CN110402929A - A kind of bactericidal composition and its application containing jinggangmeisu - Google Patents
A kind of bactericidal composition and its application containing jinggangmeisu Download PDFInfo
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- CN110402929A CN110402929A CN201910773214.5A CN201910773214A CN110402929A CN 110402929 A CN110402929 A CN 110402929A CN 201910773214 A CN201910773214 A CN 201910773214A CN 110402929 A CN110402929 A CN 110402929A
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- jinggangmeisu
- bacillus
- jila
- jila bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
Abstract
The present invention provides a kind of bactericidal compositions containing jinggangmeisu, belong to cultural control technical field, and the compound includes jinggangmeisu and Te Jila bacillus；The jinggangmeisu and the mass ratio of Te Jila bacillus are (1~15): (1~15)；The living bacteria count of the Te Jila bacillus is in 100,000,000,000 cfu/g or more.The present invention will be synergistic obvious after jinggangmeisu and Te Jila bacillus mixture, can increase substantially drug effect, reduce Pesticide use amount and drug cost.Meanwhile bactericidal composition provided by the invention can improve the lasting period of jinggangmeisu.
Description
Technical field
The invention belongs to cultural control technical field more particularly to a kind of bactericidal composition containing jinggangmeisu and its answer
With.
Background technique
Jinggangmeisu is also known as valida, is a kind of antibiotic that actinomyces generate, has stronger absorbability, easily by bacterium
Body cell is absorbed and is conducted rapidly in it, interferes and inhibit somatic cells growth and development.Belong to less toxic fungicide.Sterling size
Mouse acute oral LD502000 mgs/kg are all larger than, LD is subcutaneously injected50It is all larger than 1500 mgs/kg.5000 milligrams/public
Jin smears rat skin without toxic reaction.To fish low toxicity, carp TLM (96h) LD50> 40 mg/litres.
Te Jila bacillus is the biocontrol bacteria obtained from ocean.Te Jila bacillus can not only effectively inhibit
The growth of pathogen, and stimulation plant growth can be generated and induce the compound of plant defense response, soil root can be improved
The microenvironment of system enhances the growing way and disease resistance of plant, improves crop yield and income.
Jinggangmeisu and Te Jila bacillus have the advantages that small toxicity as biological pesticide, but its disadvantage is also very bright
Aobvious, the jinggangmeisu controlling plant diseases lasting period is short, and Te Jila bacillus controlling plant diseases drug effect is poor, and therefore, prevention and treatment is planted
The share of chemical pesticide is increasing in the medicament of object disease, but chemical pesticide toxicity is bigger than biological pesticide, influences food safety,
Therefore, it needs to develop the biological bactericide that a kind of effect is good, and toxicity is low, and the lasting period is grown.
Summary of the invention
In view of this, being used the purpose of the present invention is to provide a kind of bactericidal composition containing jinggangmeisu and its application
Bactericidal composition provided by the invention not only increases the preventive effect to plant disease, also extends the prevention and treatment time.
In order to achieve the above-mentioned object of the invention, the present invention provides following technical schemes:
The present invention provides a kind of bactericidal composition containing jinggangmeisu, the compound includes jinggangmeisu and Te Jila
Bacillus；
The jinggangmeisu and the mass ratio of Te Jila bacillus are (1~15): (1~15)；
The living bacteria count of the Te Jila bacillus is in 100,000,000,000 cfu/g or more.
Preferably, the jinggangmeisu and the mass ratio of Te Jila bacillus are (1.5~5): (1.5~8).
The present invention also provides application of the bactericidal composition described in above-mentioned technical proposal in controlling plant diseases.
Preferably, the plant disease includes powdery mildew and/or wilt disease.
Preferably, the plant includes cucumber and/or watermelon.
The present invention also provides the Bactericidal composite groups described in above-mentioned technical proposal to prepare the application in pesticide.
Preferably, the dosage form of the pesticide includes aqueous suspension agent and/or wettable powder.
Preferably, mass percentage of the jinggangmeisu in pesticide is 4~30%.
Preferably, content of the Te Jila bacillus in pesticide is 5~2,000,000,000 cfu/g.
