JPH0591869A - Plant pathogenic fungus-inhibiting microorganism and its utilization - Google Patents

Plant pathogenic fungus-inhibiting microorganism and its utilization

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Publication number
JPH0591869A
JPH0591869A JP3255099A JP25509991A JPH0591869A JP H0591869 A JPH0591869 A JP H0591869A JP 3255099 A JP3255099 A JP 3255099A JP 25509991 A JP25509991 A JP 25509991A JP H0591869 A JPH0591869 A JP H0591869A
Authority
JP
Japan
Prior art keywords
bacillus
soil
growth
disease
plant pathogenic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP3255099A
Other languages
Japanese (ja)
Other versions
JPH0734738B2 (en
Inventor
Yuzo Kinooka
雄三 紀岡
Hiroaki Masumura
弘明 増村
Katsunori Noguchi
勝憲 野口
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
YUUKISHITSU HIRYO SEIBUTSU KAS
YUUKISHITSU HIRYO SEIBUTSU KASSEI RIYOU GIJUTSU KENKYU KUMIAI
Original Assignee
YUUKISHITSU HIRYO SEIBUTSU KAS
YUUKISHITSU HIRYO SEIBUTSU KASSEI RIYOU GIJUTSU KENKYU KUMIAI
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Priority to JP3255099A priority Critical patent/JPH0734738B2/en
Publication of JPH0591869A publication Critical patent/JPH0591869A/en
Publication of JPH0734738B2 publication Critical patent/JPH0734738B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Abstract

PURPOSE:To obtain the subject microorganism effective for the ecological control of plant pathogenic fungi by separating and screening microorganismic strain from Bacillus which inhibits the growth of the plant pathogenic fungi from soil. CONSTITUTION:Microorganismic strain belonging to the genus Bacillus having strong antagonistic properties against the Fusarium fungi, the Phytopathola fungi, etc., are separated and screened to obtain the objective Bacillus SP BS-0001 and Bacillus SP BS-0001-SMCP III. The obtained microorganism is added to or mixed with seeds, roots and leaves of plants or with soil.

Description

【発明の詳細な説明】Detailed Description of the Invention

【0001】[0001]

【産業上の利用分野】本発明は、植物病原菌病の制御に
有効な微生物及び該微生物の生態的防除への利用法に関
する。
BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microorganism which is effective in controlling plant pathogens and a method of using the microorganism for ecological control.

【0002】[0002]

【従来の技術】農業及び園芸において、土壌伝染性病害
が重要な問題であり、なかでも野菜、花、果樹などのフ
ザリウム菌による萎凋病、つる割れ病、半枯病、萎黄
病、乾腐病などや、フィトパソラ菌やピシウム菌、リゾ
クトニア菌による根腐病、疫病、苗立枯病などや、バー
ティシリウム菌による半身萎凋病、黄化病、また、白紋
羽病菌、紫紋羽病菌による果樹、野菜の紋羽病、トマト
やナスの青枯病などが大きな問題となっている。
2. Description of the Related Art Soil-borne diseases are important problems in agriculture and horticulture, and among them, wilt disease, creeping vine disease, half-blight disease, yellow rot disease, and dry rot disease caused by Fusarium bacteria such as vegetables, flowers and fruit trees. , Etc., root rot, plague, seedling blight caused by Phytopasora, Pythium, Rhizoctonia, etc. Fruit tree, vegetable crotch disease, and bacterial wilt of tomato and eggplant are major problems.

【0003】また、空中伝染性病害においても、うどん
こ病、灰色カビ病などの病気が重要な問題である。農業
において、作物の安定生産のためにはこれら病害を回避
することは最重要課題である。これら病害を回避するた
めの手段としては、現在、抵抗性品種の利用や、薬剤消
毒が主を占めている。
Also in airborne diseases, diseases such as powdery mildew and Botrytis cinerea are important problems. In agriculture, avoiding these diseases is the most important issue for stable production of crops. As means for avoiding these diseases, use of resistant varieties and chemical disinfection are currently the main means.

