CN110583698B - Microbial agent for preventing and treating crop root diseases and application - Google Patents
Microbial agent for preventing and treating crop root diseases and application Download PDFInfo
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- CN110583698B CN110583698B CN201911004481.2A CN201911004481A CN110583698B CN 110583698 B CN110583698 B CN 110583698B CN 201911004481 A CN201911004481 A CN 201911004481A CN 110583698 B CN110583698 B CN 110583698B
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 46
- 201000010099 disease Diseases 0.000 title claims abstract description 24
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 24
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 41
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 22
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 22
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 22
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 21
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 21
- 241000589540 Pseudomonas fluorescens Species 0.000 claims abstract description 20
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 14
- 239000003963 antioxidant agent Substances 0.000 claims abstract description 13
- 230000003078 antioxidant effect Effects 0.000 claims abstract description 13
- 239000003381 stabilizer Substances 0.000 claims abstract description 13
- 235000002566 Capsicum Nutrition 0.000 claims abstract description 10
- 240000008067 Cucumis sativus Species 0.000 claims abstract description 10
- 235000016623 Fragaria vesca Nutrition 0.000 claims abstract description 10
- 235000011363 Fragaria x ananassa Nutrition 0.000 claims abstract description 10
- 244000068988 Glycine max Species 0.000 claims abstract description 10
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 10
- 239000006002 Pepper Substances 0.000 claims abstract description 10
- 235000016761 Piper aduncum Nutrition 0.000 claims abstract description 10
- 235000017804 Piper guineense Nutrition 0.000 claims abstract description 10
- 235000008184 Piper nigrum Nutrition 0.000 claims abstract description 10
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims abstract description 9
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims abstract description 9
- 241000223218 Fusarium Species 0.000 claims abstract description 9
- 230000001580 bacterial effect Effects 0.000 claims abstract description 8
- 240000009088 Fragaria x ananassa Species 0.000 claims abstract 2
- 244000203593 Piper nigrum Species 0.000 claims abstract 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- ZTHYODDOHIVTJV-UHFFFAOYSA-N Propyl gallate Chemical compound CCCOC(=O)C1=CC(O)=C(O)C(O)=C1 ZTHYODDOHIVTJV-UHFFFAOYSA-N 0.000 claims description 8
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 4
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 4
- BGNXCDMCOKJUMV-UHFFFAOYSA-N Tert-Butylhydroquinone Chemical compound CC(C)(C)C1=CC(O)=CC=C1O BGNXCDMCOKJUMV-UHFFFAOYSA-N 0.000 claims description 4
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 4
- 235000010354 butylated hydroxytoluene Nutrition 0.000 claims description 4
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 claims description 4
- 239000001630 malic acid Substances 0.000 claims description 4
- 235000011090 malic acid Nutrition 0.000 claims description 4
- 239000000473 propyl gallate Substances 0.000 claims description 4
- 235000010388 propyl gallate Nutrition 0.000 claims description 4
- 229940075579 propyl gallate Drugs 0.000 claims description 4
- 235000010199 sorbic acid Nutrition 0.000 claims description 4
- 239000004334 sorbic acid Substances 0.000 claims description 4
- 229940075582 sorbic acid Drugs 0.000 claims description 4
- 239000011975 tartaric acid Substances 0.000 claims description 4
- 235000002906 tartaric acid Nutrition 0.000 claims description 4
- 239000004250 tert-Butylhydroquinone Substances 0.000 claims description 4
- 235000019281 tert-butylhydroquinone Nutrition 0.000 claims description 4
- SPSPIUSUWPLVKD-UHFFFAOYSA-N 2,3-dibutyl-6-methylphenol Chemical compound CCCCC1=CC=C(C)C(O)=C1CCCC SPSPIUSUWPLVKD-UHFFFAOYSA-N 0.000 claims description 3
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 3
- 235000015165 citric acid Nutrition 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims 2
- 241000894006 Bacteria Species 0.000 claims 1
- 241000223208 Curvularia Species 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 17
- 230000002265 prevention Effects 0.000 abstract description 9
- 230000002262 irrigation Effects 0.000 description 11
