TW200536481A - Microorganism controlled plant disease and plant disease controlling agent using the same - Google Patents

Abstract

The present invention relates to a microbial strain belongs to Penicillium waksmanii having an antagonistic effect on a plant pathogenic bacterium, a plant disease controlling agent containing cells of this microbial strain, a method of controlling a plant disease by using the controlling agent, and a plant disease controlling agent containing spores of a filamentous fungus having an antagonistic effect on a plant pathogenic bacterium and a mineral oil exerting no undesirable effect on the survival of the spores.

Description

200536481 (1) IX. Description of the invention [Technical field to which the invention belongs] The present invention relates to microorganisms for controlling plant diseases, plant disease control agents using the same, and methods for controlling plant diseases. [Previous technology] There are various methods to prevent plants from being attacked by pests and diseases, in order to obtain sound growth and Φ harvest of plants. The most common method is the use of chemical agents (chemical prevention methods). However, when a chemical agent is commonly used or continuously used, a plant pathogen that exhibits resistance to the chemical agent will appear, and the effect of the developed chemical agent will be lost. In addition, the commonly used chemical agents cause an excessive burden on the natural environment and also cause damage to the ecosystem. Here, in recent years, the idea of comprehensive prevention of control methods with little environmental load and the combination of physical and biological methods is no longer relied solely on chemical agents. The idea of comprehensive prevention has gradually expanded. Biological control methods, in particular, use the interactions between organisms in nature to reduce the burden on the environment. As such a biological control method, for example, a method for controlling Bacillus subtilis bacteria resistant to plant pathogens (see Japanese Patent Application Laid-Open No. 8-175920), or a Talaromyces bacterium (filamentous fungus) One method) (see Japanese Patent Application Laid-Open No. 10-229872). However, although filamentous spores, especially conidia, can be produced in large quantities, they are more susceptible to external environmental changes than chemical agents. Depending on the storage conditions of the control agent containing filamentous spores, etc., Will shorten the survival time of filamentous spores. Therefore, plant diseases containing filamentous spores -5- 200536481 (2) Control agents must be handled with a certain degree of care. Because spores are stored for a long period of time, although there are methods for low-temperature storage, reducing water content, and blocking oxygen, but the cost of storing the control agent at low temperature or blocking the oxygen around the control agent is too high, it is actually not suitable. In addition, spores are usually reduced in water content, but spores die when they are excessively dried or heated to dryness. Therefore, the use of microbial plant disease control agents requires more practical plant disease control agents that can be stored stably even at a longer period of normal temperature. φ In addition, when using microorganisms to prevent plant diseases, spread the microorganisms on plants, etc., and non-contact pathogens should not be used. At this time, although it is also possible to directly spread microorganisms, in order to easily disperse and disperse auxiliary ingredients such as clay minerals, a surfactant is added to prepare a hydrating agent, and the agent is dissolved and dispersed in water during use. However, when such a hydrating agent is dispersed, the supplementary component of the agent remains on the surface of the crop and sometimes looks like dirt. Japanese Patent Publication No. 9-506592 discloses that in order to stably maintain conidia of insect pathogenic fungi, paraffin-based light oil insect pathogenic group # products are used. However, only the entomopathogenic fungi used in Patent Document 3 are disclosed, and nothing is disclosed or indicated about the antagonism against plant pathogens. In addition, some bacteria in the genus Penicillium are known to produce antibiotic penicillin. Penicillium waksmanii is known since ancient times but does not produce penicillin. However, it is not known whether Penicillium waksmanii, which is an antagonist against plant pathogens, prevents plant diseases. [Summary of the Invention] -6-200536481 (3) Disclosure of the Invention The first invention is implemented by the above viewpoints to provide microorganisms having an antagonistic action against plant pathogenic bacteria, a plant disease control agent containing the same, and the use thereof The purpose is to prevent plant diseases. In addition, the second invention aims to provide a more practical plant disease control agent and a control method which have less environmental burden and can be stored stably even at a longer period of normal temperature. Means for Solving the Problems In order to solve the above-mentioned problems, the present inventors separated various microorganisms from a plant surface, soil, or the like, and searched for strains having an antagonistic effect on plant pathogens. As a result, the present inventors discovered that the strain belonging to Penicillium waksmanii, which is isolated from the leaf surface of the plant, has an antagonistic effect on plant pathogens, and completed the first invention by using this. In addition, 'the inventors have repeatedly reviewed the results of ® to solve the above-mentioned problems, and found that the use of filamentous fungal spores that have an antagonistic effect on plant pathogens' and mineral oils that do not cause adverse effects on the survival of the spores are used for a long period of time. The method of preserving the spores in a stable manner, isolating the spores and oxygen, can achieve the above purpose, and complete the second invention. That is, the gist of the present invention is as follows. (1) Antagonistic to plant pathogens, and belongs to the strain of Penicillium waksmanii. (2) Penicillium waksmanii FERM BP-10258 strain or mutant thereof which has an antagonistic effect on plant pathogens. 200536481 (4) (3) Plant disease control agent containing bacterial cells of the strain or mutant described in (1) or (2). (4) One or two or more of the above plant diseases are selected from the group consisting of strawberry powdery mildew, mango anthracnose, strawberry anthracnose, and tea anthracnose, and the plant disease control agent according to (3). (5) A method for controlling plant diseases by applying the plant disease control agent described in (3) or (4) on the soil or plant body of cultivated plants. φ (6) Plant disease control agents containing filamentous spores that have antagonistic effects on plant pathogens and mineral oils that do not cause adverse effects on the survival of the spores. (7) The above mineral oils are characterized by white mineral oil (6 ) Recorded plant disease control agents. (8) The above-mentioned mineral oil is a plant disease control agent according to (6) characterized by flowing paraffin refined by hydrogenation. (9) The above-mentioned mineral oil is a plant disease control agent according to (6) characterized by flowing chain # alkane purified by hydrogenation and sulfuric acid washing. (10) The filamentous fungi having an antagonistic effect on the above plant pathogenic bacteria are characterized by filamentous fungi belonging to the genus Penicillium, Talaromyces, Gliocladium or Trichoderma (6 ) Recorded plant disease control agents. (U) The filamentous fungal line Penicillium waksmanii " Talaromyces flavus' Gliocladium virens, or Trichoderma virens, which has an antagonistic effect on the above plant pathogenic bacteria, is characterized by (6) I a plant disease control agent contained therein. -8- 200536481 (5) (1 2) Penicillium waksmanii FERM BP — 10258 'Talaromyces flavus FERM P-15816, Gliocladium virens FERM P- 1 7 3 8 1, Trichoderma virens The plant disease control agent according to (6), characterized by ATCC13213, Trichoderma virens ATCC24290 or a mutant thereof. (13) The sporophyte conidia, ascospores, or thick film spores are the plant disease control agent according to (6) with φ characteristics. (14) The plant disease control agent according to any one of (6) to (13) further characterized by containing silicon dioxide. (15) A method for controlling a plant disease by applying the plant disease control agent described in any one of (6) to (14) to the soil or plant body of a cultivated plant. (16) A method for recovering filamentous fungal conidia from a cultured bacterial body with a white mineral oil. • The Penicillium waksmanii of the first invention has an antagonistic effect on plant pathogens. In addition, the Penicillium waksmanii of the first invention has such an antagonistic effect, so when applied to plants or soil, it does not cause a burden on the environment and is sustainable. To exert its effect on controlling plant diseases.