【0004】しかしながら、これらの方法だけでは病害
からの回避は困難である。一方では環境、公害、健康問
題から、生産物の安全性に対する見方が厳しくなり、ま
た、世界的にも環境問題がクローズアップされ、環境保
全、生態的調和型農業が求められる情勢下にある。こう
いった観点から、拮抗微生物を用いた病害防除の試みが
数多くなされている。
However, it is difficult to avoid diseases by these methods alone. On the other hand, environmental, pollution, and health issues have led to a stricter view on the safety of products, and environmental issues are being highlighted worldwide, and there is a need for environmental conservation and ecologically harmonized agriculture. From such a viewpoint, many attempts have been made to control diseases using antagonistic microorganisms.

【0005】綿のリゾクトニア・ソラニ (Rhizoctonia
solani) 汚染土で綿をシュードモナス・フルオレッセン
ス (Pseudomonas fluorescens)を種子処理することによ
り、生存率が高まったとの報告がある[シー・アール・
ホーウェル 他:ファイトパソロジー (C.R.HOWELL et
c. : Phytopathology) Vol.69, No.5, 1979年]。カー
ネーションのフザリウム菌 (Fusarium oxysporum f. s
p. dianthi)による萎凋病に対してアルカリゲネス・エ
スピー (Alcaligenes sp.)の菌懸濁液にカーネーション
の苗を浸漬することにより萎凋病防除の可能性を示した
[ジー・ワイ・ユウエン 他:ファイトパソロジー (G.
Y.YUEN etc. : Phytopathology) Vol.76, No.2, 1986
年]。
Rhizoctonia on cotton
(solani) Seed treatment of cotton with Pseudomonas fluorescens in contaminated soil has been shown to increase survival rates [see
Howell et al .: Fight Pathology (CRHOWELL et
c .: Phytopathology) Vol.69, No.5, 1979]. Fusarium oxysporum f. S of carnation
against the wilt disease caused by p. dianthi, we showed the possibility of controlling the wilt disease by soaking carnation seedlings in a bacterial suspension of Alcaligenes sp. [G.Y.Yuyuan et al .: Fight Pathology (G.
Y.YUEN etc .: Phytopathology) Vol.76, No.2, 1986
Year].

【0006】キクのリゾクトニア・ソラニ (Rhizoctoni
a solani) に起因するキク茎くされ病の防除に拮抗菌グ
リオクラディウム・デリクエッセンス(Gliocladium del
iqu-escens)、トリコデルマ・エスピーピー (Trichode
rma spp.)、バチルス・エスピーピー (Bacillus spp.)
などを用いて試験を行っている[陳昇明 他:日本菌学
会報告,29, 1988年]。
Rhizoctoni of chrysanthemum
a solani) for the control of chrysanthemum stem disease caused by the antagonist Gliocladium delique essence (Gliocladium del
iqu-escens), Trichoderma spp (Trichode
rma spp.), Bacillus spp.
Etc. are used to test [Chen Sheng Ah et al .: Report of the Mycological Society of Japan, 29, 1988].

【0007】ダイコンのリゾクトニア・ソラニ (Rhizoc
tonia solani) による苗立枯病菌の抑制にシュードモナ
ス・セパシア(Pseudomonas cepacia)の種子バクテリゼ
ーションにより効果を認めている[本間善久 他:日本
植物病理学会報告,55, 1989年]。レタスのピシュウム
・ウルテイマム (Pythium ultimum)による病気に対し
て、エンテロバクター・クロアセ (Enterobacter cloac
ae) によるバイオコントロールの試みの報告がある[デ
イ・アール・フラベル 他:プラント・アンド・ソイル
(D.R.FRAVEL etc. : Plant and Soil)、125, 1990
年]。
Rhizoc of the Japanese radish
The effect of seed bacteriostasis of Pseudomonas cepacia on the inhibition of bacterial wilt disease caused by P. tonia solani has been confirmed [Yoshihisa Honma et al., Report of the Japanese Society for Plant Pathology, 55, 1989]. Enterobacter cloacé (Pythium ultimum) against lettuce
ae) has reported a biocontrol attempt [D.R. Fravel et al .: Plant & Soil]
(DRFRAVEL etc .: Plant and Soil), 125, 1990
Year].

