CN106258079A - A kind of Fructus actinidiae chinensis seedling breeding method - Google Patents
A kind of Fructus actinidiae chinensis seedling breeding method Download PDFInfo
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- CN106258079A CN106258079A CN201610696762.9A CN201610696762A CN106258079A CN 106258079 A CN106258079 A CN 106258079A CN 201610696762 A CN201610696762 A CN 201610696762A CN 106258079 A CN106258079 A CN 106258079A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- Soil Sciences (AREA)
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a kind of Fructus actinidiae chinensis seedling breeding method, the present invention is by the control of the selection to melatonin concentration, low temperature stratification processing procedure, intermittent warming process and seedling medium, it is achieved that the high effect culture to Fructus actinidiae chinensis seedling.The present invention can make wild Chinensis Planch Seeds have germination percentage and the germinating energy of up to 90.09% of up to 91.80%, is significantly higher than existing conventional techniques；The present invention can make the survival rate of seedling be up to 99.8%.
Description
Technical field
The invention belongs to field of agricultural cultivation, be specifically related to a kind of Fructus actinidiae chinensis seedling breeding method.
Background technology
A.chinensis Planch. originates in China, more is in wild state, its fruit rich in vitamin C, have " Solanum muricatum ",
The laudatory title of " Prunus persica f. compressa ", is emerging cultivating apple trees.
Research currently for the seedling breeding technology of wild A.chinensis Planch. is the most relatively fewer.The Chinese Academy of Sciences plants
The Xu Benmei of thing institute have studied the impact that gibberellins germinates for Fructus actinidiae chinensis, but the germination percentage of its gained not to the utmost such as people
Meaning, and germinating energy and survival rate are not investigated.The Pang Xiangmei of Shandong Agricultural University have studied gibberellins and different seedbed equally
Substrate is for the impact of A.chinensis Planch. seedling breeding, but its income effect is poor.
Therefore, how to improve germination percentage, germinating energy and the survival rate in wild A.chinensis Planch. seedling breeding and become this
The technology that field is urgently to be resolved hurrily.
Summary of the invention
For the shortcoming of prior art, it is an object of the invention to provide a kind of Fructus actinidiae chinensis seedling breeding method, the party
Method comprises the steps:
(1) seed is gathered；
(2) gained seed is carried out disinfection；
(3) being placed on the filter paper with distilled water moistening by the seed after sterilization, the captan solution with 0.1% keeps planting
Son keeps seed moistening；
(4) at 4 DEG C, carry out step (3) gains sealing lucifuge cold preservation 2 months；
(5) step (4) gains are immersed in the melatonin solution of 50 μm ol/L, then carry out sealing lucifuge and process；
(6) step (5) gains being carried out intermittent warming 15 days, during process, every day processes 16h at 4 DEG C, then at 25 ±
1 DEG C of incubator processes 8h；
(7) step (6) gains are cultivated in 25 ± 1 DEG C of incubators to seed and all show money or valuables one carries unintentionally, period with 0.1% gram
Bacterium pellet solution makes seed keep moistening；
(8) seed showed money or valuables one carries unintentionally is broadcast by turfy soil, wet coconut husk and perlite 2:2:1 by volume ratio mix making
Seedlings nursing plate in, surface cover fine earth, water；Seedlings nursing plate is put into the culturing room of 25 ± 1 DEG C, and daylight time is disposed as 12h, often
It waters sooner or later in right amount, keeps mixed soil to moisten, and waters weekly the captan solution sterilization of a time 0.1%；
(9) continuing to be put into the culturing room of 25 ± 1 DEG C by step (8) gains, daylight time is disposed as 12h, and every day is early
Water spray, watered a water for 3 days, watered weekly 1 Hoagland pancebrin, within every 2 weeks, water 0.2% urea liquid and 0.2% evening
Potassium dihydrogen phosphate, keeps mixed soil to moisten.
Described Fructus actinidiae chinensis is wild A.chinensis Planch..
