CN117256615A - Pesticide composition containing polyoxin and application thereof - Google Patents

Pesticide composition containing polyoxin and application thereof Download PDF

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Publication number
CN117256615A
CN117256615A CN202311225752.3A CN202311225752A CN117256615A CN 117256615 A CN117256615 A CN 117256615A CN 202311225752 A CN202311225752 A CN 202311225752A CN 117256615 A CN117256615 A CN 117256615A
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China
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active ingredient
pesticide composition
polyoxin
agent
emulsion
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Inventor
葛家成
李敏
葛晓甜
杨志鹏
王玉
隋书婷
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Qingdao Tengrunxiang Testing And Evaluation Co ltd
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Qingdao Tengrunxiang Testing And Evaluation Co ltd
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Priority to CN202311225752.3A priority Critical patent/CN117256615A/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20—Cereals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00—Fungicides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention belongs to the technical field of pesticide sterilization, and particularly discloses a pesticide composition containing polyoxin, which comprises an active ingredient I and an active ingredient II, wherein the active ingredient I is polyoxin B, the active ingredient II is one of penflufen or bixafen, and the mass ratio of the active ingredient I to the active ingredient II is 1:48-50:1. The pesticide composition has a synergistic effect on various pathogenic bacteria, can effectively reduce the dosage of pesticides, and is safe to crops and other non-target organisms.

Description

Pesticide composition containing polyoxin and application thereof
Technical Field
The invention belongs to the technical field of pesticide sterilization, and particularly relates to a pesticide composition containing polyoxin and application thereof.
Background
Polyoxin B (polyoxin), also called polyoxin, is a metabolite produced by streptomyces aureofaciens, is a peptide pyrimidine nucleoside agricultural antibiotic produced by using modern bioengineering technology, and has systemic and therapeutic effects and high target bioselectivity. The mechanism of action is to inhibit the synthesis of fungal cell wall chitin, the structure of which is similar to that of the substrate uridine diphosphate aminoglucose of chitin synthase, belonging to competitive inhibitors. As it is a safe pesticide with high efficiency, low toxicity and no environmental pollution, it can be widely used for preventing and curing several important diseases of grain crops, fruits and vegetables, etc..
Cyproconazole, ISO common name: sedaxane, CAS number: 874967-67-6, chemical name: n- [2- [1,1' -Bicylopropyl ] -2-ylphenyl ] -3- (difluoromethyl) -1-methyl-1H-pyrazole-carboxamide has a structure which is divided into cis-and trans-structures, with the trans-structure being the main structure. The cyproconazole is a succinic dehydrogenase inhibitor bactericide, and the protein complex II (i.e. succinic dehydrogenase) on the mitochondrial respiratory electron transfer chain of pathogenic bacteria is inhibited to influence the mitochondrial respiratory electron transfer system of the pathogenic bacteria, prevent the energy metabolism of the pathogenic bacteria, inhibit the growth of the pathogenic bacteria and cause the death of the pathogenic bacteria. Is suitable for crops such as grains, soybeans, rape, potatoes, sugarcanes and the like, and can be used for treating seeds to prevent and treat diseases such as smut, head smut, cladosporium cucumerinum and the like.
Bixafen is a bactericide developed by Bayer, and has the chemical name of N- (3 ',4' -dichloro-5-fluoro [1,1' -biphenyl ] -2-yl) -3- (difluoromethyl) -1-methyl-1H-pyrazole-4-carboxamide. Bixafen belongs to a succinate dehydrogenase inhibitor and acts by inhibiting succinate dehydrogenase in mitochondrial respiration. The composition is mainly applied to crops such as cotton, barley, sunflower, soybean, wheat, rape and the like, and can be used for preventing and treating diseases caused by ascomycetes, basidiomycetes and fungi imperfecti. The bixafen has remarkable effects in preventing and treating diseases such as leaf blight, rust disease and the like of cereal crops.
In agricultural production, a large amount of single pesticide is used, so that the target fungus resistance development is caused, the service life of the pesticide is greatly shortened, the use dosage of the pesticide is increased year by year, the negative influence on the environment is increased year by year, and the continuous healthy development of agriculture is not facilitated.
Disclosure of Invention
The invention aims at the problems and provides a pesticide composition containing polyoxin and a preparation thereof, wherein the pesticide composition has good control effect on various plant diseases, can effectively reduce the dosage of pesticides, reduce the cost of pesticides, is safe to crops and reduces the pesticide residue of agricultural products.