- 238000003973 irrigation Methods 0.000 description 11
- 238000002360 preparation method Methods 0.000 description 10
- 241000220223 Fragaria Species 0.000 description 8
- 241000722363 Piper Species 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 241000006381 Bacillus flexus Species 0.000 description 4
- 241000589876 Campylobacter Species 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 244000052769 pathogen Species 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 239000003899 bactericide agent Substances 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000004322 Butylated hydroxytoluene Substances 0.000 description 1
- NLZUEZXRPGMBCV-UHFFFAOYSA-N Butylhydroxytoluene Chemical compound CC1=CC(C(C)(C)C)=C(O)C(C(C)(C)C)=C1 NLZUEZXRPGMBCV-UHFFFAOYSA-N 0.000 description 1
- 235000009849 Cucumis sativus Nutrition 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000003181 biological factor Substances 0.000 description 1
- 229940095259 butylated hydroxytoluene Drugs 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000007084 physiological dysfunction Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
Abstract
The invention provides a microbial agent for preventing and treating crop root diseases and an application thereof, belonging to the technical field of microbial agents, wherein the microbial agent comprises the following components in parts by weight: 1-5 parts of pseudomonas fluorescens, 2-6 parts of bacillus curvatus, 1-3 parts of bacillus subtilis, 1.5-4.5 parts of lactobacillus plantarum, 4-8 parts of antioxidant and 4-9 parts of stabilizer. In the invention, the microbial agent can prevent and treat crop root diseases, and can prevent and treat pepper bacterial wilt, strawberry root rot, soybean root rot and cucumber fusarium wilt. The microbial agent provided by the invention can be used for preventing and treating crop root diseases, and the prevention and treatment effect is over 85%.
Description
Technical Field
The invention belongs to the technical field of microbial preparations, and particularly relates to a microbial preparation for preventing and treating crop root diseases and an application thereof.
Background
Plant diseases refer to the phenomenon that plants generate a series of pathological changes in form, physiology and biochemistry under the influence of biological or non-biological factors, and the normal growth and development processes are blocked, so that the economic benefit of human beings is influenced. The process of a plant's physiological dysfunction, disruption of tissue architecture under the influence of an insult or adverse environmental conditions of a pathogen, a plant disease is an antagonistic symbiosis of a host plant and a pathogen, the occurrence and prevalence of which are the result of the interaction of the host plant and the pathogen. The occurrence of plant diseases often causes serious loss to national economy and people's life.
The control of the diseases of the roots of crops often depends on chemical control, the use of bactericides causes drug resistance of part of pathogenic bacteria, and the use amount of the bactericides has to be increased, which inevitably causes environmental pollution and pesticide residues of agricultural products.
Disclosure of Invention
In view of the above, the present invention aims to provide a microbial agent for preventing and treating crop root diseases and an application thereof.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a microbial agent for preventing and treating crop root diseases, which comprises the following components in parts by weight:
1-5 parts of pseudomonas fluorescens, 2-6 parts of bacillus curvatus, 1-3 parts of bacillus subtilis, 1.5-4.5 parts of lactobacillus plantarum, 4-8 parts of antioxidant and 4-9 parts of stabilizer.
Preferably, the composition comprises the following components in parts by weight:
2-4 parts of pseudomonas fluorescens, 3-5 parts of bacillus curvatus, 1.5-2.5 parts of bacillus subtilis, 2-4 parts of lactobacillus plantarum, 5-7 parts of antioxidant and 5-8 parts of stabilizer.
Preferably, the effective viable count of the pseudomonas fluorescens is 1 × 1010cfu/g or more.
Preferably, the effective viable count of the Bacillus flexus is 2 × 109cfu/g or more.
Preferably, the effective viable count of the bacillus subtilis is 1.5 × 1010cfu/g or more.
Preferably, the effective viable count of the lactobacillus plantarum is 6 × 108cfu/g or more.
Preferably, the antioxidant comprises one or more of dibutyl hydroxy toluene, tert-butyl hydroquinone and propyl gallate.
