CN109169710A - A kind of prevention and treatment meloidogyne biological anti-bacteria agent and its application - Google Patents
A kind of prevention and treatment meloidogyne biological anti-bacteria agent and its application Download PDFInfo
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Abstract
The invention belongs to microbial pesticide technical field, plant is single according to nematode single bacterial strain diseases prevention mechanism, control efficiency is relatively low in order to solve the problems, such as to prevent and treat at present, provides a kind of prevention and treatment meloidogyne biological anti-bacteria agent and its application.It is mixed by bacillus licheniformis-Pseudomonas fluorescens mixed fermentation liquid, streptomyces fradiae fermentation liquid, Phanerochaete chrysosporium-myrothecium verrucaria mixed fermentation liquid according to the volume ratio of 2-5:1-3:1-7, then mixed liquor is uniformly mixed for 1kg:6-8kg according to mass ratio with quinoa skin and is prepared.Five kinds of bacterial strains mutually not antagonism, mutual Synergistic insecticidal, and preparation process is relatively easy, has a extensive future.The present invention prevents and treats root knot nematode disease, promotes plant growth and development, improves disease resistance of plant.
Description
Technical field
The invention belongs to microbial pesticide technical fields, and in particular to a kind of prevention and treatment meloidogyne biological anti-bacteria agent and its answer
With.
Background technique
Root-knot nematode (Meloidogyne spp.) disease is the weight that Protected Production of Vegetables yield is seriously affected in global range
One of root disease is wanted, has the characteristics that widely distributed property, more host's property, strong infectivity and occurrence injury are hidden.With facility
The disease incidence of the fast development of vegetable growing, root-knot nematode rises year by year, serious area can underproduction 30-40%, or even total crop failure.Mesh
Before, agriculturally most important means of prevention is still pesticide and soil disinfection, causes pesticide largely to remain, environmental pollution.Therefore, it mentions
The quality and yield of high vegetables, seeks safe and efficient, environmentally friendly root knot nematode control measure and has become vegetables industry to compel
The important need of the eyebrows and eyelashes.Have now been found that, desk research show Pseudomonas fluorescens metabolite, myrothecium verrucaria conidium and
Metabolin, Phanerochaete chrysosporium conidium have very strong kill and parasitization to root-knot nematode J2, ovum, are more effectively
Prevention and treatment root-knot nematode biological and ecological methods to prevent plant disease, pests, and erosion strain, field experiment the result shows that, any one is difficult in these three biocontrol microorganisms of single administration
Root knot nematode disease is effectively prevented, activity is relatively low.
It is more using single microorganism prevention and treatment plant root-knot nematode report in biocontrol microorganisms application, but single bacterium
Strain diseases prevention mechanism is single, and definite value ability is poor, influences vulnerable to soil environment various factors, causes control efficiency relatively low.Pass through biological and ecological methods to prevent plant disease, pests, and erosion
Mutual affine synergistic effect between bacterium, realizes the mutual supplement with each other's advantages of more bacterial strains, can improve plant growth vigor itself or yield,
The Population of meloidogyne is reduced, increases the free nematode of soil, quantity of useful microbe, inhibits root-knot nematode to plant
Root is infected, to improve biocontrol effect and stability.
Summary of the invention
Plant is single according to nematode single bacterial strain diseases prevention mechanism, control efficiency is relatively low in order to solve to prevent and treat at present by the present invention
Problem provides a kind of prevention and treatment meloidogyne biological anti-bacteria agent and its application.
