CN103931635B - Agriculturally useful compositions containing oligochitosan and toyokamycin and application - Google Patents
Agriculturally useful compositions containing oligochitosan and toyokamycin and application Download PDFInfo
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- CN103931635B CN103931635B CN201410180769.6A CN201410180769A CN103931635B CN 103931635 B CN103931635 B CN 103931635B CN 201410180769 A CN201410180769 A CN 201410180769A CN 103931635 B CN103931635 B CN 103931635B
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- 229920001661 Chitosan Polymers 0.000 title claims abstract description 36
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 24
- 201000010099 disease Diseases 0.000 claims abstract description 23
- 241000196324 Embryophyta Species 0.000 claims abstract description 18
- 230000008635 plant growth Effects 0.000 claims abstract description 5
- 230000001105 regulatory effect Effects 0.000 claims abstract description 5
- 241000700605 Viruses Species 0.000 claims abstract description 4
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 4
- 230000001580 bacterial effect Effects 0.000 claims abstract description 4
- 240000007594 Oryza sativa Species 0.000 claims abstract description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 3
- 241000918585 Pythium aphanidermatum Species 0.000 claims abstract description 3
- 208000036142 Viral infection Diseases 0.000 claims abstract description 3
- 235000009566 rice Nutrition 0.000 claims abstract description 3
- 238000005507 spraying Methods 0.000 claims abstract description 3
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- 238000012545 processing Methods 0.000 claims description 2
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- 239000004562 water dispersible granule Substances 0.000 claims description 2
- 239000004563 wettable powder Substances 0.000 claims description 2
- 238000007654 immersion Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 26
- 230000003449 preventive effect Effects 0.000 description 13
- 239000003814 drug Substances 0.000 description 8
- 230000003115 biocidal effect Effects 0.000 description 7
- 241000219109 Citrullus Species 0.000 description 6
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 6
- 239000000575 pesticide Substances 0.000 description 6
- 206010059866 Drug resistance Diseases 0.000 description 5
- 241000722363 Piper Species 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 244000221633 Brassica rapa subsp chinensis Species 0.000 description 4
- 241000227653 Lycopersicon Species 0.000 description 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000186361 Actinobacteria <class> Species 0.000 description 3
- 235000002566 Capsicum Nutrition 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 239000006002 Pepper Substances 0.000 description 3
- 235000016761 Piper aduncum Nutrition 0.000 description 3
- 235000017804 Piper guineense Nutrition 0.000 description 3
- 235000008184 Piper nigrum Nutrition 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 244000270200 Citrullus vulgaris Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- OMOVVBIIQSXZSZ-UHFFFAOYSA-N [6-(4-acetyloxy-5,9a-dimethyl-2,7-dioxo-4,5a,6,9-tetrahydro-3h-pyrano[3,4-b]oxepin-5-yl)-5-formyloxy-3-(furan-3-yl)-3a-methyl-7-methylidene-1a,2,3,4,5,6-hexahydroindeno[1,7a-b]oxiren-4-yl] 2-hydroxy-3-methylpentanoate Chemical compound CC12C(OC(=O)C(O)C(C)CC)C(OC=O)C(C3(C)C(CC(=O)OC4(C)COC(=O)CC43)OC(C)=O)C(=C)C32OC3CC1C=1C=COC=1 OMOVVBIIQSXZSZ-UHFFFAOYSA-N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
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- 238000002156 mixing Methods 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 208000031968 Cadaver Diseases 0.000 description 1
- 235000012840 Citrullus vulgaris Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
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- 241000018646 Pinus brutia Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 241001655322 Streptomycetales Species 0.000 description 1
- XOKJUSAYZUAMGJ-UHFFFAOYSA-N Toyocamycin Natural products C1=C(C#N)C=2C(N)=NC=NC=2N1C1OC(CO)C(O)C1O XOKJUSAYZUAMGJ-UHFFFAOYSA-N 0.000 description 1
- 238000001720 action spectrum Methods 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
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- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
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- 235000016709 nutrition Nutrition 0.000 description 1
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- 230000001737 promoting effect Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- XOKJUSAYZUAMGJ-WOUKDFQISA-N toyocamycin Chemical compound C1=C(C#N)C=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O XOKJUSAYZUAMGJ-WOUKDFQISA-N 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to agriculture field, relate to the compositions containing oligochitosan Yu toyokamycin, the mass fraction of oligochitosan and toyokamycin is than for 10:1-1:10. Compositions is with seed dressing, spraying, pouring root, impregnating application, for preventing and treating plant by virus, antibacterial, fungus-caused plant disease, preferred disease has droop, gray mold, virosis, bacterial wilt, damping-off, rice blast etc., and compositions also has the effect of good plant growth regulating.
