CN106962402A - The inducer of inducing wheat anti gibberellic disease - Google Patents
The inducer of inducing wheat anti gibberellic disease Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/24—Cyanogen or compounds thereof, e.g. hydrogen cyanide, cyanic acid, cyanamide, thiocyanic acid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/12—Iodine, e.g. iodophors; Compounds thereof
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Abstract
The invention discloses a kind of inducer for luring anti-wheat anti gibberellic disease, the component of the inducer of the wheat anti gibberellic disease includes sodium nitroprussiate, salicylic acid, chitosan oligosaccharide, KI, per 100ml inducers in the parts by weight of each component be：The sodium nitroprussiate 0.75g of mass concentration 99.5%~1.5g, the salicylic acid 3g of mass concentration 99.5%~7.5g, the 1.5~3g of chitosan oligosaccharide of mass concentration 95%, the KI 1.0g of mass concentration 99.9%~1.5g, wherein salicylic acid ethanol dissolves, other to be dissolved with water.Inducer of the present invention is sprayed in wheat leaf blade in wheat beginning ear period, after being absorbed by blade, the activity of activation wheat activity in vivo material and enzyme, and resistance gibberellic hypha infects and suppresses Spot expansion, prevention and the generation and harm of reduction wheat scab.
Description
Technical field
The present invention relates to inducer, and in particular to a kind of inducer of inducing wheat anti gibberellic disease, inducing wheat resists red mould
Disease infects, and prevents and reduce the generation of head blight.
Background technology
Cloudy weather for several days running, moist many dew, sultry weather conditions are frequently encountered after wheat heading, will pandemic
Head blight, causes disaster in a short time.
Head blight is to threaten one of Major Diseases of Wheat Production, worldwide there is generation.China is in the world
The maximum country of wheat scab harm, the hm of annual injured area about 7,500,0002, the 1/4 of national wheat area is accounted for, yield is caused
Lose 2,000,000-300 ten thousand t（Simpson D R,Weston G E,Turner J A,et al.Differential control
of head blight pathogens of wheat by fungicides and consequences for
mycotoxin contamination of grain.European Journal of Plant Pathology,2001,107
(4)：421-431 ）.With the change of weather, recent years, head blight did harm to as regular incidence, not only influenceed wheat yield, also shadow
The quality of wheat has been rung, and wheat scab can cause wheat infected seed to produce a variety of toxic substances such as rotten sickle mykol of deoxidation snow
（deoxynival,DON）And zearalenone（zearlenone, ZEA）Deng people and animals are eaten after these infected seeds, it may occur that no
With the toxic reaction of degree, such as allergic and functions of intestines and stomach is disorderly, causes the diseases such as loss of appetite, diarrhoea, vomiting, stomachache
The effect of shape, and retarding of growing, the immunity function of reduction body, also carcinogenic, teratogenesis and mutagenesis, can cause pregnant woman's miscarriage even
It is dead（Rocha O,Ansari K,Doohan F M.Effects of trichothecene mycotoxins on
eukaryotic cells:A review.Food Addit Contam,2005,22(4):369-378）.
In production to the preventing and treating of wheat scab based on chemical pesticide and disease-resistant variety, the extensive application of chemical pesticide is drawn
The drug-fast generation of fusarium graminearum is played, stimulates germ to make a variation, the new microspecies of generation or variation lines make the anti-of wheat scab
Control that work is more and more difficult, the prevention effect of medicine is more and more poor, organic agricultural chemicals dosage is improved constantly, and gives mankind's ring
Border causes huge pollution, and the pollution to environment and food aggravates, and directly affects human health.Disease-resistant variety is due to plant
Hereditary disease resistance trait is difficult to organically combine with other economical characters, and seed selection is not only disease-resistant but also high yield, the difficulty of the kind of high-quality are more next
It is bigger.The amount of labour used for carrying out disease control using traditional agronomic culture measure is big, and prevention effect is also extremely limited.Therefore, solve
Certainly the method for these disadvantages, is always one of significant effort direction of agricultural science and technology worker in world wide.
