity, and hydrolysis effect is good; In proteolysis process, the N-terminal exposure of some bitter peptides can make hydrolyzed solution with bitter taste, affects its mouthfeel, and food flavor enzyme can be eliminated bitter taste.
As preferably, the mass mixing ratio of described pancreatin, papoid and food flavor enzyme is 1-3:1-2:0.5-1.
Proportioning in this technical scheme is beneficial to the abundant hydrolysis of protein powder, the reaction of can mutually promoting between lytic enzyme, and the peptide chain obtaining is short, is easy to absorb.
As preferably, step (4) is described middlely accounts for the described just bio-surfactant of liquid weight 0.01-0.06% to also having added in described just liquid, and described bio-surfactant is glycolipid class tensio-active agent or fat peptid-based surfactant.
Bio-surfactant has good solubilising, emulsification, reduction surfactivity effect, reaction is had no side effect, add the rear combination to nano titanium oxide and sugar alcohol more stable, can also under the effect of nano titanium oxide, further increase the viscosity of product, be beneficial to adhering to of foliage fertilizer.
As preferably, described bio-surfactant is fat peptid-based surfactant, and described fat peptid-based surfactant is Surfactin class or iturin or bacillomycin.
As preferably, described in step (3), in chelatropic reaction, also added sequestrant, described sequestrant is one or more in citric acid, oxysuccinic acid and lactic acid.
By implementing above-mentioned technical approach, the present invention has following beneficial effect: the foliage fertilizer obtaining by the present invention is rich in highly active oligopeptides and total free aminoacids, and selenium and sugar alcohol, dissolve rapidly thoroughly, crop easily absorbs, also can in making object, transport fast, has solved foliage fertilizer, especially containing the difficult problem absorbing of the organic foliage fertilizers such as amino acid, greatly improve the speed of growth of crop, improve the disease resistance of fertilising crop, improve crop quality.
Embodiment
Embodiment 1:
(1) in enzymolysis is filled with, proteinic powder of porcine be take to mass volume ratio as 1:5 dissolves with pure water, fully mix, be warming up to 50 °, the pancreatin that is 3:1:0.5 by volume ratio, papoid and food flavor enzyme carry out enzymolysis, obtain enzymolysis solution; The pH8.0 of solution during enzymolysis, enzymolysis after 4.5 hours rises to the temperature of enzymatic vessel 90 ° of enzymes that go out and processes, and enzyme 30min goes out; (2) in described enzymolysis solution, add the sugar alcohol that accounts for enzymolysis solution gross weight 3%, under 50 ℃ of conditions, react 1h, then cool the temperature to 20 ° of insulation 10min, obtain reaction solution; Described sugar alcohol is that ratio of mixture is the sugar alcohol mixtures of mannitol, Sorbitol Powder, Xylitol and the third trisugar alcohol of 1:5:3:1; (3) to the citric acid that adds nanometer selenium in described reaction solution and account for reaction solution gross weight 0.3% as sequestrant, the add-on of described nanometer selenium needs the size of selenium content to determine according to prepared foliage fertilizer, the present embodiment is prepared the foliage fertilizer that selenium content is 0.035mol/L, through chelatropic reaction, obtain chelating liquid, the condition of described chelatropic reaction is 45 ℃, chelating 2h, and described nanometer selenium is by weight being mixed with gained for 100:5:10 by water-soluble chitosan, Sodium Selenite and citric acid; (4) in described chelating liquid, add the nano titanium oxide of described chelating liquid weight 5% and 0.01% glycolipid class tensio-active agent, described fat peptid-based surfactant is Surfactin class, at 45 ℃, react 1h, then be warming up to 90 ℃ of insulation 20min, form finished product, concentrated obtain foliage fertilizer, during use according to the growing state of crop reality be watered use vegetative period.
