CN108395349A - A kind of composite biological fertilizer and preparation method thereof - Google Patents
A kind of composite biological fertilizer and preparation method thereof Download PDFInfo
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- CN108395349A CN108395349A CN201710070257.8A CN201710070257A CN108395349A CN 108395349 A CN108395349 A CN 108395349A CN 201710070257 A CN201710070257 A CN 201710070257A CN 108395349 A CN108395349 A CN 108395349A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
Abstract
The invention belongs to produce technical field of fertilizers, in particular to a kind of composite biological fertilizer and preparation method thereof.The composite biological fertilizer is prepared from the following materials：200 300 parts by weight of nitrogen, phosphorus and potassium fertilizer, 0.1 0.5 parts by weight of pseudomonas fluorescens bacterium solution, 0.1 0.5 parts by weight of Paecilomyces lilacinus bacterium solution, 0.1 0.5 parts by weight of Bacillus licheniformis liquid, 25 parts by weight of carrier, 37 parts by weight of strain protective agent.Each ingredient synergistic effect in the present invention; both it can guarantee increasing crop yield; reduce fertilizer application amount again; cost is reduced, soil physical property can be improved, is conducive to increase soil fertility; in its preparation process; strain protective agent is added, biological inoculum still is able to survive in the case of higher nitrogen, phosphorus, potassium content to ensure that, and keeps greater activity.
Description
Technical field
The invention belongs to produce technical field of fertilizers, in particular to a kind of composite biological fertilizer and preparation method thereof.
Background technology
China human mortality is more, arable land is few, and as population increases, industry and the development in city arable land are successively decreased year by year.According to statistics,
China human mortality increases by 16,000,000 every year on average, and ploughs and reduce 4,000,000 mu every year.Currently, China's per capita cultivated land's level is significantly low
In world per capita cultivated land level, also below Asia per capita cultivated land level.For this purpose, China increases the unique feasible way of agricultural output
Diameter is to increase the yield per unit area, and could only in this way meet the needs of industrial development and population increase are to agricultural product, chemical fertilizer
What is just followed a well mapped-out plan is widely applied.
Agricultural fertilizer includes chemical fertilizer and bio-feritlizer, is the irreplaceable important means of production of China's agricultural production,
It is unreasonable for a long time to use chemical fertilizer, cause soil hardening, soil fertility decline, ecological disruption, environmental pollution and agricultural and sideline
The problems such as deterioration in quality.Soil is mainly made of minerals, organic matter and microorganism three parts, edaphon
Activity, it is extremely important to plant root nutrition because the beneficial microbe in soil directly participates in the formation of soil fertility, packet
Include the conversion of matter and energy in soil, the formation of humus and decomposition, the release of nutrient, the fixation of nitrogen etc..But it is pure from
Quantity of useful microbe is inadequate under right state, and active force is also limited.Therefore, it is added into soil using artificial mode beneficial to micro- life
Object, it will be able to which the quantity for increasing Soil Microorganism increases the overall activity of microorganism, to significantly improve the fertility of soil.
Crop yield, reductionization can be made using microbial manure and chemical fertilizer compounding application according to China's crop species and edaphic condition
Fertile usage amount, reduces cost, moreover it is possible to improve soil and crop quality, reduce pollution.
Due to containing a large amount of biological bacteria, wherein beneficial bacterium proliferation in the soil and metabolism can inhibit composite bio-fertilizer
The growth of harmful bacteria, to mitigate the generation of corps diseases, for safeguarding that soil health also has certain effect.Pass through biological bacteria
Activity, can not only change the physicochemical character of soil, improve the content and transformation efficiency of the soil organism, and with solution
Potassium, the function of releasing phosphorus, fixed nitrogen.Bio-bacterial manure itself is nontoxic, noresidue, and can decompose the residual of chemical fertilizer in soil, pesticide, dissolves
Heavy-metal residual after sewage irrigation, purification and rehabilitating soil, improve crop yield and quality of agricultural product.
Manufacture high nutrient composite microbe fertilizer, it is necessary to be carried out after mixing microbial bacterial agent with nitrogen, phosphorus and potassium fertilizer, still, at present
In the composite biological fertilizer used, after being prepared as composite biological fertilizer, survival ability in preserving process declines strain, when survival
Between shorten, viable bacteria is dead quickly, does not have the effect of bacterial manure, produce and use during show as bacterial activity decline, production
Impaired performance, reproduction speed is slack-off, and resistance is poor.