In order to achieve the above purpose, the present invention adopts the following technical scheme: the pesticide composition comprises an active ingredient I and an active ingredient II, wherein the active ingredient I is polyoxin B, and the active ingredient II is any one of cyproconazole or bixafen;
further, the active ingredient II is cyproconazole, and the mass ratio of the active ingredient I to the active ingredient II is 1:30-10:1;
further, the mass ratio of the active ingredient I to the active ingredient II is 1:20-10:1;
further, the active ingredient II is bixafen, and the mass ratio of the active ingredient I to the active ingredient II is 1:30-10:1;
further, the mass ratio of the active ingredient I to the active ingredient II is 1:24-10:1;
further, the total weight of the pesticide composition is calculated by 100 weight percent, and the total weight of the active ingredient I and the active ingredient II accounts for 1-90% of the total weight of the pesticide composition;
further, the total weight of the pesticide composition is calculated as 100 weight percent, and the total weight of the active ingredient I and the active ingredient II accounts for 2-80 percent of the total weight of the pesticide composition;
further, the pesticide composition comprises agriculturally acceptable auxiliary ingredients besides the active ingredients, wherein the auxiliary ingredients are selected from one or more of wetting agents, dispersing agents, emulsifying agents, thickening agents, disintegrating agents, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, synergists or carriers;
further, the wetting agent is selected from one or a mixture of more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, nekal BX, wetting penetrating agent F, chinese honeylocust fruit powder, silkworm excrement or soapberry powder;
further, the dispersing agent is selected from one or more of polycarboxylate, lignosulfonate, alkylphenol ethoxylate formaldehyde condensate sulfate, calcium alkylbenzenesulfonate, naphthalene sulfonate formaldehyde condensate sodium salt, alkylphenol ethoxylate, fatty amine ethoxylate, fatty acid ethoxylate or glycerin fatty acid ester ethoxylate;
further, the emulsifier is selected from one or a mixture of more of calcium alkylbenzenesulfonate, OP series phosphate (nonylphenol polyoxyethylene ether phosphate), phenylphenol polyoxyethylene ether phosphate, styrene polyoxyethylene ether ammonium sulfate, alkyl diphenyl ether disulfonate magnesium salt, triethanolamine salt, benzyl dimethyl phenol polyoxyethylene ether, alkylphenol formaldehyde resin polyoxyethylene ether, phenethyl phenol polyoxyethylene polypropylene ether, ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), castor oil polyoxyethylene ether, alkylaryl polyoxyethylene polyoxypropylene ether, sorbitan monostearate, sorbitan fatty acid ester polyoxyethylene ether or fatty alcohol polyoxyethylene ether;
further, the thickener is selected from one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose or magnesium aluminum silicate;
further, the disintegrating agent is selected from one or a mixture of more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate;
further, the antifreezing agent is selected from one or a mixture of more of ethylene glycol, propylene glycol, glycerol or urea;
further, the defoamer is selected from silicone oil, silicone compound, C 10 ～C 20 Saturated fatty acid compounds or C 8 ～C 10 A mixture of one or more fatty alcohol compounds;
further, the solvent is selected from one or more of N, N-dimethylformamide, cyclohexanone, butyl ether, dimethylbenzene, dimethyl sulfoxide, methanol, ethylene glycol, ethanol, propanol, butanol, trimethylcyclohexanone, N-octyl pyrrolidone, toluene, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, diethylene glycol, ethylene glycol methyl ether, ethyl acetate or acetonitrile;
further, the stabilizer is selected from one or a mixture of more of epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate and triphenyl phosphate;
further, the penetrating agent is selected from one or a mixture of more of penetrating agent JFC, penetrating agent T, azone or organic silicon;
further, the carrier is one, two or three of a solvent or a filler, and water is preferably deionized water;
further, the filler is selected from one or a mixture of more of kaolin, diatomite, bentonite, attapulgite, white carbon black, starch or light calcium carbonate;
all of the above are commercially available;
the pesticide composition can be prepared into any one of agriculturally acceptable preparation formulations;
further, the preparation formulation is solid preparation, liquid preparation and/or seed treatment preparation;
further, the solid preparation is a direct-use solid preparation, a dispersible solid preparation or a soluble solid preparation;
further, the directly-used solid preparation is powder, granule, sphere, tablet or strip;
the dispersible solid preparation is wettable powder, oil dispersion powder, emulsion powder, water dispersible granule, emulsion granule or water dispersible tablet;
the soluble solid preparation is soluble powder, soluble tablets or soluble granules;
further, the liquid preparation is a solution preparation, a dispersion liquid preparation, an emulsion preparation, a suspension preparation or a multiphase preparation;
further, the solution preparation is a soluble agent, an oil agent or a film spreading oil agent;
the dispersion liquid preparation is emulsifiable concentrate, emulsion, dispersible agent or paste;
the emulsion preparation is aqueous emulsion, oil emulsion, microemulsion or fat agent;
the suspension preparation is suspending agent, microcapsule suspending agent, oil suspending agent or dispersible oil suspending agent;
the multiphase preparation is a suspension emulsion, a microcapsule suspension-suspending agent, a microcapsule suspension-water emulsion or a microcapsule suspension-suspending emulsion;
further, the seed treatment preparation comprises a seed treatment solid preparation or a seed treatment liquid preparation;
further, the seed treatment solid preparation is seed treatment dry powder or seed treatment dispersible powder;
the seed treatment liquid preparation is a seed treatment liquid, a seed treatment emulsion and/or a seed treatment suspending agent;
further, the preparation formulation is a solid preparation and/or a liquid preparation, and the solid preparation is the liquid preparation.
