CN108395998A - A kind of desinsection biological compound fungi agent and preparation method thereof - Google Patents
A kind of desinsection biological compound fungi agent and preparation method thereof Download PDFInfo
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- CN108395998A CN108395998A CN201710070386.7A CN201710070386A CN108395998A CN 108395998 A CN108395998 A CN 108395998A CN 201710070386 A CN201710070386 A CN 201710070386A CN 108395998 A CN108395998 A CN 108395998A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention belongs to field of agricultural microbial technology more particularly to a kind of biological compound fungi agent and preparation method thereof of desinsection.The desinsection biological compound fungi agent includes compound bacteria; strain protective agent and carrier, the compound bacteria are thuringiensis, Paecilomyces lilacinus, beauveria bassiana, pseudomonas fluorescens, streptomyces fradiae, bacillusmusilaginosiengineering, bacillus licheniformis and Streptomyces hygrospinosus.Insecticidal range of the present invention is wide, dosage is few, it is at low cost, the breeding of pernicious bacteria and pest can effectively be inhibited, the insect resistance capacity of plant can be improved, soil activation, anti-adversity and soil fertility can also be enhanced, improve Absorption and Utilization of Fertilizer rate, the survival rate of higher efficient bacterium can be also kept while keeping higher killing rate, extends the time-to-live of efficient bacterium.
Description
Technical field
The invention belongs to field of agricultural microbial technology more particularly to a kind of desinsection biological compound fungi agent and its preparation sides
Method.
Background technology
Soil is that crops are rely the material base of growth.The number of the soil organism, the power of soil fertilizer ability, soil
The quality of earth ventilation capacity and the balance of the soil liquid etc. have conclusive effect to the growth of plant.Soil is mainly by mine
Substance, organic matter and microorganism three parts composition, the activity of edaphon is extremely important to plant root nutrition, because
Beneficial microbe in soil directly participates in the formation of soil fertility, including the conversion of matter and energy in soil, humus
Formation and decomposition, the release of nutrient, the fixation of nitrogen etc., but pure quantity of useful microbe under natural conditions is inadequate, active force
Also limited.
Due to the harm of various phytopathogens, many crops is made to bring about great losses, less serious case's underproduction, severe one can cause absolutely
Production, directly affects the economic interests of peasant, and for controlling disease, peasant has to using fungicide, but the length of fungicide
Phase, a large amount of uses, can destroy soil texture, a large amount of applications of fungicide cause accumulation of the fungicide in plant, pass through food
Object, feed harmful to human and animal health.Therefore, effective and reasonable to use fungicide, it reduces environmental pollution, eliminated soil hardening
As an important topic of the pendulum in agricultural production.
Currently, the biological compound fungi agent agriculturally used is primarily present, insecticidal range is limited, killing rate is low, the survival of effective bacterium
The deficiencies of rate is low, the time-to-live is short.
Invention content
The technical problem to be solved in the present invention is to provide a kind of biological compound fungi agent and preparation method thereof of desinsection.
Present disclosure includes：
A kind of desinsection biological compound fungi agent, by weight, including compound bacteria 0.3-0.5%, strain protective agent 0.1-
0.5% and carrier 99.0-99.6%, the compound bacteria is thuringiensis, Paecilomyces lilacinus, beauveria bassiana, glimmering
Light Pseudomonas, streptomyces fradiae, bacillusmusilaginosiengineering, bacillus licheniformis and Streptomyces hygrospinosus.
In the present invention, as a kind of perferred technical scheme, by weight, the thuringiensis: pale purple quasi- blueness
Mould: beauveria bassiana: pseudomonas fluorescens: streptomyces fradiae: bacillusmusilaginosiengineering: bacillus licheniformis: thorn spore is inhaled
Water streptomycete=0.8: 1.2: 1.0: 1.25: 0.8: 0.8: 1.2: 1.0.
In the present invention, as a kind of perferred technical scheme, the strain protective agent is water-soluble sugar, activated carbon and degreasing
Milk powder, by weight, the water-soluble sugar: activated carbon: skimmed milk power=2: 1: 5.
In the present invention, as a kind of perferred technical scheme, the water-soluble sugar is fructose, glucose, lactose or sucrose
One or more of.
In the present invention, as a kind of perferred technical scheme, the carrier is cornstarch, diatomite and turf, by weight
Measure ratio, the cornstarch: diatomite: turf=1: 2: 2.