The research of On Induced Disease Resistance In Plants in recent years is that plant disease effectively preventing and treating brings hope.On Induced Disease Resistance In Plants are again
Claim system resume, be plant under the stimulation or effect of certain biology or abiotic component, have more to pathogen
The feature of strong repellence.On Induced Disease Resistance In Plants are to rise important research field in the recent period in the world, utilize plant inducer impedance disease
Property is considered as the new technology and new way of plant protection.According to Kuc etc.（KUC J, S RICHMOND .Aspects of the
protection of cucumber against Colletotrichum lagernarium by Colletotrichum
lagernarium .Phytopathology , 1977 , 67:533-536）Research show that cucumber topical vaccination tobacco is bad
Dead virus (Tobacco necrosis virus, TNV), bacterial angular leaf spot bacterium (Pseudomonas lanchrymans)
Or the inducible plant of Colletotrichumlagenarium (Celletotrichum lagenarium) produced wide spectrum system in several days and resisted
Property.Biles etc.（BILES C L , MARTYN R D.Local and systemic resistance induced in
Watermelons by formae specials of Fusarium oxysporum.Phytopathology, 1989（79）:
856-860）Fusarium is withered with cucumber sharp fusarium (Fusarium oxysporum f .sp .cucumerinum) nontoxic microspecies
Listless watermelon of the disease with different resistances is inoculated with, and induction generates its part and system resistant to anthrax-bacilus, uses 0.1 g/kg
Acetyl salicylic acid treatment tobacco, find its produce to downy mildew and the resistance of tobacco mosaic virus disease.Anfoka etc.（ANFOKA
G, BUCHENAUER H .Systemic acquired resistance in tomato against Phytophtora
infestans by pre-inoculation with tobacco necrosis virus.Phys Mol Plant Path,
1997, 50 :85-101）With tobacco necrosis virus pre-vaccination tomato induction of to cucumber mosaic virus (Cucumber
Mosaic virus, CMV) strain Y resistance, so as to delay disease development, reduce disease index.Smith etc.
（SMITH J A,METRAUX J P.Pseudomonas syringae patho var.syringae induces
systemic resistance to Pyricularia oryzae in rice .Physiol Mol Plant Path ,
1991, 39:451 -461）In the leaf of paddy rice first Pseudomonas syrzngae pv .syrzngae challenge inoculations, production
The resistance to rice blast fungus is given birth to.Kingdom's equality（Kingdom puts down, and Pan Chuanzhi, Ren Guoxin wait plant source crop inducers red to wheat
The inhibition Agricultural University Of Hunan journal of mildew（Natural science edition）, 2001,27（1）：50-52）Utilize botanical material BZ
Anti gibberellic disease is produced with CF inducing wheats, inhibition respectively reaches 55.41% and 48.5%, effectively the harm of reduction head blight.
Wang Hongtao et al. applications granted patent " a kind of to be used to prevent and treat sustained release agent of wheat scab and preparation method thereof "（The patent No.
zl201410584561.0）It is that, using chitosan oligosaccharide and jinggangmycin A as the compound sterilizing sustained release agent of main component, its active ingredient is pressed
The content of mass percent is 1%~3% chitosan oligosaccharide and 10%~20% jinggangmycin A.Zhao come into et al. apply for a patent " one kind preventing and treating
The microbicide compositions of wheat scab and its application "（Application No. 201410485523.X）It is with metconazole and amino-oligosacchride
Element is the bactericide of main component, and the total content of metconazole and amino oligosaccharide is 5%~60%, metconazole and amino oligosaccharide
Weight ratio is（1：10）~（10:1）." a kind of chitosan oligosaccharide for anti-wheat scab is combined the patent of Zhang Shanxue et al. applications
The purposes and method of thing "（Application No. 201110332967.6）It is that chitosan oligosaccharide is added in composition pesticide, it is in agricultural chemicals group
Percentage by weight 0.003%~0.028% in compound.These patents for containing the materials such as chitosan oligosaccharide, amino-oligosaccharide all do not have but
Mention the material have lure anti gibberellic disease and effect.
The content of the invention
The purpose of the present invention is：A kind of inducer of inducing wheat anti gibberellic disease is provided, uniformly sprayed in wheat beginning ear period
In wheat blade face, the activity of activation wheat activity in vivo material and enzyme resists infecting for gibberellic hypha, suppresses Spot expansion, in advance
Generation that is anti-and reducing head blight, it is to avoid and mitigate harm of the head blight to wheat.