【0008】豆のスクレロテューム・ロルフシ (Sclero
tiumrolfsii) による病気の綿のリゾクトニア・ソラニ
(Rhizoctonia solani) による病気をキチン分解活性を
もつセラティア・マルセッセンス (Serratia marcescen
s)で病害を防除する試験を行っている[アイ・チェット
他:プラント・アンド・ソイル (I.CHET etc. : Plan
t and Soil), 129 1990年] こういった拮抗微生物を利用した生態的病害防除技術
は、今後ますます重要になるものと考えられる。
[Sclerothum Lolfushi]
tiumrolfsii) diseased cotton Rhizoctonia solani
Diseases caused by (Rhizoctonia solani) have a chitin-degrading activity (Serratia marcescen)
s) are conducting tests to control disease [I.CHET etc .: Plan
t and Soil), 129 1990] Ecological disease control technology using such antagonistic microorganisms is expected to become more important in the future.

【0009】[0009]

【発明が解決しようとする課題】本発明は、農業上、重
要な問題である植物病害の制御に有効な方法であり、生
態系調和型農業への利用場面を提供するものである。
The present invention is an effective method for controlling plant diseases, which is an important problem in agriculture, and provides a situation of application to eco-friendly agriculture.

【0010】[0010]

【課題を解決するための手段】本発明者らは、トマト萎
凋病菌、キュウリつる割れ病菌、メロンつる割れ病菌、
ダイコン萎黄病菌、イチゴ萎黄病菌などのフザリウム属
菌、苗立枯病、疫病、根腐れ病のフィトパソラ属菌、ピ
シウム属菌、リゾクトニア属菌、トマト半身萎凋病、ハ
クサイ黄化病などのバーティシリウム属菌、白紋羽病を
起こすロゼリニア属菌、紫紋羽病の原因であるヘリコバ
シディウム属菌などの植物病原菌に拮抗作用を示す菌を
検索し、その利用法を鋭意研究した。
[Means for Solving the Problems] The present inventors have found that wilt fungus of tomato, cucumber creeping fungus, melon creeping fungus,
Fusarium such as radish yellow rot, strawberry yellow rot, etc., seedling blight, plague, root rot phytopasora genus, Pythium genus, Rhizoctonia genus, tomato half wilt, Chinese cabbage yellow disease and other verticillium We searched for fungi that have antagonism against plant pathogens such as genus fungi, Rosella genus that causes white rot, and Helicobacter bacillus that causes purple rot, and studied how to use them.

【0011】その結果、土壌中から各種病原菌に強い拮
抗性を示すバチルス属菌を分離、選抜し、バチルス・エ
スピー (Bacillus sp.) BS−0001(微工研菌寄12534
号) を得た。この菌株の同定結果は以下のとおりであ
る。 1) 形態 (1) 細胞の形及び大きさ 0.8×2〜3μmの桿菌 (2) グラム染色法 陰性 (3) 胞子の有無・形 有り、楕円・細胞の中心に形成し、胞子のうはふくらむ (4) 運動性の有無 有り 2) 生理学的性質 (1) 嫌気的性質 生育しな
い (2) V−P反応 − (3) Egg-Yolk反応 + (4) 最高生育温度 45℃ (5) pH5.7培地での生育 + (6) ニュートリエント・ブロースでの生育 + (7) NaCl (5〜10%) 培地での生育 − (8) 糖類からの酸生成 a. グルコース + b. アラビノース − c. キシロース + d. マンニトール + (9) デンプンの加水分解 + (10) カゼインの分解 + 3) DNA中のG+C含量 53.2 以上の菌学的性質から本菌株はバチルス属に分類され
る。
As a result, Bacillus sp. Having a strong antagonistic activity against various pathogens was isolated and selected from the soil, and Bacillus sp. BS-0001 (Microorganisms Research Institute 12534) was selected.
No.) The identification results of this strain are as follows. 1) Morphology (1) Cell shape and size 0.8 × 2-3 μm bacilli (2) Gram staining method Negative (3) Presence or absence of spores, shape, spores formed in the center of cells and sporangia Is swollen (4) With or without motility 2) Physiological properties (1) Anaerobic properties No growth (2) VP reaction- (3) Egg-Yolk reaction + (4) Maximum growth temperature 45 ° C (5) Growth in pH 5.7 medium + (6) Growth in nutritive broth + (7) Growth in NaCl (5-10%) medium- (8) Acid production from sugars a. Glucose + b. Arabinose- c. Xylose + d. Mannitol + (9) Hydrolysis of starch + (10) Decomposition of casein + 3) G + C content in DNA This strain is classified into the genus Bacillus based on the mycological properties of 53.2 or higher.

