KR102313318B1 - Long-term preservation hypochlorous acid water excellent in plant sterilization and growth promotion and its manufacturing method - Google Patents

Abstract

The present invention relates to hypochlorous acid water and a manufacturing method thereof, and more specifically, to hypochlorous acid water with excellent long-term storage, plant sterilization and plant growth-promoting properties and a manufacturing method thereof. A plant growth promoter according to the present invention comprises amino acids, hypochlorous acid water, a buffer having 6.0< pKa <8.5 at 25℃, and a bactericidal pH stabilizer. The plant growth promoter according to the present invention can control plant diseases of various plants and thus can effectively control the same. In addition, the plant growth promoter according to the present invention contains amino acids capable of being used by plants and thus is effective in promoting plant growth.

Description

Long-term preservation hypochlorous acid water excellent in plant sterilization and growth promotion and its manufacturing method

The present invention relates to hypochlorous acid water and a method for producing the same, and more particularly, to hypochlorous acid water excellent in long-term storage, plant sterilization and plant growth promoting properties and a method for producing the same.

Various compositions for promoting plant growth are provided. Korean Patent No. 1915782 discloses a composition for promoting plant growth containing natural organic matter, and Korean Patent No. 2006756 discloses a composition for promoting plant growth containing sulfur. Korean Patent No. 2132974 discloses a plant growth promoter made of a nano-particle porous carrier powder.

Korean Patent No. 10-1297712 discloses a method of promoting growth by controlling plants using hypochlorous acid water and soy protein. Soy protein content

However, there is still a continuing need for new plant growth promoters or compositions.

An object to be solved in the present invention is to provide a novel plant growth promoter.

Another object to be solved by the present invention is to provide a novel plant growth promoter capable of promoting plant growth while preventing pests and diseases.

In order to solve the above problems, the present invention

It provides a plant growth promoter comprising amino acids, hypochlorous acid water, a buffer of 6.0 < pKa < 8.5 at 25° C., and a bactericidal pH stabilizer.

In the present invention, the amino acid is an increase in chlorophyll production, a rich source of organic nitrogen, stimulation of vitamin synthesis, promotion of flowering, increase in fruiting, coloration of fruits, nutrition, size, flavor, increase in sugar content, and resistance to pests and pathogens included for Although not limited in theory, most plants have the ability to synthesize all of the amino acids they need by themselves, but since this is a process that requires a lot of energy, it promotes the growth of plants by supplying amino acids.

In the present invention, the amino acid is an L-type amino acid, for example, L-alanine (Alanine), L- arginine (Arginine), L- asparagine (Asparagine), L- aspartic acid (Aspartic acid), L- cysteine ( Cysteine), L-Glutamic acid, L-Glutamine, Glycine, L-Pistidine, L-Isoleucine, L-Leucine, L-Lysine ( Lysine), L-Methionine, L-Phenylalanine, L-Proline, L-Pyrrolysine, L-Selenocysteine, L-Serine, L- Threonine, L- tryptophan, L- tyrosine (Tyrosine), L- valine (Valin) may be.

In the present invention, the amino acids are free FORM (free amino acids) or short peptides (peptides), for example, dimers or trimers, or tetramers of amino acids so that the amino acids are easily absorbed through the roots or tissues. Preference is given to using dimeric amino acid oligomers, for example 5 to octamers. Most preferably, it is a monomer, and when the amino acids are combined in a long chain, the molecules are large enough to penetrate the plant tissue, so the efficiency is reduced.

In the present invention, the amino acid may be used in an amount of 0.1 to 10% by weight, preferably 0.5 to 5% by weight, and most preferably 1 to 4% by weight.

In the present invention, the hypochlorous acid water can prevent bacteria and pests by itself, so it is used to support the growth of plants due to stress reduction.

Although not limited by theory, hypochlorous acid

HClO ---> H ⁺ + ClO ^- (1)

OCl ^- + H ₂ O + 2e ^- ---> HCl + 2OH ^- (2)

The sterilization action of HClO is sterilized by the generator oxygen generated by the following reaction formula (3) to support the growth of plants.