The present invention technical solution be：The component of the inducer of the inducing wheat anti gibberellic disease include sodium nitroprussiate,
The parts by weight of each component are in salicylic acid, chitosan oligosaccharide, KI, every 100ml inducers：The sodium nitroprussiate of mass concentration 99.5%
0.75g~1.5g, the salicylic acid 3g of mass concentration 99.5%~7.5g, the 1.5~3g of chitosan oligosaccharide of mass concentration 95%, mass concentration
99.9% KI 1.0g~1.5g, wherein salicylic acid ethanol dissolves, other to be dissolved with water.
The preparation method of the inducer of above-mentioned inducing wheat anti gibberellic disease comprises the following steps：
（1）Following components is taken respectively according to parts by weight：The sodium nitroprussiate 0.75g of mass concentration 99.5%~1.5g, mass concentration 99.5%
Salicylic acid 3g~7.5g, the chitosan oligosaccharide 1.5g of mass concentration 95%~3g, the KI 1.0g of mass concentration 99.9%~1.5g；
（2）Salicylic acid ethanol is dissolved, dissolving ratio is that the salicylic acid of 1g mass concentrations 99.5% 3ml ethanol dissolves；
（3）Sodium nitroprussiate water is dissolved, dissolving ratio is that the sodium nitroprussiate 10ml of 1g mass concentrations 99.5% dissolves；
（4）Chitosan oligosaccharide is added into sodium nitroprusside solution, and carries out being sufficiently stirred for dissolving；
（5）To step（4）Mixing liquid in add KI, and carry out being sufficiently stirred for dissolving；
（6）By step（2）The salicylic acid ethanol solution of gained adds step（5）Mixing liquid in, stir evenly mixing；
（7）To step（6）Mixed liquor in add a small amount of running water, stir evenly, and be dissolved to 100ml, obtain inducing wheat resist it is red mould
The inducer of disease.
The application method of the inducer of above-mentioned inducing wheat anti gibberellic disease is：In wheat beginning ear period, per 667m2Use 100ml
Inducer is uniformly sprayed to water 30kg, after stirring evenly in wheat leaf blade surface, and every being sprayed again once after 7 days, consumption is identical.
Compared with prior art, the present invention has following obvious advantage：
First, the inducer of inducing wheat anti gibberellic disease of the invention is sprayed in wheat leaf blade in wheat beginning ear period, is absorbed by blade,
Wheat activity in vivo material and the activity of some enzymes are activated, the intrusion of gibberellic hypha is effective against and suppresses the extension of scab, from
And prevent and reduce the generation of head blight, mitigate harm of the head blight to wheat, reduce the usage amount of chemical pesticide, protecting agriculture
Ecological environment and human body health.
2nd, the salicylic acid and chitosan oligosaccharide in the present invention all have the activity of enzyme and active material, resistance in activation wheat body
The intrusion of gibberellic hypha and the effect for suppressing Spot expansion, superposition synergy is compounded by both, is reduced each plant demand, is reduced into
This.
3rd, the sodium nitroprussiate in the present invention is NO foreign donor, and it produces substantial amounts of NO after being absorbed by wheat, NO by with
The iron ion of its soluble recepter guanylate cyclase (guanylatecyclase, GC) is combined, and changes GC stereochemical structure
So as to improve its activity, and further result in the increase of intracellular second messenger's ring cytimidine list nucleoside phosphorylase (cGMP) generation
, the cGMP of formation acts on phenylalanine ammonia lyase (PAL) and adjusts salicylic acid again（SA）Accumulation so that inducing wheat
The expression of the disease-resistant related gene such as PAL and pathogenesis-related proteins (PR-1), and then participate in the disease-resistant of wheat（Head blight）Reaction.
4th, on the one hand the KI in the present invention is made by being absorbed after foliage-spray by wheat leaf blade by osmotic adjustment
With reduction pathogen spore germination；On the other hand improve the content of the calcium ion in wheat body and promote the signals such as salicylic acid point
The dynamic accumulation of son, accelerates the increase of local cell death and cell leakage, signal is further amplified, these signals promote again
The activity of relevant enzyme in the metabolic processes such as active oxygen, aldehydes matter is entered, so as to increase disease-resistant wheat（Head blight）Property.