【0012】類縁菌としてはバチルス・マセランス (Ba
cillusmacerans)が挙げられるが、バチルス・マセラン
スは胞子の形成位置が細胞の端であり、嫌気的生育を行
ない、アラビノースからの酸生成を行ない、カゼインを
分解しない点から本菌株とは異なり、本菌株はバチルス
属に属する新菌種である。また、バチルス・エスピー
(Bacillus sp.)BS−0001( 微工研菌寄第12534号) を
ニトロソグアニジン処理してクロラムフェニコール5pp
mに耐性を持つ新規菌株バチルス・エスピー (Bacillus
sp.) BS−0001-SMCPI III( 微工研菌寄第12516号) を
得た。本菌株は、クロラムフェニコールに耐性を持つ以
外は、バチルス・エスピー (Bacillus sp.) BS−0001
と同様の菌学的性質を有していた。本発明は、また、前
記新規微生物の生態的防除への利用法にも関するもので
ある。
Bacillus macerans (Ba
cillus macerans), but Bacillus macerans differs from this strain in that it has spore formation at the cell edge, undergoes anaerobic growth, produces acid from arabinose, and does not decompose casein. Is a new strain belonging to the genus Bacillus. Also, Bacillus SP
(Bacillus sp.) BS-0001 (Microtechnology Research Institute, No. 12534) was treated with nitrosoguanidine to give chloramphenicol 5 pp.
A new strain of Bacillus sp.
sp.) BS-0001-SMCPI III (Microtechnology Research Institute, No. 12516). This strain is Bacillus sp. BS-0001 except that it is resistant to chloramphenicol.
It had similar bacteriological properties to. The present invention also relates to a method of using the novel microorganism for ecological control.

【0013】かかる本発明の利用法においては、バチル
ス・エスピー (Bacillus sp.) BS−0001( 微工研菌寄
第12534号) 又はBS−0001-SMCPI III( 微工研菌寄第1
2516号 )を各作物種子に処理したり、養液栽培におい
て、養液へ添加したり、ロックウールマットや土耕栽培
において株元に灌注することもできる。また、本菌株を
バーミキュライトやゼオライト、木炭などの多孔質資材
に吸着したり、なたね油粕や蒸製骨粉、魚粕などの基質
に培養したり、基質と多孔質資材の混合物に培養し、そ
の培養物を播種床に使ったり、育苗培土に混合したり、
畑に施用することもできる。この基質や多孔質資材は本
菌株の生育を阻害しないものであれば特に限定されな
い。
In the method of using the present invention, Bacillus sp. BS-0001 (Microtechnological Research Institute No. 12534) or BS-0001-SMCPI III (Microtechnical Research Prof. No. 1
No. 2516) can be applied to each crop seed, added to the nutrient solution in the hydroponic culture, or irrigated to the base of the plant in the rockwool mat or soil culture. In addition, this strain is adsorbed on a porous material such as vermiculite, zeolite, charcoal, cultivated on a substrate such as rapeseed meal, steamed bone meal, fish meal, or a mixture of a substrate and a porous material, and the culture Can be used as a seed bed, mixed with seedling cultivation soil,
It can also be applied to the field. The substrate and the porous material are not particularly limited as long as they do not inhibit the growth of this strain.

【0014】また、うどんこ病、葉カビ病、灰色カビ病
などの空中伝染性糸状菌病に対して、本菌株の培養物を
葉面散布することもできる。また、本菌株は他の有効菌
と混合、併用することもできる。
In addition, the culture of this strain can be applied to the foliar surface against airborne infectious fungal diseases such as powdery mildew, mildew and gray mold. Further, this strain can be mixed and used in combination with other effective bacteria.

【0015】[0015]

【実施例】以下、実施例により本発明を更に詳細に説明
するが、本発明の範囲はこれらの実施例に限定されるも
のではない。
The present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited to these examples.

