CN104839180A - Fungicide composition and application thereof - Google Patents
Fungicide composition and application thereof Download PDFInfo
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- CN104839180A CN104839180A CN201510262430.5A CN201510262430A CN104839180A CN 104839180 A CN104839180 A CN 104839180A CN 201510262430 A CN201510262430 A CN 201510262430A CN 104839180 A CN104839180 A CN 104839180A
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- piperazine mycin
- shen piperazine
- captan
- component
- mancozeb
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
Abstract
The invention discloses a fungicide composition and application thereof, and relates to the technical field of pesticide compounding. Active ingredients of the fungicide composition comprise phenazine-1-carboxylic acid A and a fungicide B, wherein the fungicide B is selected from captan, mancozeb and thiram, and the weight ratio of the phenazine-1-carboxylic acid A to the fungicide B is (1:100)-(100:1). According to the fungicide composition, the compounded active ingredients A and B have remarkable synergism and can effectively reduce the application rate of each single reagent; expansion of a bacteriocidal spectrum and slowing of plant fungicide resistance are greatly facilitated, the effect preservation period can also be prolonged, and a remarkable promotional value exists. The fungicide composition can be added with other auxiliary ingredients, which are allowed to be used in pesticides, to be prepared into any agent, and can effectively prevent and control a rice sheath blight disease, rice blast, rice false smut, wheat rust, powdery mildew, wheat root rot, wheat sharp eyespot, soybean root rot, sclerotinia rot of colza, corn head smut, fusarium wilt, downy mildew, pestilence, bacterial angular leaf spot and the like.
Description
Technical field
The present invention relates to a kind of bactericidal composition and application thereof, refer to that a kind of active ingredient contains bactericidal composition and the application thereof of Shen piperazine mycin and captan, mancozeb or tmtd especially, belong to technical field of pesticide combination.
Background technology
In agricultural production, can crops healthy growth, except affecting by worm, crop smothering, also very important to the control of disease.Along with reinforcement and the sustainable development implementation of environmental protection ideas, the inexorable trend efficient, low toxicity, high activity, low-residual have become Agrochemicals.But, even if efficient germicide, be used alone for a long time, also easily make disease develop immunity to drugs, cause the problems such as dosage increasing, preventive effect reduction, lasting period shortening, be unfavorable for environment sustainable development.Therefore, how Scientific Usage of Drugs, reduces the consumption of chemical pesticide, improves drug effect, becomes the problem that plant protection art is badly in need of solving.
Shen piperazine mycin, its structural formula is as shown in (I), Shen piperazine mycin is a kind of antibiotic secreted through biological culture by pseudomonas fluorescens M18, has wide spectrum Suppressing phytopathogens and the bactericide of the dual-use function of Promoting plant growth effect simultaneously, has wide spectrum, efficiently feature.
Structural formula (I) Shen piperazine mycin
Captan (captan) also known as Cape Town, molecular formula: C
9h
8c1
3nO
2s, chemical name: N-(trichloro-methylthio)-hexamethylene-4-alkene-1,2-dicarboximide.Captan is a kind of organic sulfur series bactericidal agent, has protection and therapeutic action concurrently, does not have systemic activity.But captan single dose Long-Time Service, easily makes disease develop immunity to drugs, cause the problem that dosage strengthens, preventive effect reduces, the lasting period shortens, be unfavorable for environment sustainable development.
Mancozeb (mancozeb) also known as large life, molecular formula: C
8n
2cl
4, mancozeb belongs to thiocarbamic acid series bactericidal agent, is the wider protective fungicide of sterilization, mixedly with systemic fungicide can expand fungicidal spectrum, and delays the drug-fast generation of germ, thus reaches the infringement of protective plant from germ.
Tmtd is protective fungicide; its chemistry tetramethyl thiuram disulfide by name; tmtd belongs to dithiocarbamate bactericide; protective effect is strong; has a broad antifungal spectrum; owing to having good control efficiency to the disease of seed transmission and soil infection in seedling stage, in very long one section, be mainly used in seed treatment and soil treatment, be also widely used in foliage-spray at present.And can be composite with other protective fungicides, to various crop downy mildew, epidemic disease, anthracnose, Cereal smut, seedling stage yellow blight have good control.Although tmtd has a broad antifungal spectrum, is widely used, have higher accumulative, long-term a large amount of use separately also has very large risk.
Banded sclerotial blight is also known as moire disease, and popular name spends sufficient stalk, rotten pin pest, features spot.Be infected by Rhizoctonia solani Kuhn to fall ill, how occur under high temperature, super-humid conditions.Banded sclerotial blight causes harm seriously in South Rice Region of China, is one of Major Diseases in current Rice Production.This disease makes paddy rice not ear, or the not plump paddy of heading is more, and grain heavily declines.
Plant downy mildew is flagellum mushroom fungal disease.The melon crops such as cucumber, muskmelon, watermelon, custard squash; The Cruciferae such as Chinese cabbage, raphanobrassica, cauliflower; Spinach, lettuce and green onion class; The orchard crop such as grape, lichee has downy mildew to occur.Occur in protecting field plastic greenhouse, sporangium is propagated by air-flow rainwater throughout the year.The blade disease that downy mildew causes all directly can cause the economic loss of crop 30%-60% every year, is that in crops, one of serious disease is done in harm.
Frequent use containing single-activity component bactericide, cause most germs to create pesticide resistance, preventive effect is unsatisfactory, causes the problem that dosage strengthens, preventive effect reduces, the lasting period shortens, is unfavorable for environment sustainable development.Therefore, be badly in need of the sterilised products that a kind of novel product substitutes single-activity component, increase the resistance risk that drug effect delays disease, reduce economic loss.
Summary of the invention
It is reasonable that the technical problem to be solved in the present invention is to provide a kind of component, and synergistic effect is remarkable, and bactericidal effect is good, and drug cost is low, not easily develops immunity to drugs, to the bactericidal composition of crop safety and application thereof.
For solving the problems of the technologies described above, the invention provides technical scheme as follows:
Bactericidal composition of the present invention, its active ingredient is Shen piperazine mycin A and bactericide B, described bactericide B can be any one in captan, mancozeb or tmtd, the weight ratio of Shen piperazine mycin A and bactericide B is 1:100 ~ 100:1, being preferably weight ratio is 1:50 ~ 50:1, and more preferably weight ratio is 1:25 ~ 25:1.
Pesticidal preparations auxiliary element can also be contained, to make the formulation being applicable to agriculturally using in bactericidal composition of the present invention.Described pesticidal preparations auxiliary element is one or more in solvent, emulsifier, dispersant, stabilizing agent, antifreezing agent or wetting agent.
Bactericidal composition of the present invention can be prepared into any one formulation of applicable agricultural use by known method, reasonable formulation is dry suspending agent (DF), oil-suspending agent, aqueous emulsion, microemulsion, wetting powder (WP), water dispersible granules (WDG), suspending agent (SC), seed treatment or suspension seed-coating agent etc.
