CN105191991A - Biological seed and root soaking preparation and preparation method thereof - Google Patents
Biological seed and root soaking preparation and preparation method thereof Download PDFInfo
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- CN105191991A CN105191991A CN201410292460.6A CN201410292460A CN105191991A CN 105191991 A CN105191991 A CN 105191991A CN 201410292460 A CN201410292460 A CN 201410292460A CN 105191991 A CN105191991 A CN 105191991A
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Abstract
The invention discloses a biological seed and root soaking preparation and a preparation method thereof. The biological seed and root soaking preparation is prepared from, by weight, 10-30 parts of chitosan, 80-120 parts of protein peptides and 8-12 parts of fungal spore powder. The preparation method comprises the steps that, the chitosan with the deacetylation degree above 95% is taken and added into an acetic acid solution with the concentration of 2-5%, the mixed solution is evenly stirred for 3-5 hours to enable the chitosan to be fully dissolved into the acetic acid solution, and a solution A is prepared; 100-800 daltons of the protein peptides are taken and dissolved with water, stirring is performed for 20-40 minutes, then the fungal spore powder (SPF) with the viable count larger than or equal to 10 billons/g and a suspension dispersing agent polyving akohol are added, the mixed solution is continuously stirred for 20-40 minutes, and a solution B is prepared. The biological seed and root soaking preparation has the extremely obvious effects on promoting plant root system growth, speeding up mineralization of macromolecular organic materials in soil around a plant root system and degradation of fixed-state phosphate, increasing the absorption speed of crops to nutrient ingredients and improving the stress resistance of the crops in the breeding process.
Description
Technical field
The present invention relates to a kind of biologic product, particularly relate to a kind of biological seed soaking leaching root preparation and preparation method thereof.
Background technology
Crop is before sowing or transplanting, in order to improve seed germination speed, promote to transplant plant root prosperity, people adopt seed soaking usually, roots soaking agent processes seed or plant root, to improve the seedling quality of crop, strengthen crop anti-adversity in growing process, finally reach the cultivation object of high-quality, high yield.
At present, the seed soaking of plant that China market circulates, leaching root series products, major part, with organic solvent, if amino acid, humic acid etc. be carrier, by compound chemical preparation and bactericide, reaches and promotes seed germination, prevents and treats and upload seedling stage or the object of seed-borne disease.Although this series products has obvious control efficiency to plant disease, to secure contexts such as food, environment, people and animals, it is many unfavorable but to exist.Especially in the spring of low temperature arid, use this type product to soak seed, soak root, very easily seed plumule, plant young root are damaged, occur " burning seed ", " burning root " phenomenon.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of biologic product, a kind of biological seed soaking leaching root preparation and preparation method thereof is especially provided, solve " burning seed ", " burning root " phenomenon that existing similar preparation exists, avoid the injury that seed plumule, plant young root are caused, to promoting plant root growth, the mineralising accelerating the larger molecular organics material in root system of plant surrounding soil, the phosphatic degraded of the state that is fixed, strengthening the absorption rate of crop to nutrient component, the anti-adversity of raising crop growth process that there is fairly obvious effect.
Technical problem to be solved by this invention is achieved through the following technical solutions:
A kind of biological seed soaking leaching root preparation is by the preparation comprising following parts by weight of component raw material components and make: shitosan 10-30, protein peptides 80-120, fungal spore pulvis 8-12; Be preferably: shitosan 20, protein peptides 100, fungal spore pulvis 10.
Described shitosan is the shitosan of deacetylation more than 95%, and described protein peptides is the daltonian protein peptides of 100-800, and described fungal spore pulvis is viable count (cfu) >=10,000,000,000/g fungal spore pulvis (SPF).
Described preparation also comprises the preparation that following parts by weight of component raw material is made further: acetic acid 400-600, water 300-500; Dispersant 4-6, appropriate sodium hydroxide solution pH value regulator.Preferred described parts by weight of raw materials component is: acetic acid 465, water 400; Dispersant 5.
Preferred described acetic acid solution is the acetic acid solution of 2-5%; Described water is room temperature water; Described dispersant is polyvinyl alcohol or hydroxypropyl methylcellulose suspension dispersive agent; Described sodium hydroxide solution is 0.1mol/L sodium hydroxide solution.
Present invention also offers a kind of preparation method of described biology seed soaking leaching root preparation, comprise the following steps:
Get shitosan, add in acetic acid solution, uniform stirring, shitosan is fully dissolved among acetic acid solution and makes solution A;
Get protein peptides, use water-soluble solution, stir and add fungal spore pulvis (SPF), suspension dispersive agent, continue stirring and make B solution;
Described solution A is all spare in described B solution, stir, with sodium hydroxide solution, the pH value of mixing material is adjusted to 4-5 and makes biological seed soaking roots soaking agent.
