CN117918368A - Use of bactericidal composition containing benzovindiflupyr and pyraclostrobin for controlling plant anthracnose diseases - Google Patents
Use of bactericidal composition containing benzovindiflupyr and pyraclostrobin for controlling plant anthracnose diseases Download PDFInfo
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- CN117918368A CN117918368A CN202410085916.5A CN202410085916A CN117918368A CN 117918368 A CN117918368 A CN 117918368A CN 202410085916 A CN202410085916 A CN 202410085916A CN 117918368 A CN117918368 A CN 117918368A
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- pyraclostrobin
- benzovindiflupyr
- colletotrichum
- anthracnose
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- 239000005869 Pyraclostrobin Substances 0.000 title claims abstract description 59
- 239000005737 Benzovindiflupyr Substances 0.000 title claims abstract description 58
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- HZRSNVGNWUDEFX-UHFFFAOYSA-N pyraclostrobin Chemical compound COC(=O)N(OC)C1=CC=CC=C1COC1=NN(C=2C=CC(Cl)=CC=2)C=C1 HZRSNVGNWUDEFX-UHFFFAOYSA-N 0.000 title claims abstract description 58
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Abstract
The invention belongs to the field of pesticide bactericides, and discloses application of a bactericidal composition containing benzovindiflupyr and pyraclostrobin in preventing and controlling plant anthracnose diseases; the active ingredients of the bactericidal composition comprise active ingredient A benzovindiflupyr and active ingredient B pyraclostrobin. The bactericidal composition can be used for preventing and treating diseases caused by anthrax on plants, has good preventing and treating effect, and has the characteristics of wide bactericidal spectrum, low dosage, obvious synergistic effect and the like.
Description
Technical Field
The invention relates to the field of pesticide bactericides, in particular to application of a bactericidal composition containing benzovindiflupyr and pyraclostrobin in preventing and controlling plant anthracnose.
Background
The benzovindiflupyr is a pyrazole amide fungicide developed by the first da, is a succinic acid dehydrogenase inhibitor, is widely used for preventing and treating various diseases such as wheat powdery mildew, asian soybean rust and the like, has no cross resistance with methoxy acrylic acid esters and triazole fungicides, and can be compounded with various fungicides. The chemical structural formula is as follows:
Pyraclostrobin is methoxy acrylic bactericide, mitochondrial respiration inhibitor, and has effects of protecting, treating, and blade penetrating and conducting. The bactericide has direct effect on various pathogenic bacteria, and is beneficial to crop growth and yield improvement.
The prior art CN108094433A discloses a bactericidal composition, and particularly discloses that benzovindiflupyr and pyraclostrobin have good synergism on rice sheath blight within the range of 1:30-30:1; CN104839165A discloses a bactericidal composition of benzovindiflupyr, and also discloses the application of the bactericidal composition of benzovindiflupyr to the prevention and treatment of crops, wherein the mass ratio of the benzovindiflupyr to the pyraclostrobin is 1:20-20:1. However, no report is available on the application of benzovindiflupyr and pyraclostrobin in preventing and controlling plant anthracnose.
Disclosure of Invention
Based on the above circumstances, the invention aims to provide the application of the bactericidal composition containing benzovindiflupyr and pyraclostrobin in preventing and controlling plant anthracnose diseases, and the bactericidal composition has the characteristics of good prevention and control effect, wide bactericidal spectrum, low dosage, obvious synergistic effect and the like.
