CN111887246A - Molecular chaperone for activating microbial agent - Google Patents
Molecular chaperone for activating microbial agent Download PDFInfo
- Publication number
- CN111887246A CN111887246A CN201910370321.3A CN201910370321A CN111887246A CN 111887246 A CN111887246 A CN 111887246A CN 201910370321 A CN201910370321 A CN 201910370321A CN 111887246 A CN111887246 A CN 111887246A
- Authority
- CN
- China
- Prior art keywords
- bacillus
- microbial
- starch
- microbial inoculum
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A molecular chaperone for activating microbial agents. The invention belongs to the technical field of biology, and relates to a method for activating a molecular chaperone of a microbial inoculant to activate the microbial inoculant with short shelf life after long-time storage, so that the microbial inoculant can quickly grow and propagate on a carrier to form a dominant strain, and the technical problem of long-time storage of the microbial inoculant, especially a pseudomonas fluorescens product with weak stress resistance, is solved.
Description
Technical Field
The present invention belongs to the field of biological pesticide and biochemical technology.
Background
Chemical pesticides play an important role in controlling agricultural pests, protecting agricultural production safety, reducing labor intensity and the like, but with the wide application of a large amount of chemical pesticides, environmental problems and video safety problems caused by chemical pesticide residues are increasingly concerned. The search for new agricultural measures meeting the agricultural sustainable development needs becomes a hot spot of the current agricultural production research. The biocontrol bacteria and metabolites thereof can not only inhibit the growth of various pathogenic bacteria, but also promote the growth of plants and improve the yield of crops, and the development of biocontrol strains and effective antibacterial active ingredients thereof become the focus of research and competition at present.
The fatal defects of microbial pesticides are sensitivity to external conditions under natural conditions, easy death and short shelf life. The problems of short shelf life, easy death in nature, easy influence by environment and the like of microbial pesticides can be solved by improving the dosage form. In order to prolong the storage period, many microbial pesticides are optimized through fermentation to culture organisms with strong stress resistance, such as spores, chlamydospores and the like, and then are prepared into wettable powder, micro-capsule granules, water dispersible granules and the like. The method increases the resistance of the microbial pesticide under natural conditions to a certain extent, but the shelf life of some microbial bacteria is still difficult to prolong because the microbial bacteria do not generate structures with stronger stress resistance such as spores or chlamydospores, for example, the shelf life of the pseudomonas fluorescens microbial inoculum widely used in the world is very short, and the national standard of the pseudomonas fluorescens shelf life established in our country is 6 months.
Disclosure of Invention
A molecular chaperone for activating microbial inoculum is characterized in that microbial strains are inoculated to a carrier material to prepare microbial inoculum, molecular chaperones containing carbohydrate or protein or amino acid or protease or nucleic acid or fatty acid or derivatives thereof are prepared to activate the microbial inoculum, and the molecular chaperones and the microbial inoculum are mixed uniformly before or during the use of the microbial inoculum for regulating the growth and development of plants, preventing and controlling plant diseases and insect pests and harmful algae in water.
The microorganism includes, but is not limited to, Bacillus subtilis, Pseudomonas fluorescens, Bacillus pumilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus cereus, Paenibacillus polymyxa, Bacillus psychrophilus, Streptomyces hygroscopicus, Trichoderma, Verticillium pachycem, Bacillus firmus, Bacillus thuringiensis, Beauveria bassiana, Paecilomyces lilacinus, and the like, and more preferably Pseudomonas fluorescens.
The carrier material is characterized by comprising but not limited to one or more of active carbon, diatomite, white carbon black, medical stone, kaolin, bran shell, carboxymethyl starch, hydroxyethyl starch, water, soluble starch, porous starch and macroporous adsorption starch, and the medical stone is more preferable.