Preferably, in step (1), the method gathering seed is:
1) the wild Fruits of Actinidia Chinensis Planch of harvesting ripe, room temperature is deposited；
2) treat that fruit is abundant soft ripe, peeling put into spun yarn mesh bag stranding broken rub rotten；Water is eluriated, makes impurity and the drift of flat grain
Go out, leave seed；
3) seed is spread dry in the shade in dry ventilation；After drying, the seed of caking is rubbed scattered, load in valve bag, be placed in
It is dried at shady and cool standby.
Preferably, in step (2), utilizing the liquor natrii hypochloritis of 5% to carry out seed processing sterilization, the process time is 5
Minute.
Preferably, in step (5), the sealing lucifuge process time is 24 hours.
Preferably, the process time of step (7) is 25 days.
Preferably, in step (8), every Kong Fangsi seed in seedlings nursing plate.
Preferably, in step (8), the thickness of restrainted fine earth is 0.2cm.
Preferably, in step (9), during cultivation, using nutritive cube, the specification of nutritive cube is 23cm × 18cm, each nutrition
One Seedling of pot culture.
Beneficial effects of the present invention:
1, the present invention can make wild Chinensis Planch Seeds have the germination percentage and up to 90.09% of up to 91.80%
Germinating energy, is significantly higher than existing conventional techniques；
2, the present invention can make the survival rate of seedling be up to 99.8%.
Detailed description of the invention
Below by embodiment, the present invention is specifically described, it is necessary to it is pointed out here that be that following example are simply used
In the present invention is further detailed, it is impossible to be interpreted as limiting the scope of the invention, being skilled in technique of this field
Some nonessential improvement and adjustment that personnel are made according to foregoing invention content, still fall within protection scope of the present invention.
Embodiment 1
(1) seed is gathered:
1) the wild Fruits of Actinidia Chinensis Planch of harvesting ripe, room temperature is deposited；
2) treat that fruit is abundant soft ripe, peeling put into spun yarn mesh bag stranding broken rub rotten；Water is eluriated, makes impurity and the drift of flat grain
Go out, leave seed；
4) seed is spread dry in the shade in dry ventilation；After drying, the seed of caking is rubbed scattered, load in valve bag, be placed in
It is dried at shady and cool standby.
(2) soak Fructus actinidiae chinensis seed 5min with the liquor natrii hypochloritis of 5% to carry out disinfection, be cleaned multiple times with distilled water afterwards
Seed removes sodium hypochlorite；
(3) being placed on the filter paper with distilled water moistening by the seed after sterilization, the captan solution with 0.1% keeps planting
Son keeps seed moistening；
(4) at 4 DEG C, carry out step (3) gains sealing lucifuge cold preservation 2 months；
(5) step (4) gains are immersed in the melatonin solution of 50 μm ol/L, then carry out sealing lucifuge and process；
(6) step (5) gains are carried out intermittent warming 15 days, 4 DEG C of 6h, 25 ± 1 DEG C of incubator 8h；
(7) step (6) gains are cultivated in 25 ± 1 DEG C of incubators to seed and all show money or valuables one carries unintentionally, period with 0.1% gram
Bacterium pellet solution makes seed keep moistening；
(8) seed of about the 2mm that shows money or valuables one carries unintentionally is broadcast by turfy soil, wet coconut husk and the ratio of perlite 2:2:1 by volume
In the seedlings nursing plate that mixing is made, surface covers fine earth, waters；Seedlings nursing plate is put into the culturing room of 25 ± 1 DEG C, and daylight time is respectively provided with
For 12h, sooner or later water in right amount every day, keep mixed soil to moisten, water weekly the captan solution sterilization of a time 0.1%；
(9) continuing to be put into the culturing room of 25 ± 1 DEG C by step (8) gains, daylight time is disposed as 12h, and every day is early
Water spray, watered a water for 3 days, watered weekly 1 Hoagland pancebrin, within every 2 weeks, water 0.2% urea liquid and 0.2% evening
Potassium dihydrogen phosphate, keeps mixed soil to moisten.
Utilizing this method, gained germination percentage is 91.80%, and germinating energy is 90.09%, and seedling survival rate is 98%.
