CN107912447A - A kind of microbicide compositions containing bacillus amyloliquefaciens, preparation method and the usage - Google Patents
A kind of microbicide compositions containing bacillus amyloliquefaciens, preparation method and the usage Download PDFInfo
- Publication number
- CN107912447A CN107912447A CN201711186051.8A CN201711186051A CN107912447A CN 107912447 A CN107912447 A CN 107912447A CN 201711186051 A CN201711186051 A CN 201711186051A CN 107912447 A CN107912447 A CN 107912447A
- Authority
- CN
- China
- Prior art keywords
- bacillus amyloliquefaciens
- compositions containing
- prochloraz
- fluoxastrobin
- microbicide compositions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/38—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< where at least one nitrogen atom is part of a heterocyclic ring; Thio analogues thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of microbicide compositions containing bacillus amyloliquefaciens, preparation method and the usage, Prochloraz and Fluoxastrobin are made into 1 × 10 respectively with methanol3The mother liquor of μ g/ml, 4 DEG C of Cord bloods are stand-by；By Bacillus amyloliquefaciens strain X60 when 30 DEG C of 180rpm shaken cultivations 36 48 of NB nutrient solutions are small, then 6000 10000rpm are centrifuged 10 minutes and are collected bacillus amyloliquefaciens X60 wet thallus, take X60 wet thallus and preservative fluid for thallus, are 1 by mass volume ratio:50 are made into microbial inoculum, and microbial inoculum finished product viable bacteria concentration is 1 × 1010‑1×1012CFU/ml；Prochloraz, Fluoxastrobin, bacillus amyloliquefaciens wet thallus are mixed, the composition being configured to containing Fluoxastrobin is 10 ~ 40 μ g/ml, Prochloraz is 5 ~ 10 μ g/ml, and bacillus amyloliquefaciens X60 is 1 × 104~1×1010CFU/ml.The present invention can handle tobacco seed and carry disease fungus, have preferable prophylaxis effect, while again environmental-friendly, can remarkably promote tobacco seedling growth.
Description
Technical field
The present invention relates to biological technical field, relates in particular to a kind of microbicide compositions containing bacillus amyloliquefaciens
Microbicide compositions containing bacillus amyloliquefaciens, also relate to the system of the microbicide compositions containing bacillus amyloliquefaciens
Preparation Method, and this contains purposes of the microbicide compositions of bacillus amyloliquefaciens in terms of tobacco seed disinfection.
Background technology
Tobacco seed is to propagate one of most important approach of tobacco pathogen.Tobacco Seedborne pathogen is typically found in seed
Surface or inside, or directly or indirectly mixed with various invalid bodies between seed.These pathogens are often tobacco diseases
First dip dyeing source.The pathogen that the tobacco seed being reported carries has rod method Pseudomonas（Alternaria sp.）, intend stem point
Mould category（Phomopsis sp.）, Acremonium（Acremonium sp.）, handle spore shell category（Zopfiella sp.）, aspergillus
（Aspergillus sp.）, Fusarium（Fusarium sp.）, Penicillium（Penicillium sp.）, gibberella category
（Gibberella sp.）, bud cladosporium category（Cladosporium sp.）, bipolaris（Bipolarissp.）Deng their meetings
Cause tobacco seed in storage phase or phenomena such as rotting, cause low percentage of seedgermination, seedling quality decline of germination period.This
Outside, the tobacco fungal disease caused by seed dispersal that is reported has anthracnose of tobacco, Alternaria alternate, tobacco downy mildew and tobacco
Black lowering etc..These diseases can cause a large amount of generations of seedling stage and field period tobacco diseases, and the production to tobacco causes sternly
Lose again.It would therefore be highly desirable to take measures to handle the pathogen that tobacco seed carries, to improve the germination quality of seed.
Microbial bacterial agent can compete nutrition, space site with pathogen, and induction plant produces disease resistance, its secondary metabolism
Product is inhibited to pathogen, has growth-promoting functions, and green, environmental protection, safety to plant, in the prevention of plant disease
In there is important application value.Therefore, the microbial bacterial agent of environmental-friendly, efficient safety is found, by itself and tobacco seed
Disease Processing agent is used in mixed way or is re-dubbed novel tobacco seed coat agent with tobacco seed Disease Processing agent, and uses it for tobacco
Nursery or tobacco seed production there is wide development space, while and the safe sustainable development of tobacco needs.