Invention content
The technical problem to be solved in the present invention is to provide a kind of composite biological fertilizers and preparation method thereof.
Present disclosure includes：
A kind of composite biological fertilizer, is prepared from the following materials：
Nitrogen, phosphorus and potassium fertilizer 200-300 parts by weight
Pseudomonas fluorescens bacterium solution 0.1-0.5 parts by weight
Paecilomyces lilacinus bacterium solution 0.1-0.5 parts by weight
Bacillus licheniformis liquid 0.1-0.5 parts by weight
Carrier 2-5 parts by weight
Strain protective agent 3-7 parts by weight.
In the present invention, as a kind of perferred technical scheme, it is prepared from the following materials：
Nitrogen, phosphorus and potassium fertilizer 230-270 parts by weight
Pseudomonas fluorescens bacterium solution 0.2-0.3 parts by weight
Paecilomyces lilacinus bacterium solution 0.1-0.2 parts by weight
Bacillus licheniformis liquid 0.2-0.3 parts by weight
Carrier 4-5 parts by weight
Strain protective agent 4-5 parts by weight.
In the present invention, as a kind of perferred technical scheme, the carrier is talcum powder or diatomite.
In the present invention, as a kind of perferred technical scheme, the strain protective agent is dimethyl sulfoxide (DMSO), carboxymethyl cellulose
One or more of plain sodium, sorbierite, mannitol, trehalose, sodium glutamate or phosphate.
In inventive formulation：
Pseudomonas fluorescens have good promoting root growth growth result, and can prevent and inhibit tomato, eggplant, capsicum, cigarette
The generation of the soil-borne diseases such as bacterial wilt, the bacterial wilt of ginger of the solanaceous crops such as grass, and with the sprouting of promotion seed, raising germinating energy and go out
The advantages that seedling rate.
Paecilomyces lilacinus belongs to endoparasitism fungi, is the Important Natural Enemy of some plant nematodes, can parasitize
Ovum can also infect larva and female adult, can the nemas such as substantially reduced various crop root-knot nematode, cyst nematode, Ditylenchus dipsaci
Harm；Also there is good fungistatic effect to various agricultural pathogenic bacteria.
Bacillus licheniformis can promote body to generate antibacterial substance, kill pathogenic bacteria.Resistant activity material can be generated,
And there is unique biology take oxygen mechanism, the growth and breeding of pathogenic bacteria can be inhibited.
The survival rate of strain can be improved in strain protective agent, extends the time-to-live of strain.
A kind of preparation method of composite biological fertilizer, includes the following steps：
(1) preparation of pseudomonas fluorescens bacterium solution
Pseudomonas fluorescence is inoculated into Jin Shi B culture mediums, in 30~35 DEG C, 150r/min shaken cultivations 18~20
Hour, then by Pseudomonas fluorescence streak inoculation to Jin Shi B culture medium flat plates, 18 are cultivated in 30~35 DEG C of incubators
~20 hours, picking single bacterium colony was inoculated into again in Jin Shi B culture mediums, in 30~35 DEG C, 150r/min shaken cultivations 18~20
Hour, Pseudomonas fluorescence culture solution is obtained, Pseudomonas fluorescence culture solution is inoculated into Jin Shi B culture mediums, in 30~
35 DEG C, 150r/min shaken cultivations 18~20 hours, obtain Pseudomonas fluorescence seed liquor, then by Pseudomonas fluorescence kind
Sub- liquid is inoculated into the fermentation tank containing Jin Shi B culture mediums, is continuously cultivated 24~30 hours, is obtained Pseudomonas fluorescence liquid；
(2) preparation of Paecilomyces lilacinus bacterium solution
Paecilomyces lilacinus is accessed into PDA liquid medium, under the conditions of 25~28 DEG C, 60~120r/min on shaking table
Shaken cultivation 48~72 hours, will acquisition culture solution dilute after be applied on Solid media for plates, in 25~28 DEG C, 60~
It is cultivated 3~5 days under the conditions of 120r/min, after growing sturdy mycelia, selects sturdy mycelia and be transferred to solid slope culture medium, trained
It supports and obtains primary inclined plane strain, the primary inclined plane strain is accessed into PDA liquid medium, in 25~28 DEG C, 60~120r/
It is cultivated 48~72 hours under the conditions of min, obtains second-level shake flask seed liquor, the second-level shake flask seed liquor is accessed into PDA Liquid Cultures
Base ferments 3~5 days to get Paecilomyces lilacinus bacterium solution under the conditions of PH7.0~7.2,25~28 DEG C；
(3) preparation of Bacillus licheniformis liquid
By bacillus licheniformis in 20~35 DEG C, under confined conditions, inoculation, activation culture 10~36 in activation medium
Hour, expansion numerous 24~72 that then cultured strain ferments in the fermentation medium at PH7.0~7.2,20~35 DEG C of temperature
Hour, it constantly shakes or is stirred to get Bacillus licheniformis liquid during the activation culture and fermentation expansion are numerous；
(4) preparation of composite bacteria agent：By the pseudomonas fluorescens bacterium solution of above-mentioned gained, Paecilomyces lilacinus bacterium solution
With Bacillus licheniformis liquid respectively through carrier adsorption, drying, crushing, pseudomonas fluorescens bacterium powder, Paecilomyces lilacinus are obtained
Bacterium powder and Bacillus licheniformis powder, by three kinds of bacterium powders, mixing obtains complex micro organism fungicide；
(5) preparation of composite biological fertilizer：The complex micro organism fungicide is mixed with nitrogen, phosphorus and potassium fertilizer and strain protective agent
Uniformly to get.