The present invention provides a kind of bactericidal composition containing jinggangmeisu, the compound includes jinggangmeisu and Te Jila
Bacillus；The jinggangmeisu and the mass ratio of Te Jila bacillus are (1~15): (1~15)；The Te Jila gemma
The living bacteria count of bacillus is in 100,000,000,000 cfu/g or more.The present invention will cooperate with after jinggangmeisu and Te Jila bacillus mixture
Synergy is obvious, can increase substantially drug effect, reduces Pesticide use amount and drug cost.Meanwhile Bactericidal composite provided by the invention
Object can improve the lasting period of jinggangmeisu.
Specific embodiment
The present invention provides a kind of bactericidal composition containing jinggangmeisu, the compound includes jinggangmeisu and Te Jila
Bacillus；The jinggangmeisu and the mass ratio of Te Jila bacillus are (1~15): (1~15)；The Te Jila gemma
The living bacteria count of bacillus is in 100,000,000,000 cfu/g or more.
The present invention also provides application of the bactericidal composition in controlling plant diseases.In the present invention, described
Plant disease preferably includes powdery mildew and/wilt disease.
In the present invention, when the powdery mildew is preferably powdery mildew of cucumber, the application is preferably specifically included: described to kill
Bacteria composition is administered for the first time in cucumber fruiting, carries out secondary application after 6~8d；The dose of the primary application and secondary application
Independent effective component folding 100% is 8~10g/ mus.In the present invention, the bactericidal composition is preferably mixed to form with water
Water slurry；The amount of application of the water slurry is preferably 50~70kg/ mus, more preferably 60kg/ mus.In the present invention,
The insecticide-applying way of the water slurry preferably sprays, and the present invention is not particularly limited the spraying method, using routine
The method sprayed when preventing and treating powdery mildew of cucumber.
In the present invention, when the plant disease is preferably watermelon blight, the application is preferably specifically included: described
Bactericidal composition is once administered in watermelon fruiting, and secondary application is carried out after 6~8d；The primary application and secondary application
The independent effective component folding 100% of dose is 8~10g/ mus.In the present invention, the bactericidal composition is preferably mixed with water
Form water slurry；The amount of application of the water slurry is preferably 50~70kg/ mus, more preferably 60kg/ mus.In the present invention
In, the insecticide-applying way of the water slurry preferably sprays, and the present invention is not particularly limited the spraying method, using normal
Advise the method sprayed when preventing and treating watermelon blight.
The present invention also provides the Bactericidal composite groups described in above-mentioned technical proposal to prepare the application in pesticide.In the present invention
In, the dosage form of the pesticide preferably includes aqueous suspension agent and/or wettable powder, but is not limited only to above two dosage form.At this
In invention, mass percentage of the jinggangmeisu in pesticide is preferably 4~30%, and more preferably 10~20%.At this
In invention, content of the Te Jila bacillus in pesticide is preferably 5~2,000,000,000 cfu/g, and more preferably 10~1,500,000,000
cfu/g.The present invention is not particularly limited the supplementary product kind for preparing pesticide needs and content, using jinggangmeisu and Te Ji
Bacillus is drawn to be subjected to supplementary product kind and dosage on pesticide.The present invention is not special to the preparation method of the pesticide
It limits, using the conventional preparation method for preparing different dosage forms.
In the present invention, the jinggangmeisu and the mass ratio of Te Jila bacillus are (1~15): (1~15), preferably
For (1.5~5): (1.5~8).
The present invention is not particularly limited the source of the jinggangmeisu, using conventional commercial product.
The present invention is not particularly limited the source of the Te Jila bacillus, using conventional bacterial classification.
The present invention is not particularly limited the preparation method of the bactericidal composition containing jinggangmeisu, and the well ridge is mould
Element and Te Jila bacillus are mixed using conventional method.The present invention does not have the preparation method of the Te Jila bacillus
There is particular determination, makes the living bacteria count of Te Jila bacillus in 100,000,000,000 cfu/g or more using customary preparation methods.
Technical solution provided by the invention is described in detail below with reference to embodiment, but they cannot be understood
For limiting the scope of the present invention.
