CN107079928A - A kind of bactericidal composition containing bacillus amyloliquefaciens and Boscalid - Google Patents
A kind of bactericidal composition containing bacillus amyloliquefaciens and Boscalid Download PDFInfo
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- CN107079928A CN107079928A CN201710358479.XA CN201710358479A CN107079928A CN 107079928 A CN107079928 A CN 107079928A CN 201710358479 A CN201710358479 A CN 201710358479A CN 107079928 A CN107079928 A CN 107079928A
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- boscalid
- bacillus amyloliquefaciens
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- strawberry
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
Abstract
The invention discloses a kind of bactericidal composition containing bacillus amyloliquefaciens and Boscalid and its application, said composition is using bacillus amyloliquefaciens and Boscalid as active component, and its mass ratio is 4:1~1:4.The invention also discloses the preparation method and its application in terms of grey mould fruit rot of strawberry is prevented and treated of the agricultural formulation wettable powder of the bactericidal composition and suspending agent.Preventing and treating of the bactericidal composition of the present invention to grey mould fruit rot of strawberry compared with single dose has obvious synergistic effect, is remarkably improved prevention effect；Botrytis cinerea germ is reduced to Boscalid and the potential resistance risk of bacillus；Chemical pesticide usage amount is reduced, drug cost is reduced, the residue problem of environmental pollution and agricultural product is effectively improved, to people and animals, beneficial organism, Environmental security, so as to be widely used in the preventing and treating of grey mould fruit rot of strawberry.
Description
Technical field
The invention belongs to pesticide field, and in particular to one kind contains bacillus amyloliquefaciens（Bacillus amyloliquefaciens）Composition with Boscalid and its application in terms of grey mould fruit rot of strawberry is prevented and treated.
Background technology
The scarlet shape of strawberry fruit is beautiful, and soft and succulency, aromatic flavour, sweet tea acid is tasty, nutritious, is in current fruit market
One of high-grade fruit, be well received by consumers, wide market.In recent years, our province various regions cultivated area expands rapidly,
Cultivated extensively in multiple provinces and cities of China, and cultivated area is in the trend risen year by year.To improve the yield and quality of strawberry product,
Extend the market supply time, current China the greater part is used to be carried out using greenhouse and heliogreenhouse as main Protectorate cultivation pattern
Plantation.Under this cultivation mode, because environment is relatively closed, ventilating air permeability is poor, and temperature, humidity are suitable, and chemical fertilizer
Excessive administration, strawberry easily produces various pest and disease damages.
Grey mould fruit rot of strawberry is one of Major Diseases of strawberry, main harm floral organ and fruit, causes floral organ and fruit rot,
This disease can be propagated rapidly, be the main cause for causing strawberry decayed fruit, typically make strawberry underproduction 10%-30%, grass can be caused when serious
The certain kind of berries underproduction more than 50%, has a strong impact on the yield and quality of strawberry.Current chemical agent is still the main of preventing and treating grey mould fruit rot of strawberry
Measure, due to many frequencys of chemical agent, the use of multiple dose, cause resistance to the action of a drug increase, prevention effect to decline, environmental pollution and
A series of problems, such as residues of pesticides of fruit, more protrudes, therefore seeks a kind of prevention and controls of highly effective and safe and seem particularly heavy
Will, the sustainable development to Strawberry industry has great importance.