The invention also discloses an application of the pesticide composition and/or the preparation thereof in preventing and treating plant diseases.
Further, the plant is a cereal crop; the cereal crop is wheat, and the disease is wheat scab and wheat powdery mildew.
Further, the plant disease is a disease caused by one or more of the following pathogenic bacteria: a fungus of the phylum flagelliform, zygomycotina, ascomycotina, basidiomycotina and/or deuteromycotina;
further, the pesticidal composition and/or the formulation thereof is applied to a pathogenic bacterium to be controlled or a medium on which it grows in an effective dose.
The beneficial effects of the invention are as follows:
1) The pesticide composition has a synergistic effect on various pathogenic bacteria, can be used for preventing and treating various plant diseases, and expands the application range;
2) The pesticide composition can effectively reduce the dosage of pesticides and reduce the pesticide residue of agricultural products;
3) The pesticide composition can slow down the development of drug resistance of pathogenic bacteria and is safe to crops and other non-target organisms.
Detailed Description
The present invention will be described in more detail with reference to the following examples, but the present invention can be embodied in various forms and should not be construed as being limited to the embodiments set forth herein.
Preparation example of the formulation
Preparation example 1:20% polyoxin B. Fluoxazole cyprodinil suspension (1:1)
The weight percentages of the polyoxin B, the epoxiconazole 10%, the alkylphenol ethoxylates 3%, the fatty alcohol-polyoxyethylene ether phosphate 4%, the polycarboxylic acid sodium salt 1%, the magnesium aluminum silicate 1%, the xanthan gum 0.25%, the glycerol 5%, the sodium benzoate 0.1%, the silicone oil 0.5% and the deionized water are the rest.
The preparation method comprises the following steps: according to the formula proportion, sequentially placing active ingredients, a surfactant and other functional additives into a reaction kettle, adding water, uniformly mixing, shearing at a high speed, performing wet sanding, and finally homogenizing and filtering to obtain a suspending agent product.
Preparation example 2:24% polyoxin B-bixafen suspension (1:3)
The weight percentage of the composition is 6 percent of polyoxin B, 18 percent of bixafen, 1 percent of isomeric tridecanol polyoxyethylene ether, 2 percent of naphthalene sulfonate formaldehyde condensate, 3 percent of alkylphenol polyoxyethylene ether phosphate, 0.3 percent of carboxyethyl cellulose, 2 percent of magnesium aluminum silicate, 5 percent of glycerol, 0.25 percent of silicone oil and the balance of deionized water.
The preparation method comprises the following steps: the same as in preparation example 1.
Preparation example 3:30% polyoxin B-epoxiconazole water dispersible granule (1:2)
The composition comprises, by weight, 10% of polyoxin B, 20% of cyproconazole, 10% of sodium lignin sulfonate, 5% of naphthalene sulfonate formaldehyde condensate, 6% of white carbon black of 2% of sodium dodecyl sulfate, 25% of starch and the balance of kaolin.
The preparation method comprises the following steps: adding active ingredients into a carrier according to the formula proportion, adding a surfactant and other functional additives into the carrier, mixing, adding 10-25% of water after jet milling, and then kneading, granulating, drying and screening to obtain a water dispersible granule product; or spraying water, granulating, drying, and sieving to obtain the final product.
Preparation example 4:35% polyoxin B-bixafen water dispersible granule (1:6)
The composition comprises, by weight, 5% of polyoxin B, 30% of bixafen, 8% of naphthalene sulfonate formaldehyde condensate, 6% of sodium lignin sulfonate, 5% of nekal BX, 10% of ammonium sulfate and the balance of kaolin.
