CN102173954A - Preparation method of microbial-ecological foliar fertilizer promoting plant growth and controlling pest - Google Patents
Preparation method of microbial-ecological foliar fertilizer promoting plant growth and controlling pest Download PDFInfo
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- CN102173954A CN102173954A CN201110069142XA CN201110069142A CN102173954A CN 102173954 A CN102173954 A CN 102173954A CN 201110069142X A CN201110069142X A CN 201110069142XA CN 201110069142 A CN201110069142 A CN 201110069142A CN 102173954 A CN102173954 A CN 102173954A
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Abstract
The invention discloses a preparation method of a microbial-ecological foliar fertilizer promoting plant growth and controlling pest. The method comprises the following steps: separately cultivating bacillus, lactic acid bacteria, rhodopseudomonas palustris with the cell concentration of over 10 billion/ml and a bacteria liquid of yeast with the cell concentration of over 2 billion/ml; and mixing the mixed solution of the four kinds of probiotics with abamectin of a certain proportion to obtain the liquid microbial-ecological foliar fertilizer with the total cell concentration of over 8 billion/ml. The method disclosed by the invention has the advantages that: all strains adopted are edible probiotics of human beings, safe and nontoxic; abamectin is a macrolides insecticidal antibiotic agent, and has the characteristics of high efficiency, low toxicity, no pollution, lasting effect and the like; the prepared microbial-ecological foliar fertilizer integrates the advantages of probiotics in promoting plant growth and abamectin in efficient pest control; the microbial-ecological foliar fertilizer is diluted with water for 100 times and sprayed onto the leaf surfaces of the plant every other 7-10 days; and the microbial-ecological foliar fertilizer not only obviously increases the plant yield by over 30%, but also can control plant pest, thereby having important application value.
Description
Technical field
The invention belongs to biological technical field, particularly provide a kind of probiotic bacterium and Avrmectin of utilizing to prepare the method that promotes plant-growth and the little ecological foliage fertilizer of pest control.
Background technology
Foliage fertilizer refers to impose on the fertilizer that plant leaf also can be absorbed utilization, because foliage fertilizer can directly enter in the plant materials from leaf portion, the metabolism of involved in plant and organic building-up process, therefore in the time of a little less than growth and development of plants later stage root system receptivity, can in time remedy required nutritive substance.Foliage fertilizer as emerging fertilizer representative can improve the yield and quality of crop, and its research and application more and more have been subjected to people's generally attention.
Foliage fertilizer originates from France the earliest, from botanist Grisb in 1884 FeSO
4Solution has spread upon on the jaundice grape leave in order to since correcting the yellowtop that causes because of iron deficiency, and foliage fertilizer is used in production practice and research has had significant progress.One of agrochemistry originator's Byccehro thinks, leaf can absorb nutritive substance and magnesium and potassium etc. in the plant as root when absorbing water droplet and go.China peasant of Zhejiang Province begins the rice application foliage fertilizer before more than 200 years, has opened up the precedent of China's application foliage fertilizer.Middle 1980s, China began to research and produce the novel foliage fertilizer that different sorts contains plant-growth regulator or farm chemical ingredients, made the function variation more of foliage fertilizer.After nineteen nineties, some novel foliage fertilizers application effect in the plantation of cash crop such as watermelon, citrus and grape is very remarkable.Along with foliage fertilizer research and going deep into of using, the type of foliage fertilizer is enriched constantly, and kind constantly increases, and from initial single type foliage fertilizer, develops into present multiclass composite foliage fertilizer.At present, the nutritious type foliage fertilizer of the main type of foliage fertilizer, adjustment type foliage fertilizer, microbiocidal foliage fertilizer, single type microelement foliage fertilizer and composite foliage fertilizer etc., wherein microorganism foliage fertilizer is a dark horse, and becomes the outstanding person in the foliage fertilizer.Microorganism foliage fertilizer contains microbial cells and metabolite thereof, can stimulate plant growth, promotes crop metabolism, alleviates and prevent and treat the generation of disease.But do not report aspect little ecological foliage fertilizer adopting probiotic bacterium and biological pesticide to make up to produce.
