CN112806370A - Agricultural composition containing 5-aminolevulinic acid and dihydrojasmonic acid propyl ester - Google Patents
Agricultural composition containing 5-aminolevulinic acid and dihydrojasmonic acid propyl ester Download PDFInfo
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- CN112806370A CN112806370A CN202110142871.7A CN202110142871A CN112806370A CN 112806370 A CN112806370 A CN 112806370A CN 202110142871 A CN202110142871 A CN 202110142871A CN 112806370 A CN112806370 A CN 112806370A
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- propyl ester
- agricultural composition
- acid
- dihydrojasmonic
- aminolevulinic acid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
Abstract
The invention provides an agricultural composition containing 5-aminolevulinic acid and dihydrojasmonic acid propyl ester, which comprises active ingredients playing a regulating role on plants, wherein the active ingredients comprise the 5-aminolevulinic acid and the dihydrojasmonic acid propyl ester, and the mass ratio of the 5-aminolevulinic acid to the dihydrojasmonic acid propyl ester in the composition is 80: 1-1: 10. The agricultural composition can improve the quality of plant fruits, promote the coloring of the plant fruits, and promote the germination of crop seeds and the growth of seedlings.
Description
Technical Field
The invention belongs to the field of agricultural compositions, relates to a plant growth regulating composition, and particularly relates to a plant growth regulating composition containing 5-aminolevulinic acid and dihydrojasmonic acid propyl ester and application thereof.
Background
The plant growth regulator is divided into two main categories of natural extraction and artificial synthesis, has the growth and development regulating effect similar to that of natural plant hormone, is an organic compound which is artificially synthesized and has similar physiological and biological effects with the plant hormone after people know the structure and the action mechanism of the natural plant hormone, and is used in agricultural production to effectively regulate the growth process of crops, thereby achieving the purposes of stabilizing yield, increasing production, improving quality, enhancing stress resistance of the crops and the like.
5-aminolevulinic acid (ALA) is a substance widely present in natural animals and plants, and is a synthetic precursor of porphyrins (chlorophyll, heme, phytochrome, and the like). 5-aminolevulinic acid is involved in biochemical reactions in many organisms, such as photosynthesis, vitamin B12 and heme synthesis, among others. In recent years, the research on 5-aminolevulinic acid and derivatives (salts and esters) thereof has been further advanced and widely applied. It has been found that ALA is not simply a biological metabolic intermediate, but is also involved in the regulation of plant growth and development.
Dihydrojasmonate (PDJ) is an artificially synthesized jasmonic acid derivative with high biological activity. Compared with Jasmonic Acid (JA) and methyl jasmonate (JA-Me), PDJ has the characteristics of good chemical stability, low volatility and longer duration of physiological effect. At low concentrations, PDJ has a stronger promoting effect than JA and is considered to be a jasmonic compound with more practical value.
Disclosure of Invention
The invention aims to provide an agricultural composition capable of effectively increasing the fruit coloring rate and improving the fruit quality, and relates to a plant growth regulating composition containing 5-aminolevulinic acid and dihydrojasmonic acid propyl ester.
Specifically, the plant growth regulating composition containing 5-aminolevulinic acid and dihydro jasmonic acid propyl ester provided by the invention comprises active ingredients playing a regulating role on plants, wherein the active ingredients comprise the 5-aminolevulinic acid and the dihydro jasmonic acid propyl ester, and the mass ratio of the 5-aminolevulinic acid to the dihydro jasmonic acid propyl ester in the plant growth regulating composition is 80: 1-1: 10.
It is easily known to those skilled in the art that the combined use or combined use of a plurality of plant growth regulators (pesticides) is one of the leading trends of the future agrochemical market, but in the case of plant growth regulator (pesticide) compositions, the mechanism and mechanism of each component are unknown, it is unpredictable whether the synergistic effect between the active ingredients of the plant growth regulators (pesticides) can be produced. According to the evaluation of comprehensive effects shown by field tests, the plant growth regulator provided by the invention has the advantages that the quality-improving and color-increasing effects on fruits such as grapes and apples are better than the effects of single agent, the plant growth regulator is safe, and no phytotoxicity is generated. In addition, according to further experimental research of the inventor, the composition can increase the area and thickness of plant leaves and increase the content of chlorophyll when being used for cultivating garden plants; when the fertilizer is used for coating crop seeds, the seed germination and seedling growth can be promoted.