Embodiment 1
Indoor toxicity test of the composite jinggangmycin to powdery mildew of cucumber
Wherein, cucumber powdery mildew bacteria is acquired by field, is cultivated through indoor purifying.
Test method: after prerun determines the effective inhibition concentration range of each medicament, by Te Jila bacillus (Te Jila bud
The living bacteria count of spore bacillus is in 100,000,000,000 cfu/g or more), jinggangmeisu and its mixture preparation press active constituent content gradient system
Column 5 concentration of dilution, are compared with sterile water, are measured using mycelial growth rate method, and sterilizing training is added in various concentration medicament
It supports in ware, every ware 1mL, adds the PDA culture medium 9mL that sterilizing is melted, mix well and plate is made, then the bacterium colony to spread cultivation is reached
At the bacteria cake of 0.5cm, every ware is a piece of, and 3 repetitions of every processing are placed in 28 DEG C of insulating boxs and cultivate, it is straight to cultivate 4 days measurement bacterium colonies
Diameter calculates mycelial growth inhibition rate, finds out virulence regression equation, calculates EC50, mycelial growth inhibition rate formula is as follows:
Mycelial growth inhibition rate (%)=(blank control colony diameter-chemicals treatment colony diameter)/blank control bacterium colony
Diameter * 100
The synergistic effect of pharmacy mix is evaluated with the co-toxicity coefficient method (CTC) of Sun Yunpei, i.e. CTC≤80 are antagonism work
With 80 < CTC < 120 are summation action, and CTC≤120 are synergistic effect.
1 jinggangmeisu of table compounds the indoor toxicity test table to powdery mildew of cucumber with Te Jila bacillus
As can be seen from Table 1, jinggangmeisu, EC of the Te Jila bacillus to powdery mildew of cucumber50Respectively 18.82mg/L
And 38.35mg/L, when jinggangmeisu is 15:1~1:15 with Te Jila bacillus mass ratio, co-toxicity coefficient is greater than 120, table
Reveal synergistic effect, when jinggangmeisu is 1:1 with Te Jila bacillus mass ratio, co-toxicity coefficient is maximum, and synergistic effect is most
It is good.
Embodiment 2
Jinggangmeisu and Te Jila bacillus (living bacteria count of Te Jila bacillus is in 100,000,000,000 cfu/g) are pressed
Weight ratio 15:1 compounding, carries out the field trial of powdery mildew of cucumber prevention and treatment, and different agents are made into corresponding dense by the requirement of according to the form below
Degree is administered for the first time in cucumber period, is administered for second after 7 days, and application twice, 7 days after application, 14 days, measures for 21 days altogether
Disease index calculates drug effect.
Table 2 prevents and treats powdery mildew of cucumber pharmacy effect comparison sheet
(data source was in field control effectiveness test in 2018)
From table 2 it can be seen that field control effectiveness test result shows under the experimental condition of embodiment 2, jinggangmeisu and spy
Base draws bacillus composition to have excellent preventive effect to powdery mildew of cucumber, and control efficiency has synergistic effect compared with single dose,
The usage amount that can reduce pesticide, reduces cost.
Embodiment 3
Jinggangmeisu and Te Jila bacillus (living bacteria count of Te Jila bacillus is in 100,000,000,000 cfu/g) are pressed
Weight ratio 5:1 compounding, carries out the field trial of powdery mildew of cucumber prevention and treatment, and different agents are made into corresponding dense by the requirement of according to the form below
Degree is administered for the first time in cucumber period, is administered for second after 7 days, and application twice, 7 days after application, 14 days, measures for 21 days altogether
Disease index calculates drug effect.
Table 3 prevents and treats powdery mildew of cucumber pharmacy effect comparison sheet
(data source was in field control effectiveness test in 2018)
From table 3 it can be seen that field control effectiveness test result shows under the experimental condition of embodiment 3, jinggangmeisu and spy
Base draws bacillus composition to have excellent preventive effect to powdery mildew of cucumber, and control efficiency has synergistic effect compared with single dose,
The usage amount that can reduce pesticide, reduces cost.