The second control agent and control method of the present invention have the advantages of less environmental load, and are 'sealed with the disease of plants cultivated in the soil, and continue to exert the advantage of a sufficient control effect. In addition, the filamentous fungal spore system in the second control agent of the present invention has the advantage that it can survive even at normal temperature for a longer period of time. _ 纟 吉 I -9-200536481 (6) The second control agent of the present invention has The advantage of stable storage at room temperature for a long period of time. Furthermore, compared with the case where a microorganism having an antagonistic effect on plant pathogens is mixed when the hydrating agent is dispersed, the second agent of the present invention has the advantage of being less contaminated with crops caused by the supplementary ingredients of the agent. Best Mode for Implementing the Invention φ The present invention will be described in detail below. 1. About the first invention of the present invention <1> The strain of the first invention of the present invention As detailed in the examples described later, the results of extensive searches of the plant surface or soil, etc., have an antagonistic effect on plant pathogens, and found that Penicillium A new strain belonging to waksmanii was named RU-1 5000 strain. # This strain was grown on a malt extract agar medium, and when cultured at 25 t for 7 days, it formed green hairy colonies with a diameter of 3 cm. The mycelium is colorless with a dark inside and no exudate. In addition, it does not grow at 37 ° C. In addition, the growth of the agar medium in Chapak yeast extract was retarded, and the surface of the formed colonies was gray, and the inner surface was brown, and the spore formation became weak. Conidiophores are erect, thin, smooth, and do not form ascus. Without branching, the stalks are about 2 to 5 branches, almost all of which are in rotation. The bottle stalks are flask-shaped short and slender heads up to 9 // m long. The conidia are 3 v m in diameter and are approximately spherical, slippery and chain-like. -10- 200536481 (7) Based on the above-mentioned mycological properties, RU-15,000 strain was identified as belonging to the Penicillium waksmanii strain. Penicillium waksmanii RU —15000 strain was deposited on November 21, 2015 under the Trusted Number FERM P — 1 025 8 at the Licensed Biological Depositary Center of the Institute of Industrial Science and Technology of Japan (East 1, Tsukuba, Ibaraki Prefecture, 1-Chome 1) Fan 1 Central No. 6), February 23, 2005 (2005), based on the Budapest Treaty, moved to an international depository and assigned a trustee number FERM BP-• 1 025 8. The first strain of the present invention has an antagonistic effect on phytopathogenic bacteria and belongs to a strain of Penicillium waksmanii. As long as it has an antagonistic effect on plant pathogenic bacteria and belongs to the strain of Penicillium waksmanii, there is no special restriction on Sll. However, it is preferable to use the strain Pe n i c i 11 i u m w a k s m a n i i RU-15000 or a mutant thereof. In the first invention, the so-called "strain having antagonism against phytopathogens" refers to a strain that has an antagonism against at least one phytopathogen and has the effect of preventing or curing a plant disease caused by the phytopathogen. Here, the term "pathogenic plant having an effect for preventing plant diseases" means that, except for the application of the strain, under the same conditions, when the plant that can infect the plant is cultivated in an environment containing pathogenic bacteria that causes the plant disease, The plant morbidity rate of the strain (refer to Formula 1 of Example 4 described later), the plant morbidity rate to which the strain is applied is low, and the so-called "disease plant having a curative effect on plant diseases" means that the same conditions are applied except the application of the strain When cultivating plants infected with the pathogenic bacteria that caused the plant disease, the incidence of plants with the strain -11-200536481 (8) was lower than that of plants without the strain. The so-called "pathogenic strains having the effect of preventing or curing plant diseases caused by phytopathogenic bacteria" specifically include, for example, the control rate when the same experiment as described in Example 4 is performed later, usually 20 or more, preferably 40 or more, 60 or more Above preferred strains.

The so-called "phytopathogenic bacteria" in the first invention is not limited as long as Penicillium waksmanii exhibits an antagonistic effect. Examples include Sphaerotheca aphanis var_ aphanis, which causes strawberry powdery mildew, Glomerella cingulata, which causes strawberry anthracnose, or C ο 11 etotrichumacutatum, C ο 11 etotrichum theae-sinensis that causes tea anthracnose, C ο 11 e to tr i chum gleosporioides that causes anthracnose of mango o In addition, the so-called "plant disease" is only as long as it shows an antagonistic effect by Penicillium waksmanii The plant diseases caused by phytopathogens are sufficient, and there are no particular restrictions. Examples include powdery mildew of strawberry (Sphaerotheca aphanis var. Aphanis), powdery mildew (Sphaerotheca cucurbitae), powdery mildew (Sphaerotheca rosae), etc. , Strawberry anthracnose (Glomerella cingulata), melons anthracnose (Colletotrichum orbiculare), tea anthracnose (Colletotrichum theae-sinensis), mango charcoal scar disease (