Experimental example 1
Low temperature and the melatonin impact on Fructus actinidiae chinensis seed germination
(1) soak Fructus actinidiae chinensis seed 5min with the liquor natrii hypochloritis of 5% to carry out disinfection, be cleaned multiple times with distilled water afterwards
Seed removes sodium hypochlorite.
(2) in the culture dish of a diameter of 9cm, place two layers of filter paper, moisten filter paper with distilled water, drive the gas under filter paper away
Bubble.
(3) being layered on the filter paper of culture dish by seed after sterilization, a culture dish, 100 seeds are put in each process.
(4) in 9 culture dishs, distilled water, 1 μm ol/L melatonin, 10 μm ol/L melatonin, 50 μm ol/L are added respectively
Melatonin, 100 μm ol/L melatonin, 200 μm ol/L melatonin, 300mg/L gibberellins, 650mg/L gibberellins and 1000mg/L
Gibberellins is to keep seed moistening.
(5) culture dish black plastic bag is wrapped put into 4 DEG C of cold preservations of refrigerator 15 days.
(6) transferring to 25 DEG C of incubators after show money or valuables one carries unintentionally to seed, period adds respective handling liquid respectively and keeps seed to moisten.
(7) observe statistics germination percentage every day, finally calculate germinating energy.
The different melatonin concentration impact on Fructus actinidiae chinensis germination percentage of table 1
Melatonin process solution all can promote Fructus actinidiae chinensis kind in 10 μm ol/L to 100 μm ol/L as can be seen from Table 1
Sub-rudiment, is up to 16% with the germination percentage of 50 μm ol/L, and is significantly higher than the process of other concentration；And 1 μm ol/L and 200 μ
Mol/L melatonin solution processes seed and does not all show money or valuables one carries unintentionally.Cold preservation Fructus actinidiae chinensis seed processes through the Gibberellins solution of 3 concentration levels
After all have and show money or valuables one carries unintentionally, the germination percentage that 650mg/L and 1000mg/L Gibberellins solution processes is of a relatively high, respectively 21% and 30%；
And the germination percentage that 300mg/L Gibberellins solution processes is only 15%, it is substantially less than the germination percentage of other two concentration.At distilled water
The matched group of reason is without rudiment.Totally, each germination percentage processed and germinating energy are the highest, as long as analyzing its reason when being cool stratification
Between the shortest, not up to chilling requirement, germination percentage and germinating energy are the highest, therefore the later stage test extend the cool stratification time to 2 months.
Table 2 variable concentrations melatonin (MT) and gibberellins (GA) process the impact on Fructus actinidiae chinensis seed germination
Experimental example 2 low temperature stratification, melatonin and the intermittent warming impact on Fructus actinidiae chinensis seed germination
1 cold stratification (the 1 first moon, terminate by the end of February)
(1) soak seed 5min with the liquor natrii hypochloritis of 5% to carry out disinfection, seed is cleaned multiple times with distilled water afterwards
Except sodium hypochlorite.
(2) in the culture dish of a diameter of 9cm, place two layers of filter paper, moisten filter paper with distilled water, drive the gas under filter paper away
Bubble.
(3) being put into by clean seed and spread out on the filter paper of culture dish, the captan solution adding 0.1% keeps seed to fit
Preferably humidity and do not have too many hydrops.
(4) culture dish plastic bag is wrapped, put into 4 DEG C of cold preservations of refrigerator 2 months, notice that moisturizing is degerming.
2 melatonin and intermittent warming (starting at the beginning of 3 months, terminate by the end of March)
(1) being selected gibberellins concentration by trial test result is 800mg/L and melatonin concentration is 50 μm ol/L.
(2) seed taking out low temperature stratification is divided into 4 groups, is separately immersed in distilled water, the Gibberellins solution of 800mg/L, 50 μ
In the melatonin solution of mol/L and the mixed solution containing 800mg/L gibberellins and 50 μm ol/L melatonin, use black plastic
Culture dish is packaged by bag, lucifuge 24h.
(6) seed that lucifuge processes is carried out intermittent warming 15 days, 4 DEG C of 6h, 25 ± 1 DEG C of incubator 8h.
