CN107087640A - A kind of bactericidal composition and its application containing bacillus amyloliquefaciens and Prochloraz - Google Patents
A kind of bactericidal composition and its application containing bacillus amyloliquefaciens and Prochloraz Download PDFInfo
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- CN107087640A CN107087640A CN201710358739.3A CN201710358739A CN107087640A CN 107087640 A CN107087640 A CN 107087640A CN 201710358739 A CN201710358739 A CN 201710358739A CN 107087640 A CN107087640 A CN 107087640A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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Abstract
The invention discloses a kind of bactericidal composition containing bacillus amyloliquefaciens and Prochloraz and its application, said composition is using bacillus amyloliquefaciens and Prochloraz as active component, and its mass ratio is 6:1~1:6.The invention also discloses the preparation method and its application in terms of preventing and treating Strawberry anthracnose and gray mold of the agricultural formulation wettable powder of the bactericidal composition and suspending agent.Preventing and treating of the bactericidal composition of the present invention to Strawberry anthracnose and gray mold compared with single dose has obvious synergistic effect, is remarkably improved prevention effect；Strawberry anthracnose bacterium and ash arrhizus bacteria are reduced to Prochloraz and the potential resistance risk of bacillus；Chemical pesticide usage amount is reduced, drug cost is reduced, the residue problem of environmental pollution and agricultural product is effectively improved, to people and animals, beneficial organism, Environmental security, so as to be widely used in the preventing and treating of Strawberry anthracnose and gray mold.
Description
Technical field
The invention belongs to pesticide field, and in particular to one kind contains bacillus amyloliquefaciens（Bacillus amyloliquefaciens）Composition with Prochloraz and its application in terms of preventing and treating Strawberry anthracnose and grey mould fruit rot of strawberry.
Background technology
Strawberry is beautiful in colour, with rich flavor, nutritious, high financial profit, be high-grade fruit in current fruit market
One of, cultivated extensively in multiple provinces and cities of China, and cultivated area is in the trend risen year by year.For improve strawberry product yield and
Quality, extends the market supply time, current China the greater part is used using greenhouse and heliogreenhouse as main Protectorate cultivation mould
Formula is planted.Under this cultivation mode, because environment is relatively closed, ventilating air permeability is poor, and temperature, humidity are suitable, and changes
The excessive administration of fertilizer is learned, strawberry easily produces various pest and disease damages.
Strawberry anthracnose is one of the Major Diseases in China strawberry producing region, disease main harm stolon, petiole, the leaf
Piece, stipule, flower, fruit and root can also be infected, and local scab and complete stool can be caused to wilt.The disease occurs the most general in nursery stage
Time, in numerous seedling stage and the initial stage of field planting, the incidence of disease is sometime up to 90%, or even the crushing dead seedling of large area also happens occasionally, and makes
Into serious economic loss.In recent years, with the expansion of strawberry cultivating area in the whole country, facility is closed and hot and humid ring
It is increasingly serious that border etc. causes Strawberry anthracnose to occur, and causes the strawberry underproduction 25%~30%, has a strong impact on the yield and product of strawberry
Matter.
Grey mould fruit rot of strawberry is in strawberry production and adopts a kind of important disease of rear strawberry storage phase, is especially bloomed in strawberry
The environmental condition that fruiting period runs into low temperature and high relative humidity is easily caused generation and the prevalence of disease, causes rotting for flower and fruit, to grass
Certain kind of berries yield, qualitative effects are very big, lose more serious.The pathogen of grey mould fruit rot of strawberry is Botrytis cinerea, in ten big pathogenics
Second is listed in fungi.
Chemical prevention is still the Main Means for preventing and treating Strawberry anthracnose and gray mold at present, for a long time due to bactericide
Many frequencys, the use of multiple dose so that the increase of the pathogen resistance to the action of a drug, prevention effect decline, the agricultural chemicals of environmental pollution and fruit
A series of problems, such as residual, is more protruded, therefore seeks a kind of prevention and controls of highly effective and safe and be particularly important, and strawberry is produced
The sustainable development of industry has great importance.
