CN103554236A - Short peptide for promoting plants to take roots, sprout, grow and prolong storage period and application thereof - Google Patents
Short peptide for promoting plants to take roots, sprout, grow and prolong storage period and application thereof Download PDFInfo
- Publication number
- CN103554236A CN103554236A CN201310538399.4A CN201310538399A CN103554236A CN 103554236 A CN103554236 A CN 103554236A CN 201310538399 A CN201310538399 A CN 201310538399A CN 103554236 A CN103554236 A CN 103554236A
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- small peptide
- tyr
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- germination
- val
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Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
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Abstract
The invention provides a short peptide for promoting plants to take roots, sprout, grow and prolong storage period and an application thereof. The main sequences of amino acids of short peptide fragments, analogues, derivatives and variants of the short peptide have not less than 70% of similarity and not less than 90% of similarity of the main sequence of short peptide amino acid; the invention also discloses functional products for promoting plant seeds to take roots, sprout, grow and prolong the storage period prepared by using the short peptide, nucleotide, short peptide fragments, short peptide analogues, short peptide derivatives and short peptide variants, and an application of the functional products. The molecular structure of the short peptide related by the invention is clear, the production technique is mature, finished products are non-toxic, non-harm and free of side effects and have obvious characteristics of promoting plant seeds to take roots, sprout, grow and prolong the storage period.
Description
Technical field
The invention belongs to biotechnology agriculture field, the present invention has more specifically related to a kind of small peptide and application thereof that promotes plant establishment, germination, growth and prolongation storage period.
Background technology
Taking root, germinate, grow and storing of plant is the problem of agricultural development most critical always.The material that can promote plant seed Rapid Rooting, germination, growth is being badly in need of of Current Agricultural Development.And plant hormone is in the market mostly plant hormone, in growth hormone, most important chemical substance is 3-indolyl acetic acid.The similar plant-growth regulator of synthetic effect also has rapin, phytokinin, Plant hormones regulators,gibberellins, Nafusaku, amine DA-6 (DA-6) etc.Such growth hormone has duality, can promote growth can suppress again growth.Be that lower concentration promotes growth, high density suppresses growth.These plant hormones mostly have acute oral toxicity and acute dermal toxicity, and therefore, the plant hormone of seeking a kind of nontoxic non-hormone having no side effect is that agricultural development people's expectations is required.And with biologically active peptides---coming from the formed small peptide of aminoacid sequence of the fragment of protein, as the product application of taking root, germinate, growing and storing of plant, is a kind of new demand.
For the long-term storage of seed or fruit and storage or the long-distance transport of short-term, also extremely important aspect industrial or agricultural.Mainly to prevent that seed or fruit from being occurred putrid and deteriorated by bacterium intrusion.Conventional sanitas and preservation agent are all compounds, and as phenylformic acid, butylated hydroxytoluene etc., these compounds are mostly virose; Also have natural preservation agent as Nutgalls propionic ester, vitamin-E, spice extract etc., and New Cut Flower Fresh Keeping is as lacticin, N,O-Diacetylmuramidase etc.; The multiplex Trisodium Citrate of food preservative, Sodium Pyrosulfite etc.These sanitass or preservation agent have its optimal working substance, in its specific scope, play stronger prolongation storage period or the effect of shelf lives.Wherein, lacticin (Nisin) is a polypeptide being comprised of 34 amino acid, the high food of protein content had and pretends use, and by lower than 10 small peptides below amino acid, be used in aspect storage period that extends seed or fruit, yet there are no bibliographical information.
Therefore, development can either promote plant establishment, germination, growth, and the bioactive short peptide that can extend again storage period and application tool thereof are of great significance and are worth.
Summary of the invention
The present invention seeks to for the problems referred to above, a kind of small peptide and application thereof that promotes plant establishment, germination, growth and prolongation storage period be provided, it is characterized in that:
The aminoacid sequence of <1> composition small peptide is as follows:
Tyr-Leu-Tyr-Glu-Val-Gly-Arg or
Tyr-Leu-Tyr-Glu-Val-Ala-Arg or
Tyr-Leu-Tyr-Glu-Val-Ile-Arg or
Tyr-Leu-Tyr-Glu-Val-Val-Arg or
Tyr-Leu-Tyr-Glu-Val-Leu-Arg or
Tyr-Ile-Tyr-Glu-Val-Ala-Arg or
Tyr-Ile-Tyr-Glu-Val-Ala-Lys or
Tyr-Ile-Tyr-Glu-Gly-Gly-Lys
Described in <2>, the nucleotide sequence of small peptide refers to the gene order that corresponding 3 codons of each aminoacid sequence of small peptide form.
A kind of small peptide fragment that promotes the small peptide of plant establishment, germination, growth and prolongation storage period of the present invention, it is characterized in that: 3 or 4 or 5 or 6 amino acid form described small peptide fragment in order 7 described amino acid whose sequences are intercepted into from optional position, and there is the small peptide fragment that promotes plant germination, growth and prolongation storage period.
A kind of small peptide fragment that promotes the small peptide of plant establishment, germination, growth and prolongation storage period of the present invention, it is characterized in that: 3 or 4 or 5 or 6 base sequences corresponding to amino acid form the nucleotide fragments of described small peptide fragment in order the nucleotide sequence of 7 described amino acid whose correspondences is intercepted into from optional position, and there is the small peptide fragment of promotion plant establishment, germination, growth and prolongation storage period.
A kind of analogue that promotes the small peptide of plant establishment, germination, growth and prolongation storage period of the present invention, it is characterized in that: described small peptide analogue refers to and with described small peptide and another kind of compound, merging or merging other polypeptide or protein and form with the aminoacid sequence of described small peptide, and there is peptide sequence or the protein of promotion plant germination, growth and prolongation storage period.
A kind of derivative that promotes the small peptide of plant establishment, germination, growth and prolongation storage period of the present invention, it is characterized in that: after described small peptide derivative refers to amino acid whose one or several amino acid whose certain groups in the amino acid whose sequence of described small peptide are replaced with other group, and there is the small peptide that promotes plant germination, growth and prolongation storage period.
A kind of variant that promotes the small peptide of plant establishment, germination, growth and prolongation storage period of the present invention, it is characterized in that: described small peptide variant refers to a kind of nucleotide sequence that has the aminoacid sequence of one or several amino acid or Nucleotide change or encode it, described change is included in aminoacid sequence or nucleotide sequence, arbitrary topagnosis in the middle of sequence, insertion or replacement amino acid or Nucleotide, or at sequence two ends, add amino acid or Nucleotide, there are sequence homology or similarity with small peptide of the present invention.
A kind of application that promotes the small peptide of plant establishment, germination, growth and prolongation storage period of the present invention, it is characterized in that: described small peptide, Nucleotide, small peptide fragment, small peptide analogue, small peptide derivative and the preparation of small peptide variant have the biological functionality goods that promote that spermatophyte takes root, germinates, grows and extends storage period, and in low strength range, 0.01 mg/L～1 mg/L promotes plant establishment, germinating growth; At high concentration range 1 mg/L～1000 mg/L, suppressing growth is suitable for extending storage period.