Bacillus amyloliquefaciens（Bacillus amyloliquefaciens）Belong to Grain-positive bacillus, be it is a kind of with
Bacillus subtilis affinity very high bacterium, it is widely distributed in nature.Can be with bacillus amyloliquefaciens growth course
A series of active metabolites for having and widely suppressing fungi and bacterium are produced, there is wide answer in terms of biological control
With prospect, progressively turn into the microorganism with biological pesticide potentiality to be exploited.Bacillus amyloliquefaciens WH1G, antimicrobial spectrum is wider,
There is very strong inhibitory action to a variety of disease funguses and pathogenetic bacteria, the strain growth speed is fast, accommodative ability of environment is strong, easily
Colonized in plant surface, and form dominant population；Host is induced to produce disease resistance；Secrete a variety of antibacterial materials；To many plantations
Thing disease has preferable preventive effect.The bacterial strain has natural resistance to Boscalid, is trained in Boscalid content for 5000ppm NA
Energy normal growth in base is supported, the bacterial strain was preserved in general in China Committee for Culture Collection of Microorganisms with 04 15th, 2015
Logical microorganism center, preserving number is CGMCC No.10722.The Strain Designation is WHIG, Classification And Nomenclature：Bacillus amyloliquefaciens
（Bacillus amyloliquefaciens）.Preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3.
Boscalid belongs to nicotinamide new type bactericide, mainly hinders three by suppressing mitochondrial succinate acid esters dehydrogenase
Carboxylic acid recycle, lacks amino acid and sugar, energy is reduced, the division and growth of interference cell.The medicament can be used for control strawberry ash
Mildew, and with the medicament no interactions resistance to the action of a drug such as carbendazim, Sukeling.The pyridine acyl bacterium applied at present in China's Diseases of Strawberry preventing and treating
Amine product majority is single dose, and only a small number of built agents are also to be compounded with other chemical agents, for delaying and administering pyridine acyl bacterium
The resistance to the action of a drug effect of amine is limited.So far not finding the complex agent type of Boscalid and biocontrol microorganisms also is used for the preventing and treating of Diseases of Strawberry, and originally
Invention does not disclose report also using application of the composition of bacillus amyloliquefaciens and Boscalid on preventing and treating grey mould fruit rot of strawberry.
The content of the invention
It is an object of the invention to：What is occurred during for currently with existing bactericidal agent for preventing and treating grey mould fruit rot of strawberry is a series of
Problem there is provided it is a kind of efficiently, low toxicity, medicament use time can be extended, cost, obvious synergy are reduced, to strawberry ash
Mildew has preferable preventive and therapeutic effect, and environment-friendly, safe agricultural bactericidal composition.Said composition is with bacillus amyloliquefaciens
It is main active substances with Boscalid, not only greatly reduces the usage amount of chemical bactericide, reduce existing pathogen
To the potential drug-fast risk of Boscalid, moreover it is possible to play the work that bacillus amyloliquefaciens WH1G effectively controls grey mould fruit rot of strawberry
With compensate for the slow shortcoming of bacillus amyloliquefaciens fungistatic effect, combine the advantage of biocontrol microorganisms and chemical bactericide.This research
As a result show that preventing and treating of the combination of bacillus amyloliquefaciens WHIG and Boscalid to grey mould fruit rot of strawberry has obvious synergistic effect.
Bactericidal composition of the present invention can be processed into the formulation of suitable agricultural use with known method, including wettable
Property pulvis, suspending agent, aqueous emulsion etc., the invention provides the preparation method of the wettable powder of the bactericide and aqueous suspension agent.
Auxiliary agent in composition of the present invention includes wetting agent, dispersant, bleeding agent, antifreezing agent, thickener, synergy
One or more in each analog assistant such as agent, defoamer, specific composition and consumption are determined according to recipe requirements by simple experiment.
The purpose of the present invention can be reached by following measures:
A kind of WHIG containing bacillus amyloliquefaciens and Boscalid composition, said composition with bacillus amyloliquefaciens WHIG and
Boscalid is active component, and its mass ratio is 100：1~1:100, it is optimized for 10:1~1:10, most preferably 4:1~1:4.This hair
Bright used bacillus amyloliquefaciens live spore count be 100,000,000/gram ~ 10,000,000,000/gram, most preferably 1,000,000,000/gram ~ 10,000,000,000/gram.
