CN101953377A - Bacterial biological fungicide and preparation method thereof - Google Patents
Bacterial biological fungicide and preparation method thereof Download PDFInfo
- Publication number
- CN101953377A CN101953377A CN200910181308XA CN200910181308A CN101953377A CN 101953377 A CN101953377 A CN 101953377A CN 200910181308X A CN200910181308X A CN 200910181308XA CN 200910181308 A CN200910181308 A CN 200910181308A CN 101953377 A CN101953377 A CN 101953377A
- Authority
- CN
- China
- Prior art keywords
- fungicide
- bacterium
- pseudomonas putida
- preparation
- living beings
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a bacterial biological fungicide and a preparation method thereof. Microbial pesticide living Pseudomonas putida is used as a starting strain; and the bacterial biological fungicide is obtained after fermentation and reproduction. The bacterial biological fungicide can be used for nearly all crops infected by Pythium, Rhizoctonia and Fusarium, is safe for the crops, and has good effect on the crops such as cucumber, tomato, scallion, eggplant, green pepper, cotton, watermelon, sesame and the like. The bacterial biological fungicide has the greatest advantages that the bacterial biological fungicide is all nature, harmless, safe to people and livestock and environment-friendly; the pathogenic bacteria are not easy to produce resistance; and the bacterial biological fungicide is favorable for production of green vegetables, and has huge economic, ecological and social benefits.
Description
Technical field
The present invention relates to the microbial pesticide field, relate in particular to a kind of bacterium living beings fungicide and its preparation method, be mainly used in control by pythium Pythium, Rhizoctonia Rhizoctonia or the caused vegetables of Fusarium Fusarium fungal pathogens and other crop root disease.
Background technology
The vegetables root disease is the most obstinate vegetable disease, causes vegetables to drop in production over a large area throughout the year; In the prior art, the vegetable grower mainly uses such disease of chemical pesticide control, because long-term use will directly cause a series of ecologies and social concerns such as water source and environmental pollution, agricultural product residually exceed standard, biological chain interruption, soil degradation, disruption of ecological balance; And chemical pesticide is carcinogenic, teratogenesis, mutagenic effect are causing showing great attention to of scientific circles.
Along with the raising of people's living standard, increasing to demand green, pollution-free food, the hypertoxic chemical pesticide market of will progressively fading out, safety, efficient, economic and pesticide species easy to use will become the main product in market; In order to ensure food security, promote a conservation culture, be badly in need of exploring an eco-friendly extermination of disease and insect pest new way.Pseudomonas putida (Pseudomonas putida) is a kind of gram-negative rod bacterium, its metabolic pathway is very extensive, be mainly used in the hazardous compound that decomposes in the environment, known several Pseudomona sputida fungus strains are that very outstanding plant growing promotes bacterium, but yet there are no the Pseudomonas putida fungus strain that report is used for controlling plant diseases.
Summary of the invention
The objective of the invention is, the problem that exists at prior art provides a kind of bacterium living beings fungicide and its preparation method, has solved and has used the residual problem such as exceed standard of the existing crops of chemical pesticide in the prior art.
Technical scheme of the present invention is as follows:
A kind of bacterium living beings fungicide that is used to prevent and treat the crop root part pathogen, it is characterized in that: comprise following components in weight percentage: pseudomonas putida 1-2%, surfactant 9-15%, the liquid-carrier inserts accounts for 83-90%, and each constituent content percentage sum is 100%.
Described bacterium living beings fungicide, wherein: described inserts is a white carbon.
A kind of preparation method who is used to prevent and treat the bacterium living beings fungicide of crop root part pathogen is characterized in that: may further comprise the steps:
A, to the pseudomonas putida separation of ruling, the unicellular purebred bacterial strain of the described pseudomonas putida that obtains is carried out amplification culture, obtain kind of a daughter bacteria liquid;
B, described kind of daughter bacteria liquid that will obtain insert and contain in the fermentation tank of microbial culture medium, control and regulate temperature in the described fermentation tank and be 30 ℃, pH value and be 7 and carry out 120 hours submerged fermentation;
C, cell density reaches 10 in zymotic fluid
11During CFU/ml, to the culture fluid after the fermentation filter, centrifugal, the dry former powder of bacterium that obtains dusts;
D, employing percentage by weight are the former powder of described bacterium of 1%-2%, add surfactant 9-15%, and liquid-carrier inserts 83-90% is ground to 400 order fineness and obtains bacterium living beings fungicide behind the mixing.