In inventive formulation：
Thuringiensis is a kind of production crystal bacillus, can be used for preventing Orthoptera, coleoptera, Diptera, film
The various pests of wing mesh, especially Lepidoptera.
Paecilomyces lilacinus belongs to endoparasitism fungi, is the Important Natural Enemy of some plant nematodes, can parasitize
Ovum can also infect larva and female adult, can the nemas such as substantially reduced various crop root-knot nematode, cyst nematode, Ditylenchus dipsaci
Harm；Also there is good fungistatic effect to various agricultural pathogenic bacteria.
Beauveria bassiana belongs to Neurospora section, can efficiently control population number, at the same do not injure other natural enemy insects and
Beneficial organism especially has good biological control effect to corn borer and pine moth.
Pseudomonas fluorescens have good promoting root growth growth result, and can prevent and inhibit tomato, eggplant, capsicum, cigarette
The generation of the soil-borne diseases such as bacterial wilt, the bacterial wilt of ginger of the solanaceous crops such as grass, and with the sprouting of promotion seed, raising germinating energy and go out
The advantages that seedling rate.
Streptomyces fradiae is the producing strains of tylosin, aerobic, is used in combination with bacillusmusilaginosiengineering, is had good
It is disease-resistant, provide metabolite and fixed nitrogen, phosphorus decomposing, potassium decomposing effect.
Bacillusmusilaginosiengineering is commonly called as B. mucilaginocus, has a utilization and capacity of decomposition to phosphorus potassium mineral, also have nitrogen fixing capacity and
It stimulates plant growth and inhibits the effect of phytopathogen.
Bacillus licheniformis can promote body to generate antibacterial substance, kill pathogenic bacteria.Resistant activity material can be generated,
And there is unique biology take oxygen mechanism, the growth and breeding of pathogenic bacteria can be inhibited.
Streptomyces hygrospinosus generates antifungal antibiotic, to bluish dogbane anthracnose, sweet potato black rot, canker of apple fruit etc.
Plant disease has control effect.
A kind of desinsection preparation method of biological compound fungi agent, includes the following steps：
(1) above-mentioned each strain is seeded in activation medium respectively under the conditions of 30~35 DEG C, 150~200r/min,
48h is cultivated, the seed liquor of each strain is obtained；
(2) seed liquor of each strain is inoculated into fermentation training respectively under the conditions of 30~35 DEG C, 150~200r/min
It supports in base, culture diffusion 48h obtains the zymotic fluid of each strain；
(3) zymotic fluid of each strain is mixed, adds after carrier is adsorbed and carries out freeze-day with constant temperature, ground after dry
It clays into power, obtains composite bacterium powder；
(4) composite bacterium powder is uniformly mixed with strain protective agent to get.
In the present invention, as a kind of perferred technical scheme, the activation medium is that mass percent is 10: 3: 5: 15
: 1000 peptone, beef extract, sodium chloride, agar and sterile water.
In the present invention, as a kind of perferred technical scheme, it is 200: 8: 5 that the fermentation medium, which is mass percent:
400: 100: 10: 8: 4: 2000 peptone, beef extract, magnesium chloride, soluble starch, sucrose, calcium carbonate, manganese sulfate, phosphorus
Acid dihydride potassium and sterile water.
In the present invention, as a kind of perferred technical scheme, the PH=7.0-7.2 of the fermentation medium.
In the present invention, as a kind of perferred technical scheme, the temperature of the freeze-day with constant temperature is 30~35 DEG C.
The beneficial effects of the invention are as follows：
Desinsection provided by the invention biological compound fungi agent, each microorganism fungus kind are built into dominant microflora, and insecticidal range is wide,
Can effectively prevent Orthoptera, coleoptera, Diptera, Hymenoptera, corn borer, pine moth, root-knot nematode, cyst nematode, Ditylenchus dipsaci,
Small sugarcane borer, blind Chinese toon, red spider and aphid etc., dosage is few, at low cost, can effectively inhibit the breeding of pernicious bacteria and pest,
The insect resistance capacity of plant can be improved, can also enhance soil activation, anti-adversity and soil fertility, improve Absorption and Utilization of Fertilizer rate,
The survival rate of higher efficient bacterium can be also kept while keeping higher killing rate, extend the time-to-live of efficient bacterium.