The present invention is realized by following technical solution: a kind of prevention and treatment meloidogyne biological anti-bacteria agent, by bacillus licheniformis
(Bacillus licheniformis)-Pseudomonas fluorescens (Pseudomonas fluorescens) mixed fermentation liquid, Freund
Streptomycete (Streptomyces fradiae) fermentation liquid, Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall)-myrothecium verrucaria (Myrothecium verrucaria) mixed fermentation liquid according to 2-5:1-3:1-7 volume ratio
Mixing, then mixed liquor is uniformly mixed for 1kg:6-8kg according to mass ratio with quinoa skin and is prepared；
Wherein: bacillus licheniformis (Bacillus licheniformis)-Pseudomonas fluorescens (Pseudomonas fluorescens) mixed fermentation liquid are as follows: bacillus licheniformis seed liquor and Pseudomonas fluorescens seed liquor press the volume of 1-2:1
Than mixing, after stirring 3-5 minutes, according in 3-8% volume ratio inoculum concentration access fermentation medium, 30 DEG C of cultivation 18-22h are obtained
Bacillus licheniformis-Pseudomonas fluorescens mixed fermentation liquid；
Streptomyces fradiae (Streptomyces fradiae) fermentation liquid are as follows: streptomyces fradiae seed liquor connects according to 3-8% volume ratio
In kind amount access fermentation medium, 29 DEG C of culture 7d obtain streptomyces fradiae fermentation liquid；
Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall)-myrothecium verrucaria
(Myrothecium verrucaria) mixed fermentation liquid are as follows: Phanerochaete chrysosporium seed liquor and myrothecium verrucaria seed liquor are pressed
The volume ratio of 1:1 mixes, and after stirring 3-5 minutes, according in 3-6% volume ratio inoculum concentration access fermentation medium, 28 DEG C are cultivated
5d obtains Phanerochaete chrysosporium-myrothecium verrucaria mixed fermentation liquid.
In the bacillus licheniformis-Pseudomonas fluorescens mixed fermentation liquid: the living bacteria count of bacillus licheniformis is
2.2-3.0×1010cfu•L−1 , Pseudomonas fluorescens living bacteria count be 1.0-1.6 × 1010cfu·L−1；Streptomyces fradiae
The living bacteria count of streptomyces fradiae is 1.4-3.3 × 10 in fermentation liquid6cfu·L−1；Phanerochaete chrysosporium-myrothecium verrucaria
In mixed fermentation liquid: the living bacteria count of Phanerochaete chrysosporium is 0.8-2.5 × 108cfu·L−1, myrothecium verrucaria it is effective
Viable count is 2.6-5.0 × 108cfu·L−1；Effective total viable count is 3.12-5.50 × 10 in the mixed liquor9cfu·L−1。
The bacillus licheniformis (Bacillus licheniformis) it is ACCC11080；Pseudomonas fluorescens
(Pseudomonas fluorescens) it is ACCC10646；Streptomyces fradiae (Streptomyces fradiae) be
ACCC40124；Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall) it is ACCC30530；Wart
Spore Myrothecum (Myrothecium verrucaria) it is ACCC30197.
35 DEG C of dry 48h, 200 mesh crush as prevention and treatment root-knot nematode biological and ecological methods to prevent plant disease, pests, and erosion after mixing for the mixed liquor and quinoa skin
Microbial inoculum.
It is preferred that: the bacillus licheniformis (Bacillus licheniformis)-Pseudomonas fluorescens
(Pseudomonas fluorescens) mixed fermentation liquid are as follows: bacillus licheniformis seed liquor and Pseudomonas fluorescens seed liquor
By 3-5min is stirred after the volume ratio mixing of 2:1, accessed in fermentation medium by the inoculum concentration of 6% volume ratio, 30 DEG C of cultivation 18-
22h is obtained；
The streptomyces fradiae (Streptomyces fradiae) fermentation liquid are as follows: streptomyces fradiae seed liquor is according to 8% volume ratio
Inoculum concentration accesses in fermentation medium, and 29 DEG C of culture 7d obtain streptomyces fradiae fermentation liquid；
The Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall)-myrothecium verrucaria
(Myrothecium verrucaria) mixed fermentation liquid are as follows: Phanerochaete chrysosporium seed liquor and myrothecium verrucaria seed liquor are pressed
The volume ratio of 1:1 mixes, and after stirring 3-5 minutes, accesses in fermentation medium according to 5% volume ratio inoculum concentration, 28 DEG C of culture 5d,
Obtain Phanerochaete chrysosporium-myrothecium verrucaria mixed fermentation liquid.
