KR101810224B1 - Natural fertilizer compositions for crops comprising seaweed - Google Patents
Natural fertilizer compositions for crops comprising seaweed Download PDFInfo
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- KR101810224B1 KR101810224B1 KR1020170048150A KR20170048150A KR101810224B1 KR 101810224 B1 KR101810224 B1 KR 101810224B1 KR 1020170048150 A KR1020170048150 A KR 1020170048150A KR 20170048150 A KR20170048150 A KR 20170048150A KR 101810224 B1 KR101810224 B1 KR 101810224B1
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
- C05F1/005—Fertilisers made from animal corpses, or parts thereof from meat-wastes or from other wastes of animal origin, e.g. skins, hair, hoofs, feathers, blood
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/02—Other organic fertilisers from peat, brown coal, and similar vegetable deposits
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G1/00—Mixtures of fertilisers belonging individually to different subclasses of C05
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
Description
본 발명은 해조류를 포함하는 농작물용 천연 영양제 조성물에 관한 것이다. The present invention relates to natural nutrient compositions for crops, including seaweeds.
농작물은 사람의 보호 및 관리 하에서 군락을 이루고 생육하는 식물을 의미하는데, 각종 채소류와 같은 식용작물 외에 꽃 등의 관상작물도 포함된다.Crops means plants that grow and grow in a community under the protection and management of humans. In addition to edible crops such as various vegetables, corn crops such as flowers are also included.
산업의 급속한 발달과 양질의 농산물에 대한 수요증대 및 생산성 향상을 위하여 화학비료와 농약이 토지에 광범위하게 과다 사용되고 있다. 이러한 농후 화학비료와 농약에 편중된 관행농법은 토양의 측면에 있어서, 지력 저하와 토양의 산성화를 야기시키고 있다. 또한, 최종 분해자인 미생물 및 토양생물계를 억제하는 결과를 가져와 농토를 점차 황폐화시키는 주범이 되고 있다. 이뿐만 아니라 식물의 생육도중에 발생되는 병충해를 방제하기 위한 농약의 과다사용은 병원균의 방제제에 대한 내성의 증가 외에도 토양 내 유용미생물상의 단순화 및 억제를 유발하였으며, 이는 결국 토양 병원균과 유해 선충 밀도의 급격한 증가로 이어져, 농약을 대량 사용하여야 하는 악순환을 초래하였다.Chemical fertilizers and pesticides have been extensively used in land extensively for rapid development of industry, increase in demand for high quality agricultural products, and improvement of productivity. Conventional farming methods concentrated on such rich chemical fertilizers and pesticides cause soil degradation and soil acidification on the side of the soil. In addition, the result of inhibiting microorganisms and soil biosystems, which are the final decomposers, is becoming a major cause of devastation of agricultural land. In addition, overuse of pesticides to control insects caused during the growth of plants caused simplification and inhibition of beneficial microorganisms in soil, in addition to the increase of tolerance to pathogens, which resulted in soil pathogens and nematode densities Leading to a vicious cycle in which a large amount of pesticides must be used.
또한, 농작물에 측면에 있어서 농후 화학비료와 농약은 심각한 염류집적 현상을 유발하고, 이로 인한 영양분간의 길항작용으로 미네랄 결핍 등에 의한 연쇄적인 장애가 발생하여 농작물의 내병성이 저하되고, 생육불량으로 수확이 감소하며, 품질과 맛이 저하되는 등의 심각한 문제를 발생시켰다.In addition, in terms of crops, rich chemical fertilizers and pesticides cause severe salt accumulation phenomenon, and due to the antagonistic action between the nutrients caused by this, a series of troubles due to mineral deficiency occurs, resulting in deterioration of crop disease resistance, And serious problems such as degradation of quality and taste were caused.
최근에는 환경오염 문제와 함께 이러한 농작물 오염문제가 심각하게 대두되면서 화학비료나 농약을 사용하기보다는 양질의 유기질 비료나 퇴비를 사용하는 농법개발이 확산되어, 많은 유기질 비료의 개발이 지속적으로 시도되고 있다. In recent years, with the problem of environmental pollution, the pollution problem of such crops has been seriously raised. Therefore, rather than using chemical fertilizers or pesticides, the development of agricultural methods using high quality organic fertilizers or compost has spread, and many organic fertilizers have been continuously developed .