Embodiment 4
Jinggangmeisu and Te Jila bacillus (living bacteria count of Te Jila bacillus is in 100,000,000,000 cfu/g) are pressed
Weight ratio 3:1 compounding, carries out the field trial of powdery mildew of cucumber prevention and treatment, and different agents are made into corresponding dense by the requirement of according to the form below
Degree is administered for the first time in cucumber period, is administered for second after 7 days, and application twice, 7 days after application, 14 days, measures for 21 days altogether
Disease index calculates drug effect.
Table 4 prevents and treats powdery mildew of cucumber pharmacy effect comparison sheet
(data source was in field control effectiveness test in 2018)
From table 4, it can be seen that field control effectiveness test result shows under the experimental condition of embodiment 4, jinggangmeisu and spy
Base draws bacillus composition to have excellent preventive effect to powdery mildew of cucumber, and control efficiency has synergistic effect compared with single dose,
The usage amount that can reduce pesticide, reduces cost.
Embodiment 5
Jinggangmeisu and Te Jila bacillus (living bacteria count of Te Jila bacillus is in 100,000,000,000 cfu/g) are pressed
Weight ratio 1:1 compounding, carries out the field trial of powdery mildew of cucumber prevention and treatment, and different agents are made into corresponding dense by the requirement of according to the form below
Degree is administered for the first time in cucumber period, is administered for second after 7 days, and application twice, 7 days after application, 14 days, measures for 21 days altogether
Disease index calculates drug effect.
Table 5 prevents and treats powdery mildew of cucumber pharmacy effect comparison sheet
(data source was in field control effectiveness test in 2018)
As can be seen from Table 5, field control effectiveness test result shows under the experimental condition of embodiment 5, jinggangmeisu and spy
Base draws bacillus composition to have excellent preventive effect to powdery mildew of cucumber, and control efficiency has synergistic effect compared with single dose,
The usage amount that can reduce pesticide, reduces cost.
Embodiment 6
Jinggangmeisu and Te Jila bacillus (living bacteria count of Te Jila bacillus is in 100,000,000,000 cfu/g) are pressed
Weight ratio 1:4 compounding, carries out the field trial of powdery mildew of cucumber prevention and treatment, and different agents are made into corresponding dense by the requirement of according to the form below
Degree is administered for the first time in cucumber period, is administered for second after 7 days, and application twice, 7 days after application, 14 days, measures for 21 days altogether
Disease index calculates drug effect.
Table 6 prevents and treats powdery mildew of cucumber pharmacy effect comparison sheet
(data source was in field control effectiveness test in 2018)
As can be seen from Table 6, field control effectiveness test result shows under the experimental condition of embodiment 6, jinggangmeisu and spy
Base draws bacillus composition to have excellent preventive effect to powdery mildew of cucumber, and control efficiency has synergistic effect compared with single dose,
The usage amount that can reduce pesticide, reduces cost.
Embodiment 7
Jinggangmeisu and Te Jila bacillus (living bacteria count of Te Jila bacillus is in 100,000,000,000 cfu/g) are pressed
Weight ratio 1:8 compounding, carries out the field trial of powdery mildew of cucumber prevention and treatment, and different agents are made into corresponding dense by the requirement of according to the form below
Degree is administered for the first time in cucumber period, is administered for second after 7 days, and application twice, 7 days after application, 14 days, measures for 21 days altogether
Disease index calculates drug effect.
Table 7 prevents and treats powdery mildew of cucumber pharmacy effect comparison sheet
(data source was in field control effectiveness test in 2018)
As can be seen from Table 7, field control effectiveness test result shows under the experimental condition of embodiment 7, jinggangmeisu and spy
Base draws bacillus composition to have excellent preventive effect to powdery mildew of cucumber, and control efficiency has synergistic effect compared with single dose,
The usage amount that can reduce pesticide, reduces cost.
Embodiment 8
Jinggangmeisu and Te Jila bacillus (living bacteria count of Te Jila bacillus is in 100,000,000,000 cfu/g) are pressed
Weight ratio 1:15 compounding, carries out the field trial of powdery mildew of cucumber prevention and treatment, and different agents are made into corresponding dense by the requirement of according to the form below
Degree is administered for the first time in cucumber period, is administered for second after 7 days, and application twice, 7 days after application, 14 days, measures for 21 days altogether
Disease index calculates drug effect.