5th, the inducer of inducing wheat anti gibberellic disease of the invention is sprayed in wheat leaf blade in wheat beginning ear period, and wheat is red
Mildew inhibiting rate is up to more than 37.81%, and the inducer of inducing wheat anti gibberellic disease of the invention has preferable economic benefit.
6th, wheat scab derivant of the invention is the new prevention of a class and reduces the product that head blight occurs, and leads to
Cross and excite plant immune system, reach the purpose of diseases prevention；Itself has no bactericidal activity, but it can excite plant generation system to obtain
Property resistance is obtained, the immunologic mechanism of inside plants is excited, the high yield and high quality potentiality of wheat is not fully exerted, the life of wheat is ensured
Production and edible safety, protection human body health.
Embodiment
With reference to specific embodiment further illustrate the present invention technical solution, these embodiments it is not intended that
It is the limitation to technical scheme.
Embodiment 1：The inducer of inducing wheat anti gibberellic disease is prepared according to following steps
（1）Following components is taken respectively according to parts by weight：The sodium nitroprussiate 0.75g of mass concentration 99.5%, the salicylic acid of mass concentration 99.5%
3.00g, the chitosan oligosaccharide 1.50g of mass concentration 95%, the KI 1.00g of mass concentration 99.9%；
（2）Salicylic acid ethanol is dissolved, dissolving ratio is that the salicylic acid of 1.00g mass concentrations 99.5% 3ml ethanol dissolves；
（3）Sodium nitroprussiate water is dissolved, dissolving ratio is that the sodium nitroprussiate 10ml of 1.00g mass concentrations 99.5% dissolves；
（4）Chitosan oligosaccharide is added into sodium nitroprusside solution, and carries out being sufficiently stirred for dissolving；
（5）To step（4）Mixing liquid in add KI, and carry out being sufficiently stirred for dissolving；
（6）By step（2）The salicylic acid ethanol solution of gained adds step（5）Mixing liquid in, stir evenly mixing；
（7）To step（6）Mixed liquor in add a small amount of running water, stir evenly, and be dissolved to 100ml, obtain inducing wheat resist it is red mould
The inducer of disease.
The application method of the inducer of above-mentioned inducing wheat anti gibberellic disease is：In wheat beginning ear period, per 667m2Use 100ml
Inducer is uniformly sprayed to water 30kg, after stirring evenly in wheat leaf blade surface, and every being sprayed again once after 7 days, consumption is identical.
Tested in Huai'an Agricultural Science and Technology Park within 2015, soil fertility is medium on the upper side, preceding stubble is paddy rice, experiment is set
It is processed as：The inducer of the inducing wheat anti gibberellic disease of the present embodiment 1 sprays processing and compareed（Do not spray inducing wheat resist it is red mould
Sick inducer）, three repeat, and plot area is 20m2, wheat breed is that Huaihe River wheat is sowed on October 25th, 33,2015, line-spacing 25cm,
Field rich water quality management, pest control measure are identical；On April 20th, 2016, foliage-spray once, sprayed once again every 7 days；Harvest
A situation arises for preceding each processing cell head blight of investigation, and per the 5 points of samplings of cell diagonal, every takes 60 fringes, total number of samples 300
Fringe；Severity Scaling investigation is carried out, disease index is counted；Calculate each according to the disease index of processing cell and not dispenser check plot
Handle the relative inhibition to head blight；Severity Scaling standard and disease index computational methods are with reference to GB/T15796-2011
Gibberella zeaze petch of wheat and barley observes and predicts investigation specifications；Severity Scaling：It is 0 grade, disease-free；I grade, sick spikelet number accounts for less than the 1/4 of whole small ears；Ⅱ
Level, sick spikelet number accounts for the 1/4~1/2 of whole small ears；III grade, sick spikelet number accounts for the 1/2~3/4 of whole small ears；IV grade, sick small ear
Number accounts for more than the 3/4 of whole small ears.
Disease index=∑（The sick spike numbers at different levels × number of levels）×100/（Investigate total spike number × 4）
State of an illness inhibiting rate=（Check plot disease index-treatment region disease index）/ check plot disease index
Result of the test shows：The wheat scab inhibiting rate for spraying the inducer of the inducing wheat anti gibberellic disease of the present embodiment 1 reaches
37.81%（It see the table below）.