In order to achieve the above object, the following technical scheme is provided: the application of a bactericidal composition containing benzovindiflupyr and pyraclostrobin in preventing and controlling plant anthracnose diseases is disclosed, wherein the active ingredients of the bactericidal composition comprise active ingredient A benzovindiflupyr and active ingredient B pyraclostrobin, and the mass ratio of the benzovindiflupyr to the pyraclostrobin is 1:35-25:1;
Further, the mass ratio of the active ingredients of benzovindiflupyr to pyraclostrobin is 1:24-25:1;
Further, the mass ratio of the active ingredients of benzovindiflupyr to pyraclostrobin is 1:10-14:1;
further, based on 100 weight percent of the total weight of the sterilization composition, the sum of the contents of the benzovindiflupyr and the pyraclostrobin in the sterilization composition is 5-90 weight percent;
further, the sum of the contents of the benzovindiflupyr and the pyraclostrobin in the bactericidal composition is 5-80 wt%;
further, the dosage form of the bactericidal composition can be any one of emulsifiable concentrate, aqueous emulsion, microemulsion, suspending agent, wettable powder or water dispersible granule;
further, the plant anthracnose is anthracnose caused by Colletotrichum (Colletotrichum) fungi;
Further, the anthrax (Colletotrichum) fungus includes Colletotrichum gloeosporioides [ Colletotrichumhigginsianum ], curbitaceae, colletotrichum [ Colletotrichum orbiculare ], colletotrichum [ Colletotrichumlindemuthianum ], colletotrichum capsici [ Colletotrichum capsici ], colletotrichum [ Colletotrichumgloeosporioides (penz.) penz et Sacc ];
further, the plant diseases caused by the anthracnose are cucumber anthracnose [ Colletotrichumorbiculare ], citrus anthracnose [ Colletotrichumgloeosporioides (penz.) penz et Sacc ];
further, the bactericidal composition is applied to diseases or growth media thereof to be controlled in an effective dose.
Compared with the prior art, the technical scheme of the invention has the following beneficial effects:
1) The benzovindiflupyr and pyraclostrobin are compounded, so that the bactericidal activity is increased, and the plant anthracnose diseases can be effectively prevented and treated;
2) The pesticide is safe and environment-friendly, has wide development prospect, reduces the use amount of the pesticide and reduces the pesticide cost of farmers;
3) Has the advantages of super high efficiency, broad bactericidal spectrum, and can delay the generation of drug resistance of harmful bacteria and prolong the duration of the drug.
Detailed Description
The present invention will be described in more detail with reference to the following examples, but the present invention can be embodied in various forms and should not be construed as being limited to the embodiments set forth herein.
Indoor toxicity measurement
Example 1
Indoor combined action test of benzovindiflupyr and pyraclostrobin on cucumber anthracnose
The test is based on: test reference NY/T1156.2-2006 section 2 of pesticide indoor bioassay test criteria section 2: test plate method for inhibiting growth of pathogenic fungi; NY/T1156.6-2006, determination of the combined action of the pesticide according to section 6 of the pesticide according to the criterion of biological assay in pesticide room.
Test agent: 96% of benzovindiflupyr original drug and 97% of pyraclostrobin original drug are provided by a group development center.
Test pathogenic bacteria: cucumber colletotrichum (Colletotrichum lagenarium (pass.) ell.et halst).
And (3) preparation of a medicament: the test stock was dissolved in acetone and then diluted with 0.1% tween 80 in water. Preparing single-dose mother liquor respectively, setting 5 series of mass concentrations according to the mixing purpose and the medicament activity, and controlling the final content of the organic solvent to be not more than 2%.
Melting PDA culture medium with microwave oven, cooling to 50deg.C, taking 1mL of prepared medicinal liquid to be tested and 9mL of PDA culture medium according to the principle from low concentration to high concentration, adding into culture dish with diameter of 9cm, mixing, and making into tablet with corresponding concentration.
Inoculating: and (3) beating the cultured pathogenic fungi into fungus cakes by using a puncher with the diameter of 6mm under the aseptic condition, placing the fungus cakes in the central position of a culture medium after the drug-containing culture medium is solidified, sealing a culture dish by using a sealing film, culturing in an incubator at the temperature of 27 ℃, and setting a blank solution without the drug as a blank control, wherein each treatment is repeated for 4 times.
Investigation: after 12d incubation, colony diameters were measured in millimeters (mm) using calipers. The diameter of each colony was measured vertically by the cross-over method and the average value was taken.
Data statistics and analysis: the growth of pathogenic hyphae was investigated according to the growth of bacteria in a blank culture dish. Colony diameter was measured using the crisscross method.