Table 8 prevents and treats powdery mildew of cucumber pharmacy effect comparison sheet
(data source was in field control effectiveness test in 2018)
As can be seen from Table 8, field control effectiveness test result shows under the experimental condition of embodiment 8, jinggangmeisu and spy
Base draws bacillus composition to have excellent preventive effect to powdery mildew of cucumber, and control efficiency has synergistic effect compared with single dose,
The usage amount that can reduce pesticide, reduces cost.
Embodiment 9
Indoor toxicity test of the composite jinggangmycin to watermelon blight
Wherein, watermelon blight germ is acquired by field, is cultivated through indoor purifying.
Test method: after prerun determines the effective inhibition concentration range of each medicament, by Te Jila bacillus (Te Jila bud
The living bacteria count of spore bacillus is in 100,000,000,000 cfu/g), jinggangmeisu and its mixture preparation it is dilute by active constituent content gradient series
5 concentration are released, is compared with sterile water, is measured using mycelial growth rate method, sterilizes culture dish is added in various concentration medicament
In, every ware 1mL adds the PDA culture medium 9mL that sterilizing is melted, mixes well and plate is made, then the bacterium colony to spread cultivation is reached
The bacteria cake of 0.5cm, every ware is a piece of, and 3 repetitions of every processing are placed in 28 DEG C of insulating boxs and cultivate, cultivate 4 days measurement colony diameters,
Mycelial growth inhibition rate is calculated, virulence regression equation is found out, calculates EC50, mycelial growth inhibition rate formula is as follows:
Mycelial growth inhibition rate (%)=(blank control colony diameter-chemicals treatment colony diameter)/blank control bacterium colony
Diameter * 100
The synergistic effect of pharmacy mix is evaluated with the co-toxicity coefficient method (CTC) of Sun Yunpei, i.e. CTC≤80 are antagonism work
With 80 < CTC < 120 are summation action, and CTC≤120 are synergistic effect.
9 jinggangmeisu of table compounds the indoor toxicity test table to watermelon blight with Te Jila bacillus
As can be seen from Table 1, jinggangmeisu, EC of the Te Jila bacillus to watermelon blight50Respectively 20.38mg/L
And 36.09mg/L, when jinggangmeisu is 15:1~1:15 with Te Jila bacillus mass ratio, co-toxicity coefficient is greater than 120, table
Reveal synergistic effect, when jinggangmeisu is 1:1 with Te Jila bacillus mass ratio, co-toxicity coefficient is maximum, and synergistic effect is most
It is good.
Embodiment 10
Jinggangmeisu and Te Jila bacillus (living bacteria count of Te Jila bacillus is in 100,000,000,000 cfu/g) are pressed
Weight ratio 15:1 compounding, carries out the field trial of watermelon blight prevention and treatment, and different agents are made into corresponding dense by the requirement of according to the form below
Degree is administered for the first time in watermelon period, is administered for second after 7 days, and application twice, 7 days after application, 14 days, measures for 21 days altogether
Disease index calculates drug effect.
Table 10 prevents and treats watermelon blight pharmacy effect comparison sheet
(data source was in field control effectiveness test in 2018)
As can be seen from Table 10, field control effectiveness test result shows under the experimental condition of embodiment 10, jinggangmeisu with
Te Jila bacillus composition has excellent preventive effect to watermelon blight, and control efficiency compared with single dose there is synergy to make
With can reduce the usage amount of pesticide, reduce cost.
Embodiment 11
Jinggangmeisu and Te Jila bacillus (living bacteria count of Te Jila bacillus is in 100,000,000,000 cfu/g) are pressed
Weight ratio 5:1 compounding, carries out the field trial of watermelon blight prevention and treatment, and different agents are made into corresponding dense by the requirement of according to the form below
Degree is administered for the first time in watermelon period, is administered for second after 7 days, and application twice, 7 days after application, 14 days, measures for 21 days altogether
Disease index calculates drug effect.