그러나 유기질 비료 및 퇴비를 사용하더라도, 재료학적으로만 차이가 있을 뿐 화학제와 비슷한 또 다른 문제점이 심화될 수 있다. However, even with organic fertilizers and composting, other problems similar to chemical agents can be exacerbated only by material differences.
그러므로, 식물의 생육을 촉진시킬 수 있는 영양분을 제공할 뿐 아니라, 토양 자체의 특성이나 미생물 활성 등에 바람직한 영향을 제공할 수 있는 천연 영양제의 개발이 요구되고 있다. Therefore, there is a demand for the development of natural nutrients that not only provide nutrients that can promote the growth of plants, but also can provide a favorable effect on soil characteristics or microbial activity.
본 발명은, 종래기술의 상기와 같은 문제를 해결하기 위하여 안출된 것으로서,SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems of the prior art,
토양의 산성화, 토양 경화(硬化) 등의 부작용이 없이, 농작물에 대한 충분한 영양분과 우수한 살균효과를 제공할 수 있는 농작물용 천연 영양제 조성물을 제공하는 것을 목적으로 한다.It is an object of the present invention to provide a natural nutrient composition for agricultural crops which can provide sufficient nutrients and excellent germicidal effect on crops without side effects such as soil acidification and soil hardening.
본 발명은, 상기 목적을 달성하기 위하여, The present invention, in order to achieve the above object,
해조류 10~30 중량부, 닭부산물로 제조된 겔화물 5~15 중량부, 조릿대잎 1~5 중량부, 및 게껍질분말 1~5 중량부를 포함하는 혼합물에 바실러스 서브틸리스(Bacillus subtilis), 바실러스써린지엔시스(Bacillus thuringiensis), 바실러스 투린지엔시스(Bacillus thuringiensis), 유산균, 광합성세균, 효모균, 그람양성의 방선균 및 곰팡이균 중에서 선택되는 1종 이상의 미생물을 접종하고, 3~20일간 발효시켜서 제조된 농작물용 천연 영양제 조성물을 제공한다.10 to 30 parts by weight of seaweed, 5 to 15 parts by weight of gelatin produced as a by-product of chicken, 1 to 5 parts by weight of sage leaves, and 1 to 5 parts by weight of crab shell powder are added to a mixture of Bacillus subtilis, The microorganism is inoculated with at least one microorganism selected from Bacillus thuringiensis, Bacillus thuringiensis, lactic acid bacteria, photosynthetic bacteria, yeast bacteria, gram-positive actinomycetes and fungi, and fermented for 3 to 20 days Natural nutrient composition for agricultural crops.
본 발명의 다른 실시형태에 있어서, 상기 혼합물은 고삼 뿌리 1~5 중량부, 창포 1~5 중량부, 및 흰목이버섯 1~5 중량부를 더 포함하여 혼합된 것일 수 있다. In another embodiment of the present invention, the mixture may further comprise 1 to 5 parts by weight of roots, 1 to 5 parts by weight of iris, and 1 to 5 parts by weight of white mushroom.
상기 해조류는 우뭇가사리, 해태, 미역, 및 다시마로 이루어진 군으로부터 선택되는 1종 이상일 수 있다. The seaweeds may be at least one species selected from the group consisting of marigolds, seabass, seaweed, and kelp.
본 발명의 다른 실시형태에 있어서, 상기 닭부산물로 제조된 겔화물은 In another embodiment of the present invention, the gelled product made from the chicken by-
닭부산물인 닭머리, 닭발, 닭껍질, 및 닭내장 중에서 선택되는 1종 이상을 5~20배의 물에 넣고, 75~100℃의 온도로 0.5~24 시간 동안 열수추출을 실시하는 단계;Adding at least one selected from the chicken byproducts selected from chicken head, chicken leg, chicken skin, and chicken intestine into 5 to 20 times of water and performing hot water extraction at a temperature of 75 to 100 ° C for 0.5 to 24 hours;
상기 열수추출에 의해 추출이 완료된 후, 필터로 여과하여 닭뼈를 제거하는 단계; 및Removing the chicken bone by filtering with a filter after the extraction is completed by the hot water extraction; And
상기 닭뼈가 제거된 추출물을 냉각시켜 겔화시키는 단계;를 통하여 제조된 것 일 수 있다. Cooling the extract from which the chicken bone has been removed and gelling it.
