CN105104389B - Composition and application containing Acibenzolar and chitosan oligosaccharide - Google Patents
Composition and application containing Acibenzolar and chitosan oligosaccharide Download PDFInfo
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- CN105104389B CN105104389B CN201510373601.1A CN201510373601A CN105104389B CN 105104389 B CN105104389 B CN 105104389B CN 201510373601 A CN201510373601 A CN 201510373601A CN 105104389 B CN105104389 B CN 105104389B
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- CGIHPACLZJDCBQ-UHFFFAOYSA-N acibenzolar Chemical compound SC(=O)C1=CC=CC2=C1SN=N2 CGIHPACLZJDCBQ-UHFFFAOYSA-N 0.000 title claims abstract description 25
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Abstract
The invention belongs to plant protection art, it is related to a kind of composition containing Acibenzolar and chitosan oligosaccharide, the weight ratio of Acibenzolar and chitosan oligosaccharide is 1:0.05 10, at least one of spraying and pouring root are used to plant with the composition that content is 5 100mg/L, have excellent preventive effect to the disease of various plants, it is especially obvious to the downy mildew of plant, powdery mildew and rust, sclerotiniose synergy.
Description
Technical field
The invention belongs to plant protection art, more particularly to the composition containing Acibenzolar and chitosan oligosaccharide and application.
Background technology
With the improvement of people ' s living standards and the concern to ecological environment, demand and quality of the people to water fruits and vegetables
Increasingly improve.But increase income how fruit, vegetable increase production as agricultural cultivation industry urgent problem, plant growth regulating
The application of agent can promote plant growth, improve yield and enhancing resistance, be gourd, fruit and vegetable increasing both production and income major measure it
One.Product of the present invention is widely used in multiclass plant culture, such as crop, fruit tree, vegetables, flowers, promotes plant disease-proof, promotes
Growing way and volume increase, the development containing oligosaccharide product are significant to Development of Pollution-free Agricultural Products, Developing Sustainable Agriculture.
Acibenzolar is diazosulfide carboxylic acid esters Plant activator.Acibenzolar English name is ac
Ibenzolar, chemical name are benzo (1,2,3) thiadiazoles -7- carbothioic acid-S- methyl esters, molecular formula C8H6N2OS2, activation
Ester obtains resistance by activating the defense response of crop itself, so that plant produces to a variety of fungies, bacterium, nematode and virus
The self-protection effect of wide spectrum.
Chitosan oligosaccharide leads antiviral agents as a kind of important plant inducer, and from the research of the oligosaccharide of plant, research shows,
Chitosan oligosaccharide can induce during resisting pathogen and corroding and excite plant to produce a series of defense reaction, and these defence are anti-
It should act synergistically and prevent the propagation and invasion of pathogen, have the function that to prevent and cure diseases.It is reported that the derivative of chitosan oligosaccharide also has
There is certain preventive effect.
The composition of Acibenzolar and chitosan oligosaccharide can significantly improve preventive effect and volume increase, can solve current agricultural chemicals single dose application
During resistance and cost the problems such as.
The content of the invention
To improve plant diseases prevention and volume increase, the invention provides the composition containing Acibenzolar and chitosan oligosaccharide and using skill
Art.
Composition containing Acibenzolar and chitosan oligosaccharide, the weight ratio for being characterised by Acibenzolar and chitosan oligosaccharide is 1:0.05-10.It is excellent
Choosing, the weight ratio of chitosan oligosaccharide and Acibenzolar is 1:0.125-8.It is further preferred that the weight of chitosan oligosaccharide and Acibenzolar ratio is
1:0.2-5。
The invention provides the purposes of above-mentioned composition, it is characterised in that the composition of Acibenzolar and chitosan oligosaccharide is used to prevent and treat
Disease, promote volume increase.