Animal microecological formulation refers to use little ecological principle will be from isolating probiotic bacterium in the animal body, the active bacteria formulation of cultivating through special process.Probiotic bacterium (probiotics) speech is the earliest in order to describe " by a kind of material that can stimulate another kind of microorganism growth of microorganism secretion ".Probiotic bacterium was defined as " being of value to animal host's viable bacteria feed additive by improving the intestinal microflora balance " in 1989.The latest definition that European food in 2002 and feed association have provided probiotic bacterium for " probiotic bacterium is the microorganism that lives, by take in can to the host produce one or more special and through proving the functional health benefit ".Because probiotics can promote growth of animal, improve meat, egg and milk quality and be expected to replace microbiotic, become the focus and the forward position of domestic and international research and application,
But adopt probiotic bacterium rare as the research report in leaf leaven field.
Avrmectin (avermectins) is by streptomyces griseus in the soil microorganisms
Streptomyces avermitilisThe class that fermentation produces has desinsection, kills ten hexa-atomic Macrocyclic lactone compounds of mite, eelworm-killing activity.Avrmectin have tag, stomach toxicity and strong characteristics and the effect of seepage force, insect and mite class had tag and effect such as stomach toxicity.Avrmectin has very strong perviousness to plant leaf, can kill subepidermal insect, its mechanism of action mainly is to disturb the nervous physiology activity, stimulate to discharge arthropodan nerve conduction is had inhibiting γ-An Jidingsuan, paralysis symptom promptly appears after making various pests and medicament contacting, and dead after 2-4 days.Avrmectin all has very strong inhibitory or killing effect to the overwhelming majority's parasitic nematode and arthropods, becomes widely used biological pesticide.
At present, though adopt multiple probiotic bacterium to produce microorganism foliage fertilizer and adopt Avrmectin to prepare the biological pesticide aspect more extensive research is arranged all, with probiotic bacterium mixes with Avrmectin as promotion plant-growth and pest control novel little ecological foliage fertilizer aspect do not have to report.
Summary of the invention
In order to address the above problem, the purpose of this invention is to provide multiple probiotic bacterium of a kind of employing and Avrmectin produces and a kind ofly can either promote plant-growth, little ecological foliage fertilizer that again can the pest control safety non-toxic, by being sprayed on plant leaf surface, to reach the purpose that improves plant biomass and quality and pest control.
Technical scheme of the present invention: a kind of method of utilizing probiotic bacterium and Avrmectin to produce little ecological foliage fertilizer, it is characterized in that, comprise that specifically following work step is rapid:
At first, cultivate the acquisition cell concn respectively separately and reach 10,000,000,000/ml above bacillus natto, milk-acid bacteria and Rhodopseudomonas palustris, and cell concn reaches the above saccharomycetic liquid culture of 2,000,000,000/ml;
Secondly, to account for culture that volume percent is the bacillus natto of 15-25% respectively, account for culture that volume percent is the milk-acid bacteria of 15-25%, account for culture that volume percent is the Rhodopseudomonas palustris of 25-45%, account for volume percent is that the saccharomycetic culture of 15-25% carries out uniform mixing, the Avrmectin that adds percent weight in volume 0.2 ‰-2 ‰ again can obtain total cell concn and reach the above little ecological foliage fertilizer of liquid high value of 8,000,000,000/ml.
The invention has the advantages that: 1) used probiotic bacterium bacterial classification all is human edible probiotic bacterium, safety non-toxic.2) little ecological foliage fertilizer collection probiotic bacterium promotes the advantage of plant-growth and the effective pest control of Avrmectin to gather together.3) Avrmectin is the tunning of soil microorganisms, has the characteristics of efficient, low toxicity, nuisanceless, lasting medicine.4) after being sprayed on 100 times of little ecological foliage fertilizer dilutions on the plant leaf surface in 7-10 days, not only obviously prevent and treat the generation of insect pest of the plant about 60 days, and significantly improve plant biomass and reach 30%.Have important use is worth in fields such as Organic food, vegetables, flowers, fruit, tealeaves and leaf tobacco production.