According to the investigation result, the hypha growth inhibition rate of each treatment concentration to the target bacteria to be tested is calculated, the unit is percentage (%), and the calculation result is reserved for two positions after decimal point.
D＝D1-D2
Wherein:
D-colony growth diameter;
d 1 —colony diameter;
D 2 -diameter of the fungus cake.
I, hypha growth inhibition rate;
d 0 —a control colony growth diameter;
d t —agent treated colony growth diameter.
And (3) test statistics: and processing the data by adopting a probability value analysis method. The toxicity regression line, R, EC 50 value (95% confidence interval) and EC 90 value (95% confidence interval) are obtained by using DPS statistical analysis system to evaluate the activity of the test agent on the biological test material.
The co-toxicity coefficient (CTC value) of the blend was calculated as follows:
Wherein:
Ati—actual measured virulence index of the mixture;
S-EC 50 of standard bactericide in milligrams per liter (mg/L);
M-EC 50 of the mixture in milligrams per liter (mg/L).
TTI＝TIA*PA+TIB*PB
Wherein:
TTI-the theoretical toxicity index of the mixture;
TI A A agent toxicity index;
The percentage content of the P A -A medicament in the mixture is expressed as percentage (%);
TI B -agent toxicity index;
The percentage of the P B -B medicament in the mixture is expressed as percentage (%).
Wherein:
Ctc—co-toxicity coefficient;
Ati—actual measured virulence index of the mixture;
TTI-the theoretical toxicity index of the mixture.
The compound co-toxicity coefficient CTC is more than or equal to 120 and shows synergistic effect; ctc.ltoreq.80 shows antagonism; 80 < CTC < 120 shows additive effect.
The test results are shown in the following table:
As shown in an indoor activity test (see Table 1), the benzovindiflupyr and pyraclostrobin are mixed to be 0.765mg/L and 0.1530mg/L respectively for cucumber anthracnose bacteria EC 50, and the cucumber anthracnose bacteria are sensitive to pyraclostrobin; the co-toxicity coefficients of the benzovindiflupyr and the pyraclostrobin are all larger than 80 after being mixed according to the proportion of 1:35-25:1, and the benzovindiflupyr and the pyraclostrobin show additive or synergistic effect on preventing and controlling cucumber anthracnose; the co-toxicity coefficients of the benzovindiflupyr and the pyraclostrobin are all larger than 120 after being mixed according to the proportion of 1:24-25:1, and the synergistic effect is shown for preventing and controlling cucumber anthracnose; the co-toxicity coefficient of the benzovindiflupyr and the pyraclostrobin is the largest after being mixed according to the ratio of 3:1, and is 173.08, and the EC 50 of the benzovindiflupyr and the pyraclostrobin is 0.221mg/L.
TABLE 1 indoor combined action test results of benzovindiflupyr and pyraclostrobin on cucumber anthracnose
Example 2
Indoor combined action test of benzovindiflupyr and pyraclostrobin on citrus anthracnose
The test is based on: test reference NY/T1156.2-2006 section 2 of pesticide indoor bioassay test criteria section 2: test plate method for inhibiting growth of pathogenic fungi; NY/T1156.6-2006, determination of the combined action of the pesticide according to section 6 of the pesticide according to the criterion of biological assay in pesticide room.
Test strain: citrus anthracnose germ (Colletotrichum gloeosporioides).
Test agent: 96% of benzovindiflupyr original drug and 97% of pyraclostrobin original drug are provided by a group development center.
And (3) preparation of a medicament: the raw materials are dissolved by acetone, single-dose mother solutions are respectively prepared, different proportions are designed according to the mixing purpose and the medicament activity, and each single dose and each group of proportion mixture are prepared into a series of mass concentration gradients.
Under aseptic operation conditions, the sterilized PDA culture medium melted in advance is cooled to 50 ℃, 1mL of the liquid medicine and 9mL of the PDA culture medium are respectively sucked by a liquid transfer device and are uniformly mixed in a culture dish with the diameter of 9cm, and a flat culture medium with medicine is prepared, and sterile water is used as a blank control. Each treatment was repeated 4 times.