【0016】[0016]

【実施例1】本発明に係わる微生物BS−0001-SMCP II
Iをペプトン0.5g、酵母エキス0.3g、肉エキス0.3g、グ
ルコース1.0g、蒸留水 100ml、pH7.0 の液体培地を用い
て、30℃で72時間振盪培養を行った。この培養液にキュ
ウリ (南進:タキイ種苗) の種子を27℃で24時間浸漬、
催芽し、 1/2単位水耕液 (NO3-N:9me/l, NH4-N:0.7me
/l, Ur-N :0.3me/l, P:2.5me/l, K:4.15me/l, Mg:
1.9me/l, S:2.8me/l,Ca:4.65me/l, Mn:0.6ppm, B:
0.225ppm, Fe:1.85ppm)を含浸したロックウール播種マ
ットに播種し、温室内で9日間、二葉展開まで育苗し、
9cm×9cmのロックウール育苗マットに移植した。
Example 1 Microorganism according to the present invention BS-0001-SMCP II
Using a liquid medium containing 0.5 g of peptone, 0.3 g of yeast extract, 0.3 g of meat extract, 1.0 g of glucose, 100 ml of distilled water and pH 7.0, I was shake-cultured at 30 ° C. for 72 hours. Soak the seeds of cucumber (southern: Takii seedling) in this culture for 24 hours at 27 ℃.
Germination, 1/2 unit hydroponic solution (NO 3 -N: 9me / l, NH 4 -N: 0.7me
/ l, Ur-N: 0.3me / l, P: 2.5me / l, K: 4.15me / l, Mg:
1.9me / l, S: 2.8me / l, Ca: 4.65me / l, Mn: 0.6ppm, B:
0.225ppm, Fe: 1.85ppm) was sowed on a rockwool sowing mat impregnated with the same and grown in a greenhouse for 9 days until two-leaf development,
It was transplanted to a 9 cm × 9 cm rock wool seedling mat.

【0017】移植1週間後にキュウリつる割れ病菌 (Fu
sariumoxysporum f.sp. cucumerinumIFO 6384) の胞子
懸濁液を育苗マットに108/10ml 接種した。キュウリつ
る割れ病菌の調製はポテト・デキストロース液体培地を
用いて27℃で7日間培養し、三重ガーゼでろ過し胞子液
を得た。育苗期間内は適時、上記 1/2単位水耕液を供給
した。対照として、種子を蒸留水中に27℃で24時間浸
漬、催芽したものを同様に処理した。試験は各区10連で
行ない、移植22日後の生育と発病を調査し、キュウリつ
る割れ病に対する生態的防除効果を比較検討した。その
結果を表1に示した。
One week after transplantation, the cucumber creeping vine fungus (Fu
sariumoxysporum f.sp. cucumerinumIFO 6384 with a spore suspension of) was 10 8 / 10ml inoculation in nursery mat. The cucumber vine cracking fungus was prepared by culturing in a potato dextrose liquid medium at 27 ° C. for 7 days and filtering with triple gauze to obtain spore fluid. During the nursery period, the above 1/2 unit hydroponic solution was supplied at appropriate times. As a control, seeds were soaked in distilled water at 27 ° C. for 24 hours and germinated, and then treated in the same manner. The test was conducted in 10 stations in each section, and the growth and disease on the 22nd day after transplantation were investigated, and the ecological control effect against cucumber vine cracking disease was compared and examined. The results are shown in Table 1.

【0018】[0018]

【表1】 [Table 1]

【0019】BS−0001-SMCP III菌の種子処理によ
り、キュウリつる割れ病の発病が抑制され、地上部重が
重く、生育が優れる傾向にあった。
[0019] The seed treatment of BS-0001-SMCP III bacterium tended to suppress the development of cucumber vine cracking disease, the above-ground weight was heavy, and the growth was excellent.

【0020】[0020]

【実施例2】生骨粉500g、バーミキュライト500gをオー
トクレーブ殺菌し、これに本発明に係わる微生物BS−
0001-SMCP IIIを実施例1と同様な方法で液体培養した
培養物10mlと殺菌水300ml を添加、混合して5日間培養
し、40℃以下で通風乾燥して水分15%の試験試料を得
た。
[Example 2] 500 g of raw bone powder and 500 g of vermiculite were sterilized by autoclave, and the microorganism BS-
0001-SMCP III was liquid-cultured in the same manner as in Example 1 and 10 ml of a culture and 300 ml of sterilized water were added and mixed, and the mixture was cultured for 5 days and dried under ventilation at 40 ° C or lower to obtain a test sample having a water content of 15%. It was

