CN102173954B - Preparation method of microbial-ecological foliar fertilizer promoting plant growth and controlling pest - Google Patents
Preparation method of microbial-ecological foliar fertilizer promoting plant growth and controlling pest Download PDFInfo
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- CN102173954B CN102173954B CN201110069142XA CN201110069142A CN102173954B CN 102173954 B CN102173954 B CN 102173954B CN 201110069142X A CN201110069142X A CN 201110069142XA CN 201110069142 A CN201110069142 A CN 201110069142A CN 102173954 B CN102173954 B CN 102173954B
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Abstract
The invention discloses a preparation method of a microbial-ecological foliar fertilizer promoting plant growth and controlling pest. The method comprises the following steps: separately cultivating bacillus, lactic acid bacteria, rhodopseudomonas palustris with the cell concentration of over 10 billion/ml and a bacteria liquid of yeast with the cell concentration of over 2 billion/ml; and mixing the mixed solution of the four kinds of probiotics with abamectin of a certain proportion to obtain the liquid microbial-ecological foliar fertilizer with the total cell concentration of over 8 billion/ml. The method disclosed by the invention has the advantages that: all strains adopted are edible probiotics of human beings, safe and nontoxic; abamectin is a macrolides insecticidal antibiotic agent, and has the characteristics of high efficiency, low toxicity, no pollution, lasting effect and the like; the prepared microbial-ecological foliar fertilizer integrates the advantages of probiotics in promoting plant growth and abamectin in efficient pest control; the microbial-ecological foliar fertilizer is diluted with water for 100 times and sprayed onto the leaf surfaces of the plant every other 7-10 days; and the microbial-ecological foliar fertilizer not only obviously increases the plant yield by over 30%, but also can control plant pest, thereby having important application value.
Description
Technical field
The invention belongs to biological technical field, a kind of utilize probiotic bacterium and Avrmectin preparation particularly are provided
the method of Promoting plant growth the micro-microecological foliar fertilizer of pest control.
Background technology
Foliage fertilizer refers to the fertilizer that imposes on plant leaf and can be absorbed utilization, because foliage fertilizer can directly enter in plant materials from leaf section, the metabolism of involved in plant and organic building-up process, therefore, in the time of a little less than growth and development of plants later stage Root Absorption ability, can make up in time required nutritive substance.Foliage fertilizer as emerging fertilizer representative, can improve the yield and quality of crop, and its research and application more and more have been subject to people's generally attention.
Foliage fertilizer originates from France the earliest, from botanist Grisb in 1884 FeSO
4solution has spread upon on the jaundice grape leave in order to since correcting the yellowtop caused because of iron deficiency, and foliage fertilizer apply in production practice and research has had significant progress.One of agrochemistry originator's Byccehro thinks, leaf goes in can be as root when absorbing water droplet nutritive substance and magnesium and potassium etc. being absorbed to plant.Before more than 200 years, China peasant of Zhejiang Province starts the rice application foliage fertilizer, has opened up the precedent of China's application foliage fertilizer.Middle 1980s, China started to research and produce the new foliar fertilizer that different sorts contains plant-growth regulator or farm chemical ingredients, made the function of foliage fertilizer more diversified.After nineteen nineties, some new foliar fertilizers application effect in the plantation of the cash crop such as watermelon, citrus and grape is very remarkable.Along with foliage fertilizer research and going deep into of applying, the type of foliage fertilizer is enriched constantly, and kind constantly increases, and from initial single type foliage fertilizer, develops into present multiclass composite foliage fertilizer.At present, the nutritious type foliage fertilizer of foliage fertilizer main Types, adjustment type foliage fertilizer, microbiocidal foliage fertilizer, single type microelement foliage fertilizer and composite foliage fertilizer etc., wherein microorganism foliage fertilizer is a dark horse, and becomes the outstanding person in foliage fertilizer.Microorganism foliage fertilizer contains microbial cells and metabolite thereof, can stimulate plant growth, promotes crop metabolism, alleviates and prevent and treat the generation of disease.But adopting probiotic bacterium and biological pesticide combines to produce aspect micro-microecological foliar fertilizer less than reporting.
Animal microecological formulation refers to use micro-Ecological Principle will be from the probiotic bacterium separated in animal body, the active bacteria formulation of cultivating through special process.Probiotic bacterium (probiotics) word is the earliest in order to describe " by a kind of material that can stimulate another kind of microorganism growth of microorganism secretion ".Within 1989, probiotic bacterium is defined as " being of value to animal host's viable bacteria feed additive by improving the intestinal microflora balance ".The latest definition that European food in 2002 and feed association have provided probiotic bacterium for " probiotic bacterium is the microorganism lived, by take in can to the host produce one or more special and through proving the functional health benefit ".Because probiotics can promote growth of animal, improve meat, egg and milk quality and be expected to replace microbiotic, become focus and the forward position of domestic and international research and application,
But adopt probiotic bacterium rare as the research report in leaf leaven field.