A kind of application that promotes the small peptide of plant establishment, germination, growth and prolongation storage period of the present invention, wherein said biological functionality refers to and promotes the phanerogamous application function of taking root, germinate, promote the growth of plant and the period of storage of prolongation plant seed or mature fruit etc.This spermatophyte comprises angiosperm and gymnosperm, including but not limited to greengrocery: leaf vegetables is as Chinese cabbage, and tubers is as potato, radish etc., and fruit vegetables is as cucumber, dish green pepper, fresh kidney beans, beet etc.; Fruits: as sugarcane, melon as watermelon, berry fruit is as strawberry, blueberry, blackberry, blueberry, grape etc., and walnut class is as cherry, peach, date, longan, plum etc., and pomaceous fruit is as apple, pears, loquat, Kiwifruit etc.; Grain class is as paddy, corn, wheat, soybean, Chinese sorghum etc.; Flowers class is as Rose, carnation and Cosmos bipinnatus etc.
Remarkable advantage of the present invention is: small peptide of the present invention comes from and uses the method for information biology that the albumen of international Protein Data Bank is carried out to sequence alignment, a kind of novel biologically active peptides with active function screening, uses small peptide of the present invention taking root, germinate, growing and extending storage period and have remarkable remarkable efficacy plant seed.
accompanying drawing explanation
fig. 1 is that the small peptide the present invention relates to promotes division, the proliferation function related experiment interpretation of result figure of cell in different concns interval.
fig. 2 is that the small peptide the present invention relates to promotes that sugarcane takes root, germination effect lab diagram.
With the small peptide that the present invention relates to, cultivate sugarcane, investigate the taking root of experimental group and control group sugarcane, germination.(1) experimental group sugarcane is to cultivate 7 days with containing small peptide nutrient solution of the present invention, obviously has radical bud longer; (2) control group is not contain small peptide nutrient solution of the present invention, and clearly, radical bud is very short, mutually more far short of what is expected than experimental group.
fig. 3 is that the small peptide fragment of the small peptide intercepting that the present invention relates to promotes the effect lab diagram that sugarcane takes root, germinates and grows.
With the small peptide that the present invention relates to, cultivate sugarcane, investigate the taking root of experimental group and control group sugarcane, germination.(1) experimental group sugarcane is to cultivate 7 days with containing small peptide nutrient solution of the present invention, obviously has radical bud longer; (2) control group is not contain small peptide nutrient solution of the present invention, and clearly, radical bud is very short, mutually more far short of what is expected than experimental group.
fig. 4 is that the small peptide the present invention relates to extends the sugarcane effect lab diagram of storage period.
With the small peptide the present invention relates to, soak sugarcane cross section, then sterile vacuum packing, investigates result for 7 days.(1) changes of section of experimental group sugarcane less than, color presents yellow, with tangent plane color and luster was consistent at that time; (2) there is no the sugarcane of processing through the small peptide that the present invention relates to, its surface presents sorrel, and this is that the polyphenoloxidase of sugarcane causes sugarcane browning reaction.
fig. 5 is that the small peptide variant the present invention relates to extends the sugarcane effect lab diagram of storage period.
With the small peptide the present invention relates to, soak sugarcane cross section, then sterile vacuum packing, investigates result for 7 days.(1) cross section of experimental group sugarcane has no change, and color presents yellow, and tangent plane color and luster is consistent when just cutting; (2) there is no the sugarcane of processing through the small peptide that the present invention relates to, its surface presents sorrel, and this is that the polyphenoloxidase of sugarcane causes sugarcane browning reaction, affects the storage of sugarcane.
Embodiment
The small peptide the present invention relates to, adopts the method for chemosynthesis to prepare, and very ripe solid-phase peptide synthetic method well known in the art, both can adopt Boc method also can adopt Fmoc method.Specific practice is coupled to one by one inertia solid phase carrier by protected amino acid exactly and gets on, and then utilizes strong acid that peptide chain cracking from carrier is got off, and removes side chain protected simultaneously.
The aminoacid sequence of small peptide, Tyr-Leu-Tyr-Glu-Val-Gly-Arg or Tyr-Leu-Tyr-Glu-Val-Ala-Arg or Tyr-Leu-Tyr-Glu-Val-Ile-Arg or Tyr-Leu-Tyr-Glu-Val-Val-Arg or Tyr-Leu-Tyr-Glu-Val-Leu-Arg or
Tyr-Ile-Tyr-Glu-Val-Ala-Arg or Tyr-Ile-Tyr-Glu-Val-Ala-Lys or
Tyr-Ile-Tyr-Glu-Gly-Gly-Lys is main sequence, what from optional position, intercept into 3 or 4 or 5 or 6 amino acid compositions has same bioactive small peptide fragment with described small peptide, small peptide analogue, small peptide derivative, small peptide variant etc. all has efficacy effect of the present invention.
Nucleotide adopts artificial synthesis preparation; The Nucleotide of coding small peptide comprises a kind of in lower group:
(a) coding has the small peptide of described aminoacid sequence or the Nucleotide of its fragment, analogue, derivative or its variant;
(b) with (a) Nucleotide of described Nucleotide complementation;
(c) with (a) or the Nucleotide of have >=75% homogeny of Nucleotide (b);
The Nucleotide that this Nucleotide has an aminoacid sequence small peptide described in <1> by coding substantially forms, nucleotide sequence of the present invention, and its form is the DNA that DNA form comprises cDNA or synthetic.DNA can be strand can be also double-stranded.The coding region sequence of coding small peptide can be identical with the Nucleotide in <2>, also can be different, be called varient, wherein varient refers to the coding with the aminoacid sequence in coding claim 1, but can be different with the Nucleotide in <2>.Varient can be the nucleotide sequence that one or several Nucleotide replaces, inserts, lacks, but can not change the active function of the small peptide of aminoacid sequence in coding <1>.
" variant " of this small peptide or Nucleotide refers to a kind of nucleotide sequence that has the aminoacid sequence of one or several amino acid or Nucleotide change or encode it.Change can comprise disappearance, insertion, interpolation or the replacement of amino acid in aminoacid sequence or nucleotide sequence or Nucleotide.Variant can have " conservative property " and change, and the amino acid of wherein replacing has the structure similar with original acid or chemical property, also can have " non-conservation " to change, and the amino acid of wherein replacing does not have the structure similar with original acid or chemical property." disappearance " refers to the disappearance of in aminoacid sequence or nucleotide sequence one or several amino acid or Nucleotide; " insertion " refers in the middle optional position of aminoacid sequence or nucleotide sequence and inserts one or several amino acid; N end or C end that " interpolation " refers at aminoacid sequence or nucleotide sequence add one or several amino acid; " replacement " refers to different amino acid or Nucleotide and replaces one or several amino acid or Nucleotide.