본 발명의 농작물용 천연 영양제 조성물은 토양의 산성화, 토양 경화(硬化) 등의 부작용이 없이, 농작물에 대한 충분한 영양분과 우수한 살균효과를 제공한다. The natural nutrient composition for crops of the present invention provides sufficient nutrients and excellent germicidal effect on crops without side effects such as soil acidification and soil hardening (curing).
이하에서 본 발명을 자세히 설명한다.Hereinafter, the present invention will be described in detail.
해조류 10~30 중량부, 닭부산물로 제조된 겔화물 5~15 중량부, 조릿대잎 1~5 중량부, 및 게껍질분말 1~5 중량부를 포함하는 혼합물에 바실러스 서브틸리스(Bacillus subtilis), 바실러스써린지엔시스(Bacillus thuringiensis), 바실러스 투린지엔시스(Bacillus thuringiensis), 유산균, 광합성세균, 효모균, 그람양성의 방선균 및 곰팡이균 중에서 선택되는 1종 이상의 미생물을 접종하고, 3~20일간 발효시켜서 제조된 농작물용 천연 영양제 조성물을 제공한다.10 to 30 parts by weight of seaweed, 5 to 15 parts by weight of gelatin produced as a by-product of chicken, 1 to 5 parts by weight of sage leaves, and 1 to 5 parts by weight of crab shell powder are added to a mixture of Bacillus subtilis, The microorganism is inoculated with at least one microorganism selected from Bacillus thuringiensis, Bacillus thuringiensis, lactic acid bacteria, photosynthetic bacteria, yeast bacteria, gram-positive actinomycetes and fungi, and fermented for 3 to 20 days Natural nutrient composition for agricultural crops.
본 발명의 다른 실시형태에 있어서, 상기 혼합물은 고삼 뿌리 1~5 중량부, 창포 1~5 중량부, 및 흰목이버섯 1~5 중량부를 더 포함하여 혼합된 것일 수 있다. In another embodiment of the present invention, the mixture may further comprise 1 to 5 parts by weight of roots, 1 to 5 parts by weight of iris, and 1 to 5 parts by weight of white mushroom.
본 발명의 다른 실시형태에 있어서, 상기 닭부산물로 제조된 겔화물은 In another embodiment of the present invention, the gelled product made from the chicken by-
닭부산물인 닭머리, 닭발, 닭껍질, 및 닭내장 중에서 선택되는 1종 이상을 5~20배의 물에 넣고, 75~100℃의 온도로 0.5~24 시간 동안 열수추출을 실시하는 단계;Adding at least one selected from the chicken byproducts selected from chicken head, chicken leg, chicken skin, and chicken intestine into 5 to 20 times of water and performing hot water extraction at a temperature of 75 to 100 ° C for 0.5 to 24 hours;
상기 열수추출에 의해 추출이 완료된 후, 필터로 여과하여 닭뼈를 제거하는 단계; 및Removing the chicken bone by filtering with a filter after the extraction is completed by the hot water extraction; And
상기 닭뼈가 제거된 추출물을 냉각시켜 겔화시키는 단계;를 통하여 제조된 것 일 수 있다. Cooling the extract from which the chicken bone has been removed and gelling it.
본 발명의 일실시 형태에 있어서, 상기 닭부산물인 닭머리, 닭발, 닭껍질, 및 닭내장은 7~3 : 5~1 : 4~1 : 4:1의 중량비로 포함하는 것이 경제성 및 영양성 면에서 바람직할 수 있다.In one embodiment of the present invention, the chicken by-products, chicken head, chicken leg, chicken skin, and chicken intestine are contained at a weight ratio of 7: 3: 5 to 1: 4 to 1: 4: Lt; / RTI >
이하에서 상기 천연 살충제 조성물물의 성분에 대하여 자세히 설명한다.The components of the natural pesticide composition will be described in detail below.
상기 해조류는 우뭇가사리, 해태, 미역, 다시마 등으로 이루어진 군으로부터 선택되는 1종 이상일 수 있다. 그러나, 상기 종류로 한정되는 것은 아니다.The seaweeds may be at least one species selected from the group consisting of mugwort, sea lion, seaweed, sea tangle and the like. However, the present invention is not limited to these types.