Embodiment 2：The inducer of inducing wheat anti gibberellic disease is prepared according to following steps
（1）Following components is taken respectively according to parts by weight：The sodium nitroprussiate 1.00g of mass concentration 99.5%, the salicylic acid of mass concentration 99.5%
4.50g, the chitosan oligosaccharide 2.00g of mass concentration 95%, the KI 1.25g of mass concentration 99.9%；
（2）Salicylic acid ethanol is dissolved, dissolving ratio is that the salicylic acid of 1.00g mass concentrations 99.5% 3ml ethanol dissolves；
（3）Sodium nitroprussiate water is dissolved, dissolving ratio is that the sodium nitroprussiate 10ml of 1.00g mass concentrations 99.5% dissolves；
（4）Chitosan oligosaccharide is added into sodium nitroprusside solution, and carries out being sufficiently stirred for dissolving；
（5）To step（4）Mixing liquid in add KI, and carry out being sufficiently stirred for dissolving；
（6）By step（2）The salicylic acid ethanol solution of gained adds step（5）Mixing liquid in, stir evenly mixing；
（7）To step（6）Mixed liquor in add a small amount of running water, stir evenly, and be dissolved to 100ml, obtain inducing wheat resist it is red mould
The inducer of disease.
The application method of the inducer of above-mentioned inducing wheat anti gibberellic disease is：In wheat beginning ear period, per 667m2Use 100ml
Inducer is uniformly sprayed to water 30kg, after stirring evenly in wheat leaf blade surface, and every being sprayed again once after 7 days, consumption is identical.
Tested in Huai'an Agricultural Science and Technology Park within 2015, soil fertility is medium on the upper side, preceding stubble is paddy rice, experiment is set
It is processed as：The inducer of the inducing wheat anti gibberellic disease of the present embodiment 2 sprays processing and compareed（Do not spray inducing wheat resist it is red mould
Sick inducer）, three repeat, and plot area is 20m2, wheat breed is that Huaihe River wheat is sowed on October 25th, 33,2015, line-spacing 25cm,
Field rich water quality management, pest control measure are identical；On April 20th, 2016, foliage-spray once, sprayed once again every 7 days；Harvest
A situation arises for preceding each processing cell head blight of investigation, and per the 5 points of samplings of cell diagonal, every takes 60 fringes, total number of samples 300
Fringe；Severity Scaling investigation is carried out, disease index is counted；Calculated according to each processing cell and the disease index of not dispenser check plot
Relative inhibition of each processing to head blight；Severity Scaling standard and disease index computational methods are with reference to GB/T15796-2011
Gibberella zeaze petch of wheat and barley observes and predicts investigation specifications；Severity Scaling：It is 0 grade, disease-free；I grade, sick spikelet number accounts for less than the 1/4 of whole small ears；Ⅱ
Level, sick spikelet number accounts for the 1/4~1/2 of whole small ears；III grade, sick spikelet number accounts for the 1/2~3/4 of whole small ears；IV grade, sick small ear
Number accounts for more than the 3/4 of whole small ears.
Disease index=∑（The sick spike numbers at different levels × number of levels）×100/（Investigate total spike number × 4）
State of an illness inhibiting rate=（Check plot disease index-treatment region disease index）/ check plot disease index
Result of the test shows：The wheat scab inhibiting rate for spraying the inducer of the inducing wheat anti gibberellic disease of the present embodiment 2 reaches
46.24%（It see the table below）.
Embodiment 3：The inducer of inducing wheat anti gibberellic disease is prepared according to following steps
（1）Following components is taken respectively according to parts by weight：The sodium nitroprussiate 1.25g of mass concentration 99.5%, the salicylic acid of mass concentration 99.5%
6.00g, the chitosan oligosaccharide 3.00g of mass concentration 95%, the KI 1.00g of mass concentration 99.9%；
（2）Salicylic acid ethanol is dissolved, dissolving ratio is that the salicylic acid of 1.00g mass concentrations 99.5% 3ml ethanol dissolves；
（3）Sodium nitroprussiate water is dissolved, dissolving ratio is that the sodium nitroprussiate 10ml of 1.00g mass concentrations 99.5% dissolves；
（4）Chitosan oligosaccharide is added into sodium nitroprusside solution, and carries out being sufficiently stirred for dissolving；
（5）To step（4）Mixing liquid in add KI, and carry out being sufficiently stirred for dissolving；
（6）By step（2）The salicylic acid ethanol solution of gained adds step（5）Mixing liquid in, stir evenly mixing；
（7）To step（6）Mixed liquor in add a small amount of running water, stir evenly, and be dissolved to 100ml, obtain inducing wheat resist it is red mould
The inducer of disease.