This small peptide, Nucleotide, small peptide fragment, small peptide analogue, small peptide derivative and small peptide variant include but not limited to the purposes of following effect for the preparation of the functional article that promotes plant seed to take root, germinate, grow and to extend storage period:
This spermatophyte comprises angiosperm and gymnosperm, including but not limited to
1. greengrocery: leaf vegetables is as Chinese cabbage, and tubers is as potato, radish etc., and fruit vegetables is as cucumber, dish green pepper, fresh kidney beans, beet etc.;
2. fruits: as sugarcane, melon as watermelon, berry fruit is as strawberry, blueberry, blackberry, blueberry, grape etc., and walnut class is as cherry, peach, date, longan, plum etc., and pomaceous fruit is as apple, pears, loquat, Kiwifruit etc.;
3. grain class: as paddy, corn, wheat, soybean, Chinese sorghum etc.
4. flowers class is as Rose, carnation and Cosmos bipinnatus etc.
When the amino acid chief series that calculates small peptide fragment, small peptide derivative and small peptide variant has with the homogeny of small peptide amino acid chief series and similarity, " homogeny percentage " refers to the identical percentage of sequence during two or more amino acid or nucleotide sequence are relatively.According to Cluster method (Higging D.G. & Sharp P.M., Gene 1988,73:234-237) by checking that distance between all pairings is by each group series arrangement cluster, then by each bunch to distribute in pairs or in groups.Two aminoacid sequences calculate by following formula as the homogeny percentage between sequence A and sequence B:
Wherein, in sequence C, the residue number at interval and the residue number at interval in sequence D do not comprise similar amino acid whose number.
Below in conjunction with embodiment, further set forth the present invention.These implementation examples are only not used in and limit the scope of the invention for the present invention is described.The related all synthetic small peptide of following all embodiment of enumerating all has the function the present invention relates to and is not limited to cited function.
(1) small peptide the present invention relates to promotes division, the proliferation function related experiment of cell
The kinases (LRR-RKs) all existing on the small peptide that application the present invention relates to and the plasma membrane of vegetable cell carries out enzyme activity affects Bioexperiment.Kinases (LRR-PKs) is the proteolytic enzyme that affects cell proliferation, differentiation all existing in all vegetable cells.As shown in Figure 1, in low strength range, 0.01 mg/L～1 mg/L promotes plant establishment, germinating growth to experimental result; At high concentration range 1 mg/L～1000 mg/L, suppressing growth is suitable for extending storage period.
Illustrate: the small peptide the present invention relates to is consistent to the action effect of this phytokinase, at this, does not list one by one.
(2) promote the experiment effect that spermatophyte takes root, germinates, grows
embodiment 1fruits: as sugarcane:
Promote the experiment effect that sugarcane germinates (the synthetic small peptide the present invention relates to: Tyr-Leu-Tyr-Glu-Val-Gly-Arg and Tyr-Leu-Tyr-Glu-Val-Ala-Arg and intercept 4 amino acid: Glu-Val-Ala-Arg in sequence, intercept 5 amino acid: Tyr-Glu-Val-Ala-Arg and 6 amino acid of intercepting: Leu-Tyr-Glu-Val-Ala-Arg)
Adopt solid phase Fmoc method chemosynthesis small peptide Tyr-Leu-Tyr-Glu-Val-Gly-Arg of the present invention, Tyr-Leu-Tyr-Glu-Val-Ala-Arg, Glu-Val-Ala-Arg, Tyr-Glu-Val-Ala-Arg and Leu-Tyr-Glu-Val-Ala-Arg, after HPLC purifying, purity surpasses 98.4%, 97.8%, 95.9%, 99.6% and 96.3%, with ultrapure water, dissolve respectively peptide of the present invention, be prepared into respectively 0.01,0.05,0.1, the solution of 0.5,1 mg/ml.Adopt fresh cane to carry out Seed germination.
Select MS minimum medium, add 3% sucrose, adjust pH to 5.8.In sugarcane stem intercepting even thickness, the intercept of length 7 cm left and right, keeps cross section smooth, with 5% clorox, soaks 15 mins sterilizations.Cane stalk section after sterilization is dipped in the beaker that fills corresponding nutrient solution, and liquid level did not have 2 cm left and right, cross section, bottom.Beaker mouth seals with preservative film, prevents nutrient solution evaporation, and on preservative film, stabs several skies, keeps aeration status, in order to avoid cane stalk section anoxic cannot be grown.24 ~ 28 ℃ of culturing room's temperature, illumination every day, but avoid direct sunlight.
The preparation of MS substratum: macroelement (mother liquor I) mg/L 100ml.(2) trace element (mother liquor II) (3) molysite (mother liquor III) etc.Concrete steps: mother liquor I is each 10ml such as 100 ml, mother liquor II, III, and mother liquor is put into beaker together; Take 54g carrageenin; 300g sucrose; Gac 1g, is placed in glue bucket; Add water and be settled to 1 liter, open stirrer, stir evenly mixed solution, be settled to 10 liters, stir evenly; To mother liquor mixture, add white sugar, continue to stir; Then stop stirrer and add again carrageenin, open stirrer and mix; Then stopping stirrer adds gac again, opens stirrer and stirs evenly; With NaOH, adjust pH till the pH of substratum is 5.8; Substratum is divided and installed in selected culture vessel, cover bottleneck; Sterilizing.
Experimental result shows, with the small peptide the present invention relates to (Tyr-Leu-Tyr-Glu-Val-Ala-Arg), cultivate sugarcane, from cultivating 4 days, the result of experimental group and control group is observed, the small peptide the present invention relates to has the effect (shown in accompanying drawing 2) of stronger stratification to sugarcane, wherein (1) experimental group sugarcane is to cultivate with containing small peptide nutrient solution of the present invention, obviously has radical bud longer; (2) control group is not contain small peptide nutrient solution of the present invention, and clearly, radical bud is very short, mutually more far short of what is expected than experimental group.
Experimental result shows, by the fragment (Tyr-Glu-Val-Ala-Arg) of the small peptide intercepting the present invention relates to, cultivate sugarcane, from cultivating 4 days, the result of experimental group and control group is observed, the small peptide the present invention relates to has the effect (shown in accompanying drawing 3) of stronger stratification to sugarcane, wherein (1) experimental group sugarcane is to cultivate with containing small peptide nutrient solution of the present invention, obviously has radical bud longer, but short compared with shown in Fig. 2 (1); (2) control group is not contain small peptide nutrient solution of the present invention, and clearly, radical bud is very short, mutually more far short of what is expected than experimental group.
Experimental result shows, with the small peptide (Tyr-Leu-Tyr-Glu-Val-Gly-Arg) the present invention relates to, cultivates sugarcane, and from cultivating 4 days, the result of experimental group and control group is observed, and the small peptide the present invention relates to has the effect of stronger stratification to sugarcane.Experimental result and the above results are similar.
Experimental technique similarly, small peptide intercepting 4 amino acid (Tyr-Glu-Val-Ala-Arg) that the present invention relates to, intercept 5 amino acid (Tyr-Glu-Val-Ala-Arg) and intercepting 6 amino acid (Tyr-Glu-Val-Ala-Arg), obtained the effect that similar promotion sugarcane germinates, its action effect is along with the increase of small peptide amino acid quantity, and effect strengthens thereupon.
embodiment 2greengrocery: leaf vegetables is as Chinese cabbage, spinach, celery, mater convolvulus etc.
