A kind of microbial bacterial agent and preparation method of prevention root disease
Technical field
The present invention relates to a kind of disease resistance microbial bacterial agents, belong to agricultural disease prevention and fertilizer trade field.Specifically
A kind of microbial bacterial agent and preparation method of prevention root disease.
Background technology
One of an important factor for plant disease is restriction crops good quality and high output.It is estimated that global staple crops is flat
Loss accounts for about 10%-the 15% of total output, and annual direct economic loss is up to hundreds billion of dollars.Plant disease is not only direct
Cause crop yield to decline reduces with quality, and it is a variety of can to secrete generation during infection crops for fraction of pathogens bacterium
To the harmful toxin of people and animals and metabolin, great threat is constituted to the safety of agricultural product.Make disease various, object root disease
Evil is always to perplex the headache of peasant, the generation of root disease will directly contribute it is serious be short of seedling, dead seedling in blocks, seedling not
The problems such as length, uneven emergence, crop yield is directly affected, heavy economic losses is caused.
The control of root disease often relies on chemical prevention, and insecticide and using for fungicide have made part pathogenic bacteria
Generate drug resistance so that peasant has to the dosage for increasing insecticide and fungicide, this inevitably bring environmental pollution with
Practice for Pesticide Residue in Agricultural Products problem.
It is preferable method currently, carrying out biological control to it using microorganism and its metabolite.It is anti-to can be used for biology
The microorganism controlled has fungi, bacterium, actinomyces etc..The Biocontrol Mechanism of biocontrol microorganisms is different, mainly has Competition, antagonism to make
With, induction crop resistance and promote plant growth, indirectly improve crop resistance the effects that, many Biocontrol microorganisms can also be by several
Combining to function between kind different mechanisms.
Invention content
The present invention in view of the above technical problems, provides a kind of microbial bacterial agent and preparation method of prevention root disease.
The purpose of the present invention is what is be achieved through the following technical solutions:
The present invention provides a kind of microbial bacterial agents of prevention root disease, include each component of following mass fraction:
Preferably, the preparation method of the subcritical hydrolysis product includes:Stalk, cow dung, chicken manure, agaric bacteria residue are mixed
It closes, the liquid of gained, as subcritical hydrolysis product after being separated by solid-liquid separation after subcritical hydrolysis reacts.Patent ZL can be used
Method and apparatus described in 2010 1 0580486.2 prepares the subcritical hydrolysis product.
Preferably, the stalk, cow dung, chicken manure, agaric bacteria residue parts by weight be respectively:20-40 parts, 10-40 parts,
10-30 parts, 10-30 parts.
Preferably, the microbial inoculum includes bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, gel-shaped bud
Spore bacillus, trichoderma viride.
Preferably, it is 2-10 that the microbial inoculum, which includes parts by weight ratio,:3-15:2-10:1-5:The bacillus subtilis of 1-5
Bacterium, bacillus amyloliquefaciens, bacillus licheniformis, bacillusmusilaginosiengineering, trichoderma viride.The weight part ratio of each bacterium
When being worth in the range, best control effect can reach.
Preferably, the bacillus subtilis, bacillus amyloliquefaciens living bacteria count be 50,000,000,000/gram, lichens bud
Spore bacillus, bacillusmusilaginosiengineering living bacteria count be 100,000,000,000/gram, the living bacteria count of trichoderma viride is 200,000,000/
Gram.
The present invention also provides a kind of preparation methods of the microbial bacterial agent of prevention root disease, include the following steps:
A, subcritical hydrolysis product is fermented after mixing with microbial inoculum;
B, potassium fulvate, algae essence, molasses powder and stabilizer are added in the zymotic fluid that step A is obtained, then ferment to get
The microbial bacterial agent.
Preferably, in step A, the parameter of the ferment control includes:Control 28-32 DEG C of tank temperature, ventilatory capacity 2-5m3/ h and
Tank presses 0.01-0.05Mpa, primary every 45min stirrings, and speed of agitator 100-150rpm stirs 10-20min, ferments 4-6 days.