The content of the invention
One kind it is an object of the invention to overcome disadvantages mentioned above and provide can handle tobacco seed and carry disease fungus, tool
There is preferable prophylaxis effect, at the same it is again environmental-friendly, the fungicide group containing bacillus amyloliquefaciens of tobacco seedling growth can be remarkably promoted
Compound.
Another object of the present invention is to provide the preparation method of the microbicide compositions containing bacillus amyloliquefaciens.
It is still another object of the present invention to provide the microbicide compositions containing bacillus amyloliquefaciens to disappear in tobacco seed
Purposes on poison.
A kind of microbicide compositions containing bacillus amyloliquefaciens of the present invention, wherein：It is 10 ~ 40 μ g/ containing Fluoxastrobin
Ml, Prochloraz are 5 ~ 10 μ g/ml, and bacillus amyloliquefaciens X60 is 1 × 104~1×1010CFU/ml。
A kind of above-mentioned microbicide compositions containing bacillus amyloliquefaciens, wherein：It is preferred that 30 ~ 40 μ g/ml of Fluoxastrobin,
8 ~ 10 μ g/ml of Prochloraz.
A kind of preparation method of microbicide compositions containing bacillus amyloliquefaciens of the present invention, comprises the following steps：
（1）Prochloraz and Fluoxastrobin are made into 1 × 10 respectively with methanol3The mother liquor of μ g/ml, 4 DEG C of Cord bloods are stand-by；
（2）By Bacillus amyloliquefaciens strain X60 when 30 DEG C of 180rpm shaken cultivations 36-48 of NB nutrient solutions are small, then 6000-
10000rpm is centrifuged 10 minutes and is collected bacillus amyloliquefaciens X60 wet thallus, X60 wet thallus and preservative fluid for thallus is taken, by quality
Volume ratio is 1:50 are made into microbial inoculum, and microbial inoculum finished product viable bacteria concentration is 1 × 1010-1×1012CFU/ml；
（3）Prochloraz, Fluoxastrobin, bacillus amyloliquefaciens wet thallus are mixed, the composition being configured to is 10 ~ 40 containing Fluoxastrobin
μ g/ml, Prochloraz are 5 ~ 10 μ g/ml, and bacillus amyloliquefaciens X60 is 1 × 104~1×1010CFU/ml。
The preparation of the preparation method, wherein NB nutrient solutions of a kind of above-mentioned microbicide compositions containing bacillus amyloliquefaciens
Method is：Beef extract 3g, peptone 10g, add deionized water to sterilize 20 minutes through 120 DEG C to 1L, pH 6.8~7.2.
A kind of preparation method of above-mentioned microbicide compositions containing bacillus amyloliquefaciens, wherein：Preservative fluid for thallus contains
40 nonionic surface active agent of Tween of 0.01 mol/L phosphate buffers, 0.001mol/L.
A kind of preparation method of above-mentioned microbicide compositions containing bacillus amyloliquefaciens, wherein：Solve starch gemma bar
Bacterium X60, preserving number：CCTCC M 2013375, depositary institution's code：CCTCC- China typical culture collection centers, preservation list
Bit address：Wuhan, China, preservation date：On August 13rd, 2013, it is entitled：Bacillus amyloliquefaciens X60 (Bacillus amyloliquefaciensX60)。
A kind of purposes of the microbicide compositions containing bacillus amyloliquefaciens of the present invention in terms of tobacco seed disinfection.