(7), after intermittent warming terminates, black plastic bag is enclose culture dish and is put into 25 ± 1 DEG C of incubators and cultivates complete to seed
Portion shows money or valuables one carries unintentionally, about 25 days.
(8) observing every day and add the captan solution of 0.1% and make seed keep moistening and prevent bacteria breed, statistics is sent out
Bud rate, finally calculates germinating energy.
Design melatonin concentration for the treatment of is 50 μm ol/L, and gibberellin treatment concentration is 800mg/L.It addition, in order to improve Mi
The germination rate of monkey peach seeds, cool stratification time lengthening is 2 months, adds the intermittent warming of 4-25 DEG C after Stratificated treatment.
From table 3 it is observed that 1. the cold stratification of 2 months greatly increases germination rate.Even if using after lamination
Clear water processes the germination rate of Fructus actinidiae chinensis seed and also reaches 78%；2. again by 50 μm ol/L after cold stratification and intermittent warming
Melatonin processes can significantly increase seed germination rate, and the effect to improving germinating energy reaches the most notable.3. gibberellins and take off black
Element is jointly processed by compared with independent gibberellin treatment not having facilitation, reduces germination rate on the contrary.
Gibberellins or melatonin solution soaking 24h contribute to breaking Fructus actinidiae chinensis seed dormancy, stratification, and effect is better than list
Solely gibberellin treatment.
Table 3 wild Chinensis Planch Seeds germination percentage and germinating energy
The impact on seedling percent of experimental example 3 substrate
Three kinds of substrate (table 4) are employed during Fructus actinidiae chinensis seedling breeding.Result shows that turfy soil is than Nutrition Soil more
It is suitable for the growth of Fructus actinidiae chinensis seedling.Nutrition Soil: Vermiculitum: the substrate meta-alkalescence that perlite (3:1:1) mixes, pH 8～9, rudiment
True leaf dark green.But aetiolation, radicle brownization occurs in the rough leaf grown, part seedling leaves is wilted dead,
Mortality rate is 30.25%, illustrates that basic soil is not suitable for Fructus actinidiae chinensis growth of seedling.
Turfy soil: Vermiculitum: the substrate of perlite (4:1:1), soil pH=6～7, water-retaining property is preferable, but water penetration is poor.
Etiolated seedling moves in this substrate and recovers bottle green through 1～2 week blade, gradually grows immature side root.But owing to substrate is permeable
Property poor fibrous root poor growth, part seedling root brownization rot and dead, mortality rate is 36.51%.Watering many, root is the most rotten
Rotten；Watering few, substrate easily hardens.
Fructus actinidiae chinensis seedling immigration turfy soil after showing money or valuables one carries unintentionally: coconut husk (wet): in perlite=2:2:1 (volume ratio) substrate.
This matrix soil loosens, and breathability and water-retaining property are all preferable, and pH scope is 6 to 7, and seedling enters fast growing period, survival rate
To 99.80%, can fully absorb soil Middle nutrition, upgrowth situation is good.Illustrate that Fructus actinidiae chinensis seedling is suitably slant acidity, ventilative guarantor
The substrate of water grows.
The impact on Fructus actinidiae chinensis seedling percent of table 4 substrate
Claims (9)
1. a Fructus actinidiae chinensis seedling breeding method, it is characterised in that described method comprises the steps:
(1) seed is gathered；
(2) gained seed is carried out disinfection；
(3) being placed on the filter paper with distilled water moistening by the seed after sterilization, the captan solution with 0.1% keeps seed to protect
Hold seed moistening；
(4) at 4 DEG C, carry out step (3) gains sealing lucifuge cold preservation 2 months；
(5) step (4) gains are immersed in the melatonin solution of 50 μm ol/L, then carry out sealing lucifuge and process；
(6) step (5) gains being carried out intermittent warming 15 days, during process, every day processes 16h at 4 DEG C, then at 25 ± 1 DEG C
Incubator processes 8h；
(7) step (6) gains are cultivated in 25 ± 1 DEG C of incubators to seed and all show money or valuables one carries unintentionally, period with 0.1% captan
Solution makes seed keep moistening；
(8) seed showed money or valuables one carries unintentionally is broadcast educating of being made up of the ratio mixing of turfy soil, wet coconut husk and perlite 2:2:1 by volume
In Seedling dish, surface covers fine earth, waters；Seedlings nursing plate is put into the culturing room of 25 ± 1 DEG C, and daylight time is disposed as 12h, and every day is early
Evening waters in right amount, keeps mixed soil to moisten, and waters weekly the captan solution sterilization of a time 0.1%；
(9) continuing to be put into the culturing room of 25 ± 1 DEG C by step (8) gains, daylight time is disposed as 12h, the every day of spray sooner or later
Water, waters a water for 3 days, waters weekly 1 Hoagland pancebrin, within every 2 weeks, waters 0.2% urea liquid and 0.2% phosphoric acid
Potassium dihydrogen solution, keeps mixed soil to moisten.