A wetting powder (WP) is can homodisperse solid powder preparation in water.Wetting powder of the present invention except adding as white carbon in former medicine, precipitated calcium carbonate, potter's clay, diatomite, outside the thinners such as attapulgite or inert substance, also have ionic or nonionic surface active agent (wetting agent, dispersant), wetting agent such as, alkylbenzenesulfonate (DBS-Na), alkylnaphthalene sulfonate (pulling open powder), alkylphenol-polyethenoxy base ether sulfate, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate, aliphatic alcohol sulfate, fatty alcohol ethylene oxide adduct sulfonate, alkyl amide taurate, aliphatic alcohol polyethenoxy base ether (JFC), content 0.1 ~ 30%, dispersant is as naphthalene sulfonate, naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), lignin and derivative sulfonate (M-9, POLYFON etc.) thereof, polyoxyethylene poly-oxygen propylene aether block copolymer, alkylphenol-polyethenoxy base ether formaldehyde condensation products sulphate (SOPA), PVA, CMC content 0.1 ~ 30%, also containing one or more materials among penetrating agent JFC, azone, thiophene ketone, penetrant t, content 0.3 ~ 30%, other auxiliary agents such as stabilizing agent, foam inhibitor, anticaking agent can also be contained.
B water dispersible granules (WDG) very fast disintegration in water, dispersion can form the granule of higher suspension dispersion.By active component, the key element such as auxiliary agent and the carrier compositions such as wetting agent, dispersant, interleaving agent, disintegrant, stabilizing agent, adhesive.Its auxiliary agent and carrier, and the compound method of precursor (preformed objects before granulation) is analogous to wetting powder and suspending agent substantially.
C suspending agent (SC) is not water soluble solid agricultural chemicals or the dispersion of immiscible liquids agricultural chemicals in water or oil.Obtained through soybean dietary fiber by active component, auxiliary agent (wetting dispersing agent, thickener, stabilizing agent, pH adjusting agent, defoamer, antifreezing agent).Wetting dispersing agent can be naphthalene or alkyl naphthalene formaldehyde condensate sulfonates (NO, NN0, MF), lignin and derivative sulfonate (M-9, POLYFON etc.) thereof, alkylphenol-polyethenoxy base ether sulfonate, polyoxyethylene polyoxypropylene base ether block copolymers, one or more in alkylphenol-polyethenoxy base phosphate, carboxymethyl cellulose (CMC), polyvinylpyrrolidone (PVP), polyethylene glycol (PEG), condensed phosphate etc., content 0.1 ~ 12%; Thickener and stabilizing agent can be one or more in xanthan gum (XG), polyvinyl alcohol (PVA), Magnesiumaluminumsilicate etc., content 0.05 ~ 0.5%; PH adjusting agent and defoamer can optionally add in right amount.
D dry suspending agent (Dry Flowable, be called for short DF), be can very fast disintegration in water, dispersion formation higher suspension dispersion granule.Preparation method: the preparation obtained by the dehydration of suspending agent.Its auxiliary agent and carrier, and the compound method of precursor (preformed objects before dehydrating) is consistent with suspending agent.
Bactericidal composition of the present invention is applicable to the pesticidal preparations preparing controlling plant diseases.Be particularly useful for making the pesticidal preparations of the water prevention rice sheath blight, rice blast, false smut, wheat rust, powdery mildew, root rotof flax, wheat sharp eyespot, rice sheath blight disease, root rot, sclerotinia rot of colza, maize head smut, capsicum wilt, cucurbits fusarium wilt, downy mildew, epidemic disease, bacterial angular leaf spot, late blight etc.
Bactericidal composition of the present invention can finished product preparation form provide, namely in bactericidal composition, each material mixes, or the composition of bactericidal composition also can provide with unit dose form, directly directly mix in bucket or tank before using, be then diluted to required concentration.
Bactericidal composition of the present invention is applied to the method for controlling disease, active principle A and B component are with above-mentioned weight ratio (1:100-100:1,1:50-50:1,1:25-25:1) use, it is used after can mixing according to the above ratio, use in proportion simultaneously or use successively in proportion.Concrete method of application can be powder injection process, spray-on process, smog method, fumigating system and seed treatment and soil treatment, ultra low volume spraying, mixes fertilizer, spreads fertilizer over the fields, fills with root.
Compared with prior art, beneficial effect of the present invention is:
1, compared with single dose, composite have obvious synergistic function, improves control efficiency;
2, composition is made up of the active ingredient that two kinds of mechanism of action are different, and bactericidal effect is good, is conducive to overcoming and delay the drug-fast generation of germ;
3, medicament mixture decreases dosage, thus reduces cost and alleviate the pollution to environment.
Instant component is reasonable, and treatment adds protective effect, and drug cost is low, and its activity and bactericidal effect are not the simple superposition of each composition activity, but has significant synergistic effect, good to crop safety, meets the security requirement of pesticidal preparations.
Embodiment
For making the technical problem to be solved in the present invention, technical scheme and advantage clearly, will be described in detail with specific embodiment below, but the present invention is limited to absolutely not these examples.The following stated is only the good embodiment of the present invention, only in order to explain the present invention, therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
The combination of active principles of different agricultural chemicals is made agricultural chemicals, is current development novel pesticide and the one of preventing and treating agriculturally resistance germ effectively and efficiently mode.After the pestsides synthesis of different cultivars, usually show a large amount of three kinds of type of action: summation action, synergistic effect and antagonism.Compound synergic is well filled a prescription, and can significantly improve actual control efficiency, and reducing the usage amount of agricultural chemicals, thus contribute to delaying the drug-fast generation speed of germ, is the important means of integrated control disease.
The present composition with Shen piperazine mycin (A) for a kind of active ingredient, bactericide (B) is another active ingredient, combine between them, to plant disease, there is obvious synergistic function, and being not only the simple superposition of two kinds of pharmacy effects, this can clearly recognize in virulence test result indoor.
In order to prevent and treat the wheat sharp eyespot in agricultural production, inventor carries out composite synergy research with Shen piperazine mycin and captan, and concrete grammar is:
Test adopts wheat sharp eyespot to be tested object.
Former medicine is mixed with the test medicine of needs, test method: fungicide bioassay pattern--Broad Bean Leaves method, with reference to " People's Republic of China's agricultural industry criteria ".Choose broad bean susceptible variety potted plant, clip same section, growing way is consistent, be with petiolate blade, put in culture dish, moisturizing is for subsequent use, experimental pharmacy is adopted former medicinal water dissolved dilution, 5 mass concentrations are set, blade is fully infiltrated 5S in pre-configured good liquid, drains unnecessary liquid, after natural air drying, cultivate by moisturizing after marks for treatment, test set not containing the process of medicament as blank.Be that 5mm bacterium cake has the one side of mycelia to be inoculated in process central vane with inoculator by diameter, 30 leaves are inoculated in each process.Protectiveness test is 24h inoculation after chemicals treatment, and curative tests is 24h inoculation before chemicals treatment.Inoculation is placed on climatic cabinate or constant temperature has in illumination humidistat in fact, cultivates under the condition of temperature 26 degrees Celsius-28 degrees Celsius, relative moisture 80%-90%.According to blank incidence, with kind of calliper record each vaccination scab length and width, with length, wide mean value represents lesion diameter record lesion diameter (unit mm). according to survey data, by preventing and treating result publicity: preventive effect=(blank diameter-process diameter)/blank diameter * 100, calculate the percentage that each process suppresses lesions grow, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC50 value of each medicament, the co-toxicity coefficient (CTC) of mixture is calculated with co-toxicity coefficient table, the activity of reagent agent to germ is evaluated with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 ﹤ CTC ﹤ 120 show as summation action.