Inoculating: activating the stored pathogenic bacteria, cutting bacterial cakes from the edge of bacterial colony under aseptic operation condition by using a sterilizing puncher with the diameter of 6mm, inoculating the bacterial cakes to the center of a medicine-containing flat plate culture medium by using an inoculator, placing 1 bacterial cake in each culture dish with the mycelium surface facing upwards, covering a dish cover, and culturing in a constant temperature incubator at 25 ℃.
Data statistics and analysis: the growth of pathogenic hyphae was investigated according to the growth of bacteria in a blank culture dish. Colony diameter was measured using the crisscross method.
According to the investigation result, the hypha growth inhibition rate of each treatment concentration to the target bacteria to be tested is calculated, the unit is percentage (%), and the calculation result is reserved for two positions after decimal point.
D＝D1-D2
Wherein:
D-colony growth diameter;
d 1 —colony diameter;
D 2 -diameter of the fungus cake.
I, hypha growth inhibition rate;
d 0 —a control colony growth diameter;
d t —agent treated colony growth diameter.
And (3) test statistics: and processing the data by adopting a probability value analysis method. The toxicity regression line, R, EC 50 value (95% confidence interval) and EC 90 value (95% confidence interval) are obtained by using DPS statistical analysis system to evaluate the activity of the test agent on the biological test material.
The co-toxicity coefficient (CTC value) of the blend was calculated as follows:
Wherein:
Ati—actual measured virulence index of the mixture;
S-EC 50 of standard bactericide in milligrams per liter (mg/L);
M-EC 50 of the mixture in milligrams per liter (mg/L).
TTI＝TIA*PA+TIB*PB
Wherein:
TTI-the theoretical toxicity index of the mixture;
TI A A agent toxicity index;
The percentage content of the P A -A medicament in the mixture is expressed as percentage (%);
TI B -agent toxicity index;
The percentage of the P B -B medicament in the mixture is expressed as percentage (%).
Wherein:
Ctc—co-toxicity coefficient;
Ati—actual measured virulence index of the mixture;
TTI-the theoretical toxicity index of the mixture.
The compound co-toxicity coefficient CTC is more than or equal to 120 and shows synergistic effect; ctc.ltoreq.80 shows antagonism; 80 < CTC < 120 shows additive effect.
The test results are shown in the following table:
as shown in an indoor activity test (see Table 2), the benzovindiflupyr and pyraclostrobin are mixed to be 0.812mg/L and 0.145mg/L respectively for citrus anthracnose bacteria EC 50, and the citrus anthracnose bacteria are sensitive to pyraclostrobin; the co-toxicity coefficients of the benzovindiflupyr and the pyraclostrobin are all larger than 80 after being mixed according to the proportion of 1:30-25:1, and the benzovindiflupyr and the pyraclostrobin show additive or synergistic effect on the prevention and treatment of citrus anthracnose; the co-toxicity coefficients of the benzovindiflupyr and the pyraclostrobin are all larger than 120 after being mixed according to the proportion of 1:10-14:1, and the benzovindiflupyr and the pyraclostrobin show synergistic effect on preventing and controlling citrus anthracnose; the biggest co-toxicity coefficient of benzovindiflupyr and pyraclostrobin after being mixed according to the ratio of 7:2 is 139.91, and the EC 50 of the benzovindiflupyr and pyraclostrobin is 0.287mg/L.