The molecular chaperone for activating microbial inoculum is one or more of carbohydrate or protein or amino acid or protease or nucleic acid or fatty acid or their derivatives, which is characterized by comprising but not limited to one or more of shellac, milk powder, carboxymethyl starch, glyceryl monostearate, lysine, arginine, glutamic acid, methionine, S-adenosylmethionine, aspartic acid, glycine, methionine, histidine, polypeptide, peptone, gelatin, trehalose, sucrose, sorbose, melezitose, glycerol, fructose, mannose, maltose, lactose, lactic acid, calcium lactate, butyric acid, arabinose, xylose, ribose, rhamnose, glucose, mannitol, glycerol, xylitol, erythritol, threitol, sorbitol and raffinose, dextrin, dextran and soluble starch, more preferred is S-adenosylmethionine.
The plant diseases and insect pests include, but are not limited to, diseases caused by plant-harmful microorganisms such as nematodes, viruses, fungi or bacteria and insect pests caused by insects such as chewing mouthparts, piercing-sucking mouthparts, filing-sucking mouthparts, scraping-sucking mouthparts and feeding-sucking mouthparts. The harmful algae in the water body is characterized by comprising but not limited to blue algae, dinoflagellate and the like which pollute the water body.
The invention has the innovation point that the molecular chaperone for activating the microbial agent is creatively prepared, so that the microbial agent with short shelf life can be activated by the molecular chaperone after being stored for a long time, the microbial agent on a carrier material can quickly grow and propagate on the carrier to form a dominant strain, and the technical problem of long-term storage of the microbial agent, especially the pseudomonas fluorescens product with weak stress resistance, is solved.
The invention solves the technical problem that the biological pesticide can still stably exert the effect after being stored for a long time through the nutrition mate provided for the live bacteria biological pesticide.
Detailed description of the preferred embodiments
The present invention is further described in terms of the following examples, which are included to provide further understanding to those skilled in the art. Any modifications and alterations made without departing from the spirit of the invention are within the scope of the invention.
Embodiment one
1. Preparation of microbial agent
Inoculating pseudomonas fluorescens to an LB culture medium, culturing for 72 hours at the temperature of 28 ℃ and at the speed of 150 r/min, uniformly inoculating fermentation liquor to medical stone serving as an adsorbing material carrier to prepare pseudomonas fluorescens microbial inoculum, packaging the microbial inoculum in a sealed container, and storing at normal temperature. Sampling and determining the bacteria content.
Mixing 10 g of glycerol with 100ml of water, adding 1.3 g of S-adenosylmethionine, mixing uniformly to prepare the molecular chaperone for activating the microbial inoculum, packaging in a sealed container, and storing at normal temperature.
When the pseudomonas fluorescens microbial inoculum is stored for 1 week, 1 month, 3 months, 6 months, 12 months, 18 months and 24 months, 30 g of the microbial inoculum: 5.5 g of molecular chaperone, uniformly mixing the molecular chaperone and the pseudomonas fluorescens microbial inoculum, and measuring the content of the bacteria after standing for 24 hours at normal temperature. The results are shown in Table 1.
TABLE 1 Effect of molecular chaperones on Pseudomonas fluorescens content
| Time of detection | Microbial agent | Microbial agent + molecular chaperone |
| Initial detection of content | 5.2x109 | 5.2x109 |
| 1 week | 1.2x109 | 2.2x109 |
| 1 month | 3.5x108 | 4.3x109 |
| 3 months old | 1.5x107 | 7.8x109 |
| 6 months old | 4.5x106 | 1.3x109 |
| 12 months old | 7.1x106 | 1.2x109 |
| 18 months old | 5.1x105 | 2.3x109 |
| 24 months | 1.6x105 | 6.1x109 |
The result shows that the viable bacteria amount of the pseudomonas fluorescens microbial agent begins to be reduced sharply after being stored for 1 month at normal temperature, and the viable bacteria amount is reduced to the million level after 6 months. And the molecular chaperone is added, the pseudomonas fluorescens microbial agent still keeps relatively stable after being stored for 24 months at normal temperature, and the effect of activating the activity of the bacterial strain by the microbial agent chaperone is very obvious.