Description
Technical field
The invention belongs to plant protection art, particularly relate to the agriculturally useful compositions containing oligochitosan and toyokamycin and application, the present composition can effectively prevent and treat corps diseases.
Background technology
In recent years, due to single and not Scientific Usage of Drugs, pest and disease damage has produced Drug resistance in various degree, and agriculture and forestry produce still with chemical pesticide for mainly preventing and treating kind, causes seriously increasingly destroying of environmental pollution and ecological environment. Enhancing along with the raising of people's living standard and health perception, develop and the exploitation of biological pesticide progressively receive the concern of pesticide R&D worker, become the focus of agriculture and forestry sustainable development, be the effective way improving environment, minimizing or delaying drug resistance and raising drug effect. Wherein, oligochitosan and antibiotic become one of focus of biological pesticide research.
Agricultural antibiotic, refer to by fermentable produce, there is pesticide function, for agriculturally preventing and treating the secondary metabolite of the harmful organisms such as Pest organism Mus. The microorganisms such as actinomycetes, fungus, antibacterial all can produce agricultural antibiotic, and the agricultural antibiotic that wherein actinomycetes produce is maximum. The many important agricultural antibiotic being widely used at present is all separate produced by the actinomycetes obtained from streptomyces. Agricultural antibiotic the earliest is streptomycin, and has thus promoted antibiotic to obtain rapid development after application agriculturally, 20 century 70s.
Plant rhizosphere is often referred to the distance micro-territory of root surface 1��4mm or more short-range soil. Due to organic existence such as secretions from plant roots, constituting a special microecological environment around rhizosphere, in this environment, abundant nutrition and the energy make microorganism all many than non-rhizospheric environment on value volume and range of product. Research shows, some rhizosphere microorganisms secretion metabolite can antagonism plant pathogenic microorganisms and coordinate plant growth, have become as an important sources of agricultural microorganism medicine.Rich adding streptomycete, fibrillae of spores pine is spacious and tight spiral, spore oval, surface band spinelet, produces toyokamycin (toyocamycin), it is suppressed that mycobacteria, yeast, filamentous fungi, plant disease fungus.
At present, but without the research report finding oligochitosan and the agriculturally useful compositions of toyokamycin.
Summary of the invention
It is an object of the invention to improve environment, expand action spectrum, minimizing or delaying drug resistance, raising drug effect, it is provided that the agriculturally useful compositions of a kind of oligochitosan and toyokamycin and application.
The present invention is achieved through the following technical solutions:
Compositions containing oligochitosan Yu toyokamycin, it is characterised in that: the mass fraction of oligochitosan and toyokamycin is than for 10:1-1:10. Preferably, the mass fraction of antibiotics and botanical components is than for 5:1-1:5.
The above-mentioned composition process preparation of the present invention is water preparation, suspending agent, wettable powder, water dispersible granules, and it is 0.05-80% that the quality sum of oligochitosan and toyokamycin accounts for the percentage ratio of preparation gross mass. Preferably, the quality sum of oligochitosan and toyokamycin accounts for the percentage ratio of preparation gross mass is 0.1-40%
The preparation of the above-mentioned composition processing of the present invention, with seed dressing, spraying, pouring root, impregnating application, shows the purposes preventing and treating various crop disease.
Present invention also offers the purposes for controlling plant diseases of the compositions containing oligochitosan and toyokamycin, the disease of suitable preventing and treating is by virus, antibacterial, fungus-caused plant disease, preferably, disease has droop, gray mold, virosis, bacterial wilt, damping-off, rice blast etc.
Present invention also offers the purposes for plant growth regulating of the compositions containing oligochitosan and toyokamycin, as short root length, short plant height, short chlorophyll keep, inspire bud, wound healing promoting etc.
The compositions of the present invention compared with prior art, produces following beneficial effect:
1, purposes is widened: compositions can prevent and treat multiple diseases and the coordinate plant growth of crops.
2, use cost is reduced: because compositions has raising preventive effect, preventing and treating spectrum increase, decrease Pesticide use number of times, reduce use cost.
3, Drug resistance is delayed: the component that oligochitosan and the compositions of toyokamycin are different mechanism of action is composite, and disease is not likely to produce Drug resistance.
4, environmental friendliness: people and animals and non-target organism toxicity are low, degraded is fast in the environment, and low-residual is free from environmental pollution.
Detailed description of the invention
Only it is used for described in example below explaining the present invention, for not necessarily departing from amendment, replacement or the improvement that present invention spirit is made with principle, belongs to the scope of protection of present invention.