【0021】この試験試料を育苗培土 (窒素 200mg、り
ん酸 1500mg、加里 200mg、苦土石灰4000mg/l)に2%添
加し、9.5cmポリポットに充填した。これに、バーミキ
ュライト床に播種し、二葉展開したキュウリ (南進:タ
キイ種苗) 苗を移植した。移植と同時にポテトデキスト
ロース液体培地で前培養しておいたキュウリつる割れ病
菌 (Fusarium oxysporum f.sp. cucumerinum IFO 6384)
の胞子懸濁液を1ポットに108/10ml 接種した。
2% of this test sample was added to nursery soil (200 mg of nitrogen, 1500 mg of phosphoric acid, 200 mg of potassium, 4000 mg / l of magnesia lime) and filled in a 9.5 cm polypot. Cucumber seedlings that had been sown on a vermiculite floor and expanded into two leaves (Southward: Takii seedling) were transplanted to this. Fusarium oxysporum f.sp. cucumerinum IFO 6384, which had been pre-cultured in potato dextrose liquid medium at the same time as transplantation
Spore suspension was inoculated 10 8/10 ml in 1 pot.

【0022】対照としてBS−0001-SMCP III培養資材
無添加の育苗培土を用い、3連で試験を行った。試験は
播種21日後、移植、病原菌接種15日後に発病度、地上部
重及び栽培土壌の微生物性を調査した。その結果を表2
に示した。
As a control, using BS-0001-SMCP III culture material-free seedling-raising soil, a test was conducted in triplicate. In the test, 21 days after sowing, 15 days after transplantation and inoculation of pathogenic bacteria, disease severity, aboveground weight, and microbial properties of cultivated soil were investigated. The results are shown in Table 2.
It was shown to.

【0023】[0023]

【表2】 [Table 2]

【0024】BS−0001-SMCP III培養資材を育苗培土
に添加することにより、BS−0001-SMCP III菌が土壌
中で増殖し、F.oxysporum 菌数を減少させており、その
結果、キュウリつる割れ病が抑制され、生育が優れる傾
向にあった。
By adding the BS-0001-SMCP III culture material to the nursery soil, BS-0001-SMCP III bacteria grow in the soil and the number of F. oxysporum bacteria is decreased. As a result, cucumber vine Cracking diseases were suppressed and growth tended to be excellent.

【0025】[0025]

【実施例3】トマト萎凋病土を1/5000aワグネルポッ
トに充填し、肥料をNとして15kg/10aとなるように有
機化成(8-8-8)を添加した。試験区にはBS-0001 を用
いて、実施例2と同様にして得た試験試料を500kg/10a
となるように添加した。これにトマト種子( 大型福寿:
サカタのタネ)を播種し、2カ月後に発病調査した。試
験は3連で行った。その結果を表3に示した。
Example 3 Tomato wilt soil was filled in a Wagner pot of 1 / 5000a, and an organic chemical compound (8-8-8) was added so that the amount of fertilizer was N and 15kg / 10a. BS-0001 was used for the test section, and a test sample obtained in the same manner as in Example 2 was used for 500 kg / 10a.
Was added. Tomato seeds (Large Fukuju:
Sakata seeds) were sown and the disease was investigated two months later. The test was performed in triplicate. The results are shown in Table 3.

【0026】[0026]

【表3】 [Table 3]

【0027】BS−0001培養資材を土壌に添加すること
により、トマト萎凋病の発病程度が抑制される傾向にあ
った。
The addition of the BS-0001 culture material to the soil tended to suppress the degree of tomato wilt disease onset.

【0028】[0028]

【実施例4】BS−0001を用い、実施例2と同様にして
得た試験試料を育苗培土( 窒素200mg、りん酸1500mg、
加里200mg、苦土石灰4000mg/l) に2%添加し、9.5cmポ
リポットに充填した。これに、バーミキュライト床に播
種し、二葉展開したメロン(プリンス:サカタのタネ)
苗を移植した。移植と同時にポテトデキストロース液体
培地で前培養しておいたメロンつる割れ病菌(Fusarium
oxysporum f.sp.melonis IFO 6385) の胞子懸濁液を1
ポットに108/10ml接種した。
[Example 4] Using BS-0001, a test sample obtained in the same manner as in Example 2 was used to raise seedlings (200 mg of nitrogen, 1500 mg of phosphoric acid,
2% was added to Kari 200 mg and magnesia lime 4000 mg / l), and the mixture was filled in a 9.5 cm polypot. The seeds were sown on a vermiculite floor and expanded into two leaves (Prince: Sakata seeds).
Transplanted seedlings. Melon vine cracking fungus (Fusarium) pre-cultured in potato dextrose liquid medium at the same time as transplantation
oxysporum f.sp.melonis IFO 6385) 1 spore suspension
It was inoculated with 10 8 / 10ml in the pot.