The preparation method comprises the following steps: the same as in preparation example 3.
Preparation example 5:30% polyoxin B. Furazolam wettable powder (2:1)
The composition comprises, by weight, 20% of polyoxin B, 10% of cyproconazole, 8% of sodium lignin sulfonate, 7% of alkyl naphthalene sulfonate formaldehyde condensate, 2% of sodium dodecyl sulfate, 15% of white carbon black and kaolin, and the balance of the composition.
The preparation method comprises the following steps: the active ingredients, the dispersing agent, the wetting agent and the filler are mixed according to the formula proportion, evenly stirred in a stirring kettle, and crushed and mixed evenly for many times by an airflow crusher, thus obtaining the wettable powder of the composition.
Preparation example 6:42% polyoxin B.Bispyrimidine wettable powder (1:5)
The composition comprises, by weight, 7% of polyoxin B, 35% of bixafen, 7% of naphthalene sulfonate formaldehyde condensate, 8% of sodium lignin sulfonate, 3% of nekal BX and the balance of kaolin.
The preparation method comprises the following steps: the same as in preparation example 5.
Indoor combined action test
Example 1: indoor combined action test of different medicament treatments on wheat scab
The test is based on: test reference NY/T1156.2-2006 section 2 for agricultural indoor bioassay test criteria Bactericide: test plate method for inhibiting growth of pathogenic bacterial hyphae.
Test target: fusarium graminearum (Fusarium graminearum).
Test instrument: a wet heat sterilization pot, an ultra-clean workbench, a constant temperature illumination incubator, an electric heating drum windproof drying box, a ten thousandth electronic balance, a pipetting gun, an alcohol lamp, a small beaker, a volumetric flask, a triangular flask, a culture dish (phi 9 cm), a puncher, an inoculator, a ruler and the like.
Test target culture conditions: fusarium graminearum stored at 4 ℃ in an indoor refrigerator is transferred onto a potato dextrose agar culture medium, and is activated for standby after being placed in a 26 ℃ incubator for 4 days of dark culture.
Test agent: 32% of polyoxin B original drug, 92% of bixafen original drug and 96% of cyproconazole original drug.
And (3) preparation of a medicament: the medicines are respectively dissolved and diluted by proper solvents, different proportions are designed according to the purpose of mixing and the activity of the medicines, and single medicines and each group of mixed medicines are prepared into a series of required mass concentrations.
Test replicates: 4 dishes were set for each concentration of test agent, 4 replicates each, 1 dish was repeated, and treatment without agent was set as a blank.
And (3) medicament treatment: under aseptic operation conditions, quantitatively adding the sterilized culture medium melted in advance into an aseptic conical flask according to test treatment, sequentially quantitatively sucking the liquid medicine from low concentration to high concentration, respectively adding into the conical flask, and fully shaking. Then the mixture was poured into 4 dishes in equal amounts to prepare drug-containing plates with corresponding concentrations.
Inoculating: the cultured pathogenic bacteria are sterilized by a sterilization puncher with the diameter of 5mm, bacterial cakes are cut from the edges of bacterial colonies, the bacterial cakes are inoculated in the center of a medicine-containing flat plate by an inoculator, the mycelium surface faces upwards, a dish cover is covered, and the bacterial cakes are placed in an incubator with proper temperature for culture.
Data investigation: the growth of pathogenic hyphae was investigated according to the growth of hyphae in a blank culture dish. Colony diameters were measured in centimeters (cm) using a ruler, diameters were measured once per colony using the cross-over method, averages were taken, and raw data was recorded for each treatment in duplicate.
Data statistics and analysis: according to the investigation result, the hypha growth inhibition rate of each treatment concentration on the target bacteria to be tested is calculated, wherein the unit is the calculated result of percentage (%) and two positions after decimal point are reserved.
D＝D 1 -D 2
Wherein:
d-colony growth diameter;
D 1 colony diameter;
D 2 -diameter of the bacterial cake.
Wherein:
i, hypha growth inhibition rate;
D 0 -the control colony increased in diameter;
D T -the agent-treated colonies increased in diameter.
And processing the data by adopting a probability value analysis method. Analyzing by DPS statistical analysis system to obtain virulence regression line and EC 50 And evaluating the activity of the test agent on the biological test material.
Grand cloud Pei method: evaluating the synergistic effect of the mixed medicaments according to the co-toxicity coefficient (CTC), wherein the compounded co-toxicity coefficient CTC is more than or equal to 120 and shows the synergistic effect; ctc.ltoreq.80 shows antagonism; 80< CTC <120 shows additive effect.