Colletotrichum gleosporioides), Glomerella cingulata, Colletotrichum gleosporioides or Colletotrichum acutatum, etc. or grapes caused by the same, same or similar pathogenic bacteria of these plant pathogens 200536481 (9) powdery mildew (Uncinula necator), powdery mildew (Erysiphe cichoracearum), green pepper powdery mildew (Oidiopsis sicula), grape late rot (Glomerella cingulata), tomato black spot root rot (Colletotrichum coccodes), etc. A "mutant" in the present invention is only a strain having the mycological properties of the Penicillium waksmanii FERM BP-1 025 8 (RU-15,000) strain as described above and having an antagonistic effect on plant pathogenic bacteria, It also includes all mutants derived from the Penicillium waksmanii FERM BP-10258 strain. Mutations include natural mutations or artificial mutations such as chemical mutants or ultraviolet rays. In addition, the term "strain" in the description below may sometimes be used as meaning "Strain or mutant". <2> Chapter A control agent of the present invention The first control agent of the present invention has an antagonistic effect on phytopathogenic bacteria, and contains a plant disease control agent belonging to a strain belonging to the Penicillium waksmanii strain. The first control agent of the present invention is only required to have a phytopathogenic effect Antagonistic effect, and those who contain the bacterial cells belonging to the Penicillium waksmanii strain are not particularly limited, and can be those containing only one such strain or those containing multiple such strains. As used in The strain of the first control agent of the present invention may be, for example, a strain of Penicillium waksmanii FERM BP — 1 02 5 8 or a mutant thereof, and a strain of Penicillium waksmanii FERM BP — 10258 is particularly preferred. -13- 200536481 (10) Article A strain used in the present invention is cultured by the same method as the conventional method for cultivating microorganisms. The culture method may be a method for proliferating bacterial cells. 'Either method can be used regardless of the type of culture medium or culture conditions. For solid culture, Potato glucose agar medium, capecco agar medium, and malt agar medium can be used. lt; t standing culture, in liquid culture 'can use potato dextrose liquid medium, Chapek liquid medium, malt liquid medium, etc., shake culture at 2 5 r, in addition, when a large number of culture Φ' can use bran, Wheat, barley, soy flour, etc., were cultured at 25 ° C. When the strain used in the first invention is a yeast extract and a malt extract liquid culture medium and cultured at 25 ° C for 7 days, the spores concentration is converted, for example, a culture solution of lxlO6 to lxl08cfu / ml can be obtained. Although the culture obtained by culturing can be used as it is, it is preferable to use the culture by separating bacterial cells such as centrifugation. That is, in addition to the bacterial cells themselves of the fungus system used in the first control agent of the present invention, the suspension, culture solution, or any of these types of concentrates, pastes, dry matter, and dilutions are all can. However, from the standpoint of the shelf life of the control agent product, a dry powder prepared by naturally drying, spray-drying, freeze-drying, etc. the fungus body is suitable, especially the moisture content is 10% by weight or less, and 5% by weight or less. Dry powder is particularly preferred. The first live strain of the strain or its mutant system used in the control agent of the present invention. In addition, although the viable bacteria of the strain used in the control agent of the first aspect of the present invention may not be spores, spores are preferable from the viewpoint of the preservation of the control agent product. Therefore, in order to form spores, the culture conditions such as the composition of the culture medium at the end of the culture, the pH of the culture medium, the culture temperature, the humidity of the culture, and the oxygen concentration at the time of culture -14- 200536481 (11) are adjusted to the conditions suitable for the formation of the spores. Better. When the spores of the first invention are used, from the standpoint of the preservation property of the control agent, the spores water content is preferably 10% by weight or less, and preferably 5% by weight or less. The concentration of penicillium contained in the control agent of the first aspect of the present invention is not particularly limited as long as the effect of the first aspect of the present invention is not impaired, but when diluted to a factor of 500 to 2000, it is converted into a spore concentration, and 1 X It is preferably from 103 to 1 x 101 Gcfu / ml, and particularly preferably from lxlO4 to lxl08 cfu / ml. φ The composition of the control agent of the first aspect of the present invention may contain any substance such as a carrier, a surfactant, a dispersant, and a supplementary agent, in addition to the bacterial cells and spores of the first aspect of the present invention, as long as it does not interfere with the effects of the first aspect of the present invention. . Examples of the carrier include solid carriers such as clay, talc, bentonite, diatomaceous earth, carbon white, clay, pumice, slaked lime, silica sand, ammonium sulfate, urea, sodium bicarbonate, and sodium sulfate. Examples of the above-mentioned surfactants and dispersants include alkylphenyl ethers of addition polyethylene oxide, alkyl ethers of addition polyethylene oxide, and higher fatty acids of addition polycycloethylene oxide. Esters, sorbitan higher fatty acid esters of addition polyethylene oxide, non-ionic surfactants such as tristyrene phenyl ether of addition polyethylene oxide, addition polyethylene oxide Sulfuric acid ester salt of alkyl phenyl ether, metal salt of alkylbenzene sulfonic acid, sulfate salt of higher alcohol, sodium alkylnaphthalenesulfonate, sodium lignosulfonate, formaldehyde condensate of sodium alkylnaphthalenesulfonate, isobutylene -An ionic surfactant or dispersant such as a copolymer of maleic anhydride. Examples of the supplement include carboxymethyl cellulose, polyethylene glycol, gum arabic, starch, and lactose. The dosage form of the first control agent of the present invention is not particularly limited, and may be, for example, a form of -15-200536481 (12) powder, hydration agent, emulsion, suspension, granule and the like which are usually adopted by pesticides. The method for manufacturing the first control agent emulsion of the present invention is not particularly limited, and it can be produced by mixing the dried Penicillium spores in an organic solvent containing a surfactant and preparing a suspension. Related surfactants. There is no particular limitation as long as it does not inhibit spore germination and growth. For example, polyethylene oxide sorbose φ alkyd monolaurate, polyethylene oxide monolaurate, Any one of polyethylene oxide ethylene glycol monooleate or a mixture of two or more. In addition, as the relevant organic solvent, for example, vegetable oils such as soybean oil, rapeseed oil, castor oil, cottonseed oil, palm oil, and safflower oil, spindle oil, barite oil, light white oil, mineral spirits, and mineral turpentine can be used. Mineral oil such as naphthenic oil, paraffin oil, machinery oil for pesticides, polymers such as ethylene or isobutylene, silicone oil, etc., or two or more of them. The method for controlling plant diseases according to the first aspect of the present invention is only a method of applying the control agent of the first aspect of the present invention on cultivated plants # soil or plant body, and is not particularly limited. For example, the control agent of the first aspect of the present invention may be used The first control agent of the present invention can be applied by mixing, dispersing or injecting soil equivalent to cultivated plants, or by applying or dispersing the first control agent of the present invention directly to a plant body to apply the first control agent of the present invention. Agent. Here, when applied to the soil, the first control agent of the present invention may be used to grow plants after planting the soil, and after planting plants to the soil, they may be applied to the soil. When dispersing the control agent of the first invention, the control agent of the first invention can be diluted with an appropriate amount of water and used before use. -16- 200536481 (13) In addition, the application amount of the control agent and pesticide composition of the first invention is only required to exert the effect of the first invention, and there is no particular limitation. When it is dispersed and treated on the soil, Soil, converted to the concentration of penicillium spores, usually a control solution of lx103 to lxl01GcfU / ml, 50 to 700L, preferably 100 to 300L. 2. About the second invention 41 <1> Filamentous spores having an antagonistic effect on phytopathogenic bacteria The plant disease control agent of the second invention contains filamentous spores having an antagonistic effect on phytopathogenic bacteria. In the second invention, the "filamentous fungi having an antagonistic effect on plant pathogens" means filamentous fungi that have an antagonistic effect on at least one of the pathogenic bacteria of plant diseases. The filamentous fungi of the second aspect of the present invention prevent or cure plant diseases caused by the pathogenic bacteria by exerting an antagonistic effect on the pathogenic bacteria. The term "prevention of plant diseases" herein refers to the application of the filaments to plants that have not been infected with pathogenic bacteria or exhibited disease symptoms when they are cultivated under the same suitable conditions except for the filamentous fungi. The disease level of the fungus plant is low. In addition, the above-mentioned "cure plant disease" refers to a plant that develops disease symptoms after being infected with a pathogenic fungus. When the filamentous fungus is applied, it is cultivated under the same suitable conditions than a plant to which the filamentous fungus is applied. The disease level of the plant is low. As the filamentous fungus used in the second invention, there is no particular limitation as long as the filamentous fungus has an antagonistic effect on plant pathogenic bacteria, and examples thereof include Penicillium, Talaromyces, and Gliocladium (-17 -200536481 (14)