(the synthetic small peptide derivative the present invention relates to, at tyrosine acids base group modification: the Tyr that phosphorates to promote the experiment effect of Chinese cabbage, spinach, celery, mater convolvulus seed germination
p-Leu-Tyr-Glu-Val-Gly-Arg and Tyr
p-Leu-Tyr
p-Glu-Val-Gly-Arg)
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention, at the R of tyrosine Tyr group, with phosphate group, replace, replace one and two groups, after HPLC purifying, purity surpasses 98%, with ultrapure water, dissolves peptide of the present invention, is prepared into respectively the solution of 10 and 100 ug/ml.
Chinese cabbage: get Chinese cabbage seeds and be placed in 22 ℃ of temperature and contain the small peptide (Tyr the present invention relates to
p-Leu-Tyr-Glu-Val-Gly-Arg) in solution, soak seed, pull out after 6 hours, be placed in vernalization at 15 ℃ of temperature, within 1 day, can germinate, percentage of germination has improved 15% left and right than the seed that does not add the warm water soaking of the small peptide the present invention relates to.
Celery: get celery seed and be placed in the space of 20 ℃～25 ℃ of temperature, with the above-mentioned small peptide (Tyr the present invention relates to
p-Leu-Tyr
p-Glu-Val-Gly-Arg) solution soaking, after 8 hours, is pulled out, and with wet gauze, wraps, be placed in the cool place place vernalization of 20 ℃, and do not add small peptide solution soaking seed of the present invention and think ratio, its germinating time is 4～5 days, shifted to an earlier date 2～3 days, percentage of germination has improved 20% left and right.
Spinach: the small peptide solution soaking spinach seed that use the present invention relates in addition 8 hours, be placed in vernalization at 15 ℃ of temperature, within 1～2 day, can germinate, its percentage of germination has not improved 32% left and right than not adding the small peptide solution the present invention relates to.
Mater convolvulus: get mater convolvulus seed and be placed in 50 ℃～55 ℃ warm water that contain the small peptide the present invention relates to and soak seed, after 5 hours, take out and wrap with wet cloth, vernalization at 25 ℃ of temperature, percentage of germination has improved 24% left and right than the seed that does not add the small peptide solution soaking the present invention relates to.
embodiment 3fruit vegetables is as cucumber, dish green pepper, tomato, eggplant, fresh kidney beans, beet etc.;
Promote fruit vegetables as cucumber, dish green pepper, tomato, eggplant, fresh kidney beans, beet seed germination (the synthetic small peptide the present invention relates to: Tyr-Ile-Tyr-Glu-Val-Ala-Arg and small peptide variant thereof)
Adopt solid phase Fmoc method chemosynthesis small peptide Tyr-Ile-Tyr-Glu-Val-Ala-Arg of the present invention, after HPLC purifying, purity surpasses 99%, with ultrapure water, dissolves peptide of the present invention, is prepared into the solution of 100 ug/ml.
Cucumber: getting cucumber seeds, to be placed in temperature be the above-mentioned small peptide solution the present invention relates to 28 ℃～30 ℃ contain, soaks seed 1 hour, takes out, with wet cloth, wrap, germination time is about 8 hours, and existing sprouting is come up, shortened germinating time, percentage of germination reaches more than 98%.
Fresh kidney beans: getting fresh kidney beans seed, to be placed in temperature be the above-mentioned small peptide solution the present invention relates to 25～30 ℃ contain, soak seed 8 hours, take out, with wet cloth, wrap, germination time is about 3 days, and existing sprouting is come up, shortened germinating time, percentage of germination reaches more than 95%.
Beet: getting beet seed, to be placed in temperature be the above-mentioned small peptide solution the present invention relates to 25 ℃ contain, soaks seed 12 hours, takes out, and with wet cloth, wraps, and germination time is about 2 days, and existing sprouting is come up, and has shortened germinating time, and percentage of germination reaches more than 99%.
Adopt solid phase Fmoc method chemosynthesis small peptide variant of the present invention, at N end, increase a Gla amino acid, synthetic short peptide sequence variant sequence Gla-Tyr-Ile-Tyr-Glu-Val-Ala-Arg, after HPLC purifying, purity surpasses 99.1%, with ultrapure water, dissolve peptide of the present invention, be prepared into the solution of 0.1 mg/ml, pack spraying bottle into.
Dish green pepper: getting dish green pepper seed, to be placed in temperature be the above-mentioned small peptide variant solution the present invention relates to 33 ℃～35 ℃ contain, soaks seed 2 hours, takes out, with wet cloth, wrap, germination time is about 2 days, and existing sprouting is come up, shortened germinating time, percentage of germination reaches more than 95%.
Adopt solid phase Fmoc method chemosynthesis small peptide variant of the present invention, in the middle of small peptide, insert a Tyr amino acid, synthetic short peptide sequence variant sequence Tyr-Ile-Tyr-Glu-Tyr-Val-Ala-Arg, after HPLC purifying, purity surpasses 98.6%, with ultrapure water, dissolve peptide of the present invention, be prepared into the solution of 0.1mg/ml, pack spraying bottle into.
Tomato: getting tomato seeds, to be placed in temperature be the above-mentioned small peptide variant solution the present invention relates to 28 ℃～30 ℃ contain, soaks seed 2 hours, takes out, with wet cloth, wrap, germination time is about 1 day, and existing sprouting is come up, shortened germinating time, percentage of germination reaches more than 95%.
Adopt solid phase Fmoc method chemosynthesis small peptide variant of the present invention, in the middle of small peptide, Glu is replaced to a Tyr amino acid, synthetic short peptide sequence variant sequence Tyr-Ile-Tyr-Tyr-Val-Ala-Arg, after HPLC purifying, purity surpasses 97.6%, with ultrapure water, dissolve peptide of the present invention, be prepared into the solution of 0.1 mg/ml, pack spraying bottle into.
Eggplant: getting eggplant seed, to be placed in temperature be the above-mentioned small peptide variant solution the present invention relates to 30 ℃ contain, soaks seed 3 hours, takes out, with wet cloth, wrap, germination time is about 2.5 days, and existing sprouting is come up, shortened germinating time, percentage of germination reaches more than 95%.
Same, small peptide variant Gla-Tyr-Leu-Tyr-Glu-Val-Ile-Arg, Tyr-Leu-Tyr-Glu-Tyr-Val-Ile-Arg and Tyr-Leu-Tyr-Tyr-Val-Ala-Arg have also been done to corresponding short long experiment, the experimental result of small peptide variant Gla-Tyr-Leu-Tyr-Glu-Val-Ala-Arg in experimental result and the present embodiment is without significant difference, therefore do not repeat at this.
embodiment 4tubers is as potato, Ipomoea batatas, radish etc.
Promote effect experiment (the synthetic small peptide the present invention relates to: Tyr-Leu-Tyr-Glu-Val-Val-Arg) that tubers germinates as potato, Ipomoea batatas
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention, after HPLC purifying, purity surpasses 98.5%, with ultrapure water, dissolves peptide of the present invention, is prepared into the solution of 0.1 mg/ml.