HClO ---> HCl + [O] (3)

In the present invention, the hypochlorous acid water supports the growth of plants by preventing bacteria and pests, but effective chlorine is at a concentration of 100 ppm or less, for example, 1 to 100 A concentration of ppm may be used, preferably a concentration of 10 to 90 ppm or less, more preferably a concentration of 20 to 80 ppm or less, even more preferably a concentration of 30 to 70 ppm or less, most preferably 50 ppm or less. It is recommended to be used as a concentration. When the content or chlorine concentration of the hypochlorous acid is higher than the above range, the stress due to the death of bacteria and pests is reduced, but as the soil becomes excessively acidified, growth may be weakened in the long term, and the concentration of hypochlorous acid is 1 ppm or less In some cases, it may not affect plant growth.

In the present invention, the pH of the hypochlorous acid water may be 5.0 to 6.9, preferably the pH may be in the range of 5.0 to 6.0.

In the practice of the present invention, the hypochlorous acid water may constitute 95 to 98% by weight, more preferably 96 to 97% by weight.

In the present invention, the buffer is used to extend the storage period of hypochlorous acid, and it is preferable to use a buffer having 6.0<pKa<8.5 at 25°C. Here, 'a buffer with 6.0<pKa<8.5' is understood to mean that at least one of the pKa of the buffer is in the above range. For example, pKa may have pKa1, pKa2, or pKa3, meaning that one pKa is in the above range.

In the practice of the present invention, the buffer is a hypochlorous acid preservation buffer disclosed by Korean Patent No. 1925900, for example, 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane sulfonic acid, 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]ethanesulfonic acid, 3-morpholinopropane-1-sulfonic acid, 1,4-piperazinediethalsulfonic acid can be selected from This patent is hereby incorporated by reference.

In the practice of the present invention, the buffer may be used in an amount of 0.02 to 0.2% by weight. When the content of the buffer is small, long-term storage may be lowered.

In the present invention, the bactericidal pH stabilizer may be a buffer capable of stabilizing the pH while having bactericidal properties capable of removing bacteria harmful to plants, preferably glutaraldehyde, cocobenzyl dimethyl aluminum chloride (Coco benzyl Dimethyl Ammonium Chloride), or a combination thereof. Although not limited by theory, the bactericidal pH stabilizer is a non-oxidative disinfectant, and has the same or similar pKa to the oxidative disinfectant, hypochlorous acid, so it is combined together to increase the sterilization property and at the same time stabilize the pH, thereby enabling long-term storage.

In the practice of the present invention, the bactericidal pH stabilizer may be used in the range of 0.1 to 2.0% by weight, preferably 0.2 to 1.0% by weight based on the total weight.

The present invention in one aspect,

A plant growth promoter comprising 1-4 wt% of amino acids, 95-98 wt% of hypochlorous acid, 0.02-0.2 wt% of a buffer having 6.0 < pKa <8.5 at 25 °C, and 0.1-2.0 wt% of a bactericidal pH stabilizer provides

The present invention in one aspect,

It provides a plant disease control composition consisting of 1-4 wt% of amino acids, 95-98 wt% of hypochlorous acid, 0.02-0.2 wt% of a buffer having 6.0 < pKa <8.5 at 25 °C, and 0.1-2.0 wt% of a bactericidal pH stabilizer.

A novel plant growth promoter capable of controlling plant diseases and promoting plant growth according to the present invention is provided.

The plant growth promoter according to the present invention can control plant diseases of various plants, so it is possible to effectively control it, and since it contains amino acids that can be used by plants, it is effective in promoting plant growth.

1 is a view showing the tomato wilt disease control test results.
FIG. 2 is a test report for FIG. 1 .
3 is a view showing the results of the plate plating method for the test for inhibiting the growth of bacterial pore fungus in nuclear fruit.
4 is a view showing the results of the suspension preparation method for the test for inhibiting the growth of bacterial pore fungus in nuclear fruit.
5 is a test report for FIGS. 2 and 3 .
6 is a view showing the results of the banana anthrax control test results.
7 is a test report for FIG.
8 is a test report for various bacteria.
9 is a test report for corona virus.

Hereinafter, the present invention will be described in detail through examples.