Filamentous bacteria of the genus Gliocladium or Trichoderma, which have antagonistic effects on plant pathogens. As the filamentous lineage belonging to the genus Penicillium, a Penicillium waksmanii RU-15000 (FERM BP-1 025 8) strain or a mutant thereof is suitable. As the filamentous lineage belonging to the genus Talaromyces, TalaromycesflavusY-9401 (FERMP-1 5 8 1 6) or a mutant thereof is suitable. As a filamentous strain belonging to the genus Gliocladium, Gliocladium virens G2 (FERM P-17381) or a mutant thereof is used as a filamentous strain belonging to the genus Trichoderma The strain is preferably Tricho derma virens ATCC132 13 ^ Tricho derma virens ATCC24290 or a mutant thereof. The "mutant of a certain strain" in the second invention, as long as it has the same mycological properties as the strain and has an antagonistic effect on plant pathogenic bacteria, it can also include all the strains derived from the strain. mutant. Mutations include natural mutations or artificial mutations by chemical mutagens or UV rays.

Penicillium waksmanii FERM BP — 1 02 5 8 was deposited by the applicant on November 21, 2015 with a trustee number of FERM P — 1 025 8 at the Licensed Biological Depositary Center (Institute of Industrial Science and Technology General Research, Ibaraki, Japan) East 1 — 1 — 1 Central 6th, Tsukuba Prefecture) February 23, 2007 (2005). Based on the Budapest Treaty, it was transferred to an international depository and assigned a trust number FERM BP — 1 02 5 8. In addition,

Talaromyces flavus Y-9401 was deposited by the applicant on September 2, 2008 under the Trusted Number FERM P — 15816 at the Licensed Microbial Depository Center of the Institute of Biotechnology, Industrial Technology Institute, Ministry of International Trade and Industry (now -18- 200536481 (15) Institute of Industrial Science and Technology, Institute of Industrial Science and Technology, Japan In addition, G 1 i 〇c 1 adiumvirens G 2 was deposited by the applicant on April 30, 2011 under the Trusted Number FERM P-17381. (Industrial Corporation for Industrial Science and Technology, General Manager J Biotrust Center).