Table 11 prevents and treats watermelon blight pharmacy effect comparison sheet
(data source was in field control effectiveness test in 2018)
As can be seen from Table 11, field control effectiveness test result shows under the experimental condition of embodiment 11, jinggangmeisu with
Te Jila bacillus composition has excellent preventive effect to watermelon blight, and control efficiency compared with single dose there is synergy to make
With can reduce the usage amount of pesticide, reduce cost.
Embodiment 12
Jinggangmeisu and Te Jila bacillus (living bacteria count of Te Jila bacillus is in 100,000,000,000 cfu/g) are pressed
Weight ratio 3:1 compounding, carries out the field trial of watermelon blight prevention and treatment, and different agents are made into corresponding dense by the requirement of according to the form below
Degree is administered for the first time in watermelon period, is administered for second after 7 days, and application twice, 7 days after application, 14 days, measures for 21 days altogether
Disease index calculates drug effect.
Table 12 prevents and treats watermelon blight pharmacy effect comparison sheet
(data source was in field control effectiveness test in 2018)
As can be seen from Table 12, field control effectiveness test result shows under the experimental condition of embodiment 12, jinggangmeisu with
Te Jila bacillus composition has excellent preventive effect to watermelon blight, and control efficiency compared with single dose there is synergy to make
With can reduce the usage amount of pesticide, reduce cost.
Embodiment 13
Jinggangmeisu and Te Jila bacillus (living bacteria count of Te Jila bacillus is in 100,000,000,000 cfu/g) are pressed
Weight ratio 1:1 compounding, carries out the field trial of watermelon blight prevention and treatment, and different agents are made into corresponding dense by the requirement of according to the form below
Degree is administered for the first time in watermelon period, is administered for second after 7 days, and application twice, 7 days after application, 14 days, measures for 21 days altogether
Disease index calculates drug effect.
Table 13 prevents and treats watermelon blight pharmacy effect comparison sheet
(data source was in field control effectiveness test in 2018)
As can be seen from Table 13, field control effectiveness test result shows under the experimental condition of embodiment 13, jinggangmeisu with
Te Jila bacillus composition has excellent preventive effect to watermelon blight, and control efficiency compared with single dose there is synergy to make
With can reduce the usage amount of pesticide, reduce cost.
Embodiment 14
Jinggangmeisu and Te Jila bacillus (living bacteria count of Te Jila bacillus is in 100,000,000,000 cfu/g) are pressed
Weight ratio 1:4 compounding, carries out the field trial of watermelon blight prevention and treatment, and different agents are made into corresponding dense by the requirement of according to the form below
Degree is administered for the first time in watermelon period, is administered for second after 7 days, and application twice, 7 days after application, 14 days, measures for 21 days altogether
Disease index calculates drug effect.
Table 14 prevents and treats watermelon blight pharmacy effect comparison sheet
(data source was in field control effectiveness test in 2018)
As can be seen from Table 14, field control effectiveness test result shows under the experimental condition of embodiment 14, jinggangmeisu with
Te Jila bacillus composition has excellent preventive effect to watermelon blight, and control efficiency compared with single dose there is synergy to make
With can reduce the usage amount of pesticide, reduce cost.
Embodiment 15
Jinggangmeisu and Te Jila bacillus (living bacteria count of Te Jila bacillus is in 100,000,000,000 cfu/g) are pressed
Weight ratio 1:8 compounding, carries out the field trial of watermelon blight prevention and treatment, and different agents are made into corresponding dense by the requirement of according to the form below
Degree is administered for the first time in watermelon period, is administered for second after 7 days, and application twice, 7 days after application, 14 days, measures for 21 days altogether
Disease index calculates drug effect.
Table 15 prevents and treats watermelon blight pharmacy effect comparison sheet
(data source was in field control effectiveness test in 2018)
As can be seen from Table 15, field control effectiveness test result shows under the experimental condition of embodiment 15, jinggangmeisu with
Te Jila bacillus composition has excellent preventive effect to watermelon blight, and control efficiency compared with single dose there is synergy to make
With can reduce the usage amount of pesticide, reduce cost.