Claims (6)
1. A molecular chaperone for activating microbial inoculum is characterized in that microbial strains are inoculated to a carrier material to prepare microbial inoculum, molecular chaperones containing carbohydrate or protein or amino acid or protease or nucleic acid or fatty acid or derivatives thereof are prepared to activate the microbial inoculum, and the molecular chaperones and the microbial inoculum are mixed uniformly before or during the use of the microbial inoculum for regulating the growth and development of plants, preventing and controlling plant diseases and insect pests and harmful algae in water.
2. The microbial organism of claim 1, wherein: the microorganism includes, but is not limited to, Bacillus subtilis, Pseudomonas fluorescens, Bacillus pumilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus cereus, Paenibacillus polymyxa, Bacillus psychrophilus, Streptomyces hygroscopicus, Trichoderma, Verticillium pachycem, Bacillus firmus, Bacillus thuringiensis, Beauveria bassiana, Paecilomyces lilacinus, and the like, and more preferably Pseudomonas fluorescens.
3. The carrier material of claim 1 comprising, but not limited to, one or more of activated carbon, diatomaceous earth, white carbon, medical stone, kaolin, bran coat, carboxymethyl starch, hydroxyethyl starch, water, soluble starch, porous starch and macroporous adsorption starch, more preferably medical stone.
4. The chaperone for activating microbial agents according to claim 1, which is one or more of carbohydrates or proteins or amino acids or proteases or nucleic acids or fatty acids or their derivatives, including but not limited to shellac, milk powder, carboxymethyl starch, glyceryl monostearate, lysine, arginine, glutamic acid, methionine, S-adenosylmethionine, aspartic acid, glycine, methionine, histidine, polypeptides, peptone, gelatin, trehalose, sucrose, sorbose, melezitose, glycerol, fructose, mannose, maltose, lactose, lactic acid, calcium lactate, butyric acid, arabinose, xylose, ribose, rhamnose, glucose, mannitol, glycerol, xylitol, kakkol, threitol, sorbitol and raffinose, dextrin, dextran and starch soluble, more preferred is S-adenosylmethionine.
5. A plant pest according to claim 1 including, but not limited to, plant-damaging micro-organisms such as nematodes, viruses, fungi or bacteria infested to cause disease and insect pests from chewing mouthparts, piercing-sucking mouthparts, rasping-sucking mouthparts, scraping mouthparts and feeding mouthparts.
6. The harmful algae in water of claim 1, including but not limited to cyanobacteria, dinoflagellates, and the like that contaminate water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910370321.3A CN111887246A (en) | 2019-05-06 | 2019-05-06 | Molecular chaperone for activating microbial agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910370321.3A CN111887246A (en) | 2019-05-06 | 2019-05-06 | Molecular chaperone for activating microbial agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111887246A true CN111887246A (en) | 2020-11-06 |
Family
ID=73169361
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910370321.3A Withdrawn CN111887246A (en) | 2019-05-06 | 2019-05-06 | Molecular chaperone for activating microbial agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111887246A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979220A (en) * | 2019-05-24 | 2020-11-24 | 成都特普生物科技股份有限公司 | Carrier molecular chaperone for activating microbial preparation |
| CN113621637A (en) * | 2021-08-16 | 2021-11-09 | 广西科学院 | Escherichia coli solubilizing expression plasmid and construction method and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104247717A (en) * | 2013-06-25 | 2014-12-31 | 中国科学院沈阳应用生态研究所 | Microbial inoculant inhibiting diseases and insect pests of plants, and preparation method and application thereof |
| US20160073640A1 (en) * | 2014-09-17 | 2016-03-17 | Bayer Cropscience Lp | Compositions comprising recombinant bacillus cells and another biological control agent |
| CN105969699A (en) * | 2016-07-12 | 2016-09-28 | 佛山杰致信息科技有限公司 | Agricultural ecological probiotic composite microbial inoculant |