Potato: it is core that potato is had to the part of potential bud, be cut into the stem tuber of about 3.5 centimetres sizes, be placed in and contain the small peptide solution the present invention relates to, 16～18 ℃ of temperature, soak 1 hour, stoped the brown stain of potato tangent plane polyphenoloxidase, place in wet environment, within 1～2 day, germinate afterwards, percentage of germination reaches more than 95%.Than not improved more than 15% with the control group percentage of germination that small peptide solution-treated of the present invention is crossed.
embodiment 5melon as watermelon, muskmelon, hami melon
Promote the experiment of the melon effect as watermelon, muskmelon, hami melon seed germination (the synthetic small peptide the present invention relates to:
Tyr-Leu-Tyr-Glu-Val-Ile-Arg）
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention, after HPLC purifying, purity surpasses 99.1%, with ultrapure water, dissolves peptide of the present invention, is prepared into the solution of 0.1 mg/ml.
Watermelon: getting watermelon seed, to be placed in temperature be the small peptide solution the present invention relates to 25 ℃ contain, soaks seed 1 hour, takes out, and with wet cloth, wraps, and germination time is about 1 day, and existing sprouting is come up, and has shortened germinating time, and percentage of germination reaches more than 99%.
Muskmelon: getting muskmelon seed, to be placed in temperature be the small peptide solution the present invention relates to 28～30 ℃ contain, soaks seed 3 hours, takes out, and with wet cloth, wraps, and germination time is about 1 day, and existing sprouting is come up, and has shortened germinating time, and percentage of germination reaches more than 95%.
Hami melon: getting hami melon seed, to be placed in temperature be the small peptide solution the present invention relates to 40 ℃ contain, soaks seed 12 hours, takes out, with wet cloth, wrap, be placed in 30 ℃ of temperature, germination time is about 3 days, existing sprouting is come up, and has shortened germinating time, and percentage of germination reaches more than 95%.
embodiment 6berry fruit is as strawberry, blueberry, blackberry, blueberry, grape etc.
The experiment effect that promotion berry fruit germinates as strawberry, blueberry, blackberry, blueberry, seed of Fructus Vitis viniferae (the synthetic small peptide the present invention relates to:
Tyr-Leu-Tyr-Glu-Val-Leu-Arg）
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention, after HPLC purifying, purity surpasses 97.1%, with ultrapure water, dissolves peptide of the present invention, is prepared into the solution of 0.1 mg/ml.
strawberry: getting strawberry seed, to be placed in temperature be the small peptide solution the present invention relates to 60 ℃ contain, ceaselessly stir, until water temperature is down to 25 ℃ of left and right, take out, be used in the wet cloth soaking in the small peptide solution the present invention relates to and wrap, be placed in 25 ℃～30 ℃ temperature, germination time is about 3 days, existing sprouting shows money or valuables one carries unintentionally and comes up, and has shortened germinating time, and percentage of germination reaches more than 95%.
Blueberry: getting blueberry seed, to be placed in temperature be the small peptide solution the present invention relates to 23 ℃ contain, soak seed 12 hours, take out, be used in the wet cloth soaking in the small peptide solution the present invention relates to and wrap, be placed in 20 ℃～25 ℃ temperature, germination time is about 15 days, there is the sprouting of sending out, and control group (containing small peptide solution soaking of the present invention) comparison, having shortened germinating time two weeks, percentage of germination reaches more than 50%.
Blackberry, blueberry: getting blackberry, blueberry seed, to be placed in temperature be (compound method of MS substratum is identical with the present embodiment 1 sugarcane) on the MS substratum of 25 ℃, in this substratum, add the small peptide the present invention relates to, soak seed 12 hours, take out, be used in the wet cloth soaking in the small peptide solution the present invention relates to and wrap, be placed in 20 ℃～25 ℃ temperature, cultivate dark place, germination time is about 15 days, and its percentage of germination reaches more than 70%.
Grape: getting seed of Fructus Vitis viniferae, to be placed in temperature be the small peptide solution the present invention relates to 40 ℃ contain, soak seed 24 hours, take out, be used in the wet cloth soaking in the small peptide solution the present invention relates to and wrap, be placed in 20 ℃～25 ℃ temperature, germination time is about 15 days, there is the sprouting of sending out, and control group (containing small peptide solution soaking of the present invention) comparison, having shortened germinating time two weeks, percentage of germination reaches more than 63%.
embodiment 7walnut class is as cherry, peach, date, longan etc.
Promote walnut class as cherry, peach, date: experiment effect (the synthetic small peptide the present invention relates to: Tyr-Ile-Tyr-Glu-Val-Ala-Lys) of longan, the growth of plum seed germination
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention, after HPLC purifying, purity surpasses 97.1%, with ultrapure water, dissolves peptide of the present invention, is prepared into the solution of 0.1 mg/ml.
Cherry: getting cherry seed, to be placed in temperature be the small peptide solution the present invention relates to 25 ℃ contain, soak seed 12 hours, take out, be used in the wet cloth soaking in the small peptide solution the present invention relates to and wrap, be placed in 20 ℃～25 ℃ temperature, germination time is about 7 days, have the sprouting of sending out, and control group (not containing small peptide solution soaking of the present invention) relatively, has shortened germinating time, improved percentage of germination, can reach more than 59%.
Peach: get peach seed and be placed in the small peptide solution that containing of 25 ℃ of temperature the present invention relates to, soak seed 24 hours, take out, be used in the wet cloth soaking in the small peptide solution the present invention relates to and wrap, be placed in 20 ℃～25 ℃ temperature, germination time is about 7 days, have the sprouting of sending out, and control group (not containing small peptide solution soaking of the present invention) relatively, has shortened germinating time, improved percentage of germination, can reach more than 95%.
Date: getting date seed, to be placed in temperature be that on the MS substratum of 25 ℃, (compound method of MS substratum is identical with the present embodiment 1 sugarcane, but ph value is transferred to 6.5), in this substratum, add the small peptide the present invention relates to, soak seed 12 hours, take out, be used in the wet cloth soaking in the small peptide solution the present invention relates to and wrap, be placed in 23 ℃～25 ℃ temperature, germination time is about 2 weeks, and its rate of rooting reaches more than 85%.
Longan: get longan seed and be placed in the small peptide solution that containing of 25 ℃ of temperature the present invention relates to, soak seed 2 days, take out, be used in the wet cloth soaking in the small peptide solution the present invention relates to and wrap, be placed in 20 ℃～25 ℃ temperature, germination time is about 3 days, have the sprouting of sending out, and control group (not containing small peptide solution soaking of the present invention) relatively, has shortened germinating time, improved percentage of germination, can reach more than 95%.
embodiment 8pomaceous fruit is as apple, pears, Kiwifruit etc.
Promote walnut class if pomaceous fruit is as experiment effect (the synthetic small peptide the present invention relates to: Tyr-Ile-Tyr-Glu-Gly-Gly-Lys) of apple, pears, loquat, Seed Germination of Kiwifruit growth
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention, after HPLC purifying, purity surpasses 97.9%, with ultrapure water, dissolves peptide of the present invention, is prepared into the solution of 0.1 mg/ml.