Described preparation method, wherein, the culture fluid among the described step B comprises: corn flour 2.0%, soybean cake powder 3.5%, fish meal 2.0%, groundnut meal 2.0%, starch 1.0%, potassium dihydrogen phosphate 0.3%, calcium carbonate 0.3%.
Described preparation method, wherein, described inserts is a white carbon.
Beneficial effect of the present invention is: utilize microbial pesticide live body Pseudomona sputida to be starting strain, by obtaining bacterium living beings fungicide behind the fermentating breeding, can be used to be subjected to rotten mold genus Pythium, on nearly all crop that Rhizoctonia Rhizoctonia and Fusarium Fusarium infect, and to crops safety, cucumber, tomato, green onion, eggplant, green pepper, cotton, good effect is all arranged on the crop such as watermelon and sesame, its biggest advantage is exactly a pure natural, nuisanceless, to the person poultry safety, free from environmental pollution, and pathogen is difficult for producing resistance, help the production of green non-pollution vegetables, have huge economy, ecology and social benefit.
Description of drawings
Fig. 1 is a bacterium living beings fungicide production process schematic diagram of the present invention.
Embodiment
The invention provides a kind of bacterium living beings fungicide and its preparation method that is used to prevent and treat the crop root part pathogen, for making purpose of the present invention, technical scheme and advantage clearer, clear and definite, below develop simultaneously with reference to accompanying drawing that the present invention is described in more detail for embodiment.
The present invention utilizes microbial pesticide live body Pseudomonas putida, the Chinese name pseudomonas putida, it is a kind of gram-positive rod bacterium, its metabolic pathway is very extensive, be mainly used in the hazardous compound that decomposes in the environment, known several Pseudomonas putida fungus strains are that very outstanding plant growing promotes bacterium, but yet there are no the Pseudomonas putida fungus strain that report is used for controlling plant diseases, the present invention utilizes this microbial pesticide live body to add other reagent acquisition bacterium living beings fungicide for the strain fermentation breeding back of setting out just and prevents and treats by pythium Pythium, Rhizoctonia Rhizoctonia or the caused vegetables of Fusarium Fusarium fungal pathogens and other crop root disease.
Shown in 1, the method for preparing bacterium living beings fungicide of the present invention comprises with reference to the accompanying drawings:
A, the microbial pesticide live body Pseudomonas putida that at first low temperature preserved are as the bacterial classification that sets out, and line separates on plating medium, the unicellular purebred bacterial strain of acquisition Pseudomonas putida;
B, the unicellular purebred bacterial strain of 1000 milliliters of Pseudomonas putida that will obtain are put into 500 liters of seeding tanks, carry out amplification culture 48 hours, obtain kind of a daughter bacteria liquid;
C, 500 liters of kind daughter bacteria liquid are inserted filling in 10 tons of fermentation tanks of microbial culture medium, is that 30 ℃, pH value are 7 and carry out 120 hours submerged fermentation by being installed in pH value adjuster and the temperature controller control on the fermentation tank and regulating temperature in the fermentation tank; Wherein: medium (liquid) is corn flour 2.0%, soybean cake powder 3.5%, fish meal 2.0%, groundnut meal 2.0%, starch 1.0%, potassium dihydrogen phosphate (K2HPO4) 0.3%, calcium carbonate (CaCO3) 0.3%, and the solid content of whole medium is 11.1%;
D, cell density reaches 10 in zymotic fluid
11During CFU/ml, to the culture fluid after the fermentation filter, centrifugal, the dry former powder of bacterium that obtains dusts;
E, from the dry former powder of bacterium that obtains that dusts by following percentage by weight set of dispense ratio: the former powder of pseudomonas putida bacterium of 1%-2%, surfactant 9-15%, liquid-carrier inserts 83-90%, be ground to 400 order fineness behind the mixing and obtain bacterium living beings fungicide, and be packaged into the agricultural chemicals finished product; Wherein, preferred embodiment can be by the former powder 1.15% of pseudomonas putida bacterium, surfactant 10%, and the proportioning of liquid-carrier inserts 88.85% obtains bacterium living beings fungicide, and the liquid-carrier inserts can be selected white carbon.