The bacillus licheniformis (Bacillus licheniformis)-Pseudomonas fluorescens (Pseudomonas fluorescens) fermentation medium that uses in mixed fermentation liquid are as follows: 15 gL of cornstarch−1, molasses 10gL−1, egg
White 1 gL of peptone−1, 2 gL of glucose−1, 5 gL of ammonium sulfate−1, KH2PO4 0.45 g·L−1, MgSO4 0.45 g·L−1；
The streptomyces fradiae (Streptomyces fradiae) fermentation medium that uses in fermentation liquid are as follows: glucose
6.0g·L−1, 6.0 gL of soybean cake powder−1, 35 gL of soluble starch−1, 2 gL of magnesium sulfate−1, 2 gL of calcium carbonate−1；
The Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall)-myrothecium verrucaria
(Myrothecium verrucaria) fermentation medium that uses in mixed fermentation liquid are as follows: 200 gL of potato−1, it is soluble
3 gL of starch−1, 0.8 gL of sodium nitrate−1, 15 gL of lactose−1, 5 gL of beef extract−1, 3 gL of ammonium sulfate−1。
Method of administration of the prepared biocontrol agent in field nematicidal is applied for cave, around every plant of root system of vegetable at protected field
8-12g is applied in cave, per acre 12-25 kilograms of dosage.
It should be noted that seed culture of the present invention and fermentation tank culture mode are this field routine techniques, and unknown
It states.
The present invention selects five kinds of bacterial strains from a variety of biocontrol microorganisms, in which: Mycophyta Phanerochaete chrysosporium and wart spore paint spot
Bacterium mainly kills root-knot nematode by conidium egg parasitoid and J2 mode；Bacterium class bacillus licheniformis and Pseudomonas fluorescens
Metabolite kills root-knot nematode J2, while promoting plant root system development, improves disease resistance of plant；Actinomyces streptomyces fradiae generation
It thanks to product and kills root-knot nematode J2, while promoting soil fungi quantity, cause the competition for carrying out nutrition and space with root-knot nematode.
This five kinds of bacterial strains mutually not antagonism, mutual Synergistic insecticidal, and preparation process is relatively easy, has a extensive future.The present invention prevents and treats vegetable
Dish root knot nematode disease promotes plant growth and development, improves disease resistance of plant.
Detailed description of the invention
Fig. 1 is prevention and treatment meloidogyne biological anti-bacteria agent prepared by the present invention to muskmelon root-knot nematode field control effect, figure
In: a-b: control, the muskmelon root system that do not prevent and treat；C-d: processing, the muskmelon root system after prevention and treatment.
Specific embodiment
It is specific below in conjunction with the application in order to make those skilled in the art more fully understand the technical solution in the application
Embodiment, carries out the technical solution of the application clear, is fully described by, it is clear that described embodiment is only the application one
Section Example, instead of all the embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not doing
Every other embodiment obtained under the premise of creative work out, shall fall within the protection scope of the present invention.
Embodiment 1: a kind of prevention and treatment meloidogyne biological anti-bacteria agent, by bacillus licheniformis (Bacillus licheniformis)-Pseudomonas fluorescens (Pseudomonas fluorescens) mixed fermentation liquid, streptomyces fradiae
(Streptomyces fradiae) fermentation liquid, Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall)-myrothecium verrucaria (Myrothecium verrucaria) mixed fermentation liquid according to 2:2:6 volume ratio mix,
Stirring after ten minutes, obtains liquid bacterial agent；Liquid bacterial agent and quinoa skin according to 1kg:6kg 35 DEG C of dry 48h after mixing,
It is prevention and treatment meloidogyne biological anti-bacteria agent that 200 mesh, which crush,.