In the present invention, as a kind of perferred technical scheme, in step (3), the activation medium is peptone, beef
Leach object, sodium chloride, agar and sterile water；
In the present invention, as a kind of perferred technical scheme, the peptone, beef leaching object, sodium chloride, agar and nothing
The mass percent of bacterium water is 8: 3: 5: 13: 1000.
In the present invention, as a kind of perferred technical scheme, in step (3), the fermentation medium is peptone, beef
Cream, magnesium chloride, corn flour, sucrose, starch, ferrous sulfate, potassium dihydrogen phosphate and sterile water.
In the present invention, as a kind of perferred technical scheme, the peptone, beef extract, magnesium chloride, corn flour, sucrose,
Starch, ferrous sulfate, potassium dihydrogen phosphate and sterile water mass percent be 180: 8: 4: 400: 300: 100: 8: 5: 2000.
The invention has the advantages that
Composite biological fertilizer provided by the invention, each ingredient synergistic effect, not only can guarantee increasing crop yield, but also reduce in side
Fertilizer application amount, reduces cost, composite biological fertilizer can generate the glucide for accounting for the soil organism 0.1%, carbohydrate object
Matter is combined together with plant mucilage, mineral idiosome and organic colloid, can improve soil aggregate structure, enhances the physics of soil
Performance and the loss for reducing soil particle, under certain conditions, moreover it is possible to participate in humus and be formed, so the composite biological fertilizer
Soil physical property can be improved, be conducive to increase soil fertility；In its preparation process, strain protective agent is added, to ensure
Biological inoculum still is able to survive higher nitrogen, phosphorus, the potassium content in the case of, and keeps greater activity.
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment 1
A kind of composite biological fertilizer, is prepared from the following materials：
200 parts by weight of nitrogen, phosphorus and potassium fertilizer
0.5 parts by weight of pseudomonas fluorescens bacterium solution
0.1 parts by weight of Paecilomyces lilacinus bacterium solution
0.3 parts by weight of Bacillus licheniformis liquid
2 parts by weight of carrier
3 parts by weight of strain protective agent.
Wherein, carrier is diatomite, and strain protective agent is mannitol.