【0029】対照としてBS-0001 培養資材無添加の育
苗培土を用い、3連で試験を行った。播種41日後に発病
度と地上部重を調査した。その結果を表4に示した。
As a control, using BS-0001 culture material-free seedling-raising soil, tests were conducted in triplicate. 41 days after sowing, the severity and aboveground weight were investigated. The results are shown in Table 4.

【0030】[0030]

【表4】 [Table 4]

【0031】BS−0001培養資材を育苗培土に添加する
ことにより、メロンつる割れ病が抑制され、メロンの生
育が優れる傾向にあった。
By adding the BS-0001 culture material to the nursery soil, melon cracking disease was suppressed and melon growth tended to be excellent.

【0032】[0032]

【実施例5】ロックウール播種マットを用いて育苗した
トマト苗を二葉展開時にロックウール育苗キューブへ移
植した。移植時にYPMG液体培地を用いて増殖させた
BS-0001を1キューブ当り、108cell/5ml接種した。移
植2日後にトマト青枯れ病菌(Pseudomonas solanacearu
m MAFF 03-01843)を接種した。対照としてBS-0001無
接種区を用い、4連で試験を行った。
[Example 5] Tomato seedlings raised using a rockwool sowing mat were transplanted to rockwool seedling cubes when two leaves were developed. BS-0001 grown in a YPMG liquid medium at the time of transplantation was inoculated with 10 8 cells / 5 ml per cube. Two days after transplantation, Pseudomonas solanacearu
m MAFF 03-01843). The test was carried out in quadruplicate using the BS-0001 non-inoculated section as a control.

【0033】播種後31日間栽培し、発病度と生育を調査
した。その結果を表5に示した。
Cultivation was carried out for 31 days after sowing, and the degree of disease and growth were investigated. The results are shown in Table 5.

【0034】[0034]

【表5】 [Table 5]

【0035】BS−0001により、トマト青枯れ病の発生
が抑制され、トマトの生育が優れる傾向がみられた。
With BS-0001, the occurrence of bacterial wilt of tomato was suppressed and the growth of tomato tended to be excellent.

【0036】[0036]

【発明の効果】本発明によれば、植物病原菌による病気
を抑制し、作物を健全に育てることができる。
EFFECTS OF THE INVENTION According to the present invention, diseases caused by phytopathogenic fungi can be suppressed and healthy crops can be grown.

───────────────────────────────────────────────────── フロントページの続き (51)Int.Cl.5 識別記号 庁内整理番号 FI 技術表示箇所 C12R 1:07) ─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. 5 Identification code Office reference number FI technical display location C12R 1:07)

Claims (3)

【特許請求の範囲】[Claims] 【請求項1】 植物病原菌の生育を抑制する新規微生物
バチルス・エスピー(Bacillus sp.) BS−0001。
1. A novel microorganism Bacillus sp. BS-0001 which suppresses the growth of plant pathogens.
【請求項2】 植物病原菌の生育を抑制する新規微生物
バチルス・エスピー(Bacillus sp.) BS−0001-SMCP I
II。
2. A novel microorganism Bacillus sp. BS-0001-SMCP I that suppresses the growth of plant pathogens.
II.
【請求項3】 新規微生物バチルス・エスピー (Bacill
us sp.) BS−0001又はBS−0001-SMCP III を植物の
種子、根もしくは葉面又は土壌に処理することを特徴と
する該微生物の生態的防除への利用法。
3. A novel microorganism Bacillus sp.
us sp.) A method of using the microorganism for ecological control, which comprises treating BS-0001 or BS-0001-SMCP III on seeds, roots or leaves of plants or soil.
JP3255099A 1991-10-02 1991-10-02 Plant pathogen-suppressing microorganism and method of using the same Expired - Lifetime JPH0734738B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
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Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
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Publications (2)