Wherein: bacillus licheniformis (Bacillus licheniformis)-Pseudomonas fluorescens (Pseudomonas fluorescens) mixed fermentation liquid are as follows: bacillus licheniformis seed liquor and Pseudomonas fluorescens seed liquor press the volume ratio of 2:1
Mixing, after stirring 3-5 minutes, according in 6% volume ratio inoculum concentration access fermentation medium, 30 DEG C of cultivation 18-22h obtain lichens
Bacillus-Pseudomonas fluorescens mixed fermentation liquid；Fermentation medium are as follows: 15 gL of cornstarch−1, molasses 10gL−1,
1 gL of peptone−1, 2 gL of glucose−1, 5 gL of ammonium sulfate−1, KH2PO4 0.45 g·L−1, MgSO4 0.45 g·L−1；
Streptomyces fradiae (Streptomyces fradiae) fermentation liquid are as follows: streptomyces fradiae seed liquor presses 8% volume ratio inoculum concentration
It accesses in fermentation medium, 29 DEG C of culture 7d obtain streptomyces fradiae fermentation liquid；Fermentation medium are as follows: glucose 6.0gL−1, 6.0 gL of soybean cake powder−1, 35 gL of soluble starch−1, 2 gL of magnesium sulfate−1, 2 gL of calcium carbonate−1；
Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall)-myrothecium verrucaria
(Myrothecium verrucaria) mixed fermentation liquid are as follows: Phanerochaete chrysosporium seed liquor and myrothecium verrucaria seed liquor are pressed
The volume ratio of 1:1 mixes, and after stirring 3-5 minutes, accesses in fermentation medium according to 5% volume ratio inoculum concentration, 28 DEG C of culture 5d,
Obtain Phanerochaete chrysosporium-myrothecium verrucaria mixed fermentation liquid.Fermentation medium are as follows: 200 gL of potato−1, soluble form sediment
3 gL of powder−1, 0.8 gL of sodium nitrate−1, 15 gL of lactose−1, 5 gL of beef extract−1, 3 gL of ammonium sulfate−1。
In the bacillus licheniformis-Pseudomonas fluorescens mixed fermentation liquid: the living bacteria count of bacillus licheniformis is
2.2-3.0×1010cfu•L−1 , Pseudomonas fluorescens living bacteria count be 1.0-1.6 × 1010cfu·L−1；Streptomyces fradiae
The living bacteria count of streptomyces fradiae is 1.4-3.3 × 10 in fermentation liquid6cfu·L−1；Phanerochaete chrysosporium-myrothecium verrucaria
In mixed fermentation liquid: the living bacteria count of Phanerochaete chrysosporium is 0.8-2.5 × 108cfu·L−1, myrothecium verrucaria it is effective
Viable count is 2.6-5.0 × 108cfu·L−1；Effective total viable count is 3.12-5.50 × 10 in the mixed liquor9cfu·L−1。
The bacillus licheniformis (Bacillus licheniformis) it is ACCC11080；Pseudomonas fluorescens
(Pseudomonas fluorescens) it is ACCC10646；Streptomyces fradiae (Streptomyces fradiae) be
ACCC40124；Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall) it is ACCC30530；Wart
Spore Myrothecum (Myrothecium verrucaria) it is ACCC30197.
Method of administration of the prepared biocontrol agent in field nematicidal is applied for cave, around every plant of root system of vegetable at protected field
8g is applied in cave, per acre 12 kilograms of dosage.
Embodiment 2: a kind of prevention and treatment meloidogyne biological anti-bacteria agent, by bacillus licheniformis (Bacillus licheniformis)-Pseudomonas fluorescens (Pseudomonas fluorescens) mixed fermentation liquid, streptomyces fradiae
(Streptomyces fradiae) fermentation liquid, Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall)-myrothecium verrucaria (Myrothecium verrucaria) mixed fermentation liquid according to 3:1:7 volume ratio mix,
Stirring after ten minutes, obtains liquid bacterial agent；Liquid bacterial agent and quinoa skin according to 1kg:8kg 35 DEG C of dry 48h after mixing,
It is prevention and treatment meloidogyne biological anti-bacteria agent that 200 mesh, which crush,.