Female powder will be first made in bacillus amyloliquefaciens in bactericidal composition of the present invention, and method is as follows：First will
Shaken cultivation (180 r/min, 28 DEG C) 48 h, 80 DEG C of water bath processings 15 min, 8000 r/ in WH1G access NA culture mediums
Min centrifuges 15 min, goes supernatant to obtain bacterial precipitation, adds the MgSO of 10 mL filtration sterilizations4Solution (0.l mol/L)
With 1mL sterile glycerols, vibration, which is mixed, adds l mL concentration for 1.5% (w/v) sodium alginate soln after Uniform, then by above-mentioned mixing
Liquid is sufficiently mixed with 50g talcum powder and 4g Ca-lignosulphate, room temperature preservation under aseptic condition.
The survival ability of bacillus amyloliquefaciens female powder is determined every 30d in the composition, and wettable powder is determined respectively
The survival ability of biocontrol microorganisms in agent, when method is each determines, weighs 0.5 gram of wettable powder, is placed in 10mL sterilized waters respectively
In, suspension is prepared, after doubling dilution, is respectively coated on NA flat boards, 28 DEG C of culture 24h count growth bacterium colony, calculated
The CFU of bacterium in Unit Weight wettable powder.As a result show, the survival ability of bacterium is stronger in biocontrol agent,
The amount of survival that 120d bacteriums are preserved under room temperature condition remains to reach 1010Cfu/g, 150d can reach 108Cfu/g, over time
Extension, bacterial number constantly declines, is only capable of reaching 10 to 180d7cfu/g。
Described bactericidal composition adds insecticides adjuvant and filler into active component bacillus amyloliquefaciens and Boscalid
After can be with to obtain wettable powder and suspending agent, wherein active ingredient accounts for the 20% ~ 60% of bactericidal composition gross weight, and auxiliary agent accounts for sterilization
The 10% ~ 30% of composition total weight, remaining is filler.Boscalid can use active compound, and bacillus amyloliquefaciens are helped using former powder
Agent can be from the one or more in each analog assistants such as wetting agent, dispersant, bleeding agent, antifreezing agent, thickener, synergist.
When the formulation of the bactericidal composition is wettable powder, it is made up of the component of following weight/mass percentage compositions：
Bacillus amyloliquefaciens female powder（10000000000 work spores/gram） 5%~50%,
Boscalid 5% ~ 40%,
Carrier 30 as one kind % ~ 65%,
Dispersant 3% ~ 8%,
Wetting agent 2% ~ 5%,
Bleeding agent 3% ~ 5%
It is characterized in that the carrier is preferably white carbon, kaolin, the dispersant is preferably sodium lignin sulfonate, the wetting
Agent is preferably DBSA, and the bleeding agent is preferably JFC.
When the formulation of the bactericidal composition is aqueous suspension agent, it is made up of the component of following weight/mass percentage compositions：
Bacillus amyloliquefaciens female powder（10000000000 work spores/gram） 5%~50%,
Boscalid 5% ~ 45%,
Dispersant 2% ~ 8%,
Wetting agent 2% ~ 4%,
Thickener 0.1% ~ 2%
Synergist 2% ~ 5%
Antifreezing agent 2% ~ 4%
Defoamer 1% ~ 3%
Surplus adds water to 100%,
It is characterized in that the dispersant is preferably calcium lignosulfonate, wetting agent is preferably lauryl sodium sulfate, and thickener is excellent
Elect xanthans as, selected antifreezing agent is preferably ethylene glycol；Defoamer is preferably organic silicon defoamer, and water is preferably running water.
The bactericidal composition of the present invention is used to prevent and treat Strawberry anthracnose, powdery mildew and gray mold, when disease tentatively occurs
Progress dispenser, continuous administration 3 times, interval is 10d, is advisable with the uniform dispenser of spray-on process.
The composition of the present invention reduces Botrytis cinerea germ to Boscalid and the potential anti-medicine of bacillus amyloliquefaciens
Property risk, after bacillus amyloliquefaciens are combined with Boscalid, therebetween with obvious synergistic effect, can significantly be carried
The high prevention effect to grey mould fruit rot of strawberry, while chemical pesticide usage amount is reduced, so that said composition can be widely applied
In the preventing and treating of plant ash mildew.