Table 1 Shen piperazine mycin and the composite Toxicity Determination to rice sheath blight disease of captan
| Chemicals treatment | Proportioning | Virulence regression equation | EC50(mg/L) | Co-toxicity coefficient (CTC) |
| Shen piperazine mycin (A) | —— | Y＝3.4691+0.9487X | 41.0850 | —— |
| Captan (B1) | —— | Y＝3.7244+0.7676X | 45.8987 | —— |
| A:B1 | 1:100 | Y＝3.8382+0.7825X | 30.5152 | 150.2 |
| A:B1 | 1:50 | Y＝3.5285+1.0216X | 27.5660 | 166.1 |
| A:B1 | 1:25 | Y＝3.3386+1.1749X | 25.9424 | 176.1 |
| A:B1 | 1:1 | Y＝3.5291+1.0968X | 21.9323 | 197.7 |
| A:B1 | 25:1 | Y＝3.4317+1.1308X | 24.3685 | 169.3 |
| A:B1 | 50:1 | Y＝3.5791+0.9981X | 26.5152 | 155.3 |
| A:B1 | 100:1 | Y＝3.9727+0.7057X | 28.5568 | 144 |
Toxicity Determination result table 1 shows, at weight ratio 1:100-100:1, in the scope of particularly 1:50-50:1, especially Shen piperazine mycin and captan is composite has obvious synergistic effect to plant disease in the scope of weight ratio 1:25-25:1, composite synergistic effect is more obvious, and control efficiency is excellent and be better than single dose kind.
In order to prevent and treat the root rotof flax in agricultural production, inventor carries out composite synergy research with Shen piperazine mycin and mancozeb, and concrete grammar is:
Test adopts root rotof flax to be tested object.
Former medicine is mixed with the test medicine of needs, test method: by wheat seed soaked overnight, is put in 121 DEG C of moist heat sterilization 1h in 150mL conical flask.The pathogen GF1117 card punch that PDA cultivates punching is inoculated in the wheat of sterilizing, every bottle graft 6 bacterium dish, cultivates 7-10 days in 25 DEG C, ensure 12h light application time every day, and concussion every day in incubation, ensure that the amount of pathogen on wheat is consistent.The sick wheat of preparation is put in 4 DEG C after indoor are dried save backup.Pathogen adopts disease wheat inoculation method, at Bacteria culturing after five days, cultivated sick wheat is placed on one by one the base portion of wheat seedling.And at mulching soil above, kill to prevent too fast dehydration.25 DEG C of greenhouse cultivation, moisturizing of spraying water every day.
After inoculation pathogen, the incidence of 21 days statistics wheat plants, pulls up wheat seedling gently, is rinsed well by soil with water, the incidence of statistics basal part of stem.
0 grade: without disease symptom
1 grade: outer leaf sheath has obvious brown or dark brown spot, but scab is less than 1/2 diameter
3 grades: outer leaf sheath has obvious brown or dark brown spot, but scab is greater than 1/2 diameter, but internal layer leaf sheath is asymptomatic
5 grades: internal layer leaf sheath has brown or dark brown spot, but scab is less than 1/2 diameter
7 grades: internal layer leaf sheath has brown or dark brown spot, but scab is greater than 1/2 diameter, but the not dead seedling of plant
9 grades: plant is dead
Preventive effect (%)=[(contrast disease severity-process disease severity)/contrast disease severity] × 100.
Disease severity=[∑ (each sick level strain number × each disease index)/(investigating total strain number × the highest disease index)] × 100%
Disease index is calculated according to investigation result, calculate preventive effect, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC50 value of each medicament, calculate the co-toxicity coefficient (CTC) of mixture with co-toxicity coefficient table, evaluate the activity of reagent agent to germ with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 ﹤ CTC ﹤ 120 show as summation action.
Table 2 Shen piperazine mycin and the composite Toxicity Determination to root rotof flax of mancozeb
| Chemicals treatment | Proportioning | Virulence regression equation | EC50(mg/L) | Co-toxicity coefficient (CTC) |
| Shen piperazine mycin (A) | —— | Y＝3.5034+0.8755X | 51.2212 | —— |
| Mancozeb (B2) | —— | Y＝3.7361+0.7011X | 63.5148 | —— |
| A：B2 | 1:100 | Y＝3.0807+1.1707X | 43.6038 | 145.3 |
| A：B2 | 1:50 | Y＝3.4559+0.9844X | 37.0381 | 170.1 |
| A：B2 | 1:25 | Y＝3.4905+0.9868X | 33.8651 | 185.8 |
| A：B2 | 1:1 | Y＝3.4045+1.1008X | 28.1416 | 201.5 |
| A：B2 | 25:1 | Y＝3.6291+0.9324X | 29.5285 | 174.8 |
| A：B2 | 50:1 | Y＝3.2478+1.1740X | 31.0755 | 168.1 |
| A：B2 | 100:1 | Y＝3.0762+1.2151X | 38.3055 | 134.0 |
Toxicity Determination result table 2 shows, at weight ratio 1:100-100:1, in the scope of particularly 1:50-50:1, especially Shen piperazine mycin and mancozeb is composite has obvious synergistic effect to plant disease in the scope of weight ratio 1:25-25:1, control efficiency is excellent and be better than single dose kind.
In order to prevent and treat the asparagus lettuce downy mildew in agricultural production, inventor carries out composite synergy research with Shen piperazine mycin and tmtd, and concrete grammar is:
Test adopts asparagus lettuce downy mildew to be tested object.
Former medicine is mixed with the test medicine of needs, test method (plate blade method) is with reference to " People's Republic of China's agricultural industry criteria ".Choose lettuce susceptible variety potted plant, clip same section, growing way is consistent, and be with petiolate blade, put in culture dish, moisturizing is for subsequent use.Single dose and each Mixed Pharmacy are arranged 5 variable concentrations gradients (on the basis of preliminary experiment result, different with proportioning according to different agents combination, concentration arranges also different, and bacteriostasis rate sets in the scope of 5% ~ 95%).Liquid is evenly sprayed in vacuum side of blade, after liquid natural air drying, each process blade and blade is gone up dorsad, for subsequent use by being emitted on after marks for treatment in moisture preservation box, test set not containing the process of medicament as blank.Vacuum side of blade is inoculated in ready Fresh spores drop 10uL, every blade inoculation 4, often process is no less than 5 leaves, protectiveness test is inoculation in 24 hours after chemicals treatment, curative tests is in chemicals treatment inoculation in first 24 hours, then cover ware lid, being placed in temperature is that 5-7d cultivated by the constant temperature and humidity incubator of 17 ~ 22 DEG C of relative moisture more than 90%, and continuous illumination every day/night 12h alternately; According to blank incidence, record lesion diameter, calculate the percentage that each process suppresses lesions grow, by the linear regression analysis between the probit value of inhibiting rate and the logarithm value of series concentration, obtain the EC50 value of each medicament, calculate the co-toxicity coefficient (CTC) of mixture with co-toxicity coefficient table, evaluate the activity of reagent agent to germ with this.