The application method of the inducer of above-mentioned inducing wheat anti gibberellic disease is：In wheat beginning ear period, per 667m2Use 100ml
Inducer is uniformly sprayed to water 30kg, after stirring evenly in wheat leaf blade surface, and every being sprayed again once after 7 days, consumption is identical.
Tested in Huai'an Agricultural Science and Technology Park within 2015, soil fertility is medium on the upper side, preceding stubble is paddy rice, experiment is set
It is processed as：The inducer of the inducing wheat anti gibberellic disease of the present embodiment 3 sprays processing and compareed（Do not spray inducing wheat resist it is red mould
Sick inducer）, three repeat, and plot area is 20m2, wheat breed is that Huaihe River wheat is sowed on October 25th, 33,2015, line-spacing 25cm,
Field rich water quality management, pest control measure are identical；On April 20th, 2016, foliage-spray once, sprayed once again every 7 days；Harvest
A situation arises for preceding each processing cell head blight of investigation, and per the 5 points of samplings of cell diagonal, every takes 60 fringes, total number of samples 300
Fringe；Severity Scaling investigation is carried out, disease index is counted；Calculated according to each processing cell and the disease index of not dispenser check plot
Relative inhibition of each processing to head blight；Severity Scaling standard and disease index computational methods are with reference to GB/T15796-2011
Gibberella zeaze petch of wheat and barley observes and predicts investigation specifications；Severity Scaling：It is 0 grade, disease-free；I grade, sick spikelet number accounts for less than the 1/4 of whole small ears；Ⅱ
Level, sick spikelet number accounts for the 1/4~1/2 of whole small ears；III grade, sick spikelet number accounts for the 1/2~3/4 of whole small ears；IV grade, sick small ear
Number accounts for more than the 3/4 of whole small ears.
Disease index=∑（The sick spike numbers at different levels × number of levels）×100/（Investigate total spike number × 4）
State of an illness inhibiting rate=（Check plot disease index-treatment region disease index）/ check plot disease index
Result of the test shows：The wheat scab inhibiting rate for spraying the inducer of the inducing wheat anti gibberellic disease of the present embodiment 3 reaches
49.28%（It see the table below）.
Embodiment 4：The inducer of inducing wheat anti gibberellic disease is prepared according to following steps
（1）Following components is taken respectively according to parts by weight：The sodium nitroprussiate 1.50g of mass concentration 99.5%, the salicylic acid of mass concentration 99.5%
7.50g, the chitosan oligosaccharide 3.00g of mass concentration 95%, the KI 1.50g of mass concentration 99.9%；
（2）Salicylic acid ethanol is dissolved, dissolving ratio is that the salicylic acid of 1.00g mass concentrations 99.5% 3ml ethanol dissolves；
（3）Sodium nitroprussiate water is dissolved, dissolving ratio is that the sodium nitroprussiate 10ml of 1.00g mass concentrations 99.5% dissolves；
（4）Chitosan oligosaccharide is added into sodium nitroprusside solution, and carries out being sufficiently stirred for dissolving；
（5）To step（4）Mixing liquid in add KI, and carry out being sufficiently stirred for dissolving；
（6）By step（2）The salicylic acid ethanol solution of gained adds step（5）Mixing liquid in, stir evenly mixing；
（7）To step（6）Mixed liquor in add a small amount of running water, stir evenly, and be dissolved to 100ml, obtain inducing wheat resist it is red mould
The inducer of disease.
The application method of the inducer of above-mentioned inducing wheat anti gibberellic disease is：In wheat beginning ear period, per 667m2Use 100ml
Inducer is uniformly sprayed to water 30kg, after stirring evenly in wheat leaf blade surface, and every being sprayed again once after 7 days, consumption is identical.