Method the most according to claim 1, it is characterised in that described Fructus actinidiae chinensis is wild A.chinensis Planch..
Method the most according to claim 1, it is characterised in that in step (1), the method gathering seed is:
1) the wild Fruits of Actinidia Chinensis Planch of harvesting ripe, room temperature is deposited；
2) treat that fruit is abundant soft ripe, peeling put into spun yarn mesh bag stranding broken rub rotten；Water is eluriated, makes impurity and flat grain drift out,
Leave seed；
3) seed is spread dry in the shade in dry ventilation；After drying, the seed of caking is rubbed scattered, load in valve bag, be placed in dry
At Yin Liang standby.
Method the most according to claim 1, it is characterised in that in step (2), utilizes the liquor natrii hypochloritis of 5% to kind
Son carries out processing sterilization, and the process time is 5 minutes.
Method the most according to claim 1, it is characterised in that in step (5), the sealing lucifuge process time is 24 hours.
Method the most according to claim 1, it is characterised in that the process time of step (7) is 25 days.
Method the most according to claim 1, it is characterised in that in step (8), every Kong Fangsi seed in seedlings nursing plate.
Method the most according to claim 1, it is characterised in that in step (8), the thickness of restrainted fine earth is 0.2cm.
Method the most according to claim 1, it is characterised in that in step (9), during cultivation, uses nutritive cube, nutritive cube
Specification is 23cm × 18cm, one Seedling of each nutrition pot culture.
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| CN201610696762.9A CN106258079B (en) | 2016-08-19 | 2016-08-19 | A kind of Kiwi berry seedling breeding method |
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| CN201610696762.9A CN106258079B (en) | 2016-08-19 | 2016-08-19 | A kind of Kiwi berry seedling breeding method |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110574667A (en) * | 2019-09-24 | 2019-12-17 | 西北农林科技大学 | establishment method and application of kiwi plant sand culture technology system |
| CN113712033A (en) * | 2021-09-08 | 2021-11-30 | 河南科技学院 | Seed dressing agent for promoting peanut growth and early hair growth and preparation method thereof |
| CN114402983A (en) * | 2022-01-26 | 2022-04-29 | 台州学院 | Cultivation method of kiwi AM fungal mycorrhizal stock seedlings |
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| CN102771260A (en) * | 2012-07-05 | 2012-11-14 | 江苏省中国科学院植物研究所 | Collecting method of kiwi fruit seeds |
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| CN102771260A (en) * | 2012-07-05 | 2012-11-14 | 江苏省中国科学院植物研究所 | Collecting method of kiwi fruit seeds |
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|---|---|---|---|---|
| CN110574667A (en) * | 2019-09-24 | 2019-12-17 | 西北农林科技大学 | establishment method and application of kiwi plant sand culture technology system |
| CN113712033A (en) * | 2021-09-08 | 2021-11-30 | 河南科技学院 | Seed dressing agent for promoting peanut growth and early hair growth and preparation method thereof |
| CN113712033B (en) * | 2021-09-08 | 2023-01-17 | 河南科技学院 | Seed dressing agent for promoting peanut growth and early hair growth and preparation method thereof |
| CN114402983A (en) * | 2022-01-26 | 2022-04-29 | 台州学院 | Cultivation method of kiwi AM fungal mycorrhizal stock seedlings |
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