Apple: get Apple Seeds and be placed in the small peptide solution that containing of 25 ℃ of temperature the present invention relates to, soak seed 12 hours, take out, strip off kind skin, be used in the wet cloth soaking in the small peptide solution the present invention relates to and wrap, be placed in 22 ℃～25 ℃ temperature, germination time is about 2 days, there is the sprouting of sending out, with control group (not containing small peptide solution soaking of the present invention) is relatively, fast 2～3 days, shortened germinating time, improved percentage of germination, can reach more than 95%.
Pears: get sand pear seeds and be placed in the small peptide solution that containing of 25 ℃ of temperature the present invention relates to, soak seed 24 hours, take out, strip off kind skin, shells, and is used in the wet cloth soaking in the small peptide solution the present invention relates to and wraps, be placed in 22 ℃～25 ℃ temperature, germination time is about 3 days, has the sprouting of sending out, and control group (not containing small peptide solution soaking of the present invention) relatively, fast 2～3 days, shortened germinating time, improved percentage of germination, can reach more than 80%.
embodiment 9grain class is as paddy, corn, wheat, soybean, Chinese sorghum etc.
Promote grain class as the experiment effect of paddy, corn, wheat, soybean, sorghum seeds germinating growth (the synthetic small peptide the present invention relates to: Tyr-Leu-Tyr-Glu-Val-Ala-Lys, increase a Gla amino acid short peptide variant (Gla-Tyr-Ile-Tyr-Glu-Val-Ala-Arg, insert Tyr amino acid short peptide variant a: Tyr-Ile-Tyr-Glu-Tyr-Val-Ala-Arg, Glu replaces to a Tyr small peptide variant Tyr-Ile-Tyr-Tyr-Val-Ala-Arg)
Paddy:
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention (Tyr-Leu-Tyr-Glu-Val-Ala-Lys), after HPLC purifying, purity surpasses 97.1%, with ultrapure water, dissolves peptide of the present invention, is prepared into the solution of 0.1 mg/ml.
Get japonica rice seed and be placed in the small peptide solution that containing of 30 ℃ of temperature the present invention relates to, soak seed 2 hours, take out, be used in the wet cloth soaking in the small peptide solution the present invention relates to and wrap, be placed in 25 ℃ of temperature, germination time is about 1 day, there is the sprouting of sending out, and control group (containing small peptide solution soaking of the present invention) comparison, having improved percentage of germination, can reach more than 95%.
Corn:
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention, increase a Gla amino acid short peptide variant (Gla-Tyr-Ile-Tyr-Glu-Val-Ala-Arg)), after HPLC purifying, purity surpasses 98.2%, with ultrapure water, dissolve peptide of the present invention, be prepared into the solution of 0.1 mg/ml.
Get corn seed and be placed in the small peptide solution that containing of 33 ℃ of temperature the present invention relates to, soak seed 6 hours, take out, be used in the wet cloth soaking in the small peptide solution the present invention relates to and wrap, be placed in 25 ℃ of temperature, germination time is about 3 days, has the sprouting of sending out, and control group (not containing small peptide solution soaking of the present invention) relatively, shortened germinating time, shifted to an earlier date 2 days, and improved percentage of germination, can reach more than 98%.
Wheat:
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention, insert a Tyr amino acid short peptide variant: Tyr-Ile-Tyr-Glu-Tyr-Val-Ala-Arg), after HPLC purifying, purity surpasses 96.4%, with ultrapure water, dissolve peptide of the present invention, be prepared into the solution of 0.1 mg/ml.
Get wheat seed and be placed in the small peptide solution that containing of 30 ℃ of temperature the present invention relates to, soak seed 1 hour, take out, being used in the wet cloth soaking in the small peptide solution the present invention relates to wraps, be placed in 25 ℃ of temperature, germination time is about 2 days, has the sprouting of sending out, and control group (not containing small peptide solution soaking of the present invention) relatively, shorten germinating time, shifted to an earlier date 1 day, and improved percentage of germination, can reach more than 98%, than control group, improve 27%.
Soybean:
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention, Glu is replaced to a Tyr small peptide variant Tyr-Ile-Tyr-Tyr-Val-Ala-Arg, and after HPLC purifying, purity surpasses 97.1%, with ultrapure water, dissolve peptide of the present invention, be prepared into the solution of 0.1 mg/ml.
Get soybean seeds and be placed in the small peptide solution that containing of 20 ℃ of temperature the present invention relates to, soak seed 6 hours, take out, being used in the wet cloth soaking in the small peptide solution the present invention relates to wraps, be placed in 15 ℃ of temperature, germination time is about 3 days, has the sprouting of sending out, and control group (not containing small peptide solution soaking of the present invention) relatively, shorten germinating time, shifted to an earlier date 1 day, and improved percentage of germination, can reach more than 98%, than control group, improve 21%.
Chinese sorghum: get sorghum seeds and be placed in the small peptide solution that containing of 25 ℃ of temperature the present invention relates to, soak seed 6 hours, take out, being used in the wet cloth soaking in the small peptide solution the present invention relates to wraps, be placed in 25 ℃～30 ℃ Fluctuation temperature cultures, germination time is about 2 days, has the sprouting of sending out, and control group (not containing small peptide solution soaking of the present invention) relatively, shorten germinating time, shifted to an earlier date 1 day, and improved percentage of germination, can reach more than 97%, than control group, improve 25%.
embodiment 10flowers class is as rose, carnation and Cosmos bipinnatus etc.
Promote flowers class as experiment effect (the synthetic small peptide the present invention relates to: Tyr-Leu-Tyr-Glu-Val-Ala-Lys) of rose, carnation and the growth of Cosmos bipinnatus seed germination
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention, after HPLC purifying, purity surpasses 96.3%, with physiological saline PBS, dissolves peptide of the present invention, is prepared into the solution of 0.1 mg/ml.
Get respectively the seed of Rose, carnation and Persian chrysanthemum, be placed in respectively the above-mentioned solution of preparing with the small peptide the present invention relates to, soak seed after 2 hours, pull out, on the bundle of crossing by this invention small peptide solution soaking, be placed at the temperature of 20 ℃～25 ℃, after 1 day, both had the germination that shows money or valuables one carries unintentionally.The above-mentioned flowers percentage of germination of the method reaches more than 90%, has improved widely percentage of germination.
(3) experiment effect that extends the storage period of plant seed or fruit
embodiment 11
Extend fruits as the sugarcane experiment effect of storage period (the synthetic small peptide the present invention relates to: Tyr-Leu-Tyr-Glu-Val-Gly-Arg, Tyr-Leu-Tyr-Glu-Val-Val-Arg and Tyr-Leu-Tyr-Glu-Val-Ala-Arg and variant thereof, intercept 4 aminoacid sequence: Glu-Val-Ala-Arg; Intercept 5 aminoacid sequence: Tyr-Glu-Val-Ala-Arg; Intercept 6 aminoacid sequences: Leu-Tyr-Glu-Val-Ala-Arg)
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention (Tyr-Leu-Tyr-Glu-Val-Gly-Arg, Tyr-Leu-Tyr-Glu-Val-Val-Arg and Tyr-Leu-Tyr-Glu-Val-Ala-Arg and variant Glu-Val-Ala-Arg thereof, Tyr-Glu-Val-Ala-Arg, Leu-Tyr-Glu-Val-Ala-Arg), after HPLC purifying, purity is respectively 99%, 97.2%, 98.8%, 96.3%, 98% and 98.5%, be prepared into the small peptide solution that concentration is higher, be 10,50,100,500, the solution of 1000,1000 mg/ml.