Trichoderma virens ATCC13213 and Trichoderms ATCC24290 are available from the American type collection (ATCC, Virginia Asia, United States of America 201] Manassas 10801 University Boulevard, respectively. The second plant disease control agent of the present invention may be one containing only one filamentous fungus having an antagonistic effect on pathogenic bacteria, or one containing two such strains. In addition, the filamentous fungi used in the second invention can be used as examples of commercially available fungicides, and the spore-based filamentous fungi spores used in the second invention can also be used. Ascomycetes, ascospores, thick film spores. The plant disease of the second invention may contain only one spore species, or two or more species. The spore system of the second invention is obtained from the above-mentioned filamentous fungus culture. The culture of the filamentous fungi of the second invention can be carried out by the same method as the usual method of filamentous fungi. The culture method is only required to increase the number of cells. Regardless of the type of culture medium or culture conditions, any method can be used for solid culture, such as potato glucose agar medium, agar medium, and malt agar medium. Zhongyu Heisei entrusted to trade: franchise virens culture 0-2209 It can be cultivated on plant species such as D conidium control agent. When Peike was raised, -19- 200536481 (16) Potato glucose can be used Liquid culture medium, Chapek liquid culture medium, malt liquid culture medium, and the like are shaken. In addition, when mass culture is carried out, static culture such as art skin, wheat, barley, and soybean flour can be used. However, in order to spore the filamentous fungus, it is preferable to adjust the culture conditions such as the composition of the culture medium, the pH of the culture medium, the culture temperature, the culture humidity, and the oxygen concentration during the cultivation to suit the spore formation conditions. The culture obtained by culturing is to use a sieving machine or φ centrifugal separation of the liquid culture to separate and recover the spores. The method for recovering spores from a solid culture is not particularly limited. For example, a spore-containing solid culture is treated with a spreader having a mesh size of 0.25 mm, and the culture carrier is removed to obtain a spore concentrated solution. Alternatively, a solid culture is added to the flowing paraffin in the container, and after stirring sufficiently, the solution is tangled with a cotton cloth to obtain a spore suspension. In addition, the cotton cloth residue was re-added into the flowing paraffin, and after being fully stirred, the solution was reeled out with a cotton cloth. By repeating this operation, the spore recovery rate recovered from the filamentous fungus culture could be improved. Φ In addition, according to another aspect of the second aspect of the present invention, the conidia of the filamentous fungus are recovered from the cultured bacterial body material using white mineral oil. This method is described below. The white mineral oil in the container containing the solid culture containing filamentous conidia was added, and after stirring well, the solution was twisted out with a cotton cloth to obtain a spore suspension. In addition, the white cotton residue was added to the white mineral oil again, and after the mixture was thoroughly stirred, the solution was reeled out with a cotton cloth. By repeating this operation, the recovery rate of conidia recovered from the filamentous fungus culture could be improved. <2> Mineral oil that does not cause adverse effects on the survival of filamentous spores-20-200536481 (17) The second plant disease control agent of the present invention contains the aforementioned filamentous spores and does not cause adverse effects on the survival of the spores mineral oil. The mineral oil used in the second invention is not particularly limited as long as it does not cause any adverse effects on the survival of the filamentous fungal spores. As the mineral oil used in the first invention, for example, a colorless and transparent white mineral oil 'is preferably a paraffin refined by hydrogenation, or a purified paraffin purified by hydrogenation and resulfurization. Any of the above-mentioned mineral oils are commercially available and are generally easily available. The first one is a white mineral oil of mobile key compound used in the present invention, which has a kinematic viscosity at 40C in the range of 3 to 20mm2 / s, and a boiling point in the range of 240 to 400C. Depending on the purification method, various mobile paraffins can be obtained with a few differences in properties. For example, through the steps of solvent washing, high-pressure hydrogenation, and sulfuric acid washing, the aromatic components in the components can be reduced, and high paraffin paraffins can be obtained. For many years, in addition to agricultural uses, mobile paraffins have also been designated as food additives' as well-approved substances. It is known that mobile paraffins have different effects on plants or animals depending on their viscosity. The lower the dynamic viscosity, the smaller the effect on the soft leaves of herbs. In addition, the higher the viscosity, the higher the effect on insects or spider mites. However, different types of mobile paraffins have little effect on filamentous fungal spores. In the plant disease control agent of the second invention, various mobile paraffins can be used. <3> Plant disease control agent The second plant disease control agent of the present invention contains a plant containing a filamentous spore to a plant pathogen -21-200536481 (18), and a mineral oil which does not cause adverse effects on the survival of the spore Disease control agents. The spore concentration of the filamentous fungi contained in the plant disease control agent of the second aspect of the present invention is not particularly limited as long as the effect of the second aspect of the present invention is not impaired. It is converted into a spore concentration of 1 X 1 04 to 1 X 1 01 Gcfu / ml is preferred, and 1 X 106 to 1 X 1 09 cfU / ml is particularly preferred. Because in this range, the effects of the second invention will be more fully exhibited. φ In addition, the plant disease control agent of the second invention is only required to exhibit the effects of the second invention. The content of the mineral oil is not particularly limited, but it is 80 to 99% by weight based on the total amount of the control agent. Preferably, it is 85 to 95% by weight. Because in this range, a stable preparation can be obtained. Regarding use, it can be diluted with water to 500-4000 times, and can be spread on plants. In addition, the plant disease control agent of the second aspect of the present invention may contain any ingredients other than the filamentous fungus spores and the mineral oil as long as the effects of the second aspect of the present invention are not hindered. Examples of such optional ingredients include additives such as silicon dioxide powder, fine clay minerals, and anionic, cationic, and amphoteric surfactants. When these additives and surfactants are contained, the filamentous fungal spores are uniformly dispersed in the mineral oil, and the stability of the plant disease control agent product of the second invention can be improved. In addition, the powder of monoxide (for example, "Carplex" (manufactured by Degussa)) suppresses the precipitation of spores in the preparation during storage, and it is easy to handle the preparation from a bottle during use. There is no particular limitation on the method of manufacturing the second control insecticide emulsion of the present invention. For example, it can be mixed in the mineral oil of the second invention of the present invention with a surfactant-22-200536481 (19) and mixed with the collected Penicillium spores. , Prepared by preparing a suspension. The plant disease to which the plant disease control agent of the second invention thus obtained is applicable is a plant disease caused by the pathogenic bacteria infected by the filamentous fungi of the present invention to infect the plant, and there is no particular limitation on the filamentous fungi of the second invention Among pathogenic bacteria exhibiting an antagonistic effect, plant diseases caused by infection of plants with pathogenic bacteria belonging to fungi are particularly preferable. As a plant disease to which the plant disease control agent of the second invention is applied, for example, a filamentous fungus belonging to the genus Penicillium, Talaromyces, Gliocladium or Trichoderma can be shown Plant diseases caused by infecting plants with active pathogenic bacteria are preferably plant diseases caused by infection of plants with pathogenic bacteria that show antagonistic effects, such as Penicillium waksmanii, Talaromyces f 1 avus, Gliocladium virens, or T richoderma virens.

Penici 11 i um waksm ani i FER Μ BP — 1 02 5 8. Plant diseases caused by infected plants are particularly good. Examples of plant diseases caused by pathogenic bacteria that show resistance to Penicillium waksmanii include plants such as strawberry powdery mildew, powdery mildew, powdery mildew and other powdery mildew, strawberry anthracnose, melons anthracnose, and tea anthracnose , Anthracnose of mango, anthracnose of persimmon, anthracnose of stone fruit, and tomato leaf mold. Talaromyces flavus is a pathogenic bacterial infection exhibiting detergency action. -23- 200536481 (20) Plant diseases caused by plants include, for example, strawberry powdery mildew, tomato leaf mold, and tea anthracnose. In addition, examples of plant diseases caused by infection of plants with pathogenic bacteria exhibiting antagonistic effects by Gliocladium virens or Trichoderma virens include, for example, leaf rot of kale, which belongs to the rice family, leaf rot and sclerotinia sclerotiorum, which belongs to the field of rape Rhizobium of broccoli, Rhizobium of cabbage, Rhizoctonia sclerotiorum and Sclerotinia sclerotiorum of cabbage, Verticillium wilt of white radish, White silkworm disease and Fusarium wilt of Onion belonging to the family of 100φ, and gray of onion Rot disease, rot of plant spinach belonging to the family Chenopodiaceae, blight and wilt disease, brown rot disease of yam belonging to the family Dioscoreaceae, wilting disease of carnations belonging to the family Dianthus, wilting disease of parsley belonging to the family Asteraceae, Root rot of lettuce belonging to Asteraceae, wilt of tomato belonging to Solanaceae, root rot wilt, brown root rot and half body wilt, half body wilt of eggplant, blight of potato, watermelon of melon family Wilt disease, cantaloupe disease and black-spot root rot, purple feather disease of sweet potatoes belonging to the Convolvulaceae family, root rot of the genus Astragalus family, and chlorosis of strawberries belonging to the Rosaceae family The feathers pear scald disease. <4> Application method of plant disease control agent of the second invention and method for preventing disease by applying plant disease control agent of second invention The plant disease control agent of the second invention controls various plants as described above Various diseases are applied to the soil or plant body of cultivated plants, but the application method is not particularly limited, and is appropriately selected depending on the type of the disease or the type of the applicable plant. For example, the second control agent of the present invention can be mixed, dispersed or perfused with soil equivalent to cultivated plants, and the second control agent of the present invention can be applied. Direct application or spreading is equivalent to the plant body and the second control agent of the present invention is applied. Here, when applied to soil, the second control agent of the present invention may be applied to the soil to grow plants, and after planting to the soil, it may be applied to the soil. When dispersing the control agent of the second invention, the control agent of the second invention may be diluted with an appropriate amount of water and used. In addition, the application amount of the plant disease control agent of the second invention varies depending on the type of the disease #, the type of the applicable plant, etc., so it cannot be uniformly specified, but when it is dispersed and treated in the soil, it is converted into the filamentous spore concentration. Generally, 50 to 300 L of 104 to 106 cfu / ml of the control agent solution can be dispersed every 10a. [Embodiments] Examples Example 1 Hereinafter, the present invention will be described more specifically with reference to examples. (Isolated Penicillium waksmanii RU-1 5000 strain) Mango leaves were collected from mango cultivation farms around Okinawa Prefecture, and the filamentous fungi growing on the leaves were isolated from Rose Bengal medium described later. The isolated filamentous bacteria are stored in a PDA (Potato dextrose agar, potato glucose agar) medium. From the obtained strains, the strains having an antagonistic action against Colletotrichum gleosporiides are selected in the following manner. That is, The isolated bacteria were cultured on a PDA medium and cultured at 25 ° C for 4 days. A sterilized cork with a diameter of 5 mm was put through a -25- 200536481 (22) perforator, and the first end of the bacterial colony was perforated with the medium. The perforated flora was cultured in the PDA medium in the same way as the anthracnose colony obtained after the same culture. 5 days later, the radius of the anthracnose flora was measured, and the strain that inhibited the anthracnose of mango was selected. Thus, Penicillium Waksmanii RU — 15000 strain. (Composition of rose red medium) φ protein gland: 5g; monopotassium phosphate: 0.5g; dipotassium phosphate: 0.5g; magnesium sulfate heptahydrate: 0.5g; glucose: 10g; yeast extract : 0.5g; rose red: 0.05g; streptomycin sulfate: 0.03g; agar: 20g; distilled water: 1000ml Example 2 (culture Penicillium waksmanii) (1) solid culture Penicilliu m waksmanii RU — 15000 strain spores, cultured in a PDA medium at 25 ° C for 5 days, and then inoculated with platinum ears to potato dextrose broth ', and cultured under shaking at 25 ° C. Secondly, in a sterilized bran prepared with a moisture content of 50% by weight, after inoculating the PD liquid medium in the mixed portion, 'the solid stationary culture was started at 25 ° C. After 7 days of culture, when counting the number of spores, 1 x 100 spores are formed per 1 g of bran. After drying the bran, a large number of spores of strain RU-15,000 are obtained through sieving. -26- 200536481 (23) (2) Liquid culture