Bacillus amyloliquefaciens（Bacillus amyloliquefaciens）Belong to Grain-positive bacillus, be it is a kind of with
Bacillus subtilis affinity very high bacterium, it is widely distributed in nature.Can be with bacillus amyloliquefaciens growth course
A series of active metabolites for having and widely suppressing fungi and bacterium are produced, there is wide answer in terms of biological control
With prospect, progressively turn into the microorganism with biological pesticide potentiality to be exploited.Bacillus amyloliquefaciens WH1G, antimicrobial spectrum is wider,
There is very strong inhibitory action to a variety of disease funguses and pathogenetic bacteria, the strain growth speed is fast, accommodative ability of environment is strong, easily
Colonized in plant surface, and form dominant population；Host is induced to produce disease resistance；Secrete a variety of antibacterial materials；To strawberry charcoal
The prevention effect of subcutaneous ulcer germ and Botrytis cinerea germ is preferable.The bacterial strain has natural resistance to Prochloraz, is in Prochloraz content
In 5000ppm NA culture mediums can normal growth, the bacterial strain and be preserved within 04 15th, 2015 Chinese microorganism strain guarantor
Administration committee's common micro-organisms center is hidden, preserving number is CGMCC No.10722.The Strain Designation is WHIG, Classification And Nomenclature：
Bacillus amyloliquefaciens（Bacillus amyloliquefaciens）.
Prochloraz is a kind of wide-spectrum bactericide, is had to various crop disease as caused by sac fungus and Fungi Imperfecti obvious
Preventive effect, can also be mixed with most of bactericide, Insecticides (tech) ＆ Herbicides (tech), there is preferable prevention effect.To field crop, water
Multiple diseases on fruit vegetable, turf and ornamental plant have treatment and eradicant action.Prochloraz is in America and Europe, the Middle East, China, day
The multiple countries and regions in the whole world such as this promote the use of a large area for many years, are one of main antistaling agent and bactericide of melon and fruit class.
Due to repeatedly, continuously being used in China in recent years so that disease fungus constantly increases the drug-resistant populations of Prochloraz.Exist at present
The Prochloraz product majority applied in China's Diseases of Strawberry preventing and treating is single dose, and only a small number of built agents are also and other chemical drugs
Agent is compounded, and acts on limited for delaying and administering the resistance to the action of a drug of Prochloraz.So far Prochloraz and biocontrol microorganisms are not found also
Complex agent type is used for the preventing and treating of Diseases of Strawberry, and the present invention is careless in preventing and treating using the composition of bacillus amyloliquefaciens and Prochloraz
Application in certain kind of berries anthracnose and gray mold does not disclose report also.
The content of the invention
It is an object of the invention to：During for currently with existing bactericidal agent for preventing and treating Strawberry anthracnose and grey mould fruit rot of strawberry
A series of problems of appearance can improve drug effect, extension medicament use time there is provided one kind, reduce cost, synergistic function shows
Write, there is preferable preventive and therapeutic effect, and environment-friendly, safe organic bactericide composition to Strawberry anthracnose and gray mold.The group
Compound greatly reduces the use of chemical bactericide using bacillus amyloliquefaciens and Prochloraz as main active substances, not only
Amount, reduces potential drug-fast risk of the existing pathogen to Prochloraz, moreover it is possible to play bacillus amyloliquefaciens WH1G effective
The effect of Strawberry anthracnose and gray mold is controlled, the slow shortcoming of bacillus amyloliquefaciens fungistatic effect is compensate for, combines biological and ecological methods to prevent plant disease, pests, and erosion
Bacterium and the advantage of chemical bactericide.This result of study shows the combination of bacillus amyloliquefaciens WHIG and Prochloraz to strawberry anthrax
The preventive effect of disease and gray mold has obvious synergistic effect.
Bactericidal composition of the present invention can be processed into the formulation of suitable agricultural use with known method, including can
WP, suspending agent, aqueous emulsion etc., the invention provides the preparation side of the wettable powder of the bactericide and aqueous suspension agent
Method.
Auxiliary agent in composition of the present invention includes wetting agent, dispersant, bleeding agent, antifreezing agent, thickener, synergy
One or more in each analog assistant such as agent, defoamer, specific composition and consumption are determined according to recipe requirements by simple experiment.
The purpose of the present invention can be reached by following measures:
A kind of WHIG containing bacillus amyloliquefaciens and Prochloraz composition, said composition is with bacillus amyloliquefaciens WHIG and miaow
Fresh amine is active component, and its mass ratio is 500:1~1：500；The mass ratio of bacillus amyloliquefaciens WHIG and Prochloraz is preferably
100：1~1:100, further it is optimized for 20:1~1:20, most preferably 6:1~1:6.Solution starch gemma bar of the present invention
Bacterium live spore count be 100,000,000/gram ~ 10,000,000,000/gram, most preferably 1,000,000,000/gram ~ 10,000,000,000/gram.