Embodiment 16
Jinggangmeisu and Te Jila bacillus (living bacteria count of Te Jila bacillus is in 100,000,000,000 cfu/g) are pressed
Weight ratio 1:15 compounding, carries out the field trial of watermelon blight prevention and treatment, and different agents are made into corresponding dense by the requirement of according to the form below
Degree is administered for the first time in watermelon period, is administered for second after 7 days, and application twice, 7 days after application, 14 days, measures for 21 days altogether
Disease index calculates drug effect.
Table 16 prevents and treats watermelon blight pharmacy effect comparison sheet
(data source was in field control effectiveness test in 2018)
As can be seen from Table 16, field control effectiveness test result shows under the experimental condition of embodiment 16, jinggangmeisu with
Te Jila bacillus composition has excellent preventive effect to watermelon blight, and control efficiency compared with single dose there is synergy to make
With can reduce the usage amount of pesticide, reduce cost.
By above embodiments, it can be concluded that, the present invention will be synergistic bright after jinggangmeisu and Te Jila bacillus mixture
It is aobvious, drug effect can be increased substantially, Pesticide use amount and drug cost are reduced.Meanwhile bactericidal composition provided by the invention can mention
The lasting period of high jinggangmeisu.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of bactericidal composition containing jinggangmeisu, which is characterized in that the compound includes jinggangmeisu and Te Jila bud
Spore bacillus；
The jinggangmeisu and the mass ratio of Te Jila bacillus are (1~15): (1~15)；
The living bacteria count of the Te Jila bacillus is in 100,000,000,000 cfu/g or more.
2. bactericidal composition according to claim 1, which is characterized in that the jinggangmeisu and Te Jila bacillus
Mass ratio is (1.5~5): (1.5~8).
3. application of the bactericidal composition of any of claims 1 or 2 in controlling plant diseases.
4. application according to claim 3, which is characterized in that the plant disease includes powdery mildew and/or wilt disease.
5. application according to claim 3, which is characterized in that the plant includes cucumber and/or watermelon.
6. Bactericidal composite group of any of claims 1 or 2 is preparing the application in pesticide.
7. application according to claim 6, which is characterized in that the dosage form of the pesticide includes aqueous suspension agent and/or wettable
Property pulvis.
8. application according to claim 6, which is characterized in that mass percentage of the jinggangmeisu in pesticide be
4~30%.
9. application according to claim 6, which is characterized in that content of the Te Jila bacillus in pesticide is 5
~20 hundred million cfu/g.
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| CN201910773214.5A Pending CN110402929A (en) | 2019-08-21 | 2019-08-21 | A kind of bactericidal composition and its application containing jinggangmeisu |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112479780A (en) * | 2020-12-18 | 2021-03-12 | 西南林业大学 | Special biological bacterial fertilizer for panax notoginseng under forest |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104403962A (en) * | 2014-10-31 | 2015-03-11 | 中国农业科学院农产品加工研究所 | Bacillus tequilensis and its use in prevention and control of fusarium graminearum |
| CN105695368A (en) * | 2016-04-11 | 2016-06-22 | 江苏省农业科学院 | Tequila bacillus and application thereof |
| CN108651524A (en) * | 2018-05-08 | 2018-10-16 | 武汉楚强生物科技有限公司 | The complex preparation and its preparation of a kind of jinggangmeisu and bacillus subtilis and application |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104403962A (en) * | 2014-10-31 | 2015-03-11 | 中国农业科学院农产品加工研究所 | Bacillus tequilensis and its use in prevention and control of fusarium graminearum |
| CN105695368A (en) * | 2016-04-11 | 2016-06-22 | 江苏省农业科学院 | Tequila bacillus and application thereof |
| CN108651524A (en) * | 2018-05-08 | 2018-10-16 | 武汉楚强生物科技有限公司 | The complex preparation and its preparation of a kind of jinggangmeisu and bacillus subtilis and application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112479780A (en) * | 2020-12-18 | 2021-03-12 | 西南林业大学 | Special biological bacterial fertilizer for panax notoginseng under forest |
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