Co-toxicity coefficient (CTC value) of the blend was calculated:
wherein:
ati—actual measured virulence index of the mixture;
S-EC of Standard Agents 50 Milligrams per liter (mg/L);
M-EC of mixture 50 Units are milligrams per liter (mg/L).
TTI＝TI A ×P A +TI B ×P B
Wherein:
TTI-the theoretical toxicity index of the mixture;
TI A -a medicament virulence index;
P A -the percentage of agent a in the mix, in percent (%);
TI B -B agent virulence index;
P B the percentage of the B medicament in the mixture is expressed as percentage (%).
Wherein:
ctc—co-toxicity coefficient;
ati—actual measured virulence index of the mixture;
TTI-the theoretical toxicity index of the mixture.
The results of the indoor test are shown in the following table:
table 1 results of indoor combined action test of polyoxin B and bixafen against wheat scab
Test agent and ratio Regression equation (Y=) EC 50 (mg/L) Co-toxicity coefficient
Polyoxin B y＝5.2243+1.4808x 0.7056 -
Bispyrim y＝4.3903+1.2430x 3.0940 -
Polyoxin B bipyramid=1:50 y＝4.5740+1.0863x 2.4670 117.610
Polyoxin B bixafen=1:30 y＝4.6160+1.1643x 2.1372 130.518
Polyoxin B bipyramid=1:10 y＝4.7864+1.0706x 1.5832 149.441
Polyoxin B bipyramid=1:5 y＝4.9114+1.2495x 1.1774 168.003
Polyoxin B bipyramid=1:3 y＝5.0899+1.2341x 0.8445 198.443
Polyoxin B bipyramid=1:1 y＝5.1640+1.1224x 0.7143 160.876
Polyoxin B bipyramid=3:1 y＝5.2358+1.1142x 0.6142 142.353
Polyoxin B bipyramid=5:1 y＝5.2504+1.1184x 0.5972 135.597
Polyoxin B bixafen=10:1 y＝5.2240+0.9920x 0.5945 127.646
Polyoxin B bipyramid=30:1 y＝5.2226+1.1255x 0.6341 114.118
Polyoxin B bipyramid=50:1 y＝5.1992+1.0880x 0.6560 109.214
TABLE 2 polyoxin B complex pair wheat scab indoor combined action test results
The indoor test results in tables 1 and 2 show that the polyoxin and either the bixafen or the cyproconazole are compounded, and the polyoxin and the cyproconazole have obvious synergism on wheat scab in a reasonable range.
Example 2: indoor combined action test of different treatment agents on wheat powdery mildew
The test is based on: test reference NY/T1156.4-2006 section 4 for agricultural indoor bioassay test criteria Bactericide: experimental potting method for preventing and curing wheat powdery mildew.
Test target: powdery mildew (Blumeria graminis f.sp.tritici).
Test agent: selecting wheat variety Luyuan 502 pot culture with powdery mildew, numbering for standby after seedlings grow to 2-3 leaf period.
Test agent: 32% of polyoxin B original drug, 92% of bixafen original drug and 96% of cyproconazole original drug are provided by the research and development center of the sea li pharmaceutical industry group.
And (3) test preparation: the medicines are respectively dissolved and diluted by proper solvents, different proportions are designed according to the purpose of mixing and the activity of the medicines, and single medicines and each group of mixed medicines are prepared into a series of required mass concentrations.
Inoculating: and uniformly shaking out fresh spores of powdery mildew generated in 24h on the diseased wheat leaves, and inoculating the fresh spores on the treated 2-3 leaf stage potted wheat seedlings. 4 pots were treated each, 10 plants per pot.
And (3) medicament treatment: spraying the preparation onto wheat seedling, and naturally air drying. The test set-up treatment without agent served as a blank. After application, the culture is carried out under proper conditions.
Data investigation: grading investigation is carried out according to the disease condition of the blank control. The following classification method is adopted:
level 0: no disease spots;
stage 1: the area of the disease spots accounts for less than 5% of the area of the whole leaf;
3 stages: the area of the disease spots accounts for 6-15% of the area of the whole leaf;
5 stages: the area of the disease spots accounts for 16-25% of the area of the whole leaf;
7 stages: the area of the disease spots accounts for 26-50% of the area of the whole leaf;
stage 9: the area of the disease spots accounts for more than 50% of the area of the whole leaf.