Female powder will be first made in bacillus amyloliquefaciens in bactericidal composition of the present invention, and method is as follows：First will
Shaken cultivation (180 r/min, 28 DEG C) 48 h, 80 DEG C of water bath processings 15 min, 8000 r/ in WH1G access NA culture mediums
Min centrifuges 15 min, goes supernatant to obtain bacterial precipitation, adds the MgSO of 10 mL filtration sterilizations4Solution (0.l mol/L)
With 1mL sterile glycerols, vibration, which is mixed, adds l mL concentration for 1.5% (w/v) sodium alginate soln after Uniform, then by above-mentioned mixing
Liquid is sufficiently mixed with 50g talcum powder and 4g Ca-lignosulphate, room temperature preservation under aseptic condition.
The survival ability of bacillus amyloliquefaciens female powder is determined every 30d in the composition, and wettable powder is determined respectively
The survival ability of biocontrol microorganisms in agent, when method is each determines, weighs 0.5 gram of wettable powder, is placed in 10mL sterilized waters respectively
In, suspension is prepared, after doubling dilution, is respectively coated on NA flat boards, 28 DEG C of culture 24h count growth bacterium colony, calculated
The CFU of bacterium in Unit Weight wettable powder.As a result show, the survival ability of bacterium is stronger in biocontrol agent,
The amount of survival that 120d bacteriums are preserved under room temperature condition remains to reach 1010Cfu/g, 150d can reach 108Cfu/g, over time
Extension, bacterial number constantly declines, is only capable of reaching 10 to 180d7cfu/g。
Described bactericidal composition adds insecticides adjuvant and filler into active component bacillus amyloliquefaciens and Prochloraz
After can be with to obtain wettable powder and suspending agent, wherein active ingredient accounts for the 20% ~ 60% of bactericidal composition gross weight, and auxiliary agent, which is accounted for, to kill
The 10% ~ 30% of bacteria composition gross weight, remaining is filler.Prochloraz can use active compound, and bacillus amyloliquefaciens use former powder,
Auxiliary agent can be from one kind or several in each analog assistants such as wetting agent, dispersant, bleeding agent, antifreezing agent, thickener, synergist
Kind.
When the formulation of the bactericidal composition is wettable powder, it is made up of the component of following weight/mass percentage compositions：
Bacillus amyloliquefaciens female powder（10000000000 work spores/gram）5%~50%,
Prochloraz 10% ~ 40%,
Carrier 30 as one kind % ~ 65%,
Dispersant 3% ~ 8%,
Wetting agent 2% ~ 5%,
Bleeding agent 3% ~ 5%
It is characterized in that the carrier is preferably white carbon, kaolin, the dispersant is preferably sodium lignin sulfonate, the wetting
Agent is preferably DBSA, and the bleeding agent is preferably JFC.
When the formulation of the bactericidal composition is aqueous suspension agent, it is made up of the component of following weight/mass percentage compositions：
Bacillus amyloliquefaciens female powder（10000000000 work spores/gram）5%~50%,
Prochloraz 5% ~ 45%,
Dispersant 2% ~ 8%,
Wetting agent 2% ~ 4%,
Thickener 0.2% ~ 2%
Synergist 2% ~ 5%
Antifreezing agent 2% ~ 4%
Defoamer 1% ~ 3%
Surplus adds water to 100%,
It is characterized in that the dispersant is preferably calcium lignosulfonate, wetting agent is preferably lauryl sodium sulfate, and thickener is excellent
Elect xanthans as, selected antifreezing agent is preferably ethylene glycol；Defoamer is preferably organic silicon defoamer, and water is preferably running water.
The bactericidal composition of the present invention is used to prevent and treat Strawberry anthracnose and gray mold, is applied when disease tentatively occurs
Medicine, continuous administration 3 times, interval is 10d, is advisable with the uniform dispenser of spray-on process.
The composition of the present invention reduces Strawberry anthracnose bacterium and ash arrhizus bacteria to Prochloraz and bacillus amyloliquefaciens
Potential resistance risk,, can be with therebetween with obvious synergistic effect after bacillus amyloliquefaciens are combined with Prochloraz
The prevention effect to Strawberry anthracnose and gray mold is significantly improved, while chemical pesticide usage amount is reduced, so that the combination
Thing can be effectively applied to the preventing and treating of Strawberry anthracnose and gray mold.
Embodiment
The present invention is further explained with reference to specific embodiment, but the essence of the present invention is not limited to following embodiments.