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment 1
A kind of desinsection biological compound fungi agent, by weight, including compound bacteria 0.3%, strain protective agent 0.1% and carrier
99.6%, compound bacteria is thuringiensis, Paecilomyces lilacinus, beauveria bassiana, pseudomonas fluorescens, Freund strepto-
Bacterium, bacillusmusilaginosiengineering, bacillus licheniformis and Streptomyces hygrospinosus.
Wherein, thuringiensis: Paecilomyces lilacinus: beauveria bassiana: pseudomonas fluorescens: streptomyces fradiae
: bacillusmusilaginosiengineering: bacillus licheniformis: Streptomyces hygrospinosus=0.8: 1.2: 1.0: 1.25: 0.8: 0.8: 1.2:
1.0；Strain protective agent is glucose, activated carbon and skimmed milk power, by weight, glucose: activated carbon: skimmed milk power=2: 1:
5。
Its preparation process is as follows：
(1) above-mentioned each strain is seeded in activation medium respectively under the conditions of 30 DEG C, 200r/min, cultivates 48h, obtains
The seed liquor of each strain；
(2) seed liquor of each strain is inoculated into fermentation medium respectively under the conditions of 30 DEG C, 200r/min, is trained
Diffusion 48h is supported, the zymotic fluid of each strain is obtained；
(3) zymotic fluid of each strain is mixed, adds after carrier adsorbed, freeze-day with constant temperature is carried out in 30 DEG C,
Grind into powder after drying, obtains composite bacterium powder；
(4) composite bacterium powder is uniformly mixed with strain protective agent to get.
Wherein, activation medium be mass percent be 10: 3: 5: 15: 1000 peptone, beef extract, sodium chloride,
Agar and sterile water, fermentation medium be mass percent be 200: 8: 5: 400: 100: 10: 8: 4: 2000 peptone, beef
Medicinal extract, magnesium chloride, soluble starch, sucrose, calcium carbonate, manganese sulfate, potassium dihydrogen phosphate and sterile water.
Embodiment 2
A kind of desinsection biological compound fungi agent, by weight, including compound bacteria 0.4%, strain protective agent 0.3% and carrier
99.3%, compound bacteria is thuringiensis, Paecilomyces lilacinus, beauveria bassiana, pseudomonas fluorescens, Freund strepto-
Bacterium, bacillusmusilaginosiengineering, bacillus licheniformis and Streptomyces hygrospinosus.
Wherein, thuringiensis: Paecilomyces lilacinus: beauveria bassiana: pseudomonas fluorescens: streptomyces fradiae
: bacillusmusilaginosiengineering: bacillus licheniformis: Streptomyces hygrospinosus=0.8: 1.2: 1.0: 1.25: 0.8: 0.8: 1.2:
1.0；Strain protective agent is glucose, activated carbon and skimmed milk power, by weight, sucrose: activated carbon: skimmed milk power=2: 1: 5.
Its preparation process is as follows：
(1) above-mentioned each strain is seeded in activation medium respectively under the conditions of 33 DEG C, 180r/min, cultivates 48h, obtains
The seed liquor of each strain；
(2) seed liquor of each strain is inoculated into fermentation training respectively under the conditions of 33 DEG C, 180r/min, PH=7.1
It supports in base, culture diffusion 48h obtains the zymotic fluid of each strain；
(3) it will add after carrier adsorbed, it is dry to carry out constant temperature in 33 DEG C after the zymotic fluid of each strain mixes
Dry, grind into powder after drying obtains composite bacterium powder；
(4) composite bacterium powder is uniformly mixed with strain protective agent to get.
Wherein, activation medium be mass percent be 10: 3: 5: 15: 1000 peptone, beef extract, sodium chloride,
Agar and sterile water, fermentation medium be mass percent be 200: 8: 5: 400: 100: 10: 8: 4: 2000 peptone, beef
Medicinal extract, magnesium chloride, soluble starch, sucrose, calcium carbonate, manganese sulfate, potassium dihydrogen phosphate and sterile water.
Embodiment 3
A kind of desinsection biological compound fungi agent, by weight, including compound bacteria 0.5%, strain protective agent 0.5% and carrier
99.0%, compound bacteria is thuringiensis, Paecilomyces lilacinus, beauveria bassiana, pseudomonas fluorescens, Freund strepto-
Bacterium, bacillusmusilaginosiengineering, bacillus licheniformis and Streptomyces hygrospinosus.