상기 조릿대잎은 천연 식이 유황성분을 포함하며, 항암, 기침을 멎게 하는 작용, 염증, 해독, 진정, 진통, 이뇨작용을 갖는 것으로 알려져 있다. 본 발명자들은 이 밖에 살균 및 살충에도 효과가 있음을 확인하였다.The bamboo leaves contain natural dietary sulfur components and are known to have anti-cancer, cough-stopping action, inflammation, detoxification, sedation, analgesia and diuretic action. The inventors of the present invention have also confirmed that they are effective also for sterilization and insecticide.
상기 고삼은 콩과식물로서, 항알레르기 활성이 크며, 이뇨작용, 자궁수축작용, 해열, 진통, 살충효과 등이 크다고 알려져 있다.The ginseng is a soybean plant and has a high antiallergic activity, and is known to have a great effect on diuretic action, uterine contraction, antipyretic, analgesic and insecticidal effects.
상기 창포는 약리실험에서 진정, 진통, 항경련 작용과 순환계통에 대한 작용, 진해거담 작용, 소화기에 대한 작용, 피부 미용에 관한 작용이 보고되어 있다. 본 발명자들은 이 밖에 살균 및 살충에도 효과가 있음을 확인하였다.The iris has been reported to have effects on calming, analgesic, anticonvulsant and circulatory system, action on the digestive tract, action on the digestive system, and skin care in pharmacological experiments. The inventors of the present invention have also confirmed that they are effective also for sterilization and insecticide.
상기 흰목이버섯(Tremella fuciformis)은 여름과 가을에 각종 활엽수의 죽은 나무 또는 나뭇가지에서 자란다. 자실체는 지름 3~8cm, 높이 2~5cm, 불규칙한 파형의 덩어리 모양 겹꽃 모양이다. 표면은 순백색, 평활하며, 자실층은 표면 전체에 발달한다. 조직은 반투명한 젤라틴질이다. 식용 버섯으로 면역 증진, 콜레스테롤 저하, 혈당강하, 항산화 작용이 있는 약용 버섯이며, 한방에서는 호흡기 질환, 위염, 고혈압, 월경 불순 등 여러 질환에 도움이 된다고 알려져 있다. 주요 성분으로는 비타민 B1, B2, D 및 니아신(niacin), 만니톨(mannitol), 글리세롤(glycerol), 글루코스(glucose), 트레할로스(trehalose), 헤미셀룰로우스(hemicellulose), 키틴(chitin), 펙틴(pectin), 루코노실로 만난(luconoxylomannan), 리그닌(lignin) 등이 알려져 있다. 본 발명자들은 이 밖에 살균 및 살충에도 효과가 있음을 확인하였다.The above-mentioned Tremella fuciformis grows in various hardwood dead trees or branches in summer and autumn. Fruiting body is 3 ~ 8cm in diameter, 2 ~ 5cm in height, lumpy shape with irregular wave shape. The surface is pure white, smooth, and the fascial layer develops throughout the surface. The tissue is translucent gelatinous. It is medicinal mushroom which has immunity enhancement, cholesterol lowering, blood sugar lowering, antioxidant effect by edible mushroom, and it is known that it is helpful in various diseases such as respiratory diseases, gastritis, hypertension and menstrual irregularity. The main components include vitamins B1, B2, D and niacin, mannitol, glycerol, glucose, trehalose, hemicellulose, chitin, pectin, (pectin), luconoxylomannan, lignin, and the like are known. The inventors of the present invention have also confirmed that they are effective also for sterilization and insecticide.
본 발명에 있어서, 상기 접종 미생물로는 바실러스 서브틸리스(Bacillus subtilis), 바실러스써린지엔시스(Bacillus thuringiensis), 바실러스 튜린지엔시스(Bacillus thuringiensis), 유산균, 광합성세균, 효모균, 그람양성의 방선균 및 곰팡이균 등의 혼합물을 사용할 수 있다. 상기 광합성세균으로는 시아노박테리아(Cyanobacteria) 등, 방선균으로는 스트렙토마이세스(Streptomycesspp.), 곰팡이균로는 아스퍼질러스 오리지(Aspergillus oryzae), 아스퍼질러스(Aspergillusspp.), 페니실리움(Penicillum) 등을 들 수 있다.In the present invention, the inoculating microorganisms include Bacillus subtilis, Bacillus thuringiensis, Bacillus thuringiensis, lactic acid bacteria, photosynthetic bacteria, yeast bacteria, gram-positive actinomycetes and fungi A mixture of bacteria and the like may be used. Examples of the photosynthetic bacteria include Cyanobacteria, Streptomyces sp. As actinomycetes, Aspergillus oryzae, Aspergillus spp., Penicillium spp. Penicillum) and the like.