The Pseudomonas putida bacterial classification that the present invention adopts, fruit has extremely outstanding diseases prevention, cures the disease and urgees to come into force, it is by several different mechanisms controls and treatment vegetables root disease, its advantage is: after being used to plant root, this beneficial microbe more has the advantage of contention nutrient source than pathogenic microorganisms, can around root, form beneficial microorganism advantage group faster, form layer protecting film, suppress in the growing environment harmful bacterium grow breeding, prevent and reduce the generation of various mushroom diseases; Pseudomonas putida has chemotaxis, energy active attack phytopathogen, the flagellum of dependence itself is followed the trail of the signaling molecule that disease fungus discharges, in case contact with the pathogen mycelia, can produce the antibiotic material, effectively kill and suppress the various plants pathogen, suppress the breeding of pathogen in the soil and the invasion and attack of plant root, reduce plant soil-borne diseases, the outburst of prevention multiple diseases; Can improve the bud ratio and the survival rate of seedlings of seed, the hereditary disease of prevention seed self improves the crop survival rate, promotes root growth.This bacterium can reduce the ethrel content that suppresses growth in the plant corpus effectively, and can decompose organic substance in the soil, improves fertilizer efficiency; More can improve soil aggregate, improve soil water storage, accumulation of energy and ground temperature, alleviate the continuous cropping obstacle; Under minimizing fertilizer amount 75% condition, still can reach even increase yield of vegetables.Its application process is that this product and water are mixed method and the earth mixture of back with the low pressure spraying, and application dosage is 115-220g/M
3Soil, rhizome and seed can be before plantation directly dipping concentration be the bacterium liquid of 120g/L, to by pythium Pythium, Rhizoctonia Rhizoctonia or the caused root disease control efficiency of Fusarium Pythium pathomycete at 80-90%.This product can not only promote plant growth, reduces cost, improve the yield and quality, and can prolong the continuous picking time of crop, thereby can greatly increase the crop output value, has apparent economic well-being of workers and staff, also has extremely good social benefit and ecological benefits.Following table is that the present invention uses the drug effect of Pseudomonas putida bacterial classification and other chemical pesticide commonly used and cost to compare:
It should be noted that, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although the present invention is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement technical scheme of the present invention, and not breaking away from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (5)
1. bacterium living beings fungicide that is used to prevent and treat the crop root part pathogen, it is characterized in that: comprise following components in weight percentage: pseudomonas putida 1-2%, surfactant 9-15%, the liquid-carrier inserts accounts for 83-90%, and each constituent content percentage sum is 100%.
2. bacterium living beings fungicide according to claim 1 is characterized in that: described inserts is a white carbon.
3. preparation method who is used to prevent and treat the bacterium living beings fungicide of crop root part pathogen is characterized in that: may further comprise the steps:
A, to the pseudomonas putida separation of ruling, the unicellular purebred bacterial strain of the described pseudomonas putida that obtains is carried out amplification culture, obtain kind of a daughter bacteria liquid;
B, described kind of daughter bacteria liquid that will obtain insert and contain in the fermentation tank of microbial culture medium, control and regulate temperature in the described fermentation tank and be 30 ℃, pH value and be 7 and carry out 120 hours submerged fermentation;
C, cell density reaches 10 in zymotic fluid
11During CFU/ml, to the culture fluid after the fermentation filter, centrifugal, the dry former powder of bacterium that obtains dusts;
D, employing percentage by weight are the former powder of described bacterium of 1%-2%, add surfactant 9-15%, and liquid-carrier inserts 83-90% is ground to 400 order fineness and obtains bacterium living beings fungicide behind the mixing.