Wherein, thuringiensis: Paecilomyces lilacinus: beauveria bassiana: pseudomonas fluorescens: streptomyces fradiae
: bacillusmusilaginosiengineering: bacillus licheniformis: Streptomyces hygrospinosus=0.8: 1.2: 1.0: 1.25: 0.8: 0.8: 1.2:
1.0；Strain protective agent is glucose, activated carbon and skimmed milk power, by weight, lactose: activated carbon: skimmed milk power=2: 1: 5.
Its preparation process is as follows：
(1) above-mentioned each strain is seeded in activation medium respectively under the conditions of 35 DEG C, 150r/min, cultivates 48h, obtains
The seed liquor of each strain；
(2) seed liquor of each strain is inoculated into fermentation training respectively under the conditions of 35 DEG C, 150r/min, PH=7.2
It supports in base, culture diffusion 48h obtains the zymotic fluid of each strain；
(3) it will add after carrier adsorbed, it is dry to carry out constant temperature in 35 DEG C after the zymotic fluid of each strain mixes
Dry, grind into powder after drying obtains composite bacterium powder；
(4) composite bacterium powder is uniformly mixed with strain protective agent to get.
Wherein, activation medium be mass percent be 10: 3: 5: 15: 1000 peptone, beef extract, sodium chloride,
Agar and sterile water, fermentation medium be mass percent be 200: 8: 5: 400: 100: 10: 8: 4: 2000 peptone, beef
Medicinal extract, magnesium chloride, soluble starch, sucrose, calcium carbonate, manganese sulfate, potassium dihydrogen phosphate and sterile water.
Desinsection produced by the present invention is tested with biological compound fungi agent in Chinese cabbage field, with the vegetables in comparative example
Insecticide (active ingredient：Effective cypermethrin 4.5%), test effect comparison is carried out, test result see the table below 1：
| Nematode killing rate (%) | |
| Embodiment 1 | 91 |
| Embodiment 2 | 90 |
| Embodiment 3 | 92 |
| Comparative example | 87 |
Table 1
From table 1 it follows that desinsection provided by the invention is with biological compound fungi agent, the effect compared with comparative example differs not
Greatly, the present invention alternative existing chemical bactericide completely, and the present invention does not poison, it is pollution-free.
100 mu of milpas are randomly selected, the size of corn is tri-leaf period, and random division is experimental group 1, experimental group 2, experiment
Group 3, control group, every group 25 mu, it is ensured that 4 groups of situations are almost the same, and experimental group 1- experimental groups 3 use embodiment 1- embodiments respectively
The desinsection biological compound fungi agent of 3 gained, control group use common corn borer pesticide, it is ensured that fertilising, weeding, filling to corn
It is consistent other situations such as to irrigate, loosen the soil, the yield of every group of corn see the table below 2：
| Corn yield (kg/ mus) | |
| Experimental group 1 | 1286 |
| Experimental group 2 | 1305 |
| Experimental group 3 | 1298 |
| Control group | 1165 |
Table 2
From Table 2, it can be seen that desinsection provided by the invention can effectively kill corn borer with biological compound fungi agent, improve beautiful
Rice yield.
Desinsection produced by the present invention is applied in the soil with biological compound fungi agent in Planting Crops, can also be killed
Worm's ovum in soil prevents the pest and disease damage of plant root, improves crop yield.
Those of ordinary skills in the art should understand that：The discussion of any of the above embodiment is exemplary only, not
It is intended to imply that the scope of the present disclosure (including claim) is limited to these examples；Under the thinking of the present invention, above example
Or can also be combined between the technical characteristic in different embodiments, step can be realized with random order, and be existed such as
Many other variations of the different aspect of the upper present invention, for simplicity, they are not provided in details.Therefore, it is all
Within the spirit and principles in the present invention, any omission, modification, equivalent replacement, improvement for being made etc. should be included in the present invention's
Within protection domain.
Claims (10)
1. a kind of biological compound fungi agent of desinsection, characterized in that by weight, including compound bacteria 0.3-0.5%, strain protective agent
0.1-0.5% and carrier 99.0-99.6%, the compound bacteria are that thuringiensis, Paecilomyces lilacinus, ball spore are stiff in vain
Bacterium, pseudomonas fluorescens, streptomyces fradiae, bacillusmusilaginosiengineering, bacillus licheniformis and Streptomyces hygrospinosus.