Preferably, the stabilizer comprises one or more of malic acid, citric acid, tartaric acid and sorbic acid.
The invention also provides application of the microbial agent in the technical scheme in prevention and treatment of crop root diseases.
Preferably, the crop root diseases comprise pepper bacterial wilt, strawberry root rot, soybean root rot or cucumber fusarium wilt.
The invention provides a microbial agent for preventing and treating crop root diseases and an application thereof, wherein the microbial agent comprises the following components in parts by weight: 1-5 parts of pseudomonas fluorescens, 2-6 parts of bacillus curvatus, 1-3 parts of bacillus subtilis, 1.5-4.5 parts of lactobacillus plantarum, 4-8 parts of antioxidant and 4-9 parts of stabilizer. In the invention, the microbial agent can prevent and treat crop root diseases, and can prevent and treat pepper bacterial wilt, strawberry root rot, soybean root rot and cucumber fusarium wilt. The microbial agent provided by the invention can be used for preventing and treating crop root diseases, and the prevention and treatment effect is over 85%.
Detailed Description
The invention provides a microbial agent for preventing and treating crop root diseases, which comprises the following components in parts by weight: 1-5 parts of pseudomonas fluorescens, 2-6 parts of bacillus curvatus, 1-3 parts of bacillus subtilis, 1.5-4.5 parts of lactobacillus plantarum, 4-8 parts of antioxidant and 4-9 parts of stabilizer.
The microbial agent provided by the invention comprises 1-5 parts of pseudomonas fluorescens, preferably 2-4 parts, and the effective viable count of the pseudomonas fluorescens is preferably 1 × 1010The source of the pseudomonas fluorescens is not particularly limited by the invention, and the pseudomonas fluorescens can be prepared by adopting conventional strains, the preparation method of the pseudomonas fluorescens is not particularly limited by the invention, and the effective viable count of the pseudomonas fluorescens is preferably 1 × 10 by adopting the conventional preparation method10The cfu/g is more than that.
The microbial agent provided by the invention comprises 2-6 parts by weight of Bacillus curvatus, preferably 3-5 parts by weight of Bacillus curvatus, wherein the effective viable count of the Bacillus curvatus is 2 × 109The source of the campylobacter is not particularly limited, the campylobacter can be prepared by adopting conventional strains, the preparation method of the campylobacter is not particularly limited, and the effective viable count of the campylobacter is preferably 2 × 10 by adopting the conventional preparation method9The cfu/g is more than that.
The microbial agent provided by the invention comprises 1-3 parts by weight of bacillus subtilis, preferably 1.5-2.5 parts by weight of bacillus subtilis, wherein the effective viable count of the bacillus subtilis is 1.5 × 1010cfu/g or more. The source of the bacillus subtilis is not specially limited, the conventional strains are adopted, the preparation method of the bacillus subtilis is not specially limited, and the effective viable count of the bacillus subtilis is preferably selected by adopting the conventional preparation method1.5×1010The cfu/g is more than that.
The microbial agent provided by the invention comprises 1.5-4.5 parts by weight of lactobacillus plantarum, preferably 2-4 parts by weight of lactobacillus plantarum, and the effective viable count of the lactobacillus plantarum is 6 × 108The source of the lactobacillus plantarum is not particularly limited by the invention, and the lactobacillus plantarum can be prepared by adopting conventional strains, the preparation method of the lactobacillus plantarum is not particularly limited by the invention, and the effective viable count of the lactobacillus plantarum is preferably 6 × 10 by adopting the conventional preparation method8The cfu/g is more than that.
The microbial agent provided by the invention comprises 4-8 parts by weight of antioxidant, preferably 5-7 parts by weight. In the present invention, the antioxidant preferably comprises one or more of dibutyl hydroxy toluene, tert-butyl hydroquinone and propyl gallate, and the source of the antioxidant is not particularly limited in the present invention, and conventional commercial products can be adopted. In the invention, the antioxidant improves the inoxidizability of the microbial agent, prolongs the service life of the microbial agent and maintains the drug effect of the microbial agent.