Avrmectin (avermectins) is streptomyces griseus in soil microorganisms
streptomyces avermitilisthe class that fermentation produces has desinsection, kills mite, ten hexa-atomic Macrocyclic lactone compounds of eelworm-killing activity.Avrmectin have tag, stomach toxicity and strong characteristics and the effect of seepage force, insects and mites is had and tags and the effect such as stomach toxicity.Avrmectin has very strong perviousness to plant leaf, can kill subepidermal insect, its mechanism of action is mainly to disturb the nervous physiology activity, stimulate to discharge arthropodan nerve conduction is had to inhibiting γ-aminobutyric acid, paralysis symptom appears after making various pests and medicament contacting, dead after 2-4 days.Avrmectin all has very strong inhibitory or killing effect to most parasitic nematodes and arthropods, becomes widely used biological pesticide.
At present, although adopt multiple probiotic bacterium to produce microorganism foliage fertilizer and adopt Avrmectin to prepare the biological pesticide aspect, more extensive research is all arranged, probiotic bacterium is mixed with Avrmectin as Promoting plant growth and pest control novel micro-microecological foliar fertilizer aspect do not have to report.
Summary of the invention
In order to address the above problem, the purpose of this invention is to provide the multiple probiotic bacterium of a kind of employing and Avrmectin produce a kind of can either Promoting plant growth, micro-microecological foliar fertilizer that again can the pest control safety non-toxic, by being sprayed on plant leaf surface, to reach the purpose that improves plant biomass and quality pest control.
Technical scheme of the present invention: a kind of method of utilizing probiotic bacterium and Avrmectin to produce micro-microecological foliar fertilizer, it is characterized in that, specifically comprise that following work step is rapid:
At first, cultivate separately respectively the acquisition cell concn and reach 10,000,000,000/ml above bacillus natto, milk-acid bacteria and Rhodopseudomonas palustris, and cell concn reaches the above saccharomycetic liquid culture of 2,000,000,000/ml;
Secondly, the culture of the bacillus natto that will to account for respectively volume percent be 15-25%, account for the milk-acid bacteria that volume percent is 15-25% culture, account for the Rhodopseudomonas palustris that volume percent is 25-45% culture, account for the saccharomycetic culture that volume percent is 15-25% and evenly mix, the Avrmectin that adds again percent weight in volume 0.2 ‰-2 ‰, can obtain total cell concn and reach the above micro-microecological foliar fertilizer of liquid high value of 8,000,000,000/ml
.
The invention has the advantages that: 1) probiotic bacterium bacterial classification used is all the edible probiotic bacteriums of the mankind, safety non-toxic.2) advantage of micro-microecological foliar fertilizer collection probiotic bacterium Promoting plant growth and the effective pest control of Avrmectin gathers together.3) Avrmectin is the tunning of soil microorganisms, has the characteristics of efficient, low toxicity, nuisanceless, lasting medicine.4) after within 7-10 days, being sprayed on plant leaf surface by 100 times of micro-microecological foliar fertilizer dilutions, not only obviously prevent and treat the generation of insect pest of the plant about 60 days, and significantly improve plant biomass and reach 30%.There is important using value in fields such as Organic food, vegetables, flowers, fruit, tealeaves and leaf tobacco production.
The accompanying drawing explanation
Fig. 1 obtains respectively cell concn figure in different probiotic culture in the preparation process of specific embodiments of the invention 1.
Fig. 2 obtains total cell concn figure of micro-microecological foliar fertilizer in the preparation process of specific embodiments of the invention 1.
Fig. 3 is the figure that micro-microecological foliar fertilizer of specific embodiments of the invention 1 production improves little growth of rape speed.
Fig. 4 is that micro-microecological foliar fertilizer that specific embodiments of the invention 1 are produced is prevented and treated the figure that little rape insect pest occurs.
embodiment
Below in conjunction with specific embodiment, technical scheme of the present invention is described further.
Principle of the present invention is: probiotic culture comprises mycetocyte and metabolite thereof, possesses the stimulating plant metabolism and growth, alleviates and prevents the disease generating function.Avrmectin be a kind of take stomach poison function as advocating peace, have concurrently action of contace poison Biocidal, kill mite, nematocides, it makes the insect paralysis lethal by hindering the transmission of pest movements nerve signal; There is efficient, low toxicity, nuisanceless, lasting medicine, be difficult for developing immunity to drugs, use without advantages such as cross resistances with other agricultural chemicals.Micro-microecological foliar fertilizer of producing after probiotic culture and Avrmectin are mixed according to a certain percentage, the advantage of probiotic bacterium and meta-bolites raising plant-growth and the effective pest control of Avrmectin is combined, can increase substantially the speed of growth and the resistance against diseases of plant, reduce the generation of insect pest simultaneously.