Preferred:
(1) get the shitosan of deacetylation more than 95%, at ambient temperature, joining concentration is in the acetic acid solution of 2-5%, uniform stirring 3-5 hour, shitosan is fully dissolved among acetic acid solution and makes solution A;
(2) the daltonian protein peptides of 100-800 is got, dissolve with room temperature water, 20-40 minute, viable count (cfu) >=10,000,000,000/g fungal spore pulvis (SPF), suspension dispersive agent polyvinyl alcohol (PVA) is added after stirring 30 minutes, continue to stir 20-40 minute, after 30 minutes, make B solution;
(3) described solution A is all spare in described B solution, stir 3-5 hour, with 0.1mol/L sodium hydroxide solution, the pH value of mixing material is adjusted to 4-5 and makes biological seed soaking roots soaking agent.
In the present invention's biology seed soaking leaching root preparation, main additive mass shell glycan, is a kind of natural plant nutrition promoter, can plays desinsection, disease-resistant effect, can improve again the immunocompetence of plant, for plant provides rich in protein nutrition.
The present invention's another kind of interpolation material protein peptides is a kind of molecular weight is the daltonian little peptide material of 100-800, and they are made up of 2-10 amino acid usually.Little peptide is protein fragments accurately, and plant very easily absorbs.Little peptide material, to raising physiologically active of plant, improves plant photosynthesis efficiency, improves the conversion of carbohydrate, has fairly obvious effect.
The preferred described fungal spore pulvis (SporesPowderFungus) of the present invention, by Canadian favor bio tech ltd (GracelandBiotechINC.) by making the solid fermentation of " Penicilliu-Viridicatum " fungi.
The present invention's biology seed soaking leaching root preparation adopts technique scheme compared with prior art, has following beneficial effect:
1. the present invention is biology seed soaking (leaching root) agent that raw material is produced with shitosan, fungal spore pulvis (SPF), protein peptides, and it is not containing any harmful element, fool proof to plant, environment, people and animals.
2. the metabolite that produced in soil by SPF of invention formulation, effectively can be suppressed the soil of crop to pass and seed-borne disease, reduce the probability of happening of disease in plant growing process.After SPF enters soil, a large amount of physiological activators can also be secreted, they are to promoting plant root growth, the mineralising accelerating the larger molecular organics material in root system of plant surrounding soil, the phosphatic degraded of the state that is fixed, strengthening the absorption rate of crop to nutrient component, the anti-adversity of raising crop growth process to have fairly obvious effect.
3. the dormant state fungal spore in invention formulation, after entering soil by seed soaking leaching root mode, just start within one week to sprout, sprout the various metabolites that spore produces in rhizosphere soil, to flourishing root system of plant, improve nutrient absorption rate, prevent and treat all kinds of soil pass, seed-borne disease, improve the anti-adversity of crop, there is very significant effect.
Accompanying drawing explanation
Fig. 1 is that the present invention's biology seed soaking roots soaking agent affects comparing result figure with roots soaking agent of soaking seed on routine to root system development
Fig. 2 is that the present invention's biology seed soaking roots soaking agent affects comparing result figure with roots soaking agent of soaking seed on routine to root development
Fig. 3 be the present invention's biology seed soaking roots soaking agent with routine is soaked seed to roots soaking agent gives birth to Maize at Seedling Stage affect comparing result figure
Embodiment
Below in conjunction with embodiment, the present invention is described further:
Embodiment 1
A kind of biological seed soaking roots soaking agent, raw material components is (kg): the shitosan 20 of deacetylation 95%, 100-800 dalton protein peptide 100, the fungal spore pulvis 10 of viable count (cfu) >=10,000,000,000/g, concentration is the acetic acid solution 465 of 2%, room temperature water 400, suspension dispersive agent polyvinyl alcohol (PVA) 5,0.1mol/L sodium hydroxide solution.
Preparation method: the shitosan getting above-mentioned deacetylation 95%, at ambient temperature, joining concentration is in the acetic acid solution of 2%, and uniform stirring 3 hours makes shitosan fully be dissolved among acetic acid solution and makes solution A; Get described protein peptides, dissolve with room temperature water, stir after 30 minutes and add viable count (cfu) >=10,000,000,000/g fungal spore pulvis, polyvinyl alcohol 5kg, continue stirring and make B solution in 30 minutes; Solution A is all spare in B solution, stirs 3 hours, with 0.1mol/L sodium hydroxide solution, the pH value of mixing material is adjusted to 4 and makes biological seed soaking roots soaking agent.