Embodiment 2:
(1) in enzymolysis is filled with, ox or sheep globulin powder be take to mass volume ratio as 1:7 dissolves with pure water, fully mix, be warming up to 65 °, the pancreatin that is 2:1.5:0.75 by volume ratio, papoid and food flavor enzyme carry out enzymolysis, obtain enzymolysis solution; The pH8.5 of solution during enzymolysis, enzymolysis after 5.5 hours rises to the temperature of enzymatic vessel 95 ° of enzymes that go out and processes, and enzyme 20min goes out; (2) in described enzymolysis solution, add the sugar alcohol that accounts for enzymolysis solution gross weight 5%, under 70 ℃ of conditions, react 1h, then cool the temperature to 30 ° of insulation 10min, obtain reaction solution; Described sugar alcohol is that ratio of mixture is the sugar alcohol mixtures of mannitol, Sorbitol Powder, Xylitol and the third trisugar alcohol of 3:2:1:3; (3) to the oxysuccinic acid that adds nanometer selenium in described reaction solution and account for reaction solution gross weight 0.1% as sequestrant, the add-on of described nanometer selenium needs the size of selenium content to determine according to prepared foliage fertilizer, the present embodiment is prepared the foliage fertilizer that selenium content is 0.04mol/L, through chelatropic reaction, obtain chelating liquid, the condition of described chelatropic reaction is 65 ℃, chelating 1h, and described nanometer selenium is by weight being mixed with gained for 150:5:20 by water-soluble chitosan, Sodium Selenite and citric acid; (4) in described chelating liquid, add the nano titanium oxide of described chelating liquid weight 6% and 0.06% glycolipid class tensio-active agent, described fat peptid-based surfactant is Surfactin class, at 55 ℃, react 0.5h, then be warming up to 95 ℃ of insulation 10min, form finished product, the concentrated foliage fertilizer that obtains.
Embodiment 3:
(1) in enzymolysis is filled with, chicken or duck globulin powder be take to mass volume ratio as 1:3 dissolves with pure water, fully mix, be warming up to 55 °, the pancreatin that is 1:2:1 by volume ratio, papoid and food flavor enzyme carry out enzymolysis, obtain enzymolysis solution; The pH8.3 of solution during enzymolysis, enzymolysis after 5 hours rises to the temperature of enzymatic vessel 90 ° of enzymes that go out and processes, and enzyme 20min goes out; (2) in described enzymolysis solution, add the sugar alcohol that accounts for enzymolysis solution gross weight 4%, under 60 ℃ of conditions, react 1.5h, then cool the temperature to 30 ° of insulation 20min, obtain reaction solution; Described sugar alcohol is that ratio of mixture is the sugar alcohol mixtures of mannitol, Sorbitol Powder, Xylitol and the third trisugar alcohol of 2:3:2:2; (3) to add nanometer selenium in described reaction solution and account for the oxysuccinic acid of reaction solution gross weight 0.1% and 0.05% lactic acid as sequestrant, the add-on of described nanometer selenium needs the size of selenium content to determine according to prepared foliage fertilizer, the present embodiment is prepared the foliage fertilizer that selenium content is 0.03mol/L, through chelatropic reaction, obtain chelating liquid, the condition of described chelatropic reaction is 50 ℃, chelating 1.5h, and described nanometer selenium is by weight being mixed with gained for 120:5:15 by water-soluble chitosan, Sodium Selenite and citric acid; (4) in described chelating liquid, add the nano titanium oxide of described chelating liquid weight 6% and 0.06% glycolipid class tensio-active agent, described fat peptid-based surfactant is Surfactin class, at 55 ℃, react 0.5h, then be warming up to 95 ℃ of insulation 10min, form finished product, the concentrated foliage fertilizer that obtains.
Embodiment 4:
(1) in enzymolysis is filled with, chicken or duck globulin powder be take to mass volume ratio as 1:3 dissolves with pure water, fully mix, be warming up to 55 °, the pancreatin that is 1:2:1 by volume ratio, papoid and food flavor enzyme carry out enzymolysis, obtain enzymolysis solution; The pH8.3 of solution during enzymolysis, enzymolysis after 5 hours rises to the temperature of enzymatic vessel 90 ° of enzymes that go out and processes, and enzyme 20min goes out; (2) in described enzymolysis solution, add the sugar alcohol that accounts for enzymolysis solution gross weight 4%, under 60 ℃ of conditions, react 1.5h, then cool the temperature to 30 ° of insulation 20min, obtain reaction solution; Described sugar alcohol is that ratio of mixture is the sugar alcohol mixtures of mannitol, Sorbitol Powder, Xylitol and the third trisugar alcohol of 2:3:2:2; (3) to add nanometer selenium in described reaction solution and account for the oxysuccinic acid of reaction solution gross weight 0.1% and 0.05% lactic acid as sequestrant, the add-on of described nanometer selenium needs the size of selenium content to determine according to prepared foliage fertilizer, the present embodiment is prepared the foliage fertilizer that selenium content is 0.03mol/L, through chelatropic reaction, obtain chelating liquid, the condition of described chelatropic reaction is 50 ℃, chelating 1.5h, and described nanometer selenium is that Sodium Selenite and citric acid are by weight being mixed with gained for 5:15; (4) in described chelating liquid, add the nano titanium oxide of described chelating liquid weight 6% and 0.06% glycolipid class tensio-active agent, described fat peptid-based surfactant is Surfactin class, at 55 ℃, react 0.5h, then be warming up to 95 ℃ of insulation 10min, form finished product, the concentrated foliage fertilizer that obtains.