Preferably, in step B, the parameter of the ferment control includes:Control 28-32 DEG C of tank temperature, ventilatory capacity 2-5m3/ h and
Tank presses 0.01-0.05Mpa, primary every 90min stirrings, and speed of agitator 100-150rpm stirs 15-30min, ferments 2-3 days.
The present invention also provides a kind of application method of the microbial bacterial agent of prevention root disease, the application method packets
It includes:Using punching apply or pouring root by the way of, usage amount is 5-50L per acre.
Compared with prior art, the present invention has following advantageous effect:
1, the high activity small molecule organic compound for the subcritical hydrolysis product that the present invention uses can enhance plant resistance to environment stress, use
Five kinds of biocontrol microorganisms can play good synergistic effect, improve crop resistance.
What 2, the present invention prepared microbial bacterial agent can effectively prevent plant root disease, especially to the treatment of droop and
Root knot prevents, and for the cure rate of droop up to 100%, the incidence of root knot can be reduced to 9%, has very outstanding anti-
Effect is controlled, can be widely applied in agricultural production.
Specific implementation mode
With reference to specific embodiment, the present invention is described in detail.Following embodiment will be helpful to the technology of this field
Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill of this field
For personnel, without departing from the inventive concept of the premise, several changes and improvements can also be made.These belong to the present invention
Protection domain.
Embodiment 1
Pear tree droop application effect
It is chosen in Henan Province Ningling County stone bridge town and has generated the pear tree of pear tree droop disease, 10 are one group, totally 4
Group, every irrigation amount are that microbial bacterial agent 5L converts 100kg water, dig annular ditch on the inside of each tree ring tree crown and pour 2 times.
The formula for the microbial bacterial agent that specific experiment processing uses is as shown in table 1, and the preparation method comprises the following steps:
Subcritical hydrolysis product is transferred in liquid fermentation tank after mixing with microbial inoculum, 28-32 DEG C of control tank temperature, ventilation
It measures 2-5m3/h and tank presses 0.01-0.05Mpa, primary every 45min stirrings, rotating speed 100-150rpm stirs 10-20min, hair
Ferment 5 days.Gained mixes object and accesses potassium fulvate, algae essence, molasses powder and stabilizer, 28-32 DEG C of control tank temperature, ventilatory capacity in proportion
2-5m3/h and tank press 0.01-0.05Mpa, primary every 90min stirrings, and rotating speed 100-150rpm stirs 15-30min, fermentation 2
It both obtains microbial bacterial agent.
The subcritical hydrolysis product is 20-40 parts of stalk, 10-40 parts of cow dung, 10-30 parts of chicken manure, the wood of parts by weight
The product liquid of ear bacteria residue 10-30 parts of solids and liquid resulting separation after subcritical hydrolysis reacts;
The microbial inoculum is bacillus subtilis, bacillus amyloliquefaciens, bacillus licheniformis, bacillusmusilaginosiengineering, green
Color Trichoderma, parts by weight ratio are 2-10:3-15::2-10:1-5:1-5.Bacillus subtilis, bacillus amyloliquefaciens
For 50,000,000,000/gram, bacillus licheniformis, bacillusmusilaginosiengineering be 100,000,000,000/gram, trichoderma viride is 200,000,000/gram.
Table 1
Specific experiment result is as shown in table 2 below.
Table 2
Serial number |
Cure rate |
Processing 1 |
100% |
Processing 2 |
50% |
Processing 3 |
70% |
Processing 4 |
60% |
Processing 5 |
80% |
Processing 6 |
0% |
After the processing of the microbial inoculum of the present invention, Pear Diseases symptom disappears, and branches and leaves are acted normally, and fruiting is normal.Remaining processing
With certain therapeutic effect.