Wherein: bacillus licheniformis (Bacillus licheniformis)-Pseudomonas fluorescens (Pseudomonas fluorescens) mixed fermentation liquid are as follows: bacillus licheniformis seed liquor and Pseudomonas fluorescens seed liquor press the volume ratio of 1:1
Mixing, after stirring 3-5 minutes, according in 3% volume ratio inoculum concentration access fermentation medium, 30 DEG C of cultivation 18-22h obtain lichens
Bacillus-Pseudomonas fluorescens mixed fermentation liquid；Fermentation medium are as follows: 15 gL of cornstarch−1, molasses 10gL−1,
1 gL of peptone−1, 2 gL of glucose−1, 5 gL of ammonium sulfate−1, KH2PO4 0.45 g·L−1, MgSO4 0.45 g·L−1；
Streptomyces fradiae (Streptomyces fradiae) fermentation liquid are as follows: streptomyces fradiae seed liquor presses 5% volume ratio inoculum concentration
It accesses in fermentation medium, 29 DEG C of culture 7d obtain streptomyces fradiae fermentation liquid；Fermentation medium are as follows: glucose 6.0gL−1, 6.0 gL of soybean cake powder−1, 35 gL of soluble starch−1, 2 gL of magnesium sulfate−1, 2 gL of calcium carbonate−1；
Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall)-myrothecium verrucaria
(Myrothecium verrucaria) mixed fermentation liquid are as follows: Phanerochaete chrysosporium seed liquor and myrothecium verrucaria seed liquor are pressed
The volume ratio of 1:1 mixes, and after stirring 3-5 minutes, accesses in fermentation medium according to 3% volume ratio inoculum concentration, 28 DEG C of culture 5d,
Obtain Phanerochaete chrysosporium-myrothecium verrucaria mixed fermentation liquid.Fermentation medium are as follows: 200 gL of potato−1, soluble form sediment
3 gL of powder−1, 0.8 gL of sodium nitrate−1, 15 gL of lactose−1, 5 gL of beef extract−1, 3 gL of ammonium sulfate−1。
In the bacillus licheniformis-Pseudomonas fluorescens mixed fermentation liquid: the living bacteria count of bacillus licheniformis is
2.2-3.0×1010cfu•L−1 , Pseudomonas fluorescens living bacteria count be 1.0-1.6 × 1010cfu·L−1；Streptomyces fradiae
The living bacteria count of streptomyces fradiae is 1.4-3.3 × 10 in fermentation liquid6cfu·L−1；Phanerochaete chrysosporium-myrothecium verrucaria
In mixed fermentation liquid: the living bacteria count of Phanerochaete chrysosporium is 0.8-2.5 × 108cfu·L−1, myrothecium verrucaria it is effective
Viable count is 2.6-5.0 × 108cfu·L−1；Effective total viable count is 3.12-5.50 × 10 in the mixed liquor9cfu·L−1。
The bacillus licheniformis (Bacillus licheniformis) it is ACCC11080；Pseudomonas fluorescens
(Pseudomonas fluorescens) it is ACCC10646；Streptomyces fradiae (Streptomyces fradiae) be
ACCC40124；Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall) it is ACCC30530；Wart
Spore Myrothecum (Myrothecium verrucaria) it is ACCC30197.
Method of administration of the prepared biocontrol agent in field nematicidal is applied for cave, around every plant of root system of vegetable at protected field
10g is applied in cave, per acre 20 kilograms of dosage.
Embodiment 3: a kind of prevention and treatment meloidogyne biological anti-bacteria agent, by bacillus licheniformis (Bacillus licheniformis)-Pseudomonas fluorescens (Pseudomonas fluorescens) mixed fermentation liquid, streptomyces fradiae
(Streptomyces fradiae) fermentation liquid, Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall)-myrothecium verrucaria (Myrothecium verrucaria) mixed fermentation liquid according to 5:3:1 volume ratio mix,
Stirring after ten minutes, obtains liquid bacterial agent；Liquid bacterial agent and quinoa skin according to 1kg:7kg 35 DEG C of dry 48h after mixing,
It is prevention and treatment meloidogyne biological anti-bacteria agent that 200 mesh, which crush,.