Its preparation process is as follows：
(1) preparation of pseudomonas fluorescens bacterium solution
Pseudomonas fluorescence is inoculated into Jin Shi B culture mediums, in 35 DEG C, 150r/min shaken cultivations 18 hours, so
Afterwards by Pseudomonas fluorescence streak inoculation to Jin Shi B culture medium flat plates, cultivated 18 hours in 35 DEG C of incubators, picking list
Bacterium colony is inoculated into again in Jin Shi B culture mediums, in 35 DEG C, 150r/min shaken cultivations 18 hours, obtains Pseudomonas fluorescence training
Pseudomonas fluorescence culture solution is inoculated into Jin Shi B culture mediums by nutrient solution, in 35 DEG C, 150r/min shaken cultivations 18 hours,
Pseudomonas fluorescence seed liquor is obtained, Pseudomonas fluorescence seed liquor is then inoculated into the fermentation containing Jin Shi B culture mediums
In tank, continuous culture 30 hours obtains Pseudomonas fluorescence liquid；
(2) preparation of Paecilomyces lilacinus bacterium solution
Paecilomyces lilacinus is accessed into PDA liquid medium, under the conditions of 25 DEG C, 120r/min on shaking table shaken cultivation
It 48 hours, is applied on Solid media for plates after the culture solution of acquisition is diluted, 3 is cultivated under the conditions of 25 DEG C, 120r/min
It selects sturdy mycelia and is transferred to solid slope culture medium after growing sturdy mycelia, and culture obtains primary inclined plane strain, will
The primary inclined plane strain accesses PDA liquid medium, is cultivated 48 hours under the conditions of 25 DEG C, 120r/min, obtains second-level shake flask
Second-level shake flask seed liquor is accessed PDA liquid medium by seed liquor, and 3 days are fermented under the conditions of PH7.0,25 DEG C to get pale purple quasi-
Penicillium notatum bacterium solution；
(3) preparation of Bacillus licheniformis liquid
By bacillus licheniformis in 20 DEG C, under confined conditions, inoculation, activation culture 36 hours in activation medium are living
It is peptone, beef leaching object, sodium chloride, agar and the sterile water that mass percent is 8: 3: 5: 13: 1000 to change culture medium, so
Cultured strain is fermented in the fermentation medium at PH7.0,20 DEG C afterwards and is expanded numerous 72 hours, fermentation medium is quality hundred
Divide peptone, beef extract, magnesium chloride, corn flour, sucrose, starch, the sulphur than being 180: 8: 4: 400: 300: 100: 8: 5: 2000
Sour ferrous, potassium dihydrogen phosphate and sterile water constantly shake during the activation culture and fermentation expansion are numerous or stir to get ground
Clothing bacillus bacterium solution；
(4) preparation of composite bacteria agent：By the pseudomonas fluorescens bacterium solution of above-mentioned gained, Paecilomyces lilacinus bacterium solution
With Bacillus licheniformis liquid respectively through carrier adsorption, drying, crushing, pseudomonas fluorescens bacterium powder, Paecilomyces lilacinus are obtained
Bacterium powder and Bacillus licheniformis powder, by three kinds of bacterium powders, mixing obtains complex micro organism fungicide；
(5) preparation of composite biological fertilizer：The complex micro organism fungicide is mixed with nitrogen, phosphorus and potassium fertilizer and strain protective agent
Uniformly to get.
Embodiment 2
A kind of composite biological fertilizer, is prepared from the following materials：
250 parts by weight of nitrogen, phosphorus and potassium fertilizer
0.3 parts by weight of pseudomonas fluorescens bacterium solution
0.2 parts by weight of Paecilomyces lilacinus bacterium solution
0.1 parts by weight of Bacillus licheniformis liquid
4 parts by weight of carrier
7 parts by weight of strain protective agent.
Wherein, carrier is talcum powder, and strain protective agent is sodium carboxymethylcellulose.
Its preparation process is as follows：
(1) preparation of pseudomonas fluorescens bacterium solution
Pseudomonas fluorescence is inoculated into Jin Shi B culture mediums, in 30 DEG C, 150r/min shaken cultivations 20 hours, so
Afterwards by Pseudomonas fluorescence streak inoculation to Jin Shi B culture medium flat plates, cultivated 20 hours in 30 DEG C of incubators, picking list
Bacterium colony is inoculated into again in Jin Shi B culture mediums, in 30 DEG C, 150r/min shaken cultivations 20 hours, obtains Pseudomonas fluorescence training
Pseudomonas fluorescence culture solution is inoculated into Jin Shi B culture mediums by nutrient solution, in 30 DEG C, 150r/min shaken cultivations 20 hours,