TABLE 2 indoor combined action test results of benzovindiflupyr and pyraclostrobin on citrus anthracnose
Specific preparation examples:
preparation example 1:
20% Benzenofloxacin pyraclostrobin suspension (15:5)
The formula comprises the following components: 15% of benzovindiflupyr, 5% of pyraclostrobin, 0.5% of sodium dodecyl sulfate, 1.2% of sodium lignin sulfonate, 2.5% of polyether, 2.5% of phenethyl phenol polyether phosphate, 1.5% of guerbet alcohol polyoxyethylene ether (xp-70), 1% of magnesium aluminum silicate, 0.1% of xanthan gum, 5% of ethylene glycol, 0.02% of benzisothiazolinone, 0.4% of simethicone and deionized water;
the preparation method comprises the following steps: adding benzovindiflupyr and an auxiliary agent (a preservative and a thickening agent) into a feeding kettle, and starting shearing to completely dissolve the auxiliary agent. Adding pyraclostrobin under high-shear stirring, shearing uniformly, sanding, transferring into a homogenizing kettle, adding preservative and thickener, adding residual water to make up the balance, shearing, and homogenizing and mixing to obtain corresponding product.
Preparation example 2:
32% benzovindiflupyr pyraclostrobin suspension (20:12)
The formula comprises the following components: 20% of benzovindiflupyr, 12% of pyraclostrobin, 2.5% of polycarboxylate, 3% of phenethyl phenol polyether phosphate, 2% of isomeric tridecanol polyoxyethylene ether, 1% of magnesium aluminum silicate, 0.2% of xanthan gum, 5% of propylene glycol, 0.1% of benzoic acid, 0.35% of simethicone and the balance of deionized water;
the preparation method comprises the following steps: the same as in preparation example 1.
Preparation example 3:
50% benzovindiflupyr-pyraclostrobin water dispersible granule (36:14)
The formula comprises the following components: 36% of benzovindiflupyr, 14% of pyraclostrobin, 5% of lignosulfonate, 7.5% of sodium dodecyl benzene sulfonate, 8% of aluminum chloride and the balance of white carbon black;
The preparation method comprises the following steps: according to the formula proportion of the embodiment, the active ingredients of benzovindiflupyr and pyraclostrobin are added into a carrier, a surfactant and other functional auxiliary agents are added into the carrier, the mixture is mixed, 10-25% of water is added after jet milling, and then the water dispersible granule product is prepared through kneading, granulating, drying and screening.
Example 3
Benzovindiflupyr and pyraclostrobin compounded field efficacy test for cucumber anthracnose
The test is based on: the test is described in GB/T17980.112-2004 section 112 of pesticide field efficacy test criterion (two): the bactericide can prevent and treat anthracnose of melons.
Test crop: cucumber (jin Chun No. 2).
Test site: the open field cucumber field in the Laixi market has cucumber as the previous crop in the test field, medium soil fertility, field planting density of 5 ten thousand plants/hm 2 and uniform plant growth vigor.
And (3) test design: the random block arrangement of each cell was tested, the cell area was 30m 2, and each cell was repeated 4 times.
Time of application: the cucumber plants are uniformly sprayed by adopting a industrial and agricultural 16-type knapsack sprayer, and the pesticide application liquid amount is 45 kg/mu. The test was performed for a first time in month 8 of 2022, followed by 1 more administration at 7-day intervals for a total of two administrations.
Experimental investigation: disease number was investigated before the second application (i.e. 7d after the first application) and disease index was investigated after the second application and 11d after the second application and control effect was calculated for a total of 3 surveys.
The investigation method comprises the following steps: 5-point sampling method for each cell, 3 plants are investigated for each point, and 5-10 leaves are investigated for each plant from top to bottom.
The classification was performed according to the following classification method:
Level 0: no disease spots;
Stage 1: the area of the disease spots accounts for less than 5% of the whole leaf area;
3 stages: the area of the lesion accounts for 6-10% of the whole leaf area;
5 stages: the area of the lesion accounts for 11% -25% of the whole leaf area;
7 stages: the area of the lesion accounts for 26% -50% of the whole leaf area;
7 stages: the area of the disease spots accounts for more than 51% of the whole leaf area;
The drug effect calculation method comprises the following steps:
The cucumber growth in each treatment cell is observed to be good during the test period, and each treatment agent does not generate chemical injury to cucumber plants and other non-target organisms at the tested concentration.