Description of drawings
Fig. 1 obtains cell concn figure in the different probiotic culture respectively in the preparation process of specific embodiments of the invention 1.
Fig. 2 is the total cell concn figure that obtains little ecological foliage fertilizer in the preparation process of specific embodiments of the invention 1.
Fig. 3 is the figure that the little ecological foliage fertilizer of specific embodiments of the invention 1 production improves little growth of rape speed.
Fig. 4 is that the little ecological foliage fertilizer that specific embodiments of the invention 1 are produced is prevented and treated the figure that little rape insect pest takes place.
Embodiment
Below in conjunction with specific embodiment technical scheme of the present invention is described further.
Principle of the present invention is: probiotic culture comprises mycetocyte and metabolite thereof, possesses stimulating plant growth and metabolism, alleviates and prevents disease generation function.Avrmectin be a kind of with stomach poison function be advocate peace the Biocidal that has action of contace poison concurrently, kill mite, nematocides, it causes death the insect paralysis by hindering the transmission of pest movements nerve signal; Have efficient, low toxicity, nuisanceless, lasting medicine, be difficult for developing immunity to drugs, use advantage such as no cross resistance with other agricultural chemicals.The little ecological foliage fertilizer of producing after probiotic culture and Avrmectin mixed according to a certain percentage, the advantage of probiotic bacterium and meta-bolites raising plant-growth and the effective pest control of Avrmectin is combined, can increase substantially the speed of growth and the resistance against diseases of plant, reduce the generation of insect pest simultaneously.
Embodiment 1
Fermentation culture obtains cell concn and is respectively 18,200,000,000/ml bacillus natto at first separately, 15,700,000,000/ml lactobacillus, the liquid culture of 10,300,000,000/ml Rhodopseudomonas palustris and 2,800,000,000/ml S. cervisiae, as shown in Figure 1, with cell concn respectively 18,200,000,000/ml bacillus natto 100ml, 15,700,000,000/ml lactobacillus 100ml, 10,300,000,000/ml Rhodopseudomonas palustris 100ml and 2,800,000,000/ml S. cervisiae 100m mix, add 0.5 ‰ Avrmectin then, having obtained total cell concn is 117.5 hundred million/ml, Avrmectin content is 0.5 ‰ little ecological foliage fertilizer, as shown in Figure 2.Experimental group will produce by 4 kinds of probiotic bacteriums and contain the little ecological foliage fertilizer that 0.5 ‰ Avrmectins are formed, behind 100 times of the dilute with waters, the method that evenly was sprayed on the little rape blade face 1 time in 7 days according to every interval is used, control group then sprays the water of same dose, treat that little growth of rape was got respectively in 40 days and spray to use and do not spray each 50 the little rape that uses little ecological foliage fertilizer and weigh, the result shows that spraying average every the weight of the little rape that uses little ecological foliage fertilizer is 137.4 grams, and do not spray average every the weight of the little rape that uses little ecological blade face only is 100.7 grams, as shown in Figure 3, use the little rape increase output of little ecological foliage fertilizer to reach 36.4%.The main insect pest that influences little growth of rape is cabbage caterpillar and small cabbage moth, and every ground (20m is adopted in this research
2) 5 samplings of diagonal lines, 5 little rapes of every fixing investigation amount to borer population amount alive (the every ground 20m before the investigation on 25 little rapes of investigation
2The worm radix be no less than 100), control time is before foliage fertilizer sprays and sprayed back 1 day, 3 days and 7 days.The result shows, the number of pest of spraying the every ground of control group of same dose water rises to 163 from 104, and the number of pest of spraying the every ground of experimental group of foliage fertilizer then is reduced to 16 from 107, and as shown in Figure 4, the pest control rate is up to 85.0%.
Embodiment 2
Fermentation culture obtains cell concn and is respectively 17,700,000,000/ml bacillus natto, 15,700,000,000/ml lactobacillus, 10,200,000,000/ml Rhodopseudomonas palustris and 2,500,000,000/ml S. cervisiae totally 4 kinds of liquid cultures at first separately, mix according to the ratio that respectively accounts for total volume percent 15%, 15%, 45% and 25% respectively, add 0.2 ‰ Avrmectin then, having obtained total cell concn is 102.3 hundred million/ml, and Avrmectin content is 0.2 ‰ little ecological foliage fertilizer.