The purposes of above-mentioned composition, it is characterised in that the disease is the fungus-caused downy mildew of Mastigomycotina, late blight
Disease, peronospora tabacina etc., the fungus-caused powdery mildew of Ascomycotina, sclerotiniose, head blight, leaf spot etc., Basidiomycotina draws
Rust, the smut etc. risen, the fungus-caused bakanae disease of Fungi Imperfecti, scab etc., wheat rosette stunt, jade caused by virosis
Rice dwarf wilt, black streaked dwarf virus of rice etc..Preferably, the disease is downy mildew, powdery mildew, rust, sclerotiniose.
The invention provides the application process of above-mentioned composition, it is characterised in that the application dose of composition is 5-100mg/
L.Preferably, the application dose of the composition containing Acibenzolar and chitosan oligosaccharide is 10-50mg/L.
The invention provides the application process of above-mentioned composition, it is characterised in that using spraying and fills composition to plant
The processing of at least one of root, the composition identical or different with application concentration first is applied again 0-3 times, combination every 7-20d
The application dose of thing is 5-100mg/L.
The application process of above-mentioned composition, the plant refer to any plant directly or indirectly cultivated for human needs,
Including vegetables, fruits and vegetables, grain and cotton, lawn, medicinal plant, such as potato, tomato, citrus, banana, pears, grape, mango, lichee, dragon
Eye, apple, peach, apricot, Kiwi berry, strawberry, cherry, rape, watermelon, cucumber, sponge gourd, balsam pear, wax gourd, capsicum, pimento, eggplant,
Wheat, rice, corn, tealeaves, cotton etc..Preferably, plant is cucumber, wheat, rape, grape, apple, strawberry, watermelon, silk
Melon, balsam pear, wax gourd.
In the present invention, chitosan oligosaccharide is the high score that the degree of polymerization that Glucosamine is formed by connecting by Isosorbide-5-Nitrae-glycosidic bond is 2-30
The mixture of sub- polymer, mean molecule quantity are 1000-3000 dalton.Chitosan oligosaccharide can be prepared by any means, such as
Following method：Chitosan of the deacetylation more than 70% passes through physical degradation methods, chemical degradation method, enzyme edman degradation Edman, glycosyl
The degree of polymerization that the methods of transfer method, composite degradation method is prepared is the mixture of 2-30 chitosan oligomer.Or by from
Extraction obtains chitin and by physical degradation methods, chemical degradation method, enzyme edman degradation Edman, glycosyl transfer method, multiple in fungal cell wall
The degree of polymerization that the methods of closing edman degradation Edman is prepared is the mixture of 2-30 chitosan oligomer.Or by by aminoglucose
The mixture for the polymer that the degree of polymerization that sugar is polymerized by 1,4- glycosidic bond chemical syntheses is 2-30.Chitosan oligosaccharide is at present
The mixture of the high molecular polymer of different polymer, that is to say, that it is not the oligosaccharides form of the single degree of polymerization in chitosan oligosaccharide,
But the chitosan oligosaccharide monose of every kind of degree of polymerization all there may be.So no matter be prepared by which kind of method, or by upper
The commercially available prod that preparation method is prepared is stated, as long as it is the degree of polymerization that Glucosamine is formed by connecting by Isosorbide-5-Nitrae-glycosidic bond
Mixture for the high molecular polymer that 2-30, mean molecule quantity are 1000-3000 dalton is exactly that shell of the present invention is few
Sugar, it can contain acetyl group, but be bonded amount of the sugar unit no more than 40% of acetyl group, preferably more than 30% amount,
Particularly preferably it is no more than 20% amount.Presently commercially available chitosan oligosaccharide is substantially all the chitosan oligosaccharide illustrated by above-mentioned chitosan oligosaccharide term
Product.
Beneficial effects of the present invention：
Composition containing Acibenzolar and chitosan oligosaccharide diseases prevention, promote plant growing way, significantly reach effect of increasing production.
Embodiment
In order to understand the present invention, the present invention is further illustrated with embodiment below, but be not limited to the present invention.