The results of the field efficacy test are shown in the following table:
TABLE 3 results of field efficacy test of benzovindiflupyr and pyraclostrobin on cucumber anthracnose
Field efficacy display (see table 3): under the same environmental conditions, the overall control effect of the three different treatments for controlling cucumber anthracnose is 69.48-83.21% in each treatment field effect 7 days after the first application, and the control effects of the three different doses of 80g/hm 2、100g/hm2、120g/hm2 of the 50% benzovindiflupyr wettable powder on cucumber anthracnose are respectively 81.09%, 82.72% and 83.21% different from those of the control agents (45% benzovindiflupyr-azoxystrobin water dispersible granule (30+15), 9% benzovindiflupyr emulsifiable concentrate and 25% pyraclostrobin suspending agent).
The overall prevention effect of each treatment field effect is 71.79-85.63% after the second application, and the prevention effect of 50% benzovindiflupyr after the three different doses of 80g/hm 2、100g/hm2、120g/hm2 of the pyraclostrobin wettable powder on cucumber anthracnose is 83.15%, 84.21% and 85.63% respectively.
Through indoor toxicity measurement and field experiments, the pesticide composition compounded by the benzovindiflupyr and the pyraclostrobin has a good control effect on plant anthracnose diseases.
The pesticide composition or the preparation prepared by the combination has remarkable prevention effect, and is superior to a single dose in the aspects of delaying the generation of drug resistance and prolonging the lasting period. In addition, no phytotoxicity of the compound medicament to crops is found in the test, which proves that the production cost and the use cost can be reduced and the pesticide composition or the pesticide preparation is safe to crops under the condition that the sterilization synergy of the obtained pesticide composition or the pesticide preparation is improved.
While the invention has been described in detail in terms of the general description and the specific embodiments, it will be apparent to those skilled in the art that various modifications and improvements can be made thereto without departing from the spirit of the invention.
Claims (10)
1. The application of a bactericidal composition containing benzovindiflupyr and pyraclostrobin in controlling plant anthracnose is characterized in that: the bactericidal composition comprises active ingredients A benzovindiflupyr and active ingredients B pyraclostrobin, wherein the mass ratio of the benzovindiflupyr to the pyraclostrobin is 1:35-25:1.
2. Use according to claim 1, characterized in that: the mass ratio of the active ingredients of benzovindiflupyr to pyraclostrobin is 1:24-25:1.
3. Use according to claim 1, characterized in that: the mass ratio of the active ingredients of benzovindiflupyr to pyraclostrobin is 1:10-14:1.
4. Use according to claim 1, characterized in that: based on the total weight of the sterilization composition as 100wt%, the sum of the contents of the benzovindiflupyr and the pyraclostrobin in the sterilization composition is 5-90 wt%.
5. Use according to claim 4, characterized in that: preferably, the sum of the contents of the benzovindiflupyr and the pyraclostrobin in the bactericidal composition is 5-80 wt%.
6. Use according to claim 1, characterized in that: the sterilization composition can be any one of missible oil, aqueous emulsion, microemulsion, suspending agent, wettable powder or water dispersible granule.
7. Use according to claim 1, characterized in that: the plant anthracnose is anthracnose caused by Colletotrichum (Colletotrichum) fungi.
8. Use according to claim 7, characterized in that: the Colletotrichum (Colletotrichum) fungi include Colletotrichum [ Colletotrichum higginsianum ], cucurbitaceae Colletotrichum [ Colletotrichum orbiculare ], bean Colletotrichum [ Colletotrichum lindemuthianum ], pepper Colletotrichum [ Colletotrichumcapsici ], colletotrichum [ Colletotrichumgloeosporioides (penz.) penz et Sacc ].
9. Use according to claim 7, characterized in that: the plant diseases caused by the anthracnose are cucumber anthracnose [ Colletotrichum orbiculare ] and citrus anthracnose [ Colletotrichum gloeosporioides (penz.) penz et Sacc ].
10. The fungicidal composition of claim 1, wherein the fungicidal composition is applied to a disease or a growth medium thereof to be controlled in an effective amount.
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