Further, the invention provides a plant growth regulating composition containing 5-aminolevulinic acid and dihydrojasmonic acid propyl ester, wherein the mass ratio of the 5-aminolevulinic acid to the dihydrojasmonic acid propyl ester in the plant growth regulating composition is 30: 1-1: 1.
In order to fully exert the efficacy of the active ingredient of the plant growth regulating composition (pesticide), it may be prepared into a formulation form convenient for application according to the use requirement. Preferably, the preparation formulation of the composition is soluble powder, water dispersible granules, wettable powder, soluble liquid, dispersible oil suspending agent and suspending agent. In formulating the various formulations described above, it will be apparent to those skilled in the art that a variety of adjuvants may be required in addition to the optional plant growth regulating active ingredient.
The auxiliary agents include but are not limited to wetting and dispersing agents, film forming agents, thickening agents, coloring agents, preservatives, antifoaming agents, antifreezing agents and other auxiliary components which are allowed to be used in pesticides, and are all components which are commonly used or allowed to be used in pesticide preparations or plant growth regulators.
The invention also provides the application of the plant growth regulating composition in the aspects of improving the fruit quality of plants and promoting the fruit coloring of the plants. As mentioned above, the composition has excellent effects of improving quality and increasing color of plant fruits such as grapes, apples and the like within a proper dosage ratio range. The plant growth regulator and the preparation thereof have various application modes, the invention is not particularly limited, and one or a combination of various application modes such as spraying, root irrigation, root soaking, seed soaking, spreading, furrow application, hole application and the like can be adopted. Generally, according to the conventional application method of the exogenous plant growth regulator, the agricultural composition for promoting the fruit quality improvement and color enhancement is generally sprayed in the expansion period and the coloring period of the plant fruits.
In addition, the invention also provides application of the plant growth regulating composition in the aspects of improving the germination rate of crops and improving the growth condition of seedlings. Within a proper dosage concentration range, the composition can effectively promote the germination of seeds and the growth of seedlings of crops such as corn, wheat, soybean and the like. Generally, in the seed treatment aspect, the plant growth regulating composition is used as a seed coating agent to dip-coat crop seeds.
Compared with the prior art, the plant growth regulating composition has the following beneficial effects or advantages: the invention provides a plant growth regulating composition taking 5-aminolevulinic acid and dihydrojasmonic acid propyl ester as active ingredients, and through the intensive research of the inventor, the plant growth regulating composition can improve the quality of plant fruits, promote the coloring of the plant fruits, promote the germination of crop seeds and the growth of seedlings and increase the leaf area and the thickness of garden plants in a certain concentration range.
Detailed Description
The following examples are given to illustrate the technical aspects of the present invention, but the present invention is not limited to the following examples.
Example 1
This example explores the hyperchromic effect of a plant growth regulating composition containing 5-aminolevulinic acid and propyl dihydrojasmonate on grapes. The experiment selection is carried out in the experimental base of the Weifang city agricultural academy of sciences, the experimental crop is 3-year-old red Balaiti grape, and the normal water and fertilizer management is unified.
5-aminolevulinic acid and dihydrojasmonic acid propyl ester are compounded into a mixed treatment medicament according to the proportion shown in the table 1, and 5-aminolevulinic acid, dihydrojasmonic acid propyl ester and clear water are used as single agentsThe same additives were added to the control treatment to achieve a better control. Each dosage form is respectively provided with 750 times, 1000 times and 1250 times of treatment, and each treatment area is 30m2And the communities are randomly distributed, the grapes are sprayed 2-4 weeks before harvest in the color conversion period, the grapes are uniformly sprayed to the whole tree to achieve the purpose of dropping liquid, and each treatment is repeated for 4 times.
Investigating character indexes after fruit ripening: the coloring degree is randomly investigated for 10 clusters, each cluster randomly picks 10 typical fruit grains, and the coloring area ratio of the standard color of the fruit surface is determined; the content of soluble solids is randomly investigated for 10 clusters, each cluster is randomly picked for 10 typical fruit grains, and the typical fruit grains are measured by a saccharimeter (digital refractometer); the yield of 5 normal bearing trees was investigated, and the average value was calculated and multiplied by the number of cultivated plants per unit area to calculate the yield per unit area. The data recording results are shown in table 1.