Embodiment
The present invention is further explained with reference to specific embodiment, but the essence of the present invention is not limited to following embodiments.
Embodiment 1：The preparation of 35% wettable powder（Each group is divided into mass parts）
Bacillus amyloliquefaciens WH1G（10000000000 work spores/gram） 7
Boscalid 28
Lauryl sodium sulfate 2
Sodium lignin sulfonate 4
Penetrating agent JFC 4
White carbon supply is to 100（Adjust pH value 5 ~ 7）
Embodiment 2：The preparation of 40% wettable powder（Each group is divided into mass parts）
Bacillus amyloliquefaciens WH1G（10000000000 work spores/gram） 30
Boscalid 10
Lauryl sodium sulfate 2
Sodium lignin sulfonate 4.5
Penetrating agent JFC 4.5
White carbon supply is to 100（Adjust pH value 5 ~ 7）
Embodiment 3：It is prepared by 42% wettable powder（Each group is divided into mass parts）
Bacillus amyloliquefaciens WH1G（10000000000 work spores/gram） 28
Boscalid 14
Lauryl sodium sulfate 2
Sodium lignin sulfonate 4.5
Penetrating agent JFC 4.5
White carbon 30
Kaolin complements to 100（Adjust pH value 5 ~ 7）
Embodiment 4：It is prepared by 50% wettable powder（Each group is divided into mass parts）
Bacillus amyloliquefaciens WH1G（10000000000 work spores/gram） 25
Boscalid 25
Lauryl sodium sulfate 3
Sodium lignin sulfonate 5
Penetrating agent JFC 5
White carbon 20
Kaolin complements to 100（Adjust pH value 5 ~ 7）
According to the formula of embodiment 1,2,3,4, active ingredient, auxiliary agent, filler are added together, after air-flow crushing, in taper
Stirring 30 minutes in mixer, you can the sterilization wettable powder of bacillus amyloliquefaciens and Boscalid is made.
Embodiment 5:The preparation of 30% suspending agent（Each group is divided into mass parts）
Bacillus amyloliquefaciens WH1G（10000000000 work spores/gram） 10
Boscalid 20
Calcium lignosulfonate 5
Lauryl sodium sulfate 2
Organosilicon 2
Xanthans 0.3
Ethylene glycol 2
Running water complements to 100
Embodiment 6:The preparation of 55% suspending agent（Each group is divided into mass parts）
Bacillus amyloliquefaciens WH1G（10000000000 work spores/gram） 44
Boscalid 11
Calcium lignosulfonate 5
Lauryl sodium sulfate 2
Organosilicon 2
Xanthans 0.2
Ethylene glycol 3.5
Running water complements to 100
Embodiment 7:The preparation of 60% suspending agent（Each group is divided into mass parts）
Bacillus amyloliquefaciens WH1G（10000000000 work spores/gram） 15
Boscalid 45
Calcium lignosulfonate 7
Lauryl sodium sulfate 4
Organosilicon 2
Xanthans 0.2
Ethylene glycol 3.5
Running water complements to 100
According to the formula of embodiment 5,6,7, each groups such as active ingredient, dispersant, wetting agent are distributed into what is stirred with high shear
In reactor, it is pulverized and mixed, the material for stirring crushing is passed through in sand mill after mixture is uniform, logical cooling circulating water enters
Ultramicro grinding is sanded in row, and terminal particle diameter is less than 3 microns.It can be prepared by the sterilization aqueous suspension of bacillus amyloliquefaciens and Boscalid
Agent.
Bacillus amyloliquefaciens and the bactericidal composition of Boscalid that embodiment 8 is configured with embodiment 1- embodiments 7
Prevent and treat the field efficacy of grey mould fruit rot of strawberry.