Embodiment 5:
(1) in enzymolysis is filled with, ox or sheep globulin powder be take to mass volume ratio as 1:7 dissolves with pure water, fully mix, be warming up to 65 °, the pancreatin that is 2:1.5:0.75 by volume ratio, papoid and food flavor enzyme carry out enzymolysis, obtain enzymolysis solution; The pH8.5 of solution during enzymolysis, enzymolysis after 5.5 hours rises to the temperature of enzymatic vessel 95 ° of enzymes that go out and processes, and enzyme 20min goes out; (2) in described enzymolysis solution, add the sugar alcohol that accounts for enzymolysis solution gross weight 5%, under 70 ℃ of conditions, react 1h, then cool the temperature to 30 ° of insulation 10min, obtain reaction solution; Described sugar alcohol is that ratio of mixture is the sugar alcohol mixtures of mannitol, Sorbitol Powder, Xylitol and the third trisugar alcohol of 3:2:1:3; (3) to the oxysuccinic acid that adds nanometer selenium in described reaction solution and account for reaction solution gross weight 0.1% as sequestrant, the add-on of described nanometer selenium needs the size of selenium content to determine according to prepared foliage fertilizer, the present embodiment is prepared the foliage fertilizer that selenium content is 0.04mol/L, through chelatropic reaction, obtain chelating liquid, the condition of described chelatropic reaction is 65 ℃, chelating 1h, and described nanometer selenium is for being mixed with gained by sodium lauryl sulphate or polyvinyl alcohol or Xylo-Mucine, Sodium Selenite and citric acid; The present embodiment is Xylo-Mucine, and described in reaction system, the massfraction of Xylo-Mucine is 3%, and Sodium Selenite is 0.05mol/L, and described citric acid is 0.3mol/L; (4) in described chelating liquid, add the nano titanium oxide of described chelating liquid weight 6% and 0.06% glycolipid class tensio-active agent, described fat peptid-based surfactant is Surfactin class, at 55 ℃, react 0.5h, then be warming up to 95 ℃ of insulation 10min, form finished product, the concentrated foliage fertilizer that obtains.
Comparative example 1:
(1) in enzymolysis is filled with, proteinic powder of porcine be take to mass volume ratio as 1:5 dissolves with pure water, fully mix, be warming up to 50 °, the pancreatin that is 3:1:0.5 by volume ratio, papoid and food flavor enzyme carry out enzymolysis, obtain enzymolysis solution; The pH8.0 of solution during enzymolysis, enzymolysis after 4.5 hours rises to the temperature of enzymatic vessel 90 ° of enzymes that go out and processes, and enzyme 30min goes out; (2) in described enzymolysis solution, add the sugar alcohol that accounts for enzymolysis solution gross weight 0.1%, under 85 ℃ of conditions, react 1h, obtain reaction solution; Described sugar alcohol is that ratio of mixture is the sugar alcohol mixtures of mannitol, Sorbitol Powder, Xylitol and the third trisugar alcohol of 1:5:3:1; (3) to the citric acid that adds nanometer selenium in described reaction solution and account for reaction solution gross weight 0.3% as sequestrant, the add-on of described nanometer selenium needs the size of selenium content to determine according to prepared foliage fertilizer, the present embodiment is prepared the foliage fertilizer that selenium content is 0.035mol/L, through chelatropic reaction, obtain chelating liquid, the condition of described chelatropic reaction is 45 ℃, chelating 2h, and described nanometer selenium is by weight being mixed with gained for 100:5:10 by water-soluble chitosan, Sodium Selenite and citric acid; (4) in described chelating liquid, add the nano titanium oxide of described chelating liquid weight 5% and 0.01% glycolipid class tensio-active agent, described fat peptid-based surfactant is Surfactin class, at 45 ℃, react 1h, then be warming up to 90 ℃ of insulation 20min, form finished product, concentrated obtain foliage fertilizer, during use according to the growing state of crop reality be watered use vegetative period.