Embodiment 1：The preparation of 35% wettable powder（Each group is divided into mass parts）
[bacillus amyloliquefaciens WH1G（10000000000 work spores/gram） 5
Prochloraz 30
Lauryl sodium sulfate 2
Sodium lignin sulfonate 4
Penetrating agent JFC 4
White carbon supply is to 100（Adjust pH value 5 ~ 7）
Embodiment 2：The preparation of 40% wettable powder（Each group is divided into mass parts）
Bacillus amyloliquefaciens WH1G（10000000000 work spores/gram） 20
Prochloraz 20
Lauryl sodium sulfate 2
Sodium lignin sulfonate 4
Penetrating agent JFC 4
White carbon supply is to 100（Adjust pH value 5 ~ 7）
Embodiment 3：It is prepared by 45% wettable powder（Each group is divided into mass parts）
Bacillus amyloliquefaciens WH1G（10000000000 work spores/gram） 15
Prochloraz 30
Lauryl sodium sulfate 2
Sodium lignin sulfonate 4.5
Penetrating agent JFC 4.5
White carbon 30
Kaolin complements to 100（Adjust pH value 5 ~ 7）
Embodiment 4：It is prepared by 55% wettable powder（Each group is divided into mass parts）
Bacillus amyloliquefaciens WH1G（10000000000 work spores/gram） 44
Prochloraz 11
Lauryl sodium sulfate 3
Sodium lignin sulfonate 5
Penetrating agent JFC 5
White carbon 20
Kaolin complements to 100（Adjust pH value 5 ~ 7）
Embodiment 5：It is prepared by 60% wettable powder（Each group is divided into mass parts）
Bacillus amyloliquefaciens WH1G（10000000000 work spores/gram） 40
Prochloraz 20
Lauryl sodium sulfate 3
Sodium lignin sulfonate 5
Penetrating agent JFC 5
White carbon 20
Kaolin complements to 100（Adjust pH value 5 ~ 7）
According to the formula of embodiment 1,2,3,4,5, active ingredient, auxiliary agent, filler are added together, after air-flow crushing, in cone
Stirring 30 minutes in shape mixer, you can the sterilization wettable powder of bacillus amyloliquefaciens and Prochloraz is made.
Embodiment 6:The preparation of 25% suspending agent（Each group is divided into mass parts）
Bacillus amyloliquefaciens WH1G（10000000000 work spores/gram） 10
Prochloraz 15
Calcium lignosulfonate 5
Lauryl sodium sulfate 2
Organosilicon 2
Xanthans 0.3
Ethylene glycol 2
Running water complements to 100
Embodiment 7:The preparation of 49% suspending agent（Each group is divided into mass parts）
Bacillus amyloliquefaciens WH1G（10000000000 work spores/gram） 42
Prochloraz 7
Calcium lignosulfonate 6
Lauryl sodium sulfate 3
Organosilicon 2
Xanthans 0.3
Ethylene glycol 3
Running water complements to 100
Embodiment 8:The preparation of 50% suspending agent（Each group is divided into mass parts）
Bacillus amyloliquefaciens WH1G（10000000000 work spores/gram） 10
Prochloraz 40
Calcium lignosulfonate 7
Lauryl sodium sulfate 4
Organosilicon 2
Xanthans 0.2
Ethylene glycol 3.5
Running water complements to 100
According to the formula of embodiment 6,7,8, each groups such as active ingredient, dispersant, wetting agent are distributed into what is stirred with high shear
In reactor, it is pulverized and mixed, the material for stirring crushing is passed through in sand mill after mixture is uniform, logical cooling circulating water enters
Ultramicro grinding is sanded in row, and terminal particle diameter is less than 3 microns.It can be prepared by the sterilization aqueous suspension of bacillus amyloliquefaciens and Prochloraz
Agent.
The bacillus amyloliquefaciens and the bactericidal composition of Prochloraz that embodiment 9 is configured with embodiment 1- embodiments 8 are prevented
Control the field efficacy of Strawberry anthracnose and grey mould fruit rot of strawberry.
Trial crops of the present invention are strawberry, and kind is：Beauty；The solution starch for setting embodiment 1- embodiments 8 to be configured altogether
The bactericidal composition of bacillus and Prochloraz, 10,000,000,000 CFU/g bacillus amyloliquefaciens female powders, 50% Prochloraz wettable powder
With blank control totally 11 processing, each 4 repetitions of processing, the ㎡ of plot area 20 starts to spray for the first time in disease early period of origination
Medicine, every mu is watered 50 kilograms of even sprayings, and continuous to spray 3 times, interval is 10d, and 7d investigates each processing after last time is prevented and treated
The disease index of Strawberry anthracnose and gray mold, and relative prevention effect is calculated with the disease index of check plot, investigation result is such as
Table 1.