2. desinsection as described in claim 1 biological compound fungi agent, characterized in that by weight, Su Yun gold brood cell's bar
Bacterium: Paecilomyces lilacinus: beauveria bassiana: pseudomonas fluorescens: streptomyces fradiae: bacillusmusilaginosiengineering: lichens gemma
Bacillus: Streptomyces hygrospinosus=0.8: 1.2: 1.0: 1.25: 0.8: 0.8: 1.2: 1.0.
3. desinsection as described in claim 1 biological compound fungi agent, characterized in that the strain protective agent be water-soluble sugar,
Activated carbon and skimmed milk power, by weight, the water-soluble sugar: activated carbon: skimmed milk power=2: 1: 5.
4. desinsection as claimed in claim 3 biological compound fungi agent, characterized in that the water-soluble sugar be fructose, glucose,
One or more of lactose or sucrose.
5. desinsection as described in claim 1 biological compound fungi agent, characterized in that the carrier is cornstarch, diatomite
And turf, by weight, the cornstarch: diatomite: turf=1: 2: 2.
6. a kind of desinsection preparation method of biological compound fungi agent, characterized in that include the following steps：
(1) above-mentioned each strain is seeded in activation medium respectively under the conditions of 30~35 DEG C, 150~200r/min, is cultivated
48h obtains the seed liquor of each strain；
(2) seed liquor of each strain is inoculated into fermentation medium respectively under the conditions of 30~35 DEG C, 150~200r/min
In, culture diffusion 48h obtains the zymotic fluid of each strain；
(3) zymotic fluid of each strain is mixed, adds after carrier is adsorbed and carries out freeze-day with constant temperature, be ground into after dry
Powder obtains composite bacterium powder；
(4) composite bacterium powder is uniformly mixed with strain protective agent to get.
7. the desinsection as claimed in claim 6 preparation method of biological compound fungi agent, characterized in that the activation medium is
Peptone, beef extract, sodium chloride, agar and the sterile water that mass percent is 10: 3: 5: 15: 1000.
8. the desinsection as claimed in claim 6 preparation method of biological compound fungi agent, characterized in that the fermentation medium is
Peptone that mass percent is 200: 8: 5: 400: 100: 10: 8: 4: 2000, beef extract, magnesium chloride, soluble starch, sugarcane
Sugar, calcium carbonate, manganese sulfate, potassium dihydrogen phosphate and sterile water.
9. the desinsection as claimed in claim 6 preparation method of biological compound fungi agent, characterized in that the fermentation medium
PH=7.0-7.2.
10. the desinsection as claimed in claim 6 preparation method of biological compound fungi agent, characterized in that the freeze-day with constant temperature
Temperature is 30~35 DEG C.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108395349A (en) * | 2017-02-08 | 2018-08-14 | 山东中创亿丰肥料集团有限公司 | A kind of composite biological fertilizer and preparation method thereof |
| CN109169710A (en) * | 2018-10-08 | 2019-01-11 | 山西农业大学 | A kind of prevention and treatment meloidogyne biological anti-bacteria agent and its application |
| CN111887246A (en) * | 2019-05-06 | 2020-11-06 | 成都特普生物科技股份有限公司 | Molecular chaperone for activating microbial agent |
| CN111903714A (en) * | 2019-05-10 | 2020-11-10 | 漯河市盛粮生物科技有限公司 | Preparation method of microbial agent for preventing and treating wheat root nematodes |
| CN112852671A (en) * | 2021-02-04 | 2021-05-28 | 兴安盟莱绅生物农业有限公司 | Microbial preparation for promoting corn growth |
| CN113453556A (en) * | 2018-12-11 | 2021-09-28 | 丹斯塔发酵股份公司 | Method for enhancing growth and survival of microorganisms |
| CN114456811A (en) * | 2021-12-01 | 2022-05-10 | 河北天善生物技术有限公司 | Farmland carbon-based soil remediation agent and preparation method thereof |
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| CN109169710A (en) * | 2018-10-08 | 2019-01-11 | 山西农业大学 | A kind of prevention and treatment meloidogyne biological anti-bacteria agent and its application |
| CN109169710B (en) * | 2018-10-08 | 2021-10-29 | 山西农业大学 | Biocontrol microbial inoculum for preventing and treating root-knot nematodes and application thereof |
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Application publication date: 20180814 |