상기 바실러스 섭틸리스(Bacillus subtilis)로는 한국과학기술연구원 부설 생명공학연구소 유전자은행에 기탁된 바실러스 섭틸리스 아밀로리쿼페이션스(KCTC 8913P) 또는 바실러스 섭틸리스 EBM 13(KCTC 0984BP)를 분양받아 사용한다.As the Bacillus subtilis, Bacillus subtilis amyloliquefaciens (KCTC 8913P) or Bacillus subtilis EBM 13 (KCTC 0984BP) deposited at the genetic bank of the Institute of Bioscience and Biotechnology Research Institute do.
상기 바실러스 튜린지엔시스(Bacillus thuringiensis)로는 한국생명공학연구원 유전자은행(KCTC)에 기탁된 균주 중 KCTC 1508 또는 KCTC1509 를 분양받아 사용할 수 있다. As the Bacillus thuringiensis, KCTC 1508 or KCTC1509 among the strains deposited in the KCTC of Korea Research Institute of Bioscience and Biotechnology can be used.
이하에서, 실시예를 통하여 본 발명을 보다 상세히 설명한다. 그러나, 하기의 실시예는 본 발명을 더욱 구체적으로 설명하기 위한 것으로서, 본 발명의 범위가 하기의 실시예에 의하여 한정되는 것은 아니다. 하기의 실시예는 본 발명의 범위 내에서 당업자에 의해 적절히 수정, 변경될 수 있다. Hereinafter, the present invention will be described in more detail by way of examples. However, the following examples are intended to further illustrate the present invention, and the scope of the present invention is not limited by the following examples. The following examples can be appropriately modified and changed by those skilled in the art within the scope of the present invention.
제조예Manufacturing example 1: One: 닭부산물로As a by-product of chicken 제조된 Manufactured 겔화물의Gelled 제조 Produce
닭부산물인 닭머리, 닭발, 닭껍질, 및 닭내장을 5:2:2:2의 중량비로 가열 용기에 넣고 10배의 물을 넣고, 100℃의 온도로 12 시간 동안 열수추출을 하였다. 상기 열수추에 의해 추출이 완료된 후, 나일론 필터(nylon filter)로 여과하여 닭뼈를 제거하였다. 상기 닭뼈가 제거된 추출물을 4 ℃로 3시간 동안 냉각시켜 겔화시켜서 닭부산물 겔화물을 제조하였다. Chicken by-products such as chicken head, chicken leg, chicken skin, and chicken breast were put into a heating container at a weight ratio of 5: 2: 2: 2, 10 times of water was added, and the mixture was subjected to hot water extraction at 100 ° C for 12 hours. After extraction was completed by the hot water weights, the chicken bone was removed by filtration with a nylon filter. The extract from which the chicken bone was removed was cooled at 4 캜 for 3 hours and gelated to prepare a chicken by-product gelled product.
실시예Example 1: 농작물용 천연 영양제 조성물의 제조 1: Preparation of natural nutrient composition for crops
해조류 25 중량부, 상기 제조예 1에서 제조된 닭부산물로 제조된 겔화물 8 중량부, 조릿대잎 3 중량부, 및 게껍질분말 3 중량부를 포함하는 혼합물에 바실러스 투린지엔시스(Bacillus thuringiensis) 균주로서 KCTC 1508 4 중량부를 접종하고 30℃에서 72 시간 배양하여 농작물용 천연 영양제 조성물을 제조하였다.25 parts by weight of seaweed, 8 parts by weight of gelatin prepared from the chicken by-product prepared in the above Preparation Example 1, 3 parts by weight of the sage leaves, and 3 parts by weight of the crab shell powder were added as a strain of Bacillus thuringiensis 4 parts by weight of KCTC 1508 were inoculated and cultured at 30 DEG C for 72 hours to prepare a natural nutrient composition for agricultural use.