Penicillium waksmanii RU — 15000 strain was cultured in a PDA medium at 25 ° C for 5 days, and then a portion of the white fungus was inoculated into a liquid medium using malt extract (manufactured by DIFCO) and shaken at 25 to cultivate. After 7 days of culture, when counting the number of spores in the culture medium, 1 X 108 spores were formed per 1 ml of the culture medium. This culture was filtered through gauze and centrifuged to obtain a large number of RU-15 000 spores. Example 3 (Preparation Example 1) The crushed product of Penicillium waksmanii RU — 15000 strain obtained from the solid culture in Example 2 (1) above! 〇g, the force D into 5g of the naphthyl naphthalene sulfonate as a surfactant and 8 5 g of viscosity minerals (made by Kuangguang Mining Co., Ltd .: K clay) as a carrier to prepare a hydrating agent. The spore concentration of this hydrating agent was 1 X 109 cfu / g. Example 4 (Confirmation of Control Effect on Strawberry Powdery Mildew) Two mother strawberry plants (variety: Fortunate Flower) were planted in a plastic cultivation pot of 20 × 60 cm to produce seedlings. They were successively bred in black plastic bowls with a diameter of 9 cm filled with soil. The test system prepared 6 plastic cultivation pots as described above. At this time, powdery mildew did not occur in strawberries in any of the pots. In the prepared plastic cultivation pots, the three strawberries in the plastic cultivation pots were scattered with tap water as the three groups in the untreated area. For the remaining -27-200536481 (24) strawberries in the remaining three plastic cultivation pots, the control agent of Formulation Example 1 was diluted by tap water to 100 times, and it was spread 6 times every 7 days. One week after the last spread, the incidence of powdery mildew that occurred naturally was investigated. First, on each leaflet of the three compound leaves above the strawberry seedling, the disease index was scored according to the degree of disease shown in Table 1.

Symptom index 0 No onset 1 The area ratio of the diseased area is less than 5% 2 The area ratio of the diseased area is more than 5%, 25% is less than 3 The area ratio of the diseased area is more than 25%, and the area ratio of 50% is less than 4 Based on this data, 50% or more of the diseased leaflet rate and the diseased rate defined by the following formula 1 were calculated. 〇Xnp + l Xnj + 2Xn2 + 3Xn3 + 4Xn4 X 1 〇 〇 Incidence rate (%)

4XN However, the total number of leaflets after N survey, η () to Π4 are the number of leaflets with an incidence index of 0 to 4-28- 200536481 (25) In addition, based on the incidence data, calculate as follows Control rate for non-treatment zone as defined in 2. Incidence rate in untreated area-Incidence rate in niece cloth area Control rate (%) = ----- X 1 0 0 Formula 2 Incidence rate in untreated area

The results are shown in Table 2.

Investigation Survey Incidence Incidence Control rate Number of trees Leaflet number Leaflet number Leaflet rate No treatment area 30 236 127 5 3.9% 30.4 Distribution area 30 228 9 3.9% 1. 2 96.1 As shown in the results of Table 2, RU — 1 5 000 The distribution area of the strains suppressed the occurrence of strawberry powdery mildew than the untreated area. Example 5 (Confirmation of control effect on strawberry anthracnose) When investigating the incidence of strawberry powdery mildew in Example 4, 'these strawberry seedlings, petioles, and leaves of strawberry anthracnose traces' were counted to confirm the number of strawberry anthracnose disease 'Calculate the diseased plant rate. Furthermore, based on the diseased plant rate data, a control rate as defined by the following formula 3 was calculated. -29- 200536481 (26) Formula 3 Control rate (%) Incidence rate in untreated area-Incidence rate in distribution area ------ X 1 0 0 Incidence rate in untreated area The results are shown in Table 3 As shown.