The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergistic effect; CTC≤80 show as antagonism; 80 ﹤ CTC ﹤ 120 show as summation action.
Table 3 Shen piperazine mycin and the composite Toxicity Determination to asparagus lettuce downy mildew of tmtd
| Chemicals treatment | Proportioning | Virulence regression equation | EC50(mg/L) | Co-toxicity coefficient (CTC) |
| Shen piperazine mycin (A) | —— | Y＝3.2021+1.0776X | 46.6042 | —— |
| Tmtd (B3) | —— | Y＝2.9749+1.1454X | 58.6142 | —— |
| A：B3 | 1:100 | Y＝2.9268+1.2838X | 41.1999 | 141.9 |
| A：B3 | 1:50 | Y＝3.2979+1.0937X | 35.9923 | 162.0 |
| A：B3 | 1:25 | Y＝3.0550+1.2830X | 32.8068 | 176.9 |
| A：B3 | 1:1 | Y＝3.3936+1.1233X | 26.9196 | 192.9 |
| A：B3 | 25:1 | Y＝3.3940+1.1295X | 26.4116 | 177.9 |
| A：B3 | 50:1 | Y＝3.6133+0.9383X | 30.0530 | 155.7 |
| A：B3 | 100:1 | Y＝3.5010+0.9783X | 34.0562 | 137.1 |
Toxicity Determination result table 3 shows, at weight ratio 1:100-100:1, in the scope of particularly 1:50-50:1, especially Shen piperazine mycin and tmtd is composite has obvious synergistic effect to plant disease in the scope of weight ratio 1:25-25:1, control efficiency is excellent and be better than single dose kind.
For making the technical problem to be solved in the present invention, technical scheme and advantage clearly, within the scope of weight ratio 1:100 ~ 100:1, build the embodiment that Shen piperazine mycin is composite with captan, mancozeb or tmtd respectively, be described in detail, but the present invention is limited to absolutely not these examples.
In addition, inventor also found through experiments, Shen piperazine mycin respectively with the composite effect of captan, mancozeb or tmtd, be better than the composite effect of other antibiotics bactericide and captan, mancozeb or tmtd.In order to this problem is clearly described, within the scope of weight ratio 1:100 ~ 100:1, build other antibiotics bactericide and the composite comparative example of captan, mancozeb or tmtd, be described in detail.But because length is limited, representative several comparative examples can only be enumerated.It should be pointed out that Shen piperazine mycin respectively with the composite effect of captan, mancozeb or tmtd, be better than the composite effect of other antibiotics bactericide and captan, mancozeb or tmtd, be not only confined in limited comparative example.
Bactericidal composition of the present invention can be prepared into any one formulation of applicable agricultural use by known method, reasonable formulation is wetting powder, aqueous suspension agent, oil-suspending agent, suspended emulsion, water dispersible granules, microemulsion, aqueous emulsion, seed treatment, suspension seed-coating agent etc.In all formulas, percentage is all weight percentage.The processing technology of the various formulation of the present composition is prior art, can change to some extent according to different situations.
Below in conjunction with embodiment, comparative example, content of the present invention is described further.
One: Shen piperazine mycin and captan
Embodiment 1:20.2% Shen piperazine mycin captan suspending agent (1:100)
Component: Shen piperazine mycin 0.2%, captan 20%, polycarboxylic acids sodium 4%, fatty alcohol-polyoxyethylene ether 3%, ethylene glycol 6%, XG0.32%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 20.2% Shen piperazine mycin captan suspending agent can be obtained.
Comparative example 1:20.2% jinggangmeisu captan suspending agent (1:100)
Component: jinggangmeisu 0.2%, captan 20%, polycarboxylic acids sodium 4%, fatty alcohol-polyoxyethylene ether 3%, ethylene glycol 6%, XG0.32%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.Preparation method: the same.
Embodiment 2:16.8% Shen piperazine mycin captan water dispersible granule (1:55)
Component: Shen piperazine mycin 0.3%, captan 16.5%, polycarboxylic acids sodium 3%, lignin sodium naphthalene sulfonate 8%, anhydrous sodium sulfate 6%, kaolin supplies 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 16.8% Shen piperazine mycin captan water dispersible granule.
Embodiment 3:25.5% Shen piperazine mycin captan wetting powder (1:50)
Component: Shen piperazine mycin 0.5%, captan 25%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 8%, talcum powder complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 25.5% Shen piperazine mycin captan wetting powder.
Comparative example 2:25.5% kasugarnycin captan wetting powder (1:50)
Component: kasugarnycin 0.5%, captan 25%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 8%, talcum powder complements to 100%.Preparation method: the same.
Embodiment 4:27.9% Shen piperazine mycin captan wetting powder (1:45.5)
Component: Shen piperazine mycin 0.6%, captan 27.3%, lauryl sodium sulfate 4%, dodecyl sodium sulfate 5%, white carbon 8%, precipitated calcium carbonate complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 27.9% Shen piperazine mycin captan wetting powder.
Embodiment 5:35% Shen piperazine mycin captan dry suspending agent (1:34)
Component: Shen piperazine mycin 1%, captan 34%, polycarboxylic acids sodium 3%, sodium lignin sulfonate 12%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, kaolin complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 35% Shen piperazine mycin captan dry suspending agent.
Embodiment 6:26% Shen piperazine mycin captan water dispersible granule (1:25)
Component: Shen piperazine mycin 1%, captan 25%, sodium naphthalene sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 6%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 26% Shen piperazine mycin captan water dispersible granule.
Comparative example 3:26% kasugarnycin captan water dispersible granule (1:25)
Component: kasugarnycin 1%, captan 25%, sodium naphthalene sulfonate 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 6%, kaolin complements to 100%.Preparation method: the same.
Embodiment 7:16% Shen piperazine mycin captan suspending agent (1:15)
Component: Shen piperazine mycin 1%, captan 15%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 3%, OPEO sodium sulfonate 3%, ethylene glycol 6%, XG0.32%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 16% Shen piperazine mycin captan suspending agent can be obtained.
Embodiment 8:20% Shen piperazine mycin captan wetting powder (1:1)
Component: Shen piperazine mycin 10%, captan 10%, neopelex 4%, sodium lignin sulfonate 6%, white carbon 12%, precipitated calcium carbonate complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 20% Shen piperazine mycin captan wetting powder.
Comparative example 4:20% jinggangmeisu captan wetting powder (1:1)
Component: jinggangmeisu 10%, captan 10%, neopelex 4%, sodium lignin sulfonate 6%, white carbon 12%, precipitated calcium carbonate complements to 100%.Preparation method: the same.