Data statistics and analysis:
the disease index is calculated according to the formula (1)
Wherein:
x-disease index;
N i -leaf numbers at each stage;
i—relative grade value;
n-total leaf number was investigated.
The control effect is calculated according to the formula (2)
Wherein:
p, the prevention and treatment effect, the unit is;
CK-blank disease index;
PT-agent treatment index.
And evaluating the synergistic effect of the mixed medicament according to a grand cloud peak co-toxicity coefficient method (CTC) by referring to a biological standard method NY/1156.6-2006, wherein CTC is less than or equal to 80 and is antagonistic, CTC is 80 and CTC is 120 and is additive, CTC is more than or equal to 120 and is synergistic, and the co-toxicity coefficient (CTC) is calculated according to the formulas (3), (4) and (5).
Wherein:
ati—the measured virulence index of the mixture;
S-EC of Standard Agents 50 Milligrams per liter (mg/L);
M-EC of mixture 50 Units are milligrams per liter (mg/L).
TTI＝TI A ×P A +TI B ×P B
Wherein:
TTI-theoretical toxicity index of the mixture;
TI A -a toxicity index of the agent;
P A -the percentage of agent a in the mix, in percent (%);
TI B -toxicity index of B agent;
P B the percentage of the B medicament in the mixture is expressed as percentage (%).
Wherein:
ctc—co-toxicity coefficient;
ati—actual measured virulence index of the mixture;
TTI-the theoretical toxicity index of the mixture.
Calculating test results by adopting DPS data processing software, and respectively solving virulence regression equations, EC of single test medicament and mixed medicaments with different proportions 50 And solving co-toxicity coefficients (CTC) of the two medicaments in different proportions, and screening out the optimal proportion of the test medicaments. Record raw data for each process all repetitions.
The results of the indoor test are shown in the following table:
TABLE 3 indoor combined action test results of polyoxin B and bixafen on wheat powdery mildew
Test agent and ratio Regression equation (Y=) EC 50 (mg/L) Co-toxicity coefficient
Polyoxin B y＝4.6070+1.1420x 2.2087 -
Bispyrim y＝4.0234+1.0634x 8.2866 -
Polyoxin B bixafen=1:48 y＝4.2821+0.8620x 6.6469 118.040
Polyoxin B bixafen=1:24 y＝4.3391+0.8880x 5.5501 134.501
Polyoxin B bipyramid=1:12 y＝4.2858+0.9870x 4.2913 159.368
Polyoxin B bipyramid=1:6 y＝4.3153+1.3731x 3.1869 186.647
Polyoxin B bipyramid=1:2 y＝4.5805+1.1719x 2.2923 188.548
Polyoxin B bipyramid=1:1 y＝4.6899+1.0325x 1.9969 174.659
Polyoxin B bipyramid=2:1 y＝4.8027+0.8251x 1.7341 168.586
Multi-mildew resistantBixafen=5:1 y＝4.7224+0.9831x 1.9156 131.358
Polyoxin B bixafen=10:1 y＝4.7511+0.9393x 1.8409 128.551
Polyoxin B bipyramid=30:1 y＝4.6974+1.0182x 1.9821 114.133
Polyoxin B bipyramid=50:1 y＝4.6590+1.0851x 2.0617 108.693
TABLE 4 indoor combined action test results of polyoxin B and cyproconazole on wheat powdery mildew
Test agent and ratio Regression equation (Y=) EC 50 (mg/L) Co-toxicity coefficient
Polyoxin B y＝4.6241+1.1057x 2.1877 -
Cyproconazole y＝4.4865+1.0562x 3.0632 -
Polyoxin B, epoxiconazole=1:40 y＝4.6690+0.7712x 2.6865 112.920
Polyoxin B, epoxiconazole=1:20 y＝4.6743+0.8774x 2.3508 127.868
Polyoxin B, epoxiconazole=1:10 y＝4.7146+0.8651x 2.1376 138.270
Polyoxin B, epoxiconazole=1:5 y＝4.7453+0.9467x 1.8582 154.540
Polyoxin B, epoxiconazole=1:2 y＝4.8532+0.8148x 1.5140 178.512
Polyoxin B, epoxiconazole=1:1 y＝4.8621+1.0521x 1.3524 188.736
Polyoxin B, epoxiconazole=2:1 y＝4.8305+0.8316x 1.5990 151.224
Polyoxin B, epoxiconazole=5:1 y＝4.7515+1.1063x 1.6773 136.954
Polyoxin B, epoxiconazole=10:1 y＝4.7707+0.8908x 1.8088 124.174
Polyoxin B, epoxiconazole=20:1 y＝4.7354+0.9554x 1.8920 117.224
Polyoxin B, epoxiconazole=40:1 y＝4.6832+0.9580x 2.1412 102.889
The indoor test results in tables 3 and 4 show that the polyoxin and either bixafen or cyproconazole are compounded, so that the polyoxin and cyproconazole have obvious synergism on wheat powdery mildew in a reasonable range.