Prevention effect of the composition of embodiment 1 to cucumber downy mildew
Single compound or the dissolving of composition solvent are prepared into 1000mg/L mother liquor.It is pre- according to biological activity determination
Experiment, a certain amount of above-mentioned mother liquor is diluted with 0.1% Tween 80, is configured to the concentration needed.Carried out with chitosan oligosaccharide composition
Cucumber downy mildew pot experiment, plant investigate downy mildew incidence before the 1st medicine, and set corresponding list substantially in 4 leaf phases
Agent and blank control.Using manual sprayer routine conventional spray, 10 plants are sprayed per medicament, is sprayed again every 7-10d after the 1st spraying
Mist 1 time.10-14d is investigated after 2nd medicine, every plant of whole blades of investigation, record investigation total number of sheets, the sick number of sheets, according to following classification
Method calculates disease index and preventive effect.
Blade staging：
0 grade：It is disease-free
1 grade：The connected area of scab accounts for less than the 1% of whole leaf area；
3 grades：The connected area of scab accounts for the 1%-10% of whole leaf area；
5 grades：The connected area of scab accounts for the 11%-20% of whole leaf area；
7 grades：The connected area of scab accounts for the 21%-50% of whole leaf area；
9 grades：The connected area of scab accounts for more than the 51% of whole leaf area；
X is the preventive effect of active component A when concentration is a, and y is the preventive effect of active component B when concentration is b.
Compare theoretical effect and actual effect, evaluate the synergy of composition, make when being more than theoretical preventive effect using actual preventive effect as synergy
With.It the results are shown in Table 1.
Prevention effect of the composition of table 1 to cucumber downy mildew
The test potted plant to cucumber downy mildew of composition containing Acibenzolar and chitosan oligosaccharide finds (being shown in Table 1), is matched in composition
For 1:8-8:When 1, actual diseases prevention of the composition to cucumber downy mildew is above the theoretical preventive effect of correspondent composition.When composition with
When spraying is applied during 20-50mg/L, composition has higher preventive effect.It is clear that the composition pair containing Acibenzolar and chitosan oligosaccharide
Cucumber downy mildew is synergy.
Prevention effect of the composition of embodiment 2 to wheat diseases
Single compound or the dissolving of composition solvent are prepared into 1000mg/L mother liquor.It is pre- according to biological activity determination
Experiment, a certain amount of above-mentioned mother liquor is diluted with 0.1% Tween 80, is configured to the concentration needed.During experiment, jointing is selected respectively
There are the different fields of white powder disease and wheat rust phase.Single dose and the combination of manual sprayer same concentrations is respectively adopted
Each 1 time of thing routine conventional spray, 20 plants are sprayed per chemicals treatment, and set blank control.Sprayed again 1 time every 7-10d after medicine.2nd time
7-10d is investigated after dispenser.Powdery mildew investigates every plant of whole blade, and leaf, which becomes rusty every plant, investigates 3 leaves in top, with scab face on every leaf
Product accounts for the percentage classification of whole leaf area, carries out the classification of blade powdery mildew according to the method for embodiment 1 and following methods carry out leaf
Piece rust is classified, and calculates disease index and preventive effect.
Blade staging：
0 grade：It is disease-free
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 6%-25% of whole leaf area；
5 grades：Lesion area accounts for the 26%-50% of whole leaf area；
7 grades：Lesion area accounts for the 51%-75% of whole leaf area；
9 grades：Lesion area accounts for more than the 76% of whole leaf area；
Composition is shown in Table 2 and table 3 to the prevention effect of wheat powdery mildew and wheat rust.
Effect of the composition of table 2 to wheat powdery mildew
Effect of the composition of table 3 to wheat rust
From table 2, the composition containing Acibenzolar and chitosan oligosaccharide is 1 in proportioning:5、1:1、5:When 1, composition and phase
When to answer single dose concentration be 30mg/L, composition is above the preventive effect of the single dose under same concentration to the preventive effect of wheat powdery mildew, says
Bright composition is synergy.
From table 3, the composition containing Acibenzolar and chitosan oligosaccharide is 1 in proportioning:3、1:1、3:When 1, composition and phase
When to answer single dose concentration be 28mg/L, composition is above the preventive effect of the single dose under same concentration, explanation to the preventive effect of wheat rust
Composition is synergy.