Comparative example 5:20% kasugarnycin captan wetting powder (1:1)
Component: kasugarnycin 10%, captan 10%, neopelex 4%, sodium lignin sulfonate 6%, white carbon 12%, precipitated calcium carbonate complements to 100%.Preparation method: the same.
Embodiment 9:21% Shen piperazine mycin captan water dispersible granule (19:2)
Component: Shen piperazine mycin 19%, captan 2%, dodecyl sodium sulfate 4%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 6%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 21% Shen piperazine mycin captan water dispersible granule.
Embodiment 10:16% Shen piperazine mycin captan dry suspending agent (15:1)
Component: Shen piperazine mycin 15%, captan 1%, naphthalenesulfonate formaldehyde condensation compound 3%, sodium lignin sulfonate 10%, white carbon 6%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 16% Shen piperazine mycin captan dry suspending agent.
Embodiment 11:26% Shen piperazine mycin captan water dispersible granule (25:1)
Component: Shen piperazine mycin 25%, captan 1%, sodium lignin sulfonate 6%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 6%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 26% Shen piperazine mycin captan water dispersible granule.
Comparative example 6:26% jinggangmeisu captan water dispersible granule (25:1)
Component: jinggangmeisu 25%, captan 1%, sodium lignin sulfonate 6%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 6%, kaolin complements to 100%.Preparation method: the same.
Embodiment 12:18.8% Shen piperazine mycin captan suspending agent (46:1)
Component: Shen piperazine mycin 18.4%, captan 0.4%, polycarboxylic acids sodium 2%, fatty alcohol oxygen vinethene 3%, ethylene glycol 6%, XG0.32%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 18.8% Shen piperazine mycin captan suspending agent can be obtained.
Embodiment 13:20.4% Shen piperazine mycin captan suspending agent (50:1)
Component: Shen piperazine mycin 20%, captan 0.4%, dodecyl sodium sulfate 1%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 3%, ethylene glycol 6%, XG0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 20.4% Shen piperazine mycin captan suspending agent can be obtained.
Comparative example 7:20.4% kasugarnycin captan suspending agent (50:1)
Component: kasugarnycin 20%, captan 0.4%, dodecyl sodium sulfate 1%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 3%, ethylene glycol 6%, XG0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.Preparation method: the same.
Embodiment 14:24% Shen piperazine mycin captan water dispersible granule (79:1)
Component: Shen piperazine mycin 23.7%, captan 0.3%, sodium lignin sulfonate 6%, lauryl sodium sulfate 4%, anhydrous sodium sulfate 6%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 24% Shen piperazine mycin captan water dispersible granule.
Embodiment 15:20.2% Shen piperazine mycin captan wetting powder (100:1)
Component: Shen piperazine mycin 20%, captan 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 8%, kaolin complements to 100%
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 20.2% Shen piperazine mycin captan wetting powder.
Comparative example 8:20.2% polyoxin captan wetting powder (100:1)
Component: polyoxin 20%, captan 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 8%, kaolin complements to 100%.Preparation method: the same.
Two: Shen piperazine mycin and mancozeb
Embodiment 16:20.2% Shen piperazine mycin mancozeb suspending agent (1:100)
Component: Shen piperazine mycin 0.2%, mancozeb 20%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 4%, OPEO 3%, 1,2 propane diols 6%, XG0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 20.2% Shen piperazine mycin mancozeb suspending agent can be obtained.
Comparative example 9:20.2% polyoxin mancozeb suspending agent (1:100)
Component: polyoxin 0.2%, mancozeb 20%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 4%, OPEO 3%, 1,2-PD 6%, XG0.32%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.Preparation method: the same.
Embodiment 17:25.5% Shen piperazine mycin mancozeb wettable powder (1:50)
Component: Shen piperazine mycin 0.5%, mancozeb 25%, naphthalenesulfonate formaldehyde condensation compound 3%, lignin sodium naphthalene sulfonate 6%, white carbon 8%, kaolin complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 25.5% Shen piperazine mycin mancozeb wettable powder.
Comparative example 10:25.5% kasugarnycin mancozeb wettable powder (1:50)
Component: kasugarnycin 0.5%, mancozeb 25%, naphthalenesulfonate formaldehyde condensation compound 3%, lignin sodium naphthalene sulfonate 6%, white carbon 8%, kaolin complements to 100%.Preparation method: the same.
Embodiment 18:35% Shen piperazine mycin mancozeb dry suspending agent (1:34)
Component: Shen piperazine mycin 1%, mancozeb 34%, sodium lignin sulfonate 8%, lignin sodium naphthalene sulfonate 6%, white carbon 15%, Sodium Benzoate 0.2%, talcum powder supplies 100%.Additionally deionized water 150-200% is added during preparation.
Preparation method: the water adding 2.0-2.5 times of Formulation amounts, mixes each component, mixes through high speed shear, again by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate, 35% Shen piperazine mycin mancozeb dry suspending agent can be obtained.
Embodiment 19:26% Shen piperazine mycin mancozeb water dispersible granule (1:25)
Component: Shen piperazine mycin 1%, mancozeb 25%, polycarboxylic acids sodium 3%, sodium lignin sulfonate 6%, anhydrous sodium sulfate 6%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 26% Shen piperazine mycin mancozeb water dispersible granule.Comparative example 11:26% tetramycin mancozeb water dispersible granule (1:25)
Component: tetramycin 1%, mancozeb 25%, polycarboxylic acids sodium 3%, sodium lignin sulfonate 6%, anhydrous sodium sulfate 6%, talcum powder complements to 100%.Preparation method: the same.
Embodiment 20:16% Shen piperazine mycin mancozeb suspending agent (1:15)
Component: Shen piperazine mycin 1%, mancozeb 15%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 3%, fatty alcohol-polyoxyethylene ether 3%, ethylene glycol 6%, XG0.35%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 16% Shen piperazine mycin mancozeb suspending agent can be obtained.
Embodiment 21:20% Shen piperazine mycin mancozeb wettable powder (1:1)
Component: Shen piperazine mycin 10%, mancozeb 10%, dodecyl sodium sulfate 4%, lauryl sodium sulfate 4%, white carbon 20%, kaolin complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 20% Shen piperazine mycin mancozeb wettable powder.
Comparative example 12:20% tetramycin mancozeb wettable powder (1:1)
Component: tetramycin 10%, mancozeb 10%, dodecyl sodium sulfate 4%, lauryl sodium sulfate 4%, white carbon 20%, kaolin complements to 100%.Preparation method: the same.
Embodiment 22:21% Shen piperazine mycin mancozeb water dispersible granule (19:2)
Component: Shen piperazine mycin 19%, mancozeb 2%, polycarboxylic acids sodium 4%, sodium lignin sulfonate 6%, anhydrous sodium sulfate 8%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 21% Shen piperazine mycin mancozeb water dispersible granule.
Embodiment 23:26% Shen piperazine mycin mancozeb water dispersible granule (25:1)
Component: Shen piperazine mycin 25%, mancozeb 1%, lignin sodium naphthalene sulfonate 8%, neopelex 3%, anhydrous sodium sulfate 6%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 26% Shen piperazine mycin mancozeb water dispersible granule.