The microbial agent provided by the invention comprises 4-9 parts of a stabilizer, preferably 5-8 parts. In the present invention, the stabilizer preferably includes one or more of malic acid, citric acid, tartaric acid and sorbic acid. The source of the stabilizer is not particularly limited in the present invention, and a conventional commercially available product may be used. In the present invention, the stabilizer increases the stabilizing property of the microbial agent.
In the present invention, the method for preparing the microbial agent is not particularly limited, and it is preferable to mix the respective components.
The application method of the microbial agent is not particularly limited, the microbial agent is preferably applied by root irrigation and irrigation, and the application amount is preferably 10-30 g/mu, and more preferably 15-25 g/mu.
The invention also provides application of the microbial agent in the technical scheme in prevention and treatment of crop root diseases. In the present invention, the crop root diseases preferably include pepper bacterial wilt, strawberry root rot, soybean root rot or cucumber fusarium wilt.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Uniformly mixing 1 part of pseudomonas fluorescens, 2 parts of bacillus curvatus, 1 part of bacillus subtilis, 1.5 parts of lactobacillus plantarum, 4 parts of butylated hydroxytoluene and 4 parts of malic acid to obtain the microbial agent, wherein the effective viable count of the pseudomonas fluorescens is 1 × 1010cfu/g, the effective viable count of Bacillus flexus is 2 × 109cfu/g, the effective viable count in the bacillus subtilis is 1.5 × 1010cfu/g, the effective viable count of lactobacillus plantarum is 6 × 108cfu/g。
Example 2
Uniformly mixing 5 parts of pseudomonas fluorescens, 6 parts of bacillus curvatus, 3 parts of bacillus subtilis, 4.5 parts of lactobacillus plantarum, 8 parts of tert-butylhydroquinone and 9 parts of sorbic acid to obtain the microbial agent, wherein the effective viable count of the pseudomonas fluorescens is 1 × 1010cfu/g, the effective viable count of Bacillus flexus is 2 × 109cfu/g, the effective viable count in the bacillus subtilis is 1.5 × 1010cfu/g, the effective viable count of lactobacillus plantarum is 6 × 108cfu/g。
Example 3
Uniformly mixing 5 parts of pseudomonas fluorescens, 6 parts of bacillus curvatus, 3 parts of bacillus subtilis, 4.5 parts of lactobacillus plantarum, 8 parts of propyl gallate and 9 parts of tartaric acid to obtain the microbial agent, wherein the effective viable count of the pseudomonas fluorescens is 1 × 1010cfu/g, the effective viable count of Bacillus flexus is 2 × 109cfu/g, the effective viable count in the bacillus subtilis is 1.5 × 1010cfu/g, the effective viable count of lactobacillus plantarum is 6 × 108cfu/g。
Example 4
The crop variety is pepper, the microbial inoculum prepared in the example 1 is used for root irrigation of pepper at the early stage of the onset of pepper bacterial wilt, the using amount is 15 g/mu, the root irrigation is performed for 1 time at intervals of 4d, the root irrigation is performed for 3 times, after the root irrigation is performed for the last 1 time for 5d, the prevention and control effect is calculated, and no treatment is performed in contrast. The results are shown in Table 1.
TABLE 1 Pepper bacterial wilt control Effect
| Control effect (%) | |
| Example 1 | 85.3 |
| Control | 1.4 |
As can be seen from Table 1, the control effect on the pepper bacterial wilt is greatly improved by using the microbial agent.
Example 5
The crop variety is strawberry, the microbial agent prepared in the example 2 is used for root irrigation of the strawberry at the early stage of the root rot of the strawberry, the using amount is 20 g/mu, the root irrigation is performed for 1 time at intervals of 3d, the root irrigation is performed for 3 times, after the root irrigation is performed for the last 1 time for 5d, the prevention and control effect is calculated, and no treatment is performed in contrast. The results are shown in Table 2.