Survey number of diseased trees Number of diseased trees Rate of control rate No treatment area 60 9 15.0 _ Dispersion area 60 1 1.7 88.7 As shown in the results in Table 3, the RU — 15,000 strain distribution area is more effective than the non-treatment area in inhibiting strawberry anthracnose. Onset. φ Example 6 (Confirmation of the control effect on tea anthracnose) Yabuki 10-year raw tea was used, and the area was 30 m2. There was no response, and the tea was targeted for 3 times. The tea was picked twice on June 23, starting from July 1st, 100% sprouting. Every 4 days, 4 times, spreading 300 L / a of the control agent of Formulation Example 1 diluted with tap water. 〇〇〇 〇 Times. The untreated area is sprinkled with tap water to replace the control agent diluent. The number of diseased leaves of tea per lm2 of tea cultivation area was investigated on August 11 1 week after the final distribution and August 25 3 weeks after the final distribution, respectively. The diseased leaves were removed with each investigation -30-200536481 (27). Based on the number of diseased leaves, the control rate for the non-treatment zone as calculated by the following formula 4 was calculated. The number of diseased leaves in the untreated area-the number of diseased leaves in the distribution area Control rate (%) --- X 1 0 〇 4 The number of diseased leaves in the untreated area The results are shown in Table 4. Table 4

Number of diseased leaves (piece / m2) August 1 Number of diseased leaves (piece / m2) August 25th Total prevention rate No treatment area I 300 259 Π 422 228 m 2 13 92 Average 3 11.7 193.0 5 04.7 Distribution area I 25 4 1 Π 34 15 m 58 18 average 39.0 24.7 63.7 87.4 -31-200536481 (28) As shown in the results of Table 4, the spread of the RU — 15,000 strains' suppresses the incidence of charcoal scar disease. In addition, "from the results shown in Table 4, after about one month from the final distribution", the control agent of the present invention continued. Example 7 (Cultivation of filamentous bacteria) (1) Solid culture Penicillium waksmanii FERM BP-1 02 5 8 PDA medium was cultured at 25 ° C for 5 days, and then the white portion was placed in PD liquid medium at 25 After shaking culture at ° C overnight, a PD liquid medium containing 50% by weight of sterilized bran in water was prepared in advance, and then began to solid at 25 ° C. After 7 days of culture, spores were formed per 1 g of bran when counting the number of spores. After the bran is dried, the sieving machine can be used to recover a large number of 10 2 5 8 spores. (2) Liquid culture Penicillium waksmanii FERM BP-10258 medium was cultured at 25 ° C. for 5 days. Then, liquid culture medium using malt extract (manufactured by DIFCO) was connected to platinum ears and cultured by shaking. After 7 days of culture, 1 ml of the spores in the culture medium was counted to form 1 x 108 spores. This liquid was cultured and filtered, and then centrifuged to obtain a large amount of FERM ΒΡ — 1 025 8? Better than no treatment, even if the control effect

Spores are inoculated with gold ear. Secondly, when inoculated and mixed and cultured [X 1 010 FERM BP, the number of PDA seed parts was counted under 25, and gauze buns were used for each base. -32- 200536481 (29) Example 8 (Preparation Examples 2 to 4) 10 parts by weight of Penicillium waksmanii FERM BP — 1 02 5 8 spores and 5 parts by weight of polysaccharide recovered by the method of Example 7 (1) above Ethylene oxide based ether is a surfactant, and 85 parts by weight of a flowing paraffin emulsion is added (Formulation Example 2). Separately used instead of Lu PeniciUium waksmanii FERM BP-1 025 8

Talaromyces flavus FERM P-15816 &gt; Gliocladium virens FERM P- 1 73 8 1 and the same operation was performed to obtain an emulsion (respectively, formulation examples 3 and 4) 〇 The spore concentrations of formulation examples 2 to 4 were 50 0 1 0 9 c fu / g, 1 · 5 X 1 0 9 cfu / g, 3.0 X 1 08 cfu / g. In addition, the properties of the flowing paraffin used are 0.8 5 60 (15/4 ° C), dynamic viscosity is 7.98mm2 / s (40 ° C), and flash point is 160 ° C. • Example 9 (Confirmation of Control Effect on Strawberry Powdery Mildew) Strawberry seedlings (species: Fortune Flower) grown in plastic bowls were planted in a greenhouse on October 23. At this time, the width of the barnyard was 50 cm, the space between the barnyards was 50 cm, the space between the plants was 20 cm, and two rows were planted. The lowest temperature in winter is 12 ° C. Powdery mildew did not occur before the trial began. Starting from February 10, 4 times every other week, spreading 2 50 L of water-diluted preparation example 2 to 1,000 times per 10a, and about preparation example-33- 200536481 (30) 3 The same operation. As the non-treatment zone, tap water was used instead of the formulation example, and the same operation was performed. The above tests were performed in 3 groups of 24 trees in Zone 1. One week after the final spread (March 9), the incidence of powdery mildew that occurred naturally was investigated. The investigation was made up of 20 trees except 4 trees at both ends of the area, and the top 3 compound leaves of each leaflet. The diseased leaflet rate and incidence rate were obtained based on the following disease degree scores, and the incidence of untreated areas was obtained from the incidence rate. Control rate. On February 18, powdery mildew was first detected in the untreated area. Index 0; no incidence index 1; lesion area rate is 5% and under index 2; lesion area rate is above 5% and 25% is under index 3; lesion area rate is above 25% and 50% is under Index 4; The test results of the disease area ratio of 50% or more are shown in Table 5. • Incidence rate two Σ ((number of diseased leaflets X index) xl 00 / (investigation leaflet number) x4) Control rate two (1 Incidence rate in scattered areas / incidence rate in untreated areas) xl 00 See Table 5 The results showed that Penicillium waksmanii FERM BP-10258 distribution area and Talaromyces flavus FERM P-15816 distribution area suppressed the incidence of powdery mildew compared with the untreated area. Example 1 〇-34- 200536481 (31) (Confirmation of the control effect on tomato leaf mold) The Fanjie seedling (variety: Momotaro) will be bloomed in the first flower room on May 12, with a width of 100 cm 50 cm between the plants were planted in the greenhouse. No leaf mold occurred before the trial began. On May 29, June 5, and June 12, 150 L of 10 to 100 times the amount of diluted preparation example 2 with tap water was distributed every 10a. The same operation was performed for preparation example 3. In addition, tap water # was used as the untreated zone instead of the formulation example, and the same operation was performed. The above test was performed in 3 groups of 12 trees in zone 1. On June 19th, the number of diseased spots was investigated for 10 compound leaves before and after the second fruit house of 10 trees except the two ends of each district. The incidence rate, control rate, and the like were calculated in the same manner as in Example 9. The results are shown in Table 6.

Investigate the number of diseased spots * Control rate Number of plants Number of plants Penicillium (preparation example 2) 30 9 30 6.2 79.7 Talaromyces (preparation example 3) 30 9 30 6.2 79.7 No treatment area 30 30 100 30.5 per 100 compound leaves such as The results in Table 6 show that compared with the untreated area, pen 丨 c 丨 丨 丨 丨 um waksmanii FERM BP — 10258 distribution area and Talaromyces flavus -35- 200536481 (32) FERM P-15816 distribution area inhibited tomato leaf mold Onset. Example 11 (Confirmation of control effect on anthracnose of tea) Yabuki 10-year-old green tea was used, and the area was 30 m2. There was no response, and three times of tea was used. In preparation for growth, pruning will be carried out on July 25. From August 6 every 7 days, 4 times, the preparation example 20 was diluted with tap water to 200,000 times, and 300 L was dispersed every 10 a, and the same operation was performed for the preparation example 3. The same operation was performed by using tap water as the untreated zone instead of the formulation example. On September 17th, 3 weeks after the final distribution, the number of diseased leaves in 3 locations per 1 m2 was investigated. The results are shown in Table 7.