Trial crops of the present invention are strawberry, and kind is：Beauty；The solution starch for setting embodiment 1- embodiments 7 to be configured altogether
The bactericidal composition of bacillus and Boscalid, 10,000,000,000 CFU/g bacillus amyloliquefaciens female powders, 50% Boscalid moisture dissipate
Granula and blank control totally 10 processing, each 4 repetitions of processing, the ㎡ of plot area 20 start first in disease early period of origination
Secondary spray, every mu is watered 50 kilograms of even sprayings, and continuous to spray 2 times, interval is 10d, and 10d investigation is each after last time is prevented and treated
The disease index of grey mould fruit rot of strawberry is handled, and relative prevention effect, investigation result such as table 1 are calculated with the disease index of check plot.
Table 1 solves preventive effect of the shallow lake powder bacillus ﹒ Boscalids to the field plot trial of grey mould fruit rot of strawberry（%）
Note：a:Theoretical preventive effect %=（Bacillus single dose preventive effect × proportion+Boscalid preventive effect × institute's accounting in embodiment
Example）×100
b:Synergistic effect %=（Embodiment prevention effect-theory preventive effect）/ theory preventive effect × 100
Begin to carry out dispenser with the composition of the present invention when grey mould fruit rot of strawberry first meeting scab as can be seen from Table 1, at interval of
10d is sprayed once, after continuously spraying 2 times, and 7 example of formulations of the present composition are preferable to the preventive effect of grey mould fruit rot of strawberry,
Except embodiment 2, preventive effect is more than 85%, and the particularly preventive effect of embodiment 7 reaches 93.10%, hence it is evident that higher than comparison medicament, preventive effect
Significantly；The present composition is compared with single dose, has synergistic effect, particularly embodiment 2,3 and the synergistic effect of embodiment 6 to be up to
More than 20%, synergistic effect substantially, is remarkably improved prevention effect；The present composition grows safety to strawberry, and field is not found
Any adverse reaction, so said composition can be widely used in the preventing and treating of Field strawberries gray mold in production.
Claims (7)
1. a kind of bactericidal composition containing bacillus amyloliquefaciens and Boscalid, it is characterised in that said composition is to solve starch bud
Spore bacillus and Boscalid are active component, and its mass ratio is 10:1~1:10, optimum ratio is 4:1~1:4.
2. the composition according to claim 1 containing bacillus amyloliquefaciens and Boscalid, it is characterised in that：Described
The entitled WH1G of bacillus amyloliquefaciens, preservation number is CGMCC NO. 10722.
3. the composition containing bacillus amyloliquefaciens and Boscalid according to claim 1,2, it is characterised in that described
Bacillus amyloliquefaciens in spore count living be 1,000,000,000/gram ~ 10,000,000,000/gram.
4. the composition containing bacillus amyloliquefaciens and Boscalid according to claim 1,2,3, it is characterised in that to
Added in active component bacillus amyloliquefaciens and Boscalid after auxiliary agent and filler, you can with the agriculture that must be adapted to agriculturally use
With formulation, such as wettable powder and aqueous suspension agent.
5. the auxiliary agent used can be each from wetting agent, dispersant, bleeding agent, antifreezing agent, thickener, synergist, defoamer etc.
One or more in analog assistant;The filler used can be precipitated calcium carbonate, white carbon, kaolin, attapulgite, organic bentonite
One or more in profit soil.
6. the composition according to claim 4 containing bacillus amyloliquefaciens and Boscalid, it is characterised in that wherein have
Effect composition accounts for the 20% ~ 60% of bactericidal composition gross weight, and auxiliary agent accounts for the 10% ~ 30% of bactericidal composition gross weight, and remaining is filler.
7. the application of the composition according to claim 5 containing bacillus amyloliquefaciens and Boscalid, it is characterised in that
Prevented and treated in grey mould fruit rot of strawberry early period of origination using spray-on process.
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Cited By (1)
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| CN110622997A (en) * | 2019-11-08 | 2019-12-31 | 四川龙蟒福生科技有限责任公司 | Bactericidal composition, application thereof and bactericide |
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