TABLE 1 influence of ALA-PDJ composition on the quality and yield of red baradi grapes
As can be seen from the data in Table 1, the active ingredients of the plant growth regulating composition containing 5-aminolevulinic acid and dihydrojasmonic acid propyl ester have different degrees of influence on the quality and the yield of grapes within the mass ratio range of 80: 1-1: 10, and compared with a single agent, the plant growth regulating composition has more obvious effects of increasing the color, the quality and the yield. Further, when the compounding ratio is 30: 1-1: 1, the effect is most obvious, the degree of coloration reaches 35% at most, and is improved by 20% compared with a blank control; the yield reaches 1199.23 kg/mu to the maximum, and is increased by 14.4 percent compared with a blank control.
Example 2
This example explores the effect of a plant growth regulating composition containing 5-aminolevulinic acid and propyl dihydrojasmonate on the quality and color enhancement of apples. The test is carried out on a certain apple planting base in Shaanxi province, Xiyangyang city, the variety is 10-year-old red Fuji fruit trees, the red Fuji fruit trees are respectively provided with a bagged fruit tree area and an un-bagged fruit tree area, and the areas are uniformly and normally subjected to water and fertilizer management.
5-aminolevulinic acid and dihydrojasmonic acid propyl ester (the mass ratio of the 5-aminolevulinic acid to the dihydrojasmonic acid propyl ester in the mixed medicament is 30:1) are taken as mixed treatment medicaments, a single 5-aminolevulinic acid, the dihydrojasmonic acid propyl ester and clear water are taken as comparison treatment medicaments, and the same auxiliary agents are added to achieve a better comparison effect. Each dosage form was provided with a concentration gradient of 150mg/L, 350mg/L, 550mg/L, respectively.
The fruit bag is removed from the bagged fruit 25 days before picking, and the fruit is soaked with the above medicinal liquid. The fruit is directly soaked in the above medicinal liquid for 30 times (3 times) for each treatment.
When the fruit reached maturity, harvesting was started and from each iteration, 5 fruits were selected, i.e. 15 fruits per treatment, and the rind (4 pieces per fruit, approximately 0.5mm thick) was randomly peeled from the fruit carcass for a total of 60 pieces. After mixing, evenly weighing the mixture in three groups, and then measuring the content of anthocyanin on the surface of the fruit by a colorimetric method. The flesh portion was squeezed into a juice and the soluble solids content was measured with a hand-held refractometer.
Pulp endogenous ALA assay: taking a certain amount of pulp tissue, grinding and extracting by using acetic acid buffer solution with pH4.6, centrifuging, taking 1mL of supernate, adding a certain amount of acetylacetone, condensing at 100 ℃ for 10min, cooling, adding 1mL of color development liquid, developing for 5-10min, carrying out color comparison at 553nm of an ultraviolet visible spectrophotometer, and making a standard curve by using ALA standard reagent.
All the above measurements were repeated 3 times or more, and averaged, and the data of the results are reported in tables 2 and 3.
TABLE 2 Effect of ALA-PDJ exogenous treatment on coloration and quality of unpackaged apples
TABLE 3 Effect of ALA-PDJ exogenous treatment on coloring and quality of bagged apples
As is well known to those skilled in the art, anthocyanins are water-soluble natural pigments in plants, and the coloration of fruits can be judged according to the anthocyanin content of the pericarp. The data in tables 2 and 3 show that ALA-PDJ exogenous treatment obviously improves the anthocyanin content (based on fresh weight) of apple peels, and shows better coloring effect even at 550 mg/L.
From the soluble solid content, the combined use of ALA and PDJ and the single dosage of ALA and PDJ are higher than the control group (CK) in different degrees no matter whether the ALA and PDJ are bagged or not, and compared with the single dosage, the combined use of the ALA and PDJ has slightly higher soluble solid content, which indicates that the combined use of the ALA and the PDJ has the synergistic effect.
From the view of the content of the pulp endogenous ALA, the endogenous ALA of the fruit without bags is generally higher than that of the fruit with bags, which is supposed to be related to the fact that the ALA synthesis is promoted by the long-term illumination condition of the fruit with bags. In addition, when the ALA-PDJ composition and the single dosage of ALA and PDJ are used, the increase of endogenous ALA in the pulp is not very large compared with that of a blank control, and the substances are proved to have very little residue in apples, and are safe and harmless.
Example 3
This example explores the effect of a plant growth regulating composition comprising 5-aminolevulinic acid and propyl dihydrojasmonate on crop seed germination.
The inventor prepares 61% of pesticide seed suspending agent containing 5-aminolevulinic acid and dihydrojasmonic acid propyl ester, and the pesticide seed suspending agent comprises the following components in percentage by weight: 60% of 5-aminolevulinic acid, 1% of propyl dihydrojasmonate, 3% of sodium lauryl sulfate serving as a wetting agent, 4% of sodium naphthalenesulfonate formaldehyde condensate serving as a dispersant, 5% of ethylene glycol serving as an antifreeze, 0.2% of xanthan gum serving as a thickening agent, 0.8% of rose essence serving as a warning color and sterile water for supplementing to 100%.