Example 1. Tomato wilt disease control test

To determine whether hypochlorous acid water has bactericidal properties against tomato wilting bacteria (Fusarium oxysporum TF 104), a test was requested from the National Academy of Agricultural Sciences. Put spores (1x10 ⁶ spores/mL) of tomato wilting fungus ( Fusarium oxysporum TF 104) into sterile water and hypochlorous acid water, respectively, and mix them, and after 10 seconds, 60 seconds, and 180 seconds, streptomycin + PDA (potato agar medium) 3 times After plating, it was irradiated by culturing at 25° C. for 3 days. As shown in Figure 1, the hypochlorous acid water treatment group showed 100% sterilization power regardless of time.

Example 2. Test for inhibition of growth of nuclear fruit bacterial pore fungus

Using a non-acidic hypochlorous acid water undiluted solution, a test was requested to the National Institute of Horticultural and Herbal Science, the Agricultural Promotion Agency, to determine whether it has the effect of inhibiting the growth of the nuclear fruit bacterial pore fungus (Xanthomonas arboricola pv. pruni). The tests were conducted using the flat plating method and the suspension method.

For flat plate smearing, non-acidic hypochlorite water, one antibiotic (oxolinic acid, positive control), and untreated, bacteriostatic bacteria are smeared on LB solid medium, and a 5mm paper disk immersed in the treatment agent is smeared with bacteriostatic bacteria. After implantation in the LB solid medium, it was cultured in an incubator, and the growth inhibitory effect was assayed from the 2nd day of culture.

For the suspension preparation method, prepare a bacterial pore bacterial culture solution for non-acidic hypochlorous water, one antibiotic (oxolinic acid, positive control), and untreated, and mix the treatment agent with the bacterial pore bacterial culture solution 1:1 to prepare the suspension. After preparation, a portion of the mixed solution was smeared on LB solid medium, and the growth inhibitory effect was assayed from the second day of culture. 3 shows the results using the flat plating method, and FIG. 4 shows the results according to the suspension preparation method, respectively.

Example 3. Banana anthrax control test

A test was requested to the National Academy of Agricultural Sciences to determine whether hypochlorous acid water had bactericidal properties against banana anthrax (Colletotrichum musae KACC40947). Banana anthrax ( Csolletotrichum musae KACC40947) spores (1x10 ⁶ spores/mL) were put in sterile water and hypochlorous acid water, respectively, and mixed, and then smeared 3 times on streptomycin+PDA (potato agar medium) after 10 seconds, 60 seconds, and 180 seconds After that, it was irradiated by culturing at 25°C for 3 days. As shown in Figure 6, the hypochlorous acid water treatment group showed 100% sterilization power regardless of time.

Example 4. Sterilization test for various bacteria

Sterilization test on E. coil, S. aureus, B. cereus, K. pneumoniae, and P. aeruginosa was commissioned by KTR Korea Research Institute of Chemical Convergence and carried out. Conducted by the test method of ASTM E2315-15, the sterilization effect was found to be 90 ~ 99.999% log reduction. The results are shown in FIG. 8 .

Example 5. Antiviral test against coronavirus

In order to confirm the bactericidal activity against the corona virus (fCoV), a test was requested from the Korea Research Institute of Chemical Technology. As a result of the test, when the virus was reacted with 90% of non-acidic hypochlorous acid for 1 minute, virus reduction was observed, and after 3 minutes, the virus was reduced until it could no longer be observed due to cytotoxicity. The results are shown in FIG. 9 .

Example 6. Preparation of plant growth promoter

20 g of an amino acid mixture containing equal amounts of L-Aspartic acid, L-Cysteine, L-Glutamic acid, L-Glutamine, Glycine, L-Histidine, and L-Isoleucine, with an effective chlorine concentration of 50 ppm and pH 6.0 A plant growth promoter was prepared by mixing 970 g of hypochlorous acid water, 2 g of 3-morpholinopropane-1-sulfonic acid, and 8 g of cocobenzyl dimethyl aluminum chloride.

Comparative Example 1

A plant growth promoter was prepared by mixing 990 g of hypochlorous acid water having an effective chlorine concentration of 50 ppm, pH 6.0, 2 g of 3-morpholinopropane-1-sulfonic acid, and 8 g of cocobenzyl dimethyl aluminum chloride.

Comparative Example 2

20 g of an amino acid mixture containing equal amounts of L-Aspartic acid, L-Cysteine, L-Glutamic acid, L-Glutamine, Glycine, L-Histidine, and L-Isoleucine, with an effective chlorine concentration of 50 ppm and pH 6.0 A plant growth promoter was prepared by mixing 980 g of hypochlorous acid water.