Embodiment 2
A kind of biological seed soaking roots soaking agent, raw material components is (kg): the shitosan 10 of deacetylation 95%, 100-800 dalton protein peptide 80, the fungal spore pulvis 8 of viable count (cfu) >=10,000,000,000/g, the acetic acid solution 400 of 5%, room temperature water 300, polyvinyl alcohol (PVA) 4kg, appropriate sodium hydroxide solution.
Preparation method: the shitosan getting above-mentioned deacetylation 99%, at ambient temperature, joining concentration is in the acetic acid solution of 5%, and uniform stirring 4 hours makes shitosan fully be dissolved among acetic acid solution and makes solution A; Get described protein peptides, dissolve with room temperature water, stir after 20 minutes and add viable count (cfu) >=10,000,000,000/g fungal spore pulvis, polyvinyl alcohol, continue stirring and make B solution in 20 minutes; Solution A is all spare in B solution, stirs 4 hours, with sodium hydroxide solution, the pH value of mixing material is adjusted to 5 and makes biological seed soaking roots soaking agent.
Embodiment 3
A kind of biological seed soaking roots soaking agent, raw material components is (kg): the shitosan 30 of deacetylation 95%, 100-800 dalton protein peptide 120, the fungal spore pulvis 12 of viable count (cfu) >=10,000,000,000/g, the acetic acid solution 600 of 4%, water 500, polyvinyl alcohol (PVA) 6kg, appropriate 0.2 or 0.3mol/L sodium hydroxide solution.
Preparation method: the shitosan getting above-mentioned deacetylation 96%, at ambient temperature, joining concentration is in the acetic acid solution of 4%, and uniform stirring 5 hours makes shitosan fully be dissolved among acetic acid solution and makes solution A; Get described protein peptides, dissolve with room temperature water, stir after 40 minutes and add viable count (cfu) >=10,000,000,000/g fungal spore pulvis, polyvinyl alcohol, continue stirring and make B solution in 40 minutes; Solution A is all spare in B solution, stirs 5 hours, with sodium hydroxide solution, the pH value of mixing material is adjusted to 4.5 and makes biological seed soaking roots soaking agent.
Embodiment 1 biology seed soaking roots soaking agent affects comparing result figure with roots soaking agent of soaking seed on routine to root system development as shown in Figure 1, and invention formulation has the effect of better growth promoting effects and growth to rice root.
Fig. 2 is that embodiment 1 biology seed soaking roots soaking agent affects comparing result figure with roots soaking agent of soaking seed on routine to root development, and invention formulation has the effect of better growth promoting effects and growth to maize root system.
Fig. 3 be embodiment 1 biology seed soaking roots soaking agent with routine is soaked seed to roots soaking agent gives birth to Maize at Seedling Stage affect comparing result, invention formulation has the effect of better growth promoting effects and growth to Maize at Seedling Stage.
The experiment and demonstration of embodiment 1:
Embodiment 1 experiment and demonstration result shows, soak seed with routine, compared with roots soaking agent, the seed soaking of the present invention's biology, roots soaking agent is not weighed has obvious facilitation to raising Plant Height of Rice, and has very significant effect to the ground of plant and underground part fresh weight, dry weight yet.As shown in table 1, table 2:
Table 1 Rice in Hei Longjiang Province seed soaking trial result (50 strains are average)
Harbin City, Heilongjiang Province as shown in table 2 rice seed soaking result of the test (50 strain gross weight)
Claims (8)
1. a biological seed soaking leaching root preparation is by the preparation comprising following parts by weight of component raw material components and make: shitosan 10-30, protein peptides 80-120, fungal spore pulvis 8-12.
2. biology seed soaking leaching root preparation according to claim 1, described parts by weight of raw materials component is: shitosan 20, protein peptides 100, fungal spore pulvis 10.
3. biology seed soaking leaching root preparation according to claim 1, in described raw material components: described shitosan is the shitosan of deacetylation more than 95%, described protein peptides is the daltonian protein peptides of 100-800, and described fungal spore pulvis is the fungal spore pulvis of viable count (cfu) >=10,000,000,000/g.
4. the biology seed soaking leaching root preparation according to claim 1,2 or 3, described preparation also comprises the preparation that following parts by weight of component raw material is made further: acetic acid 400-600, water 300-500; Dispersant 4-6, appropriate sodium hydroxide solution pH value regulator.