Comparative example 13:26% polyoxin mancozeb water dispersible granule (25:1)
Component: Shen piperazine mycin 25%, mancozeb 1%, lignin sodium naphthalene sulfonate 8%, neopelex 3%, anhydrous sodium sulfate 6%, kaolin complements to 100%.Preparation method: the same.
Embodiment 24:20.4% Shen piperazine mycin mancozeb wettable powder (50:1)
Component: Shen piperazine mycin 20%, mancozeb 0.4%, sodium naphthalene sulfonate 3%, sodium lignin sulfonate 6%, white carbon 10%, kaolin complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 20.4% Shen piperazine mycin mancozeb wettable powder.
Comparative example 14:20.4% kasugarnycin mancozeb wettable powder (50:1)
Component: Shen piperazine mycin 20%, mancozeb 0.4%, sodium naphthalene sulfonate 3%, sodium lignin sulfonate 6%, white carbon 10%, kaolin complements to 100%.Preparation method: the same.
Embodiment 25:24.3% Shen piperazine mycin mancozeb water dispersible granule (80:1)
Component: Shen piperazine mycin 24%, mancozeb 0.3%, naphthalenesulfonate formaldehyde condensation compound 3%, polycarboxylic acids sodium 3%, anhydrous sodium sulfate 6%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 24.3% Shen piperazine mycin mancozeb water dispersible granule.
Comparative example 15:24.3% tetramycin mancozeb water dispersible granule (80:1)
Component: tetramycin 24%, mancozeb 0.3%, naphthalenesulfonate formaldehyde condensation compound 3%, polycarboxylic acids sodium 3%, anhydrous sodium sulfate 6%, talcum powder complements to 100%.Preparation method: the same.
Embodiment 26:20.2% Shen piperazine mycin mancozeb wettable powder (100:1)
Component: Shen piperazine mycin 20%, mancozeb 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 8%, precipitated calcium carbonate complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 20.2% Shen piperazine mycin mancozeb wettable powder.
Comparative example 16:20.2% polyoxin mancozeb wettable powder (100:1)
Component: Shen piperazine mycin 20%, mancozeb 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 8%, precipitated calcium carbonate complements to 100%.Preparation method: the same.
Three: Shen piperazine mycin and tmtd
Embodiment 27:20.2% Shen piperazine mycin tmtd suspending agent (1:100)
Component: Shen piperazine mycin 0.2%, tmtd 20%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 3%, polycarboxylic acids sodium 3%, 1,2-PD 6%, XG 0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 20.2% Shen piperazine mycin tmtd suspending agent can be obtained.
Comparative example 17:20.2% polyoxin tmtd suspending agent (1:100)
Component: polyoxin 0.2%, tmtd 20%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 3%, polycarboxylic acids sodium 3%, 1,2-PD 6%, XG 0.3%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.Preparation method: the same.
Embodiment 28:24.3% Shen piperazine mycin tmtd water dispersible granules (1:80)
Component: Shen piperazine mycin 0.3%, tmtd 24%, naphthalenesulfonate formaldehyde condensation compound 3%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 2%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 32.4% Shen piperazine mycin tmtd water dispersible granules.
Embodiment 29:25.5% Shen piperazine mycin thiram wettable powder (1:50)
Component: Shen piperazine mycin 0.5%, tmtd 25%, dodecyl sodium sulfate 5%, lauryl sodium sulfate 5%, white carbon 6%, anhydrous sodium sulfate complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 25.5% Shen piperazine mycin thiram wettable powder.
Comparative example 18:25.5% polyoxin thiram wettable powder (1:50)
Component: polyoxin 0.5%, tmtd 25%, dodecyl sodium sulfate 5%, lauryl sodium sulfate 5%, white carbon 6%, anhydrous sodium sulfate complements to 100%.Preparation method: the same.
Embodiment 30:27.9% Shen piperazine mycin thiram wettable powder (1:45.5)
Component: Shen piperazine mycin 0.6%, tmtd 27.3%, lauryl sodium sulfate 4%, dodecyl sodium sulfate 5%, white carbon 8%, precipitated calcium carbonate complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 27.9% Shen piperazine mycin thiram wettable powder.
Embodiment 31:35% Shen piperazine mycin tmtd dry suspending agent (1:34)
Component: Shen piperazine mycin 1%, tmtd 34%, sodium lignin sulfonate 8%, lignin sodium naphthalene sulfonate 6%, white carbon 10%, Sodium Benzoate 0.2%, kaolin supplies 100%.Additionally deionized water 150-200% is added during preparation.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, mixes each component, mixes through high speed shear, again by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate, 35% Shen piperazine mycin tmtd dry suspending agent can be obtained.
Embodiment 32:26% Shen piperazine mycin tmtd water dispersible granules (1:25)
Component: Shen piperazine mycin 25%, tmtd 1%, lignin sodium naphthalene sulfonate 8%, neopelex 3%, anhydrous sodium sulfate 6%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 26% Shen piperazine mycin tmtd water dispersible granules.
Comparative example 19:26% polyoxin tmtd water dispersible granules (1:25)
Component: polyoxin 25%, tmtd 1%, lignin sodium naphthalene sulfonate 8%, neopelex 3%, anhydrous sodium sulfate 6%, talcum powder complements to 100%.Preparation method: the same.
Embodiment 33:16% Shen piperazine mycin tmtd suspending agent (1:15)
Component: Shen piperazine mycin 1%, tmtd 15%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 4%, OPEO sodium sulfonate 4%, ethylene glycol 5%, XG 0.32%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 16% Shen piperazine mycin tmtd suspending agent can be obtained.
Embodiment 34:20% Shen piperazine mycin thiram wettable powder (1:1)
Component: Shen piperazine mycin 10%, tmtd 10%, dodecyl sodium sulfate 5%, lauryl sodium sulfate 5%, white carbon 12%, precipitated calcium carbonate complements to 100%.
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 20% Shen piperazine mycin thiram wettable powder.
Comparative example 20:20% polyoxin thiram wettable powder (1:1)
Component: polyoxin 10%, tmtd 10%, dodecyl sodium sulfate 5%, lauryl sodium sulfate 5%, white carbon 12%, precipitated calcium carbonate complements to 100%.Preparation method: the same.
Embodiment 35:21% Shen piperazine mycin tmtd water dispersible granules (19:2)
Component: Shen piperazine mycin 19%, tmtd 2%, polycarboxylic acids sodium 3%, sodium lignin sulfonate 6%, anhydrous sodium sulfate 5%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 21% Shen piperazine mycin tmtd water dispersible granules.
Embodiment 36:16% Shen piperazine mycin tmtd dry suspending agent (15:1)
Component: Shen piperazine mycin 15%, tmtd 1%, neopelex 3%, sodium lignin sulfonate 12%, white carbon 8%, silicone defoaming agent 0.4%, Sodium Benzoate 0.1%, talcum powder complements to 100%.Soft water 150-200%.
Preparation method: the water adding 1.5-2.0 times of Formulation amounts, each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, dehydrate and can obtain 16% Shen piperazine mycin tmtd dry suspending agent.