Sugarcane: in sugarcane stem intercepting even thickness, the intercept of length 7 cm left and right, keeps cross section smooth, soaks 15 mins sterilizations with 5 % clorox.Then sugarcane with containing the aseptic small peptide solution soaking that the present invention relates to 1 hour, aseptic condition is preserved.Compare with the control group that the small peptide solution soaking not having through the present invention relates to is crossed, experimental group sugarcane stores the 10th talent generation browning phenomenon, than control group, has extended 6 days, and rate elongation can reach 30%～50%.The small peptide the present invention relates to has shown in the obvious prolongation effect accompanying drawing 4 of storage period (liquid prepared by small peptide of the present invention (Tyr-Leu-Tyr-Glu-Val-Ala-Arg)) sugarcane, wherein (1) experimental group sugarcane is to spend 1 hour with containing small peptide solution soaking of the present invention, and within the 9th day, color and luster is still constant; (2) control group is not use small peptide solution soaking of the present invention, clearly, and its complete brown stain.(variant of small peptide of the present invention intercepts liquid prepared by 4 amino acid (Glu-Val-Ala-Arg)) shown in accompanying drawing 5, wherein (1) experimental group sugarcane is to spend 1 hour with containing small peptide solution soaking of the present invention, within the 7th day, color and luster is still constant; (2) control group is not use small peptide solution soaking of the present invention, clearly, and its complete brown stain.
The small peptide effect of accompanying drawing 4 is more quite a lot of than the effect of its variant accompanying drawing 5.
for small peptide Tyr-Leu-Tyr-Glu-Val-Gly-Arg, Tyr-Leu-Tyr-Glu-Val-Val-Arg, obtained the experiment effect of similar Tyr-Leu-Tyr-Glu-Val-Ala-Arg.
for small peptide, intercept 5 aminoacid sequences (Tyr-Glu-Val-Ala-Arg), intercept the action effect that 6 aminoacid sequences (Leu-Tyr-Glu-Val-Ala-Arg) have been obtained 4 amino acid of similar intercepting (Glu-Val-Ala-Arg), its time that extends storage period, rate elongation increased thereupon along with forming the increase of the amino acid whose quantity of small peptide.
embodiment 12
Extend greengrocery---leaf vegetables as the effect experiment of the storage period of Chinese cabbage (the synthetic small peptide the present invention relates to:
Tyr-Leu-Tyr-Glu-Val-Ile-Arg）
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention (Tyr-Leu-Tyr-Glu-Val-Ile-Arg), after HPLC purifying, purity surpasses 98%, is prepared into the small peptide solution that concentration is higher, is the solution of 10 mg/ml.
At normal temperatures, Chinese cabbage is well placed, every layer of small peptide solution that spraying the present invention relates to, do not spray as a control group, experimental result shows, control group started there is jaundice at the 15th day, the phenomenon that comes off, and experimental group to the 20 talentes have yellow leaf phenomenon, and small peptide of the present invention obviously extends the storage period of Chinese cabbage.
embodiment 13
Extend fruit vegetables as cucumber, dish green pepper, fresh kidney beans, beet effect experiment (the synthetic small peptide the present invention relates to: Tyr-Leu-Tyr-Glu-Val-Leu-Arg) of storage period
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention (Tyr-Leu-Tyr-Glu-Val-Leu-Arg), after HPLC purifying, purity surpasses 97%, is prepared into the small peptide solution that concentration is higher, is the solution of 10 mg/ml.
Get cucumber, dish green pepper, fresh kidney beans, be bundled into bundle respectively, after the solution that the invention described above of spraying is respectively prepared into, be placed in plastics bag, experimental group is compared with the control group that does not spray the small peptide solution the present invention relates to, and extends the preservation period about 5 days.
embodiment 14
Extend the experiment of the melon effect as the storage period of watermelon (the synthetic small peptide the present invention relates to:
Tyr-Ile-Tyr-Glu-Val-Ala-Lys）
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention (Tyr-Ile-Tyr-Glu-Val-Ala-Lys), after HPLC purifying, purity surpasses 97%, is prepared into the small peptide solution that concentration is higher, is the solution of 10 mg/ml.
Get watermelon several, the small peptide solution the present invention relates in muskmelon pedicel position spraying, under the condition of hiding at sand, has the watermelon that is coated with small peptide solution prepared by the present invention at muskmelon pedicel position than many about 10 days of the freshness date that does not have to spray, and can reach the freshness date of 50 days.
embodiment 15
Extend berry fruit as effect experiment (the synthetic small peptide the present invention relates to: Tyr-Ile-Tyr-Glu-Val-Ala-Arg) of the storage period of strawberry, blueberry, blackberry, blueberry, grape
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention (Tyr-Ile-Tyr-Glu-Val-Ala-Arg), after HPLC purifying, purity surpasses 97%, is prepared into the small peptide solution that concentration is higher, is the solution of 10 mg/ml.
get strawberry, blueberry and blackberry, blueberry, the small peptide solution that spraying the present invention relates to, can extend the shelf-lives of 2 days, namely from the script shelf-lives of 5 days, can extend to the shelf-lives of 7 days, and small peptide of the present invention also has the effect of bacteria growing inhibiting.
Get grape enough, the small peptide solution that the present invention relates to of spraying, can extend grape storage period more than 3 days.
Extend walnut class as the experiment effect of the storage period of cherry, peach, date, longan, plum (the synthetic small peptide the present invention relates to: Tyr-Ile-Tyr-Glu-Gly-Gly-Lys)
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention (Tyr-Ile-Tyr-Glu-Gly-Gly-Lys), after HPLC purifying, purity surpasses 97%, is prepared into the small peptide solution that concentration is higher, is the solution of 10 mg/ml.
Get cherry 1000 of fruits well, good 100%, 500 of rate of fruit is placed on and contains in the small peptide solution the present invention relates to, liquid storage; 500 are placed in the solution that contains Sodium Benzoate food preservatives.After 40 days, with the cherry of the small peptide solution soaking the present invention relates to, good fruit rate is 98%, and the cherry steeping with Sodium Benzoate, good fruit rate is 89%.Clearly, observation directly perceived can be seen the difference between Fructus Pruni pseudocerasi, and this peptide obviously extends the storage period of cherry.
Same method processing peach, date, plum have obtained the similar experiment effect that extends storage period.
embodiment 17
Extend pomaceous fruit as the experiment effect of the storage period of apple, pears, loquat (the synthetic small peptide the present invention relates to: Tyr-Leu-Tyr-Glu-Val-Gly-Arg)
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention (Tyr-Leu-Tyr-Glu-Val-Gly-Arg), after HPLC purifying, purity surpasses 99%, is prepared into the small peptide solution that concentration is higher, is the solution of 10 mg/ml.