At first separately fermentation culture obtains cell concn and is respectively 18,200,000,000/ml bacillus natto, 15700000000/ml lactobacillus, the liquid culture of 10300000000/ml Rhodopseudomonas palustris and 2,800,000,000/ml S. cervisiae, as shown in Figure 1, by cell concn, be respectively 18,200,000,000/ml bacillus natto 100ml, 15700000000/ml lactobacillus 100ml, 10300000000/ml Rhodopseudomonas palustris 100ml and 2,800,000,000/ml S. cervisiae 100m are mixed, then add 0.5 ‰ Avrmectin, having obtained total cell concn is 117.5 hundred million/ml, micro-microecological foliar fertilizer that Avrmectin content is 0.5 ‰, as shown in Figure 2.The micro-microecological foliar fertilizer formed by 4 kinds of probiotic bacteriums with containing 0.5 ‰ Avrmectins that experimental group will be produced, after 100 times of dilute with waters, according to the method at interval of evenly being sprayed on little rape blade face 1 time in 7 days, use, control group sprays the water of same dose, treat that little growth of rape is got respectively in 40 days and spray to use and do not spray and use each 50 little rapes of micro-microecological foliar fertilizer to be weighed, result shows that spraying average every the weight of the little rape that uses micro-microecological foliar fertilizer is 137.4 grams, and do not spray average every the weight of the little rape that uses micro-ecological blade face, be only 100.7 grams, as shown in Figure 3, use the little rape increase output of micro-microecological foliar fertilizer to reach 36.4%.The Major Pests that affects little growth of rape is cabbage caterpillar and small cabbage moth, and this research adopts every ground (20m
2) 5 samplings of diagonal lines, 5 little rapes of every fixing investigation, amount to borer population amount alive (the every ground 20m before investigation on 25 little rapes of investigation
2the worm radix be no less than 100), control time is before foliage fertilizer sprays and sprays latter 1 day, 3 days and 7 days.Result shows, the number of pest of spraying the every ground of control group of same dose water rises to 163 from 104, and the number of pest of spraying the every ground of experimental group of foliage fertilizer is reduced to 16 from 107, and as shown in Figure 4, the pest control rate is up to 85.0%.
embodiment 2
At first separately fermentation culture obtains cell concn and is respectively 17,700,000,000/ml bacillus natto, 15,700,000,000/ml lactobacillus, 10,200,000,000/ml Rhodopseudomonas palustris and 2,500,000,000/ml S. cervisiae totally 4 kinds of liquid cultures, according to the ratio that respectively accounts for total volume percent 15%, 15%, 45% and 25%, mixed respectively, then add 0.2 ‰ Avrmectin, having obtained total cell concn is 102.3 hundred million/ml, micro-microecological foliar fertilizer that Avrmectin content is 0.2 ‰.
At first separately fermentation culture obtains cell concn and is respectively 19,000,000,000/ml bacillus natto, 16,300,000,000/ml lactobacillus, 10,500,000,000/ml Rhodopseudomonas palustris and 2,700,000,000/ml S. cervisiae totally 4 kinds of liquid cultures, according to the ratio that respectively accounts for total volume percent 25%, 25%, 35% and 15%, mixed respectively, then add 1.5 ‰ Avrmectin, having obtained total cell concn is 129.1 hundred million/ml, micro-microecological foliar fertilizer that Avrmectin content is 1.5 ‰.
Claims (1)
1. the preparation method of a Promoting plant growth the micro-microecological foliar fertilizer of pest control, is characterized in that, specifically comprises the following steps:
At first, cultivate separately respectively the acquisition cell concn and reach 10,000,000,000/ml above bacillus natto, milk-acid bacteria and Rhodopseudomonas palustris, and cell concn reaches the above saccharomycetic liquid culture of 2,000,000,000/ml;
Secondly, the culture of the bacillus natto that will to account for respectively volume percent be 15-25%, account for the milk-acid bacteria that volume percent is 15-25% culture, account for the Rhodopseudomonas palustris that volume percent is 25-45% culture, account for the saccharomycetic culture that volume percent is 15-25% and evenly mix, the Avrmectin that adds again percent weight in volume 0.2 ‰-2 ‰, can obtain total cell concn and reach the above liquid micro-ecological foliage fertilizer of 8,000,000,000/ml.
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| CN103319223B (en) * | 2012-03-19 | 2017-08-29 | 庆阳敦博科技发展有限公司 | A kind of Foliar fertilizer special for capsicum and plantation method thereof |
| CN102775233B (en) * | 2012-08-06 | 2014-09-03 | 定襄县神宇科技示范基地有限公司 | Novel insecticidal and antibacterial biofertilizer and preparation method thereof |
| CN103058716A (en) * | 2013-01-14 | 2013-04-24 | 广西穗宁化工集团有限公司 | Organic matter liquid suitable for being used as leaf fertilizer raw material |
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| CN101816254A (en) * | 2010-05-11 | 2010-09-01 | 沈阳化工学院 | Method for enhancing weak light resistance of cucumbers by spraying compound bacterial manure on foliage |
| CN101892157B (en) * | 2010-08-18 | 2011-08-17 | 孙廷震 | Composite microbial inoculum for preparing biological bacterial fertilizer and preparation method thereof |
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