Embodiment 37:26% Shen piperazine mycin tmtd water dispersible granules (25:1)
Component: Shen piperazine mycin 25%, tmtd 1%, lignin sodium naphthalene sulfonate 8%, neopelex 3%, anhydrous sodium sulfate 6%, talcum powder complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 26% Shen piperazine mycin tmtd water dispersible granules.
Comparative example 21:26% polyoxin tmtd water dispersible granules (25:1)
Component: polyoxin 25%, tmtd 1%, lignin sodium naphthalene sulfonate 8%, neopelex 3%, anhydrous sodium sulfate 6%, talcum powder complements to 100%.Preparation method: the same.
Embodiment 38:19% Shen piperazine mycin tmtd suspending agent (46:1)
Component: Shen piperazine mycin 18.4%, tmtd 0.4%, polycarboxylic acids sodium 2%, OPEO sodium sulfonate 3%, ethylene glycol 6%, XG0.32%, silicone defoaming agent 0.2%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 18.8% Shen piperazine mycin tmtd suspending agent can be obtained.
Embodiment 39:20.4% Shen piperazine mycin tmtd suspending agent (50:1)
Component: Shen piperazine mycin 20%, tmtd 0.4%, sodium naphthalene sulfonate 1%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 4%, ethylene glycol 6%, XG0.3%, silicone defoaming agent 0.3%, Sodium Benzoate 0.4%, soft water complements to 100%.
Preparation method: each component is mixed, mixes through high speed shear, then by sand mill sand milling 2-3 hour, make average grain diameter reach 1-5 micron, 20.4% Shen piperazine mycin tmtd suspending agent can be obtained.
Comparative example 22:20.4% polyoxin tmtd microemulsion (50:1)
Component: polyoxin 20%, tmtd 0.4%, sodium naphthalene sulfonate 1%, acrylic acid-acrylic acid hydroxypropyl acrylate copolymer 4%, ethylene glycol 6%, XG0.3%, silicone defoaming agent 0.3%, Sodium Benzoate 0.4%, soft water complements to 100%.Preparation method: the same.
Embodiment 40:24% Shen piperazine mycin tmtd water dispersible granules (79:1)
Component: Shen piperazine mycin 23.7%, tmtd 0.3%, sodium lignin sulfonate 6%, polycarboxylic acids sodium 4%, anhydrous sodium sulfate 6%, kaolin complements to 100%.
Preparation method: each component mixed, through mechanical crushing, then through air-flow crushing, mixes, then dries through wet granulation, can obtain 24% Shen piperazine mycin tmtd water dispersible granules.
Embodiment 41:20.2% Shen piperazine mycin thiram wettable powder (100:1)
Component: Shen piperazine mycin 20%, tmtd 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 8%, kaolin complements to 100%
Preparation method: each component mixed, first through mechanical crushing, then after air-flow crushing, mixes, and can obtain 20.2% Shen piperazine mycin thiram wettable powder.
Comparative example 23:20.2% polyoxin thiram wettable powder (100:1)
Component: polyoxin 20%, tmtd 0.2%, neopelex 3%, lignin sodium naphthalene sulfonate 6%, white carbon 8%, kaolin complements to 100%.Preparation method: the same.
Early blight of tomato, downy mildew of garpe test is prevented and treated with the pesticidal preparations that above-described embodiment, comparative example obtain.
1, test process: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This test medicine consumption establishes three concentration for the treatment of respectively according to the difference of each composition, and contrast medicament is commercially available agricultural chemicals 50% captan wetting powder, 1% Shen piperazine mycin SC and blank clean water experiment respectively.
2, test method: each plot area is 50m
2, repeat 4 times, the inspection drug effect method before dispenser after Investigation and Prevention is: with blank incidence classification investigation, every community diagonal samples, and often clicks 10 strains, records total strain number, diseased plant number and sick progression.
Adopt following stage division:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for below the 6%-25% of whole leaf area;
5 grades: lesion area accounts for below the 26%-50% of whole leaf area;
7 grades: lesion area accounts for below the 51%-75% of whole leaf area;
9 grades: lesion area accounts for less than 76% of whole leaf area;
Result of the test sees the following form 4,5:
Table 4 Shen piperazine mycin (A) and the composite control efficiency to downy mildew of garpe of captan (B)
Table 4 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and captan Complex synergism obvious, control efficiency is better than single dose kind, particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, have excellent control efficiency to downy mildew of garpe, control efficiency is better than single dose kind.
Early blight of tomato field investigation standard adopts following stage division:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for below the 6%-10% of whole leaf area;
5 grades: lesion area accounts for below the 11%-20% of whole leaf area;
7 grades: lesion area accounts for below the 21%-50% of whole leaf area;
9 grades: lesion area accounts for less than 50% of whole leaf area;
Other Statistical experiments are the same.
Table 5 embodiment compares with the control efficiency of comparative example to early blight of tomato
Inventor find other antibiotics bactericide and captan composite, within the scope of weight ratio 1:100-100:1, also there is certain Complex synergism.Because length limit, do not enumerate, only with the representational jinggangmeisu of most, kasugarnycin, polyoxin constructs comparative example and embodiment carries out preventive effect contrast, as table 5 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and captan Complex synergism are obvious, are better than other antibiotics bactericide and the composite control efficiency of captan.Within the scope of 1:50-50:1, within the scope of 1:25-25:1, piperazine mycin and captan Complex synergism are obvious, to the control efficiency of plant disease, are better than other antibiotics bactericide and the composite control efficiency of captan.
The pesticidal preparations control alternaria leaf spot of apple obtained by above-described embodiment, comparative example, target test.
1, test process: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This test medicine consumption establishes three concentration for the treatment of respectively according to the difference of each composition, and contrast medicament is commercially available agricultural chemicals 80% mancozeb wettable powder, 10% Shen piperazine mycin suspending agent and blank clean water experiment respectively.
2, test method: each plot area is 50m
2, repeat 4 times, the inspection drug effect method before dispenser after Investigation and Prevention is: with blank incidence classification investigation, every community diagonal samples, and often clicks 10 strains, records total strain number, diseased plant number and sick progression.
Adopt following stage division:
0 grade: anosis;
1 grade: lesion area accounts for less than 10% of whole leaf area;
3 grades: lesion area accounts for the 11%-25% of whole leaf area;
5 grades: lesion area accounts for the 26%-40% of whole leaf area;
7 grades: lesion area accounts for the 41%-65% of whole leaf area;
9 grades: lesion area accounts for more than 65% of whole leaf area;
Result of the test sees the following form 6,7:
Table 6 Shen piperazine mycin and the composite control efficiency to alternaria leaf spot of apple of mancozeb
Table 6 field efficacy result shows, within the scope of weight ratio 1:100-100:1, obviously, control efficiency is better than single dose kind for Shen piperazine mycin and mancozeb Complex synergism.Particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, have excellent control efficiency to alternaria leaf spot of apple, control efficiency is better than single dose kind.
Target experimental technique and investigation standard are with above-mentioned alternaria leaf spot of apple.