The embodiment 1 agriculturally useful compositions containing oligochitosan and the toyokamycin effect to watermelon blight
According to scheme, preparation built agent and single dose, water preparation preparation, and carry out activity preliminary experiment. According to trial test, preparation is configured to the concentration needed. Before selecting, stubble watermelon blight morbidity field, arranges field uniformly. The preparation of medication different agents is uniformly dressed seed (concentration is with effective milligram gauge of every kilogram of seed with medicament), is sowed, Routine Management. Citrullus vulgaris plant diseases situation is investigated when disease onset. Use Abbot formula, convert percentage rate to preventive effect.
In theoretical effect, x is concentration is the effect of substance A during a, and y is concentration is the effect of substance B during b, compares theoretical effect and actual effect, evaluates the synergism of compositions, with actual preventive effect more than theory preventive effect time for potentiation.Compositions to the result of Citrullus vulgaris disease in Table 1.
The table 1 agriculturally useful compositions containing oligochitosan and the toyokamycin effect to watermelon blight
By the test to watermelon blight of the agriculturally useful compositions containing oligochitosan and toyokamycin, as seen from Table 1, the compositions of oligochitosan and toyokamycin is to theoretical preventive effect higher than correspondent composition of the actual effect of watermelon blight. It is clear that watermelon blight is potentiation by the compositions of oligochitosan and toyokamycin.
The embodiment 2 agriculturally useful compositions containing oligochitosan and the toyokamycin prevention effect to graw mold of tomato
It is mixed into compost with sterilized soil and sterilizing river sand, fully after mixing, is mixed into medicament. Drug concentration contains the milligram number of the effective ingredient of this medicament in every kilogram of soil, directly mixes soil with quantitative suspension formulation accordingly, adds clear water and makes water content 30%, then mix rear quantitatively loading basin alms bowl thoroughly after fully mixing thoroughly. Fructus Lycopersici esculenti is planted in basin alms bowl, Routine Management. The percentage rate that plant blade face gray mold infects is measured when blank gray mold generally occurs. Use Abbot formula, convert percentage rate to preventive effect.
X is working concentration is the preventive effect of active component A during a, and y is working concentration is the preventive effect of active component B during b. Relatively theoretical effect and actual effect, evaluates the synergism of compositions, with actual preventive effect more than theory preventive effect time for potentiation. Result is in Table 2.
The table 2 agriculturally useful compositions containing oligochitosan and the toyokamycin prevention effect to graw mold of tomato
By potted plant test, as seen from Table 2, the actual preventive effect of graw mold of tomato is above the theoretical preventive effect of compositions by the compositions of oligochitosan and toyokamycin. It is clear that graw mold of tomato is potentiation by the compositions of oligochitosan and toyokamycin.
The embodiment 3 agriculturally useful compositions containing oligochitosan and toyokamycin is to pepper diseases effect
According to scheme, preparation built agent and unit dose preparation, and carry out activity preliminary experiment. According to determination of activity trial test, preparation is configured to the concentration needed. Setting corresponding single dose and blank before test in Fructus Capsici field, adopt manual sprayer routine conventional spray, every medicament sprays 50 strains. 7-10d dispenser 1 time again after 1st dispenser. 10-20d after 2nd dispenser, investigates pepper plant disease incidence of listing. Use Abbot formula, convert percentage rate to preventive effect.
In theoretical effect, x is concentration is the effect of substance A during a, and y is concentration is the effect of substance B during b, compares theoretical effect and actual effect, evaluates the synergism of compositions. Compositions to the result of pepper plant disease respectively in Table 3.
The table 3 agriculturally useful compositions containing oligochitosan and the toyokamycin field efficacy to pepper diseases
As seen from Table 3, the actual effect of pepper virus disease, gray mold and bacterial wilt is above theoretical effect by the agriculturally useful compositions containing oligochitosan Yu toyokamycin, it was shown that compositions has obvious synergistic function.
The embodiment 4 agriculturally useful compositions containing oligochitosan and the toyokamycin growth regulating effect to plant
Accurately measuring compositions and unit dose preparation respectively, be added in the container containing a small amount of tap water, adding water to water gross weight is 1000g, adds a cover, and covers the aperture of uniformity. Being dipped in culture fluid by the root of ready Brassica campestris L. ssp.chinensis Seedling by container cap bore, be put in culturing room and observe cultivate, every chemicals treatment repeats for 10 times. The plant height of regular inquiry Brassica campestris L. ssp.chinensis, root length, leaf color, result is in Table 4.