Wherein: bacillus licheniformis (Bacillus licheniformis)-Pseudomonas fluorescens (Pseudomonas fluorescens) mixed fermentation liquid are as follows: bacillus licheniformis seed liquor and Pseudomonas fluorescens seed liquor press the volume of 1.5:1
Than mixing, after stirring 3-5 minutes, according in 8% volume ratio inoculum concentration access fermentation medium, 30 DEG C of cultivation 18-22h obtain ground
Clothing bacillus-Pseudomonas fluorescens mixed fermentation liquid；Fermentation medium are as follows: 15 gL of cornstarch−1, molasses 10gL−1, 1 gL of peptone−1, 2 gL of glucose−1, 5 gL of ammonium sulfate−1, KH2PO4 0.45 g·L−1, MgSO4 0.45
g·L−1；
Streptomyces fradiae (Streptomyces fradiae) fermentation liquid are as follows: streptomyces fradiae seed liquor presses 3% volume ratio inoculum concentration
It accesses in fermentation medium, 29 DEG C of culture 7d obtain streptomyces fradiae fermentation liquid；Fermentation medium are as follows: glucose 6.0gL−1, 6.0 gL of soybean cake powder−1, 35 gL of soluble starch−1, 2 gL of magnesium sulfate−1, 2 gL of calcium carbonate−1；
Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall)-myrothecium verrucaria
(Myrothecium verrucaria) mixed fermentation liquid are as follows: Phanerochaete chrysosporium seed liquor and myrothecium verrucaria seed liquor are pressed
The volume ratio of 1:1 mixes, and after stirring 3-5 minutes, accesses in fermentation medium according to 6% volume ratio inoculum concentration, 28 DEG C of culture 5d,
Obtain Phanerochaete chrysosporium-myrothecium verrucaria mixed fermentation liquid.Fermentation medium are as follows: 200 gL of potato−1, soluble form sediment
3 gL of powder−1, 0.8 gL of sodium nitrate−1, 15 gL of lactose−1, 5 gL of beef extract−1, 3 gL of ammonium sulfate−1。
In the bacillus licheniformis-Pseudomonas fluorescens mixed fermentation liquid: the living bacteria count of bacillus licheniformis is
2.2-3.0×1010cfu•L−1 , Pseudomonas fluorescens living bacteria count be 1.0-1.6 × 1010cfu·L−1；Streptomyces fradiae
The living bacteria count of streptomyces fradiae is 1.4-3.3 × 10 in fermentation liquid6cfu·L−1；Phanerochaete chrysosporium-myrothecium verrucaria
In mixed fermentation liquid: the living bacteria count of Phanerochaete chrysosporium is 0.8-2.5 × 108cfu·L−1, myrothecium verrucaria it is effective
Viable count is 2.6-5.0 × 108cfu·L−1；Effective total viable count is 3.12-5.50 × 10 in the mixed liquor9cfu·L−1。
The bacillus licheniformis (Bacillus licheniformis) it is ACCC11080；Pseudomonas fluorescens
(Pseudomonas fluorescens) it is ACCC10646；Streptomyces fradiae (Streptomyces fradiae) be
ACCC40124；Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall) it is ACCC30530；Wart
Spore Myrothecum (Myrothecium verrucaria) it is ACCC30197.
Method of administration of the prepared biocontrol agent in field nematicidal is applied for cave, around every plant of root system of vegetable at protected field
12g is applied in cave, per acre 25 kilograms of dosage.
Experimental example 1: field trial: experiment occurs serious vegetable field in Taigu Xiang Gu root-knot nematode and carries out.For studying object
For muskmelon, start to apply microbial inoculum before transplanting.12 grams of composite bacteria agents of every plant of dosage compare as equivalent quinoa skin.Picking time investigates root
Index is tied, control efficiency is evaluated.Root knot severity divides 0-10 grades, and control efficiency=(check plot disease index-treatment region state of an illness refers to
Number)/check plot disease index × 100.