Tested in Huai'an Agricultural Science and Technology Park within 2015, soil fertility is medium on the upper side, preceding stubble is paddy rice, experiment is set
It is processed as：The inducer of the inducing wheat anti gibberellic disease of the present embodiment 4 sprays processing and compareed（Do not spray inducing wheat resist it is red mould
Sick inducer）, three repeat, and plot area is 20m2, wheat breed is that Huaihe River wheat is sowed on October 25th, 33,2015, line-spacing 25cm,
Field rich water quality management, pest control measure are identical；On April 20th, 2016, foliage-spray once, sprayed once again every 7 days；Harvest
A situation arises for preceding each processing cell head blight of investigation, and per the 5 points of samplings of cell diagonal, every takes 60 fringes, sample number total 300
Fringe；Severity Scaling investigation is carried out, disease index is counted；Calculated according to each processing cell and the disease index of not dispenser check plot
Relative inhibition of each processing to head blight；Severity Scaling standard and disease index computational methods are with reference to GB/T15796-2011
Gibberella zeaze petch of wheat and barley observes and predicts investigation specifications；Severity Scaling：It is 0 grade, disease-free；I grade, sick spikelet number accounts for less than the 1/4 of whole small ears；Ⅱ
Level, sick spikelet number accounts for the 1/4~1/2 of whole small ears；III grade, sick spikelet number accounts for the 1/2~3/4 of whole small ears；IV grade, sick small ear
Number accounts for more than the 3/4 of whole small ears.
Disease index=∑（The sick spike numbers at different levels × number of levels）×100/（Investigate total spike number × 4）
State of an illness inhibiting rate=（Check plot disease index-treatment region disease index）/ check plot disease index
Result of the test shows：The wheat scab inhibiting rate for spraying the inducer of the inducing wheat anti gibberellic disease of the present embodiment 4 reaches
54.66%（It see the table below）.Compared to other examples, this example formulations lures the effect of anti-wheat scab best.
Table difference inducer is to wheat scab inhibition
。
Claims (3)
1. the inducer of inducing wheat anti gibberellic disease, it is characterized in that：Wrapped in the component of the inducer of the inducing wheat anti gibberellic disease
Sodium nitroprussiate, salicylic acid, chitosan oligosaccharide, KI are included, the parts by weight of each component are in every 100ml inducers：Mass concentration 99.5%
Sodium nitroprussiate 0.75g~1.5g, the salicylic acid 3g of mass concentration 99.5%~7.5g, the 1.5~3g of chitosan oligosaccharide of mass concentration 95%, quality is dense
99.9% KI 1.0g~1.5g is spent, wherein salicylic acid ethanol dissolves, other to be dissolved with water.
2. the inducer of inducing wheat anti gibberellic disease according to claim 1, it is characterized in that the inducing wheat resist it is red mould
The preparation method of the inducer of disease comprises the following steps：：
（1）Following components is taken respectively according to parts by weight：The sodium nitroprussiate 0.75g of mass concentration 99.5%~1.5g, mass concentration 99.5%
Salicylic acid 3g~7.5g, the chitosan oligosaccharide 1.5g of mass concentration 95%~3g, the KI 1.0g of mass concentration 99.9%~1.5g；
（2）Salicylic acid ethanol is dissolved, dissolving ratio is that the salicylic acid of 1g mass concentrations 99.5% 3ml ethanol dissolves；
（3）Sodium nitroprussiate water is dissolved, dissolving ratio is that the sodium nitroprussiate 10ml of 1g mass concentrations 99.5% dissolves；
（4）Chitosan oligosaccharide is added into sodium nitroprusside solution, and carries out being sufficiently stirred for dissolving；
（5）To step（4）Mixing liquid in add KI, and carry out being sufficiently stirred for dissolving；
（6）By step（2）The salicylic acid ethanol solution of gained adds step（5）Mixing liquid in, stir evenly mixing；
（7）To step（6）Mixed liquor in add a small amount of running water, stir evenly, and be dissolved to 100ml, obtain inducing wheat resist it is red mould
The inducer of disease.
3. the inducer of inducing wheat anti gibberellic disease according to claim 1, it is characterized in that the inducing wheat resist it is red mould
The application method of inducer of disease is：In wheat beginning ear period, per 667m2With 100ml inducers to water 30kg, uniform spray after stirring evenly
Wheat leaf blade surface is imposed on, every being sprayed again once after 7 days, consumption is identical.
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