The table 4 agriculturally useful compositions containing oligochitosan and toyokamycin effect on Brassica campestris L. ssp.chinensis
Brassica campestris L. ssp.chinensis is cultivated with the agriculturally useful compositions containing oligochitosan with toyokamycin, result is in Table 4, under same amount, plant height, root length, leaf color are better than oligochitosan and the effect of toyokamycin single dose by the agriculturally useful compositions of oligochitosan and toyokamycin, and the growth regulating of plant is had synergistic effect by the compositions of oligochitosan and toyokamycin.
Claims (7)
1. contain the compositions of oligochitosan and toyokamycin, it is characterised in that: the mass fraction of oligochitosan and toyokamycin is than for 5:1-1:4.
2. compositions according to claim 1, it is characterised in that the preparation of compositions processing is water preparation, suspending agent, wettable powder, water dispersible granules.
3. compositions according to claim 2, it is characterised in that it is 0.05-80% that the quality sum of oligochitosan and toyokamycin accounts for the percentage ratio of preparation gross mass.
4. compositions according to claim 3, it is characterised in that percentage ratio is 0.1-40%.
5. compositions according to claim 4, it is characterised in that compositions is with seed dressing, spraying, pouring root, immersion applications.
6. the compositions of claim 5 is for the purposes of controlling plant diseases, and the disease of suitable preventing and treating is that disease has droop, gray mold, virosis, bacterial wilt, damping-off, rice blast by virus, antibacterial, fungus-caused plant disease.
7. the compositions of claim 5 is for the purposes of plant growth regulating.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410180769.6A CN103931635B (en) | 2014-05-03 | 2014-05-03 | Agriculturally useful compositions containing oligochitosan and toyokamycin and application |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410180769.6A CN103931635B (en) | 2014-05-03 | 2014-05-03 | Agriculturally useful compositions containing oligochitosan and toyokamycin and application |
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| CN103931635A CN103931635A (en) | 2014-07-23 |
| CN103931635B true CN103931635B (en) | 2016-06-08 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108142247A (en) * | 2017-12-23 | 2018-06-12 | 青岛麦迪尔生物工程有限公司 | A kind of biological and ecological methods to prevent plant disease, pests, and erosion culture medium for cultivating matter of anti-graw mold of tomato |
| CN113951279A (en) * | 2021-11-04 | 2022-01-21 | 河南科技大学 | Bacteriostatic combination liquid for preventing and treating tobacco bacterial wilt as well as preparation method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3116222A (en) * | 1962-10-05 | 1963-12-31 | Pfizer & Co C | Process for preparing toyocamycin |
| CN101816305A (en) * | 2010-05-09 | 2010-09-01 | 海南正业中农高科股份有限公司 | Bactericide pesticide composite containing oligochitosan |
| CN101911944A (en) * | 2010-09-01 | 2010-12-15 | 北京三浦百草绿色植物制剂有限公司 | Pesticide composition for preventing and controlling plant nematode diseases and application thereof |
| CN101961013A (en) * | 2010-03-19 | 2011-02-02 | 中国计量学院 | Application of toyocamycin to controlling tomato gray mold |
-
2014
- 2014-05-03 CN CN201410180769.6A patent/CN103931635B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3116222A (en) * | 1962-10-05 | 1963-12-31 | Pfizer & Co C | Process for preparing toyocamycin |
| CN101961013A (en) * | 2010-03-19 | 2011-02-02 | 中国计量学院 | Application of toyocamycin to controlling tomato gray mold |
| CN101816305A (en) * | 2010-05-09 | 2010-09-01 | 海南正业中农高科股份有限公司 | Bactericide pesticide composite containing oligochitosan |
| CN101911944A (en) * | 2010-09-01 | 2010-12-15 | 北京三浦百草绿色植物制剂有限公司 | Pesticide composition for preventing and controlling plant nematode diseases and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 具有农业生物活性的壳寡糖的研究进展;刘幸海 等;《农药学学报》;20060330;第8卷(第1期);参见第1-7页 * |
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Denomination of invention: Agricultural composition containing chitosan oligosaccharide and fengjiamycin and its application Effective date of registration: 20220304 Granted publication date: 20160608 Pledgee: Bank of Hainan Limited by Share Ltd. Pledgor: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. Registration number: 2019460000007 |
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Address after: Fuchang Village, Laocheng Development Zone, Chengmai County, Hainan Province, 571923 Patentee after: Hainan Zhengye Biotechnology Co.,Ltd. Address before: 571924 Fuchang village, Laocheng Development Zone, Chengmai County, Hainan Province Patentee before: HAINAN ZHENGYE ZHONGNONG HIGH TECHNOLOGY Co.,Ltd. |
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