Compared with prior art, the present invention there is obvious beneficial effect, as can be known from the above technical solutions：Solved in the present invention
Bacillus amyloliquefaciens are formulated with wet bacterium and culture presevation liquid；Prochloraz and Fluoxastrobin are made into 1 × 10 respectively with methanol3µg/
The mother liquor of ml, is diluted with the sterile water containing 0.01% polysorbate40 during use.Said composition can directly impregnated tobacco naked seed or make
On tobacco seed and matrix for tobacco seed coat agent or when being sprayed to sowing.It is true that the present invention is specific to tobacco seed cause of disease
The composition that bacterium is disinfected.Composition adds the action site to target fungi, and correlation is sterilized compared with single dose and is substantially carried
Height, can lower drug cost, reduce the pollution to environment；The growth and development of tobacco seedlings can be remarkably promoted；It can mitigate to disease fungus
Single selection pressure, potential resistance risk of the pathogen to Prochloraz, Fluoxastrobin and bacillus amyloliquefaciens can be reduced；Energy
Expand fungicidal spectrum, a variety of disease funguses carried on tobacco seed are played with the simultaneous effect controlled of a medicine.Show through live test, solve
Bacillus amyloliquefaciens strain X 60 carries disease fungus with the composition of Prochloraz and Fluoxastrobin to tobacco seed has very strong suppression
Make and use, and the prevention effect with the long period.Seed disease Disinfection Effect is than Prochloraz independent role, the independent work of Fluoxastrobin
Disinfection Effect with, bacillus amyloliquefaciens independent role and Prochloraz and Fluoxastrobin independent role is good.And Prochloraz and phonetic bacterium
Two kinds of base-materials of ester do not chemically react, and have obvious synergy or are added effect, sprouting and tobacco seedlings hair to tobacco seed
Educate safety.
Embodiment
Embodiment 1
A kind of preparation method of the microbicide compositions containing bacillus amyloliquefaciens, comprises the following steps：
（1）Prochloraz and Fluoxastrobin are made into 1 × 10 respectively with methanol3The mother liquor of μ g/ml, 4 DEG C of Cord bloods are stand-by；
（2）By bacillus amyloliquefaciens X60 (Bacillus amyloliquefaciensX60), preserving number：CCTCC M
2013375, in NB nutrient solutions（Preparation method is：Beef extract 3g, peptone 10g, add deionized water to 1L, pH 6.8~7.2,
Sterilize 20 minutes through 120 DEG C）When 30 DEG C of 180rpm shaken cultivations 36-48 are small, then 6000rpm is centrifuged 10 minutes, takes the wet bacterium of X60
Body and preservative fluid for thallus（40 non-ionic surfactants of Tween containing 0.01 mol/L phosphate buffers, 0.001mol/L
Agent）, it is 1 by mass volume ratio:50 are made into microbial inoculum, and microbial inoculum finished product viable bacteria concentration is 1 × 1010-1×1012CFU/ml；
（3）Prepare and contain 10 μ g/ml of Prochloraz, Fluoxastrobin 40 μ g/ml and bacillus amyloliquefaciens X60 in final test concentration for 1
×1010The composition of CFU/ml.
Embodiment 2
A kind of preparation method of the microbicide compositions containing bacillus amyloliquefaciens, comprises the following steps：
（1）Prochloraz and Fluoxastrobin are made into 1 × 10 respectively with methanol3The mother liquor of μ g/ml, 4 DEG C of Cord bloods are stand-by；
（2）By bacillus amyloliquefaciens X60 (Bacillus amyloliquefaciensX60), preserving number：CCTCC M
2013375, in NB nutrient solutions（Preparation method is：Beef extract 3g, peptone 10g, add deionized water to 1L, pH 6.8~7.2,
Sterilize 20 minutes through 120 DEG C）When 30 DEG C of 180rpm shaken cultivations 36-48 are small, then 6000rpm is centrifuged 10 minutes, takes the wet bacterium of X60
Body and preservative fluid for thallus（40 non-ionic surfactants of Tween containing 0.01 mol/L phosphate buffers, 0.001mol/L
Agent）, it is 1 by mass volume ratio:50 are made into microbial inoculum, and microbial inoculum finished product viable bacteria concentration is 1 × 1010-1×1012CFU/ml；
（3）Prepare and contain 8 μ g/ml of Prochloraz, Fluoxastrobin 30 μ g/ml and bacillus amyloliquefaciens X60 in final test concentration for 1
×104The composition of CFU/ml.