Comparative example 2:
(1) in enzymolysis is filled with, proteinic powder of porcine be take to mass volume ratio as 1:5 dissolves with pure water, fully mix, be warming up to 50 °, the pancreatin that is 3:1:0.5 by volume ratio, papoid and food flavor enzyme carry out enzymolysis, obtain enzymolysis solution; The pH8.0 of solution during enzymolysis, enzymolysis after 4.5 hours rises to the temperature of enzymatic vessel 90 ° of enzymes that go out and processes, and enzyme 30min goes out; (2) in described enzymolysis solution, add the sugar alcohol that accounts for enzymolysis solution gross weight 3%, under 50 ℃ of conditions, react 1h, then cool the temperature to 20 ° of insulation 10min, obtain reaction solution; Described sugar alcohol is that ratio of mixture is the sugar alcohol mixtures of mannitol and the third trisugar alcohol of 1:1; (3) to the citric acid that adds nanometer selenium in described reaction solution and account for reaction solution gross weight 0.3% as sequestrant, the add-on of described nanometer selenium needs the size of selenium content to determine according to prepared foliage fertilizer, the present embodiment is prepared the foliage fertilizer that selenium content is 0.035mol/L, through chelatropic reaction, obtain chelating liquid, the condition of described chelatropic reaction is 45 ℃, chelating 2h, and described nanometer selenium is by weight being mixed with gained for 100:5:10 by water-soluble chitosan, Sodium Selenite and citric acid; (4) in described chelating liquid, add the nano titanium oxide of described chelating liquid weight 5% and 0.01% glycolipid class tensio-active agent, described fat peptid-based surfactant is Surfactin class, at 45 ℃, react 1h, then be warming up to 90 ℃ of insulation 20min, form finished product, concentrated obtain foliage fertilizer, during use according to the growing state of crop reality be watered use vegetative period.
Comparative example 3: as different from Example 1, plus nano titanium dioxide not in step (4).
Comparative example 4: as different from Example 1, chelatropic reaction condition is 80 °, chelating 1h.
Comparative example 5: as different from Example 1, the reaction conditions in step (4) is 69 ° of reaction 1h, is not incubated processing.
Comparative example 6: produce a kind of selenium-enriched foliage fertilizer product:
A, respectively sodium selenate 0.01%, Sodium Selenite 0.01% are dissolved in 1 kilogram of distilled water, slowly add yellow humic acid 0.5%, and constantly stir, obtain lysate, standby;
B, by 50 kg water, sodium nitrophenolate 1.0% is dissolved again, add urea 5.0% and sodium laurylsulfonate 0.5%, stir to dissolve and mix, obtain lysate;
C, again step a and step b two portions lysate are mixed after, add ammoniacal liquor to regulate pH to 6.5, supply moisture, obtain product.
Comparative example 7: prepare 1 ton of amino acid foliage fertilizer: take 500kg plant amino acid, 150kg urea, 50kg ferrous sulfate, 50kg borax, 30kg copper sulfate, 5kg ammonium molybdate, 10kg manganous sulfate, 40kg zinc sulfate, water fully mixes after dissolving in reactor respectively, after again plant modifying agent 10kg DA-6,10kg sodium nitrophenolate and 100kg organosilicon being weighed, add in reactor and stir at normal temperatures, finally add excess water.
Above-described embodiment and the resulting foliage fertilizer of comparative example are carried out to functional verification test, subjects is little epipremnum aureum, green vegetables, wheat, respectively select essentially identical 110 strains of upgrowth situation in vegetative period, be respectively divided into 11 groups, every group of 10 strains, be placed under isometric growth condition, spray respectively the foliage fertilizer of embodiment and comparative example, weekly, spray continuously 2 months, the time of spraying is dusk on same day 3-5 point, and control group sprays clear water.Result is as table 1:
Table 1:
The wherein I of phenotype, II, III, IV level, respectively representative: I: plant is sturdy, riotous growth, resistant to lodging, blade is emerald green, without insect pest; II: plant is relatively sturdy, growth is very fast, blade shows slightly yellow, substantially without insect pest; III: plant is short and small, growth is slow, have Huang Ye, easily lodging, damaged by vermin; IV: plant is thin and weak, short and small, yellow blade is more, and whole strain blade is partially yellow, and here most plant wither, insect pest is more.