실시예Example 2: 농작물용 천연 영양제 조성물의 제조 2: Preparation of natural nutrient composition for crops
해조류 25 중량부, 상기 제조예 1에서 제조된 닭부산물로 제조된 겔화물 8 중량부, 조릿대분말 3 중량부, 게껍질분말 3 중량부, 고삼뿌리 2중량부, 창포 2중량부, 및 흰목이버섯 2 중량부를 포함하는 혼합물에 바실러스 섭틸리스 아밀로리쿼페이션스 균주로서 KCTC 8913P 4 중량부를 접종하고 30℃에서 72 시간 배양하여 농작물용 천연 영양제 조성물을 제조하였다.25 parts by weight of seaweed, 8 parts by weight of gelatin prepared from the chicken byproduct prepared in Preparation Example 1, 3 parts by weight of sage powder, 3 parts by weight of crab shell powder, 2 parts by weight of ginseng root, 2 parts by weight of iris, And 2 parts by weight of mushroom were inoculated with 4 parts by weight of KCTC 8913P as Bacillus subtilis amyloliquefaciens strain and cultured at 30 DEG C for 72 hours to prepare a natural nutrient composition for crops.
시험예Test Example 1: 농작물용 천연 영양제 조성물의 식물 발아시간 및 성장속도 향상 효과 평가 1: Assessment of plant germination time and growth rate of natural nutrient composition for crops
본 발명에 따른 농작물용 천연 영양제 조성물의 식물 발아시간 및 성장속도 향상효과를 측정하였다. 실험방법은 황토판에 무순과 다채 씨앗을 골고루 뿌려 재배기에 설치한 다음 실온에서 7일간 배양하면서 발아시간과 생장속도를 관찰하였다.The plant germination time and growth rate of the natural nutrient composition for crops according to the present invention were measured. The germination time and the growth rate of the seedlings were investigated by cultivating the seedlings on the loess plate.
수돗물 공급Control group
Tap water supply
상기 표 1에서 보는 바와 같이, 본 발명의 실시예 1 및 실시예 2의 천연 영양제 조성물을 시비한 경우, 대조군과 비교하여 무순과 다채 모두에서 발아시간이 단축되고, 성장속도도 더 빠른 것을 확인하였다.As shown in Table 1, when the natural nutrient compositions of Examples 1 and 2 of the present invention were fertilized, the germination time was shortened and the growth rate was faster than that of the control group .
시험예Test Example 2: 농작물용 천연 영양제 조성물의 항균활성 효과 측정 2: Measurement of antimicrobial activity of natural nutrient composition for crops
본 발명의 농작물용 천연 영양제 조성물의 항균활성을 KICM-FIR-1002에 의거하여 실시하였다.The antimicrobial activity of the natural nutrient composition for agricultural use of the present invention was evaluated based on KICM-FIR-1002.
(CFU/40p)Initial concentration
(CFU / 40p)
(CFU/40p)Concentration after 24 hours
(CFU / 40p)
(%)Bacterial reduction rate
(%)
(실시예 1의 천연 영양제 조성물 함유)mortar
(Containing the natural nutrient composition of Example 1)
(실시예 2의 천연 영양제 조성물 함유)mortar
(Containing the natural nutrient composition of Example 2)
(실시예 1의 천연 영양제 조성물 함유)mortar
(Containing the natural nutrient composition of Example 1)
(실시예 2의 천연 영양제 조성물 함유)mortar
(Containing the natural nutrient composition of Example 2)
(주) (week)
1) 블랭크: 시료를 넣지 않은 상태에서 측정한 것임.1) Blank: Measured with no sample.
2) CFU: 콜로니 형성 단위(Colony Forming Uint)2) CFU: Colony forming unit (Colony Forming Uint)
3) 40p=0.04mL3) 40p = 0.04 mL
4) 배지상의 균수는 희석배수를 곱하여 산출한 것임.4) The number of bacteria on the medium multiplied by the dilution factor.
5) 모르타르 제작 후, 중성화하여 시험하였음5) After mortar making, it was tested by neutralization.
상기 표 2에 나타난 바와 같이, 대장균(Escherichia coli ATCC 25922)에 있어서는 대조군에 비해 각각 99.3%(실시예 1), 99.8%(실시예 2)의 살균효과를 보였고, 녹농균(Pseudomonas aeruginosa ATCC 15442)의 경우 각각 99.4%(실시예 1), 99.7%(실시예 2)의 살균효과를 보였다. As shown in the above Table 2, Escherichia coli ATCC 25922 showed 99.3% (Example 1) and 99.8% (Example 2) bactericidal effect and 99.8% (Pseudomonas aeruginosa ATCC 15442) , 99.4% (Example 1) and 99.7% (Example 2), respectively.