TABLE 2 prevention and control Effect of strawberry root rot
| Control effect (%) | |
| Example 2 | 87.5 |
| Control | 0.6 |
As can be seen from Table 2, the use of the microbial agent greatly improves the control effect on the root rot of strawberry.
Example 6
The crop variety is soybean, the microbial agent prepared in the example 3 is applied to the soybean in the early stage of the root rot of the soybean, the application amount is 30 g/mu, the application is performed for 1 time at intervals of 6d, the application is performed for 3 times, after the application is performed for 5d for the last 1 time, the control effect is calculated, and no treatment is performed in contrast. The results are shown in Table 3.
TABLE 3 controlling effect on Soybean root rot
| Control effect (%) | |
| Example 3 | 89.4 |
| Control | 0.6 |
As can be seen from Table 3, the control effect on soybean root rot is greatly improved by using the microbial agent.
Example 7
The crop variety is cucumber fusarium wilt, the microbial agent prepared in the example 2 is used for root irrigation of cucumbers at the early stage of the disease onset of the cucumber fusarium wilt, the using amount is 18 g/mu, the roots are irrigated for 3 times at intervals of 4d for 1 time, the prevention and treatment effect is calculated after the roots are irrigated for the last 1 time for 5d, and the control is not processed. The results are shown in Table 4.
TABLE 4 preventive and therapeutic effects on cucumber fusarium wilt
| Control effect (%) | |
| Example 1 | 86.7 |
| Control | 0.7 |
As can be seen from Table 4, the control effect on cucumber fusarium wilt was greatly improved by using the microbial agent.
The embodiments show that the microbial agent provided by the invention can prevent and treat crop root diseases, and the prevention and treatment effect is over 85%.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (4)
1. A microbial agent for preventing and treating crop root diseases is characterized by comprising the following components in parts by weight:
1-5 parts of pseudomonas fluorescens, 2-6 parts of bacillus curvatus, 1-3 parts of bacillus subtilis, 1.5-4.5 parts of lactobacillus plantarum, 4-8 parts of antioxidant and 4-9 parts of stabilizer;
the fluorescent pseudomonadThe effective viable count of the bacteria is 1 × 1010cfu/g or more;
the effective viable count of the curvularia bacillus is 2 × 109cfu/g or more;
the effective viable count of the bacillus subtilis is 1.5 × 1010cfu/g or more;
the effective viable count of the lactobacillus plantarum is 6 × 108cfu/g or more;
the crop root diseases comprise pepper bacterial wilt, strawberry root rot, soybean root rot or cucumber fusarium wilt.
2. The microbial agent according to claim 1, comprising the following components in parts by weight:
2-4 parts of pseudomonas fluorescens, 3-5 parts of bacillus curvatus, 1.5-2.5 parts of bacillus subtilis, 2-4 parts of lactobacillus plantarum, 5-7 parts of antioxidant and 5-8 parts of stabilizer.
3. The microbial inoculant according to claim 1, wherein the antioxidant comprises one or more of dibutylhydroxytoluene, tert-butylhydroquinone and propyl gallate.
4. The microbial inoculant according to claim 1, wherein the stabilizer comprises one or more of malic acid, citric acid, tartaric acid and sorbic acid.
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| CN112094779B (en) * | 2020-09-25 | 2021-05-28 | 安徽希普生物科技有限公司 | Compound microbial preparation containing bacillus subtilis |
| CN112322558A (en) * | 2020-12-08 | 2021-02-05 | 中诚国联（河南）生物科技有限公司 | Compound microbial agent for inhibiting pathogenic bacteria and preparation method and application thereof |
| CN115368179A (en) * | 2022-07-25 | 2022-11-22 | 广西沃瑞德生物科技有限公司 | Organic fertilizer for preventing and treating tomato root rot and preparation method thereof |
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Denomination of invention: A Microbial Agent for Controlling Crop Root Diseases and Its Application Effective date of registration: 20221212 Granted publication date: 20200710 Pledgee: Postal Savings Bank of China Limited Anqiu sub branch Pledgor: WEIFANG LVWEITE BIOLOGICAL ENGINEERING CO.,LTD. Registration number: Y2022980026422 |
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