4. preparation method according to claim 3 is characterized in that: the culture fluid among the described step B comprises: corn flour 2.0%, soybean cake powder 3.5%, fish meal 2.0%, groundnut meal 2.0%, starch 1.0%, potassium dihydrogen phosphate 0.3%, calcium carbonate 0.3%.
5. preparation method according to claim 3 is characterized in that: described inserts is a white carbon.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910181308XA CN101953377A (en) | 2009-07-17 | 2009-07-17 | Bacterial biological fungicide and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910181308XA CN101953377A (en) | 2009-07-17 | 2009-07-17 | Bacterial biological fungicide and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101953377A true CN101953377A (en) | 2011-01-26 |
Family
ID=43481171
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200910181308XA Pending CN101953377A (en) | 2009-07-17 | 2009-07-17 | Bacterial biological fungicide and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101953377A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559512A (en) * | 2011-12-16 | 2012-07-11 | 北京市农林科学院 | Fusarium oxysporum tomato neck rot root rot specialization type and application thereof in cultivation of tomato neck rot rood rot disease-resistant varieties |
| CN105543152A (en) * | 2016-02-24 | 2016-05-04 | 长治学院 | Pseudomonas putida and application thereof to promotion of growth of cercidiphyllum japonicum |
| CN106170207A (en) * | 2014-01-31 | 2016-11-30 | 农业生物群落股份有限公司 | Modified biocontrol agent and application thereof |
| US10278397B2 (en) | 2014-01-31 | 2019-05-07 | AgBiome, Inc. | Modified biological control agents and their uses |
| RU2755282C2 (en) * | 2015-10-14 | 2021-09-14 | Байер Кропсайенс Лп | Axm1554 gene encoding delta-endotoxin and methods for its application |
| CN114317549A (en) * | 2022-01-08 | 2022-04-12 | 河南大学 | Application of muscarinic C-type acetylcholine receptor in prevention and treatment of migratory locust |
| CN116671525A (en) * | 2023-02-27 | 2023-09-01 | 云南微态源生物科技有限公司 | Liquid composite microorganism ferment and preparation method thereof, liquid biological agent and application thereof |
-
2009
- 2009-07-17 CN CN200910181308XA patent/CN101953377A/en active Pending
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559512A (en) * | 2011-12-16 | 2012-07-11 | 北京市农林科学院 | Fusarium oxysporum tomato neck rot root rot specialization type and application thereof in cultivation of tomato neck rot rood rot disease-resistant varieties |
| US10575529B2 (en) | 2014-01-31 | 2020-03-03 | AgBiome, Inc. | Modified biological control agents and their uses |
| CN106170207B (en) * | 2014-01-31 | 2019-12-27 | 农业生物群落股份有限公司 | Modified biological control agents and uses thereof |
| US11760971B2 (en) | 2014-01-31 | 2023-09-19 | AgBiome, Inc. | Modified biological control agents and their uses |
| US10278397B2 (en) | 2014-01-31 | 2019-05-07 | AgBiome, Inc. | Modified biological control agents and their uses |
| US10334855B2 (en) | 2014-01-31 | 2019-07-02 | AgBiome, Inc. | Modified biological control agents and their uses |
| US10508280B2 (en) | 2014-01-31 | 2019-12-17 | AgBiome, Inc. | Modified biological control agents and their uses |
| CN106170207A (en) * | 2014-01-31 | 2016-11-30 | 农业生物群落股份有限公司 | Modified biocontrol agent and application thereof |
| US11518977B2 (en) | 2014-01-31 | 2022-12-06 | AgBiome, Inc. | Modified biological control agents and their uses |
| RU2755282C2 (en) * | 2015-10-14 | 2021-09-14 | Байер Кропсайенс Лп | Axm1554 gene encoding delta-endotoxin and methods for its application |
| CN105543152A (en) * | 2016-02-24 | 2016-05-04 | 长治学院 | Pseudomonas putida and application thereof to promotion of growth of cercidiphyllum japonicum |
| CN105543152B (en) * | 2016-02-24 | 2018-10-23 | 长治学院 | A kind of pseudomonas putida and its application in promoting katsura tree growth |