Embodiment 3
A kind of preparation method of the microbicide compositions containing bacillus amyloliquefaciens, comprises the following steps：
（1）Prochloraz and Fluoxastrobin are made into 1 × 10 respectively with methanol3The mother liquor of μ g/ml, 4 DEG C of Cord bloods are stand-by；
（2）By bacillus amyloliquefaciens X60 (Bacillus amyloliquefaciensX60), preserving number：CCTCC M
2013375, in NB nutrient solutions（Preparation method is：Beef extract 3g, peptone 10g, add deionized water to 1L, pH 6.8~7.2,
Sterilize 20 minutes through 120 DEG C）When 30 DEG C of 180rpm shaken cultivations 36-48 are small, then 6000rpm is centrifuged 10 minutes, takes the wet bacterium of X60
Body and preservative fluid for thallus（40 non-ionic surfactants of Tween containing 0.01 mol/L phosphate buffers, 0.001mol/L
Agent）, it is 1 by mass volume ratio:50 are made into microbial inoculum, and microbial inoculum finished product viable bacteria concentration is 1 × 1010-1×1012CFU/ml；
（3）Prepare and contain 5 μ g/ml of Prochloraz, Fluoxastrobin 10 μ g/ml and bacillus amyloliquefaciens X60 in final test concentration for 1
×106 The composition of CFU/ml.
Test example：Microbicide compositions containing bacillus amyloliquefaciens made from embodiment 1-3 disappear tobacco seed disease fungus
The experiment of poison
With reference to culture dish seedling method, the big gold dollar tobacco seed of safflower is not disinfected（Control group）, arranged by 6 × 6 square formations,
Equably it is seeded in the aseptic flat board for filling the filter paper with sterile water moisturizing（Diameter 9cm）In, it is placed in 28 DEG C of constant incubators,
12d is cultivated under daily alternate illumination, dark each 12 h, checks and record tobacco seed fungal disease Carriage.
The obtained microbicide compositions processing containing bacillus amyloliquefaciens of embodiment 1-3：Above-described embodiment 1-3 is made
Composition substitute sterile water and carry out filter paper in moisturizing aseptic flat board, be placed in 28 DEG C of constant incubators, daily alternate illumination,
12d is cultivated under dark each 12 h, checks and record tobacco seed fungal disease Carriage.
Seed disinfection result of the test shows that a kind of microbicide compositions containing bacillus amyloliquefaciens of the invention can be very
Carry out disinfection to tobacco seed well, seed disease inhibition reaches more than 96.87%.
The microbicide compositions of the present invention of table 1 are to tobacco seed disease fungus Disinfection Effect
| Processing | Incidence（%） | Disinfection Effect（%） |
| Control group | 78.5 | -- |
| Embodiment 1 | 2.46 | 96.87 |
| Embodiment 2 | 2.14 | 97.27 |
| Embodiment 3 | 3.26 | 95.85 |
The above described is only a preferred embodiment of the present invention, not make limitation in any form, Ren Hewei to the present invention
Disengaging technical solution of the present invention content, any simple modification made according to technical spirit of the invention to above example, etc.
With change and modification, in the range of still falling within technical solution of the present invention.
Claims (7)
1. a kind of microbicide compositions containing bacillus amyloliquefaciens, wherein：It is for 10 ~ 40 μ g/ml, Prochloraz containing Fluoxastrobin
5 ~ 10 μ g/ml, bacillus amyloliquefaciens X60 are 1 × 104~1×1010CFU/ml。
2. a kind of microbicide compositions containing bacillus amyloliquefaciens as claimed in claim 1, wherein：It is preferred that Fluoxastrobin 30 ~
40 μ g/ml, 8 ~ 10 μ g/ml of Prochloraz.
3. a kind of preparation method of the microbicide compositions containing bacillus amyloliquefaciens, comprises the following steps：
（1）Prochloraz and Fluoxastrobin are made into 1 × 10 respectively with methanol3The mother liquor of μ g/ml, 4 DEG C of Cord bloods are stand-by；
（2）By Bacillus amyloliquefaciens strain X60 when 30 DEG C of 180rpm shaken cultivations 36-48 of NB nutrient solutions are small, then 6000-
10000rpm is centrifuged 10 minutes and is collected bacillus amyloliquefaciens X60 wet thallus, X60 wet thallus and preservative fluid for thallus is taken, by quality
Volume ratio is 1:50 are made into microbial inoculum, and microbial inoculum finished product viable bacteria concentration is 1 × 1010-1×1012CFU/ml；
（3）Prochloraz, Fluoxastrobin, bacillus amyloliquefaciens wet thallus are mixed, the composition being configured to is 10 ~ 40 containing Fluoxastrobin
μ g/ml, Prochloraz are 5 ~ 10 μ g/ml, and bacillus amyloliquefaciens X60 is 1 × 104~1×1010CFU/ml。
4. the preparation method of the preparation method, wherein NB nutrient solutions for a kind of microbicide compositions containing bacillus amyloliquefaciens stated
For：Beef extract 3g, peptone 10g, add deionized water to sterilize 20 minutes through 120 DEG C to 1L, pH 6.8~7.2.