Select without interior wound, without the high quality apple of disease and pest, the solution that is placed in the small peptide preparation the present invention relates to soaks 5 minutes, pulls out, and places shady and cool place, and sweating 2 days, afterwards, packs freshness protection package into.The apple that the method was processed can reach more than 1 year storage period, apple extended pot life of processing than other modes 1～2 month.
Same method is processed pears, loquat has also obtained the effect that extends storage period.
embodiment 18
Extend flowers class as rose, carnation and the experiment effect at Cosmos bipinnatus florescence (the synthetic small peptide the present invention relates to: Tyr-Leu-Tyr-Glu-Val-Ala-Lys)
Adopt solid phase Fmoc method chemosynthesis small peptide of the present invention, after HPLC purifying, purity surpasses 96.3%, with physiological saline PBS, dissolves peptide of the present invention, is prepared into the solution of 10 mg/ml.
Get respectively Rose, carnation and Persian chrysanthemum, the above-mentioned solution of preparing with the small peptide the present invention relates to respectively, is sprayed onto and takes and cauline leaf place.The method can kind of blooming period prolonging 2～3 days.
Claims (10)
1. a small peptide that promotes plant establishment, germination, growth and prolongation storage period, is characterized in that: the aminoacid sequence that forms small peptide is as follows:
Tyr-Leu-Tyr-Glu-Val-Gly-Arg or
Tyr-Leu-Tyr-Glu-Val-Ala-Arg or
Tyr-Leu-Tyr-Glu-Val-Ile-Arg or
Tyr-Leu-Tyr-Glu-Val-Val-Arg or
Tyr-Leu-Tyr-Glu-Val-Leu-Arg or
Tyr-Ile-Tyr-Glu-Val-Ala-Arg or
Tyr-Ile-Tyr-Glu-Val-Ala-Lys or
Tyr-Ile-Tyr-Glu-Gly-Gly-Lys 。
2. the Nucleotide of small peptide according to claim 1 of encoding.
3. the small peptide fragment of the small peptide of a promotion plant establishment as claimed in claim 1, germination, growth and prolongation storage period, it is characterized in that: 4 or 5 or 6 amino acid form described small peptide fragment in order 7 described amino acid whose sequences are intercepted into from optional position, and there is the small peptide fragment that promotes plant germination, growth and prolongation storage period.
4. the small peptide fragment of the small peptide of a promotion plant establishment as claimed in claim 2, germination, growth and prolongation storage period, it is characterized in that: 4 or 5 or 6 base sequences corresponding to amino acid form the nucleotide fragments of described small peptide fragment in order the nucleotide sequence of 7 described amino acid whose correspondences is intercepted into from optional position, and there is the small peptide fragment of promotion plant establishment, germination, growth and prolongation storage period.
5. the analogue of the small peptide of a promotion plant establishment as claimed in claim 1, germination, growth and prolongation storage period, it is characterized in that: described small peptide analogue refers to and with described small peptide and another kind of compound, merging or merging other polypeptide or protein and form with the aminoacid sequence of described small peptide, and there is peptide sequence or the protein of promotion plant germination, growth and prolongation storage period.
6. the derivative of the small peptide of a promotion plant establishment as claimed in claim 1, germination, growth and prolongation storage period, it is characterized in that: after described small peptide derivative refers to amino acid whose one or several amino acid whose certain groups in the amino acid whose sequence of described small peptide are replaced with other group, and there is the small peptide that promotes plant germination, growth and prolongation storage period.
7. the variant of the small peptide of a promotion plant establishment as claimed in claim 1, germination, growth and prolongation storage period, it is characterized in that: described small peptide variant refers to a kind of nucleotide sequence that has the aminoacid sequence of one or several amino acid or Nucleotide change or encode it, described change is included in aminoacid sequence or nucleotide sequence, arbitrary topagnosis in the middle of sequence, insertion or replacement amino acid or Nucleotide, or at sequence two ends, add amino acid or Nucleotide, there are sequence homology or similarity with small peptide of the present invention.
8. the application of the small peptide of a promotion plant establishment as described in claim 1,2,3,4,5,6 or 7, germination, growth and prolongation storage period, it is characterized in that: described small peptide, Nucleotide, small peptide fragment, small peptide analogue, small peptide derivative and the preparation of small peptide variant have the biological functionality goods that promote that spermatophyte takes root, germinates, grows and extends storage period, and in low strength range, 0.01 mg/L～1 mg/L promotes plant establishment, germinating growth; At high concentration range 1 mg/L～1000 mg/L, suppressing growth is suitable for extending storage period.
9. application according to claim 8, is characterized in that: described biological functionality refers to that small peptide solution promotes phanerogamous take root, germinate, the promote growth of plant and the application function that extends the period of storage of plant seed or mature fruit etc. when high density when lower concentration
;described spermatophyte comprises angiosperm and gymnosperm.
10. application according to claim 8, is characterized in that: described spermatophyte is including but not limited to greengrocery, and described plant seed also comprises fruits, grain class, nuts and flowers class.
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| PCT/CN2014/090149 WO2015067146A1 (en) | 2013-11-05 | 2014-11-03 | Oligopeptide for promoting plant taking root, sprouting and growth, and for extending plant storage period, and application thereof |
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| WO2015067146A1 (en) * | 2013-11-05 | 2015-05-14 | 李�昊 | Oligopeptide for promoting plant taking root, sprouting and growth, and for extending plant storage period, and application thereof |
| CN105191991A (en) * | 2014-06-27 | 2015-12-30 | 哈尔滨归复记生物科技有限公司 | Biological seed and root soaking preparation and preparation method thereof |
| CN110423746A (en) * | 2019-07-16 | 2019-11-08 | 北京林业大学 | The method for encoding gene order, small peptide PFER and the application of small peptide PFER and inducing plant establishment |
| CN115073575A (en) * | 2022-06-29 | 2022-09-20 | 北京林业大学 | Multifunctional peptide for promoting woody plant cuttage survival and method for promoting cuttage rooting |
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| 张少权: "阿胶原料的主要蛋白组成及其生理活性的研究", 《中国优秀硕士学位论文全文数据库》, no. 8, 31 December 2005 (2005-12-31) * |
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| WO2015067146A1 (en) * | 2013-11-05 | 2015-05-14 | 李�昊 | Oligopeptide for promoting plant taking root, sprouting and growth, and for extending plant storage period, and application thereof |
| CN105191991A (en) * | 2014-06-27 | 2015-12-30 | 哈尔滨归复记生物科技有限公司 | Biological seed and root soaking preparation and preparation method thereof |
| CN110423746A (en) * | 2019-07-16 | 2019-11-08 | 北京林业大学 | The method for encoding gene order, small peptide PFER and the application of small peptide PFER and inducing plant establishment |
| CN115073575A (en) * | 2022-06-29 | 2022-09-20 | 北京林业大学 | Multifunctional peptide for promoting woody plant cuttage survival and method for promoting cuttage rooting |
| CN115073575B (en) * | 2022-06-29 | 2023-07-21 | 北京林业大学 | Multifunctional peptide for promoting cuttage survival of woody plants and method for promoting cuttage rooting |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2015067146A1 (en) | 2015-05-14 |
| CN103554236B (en) | 2015-06-17 |
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