| CN114317549A (en) * | 2022-01-08 | 2022-04-12 | 河南大学 | Application of muscarinic C-type acetylcholine receptor in prevention and treatment of migratory locust |
| CN114317549B (en) * | 2022-01-08 | 2022-08-19 | 河南大学 | Application of muscarinic C-type acetylcholine receptor in prevention and treatment of migratory locust |
| CN116671525A (en) * | 2023-02-27 | 2023-09-01 | 云南微态源生物科技有限公司 | Liquid composite microorganism ferment and preparation method thereof, liquid biological agent and application thereof |
| CN116671525B (en) * | 2023-02-27 | 2023-11-24 | 云南微态源生物科技有限公司 | Liquid composite microorganism ferment and preparation method thereof, liquid biological agent and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103484384B (en) | Trichoderma atroviride preparation for preventing and controlling vegetable fungal diseases and preparation method of trichoderma atroviride preparation thereof | |
| CN101928186B (en) | Composite microbial fertilizer for controlling crop diseases and insect pests, and preparation method thereof | |
| KR101669599B1 (en) | Composition for eliminating odor and heavy metal comprising effective microorganism culture broth as effective component | |
| CN101875571B (en) | Method for preparing enhanced liquid microbial organic fertilizer | |
| CN104789490B (en) | A kind of complex microorganism decomposing microbial inoculum, preparation method and application | |
| CN104962490B (en) | A kind of Enteromorpha microbial bacterial agent and preparation method thereof | |
| CN101953377A (en) | Bacterial biological fungicide and preparation method thereof | |
| CN103820348A (en) | Plant growth-promoting bacteria and preparation method and application of fungicide | |
| CN102249753A (en) | Method for producing multifunctional bioorganic fertilizer and application of multifunctional bioorganic fertilizer | |
| CN101503708B (en) | Cultivation fermentation method of Bacillus coagulans antimycotics active substance | |
| CN102010825A (en) | Microorganism compound bactericide for controlling cucumber fusarium wilt and preparation method thereof | |
| KR101670651B1 (en) | Method for producing fermented liquid fertilizer of manure with reduced odor and heavy metal content using effective microorganism | |
| CN103952329A (en) | Bacillus vallismortis and application thereof | |
| CN102643148A (en) | Special biological disease-resistant vermin-proof culture medium for flowers and preparation method thereof | |
| CN103651582B (en) | Broad-spectrum composite microbial fungicide and preparation method thereof | |
| CN105439725A (en) | Paenibacillus polymyxa pesticide-fertilizer for farm onsite fermentation and applications thereof | |
| KR102496204B1 (en) | Liquid fertilizer manufacturing method of organic natural hormones for plant activation and promoting plant growth | |
| CN104311334A (en) | Composite improver for repairing heavy metal contaminated soil and preparation process | |
| CN104230580A (en) | Composite microbial disease/insect-resistant agent for green vegetables and manufacturing method thereof | |
| CN106465734A (en) | A kind of preventing and treating tobacco bacterial wilt fermentation liquid and preparation method thereof | |
| CN101974454B (en) | Degrading bacteria for pyrethroids insecticides and bactericide thereof | |
| CN103141519A (en) | Preparation method and application of anti-biological inoculants for controlling crop soil-borne diseases | |
| KR101810224B1 (en) | Natural fertilizer compositions for crops comprising seaweed | |
| CN108690817A (en) | A kind of multifunctional broad-spectrum complex micro organism fungicide and its preparation and application | |
| CN102173954B (en) | Preparation method of microbial-ecological foliar fertilizer promoting plant growth and controlling pest |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110126 |