Preventive effect and effect of increasing production of the composition of embodiment 3 to rape disease
Base fertilizer and division of cells are experimental field used sufficient, is listed, even uniform.Per cell 10m2, per cell with same amount of suitable
Amount running water waters rape seedbed, in order to avoid seedling taking hinders root, Transplanting Density is advisable with every 100 plants of cell.Respectively in tri-leaf period, Hua Qian
Phase, specific aim spraying and each 1 time of root irrigation are carried out with the in a few days medicament corresponding to withing, often cell root spraying volume and concentration are homogeneous
Together, 7-10d investigates the preventive effect of sclerotinia sclerotiorum after medicine.7~10d after 2nd dispenser, often processing are investigated by 10 points, 5 plants every,
Sclerotinia sclerotiorum is classified and statistics is carried out as follows.Received when 2/3 silique is in loquat color in silique in the middle part of yellow green, main shaft
Obtain, the rapeseed weight for different districts of weighing.Period, other control measures all sames of each cell, such as moisture, weeding.Group
Compound the results are shown in Table 4 to the preventive effect and effect of increasing production of sclerotinia sclerotiorum.
Blade staging：
0 grade：It is disease-free；
1 grade：Slight morbidity, onset area account for less than the 5% of stem area；
3 grades：Slight morbidity, onset area account for the 6%-15% of stem area；
5 grades：Medium morbidity, onset area account for the 16%-30% of stem area；
7 grades：Highly fall ill, onset area accounts for the 31%-50% of stem area；
9 grades：Serious morbidity, onset area account for more than the 51% of stem area；
The composition of table 4 is to sclerotinia sclerotiorum and the effect of yield
As known from Table 4, when spray amount is 40mg/L and pouring root amount 10mg/L, at the composition containing Acibenzolar and chitosan oligosaccharide
Manage to the effect and yield of sclerotinia sclerotiorum obviously higher than the effect of single dose, illustrate have containing the composition of Acibenzolar and chitosan oligosaccharide
There are obvious disease-resistant synergy and effect of increasing production.
Find that the composition containing Acibenzolar and chitosan oligosaccharide is in 5-100mg/L, to Portugal by similar method spray test
Grape, apple, strawberry, watermelon, sponge gourd, balsam pear, the preventive effect of the powdery mildew of wax gourd and downy mildew are above the effect of single dose.
Claims (8)
1. the composition containing Acibenzolar and chitosan oligosaccharide, it is characterised in that the weight of Acibenzolar and chitosan oligosaccharide ratio is 1:0.25-4.
2. the purposes of the composition of claim 1, it is characterised in that composition is used for controlling disease, promotes volume increase.
3. purposes according to claim 2, it is characterised in that the disease is the fungus-caused downy mildew of Mastigomycotina, late blight
Disease, peronospora tabacina, the fungus-caused powdery mildew of Ascomycotina, sclerotiniose, head blight, leaf spot, caused by Basidiomycotina
Rust, smut, the fungus-caused bakanae disease of Fungi Imperfecti, scab, wheat rosette stunt, Maize Dwarf caused by virosis
Disease, black streaked dwarf virus of rice.
4. purposes according to claim 3, it is characterised in that the disease is downy mildew, powdery mildew, rust, sclerotiniose.
5. the application process of the composition of claim 1, it is characterised in that the application dose of composition is 5-100mg/L.
6. method according to claim 5, it is characterised in that the application dose of composition is 10-50mg/L.
7. method according to claim 5, it is characterised in that by composition to plant using at least one of spraying and pouring root place
Reason, the composition identical or different with application concentration first is applied again 0-3 times, the application dose of composition is every 7-20d
5-100mg/L。
8. method according to claim 7, it is characterised in that the plant be cucumber, wheat, rape, grape, apple, strawberry,
Watermelon, sponge gourd, balsam pear, wax gourd.
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| CN106962402B (en) * | 2017-04-19 | 2019-05-07 | 江苏徐淮地区淮阴农业科学研究所 | The inducer of inducing wheat anti gibberellic disease |
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