-36-200536481 (33)

Number of hair spots (pieces / m2) Control rate Penicillin zone I 12 (Preparation Example 2) Π 56 m 50 Average 39.3 87.5 Talaromy ces I 46 (Preparation Example 3) Π 60 m 40 Average 48.7 84.4 No treatment zone I 25 1 Π 262 m 430-average 3 14.3 As shown in the results of Table 7, compared to the untreated area: Penicillium waksmanii FERM BP-10258 distribution area and Talaromyces flavus FE RM P -1 5 8 1 6 Onset. Example 12 2 (Confirmation of the control effect of onion white silkworm disease) The seedlings of onion (variety: Tokyo winter black) sown on March 25th were taken on May 19th, with a plant space of 2. 7 cm and a row space of 1 m colonization. Started the test -37- 200536481 (34) Onion white silkworm disease did not occur before the test. Among the 4 times of soil composting on June 23, July 14, August 11 and September 22, the first 2 times of soil composting will be diluted with tap water to a factor of 500. m2 spreads 0 · 5 L and deals with the surroundings of the roots. In addition, tap water was used as the untreated E system instead of the formulation example, and the same operation was performed. This test was performed in 3 groups of 20 m2 (5 X 4 m) in zone 1. Onion was excavated on October 6 to measure the onion 2m in the center of the test area, and the roots were investigated. • The onion strains with hyphae attached to the nucleus and leaf sheaths were counted, and the diseased strains were counted. The results are shown in Table 8.

Surveyed number of diseased trees Control rate Mumycin area I 82 3.7 (manufacturing example 4) Π 91 8.8 m 85 4.7 average 86.0 5.7 76.2 no treatment area I 86 25.6 Π 84 26.2 m 8 1 19.8 average 85.0 23.9 as shown in Table 8 The results show that, compared with the untreated area, the G 1 iocc adium virens FERM P-1 73 8 1 distribution area inhibited the incidence of onion white silkworm disease. -38- 200536481 (35) Example 13 (preservation stability test) The method of Example 7 (1) above was used to recover Penici 11 ium waksmanii FERM BP — 10258, Talaromyces flavus FERM P-15816 ^ Gliocladium Wrens FERM P -17381 conidia powder. These conidial powders and the preparations 2 to 4 described in Example 8 were used to perform storage stability tests as described below. φ The above conidia powder and preparation examples 2 to 4 were placed in an incubator at 35 ° C, and the survival rate of each spore was investigated over time. The spore survival rate was determined by diluting each preparation and conidia powder 10-fold with a 0.05% Tween20 (surfactant) aqueous solution after sterilization to grow the bacteria on a potato glucose agar culture medium to obtain the number of bacteria. The results are shown in Table 9.

-39- 200536481 (36) Table 9 Spore survival rate (%) 3 Days elapsed at 5 ° C 0 14 30 Penicillium conidia 100 65 20 Formulation example 2 (penicillium) 100 80 55 Trichoderma conidia 100 80 30 Formulation Example 3 (Trichoderma) 100 80 65 Gliocladium mold conidia 100 70 20 Formulation Example 4 (Penicillium) 100 80 60 As shown in the results of Table 9, it is shown that the formulation of the present invention is a storage stability tube-40-

Claims (1)

200536481 (1) 10. Scope of patent application 1. A strain characterized by having antagonistic effects on plant pathogens and belonging to Penicilliumwaksmanii. 2. — Penicillium waksmanii FERM BP — 10258 strain or a mutant thereof, which is characterized by its antagonistic effect on plant pathogens. 3. A plant disease control agent, which is characterized in that it contains bacterial cells such as the strain or mutant of item 1 or 2 of the patent application scope. φ 4 · The plant disease control agent according to item 3 of the patent application scope, wherein the plant disease is one or more than one selected from the group consisting of strawberry powdery mildew, mango anthracnose, strawberry anthracnose, and tea anthracnose. 5. A method for controlling plant diseases, which is characterized in that a plant disease control agent such as the item 3 or 4 of the scope of patent application is applied to the soil or plant body of cultivated plants. 6-A plant disease control agent, which is characterized by containing filamentous spores that have an antagonistic effect on plant pathogenic bacteria and mineral oil that does not cause the spores to have a poor survival. 7. The plant disease control agent according to item 6 of the application, wherein the mineral oil is a white mineral oil. 8. The plant disease control agent according to item 6 of the application, wherein the mineral oil is a mobile paraffin refined by hydrogenation. 9. The plant disease control agent according to item 6 of the application, wherein the mineral oil is hydrogenated, and then the purified paraffin is purified by sulfuric acid washing. 10. The plant disease control agent according to item 6 of the patent application, wherein the filamentous fungus having an antagonistic effect on plant pathogens belongs to the genus Penicillium (-41-200536481 (2) Penicillium), Talaromyces, and Gliocladium (Gliocladium) or Trichoderma (Trichoderma) filamentous fungi. 1 1. The plant disease control agent according to item 6 of the patent application scope, wherein the filamentous fungal line Penicillium waksmanii, Talaromyces fl avus, Gliocladium virens, or Trichoderma virens o 1 2 which has an antagonistic effect on plant pathogens The plant disease control agent of the sixth item, wherein φ is a filamentous fungus Penicillium waksmanii FERM BP— 1 025 8, Talaromyces flavus FERM P-15816, Gliocladium virM FERM P- 1 73 8 1, which has an antagonistic effect on plant pathogens. Trichoderma virens ATCC13213, Trichoderma virens ATCC24290 or a mutant thereof. 1 3. The plant disease control agent according to item 6 of the patent application, wherein the spores are conidia, ascospores, or thick film spores. 1 4. The plant according to any one of items 6 to 13 of the scope of patent application Φ Disease control agent, which further contains silicon dioxide. 15. A method for controlling plant diseases, which is characterized in that a plant disease control agent according to any one of claims 6 to 14 is applied to the soil or plant body of a cultivated plant. 16. A method characterized in that the conidia of the filamentous fungus are recovered from the cultured bacterial body with white mineral oil. -42- 200536481 VII Designated representative: (1) The designation of the designated representative in this case is: None (2) The component representative symbol of the representative: Brief description: None 8. If there is a chemical formula in this case, please disclose the chemical formula that best shows the characteristics of the invention: None