5. a kind of preparation method of microbicide compositions containing bacillus amyloliquefaciens as described in claim 3 or 4, wherein：
Preservative fluid for thallus is containing 0.01 mol/L phosphate buffers, 40 nonionic surface active agent of Tween of 0.001mol/L.
6. a kind of preparation method of the microbicide compositions containing bacillus amyloliquefaciens as claimed in claim 5, wherein：Xie Dian
Afnyloliquefaciens X60, preserving number：CCTCC M 2013375, it is entitled：Bacillus amyloliquefaciens X60 (Bacillus amyloliquefaciensX60)。
A kind of 7. purposes of microbicide compositions containing bacillus amyloliquefaciens in terms of tobacco seed disinfection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711186051.8A CN107912447A (en) | 2017-11-23 | 2017-11-23 | A kind of microbicide compositions containing bacillus amyloliquefaciens, preparation method and the usage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711186051.8A CN107912447A (en) | 2017-11-23 | 2017-11-23 | A kind of microbicide compositions containing bacillus amyloliquefaciens, preparation method and the usage |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107912447A true CN107912447A (en) | 2018-04-17 |
Family
ID=61896811
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711186051.8A Pending CN107912447A (en) | 2017-11-23 | 2017-11-23 | A kind of microbicide compositions containing bacillus amyloliquefaciens, preparation method and the usage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107912447A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101632373A (en) * | 2009-05-27 | 2010-01-27 | 深圳诺普信农化股份有限公司 | Azoxystrobin compound sterilization composition |
| CN101946792A (en) * | 2010-09-17 | 2011-01-19 | 龚亚慧 | Azoxystrobin-prochloraz complex bactericide and application thereof |
| CN102604864B (en) * | 2012-03-09 | 2013-06-05 | 陕西加伦多作物科学有限公司 | Microbial bactericide and application thereof |
| CN103555615A (en) * | 2013-10-18 | 2014-02-05 | 贵州省烟草科学研究院 | Bacillus amyloliquefaciens and application thereof |
| CN106135289A (en) * | 2016-06-27 | 2016-11-23 | 中国烟草总公司郑州烟草研究院 | Bacterium drug composition, preparation and application containing phenylamide, dinitroaniline series bactericidal agent and biocontrol microorganisms |
| EP3145315A1 (en) * | 2014-05-23 | 2017-03-29 | Basf Se | Mixtures comprising a bacillus strain and a pesticide |
| CN106689212A (en) * | 2016-12-14 | 2017-05-24 | 贵州省烟草科学研究院 | Bactericide composition containing azoxystrobin, and preparation method and application thereof |
| US20170196226A1 (en) * | 2014-12-29 | 2017-07-13 | Fmc Corporation | Bacillus amyloliquefaciens rti472 compositions and methods of use for benefiting plant growth and treating plant disease |
| CN107041384A (en) * | 2017-05-19 | 2017-08-15 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of bactericidal composition and its application containing bacillus amyloliquefaciens and Fluoxastrobin |
| CN107087640A (en) * | 2017-05-19 | 2017-08-25 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of bactericidal composition and its application containing bacillus amyloliquefaciens and Prochloraz |
-
2017
- 2017-11-23 CN CN201711186051.8A patent/CN107912447A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101632373A (en) * | 2009-05-27 | 2010-01-27 | 深圳诺普信农化股份有限公司 | Azoxystrobin compound sterilization composition |
| CN101946792A (en) * | 2010-09-17 | 2011-01-19 | 龚亚慧 | Azoxystrobin-prochloraz complex bactericide and application thereof |
| CN102604864B (en) * | 2012-03-09 | 2013-06-05 | 陕西加伦多作物科学有限公司 | Microbial bactericide and application thereof |
| CN103555615A (en) * | 2013-10-18 | 2014-02-05 | 贵州省烟草科学研究院 | Bacillus amyloliquefaciens and application thereof |
| EP3145315A1 (en) * | 2014-05-23 | 2017-03-29 | Basf Se | Mixtures comprising a bacillus strain and a pesticide |