Embodiment 2
Peach crown gall control effect
6 mu of honey peach are chosen in Shanghai curry favour Nan Zhen within 2016 to be tested, irrigation amount is that microbial bacterial agent 15L is converted per acre
1000kg water pours once, pours 3 times altogether, is primary before rudiment is bloomed respectively, young fruit period is primary, and fruit expanding period is primary.
The formula for the microbial bacterial agent that specific experiment processing uses is as shown in table 3, the preparation method and 1 phase of embodiment
Together.
Table 3
Serial number |
Subcritical hydrolysis product |
Microbial inoculum |
Potassium fulvate |
Algae essence |
Molasses powder |
Stabilizer |
Processing 1 |
72 |
8 |
8 |
8 |
3 |
1 |
Processing 2 |
82 |
0 |
6 |
7 |
4 |
1 |
Processing 3 |
74 |
12 |
0 |
10 |
3 |
1 |
Processing 4 |
76 |
11 |
9 |
0 |
3 |
1 |
Processing 5 |
63 |
15 |
15 |
6 |
0 |
1 |
Processing 6 |
— |
— |
— |
— |
— |
— |
Specific experiment result is as shown in table 4 below.
Table 4
After the processing of the microbial inoculum of the present invention, Peach crown gall incidence is substantially reduced, and peach quality improves, to a certain extent
Improve the economic benefit of orchard worker.
Embodiment 3-5
A kind of microbial bacterial agent is present embodiments provided, each component and content are as shown in table 5, preparation method and implementation
Example 1 is identical.
Table 5
Microbial bacterial agent prepared by embodiment 3-5 is used for the treatment of pear tree droop, application method and 1 phase of embodiment
Together.The results are shown in Table 6.After the processing of the microbial inoculum of embodiment 3-5, Pear Diseases symptom disappears substantially, and branches and leaves are acted normally, and hangs
Fruit is normal.
Table 6
Experimental group |
Cure rate |
Embodiment 3 |
98% |
Embodiment 4 |
95% |
Embodiment 5 |
92% |
Comparative example 1
This comparative example provides a kind of microbial bacterial agent, each component and content and the processing 1 of embodiment 1 is essentially identical,
It the difference is that only:In this comparative example, in the preparation of the subcritical hydrolysis product of use, raw material does not contain agaric bacteria residue.
Comparative example 2
This comparative example provides a kind of microbial bacterial agent, each component and content and the processing 1 of embodiment 1 is essentially identical,
It the difference is that only:In this comparative example, in the preparation of the subcritical hydrolysis product of use, raw material cow dung is replaced using pig manure.
Comparative example 3
This comparative example provides a kind of microbial bacterial agent, each component and content and the processing 1 of embodiment 1 is essentially identical,
It the difference is that only:In this comparative example, the microbial inoculum of use does not contain bacillus licheniformis.
Comparative example 4
This comparative example provides a kind of microbial bacterial agent, each component and content and the processing 1 of embodiment 1 is essentially identical,
It the difference is that only:In this comparative example, in the microbial inoculum of use, bacillusmusilaginosiengineering is replaced using bacillus laterosporus.
Microbial bacterial agent prepared by comparative example 1-4 is used for the treatment of pear tree droop, application method and 1 phase of embodiment
Together.The results are shown in Table 7.By contrast after the microbial inoculum processing of example 1-4, Pear Diseases symptom does not completely disappear, there is certain treatment effect
Fruit.
Table 7
Experimental group |
Cure rate |
Comparative example 1 |
85% |
Comparative example 2 |
87% |
Comparative example 3 |
82% |
Comparative example 4 |
83% |
Specific embodiments of the present invention are described above.It is to be appreciated that the invention is not limited in above-mentioned
Particular implementation, those skilled in the art can make a variety of changes or change within the scope of the claims, this not shadow
Ring the substantive content of the present invention.In the absence of conflict, the feature in embodiments herein and embodiment can arbitrary phase
Mutually combination.