From the above results, can find out, spray the plant of foliage fertilizer of embodiment 1-3 gained compared with comparative example 1-7 and control group, plant strain growth is fast, disease and insect resistance is strong, and overall quality is good.
To using the edible part of the crop obtaining after the resulting foliage fertilizer of above-described embodiment and comparative example, carry out the mensuration of selenium content, subjects is wheat, paddy rice, tomato, cucumber, green vegetables, spinach, in the vegetative period of heading stage, tomato and cucumber result initial stage, green vegetables and the spinach of wheat and paddy rice, respectively select essentially identical 110 strains of corresponding period and upgrowth situation, respectively be divided into 11 groups, every group of 10 strains, it is sprayed to foliage fertilizer, once in a week, spray continuously 2 months, the time of spraying is dusk on same day 3-5 point, and control group sprays clear water.When edible, get the edible position of its correspondence, survey its selenium content, (mg/kg) averages.Result is as table 2:
Table 2:
By the above results, the resulting foliage fertilizer of embodiment, compared with comparative example and control group, is beneficial to the absorption of selenium and the transportation in making object, has greatly improved the selenium content of crop, is conducive to eater's supplement selenium element.
Claims (8)
1. a preparation method for composite aminoacid chelating nanometer selenium foliage fertilizer, is characterized in that: comprise the following steps: (1) obtains enzymolysis solution by globulin powder with complex enzyme zymohydrolysis; (2) to adding weight ratio in described enzymolysis solution, be the sugar alcohol of 3-5%, under 50 ~ 70 ℃ of conditions, react 1 ~ 2h, obtain reaction solution; Described sugar alcohol is that mannitol, Sorbitol Powder, Xylitol and the third trisugar alcohol mix resulting mixing sugar alcohol; (3) in described reaction solution, add nanometer selenium, at 45 ~ 65 ℃, react 1 ~ 2h, obtain just liquid; (4) in described just liquid, add the described just nano titanium oxide of liquid weight 5-6%, at 45 ~ 55 ℃, react 0.5 ~ 1h, be then warming up to 90 ~ 95 ℃ of insulation 10 ~ 20min, form finished product.
2. a kind of preparation method of composite aminoacid chelating nanometer selenium foliage fertilizer according to claim 1, is characterized in that: the ratio of mixture of described mannitol, Sorbitol Powder, Xylitol and the third trisugar alcohol is 1 ~ 3:2 ~ 5:1 ~ 3:1 ~ 3.
3. a kind of preparation method of composite aminoacid chelating nanometer selenium foliage fertilizer according to claim 1, is characterized in that: described nanometer selenium is for to be mixed with gained by Sodium Selenite and citric acid; The mixed weight ratio of described Sodium Selenite and citric acid is 5 ~ 10:10 ~ 20.
4. a kind of preparation method of composite aminoacid chelating nanometer selenium foliage fertilizer according to claim 1, is characterized in that: described prozyme is pancreatin, papoid and food flavor enzyme, and enzymatic hydrolysis condition is 50-58 ℃, pH8.0-8.5, enzymolysis 4.5-5.5 hour.
5. a kind of preparation method of composite aminoacid chelating nanometer selenium foliage fertilizer according to claim 4, is characterized in that: the mass mixing ratio of described pancreatin, papoid and food flavor enzyme is 1-3:1-2:0.5-1.
6. a kind of preparation method of composite aminoacid chelating nanometer selenium foliage fertilizer according to claim 1, it is characterized in that: step (4) is described middlely accounts for the described just bio-surfactant of liquid weight 0.01-0.06% to also having added in described just liquid, and described bio-surfactant is glycolipid class tensio-active agent or fat peptid-based surfactant.
7. a kind of preparation method of composite aminoacid chelating nanometer selenium foliage fertilizer according to claim 6, it is characterized in that: described bio-surfactant is fat peptid-based surfactant, described fat peptid-based surfactant is Surfactin class or iturin or bacillomycin.
8. a kind of preparation method of composite aminoacid chelating nanometer selenium foliage fertilizer according to claim 1, is characterized in that: described in step (3), in reaction, also added sequestrant, described sequestrant is one or more in citric acid, oxysuccinic acid and lactic acid.
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