| US20170196226A1 (en) * | 2014-12-29 | 2017-07-13 | Fmc Corporation | Bacillus amyloliquefaciens rti472 compositions and methods of use for benefiting plant growth and treating plant disease |
| CN106135289A (en) * | 2016-06-27 | 2016-11-23 | 中国烟草总公司郑州烟草研究院 | Bacterium drug composition, preparation and application containing phenylamide, dinitroaniline series bactericidal agent and biocontrol microorganisms |
| CN106689212A (en) * | 2016-12-14 | 2017-05-24 | 贵州省烟草科学研究院 | Bactericide composition containing azoxystrobin, and preparation method and application thereof |
| CN107041384A (en) * | 2017-05-19 | 2017-08-15 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of bactericidal composition and its application containing bacillus amyloliquefaciens and Fluoxastrobin |
| CN107087640A (en) * | 2017-05-19 | 2017-08-25 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of bactericidal composition and its application containing bacillus amyloliquefaciens and Prochloraz |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105432667B (en) | A kind of preparation method of bacillus subtilis wettable powder | |
| CN103999858B (en) | Bactericidal composition containing cyflufenamid and S-Ethyl ethylthio sulfonate | |
| CN104531574B (en) | A kind of bacillus amyloliquefaciens gfj 4 and combinations thereof | |
| CN110713951A (en) | Pantoea agglomerans strain and application thereof | |
| CN111345297B (en) | Pesticide composition for preventing and treating pineapple black rot | |
| CN106172507A (en) | Bacillus subtilis HMB19198 is in the application prevented and treated in powdery mildew of cucumber | |
| US10264796B2 (en) | Method for producing seeds of plants resistant to seedling diseases, and method for preventing the onset of and controlling seedling diseases | |
| CN113615693B (en) | Anti-anthracnose respiratory inhibitor | |
| CN114668004B (en) | Application of perillaldehyde in preparation of bactericide for preventing and treating plant diseases | |
| CN101984834B (en) | Fruit disease antiseptic preservative agent | |
| CN101836630A (en) | Pesticide composition containing amino-oligosaccharide and kresoxim-methyl and use method and application thereof | |
| EP2177600B1 (en) | Fungus having activity of controlling disease of gramineous plant, controlling agent using the same, method of controlling and biological material | |
| CN107889820B (en) | Application of 2, 6-di-tert-butyl-p-cresol as plant disease-resistant activator in apple fruits | |
| TW200536481A (en) | Microorganism controlled plant disease and plant disease controlling agent using the same | |
| CN107916237A (en) | One plant of luminous bacillus Hb1029 and its application | |
| CN107912447A (en) | A kind of microbicide compositions containing bacillus amyloliquefaciens, preparation method and the usage | |
| CN116686834A (en) | Application of quercetin in preparation of bactericide for preventing and treating plant blight | |
| Imara et al. | Performance of soil type, cyanobacterium spirulina platensis and biofertilizers on controlling damping-off, root rot and wilt diseases of moringa (Moringa oleifera lam.) in Egypt | |
| CN109619098B (en) | Rice antibacterial agent and application thereof | |
| CN101371666A (en) | Application of Pestalotiopsis guepinii Stey. toxin in herbicide | |
| Yoon et al. | The suppressive effects of calcium compounds against Botrytis cinerea in paprika | |
| CN106834194B (en) | A kind of complex micro organism fungicide for preventing and treating rice blast and preparation method thereof | |
| Deore et al. | Comparative efficacy of Trichoderma spp. for the control of powdery mildew of cluster bean | |
| JP4969961B2 (en) | Plant soil-borne disease control material using new fungi | |
| KHALIL | Efficiency of Trichoderma viride and Bacillus subtilis as biocontrol agents against root rot caused by Fusarium solani in tomato |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180417 |