Table 1 solves preventive effect of the shallow lake powder bacillus ﹒ Prochlorazs to the field plot trial of Strawberry anthracnose and gray mold（%）
Note：a:Theoretical preventive effect %=（Bacillus single dose preventive effect × proportion+Prochloraz preventive effect × proportion in embodiment）
×100
b:Synergistic effect %=（Embodiment prevention effect-theory preventive effect）/ theory preventive effect × 100
Dispenser is begun in Strawberry anthracnose and gray mold first meeting scab as can be seen from Table 1, is then applied 1 time at interval of 10d
Medicine, after continuously spraying 3 times, 8 example of formulations of the present composition are equal to the preventive effect of Strawberry anthracnose and grey mould fruit rot of strawberry
More than 80%, hence it is evident that higher than comparison medicament, preventive effect is notable.Embodiment 1.2.3.5.8 is to the preventive effect of Strawberry anthracnose 85%
More than, embodiment 1-8 is to the preventive effect of grey mould fruit rot of strawberry more than 80%, and particularly embodiment 8 is to Strawberry anthracnose and strawberry ash
The preventive effect of mildew is up to 89.64% and 86.72%, and preventive effect is extremely notable；The present composition is compared with single dose, there is synergistic effect,
To the enhancing proportions of Strawberry anthracnose between 10%-20%, the particularly synergistic effect of embodiment 7 is up to 19.14%, to strawberry ash
The enhancing proportions of mildew are up to 27.92% and 25.67% respectively in 16%-28%, particularly embodiment 5 and the synergistic effect of embodiment 7,
Synergistic effect substantially, is remarkably improved prevention effect；The present composition grows safety to strawberry, and field does not find any bad
Reaction, so said composition can be widely used in preventing and treating Strawberry anthracnose and gray mold in production.
Claims (7)
1. a kind of bactericidal composition containing bacillus amyloliquefaciens and Prochloraz, it is characterised in that said composition is to solve starch gemma
Bacillus and Prochloraz are active component, and its mass ratio is 20:1~1:20, optimum ratio is 6:1~1:6.
2. the composition according to claim 1 containing bacillus amyloliquefaciens and Prochloraz, it is characterised in that：Described solution
The entitled WH1G of bacillus amyloliquefacienses, preservation number is CGMCC NO. 10722.
3. the composition containing bacillus amyloliquefaciens and Prochloraz according to claim 1,2, it is characterised in that described
In bacillus amyloliquefaciens spore count living be 1,000,000,000/gram ~ 10,000,000,000/gram.
4. the composition containing bacillus amyloliquefaciens and Prochloraz according to claim 1,2,3, it is characterised in that to work
Property composition bacillus amyloliquefaciens and Prochloraz in add after auxiliary agent and filler, you can with must be adapted to agriculturally use agricultural dose
Type, such as wettable powder and aqueous suspension agent.
5. the auxiliary agent used can be each from wetting agent, dispersant, bleeding agent, antifreezing agent, thickener, synergist, defoamer etc.
One or more in analog assistant;The filler used can be precipitated calcium carbonate, white carbon, kaolin, attapulgite, organic bentonite
One or more in profit soil.
6. the composition according to claim 4 containing bacillus amyloliquefaciens and Prochloraz, it is characterised in that wherein effective
Composition accounts for the 20% ~ 60% of bactericidal composition gross weight, and auxiliary agent accounts for the 10% ~ 30% of bactericidal composition gross weight, and remaining is filler.
7. the composition according to claim 5 containing bacillus amyloliquefaciens and Prochloraz is in preventing and treating Strawberry anthracnose and grass
Application in terms of certain kind of berries gray mold.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107912447A (en) * | 2017-11-23 | 2018-04-17 | 贵州省烟草科学研究院 | A kind of microbicide compositions containing bacillus amyloliquefaciens, preparation method and the usage |
| CN110558332A (en) * | 2019-10-22 | 2019-12-13 | 天津市植物保护研究所 | Synergistic bactericidal composition containing bacillus amyloliquefaciens B1126 and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107912447A (en) * | 2017-11-23 | 2018-04-17 | 贵州省烟草科学研究院 | A kind of microbicide compositions containing bacillus amyloliquefaciens, preparation method and the usage |
| CN110558332A (en) * | 2019-10-22 | 2019-12-13 | 天津市植物保护研究所 | Synergistic bactericidal composition containing bacillus amyloliquefaciens B1126 and application thereof |
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| CN107087640B (en) | 2020-07-21 |
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