Control efficiency is shown in Table 1, the results showed that says that composite bacteria agent can effectively prevent muskmelon root knot nematode disease, control efficiency is
53.7%, plant biological yield is significantly improved, treatment region plant increases 25.3%, and root fresh weight increases by 50.9%, as seen from Figure 1
Treatment region muskmelon well developed root system, experimental control area muskmelon root system are tiny, there was only a small amount of root knot on treatment region muskmelon.
1 composition of table is to muskmelon root-knot nematode field control effect
Claims (7)
1. a kind of prevention and treatment meloidogyne biological anti-bacteria agent, it is characterised in that: by bacillus licheniformis (Bacillus licheniformis)-Pseudomonas fluorescens (Pseudomonas fluorescens) mixed fermentation liquid, streptomyces fradiae
(Streptomyces fradiae) fermentation liquid, Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall)-myrothecium verrucaria (Myrothecium verrucaria) mixed fermentation liquid according to 2-5:1-3:1-7 volume ratio
Mixing, then mixed liquor is uniformly mixed for 1kg:6-8kg according to mass ratio with quinoa skin and is prepared；
Wherein: bacillus licheniformis (Bacillus licheniformis)-Pseudomonas fluorescens (Pseudomonas fluorescens) mixed fermentation liquid are as follows: bacillus licheniformis seed liquor and Pseudomonas fluorescens seed liquor press the volume of 1-2:1
Than mixing, after stirring 3-5 minutes, according in 3-8% volume ratio inoculum concentration access fermentation medium, 30 DEG C of cultivation 18-22h are obtained
Bacillus licheniformis-Pseudomonas fluorescens mixed fermentation liquid；
Streptomyces fradiae (Streptomyces fradiae) fermentation liquid are as follows: streptomyces fradiae seed liquor connects according to 3-8% volume ratio
In kind amount access fermentation medium, 29 DEG C of culture 7d obtain streptomyces fradiae fermentation liquid；
Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall)-myrothecium verrucaria
(Myrothecium verrucaria) mixed fermentation liquid are as follows: Phanerochaete chrysosporium seed liquor and myrothecium verrucaria seed liquor are pressed
The volume ratio of 1:1 mixes, and after stirring 3-5 minutes, according in 3-6% volume ratio inoculum concentration access fermentation medium, 28 DEG C are cultivated
5d obtains Phanerochaete chrysosporium-myrothecium verrucaria mixed fermentation liquid.
2. a kind of prevention and treatment meloidogyne biological anti-bacteria agent according to claim 1, it is characterised in that: the lichens gemma bar
In bacterium-Pseudomonas fluorescens mixed fermentation liquid: the living bacteria count of bacillus licheniformis is 2.2-3.0 × 1010cfu•L−1 , it is glimmering
The living bacteria count of light pseudomonad is 1.0-1.6 × 1010cfu·L−1；Streptomyces fradiae has in streptomyces fradiae fermentation liquid
Effect viable count is 1.4-3.3 × 106cfu·L−1；In Phanerochaete chrysosporium-myrothecium verrucaria mixed fermentation liquid: yellow archespore hair
The living bacteria count of flat lead fungi is 0.8-2.5 × 108cfu·L−1, myrothecium verrucaria living bacteria count be 2.6-5.0 ×
108cfu·L−1；Effective total viable count is 3.12-5.50 × 10 in the mixed liquor9cfu·L−1。
3. a kind of prevention and treatment meloidogyne biological anti-bacteria agent according to claim 1, it is characterised in that: in the fermentation liquid: institute
State bacillus licheniformis (Bacillus licheniformis) it is ACCC11080；Pseudomonas fluorescens (Pseudomonas fluorescens) it is ACCC10646；Streptomyces fradiae (Streptomyces fradiae) it is ACCC40124；Yellow archespore hair
Flat lead fungi (Phanerochaete chrysosporium Burdsall) it is ACCC30530；Myrothecium verrucaria
(Myrothecium verrucaria) it is ACCC30197.
4. a kind of prevention and treatment meloidogyne biological anti-bacteria agent according to claim 1, it is characterised in that: the mixed liquor and quinoa
35 DEG C of dry 48h, 200 mesh crush as prevention and treatment meloidogyne biological anti-bacteria agent to skin after mixing.