The indoor bioassay test is carried out by respectively taking corn, wheat and barley seeds as test objects. The variety is the most commonly used seed at present and is not treated by any medicament. After sun seed selection of each test variety, soaking seeds for 8 hours by using 1000 times of the pesticide seed suspending agent respectively, sowing the seeds into a culture dish paved with wet filter paper, accelerating germination in a constant-temperature incubator at 28 +/-1 ℃, sowing the seeds on a plastic box stretched with a gauze after 2 days, and culturing seedlings (containing tap water in the box) to investigate the germination rate. Culturing with nutrient solution after 4 days, and culturing in light culture room with light irradiation of 12 hr each day at day temperature of 30 + -1 deg.C and night temperature of 25 + -1 deg.C. After the seedlings grow for 10 days, sampling and investigating physiological indexes of the seedlings, respectively weighing the drying weights of roots and crowns, repeating each treatment for 4 times, and recording result data shown in table 4.
TABLE 4 Effect of 61% ALA PDJ seed suspension infusion on crop seed Germination
As can be seen from Table 4, the germination percentage of the seeds can be remarkably improved by soaking the seeds with the ALA-PDJ seed suspending agent by about 10-13%. As can be seen from the growth indexes of the seedlings, the root length, the dry weight of the crown, the dry weight of the root and the ratio of the root to the crown of each treatment are obviously greater than those of the control, which shows that the seed suspending agent provided by the invention improves the root activity of the seedlings and can effectively promote the growth of the seedlings.
As described above, the present invention can be preferably implemented, and the above-mentioned embodiments only describe the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various changes and modifications of the technical solution of the present invention made by those skilled in the art without departing from the design spirit of the present invention shall fall within the protection scope defined by the present invention.
Claims (6)
1. An agricultural composition containing 5-aminolevulinic acid and dihydrojasmonic acid propyl ester is characterized in that active ingredients playing a role in regulating plants are composed of the 5-aminolevulinic acid and the dihydrojasmonic acid propyl ester, wherein the mass ratio of the 5-aminolevulinic acid to the dihydrojasmonic acid propyl ester in the agricultural composition is 80: 1-1: 10.
2. An agricultural composition according to claim 1, wherein the mass ratio of the 5-aminolevulinic acid to the dihydrojasmonic acid propyl ester in the composition is 30: 1-1: 1.
3. Use of the agricultural composition of claim 1 or 2 for improving fruit quality and promoting fruit coloring.
4. The use according to claim 3, wherein the fruits comprise grapes and apples, and the agricultural composition is sprayed during the fruit's enlargement phase and coloring phase.
5. Use of the agricultural composition of claim 1 or 2 for increasing the germination rate of crop seeds and improving the growth of seedlings.
6. The use of claim 5, wherein the crop seeds comprise corn, barley, wheat, and the agricultural composition is formulated as a seed coating, and the seeds are coated by seed soaking.
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| CN202110142871.7A CN112806370A (en) | 2021-02-02 | 2021-02-02 | Agricultural composition containing 5-aminolevulinic acid and dihydrojasmonic acid propyl ester |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113615695A (en) * | 2021-09-17 | 2021-11-09 | 郑州郑氏化工产品有限公司 | Composition for improving fruit tree yield and quality and application thereof |
| CN113841699A (en) * | 2021-10-12 | 2021-12-28 | 鲁东大学 | Composition for promoting coloring and sweetening of grapes |
| CN114521556A (en) * | 2022-01-05 | 2022-05-24 | 天津市汉邦植物保护剂有限责任公司 | Soluble solution containing propyl dihydrojasmonate and chlorogenic acid and preparation method thereof |
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2021
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113615695A (en) * | 2021-09-17 | 2021-11-09 | 郑州郑氏化工产品有限公司 | Composition for improving fruit tree yield and quality and application thereof |
| CN113841699A (en) * | 2021-10-12 | 2021-12-28 | 鲁东大学 | Composition for promoting coloring and sweetening of grapes |
| CN114521556A (en) * | 2022-01-05 | 2022-05-24 | 天津市汉邦植物保护剂有限责任公司 | Soluble solution containing propyl dihydrojasmonate and chlorogenic acid and preparation method thereof |
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