Pseudomonas fluorescence seed liquor is obtained, Pseudomonas fluorescence seed liquor is then inoculated into the fermentation containing Jin Shi B culture mediums
In tank, continuous culture 24 hours obtains Pseudomonas fluorescence liquid；
(2) preparation of Paecilomyces lilacinus bacterium solution
Paecilomyces lilacinus is accessed into PDA liquid medium, under the conditions of 26 DEG C, 80r/min on shaking table shaken cultivation
It 60 hours, is applied on Solid media for plates after the culture solution of acquisition is diluted, 5 is cultivated under the conditions of 26 DEG C, 80r/min
It selects sturdy mycelia and is transferred to solid slope culture medium after growing sturdy mycelia, and culture obtains primary inclined plane strain, will
The primary inclined plane strain accesses PDA liquid medium, is cultivated 60 hours under the conditions of 26 DEG C, 80r/min, obtains second-level shake flask kind
Second-level shake flask seed liquor is accessed PDA liquid medium, 5 days is fermented under the conditions of PH7.1,26 DEG C to get pale purple quasi- blueness by sub- liquid
Mould bacterium solution；
(3) preparation of Bacillus licheniformis liquid
By bacillus licheniformis in 28 DEG C, under confined conditions, inoculation, activation culture 24 hours in activation medium are living
It is peptone, beef leaching object, sodium chloride, agar and the sterile water that mass percent is 8: 3: 5: 13: 1000 to change culture medium, so
Cultured strain is fermented in the fermentation medium at PH7.0,28 DEG C afterwards and is expanded numerous 36 hours, fermentation medium is quality hundred
Divide peptone, beef extract, magnesium chloride, corn flour, sucrose, starch, the sulphur than being 180: 8: 4: 400: 300: 100: 8: 5: 2000
Sour ferrous, potassium dihydrogen phosphate and sterile water constantly shake during the activation culture and fermentation expansion are numerous or stir to get ground
Clothing bacillus bacterium solution；
(4) preparation of composite bacteria agent：By the pseudomonas fluorescens bacterium solution of above-mentioned gained, Paecilomyces lilacinus bacterium solution
With Bacillus licheniformis liquid respectively through carrier adsorption, drying, crushing, pseudomonas fluorescens bacterium powder, Paecilomyces lilacinus are obtained
Bacterium powder and Bacillus licheniformis powder, by three kinds of bacterium powders, mixing obtains complex micro organism fungicide；
(5) preparation of composite biological fertilizer：The complex micro organism fungicide is mixed with nitrogen, phosphorus and potassium fertilizer and strain protective agent
Uniformly to get.
Embodiment 3
A kind of composite biological fertilizer, is prepared from the following materials：
300 parts by weight of nitrogen, phosphorus and potassium fertilizer
0.1 parts by weight of pseudomonas fluorescens bacterium solution
0.1 parts by weight of Paecilomyces lilacinus bacterium solution
0.5 parts by weight of Bacillus licheniformis liquid
3 parts by weight of carrier
4 parts by weight of strain protective agent.
Wherein, carrier is talcum powder, and strain protective agent is dimethyl sulfone.
Its preparation process is as follows：
(1) preparation of pseudomonas fluorescens bacterium solution
Pseudomonas fluorescence is inoculated into Jin Shi B culture mediums, in 33 DEG C, 150r/min shaken cultivations 19 hours, so
Afterwards by Pseudomonas fluorescence streak inoculation to Jin Shi B culture medium flat plates, cultivated 19 hours in 33 DEG C of incubators, picking list
Bacterium colony is inoculated into again in Jin Shi B culture mediums, in 33 DEG C, 150r/min shaken cultivations 19 hours, obtains Pseudomonas fluorescence training
Pseudomonas fluorescence culture solution is inoculated into Jin Shi B culture mediums by nutrient solution, in 33 DEG C, 150r/min shaken cultivations 19 hours,
Pseudomonas fluorescence seed liquor is obtained, Pseudomonas fluorescence seed liquor is then inoculated into the fermentation containing Jin Shi B culture mediums
In tank, continuous culture 26 hours obtains Pseudomonas fluorescence liquid；
(2) preparation of Paecilomyces lilacinus bacterium solution
Paecilomyces lilacinus is accessed into PDA liquid medium, under the conditions of 28 DEG C, 60r/min on shaking table shaken cultivation
It 72 hours, is applied on Solid media for plates after the culture solution of acquisition is diluted, 4 is cultivated under the conditions of 28 DEG C, 60r/min
It selects sturdy mycelia and is transferred to solid slope culture medium after growing sturdy mycelia, and culture obtains primary inclined plane strain, will