Embodiment 3
Fermentation culture obtains cell concn and is respectively 19,000,000,000/ml bacillus natto, 16,300,000,000/ml lactobacillus, 10,500,000,000/ml Rhodopseudomonas palustris and 2,700,000,000/ml S. cervisiae totally 4 kinds of liquid cultures at first separately, mix according to the ratio that respectively accounts for total volume percent 25%, 25%, 35% and 15% respectively, add 1.5 ‰ Avrmectin then, having obtained total cell concn is 129.1 hundred million/ml, and Avrmectin content is 1.5 ‰ little ecological foliage fertilizer.
Claims (1)
1. a preparation method who promotes plant-growth and the little ecological foliage fertilizer of pest control is characterized in that, comprises that specifically following work step is rapid:
At first, cultivate the acquisition cell concn respectively separately and reach 10,000,000,000/ml above bacillus natto, milk-acid bacteria and Rhodopseudomonas palustris, and cell concn reaches the above saccharomycetic liquid culture of 2,000,000,000/ml;
Secondly, to account for volume percent respectively and be 15-25% bacillus natto culture, to account for culture that volume percent is the milk-acid bacteria of 15-25%, account for culture that volume percent is the Rhodopseudomonas palustris of 25-45%, account for volume percent be that the saccharomycetic culture of 15-25% carries out uniform mixing, the Avrmectin that adds percent weight in volume 0.2 ‰-2 ‰ again can obtain total cell concn and reach the above little ecological foliage fertilizer of liquid high value of 8,000,000,000/ml.
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Cited By (4)
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| CN102775233A (en) * | 2012-08-06 | 2012-11-14 | 定襄县神宇科技示范基地有限公司 | Novel insecticidal and antibacterial biofertilizer and preparation method thereof |
| CN103058716A (en) * | 2013-01-14 | 2013-04-24 | 广西穗宁化工集团有限公司 | Organic matter liquid suitable for being used as leaf fertilizer raw material |
| CN103319223A (en) * | 2012-03-19 | 2013-09-25 | 韩嵩 | Foliar fertilizer special for capsicum and plantation method thereof |
| CN107974423A (en) * | 2017-12-25 | 2018-05-01 | 广东杰士农业科技有限公司 | A kind of biological soil activating agent and preparation method thereof |
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| CN101816254A (en) * | 2010-05-11 | 2010-09-01 | 沈阳化工学院 | Method for enhancing weak light resistance of cucumbers by spraying compound bacterial manure on foliage |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103319223A (en) * | 2012-03-19 | 2013-09-25 | 韩嵩 | Foliar fertilizer special for capsicum and plantation method thereof |
| CN103319223B (en) * | 2012-03-19 | 2017-08-29 | 庆阳敦博科技发展有限公司 | A kind of Foliar fertilizer special for capsicum and plantation method thereof |
| CN102775233A (en) * | 2012-08-06 | 2012-11-14 | 定襄县神宇科技示范基地有限公司 | Novel insecticidal and antibacterial biofertilizer and preparation method thereof |
| CN102775233B (en) * | 2012-08-06 | 2014-09-03 | 定襄县神宇科技示范基地有限公司 | Novel insecticidal and antibacterial biofertilizer and preparation method thereof |
| CN103058716A (en) * | 2013-01-14 | 2013-04-24 | 广西穗宁化工集团有限公司 | Organic matter liquid suitable for being used as leaf fertilizer raw material |
| WO2014108099A1 (en) * | 2013-01-14 | 2014-07-17 | 佛山市新新叶面肥料有限公司 | Organic liquid suitable for being used as raw material for foliar fertilizers |
| CN107974423A (en) * | 2017-12-25 | 2018-05-01 | 广东杰士农业科技有限公司 | A kind of biological soil activating agent and preparation method thereof |
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