Table 7 embodiment compares with the control efficiency of comparative example to target
Inventor find other antibiotics bactericide and mancozeb composite, within the scope of weight ratio 1:100-100:1, also there is certain Complex synergism.Because length limit, do not enumerate, only with the representational kasugarnycin of most, polyoxin, tetramycin constructs comparative example and embodiment carries out preventive effect contrast, as table 7 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and mancozeb Complex synergism are obvious, are better than other antibiotics bactericide and the composite control efficiency of mancozeb.Within the scope of 1:50-50:1, within the scope of 1:25-25:1, Shen piperazine mycin and mancozeb Complex synergism are obvious, to the control efficiency of plant disease, are better than other antibiotics bactericide and the composite control efficiency of mancozeb.
Root rot of sugar beet, the test of balsam pear cream mildew is prevented and treated with the pesticidal preparations that above-described embodiment, comparative example obtain.
1, test process: test is carried out with reference to People's Republic of China's standard (pesticide field efficacy medicine test criterion).This test medicine consumption establishes three concentration for the treatment of respectively according to the difference of each composition, and contrast medicament is commercially available agricultural chemicals 50% thiram wettable powder, 1% Shen piperazine mycin SC and blank clean water experiment respectively.
2, test method: each plot area is 50m
2, repeat 4 times, the inspection drug effect method before dispenser after Investigation and Prevention is: with blank incidence classification investigation, every community diagonal samples, and often clicks 10 strains, records total strain number, diseased plant number and sick progression.
Root rot of sugar beet every investigation in 10 days once, adopts following stage division:
0 grade: anosis;
1 grade: block tail and root table tissue have Minimal change, pathology not yet and root root in-vivo tissue;
3 grades: root tail and root table tissue have obvious pathology, and vascular bundle is brown, but not yet lignification;
5 grades: vascular bundle is dark brown, pipe portion lignification, fertility is seriously obstructed, and old complaint has part to start to decay, but decayed portion accounts for less than 30% of block root;
7 grades: more than 30% or complete stool that block root rot part accounts for root volume are due to illness withered;
9 grades: except lobus cardiacus, most of blade is due to illness withered;
Result of the test sees the following form 8,9:
Table 8 Shen piperazine mycin (A) and the composite control efficiency to root rot of sugar beet of tmtd (B)
Table 8 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and tmtd Complex synergism obvious, control efficiency is better than single dose kind, particularly within the scope of 1:50-50:1, especially within the scope of 1:25-25:1, have excellent control efficiency to root rot of sugar beet, control efficiency is better than single dose kind.
Balsam pear cream mildew field investigation standard adopts following stage division:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of whole leaf area;
3 grades: lesion area accounts for the 5%-10% of whole leaf area;
5 grades: lesion area accounts for the 11%-25% of whole leaf area;
7 grades: lesion area accounts for the 26%-50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area;
Other statistics, experimental technique are the same.
Table 9 embodiment compares with the control efficiency of comparative example to balsam pear cream mildew
Inventor find other antibiotics bactericide and tmtd composite, within the scope of weight ratio 1:100-100:1, also there is certain Complex synergism.Because length limit, do not enumerate, only construct comparative example with the representational polyoxin of most and embodiment carries out preventive effect contrast, as table 9 field efficacy result shows, within the scope of weight ratio 1:100-100:1, Shen piperazine mycin and tmtd Complex synergism are obvious, are better than other antibiotics bactericide and the composite control efficiency of tmtd.Within the scope of 1:50-50:1, within the scope of 1:25-25:1, Shen piperazine mycin and tmtd Complex synergism are obvious, to the control efficiency of plant disease, are better than other antibiotics bactericide and the composite control efficiency of tmtd.
Inventor finds Shen piperazine mycin and captan, Shen piperazine mycin and mancozeb, Shen piperazine mycin and tmtd is composite has obvious synergistic effect to plant disease, tests its control efficiency to root rotof flax.In identical proportion 1:100-100:1, in the scope of particularly 1:50-50:1, in the scope of especially 1:25-25:1, find Shen piperazine mycin and captan, Shen piperazine mycin and mancozeb, Shen piperazine mycin and tmtd is composite also has obvious synergistic effect, to plant disease, there is obvious synergistic effect.Because length is limited, be not repeated.
The above is the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the prerequisite not departing from principle of the present invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (9)
1. a bactericidal composition, is characterized in that, containing effective constituents A and B, wherein A is Shen piperazine mycin, and B is captan, mancozeb or tmtd, and the weight ratio of described A and B is 1:100 ~ 100:1.
2. bactericidal composition according to claim 1, is characterized in that, the weight ratio of described effective constituents A and B is 1:50 ~ 50:1.
3. bactericidal composition according to claim 2, is characterized in that, the weight ratio of described effective constituents A and B is 1:25 ~ 25:1.
4. according to described bactericidal composition arbitrary in claims 1 to 3, it is characterized in that, also containing pesticidal preparations auxiliary element in described bactericidal composition, to make the formulation being applicable to agriculturally using.
5. bactericidal composition according to claim 4, is characterized in that, described pesticidal preparations auxiliary element is one or more in solvent, emulsifier, dispersant, stabilizing agent, antifreezing agent or wetting agent.
6. bactericidal composition according to claim 4, is characterized in that, the formulation of described bactericidal composition is suspending agent, dry suspending agent, oil-suspending agent, water dispersible granules, wetting powder, seed treatment or suspension seed-coating agent.
7. the application of the arbitrary described bactericidal composition of claim 1 to 6 in the pesticidal preparations of preparation controlling plant diseases.
8. the application of bactericidal composition according to claim 7, it is characterized in that, the application in the pesticidal preparations preparing the water prevention rice sheath blight, rice blast, false smut, wheat rust, powdery mildew, root rotof flax, wheat sharp eyespot, rice sheath blight disease, root rot, sclerotinia rot of colza, maize head smut, capsicum wilt, cucurbits fusarium wilt, downy mildew, epidemic disease, bacterial angular leaf spot, late blight.
9. the bactericidal composition described in claim 1 to 6 is applied to the method for controlling disease, it is characterized in that, active principle A and B uses with above-mentioned weight ratio, and it is used after mixing in proportion, use in proportion simultaneously or use successively in proportion.
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| CN105248432A (en) * | 2015-11-16 | 2016-01-20 | 周红梅 | Anti-fungal composition containing phenazine-1-carboxylic acid and dimethomorph |
| CN105580813A (en) * | 2015-12-18 | 2016-05-18 | 广东中迅农科股份有限公司 | Bactericidal composition containing fluazinam and shenqinmycin |
| CN106577680A (en) * | 2016-12-01 | 2017-04-26 | 青岛东生药业有限公司 | Bactericide and application thereof |
| CN106804158A (en) * | 2016-12-09 | 2017-06-09 | 河池市农业科学研究所 | A kind of rice blast prevention and controls |
| CN109287407A (en) * | 2018-09-14 | 2019-02-01 | 山东省农业科学院农业资源与环境研究所 | A kind of Ecological preventive way of Winter Wheat Root maize ear rot |
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| CN104886070B (en) | 2017-04-26 |
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