Field efficacy test
Example 3: field efficacy test of different medicament treatments on wheat scab
The test is based on: test reference NY/T1464.15-2007 section 15 of pesticide field efficacy test guidelines: the bactericide can prevent and treat wheat scab.
Test target: wheat scab (Fusarium graminearum).
Test crop: wheat (Ningmai 13).
Test site: the test is carried out on a corn poppy wheat field in Shaoxing city of Zhejiang province, the soil fertility of the test field is high, wheat is sowed in 2019 in 11 months and 12 days, and the sowing amount per mu is 18kg.
Test agent: the relevant test agents and the dosage are shown in the following table.
Cell arrangement: cell treatments for test agent, control agent and blank were randomly arranged.
Cell area and repetition: cell area 20m 2 Each treatment was repeated 4 times.
Time and frequency of application: the first application is carried out in the early stage of wheat flower lifting, the conventional spraying is carried out by adopting a industrial and agricultural-16 knapsack sprayer, the dosage per mu is 40L, and the spraying is required to be uniform during the spraying.
Experimental investigation: the control effect is investigated in the period of wheat milk maturity, 5 points are sampled on the diagonal line of each cell, 100 ears are investigated at each point, the ears are classified by the percentage of the area of the dried ears to the whole ears, and the number of the ears of each stage and the total ears are recorded.
The grading method comprises the following steps:
level 0: the whole spike is free from diseases;
stage 1: the area of the dried spike accounts for less than 1/4 of the area of the whole spike;
3 stages: the area of the dried spike accounts for 1/4 to 1/2 of the area of the whole spike;
5 stages: the area of the dried spike accounts for 1/2-3/4 of the area of the whole spike;
7 stages: the area of the dried spike accounts for more than 3/4 of the area of the whole spike.
The drug effect calculation method comprises the following steps:
safety investigation: after the pesticide is applied, the untimely observation is carried out, and each treatment agent does not see the phenomena of phytotoxicity such as yellowing, spots, deformity and the like of the wheat stem and leaf, and the wheat grows normally, so that the pesticide composition is safe to the wheat within the application dosage and application times of the test.
The results of the field efficacy test are shown in Table 5:
table 5 results of field efficacy tests on wheat scab with different agent treatments
Treatment of Dosage of active ingredient (g/hm) 2 ) Rate of sick ear Index of disease condition Control effect (%)
24% polyoxin B-bixafen suspension (1:3) 12 13.80 2.03 89.32
42% polyoxin B.Bispyrimidine wettable powder (1:5) 12 20.55 3.06 83.86
20% polyoxin B. Fluoxazole cyprodinil suspension (1:1) 8 16.45 2.54 86.64
30% polyoxin B-epoxiconazole water dispersible granule (1:2) 8 13.50 2.11 88.86
20% cyproconazole suspending agent 10 22.75 4.54 76.11
20% polyoxin B wettable powder 8 22.20 4.60 75.77
30% bixafen suspending agent 30 24.50 5.47 71.18
Blank control - 62.95 18.99 /
As can be seen from the field efficacy test in Table 5, the reasonable compounding of polyoxin B and bixafen or cyproconazole can effectively enhance the control effect on wheat scab. The wheat ear rate of the compound preparation treatment group is reduced, and the prevention and control effects are all over 83 percent.
Example 4: field efficacy test of different treatment agents on wheat powdery mildew
The test is based on: the test is carried out by referring to GB/T17980.22-2000 pesticide field efficacy test criterion (one) bactericide for controlling cereal powdery mildew.
Test object: wheat powdery mildew (Blumeria graminis f.sp.tritici).
Test crop: the wheat is mountain farming No. 22.
Test site: the wheat field in the next chapter of Taian city in Shandong province is planted with corn as the crop planted in the previous test, the soil fertility of the test land is medium, the cultivation conditions (variety, soil type and fertilization) of the test plot are uniform and consistent, and the agricultural practice of local science is met.
Test agent: the dosages of the test agent, the control agent and the active ingredients are shown in the following table.
Test cell arrangement: the cell treatment of test agent, control agent and blank control adopts random group arrangement, and each cell area is 20m 2 Each treatment was repeated 4 times.