5. biology seed soaking leaching root preparation according to claim 4, described parts by weight of raw materials component is: acetic acid 465, water 400; Dispersant 5.
6. biology seed soaking leaching root preparation according to claim 4, described acetic acid solution is the acetic acid solution of 2-5%; Described water is room temperature water; Described dispersant is polyvinyl alcohol or hydroxypropyl methylcellulose suspension dispersive agent: described sodium hydroxide solution is 0.1mol/L sodium hydroxide solution.
7. the preparation method of one of claim 1-6 described biology seed soaking leaching root preparation, comprises the following steps:
Get shitosan, add in acetic acid solution, uniform stirring, shitosan is fully dissolved among acetic acid solution and makes solution A;
Get protein peptides, use water-soluble solution, stir and add fungal spore pulvis, suspension dispersive agent, continue stirring and make B solution;
Described solution A is all spare in described B solution, stir, with sodium hydroxide solution, the pH value of mixing material is adjusted to 4-5, obtained biological seed soaking roots soaking agent.
8. the preparation method of root preparation is soaked in a kind of described biology seed soaking according to claim 7, and described step is further:
Get the shitosan of deacetylation more than 95%, at ambient temperature, joining concentration is in the acetic acid solution of 2-5%, uniform stirring 3-5 hour, shitosan is fully dissolved among acetic acid solution and makes solution A;
Get the daltonian protein peptides of 100-800, dissolve with room temperature water, 20-40 minute, viable count (cfu) >=10,000,000,000/g fungal spore pulvis, suspension dispersive agent polyvinyl alcohol (PVA) is added after stirring 30 minutes, continue to stir 20-40 minute, after 30 minutes, make B solution;
Described solution A is all spare in described B solution, stir 3-5 hour, with 0.1mol/L sodium hydroxide solution, the pH value of mixing material is adjusted to 4-5 and makes biological seed soaking roots soaking agent.
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| CN201410292460.6A CN105191991A (en) | 2014-06-27 | 2014-06-27 | Biological seed and root soaking preparation and preparation method thereof |
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| CN201410292460.6A CN105191991A (en) | 2014-06-27 | 2014-06-27 | Biological seed and root soaking preparation and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109717038A (en) * | 2019-03-13 | 2019-05-07 | 四川茂承生物科技有限公司 | Radix Salviae Miltiorrhizae high quality seedling breeding method |
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| CN101049109A (en) * | 2006-04-05 | 2007-10-10 | 东北农业大学 | Nuisance free coating agent for seeds and preparation method |
| CN101120673A (en) * | 2007-09-19 | 2008-02-13 | 雷庆峰 | Nontoxic pesticide and preparing method thereof |
| CN102302037A (en) * | 2010-05-09 | 2012-01-04 | 海南正业中农高科股份有限公司 | Bactericide pesticide composition containing chitosan oligosaccharide |
| WO2013040366A1 (en) * | 2011-09-14 | 2013-03-21 | Novozymes Biologicals Holdings A/S | Use of lipochito-oligosaccharides and/or chito oligosaccharides in combination with phosphate-solubilizing microorganisms to enhance plant growth |
| CN103554236A (en) * | 2013-11-05 | 2014-02-05 | 李�昊 | Short peptide for promoting plants to take roots, sprout, grow and prolong storage period and application thereof |
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2014
- 2014-06-27 CN CN201410292460.6A patent/CN105191991A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101049109A (en) * | 2006-04-05 | 2007-10-10 | 东北农业大学 | Nuisance free coating agent for seeds and preparation method |
| CN101120673A (en) * | 2007-09-19 | 2008-02-13 | 雷庆峰 | Nontoxic pesticide and preparing method thereof |
| CN102302037A (en) * | 2010-05-09 | 2012-01-04 | 海南正业中农高科股份有限公司 | Bactericide pesticide composition containing chitosan oligosaccharide |
| WO2013040366A1 (en) * | 2011-09-14 | 2013-03-21 | Novozymes Biologicals Holdings A/S | Use of lipochito-oligosaccharides and/or chito oligosaccharides in combination with phosphate-solubilizing microorganisms to enhance plant growth |
| CN103554236A (en) * | 2013-11-05 | 2014-02-05 | 李�昊 | Short peptide for promoting plants to take roots, sprout, grow and prolong storage period and application thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109717038A (en) * | 2019-03-13 | 2019-05-07 | 四川茂承生物科技有限公司 | Radix Salviae Miltiorrhizae high quality seedling breeding method |
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