| CN108395998A (en) * | 2017-02-08 | 2018-08-14 | 山东中创亿丰肥料集团有限公司 | A kind of desinsection biological compound fungi agent and preparation method thereof |
-
2019
- 2019-05-06 CN CN201910370321.3A patent/CN111887246A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104247717A (en) * | 2013-06-25 | 2014-12-31 | 中国科学院沈阳应用生态研究所 | Microbial inoculant inhibiting diseases and insect pests of plants, and preparation method and application thereof |
| US20160073640A1 (en) * | 2014-09-17 | 2016-03-17 | Bayer Cropscience Lp | Compositions comprising recombinant bacillus cells and another biological control agent |
| CN105969699A (en) * | 2016-07-12 | 2016-09-28 | 佛山杰致信息科技有限公司 | Agricultural ecological probiotic composite microbial inoculant |
| CN108395998A (en) * | 2017-02-08 | 2018-08-14 | 山东中创亿丰肥料集团有限公司 | A kind of desinsection biological compound fungi agent and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 石门: "《微生物学》", 31 January 2005, 远方出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979220A (en) * | 2019-05-24 | 2020-11-24 | 成都特普生物科技股份有限公司 | Carrier molecular chaperone for activating microbial preparation |
| CN113621637A (en) * | 2021-08-16 | 2021-11-09 | 广西科学院 | Escherichia coli solubilizing expression plasmid and construction method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Jeong et al. | Characterization of a multifunctional feather-degrading Bacillus subtilis isolated from forest soil | |
| CN110272854B (en) | Bacillus subtilis strain and application thereof | |
| AU2015300988A1 (en) | Bacterial spore compositions for industrial uses | |
| CN105557756A (en) | Plant bacteriostasis method adopting bacillus methylotrophicus strain NKG-1 | |
| US20100119486A1 (en) | Antibacterial treatment method | |
| CN111979220A (en) | Carrier molecular chaperone for activating microbial preparation | |
| CN110295129B (en) | Biocontrol bacterium for preventing and treating gray mold and powdery mildew of cucumber and application thereof | |
| KR102176078B1 (en) | Solid culture method to culture with new microbial bacillus subtilis BS300 strains with resolution to carbohydrates and proteins and fibres among organic matter | |
| CN103966113A (en) | Composite bacterium inoculant for degrading residual pesticide and production method thereof | |
| CN111887246A (en) | Molecular chaperone for activating microbial agent | |
| CN107075459B (en) | Novel bacterium belonging to the genus Bacillus and use thereof | |
| CN109804061A (en) | Method for culturing spore-forming bacteria and method for producing useful substance | |
| KR20230043197A (en) | Materials and methods for improving plant health | |
| CN111533586B (en) | Chicken manure bio-organic fertilizer and preparation method thereof | |
| Artha et al. | Identification of extracellular enzyme-producing bacteria (proteolytic, cellulolytic, and amylolytic) in the sediment of extensive ponds in Tanggulrejo, Gresik | |
| Abada | Isolation and characterization of a antimicrobial compound from Bacillus coagulans | |
| KR20080045812A (en) | Method for enhancing the thermal tolerance of entomopathogenic fungal spores, blastospores and enzymes | |
| CN111280181A (en) | Powder moistening microbial inoculum for directional culture and preservation of microorganisms | |
| KR100475559B1 (en) | A microbes pesticide active against brevibacillus compost rot boil effect and process for preparation thereof | |
| CA3155789A1 (en) | Biological control agent formulations including trichoderma | |
| ES2084014T3 (en) | PESTICIDE COMPOSITIONS BASED ON MICROORGANISMS, THEIR PREPARATION PROCEDURE AND THEIR APPLICATION IN AGRONOMY. | |
| CN114369550A (en) | Bacillus amyloliquefaciens for promoting oat growth and application thereof | |
| RU2551968C2 (en) | Bacillus pumilus A 1.5 BACTERIA STRAIN AS AGENT FOR INCREASING PLANT PRODUCTIVITY AND PLANT PROTECTION FROM DISEASES CAUSED BY PHYTOPATHOGENIC MICROORGANISMS | |
| KR101288414B1 (en) | Novel Rhodotorula graminis Y435 and formulation for controlling plant postharvest disease containing same | |
| Pandey et al. | Potential Bacillus strains having plant growth promoting and volatile activity against pathogens of Pea (Pisum sativum L.) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20201106 |