The primary inclined plane strain accesses PDA liquid medium, is cultivated 72 hours under the conditions of 28 DEG C, 60r/min, obtains second-level shake flask kind
Second-level shake flask seed liquor is accessed PDA liquid medium, 4 days is fermented under the conditions of PH7.2,28 DEG C to get pale purple quasi- blueness by sub- liquid
Mould bacterium solution；
(3) preparation of Bacillus licheniformis liquid
By bacillus licheniformis in 35 DEG C, under confined conditions, inoculation, activation culture 10 hours in activation medium are living
It is peptone, beef leaching object, sodium chloride, agar and the sterile water that mass percent is 8: 3: 5: 13: 1000 to change culture medium, so
Cultured strain is fermented in the fermentation medium at PH7.0,28 DEG C afterwards and is expanded numerous 48 hours, fermentation medium is quality hundred
Divide peptone, beef extract, magnesium chloride, corn flour, sucrose, starch, the sulphur than being 180: 8: 4: 400: 300: 100: 8: 5: 2000
Sour ferrous, potassium dihydrogen phosphate and sterile water constantly shake during the activation culture and fermentation expansion are numerous or stir to get ground
Clothing bacillus bacterium solution；
(4) preparation of composite bacteria agent：By the pseudomonas fluorescens bacterium solution of above-mentioned gained, Paecilomyces lilacinus bacterium solution
With Bacillus licheniformis liquid respectively through carrier adsorption, drying, crushing, pseudomonas fluorescens bacterium powder, Paecilomyces lilacinus are obtained
Bacterium powder and Bacillus licheniformis powder, by three kinds of bacterium powders, mixing obtains complex micro organism fungicide；
(5) preparation of composite biological fertilizer：The complex micro organism fungicide is mixed with nitrogen, phosphorus and potassium fertilizer and strain protective agent
Uniformly to get.
To carrying out living bacteria count detection, detection after composite biological fertilizer storage a period of time made from embodiment 1-3
As a result it see the table below：
As can be seen from the table, composite biological fertilizer provided by the invention, after storing a period of time, living bacteria count is not
But do not reduce, and than it is initial when living bacteria count increase it is very much, illustrate composite biological fertilizer provided by the invention
Biological inoculum still is able to survive in the case of higher nitrogen, phosphorus, potassium content, and can keep greater activity.
Those of ordinary skills in the art should understand that：The discussion of any of the above embodiment is exemplary only, not
It is intended to imply that the scope of the present disclosure (including claim) is limited to these examples；Under the thinking of the present invention, above example
Or can also be combined between the technical characteristic in different embodiments, step can be realized with random order, and be existed such as
Many other variations of the different aspect of the upper present invention, for simplicity, they are not provided in details.Therefore, it is all
Within the spirit and principles in the present invention, any omission, modification, equivalent replacement, improvement for being made etc. should be included in the present invention's
Within protection domain.
Claims (9)
1. a kind of composite biological fertilizer, characterized in that be prepared from the following materials：
Nitrogen, phosphorus and potassium fertilizer 200-300 parts by weight
Pseudomonas fluorescens bacterium solution 0.1-0.5 parts by weight
Paecilomyces lilacinus bacterium solution 0.1-0.5 parts by weight
Bacillus licheniformis liquid 0.1-0.5 parts by weight
Carrier 2-5 parts by weight
Strain protective agent 3-7 parts by weight.
2. composite biological fertilizer as described in claim 1, characterized in that be prepared from the following materials：
Nitrogen, phosphorus and potassium fertilizer 230-270 parts by weight
Pseudomonas fluorescens bacterium solution 0.2-0.3 parts by weight
Paecilomyces lilacinus bacterium solution 0.1-0.2 parts by weight
Bacillus licheniformis liquid 0.2-0.3 parts by weight
Carrier 4-5 parts by weight
Strain protective agent 4-5 parts by weight.
3. composite biological fertilizer as described in claim 1, characterized in that the carrier is talcum powder or diatomite.
4. composite biological fertilizer as described in claim 1, characterized in that the strain protective agent is dimethyl sulfoxide (DMSO), carboxylic first
One or more of base sodium cellulosate, sorbierite, mannitol, trehalose, sodium glutamate or phosphate.