The application method comprises the following steps: according to the experimental design, the drug is applied at the early stage of powdery mildew, the conventional spraying treatment is carried out by adopting a manual sprayer for the drug application time, and the drug application is carried out twice at 7d intervals.
The investigation method comprises the following steps: five-point sampling is fixed on the diagonal of each cell, and each point is investigated by 0.25m 2 Wheat plants, flag leaves of each plant and first leaves under the flag leaves were investigated after wheat heading, and classified according to the following classification method.
Grading standard:
level 0: no disease;
stage 1: the area of the disease spots accounts for less than 5% of the area of the whole leaf;
3 stages: the area of the disease spots accounts for 6% -15% of the area of the whole leaf;
5 stages: the area of the disease spots accounts for 16% -25% of the area of the whole leaf;
7 stages: the area of the disease spots accounts for 26% -50% of the area of the whole leaf;
stage 9: the area of the disease spots accounts for more than 50% of the area of the whole leaf;
investigation time: the disease index was investigated and recorded before the first spraying treatment, and the drug effect was investigated after 7d after the first application and 10d after the last application.
The drug effect calculation method comprises the following steps:
the efficacy is calculated according to the following formula:
the results of the field efficacy test are shown in Table 6:
TABLE 6 results of field efficacy tests of different treatments on wheat powdery mildew
The test results in Table 6 show that the preparation examples of the 35% polyoxin B.bixafen water dispersible granule (1:6) and the 20% polyoxin B.sedaxane suspending agent (1:1) have good control effect on wheat powdery mildew and are safe for wheat crops.

Claims (10)

1. The pesticide composition containing the polyoxin is characterized by comprising an active ingredient I and an active ingredient II, wherein the active ingredient I is polyoxin B, and the active ingredient II is any one of cyproconazole or bixafen.
2. The pesticide composition as set forth in claim 1, wherein the mass ratio of the active ingredient I to the active ingredient II is 1:48 to 50:1.
3. The pesticide composition as set forth in claim 1, wherein the active ingredient II is cyproconazole, and the mass ratio of the active ingredient I to the active ingredient II is 1:30 to 10:1;
preferably, the mass ratio of the active ingredient I to the active ingredient II is 1:20-10:1.
4. The pesticide composition as set forth in claim 1, wherein the active ingredient II is bixafen, and the mass ratio of the active ingredient I to the active ingredient II is 1:30-10:1;
preferably, the mass ratio of the active ingredient I to the active ingredient II is 1:24-10:1.
5. A pesticide composition according to claim 1, wherein the total weight of the pesticide composition is 1-90%, preferably 2-80% of the total weight of the pesticide composition, based on 100 wt%.
6. A pesticide composition as set forth in claim 1, wherein said pesticide composition comprises, in addition to the active ingredient, agriculturally acceptable auxiliary ingredients selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, freezing point depressants, antifoaming agents, solvents, preservatives, stabilizers, synergists, or carriers.
7. The pesticide composition of claim 1, wherein the pesticide composition can be prepared into any agriculturally acceptable formulation, and the formulation is a solid formulation or a liquid formulation;
the solid preparation comprises powder, granules, balls, tablets, strips, wettable powder, oil dispersion powder, emulsion powder, water dispersible granules, emulsion granules, water dispersible tablets, soluble powder, soluble tablets or soluble granules;
the liquid preparation comprises a soluble agent, an oil agent, a spreading oil agent, an emulsifiable concentrate, emulsion, dispersible agent, ointment, aqueous emulsion, oil emulsion, microemulsion, lipid suspending agent, microcapsule suspending agent, oil suspending agent, dispersible oil suspending agent, suspending emulsion, microcapsule suspending-suspending agent, microcapsule suspending-aqueous emulsion or microcapsule suspending-suspending emulsion;
preferably, the preparation formulation is a solid preparation and/or a liquid preparation, the solid preparation is water dispersible granules or wettable powder, and the liquid preparation is a suspending agent.
8. Use of the pesticidal composition according to any one of claims 1 to 7 and/or a formulation thereof for controlling plant diseases.
9. The use according to claim 8, wherein the plant is a cereal crop and the disease is wheat scab, wheat powdery mildew.
10. The use according to claim 8, characterized in that the pesticidal composition and/or the formulation thereof is applied in an effective dose to the pathogenic bacteria or the medium in which it is grown to be controlled.
CN202311225752.3A 2023-09-22 2023-09-22 Pesticide composition containing polyoxin and application thereof Pending CN117256615A (en)

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