Example 7. Tomato cultivation

After planting the plant growth promoter prepared in Example 6 in the facility, it was used in tomatoes grown after fertilizing 4 times at 25-day intervals. The growth effect was measured by measuring the average weight of a plant growth promoter sprayed with water in a weight ratio of 1:100 and water alone.

item Results (average weight) Use of Example 6 Plant Growth Promoter by Mixing with Water 210 g Using Comparative Example 1 mixed with water 165 g use water 150 g

Example 8. Tomato disaster prevention

After planting the plant growth promoters prepared in Example 6 and Comparative Example 1 in the facility, fertilized four times at 25-day intervals, respectively, and then used in the cultivated tomatoes. After infection with tomato wilting bacteria ( Fusarium oxysporum TF 104 ), the plant growth promoter of Example 1 was sprayed with water in a mixture in a weight ratio of 1:100, Comparative Example 1 was sprayed with water, and only water The growth effect was measured by measuring the average weight of those used.

item Results (average weight) 205 g of Example 6 plant growth promoter mixed with water Mixed with water, Comparative Example 1 using plant growth promoter 170 g use water Cultivation failure

Example 9. Tomato disaster prevention

The plant growth promoters of Example 6 and Comparative Example 2 were used in tomatoes grown after 12 months had elapsed, and then planted in a facility, fertilized 4 times at intervals of 25 days. After infection with tomato wilting fungus ( Fusarium oxysporum TF 104), the plant growth promoter of Example 6 and Comparative Example 2 were mixed with water in a weight ratio of 1:100 and sprayed with water and only water The average weight was measured The growth effect was measured.

item Results (average weight) Use of Example 6 Plant Growth Promoter by Mixing with Water 200 g Mixed with water and used as a plant growth promoter in Comparative Example 2 Cultivation failure use water Cultivation failure

As in Example 6, when amino acids and buffers were used, the control effect was high even after long-term storage, and the growth and development effect was excellent. On the other hand, the growth promoter without amino acids has controllability, but the growth effect due to control was weak, and in the absence of a buffer, the control effect was reduced when organs were preserved.

Claims (10)

A plant growth promoter consisting of 1-4 wt% of amino acids, 95-98 wt% of hypochlorous acid water, 0.02-0.2 wt% of a buffer having 6.0 < pKa <8.5 at 25 °C, and 0.1-2.0 wt% of a bactericidal pH stabilizer. According to claim 1,
The amino acid is a plant growth promoter, characterized in that the L-type amino acid. 3. The method of claim 1 or 2,
The amino acids are L-Alanine, L-Arginine, L-Asparagine, L-Aspartic acid, L-Cysteine, L-Glutamic acid, L-Glutamine, Glycine, L-Histidine, L-Isoleucine, L-Leucine, L-Lysine , L-Methionine, L-Phenylalanine, L-Proline, L-Pyrrolysine, L-Selenocysteine, L-Serine, L-Threonine, L-Tryptophan, L-Tyrosine, characterized in that at least one selected from the group consisting of L-Valin plant growth promoter. 3. The method of claim 1 or 2,
Plant growth promoter, characterized in that the amino acid is a monomer, a dimer, a trimer or an oligomer of less than 10 mers. delete The plant growth promoter according to claim 1, wherein the hypochlorous acid water has an effective chlorine concentration of 1 to 100 ppm. The plant growth promoter according to claim 1, wherein the hypochlorous acid water comprises 3-morpholinopropane-1-sulfonic acid, 1,4-piperazine diethalsulfonic acid, or a mixture thereof. The plant growth promoter according to claim 1, wherein the bactericidal pH stabilizer is a non-oxidizing bactericidal pH stabilizer. The plant growth promoter according to claim 1, wherein the bactericidal pH stabilizer is 3-morpholinopropane-1-sulfonic acid, cocobenzyl dimethyl aluminum chloride, or a combination thereof. A plant growth promoter consisting of 1-4 wt% of amino acids, 95-98 wt% of hypochlorous acid water, 0.02-0.2 wt% of a buffer with 6.0 < pKa <8.5 at 25 °C, and 0.1-2.0 wt% of a bactericidal pH stabilizer is sprayed onto plants. A method for controlling plants, characterized in that controlling pathogens.

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