Publication Number Publication Date
JPH0591869A true JPH0591869A (en) 1993-04-16
JPH0734738B2 JPH0734738B2 (en) 1995-04-19

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003046157A1 (en) * 2001-11-26 2003-06-05 Kumiai Chemical Industry Co., Ltd. Bacillus sp. d747 strain, plant disease controllring agents and insect pest controlling agents using the same and control method using the agents
WO2005082149A1 (en) * 2004-02-27 2005-09-09 Itsuki Co., Ltd. Method of controlling plant disease damage by using bacillus and controlling agent
JP2007055982A (en) * 2005-08-26 2007-03-08 Itsuki Co Ltd Method for controlling plant disease comprising trephocyte of bacterium of genus bacillus as active ingredient and controller
JP2007137888A (en) * 1997-12-08 2007-06-07 Koa Corp Agent for vegetation

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* Cited by examiner, † Cited by third party
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JPS62275677A (en) * 1986-05-23 1987-11-30 Lion Corp Microorganism belonging to bacillus genus
JPS63273470A (en) * 1987-01-21 1988-11-10 アグリカルチュラル、ジェネティックス、カンパニー、リミテッド Microorganism strain having antibacterial activity
JPH01132372A (en) * 1987-07-27 1989-05-24 Wisconsin Alumni Res Found Method for preventing fungicidal toxin, root rot disease and damping-off and inoculation material
JPH0248509A (en) * 1988-08-09 1990-02-19 Nippon Kayaku Co Ltd Method for controlling plant disease injury by kf-44 strain belonging to genus bacillus
JPH02209803A (en) * 1989-02-10 1990-08-21 Kureha Chem Ind Co Ltd Prevention of plant disease with bacterium
JPH02275898A (en) * 1989-01-10 1990-11-09 Mitsubishi Petrochem Co Ltd Novel antibiotic substance kt-6291a and kt-6291b, microorganism capable of producing the same, its production and plant disease injury controlling agent
JPH0377803A (en) * 1989-08-18 1991-04-03 Kao Corp Method for preventing soil disease injury

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS62275677A (en) * 1986-05-23 1987-11-30 Lion Corp Microorganism belonging to bacillus genus
JPS63273470A (en) * 1987-01-21 1988-11-10 アグリカルチュラル、ジェネティックス、カンパニー、リミテッド Microorganism strain having antibacterial activity
JPH01132372A (en) * 1987-07-27 1989-05-24 Wisconsin Alumni Res Found Method for preventing fungicidal toxin, root rot disease and damping-off and inoculation material
JPH0248509A (en) * 1988-08-09 1990-02-19 Nippon Kayaku Co Ltd Method for controlling plant disease injury by kf-44 strain belonging to genus bacillus
JPH02275898A (en) * 1989-01-10 1990-11-09 Mitsubishi Petrochem Co Ltd Novel antibiotic substance kt-6291a and kt-6291b, microorganism capable of producing the same, its production and plant disease injury controlling agent
JPH02209803A (en) * 1989-02-10 1990-08-21 Kureha Chem Ind Co Ltd Prevention of plant disease with bacterium
JPH0377803A (en) * 1989-08-18 1991-04-03 Kao Corp Method for preventing soil disease injury

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007137888A (en) * 1997-12-08 2007-06-07 Koa Corp Agent for vegetation
WO2003046157A1 (en) * 2001-11-26 2003-06-05 Kumiai Chemical Industry Co., Ltd. Bacillus sp. d747 strain, plant disease controllring agents and insect pest controlling agents using the same and control method using the agents
US7094592B2 (en) 2001-11-26 2006-08-22 Kumiai Chemical Industry Co., Ltd. Bacillus sp. D747 strain, plant disease controlling agents and insect pest controlling agents using the same and control method using the agents
CN100419070C (en) * 2001-11-26 2008-09-17 组合化学工业株式会社 Bacillus D747 strain, plant disease controlling agents or insect pest controlling agents using the same and control method using the agents
WO2005082149A1 (en) * 2004-02-27 2005-09-09 Itsuki Co., Ltd. Method of controlling plant disease damage by using bacillus and controlling agent
JP2007055982A (en) * 2005-08-26 2007-03-08 Itsuki Co Ltd Method for controlling plant disease comprising trephocyte of bacterium of genus bacillus as active ingredient and controller

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