상기 실험결과에 의하면, 본 발명의 천연 영양제 조성물은 항균 활성이 우수하여 재배작물에 살포할 경우 세균에 의한 식물병 예방의 효과를 기대할 수 있음을 알 수 있다.According to the experimental results, the natural nutritional composition of the present invention is excellent in antimicrobial activity, so that it can be expected that the effect of preventing bacteria caused by bacteria can be expected when the natural nutrient composition is sprayed on cultivated crops.
시험예Test Example 3: 농작물용 천연 영양제 조성물의 항균활성 효과 측정 3: Measurement of antimicrobial activity of natural nutrient composition for crops
본 발명의 농작물용 천연 영양제 조성물을 토양에 처리한 후 토질 개량효과를 측정하였다. 실험결과, 산성화를 나타내던 토양이 본 발명의 농작물용 천연 영양제 조성물 처리 후, 알카리화 되어 친환경 작물의 재배에 적합한 토양으로 개량되었음을 확인할 수 있었으며, 토양에 반복적으로 살포하여도 토양의 경화(硬化)현상이 발생하지 않는 것을 확인할 수 있었다.After the natural nutrient composition for crops of the present invention was treated on the soil, the soil improvement effect was measured. As a result of the experiment, it was confirmed that the soil showing acidification was treated with the natural nutrient composition for crops of the present invention and then alkalized to improve the soil suitable for cultivation of environmentally friendly crops. Even when repeatedly sprayed on the soil, It was confirmed that the phenomenon did not occur.
Claims (4)
상기 닭부산물로 제조된 겔화물은,
닭부산물인 닭머리, 닭발, 닭껍질, 및 닭내장 중에서 선택되는 1종 이상을 5~20배의 물에 넣고, 75~100℃의 온도로 0.5~24 시간 동안 열수추출을 실시하는 단계;
상기 열수추출에 의해 추출이 완료된 후, 필터로 여과하여 닭뼈를 제거하는 단계; 및
상기 닭뼈가 제거된 추출물을 냉각시켜 겔화시키는 단계;를 통하여 제조된 것을 특징으로 하는 농작물용 천연 영양제 조성물.10 to 30 parts by weight of seaweed, 5 to 15 parts by weight of gelatin produced as a by-product of chicken, 1 to 5 parts by weight of sage leaves, and 1 to 5 parts by weight of crab shell powder are added to a mixture of Bacillus subtilis, The microorganism is inoculated with at least one microorganism selected from Bacillus thuringiensis, Bacillus thuringiensis, lactic acid bacteria, photosynthetic bacteria, yeast bacteria, gram-positive actinomycetes and fungi, and fermented for 3 to 20 days However,
The gelled product made from the chicken by-
Adding at least one selected from the chicken byproducts selected from chicken head, chicken leg, chicken skin, and chicken intestine into 5 to 20 times of water and performing hot water extraction at a temperature of 75 to 100 ° C for 0.5 to 24 hours;
Removing the chicken bone by filtering with a filter after the extraction is completed by the hot water extraction; And
And cooling the extract from which the chicken bone has been removed to gelate the nutritional composition.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108409496A (en) * | 2018-05-04 | 2018-08-17 | 李小婷 | A kind of composite fertilizer and preparation method thereof preparing improvement soil using dead leaf |
| CN108440198A (en) * | 2018-05-08 | 2018-08-24 | 徐学 | A kind of composite fertilizer suitable for acid soil |
| WO2023224228A1 (en) * | 2022-05-18 | 2023-11-23 | 농업회사법인 유한회사 제주테라 | Fertilizer composition for plant cultivation, comprising fermented solution of seaweed, and preparation method therefor |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108409496A (en) * | 2018-05-04 | 2018-08-17 | 李小婷 | A kind of composite fertilizer and preparation method thereof preparing improvement soil using dead leaf |
| CN108440198A (en) * | 2018-05-08 | 2018-08-24 | 徐学 | A kind of composite fertilizer suitable for acid soil |
| WO2023224228A1 (en) * | 2022-05-18 | 2023-11-23 | 농업회사법인 유한회사 제주테라 | Fertilizer composition for plant cultivation, comprising fermented solution of seaweed, and preparation method therefor |
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