5. a kind of prevention and treatment meloidogyne biological anti-bacteria agent according to claim 1, it is characterised in that: the bacillus licheniformis
(Bacillus licheniformis)-Pseudomonas fluorescens (Pseudomonas fluorescens) mixed fermentation liquid are as follows: ground
Clothing bacillus seed liquor and Pseudomonas fluorescens seed liquor stir 3-5min after mixing by the volume ratio of 2:1, by 6% volume ratio
Inoculum concentration access fermentation medium in, 30 DEG C of cultivations 18-22h acquisitions；
The streptomyces fradiae (Streptomyces fradiae) fermentation liquid are as follows: streptomyces fradiae seed liquor is according to 8% volume ratio
Inoculum concentration accesses in fermentation medium, and 29 DEG C of culture 7d obtain streptomyces fradiae fermentation liquid；
The Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall)-myrothecium verrucaria
(Myrothecium verrucaria) mixed fermentation liquid are as follows: Phanerochaete chrysosporium seed liquor and myrothecium verrucaria seed liquor are pressed
The volume ratio of 1:1 mixes, and after stirring 3-5 minutes, accesses in fermentation medium according to 5% volume ratio inoculum concentration, 28 DEG C of culture 5d,
Obtain Phanerochaete chrysosporium-myrothecium verrucaria mixed fermentation liquid.
6. a kind of prevention and treatment meloidogyne biological anti-bacteria agent according to claim 1, it is characterised in that: the bacillus licheniformis
(Bacillus licheniformis)-Pseudomonas fluorescens (Pseudomonas fluorescens) adopt in mixed fermentation liquid
Fermentation medium are as follows: 15 gL of cornstarch−1, molasses 10gL−1, 1 gL of peptone−1, 2 gL of glucose−1, 5 gL of ammonium sulfate−1, KH2PO4 0.45 g·L−1, MgSO4 0.45 g·L−1；
The streptomyces fradiae (Streptomyces fradiae) fermentation medium that uses in fermentation liquid are as follows: glucose
6.0g·L−1, 6.0 gL of soybean cake powder−1, 35 gL of soluble starch−1, 2 gL of magnesium sulfate−1, 2 gL of calcium carbonate−1；
The Phanerochaete chrysosporium (Phanerochaete chrysosporium Burdsall)-myrothecium verrucaria
(Myrothecium verrucaria) fermentation medium that uses in mixed fermentation liquid are as follows: 200 gL of potato−1, it is soluble
3 gL of starch−1, 0.8 gL of sodium nitrate−1, 15 gL of lactose−1, 5 gL of beef extract−1, 3 gL of ammonium sulfate−1。
7. a kind of prevention and treatment meloidogyne biological anti-bacteria agent according to claim 1, it is characterised in that: prepared biocontrol agent
Method of administration in field when nematicidal is applied for cave, and 8-12g is applied in cave around every plant of root system of vegetable at protected field, per acre dosage 12-25
Kilogram.
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| CN113462599A (en) * | 2021-07-01 | 2021-10-01 | 云南大学 | Biological preparation containing plant source components and microorganism source components and application of biological preparation in prevention and treatment of plant nematode diseases |
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| CN108395998A (en) * | 2017-02-08 | 2018-08-14 | 山东中创亿丰肥料集团有限公司 | A kind of desinsection biological compound fungi agent and preparation method thereof |
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| CN108395998A (en) * | 2017-02-08 | 2018-08-14 | 山东中创亿丰肥料集团有限公司 | A kind of desinsection biological compound fungi agent and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113462599A (en) * | 2021-07-01 | 2021-10-01 | 云南大学 | Biological preparation containing plant source components and microorganism source components and application of biological preparation in prevention and treatment of plant nematode diseases |
| CN113462599B (en) * | 2021-07-01 | 2022-07-15 | 云南大学 | Biological preparation containing plant source components and microorganism source components and application of biological preparation in prevention and treatment of plant nematode diseases |
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