5. a kind of preparation method of composite biological fertilizer, characterized in that include the following steps：
(1) preparation of pseudomonas fluorescens bacterium solution
Pseudomonas fluorescence is inoculated into Jin Shi B culture mediums, in 30~35 DEG C, 150r/min shaken cultivations 18~20 it is small
When, then will in Pseudomonas fluorescence streak inoculation to Jin Shi B culture medium flat plates, in 30~35 DEG C of incubators cultivate 18~
20 hours, picking single bacterium colony was inoculated into again in Jin Shi B culture mediums, in 30~35 DEG C, 150r/min shaken cultivations 18~20 it is small
When, Pseudomonas fluorescence culture solution is obtained, Pseudomonas fluorescence culture solution is inoculated into Jin Shi B culture mediums, in 30~35
DEG C, 150r/min shaken cultivations 18~20 hours, Pseudomonas fluorescence seed liquor is obtained, then by Pseudomonas fluorescence seed
Liquid is inoculated into the fermentation tank containing Jin Shi B culture mediums, is continuously cultivated 24~30 hours, is obtained Pseudomonas fluorescence liquid；
(2) preparation of Paecilomyces lilacinus bacterium solution
Paecilomyces lilacinus is accessed into PDA liquid medium, is vibrated on shaking table under the conditions of 25~28 DEG C, 60~120r/min
Culture 48~72 hours, will acquisition culture solution dilute after be applied on Solid media for plates, in 25~28 DEG C, 60~
It is cultivated 3~5 days under the conditions of 120r/min, after growing sturdy mycelia, selects sturdy mycelia and be transferred to solid slope culture medium, trained
It supports and obtains primary inclined plane strain, the primary inclined plane strain is accessed into PDA liquid medium, in 25~28 DEG C, 60~120r/
It is cultivated 48~72 hours under the conditions of min, obtains second-level shake flask seed liquor, the second-level shake flask seed liquor is accessed into PDA Liquid Cultures
Base ferments 3~5 days to get Paecilomyces lilacinus bacterium solution under the conditions of PH7.0~7.2,25~28 DEG C；
(3) preparation of Bacillus licheniformis liquid
By bacillus licheniformis in 20~35 DEG C, under confined conditions, in activation medium inoculation, activation culture 10~36 it is small
When, numerous 24~72 hours of expansion that then cultured strain ferments in the fermentation medium at PH7.0~7.2,20~35 DEG C,
It constantly shakes or is stirred to get Bacillus licheniformis liquid during the activation culture and fermentation expansion are numerous；
(4) preparation of composite bacteria agent：By the pseudomonas fluorescens bacterium solution, Paecilomyces lilacinus bacterium solution and ground of above-mentioned gained
Clothing bacillus bacterium solution through carrier adsorption, drying, crushing, obtains pseudomonas fluorescens bacterium powder, Paecilomyces lilacinus bacterium powder respectively
And Bacillus licheniformis powder, by three kinds of bacterium powders, mixing obtains complex micro organism fungicide；
(5) preparation of composite biological fertilizer：The complex micro organism fungicide is uniformly mixed with nitrogen, phosphorus and potassium fertilizer and strain protective agent,
To obtain the final product.
6. the preparation method of composite biological fertilizer as claimed in claim 5, characterized in that in step (3), the activation culture
Base is peptone, beef leaching object, sodium chloride, agar and sterile water.
7. the preparation method of composite biological fertilizer as claimed in claim 6, characterized in that the peptone, beef leaching object,
The mass percent of sodium chloride, agar and sterile water is 8: 3: 5: 13: 1000.
8. the preparation method of composite biological fertilizer as claimed in claim 5, characterized in that in step (3), the fermented and cultured
Base is peptone, beef extract, magnesium chloride, corn flour, sucrose, starch, ferrous sulfate, potassium dihydrogen phosphate and sterile water.
9. the preparation method of composite biological fertilizer as claimed in claim 8, characterized in that the peptone, beef extract, chlorination
Magnesium, corn flour, sucrose, starch, ferrous sulfate, potassium dihydrogen phosphate and sterile water mass percent be 180: 8: 4: 400: 300:
100∶8∶5∶2000。
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110467501A (en) * | 2019-08-29 | 2019-11-19 | 大连大学 | A method of selenium-rich organic Herba portulacae is planted by base manure of microbial liquid microbial inoculum |
| CN112980738A (en) * | 2021-04-09 | 2021-06-18 | 中国科学院沈阳应用生态研究所 | Compound microbial fertilizer for preventing and treating root-knot nematode and preparation method thereof |
| CN113444645A (en) * | 2021-04-26 | 2021-09-28 | 上海又然生态科技有限公司 | Multifunctional composite fermentation inoculant and preparation method and application thereof |
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| CN113444645A (en) * | 2021-04-26 | 2021-09-28 | 上海又然生态科技有限公司 | Multifunctional composite fermentation inoculant and preparation method and application thereof |
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