JP3181847B2 - Microbial materials that control tomato-borne diseases of tomato - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microorganism for inhibiting the onset of the soil-borne disease "tomato root rot of tomato" of tomato, and its use. Related to the total system.

[0002]

2. Description of the Related Art Agricultural production, especially in profitable vegetable crops, is scaled up to overcome fierce competition between production areas.
Continuous cropping of single crops that are economically and laborably productive is increasing. Accumulation of pathogenic bacteria has been accelerated with continuous cropping, and continuous cropping disorders are intensifying. 19 conducted by the Ministry of Agriculture, Forestry and Fisheries Vegetable Experimental Station
According to a survey conducted in 1984, 60.8% of continuous cropping failures were caused by soil-borne pathogens, which are difficult to control diseases. (Ministry of Agriculture, Forestry and Fisheries Vegetable Experimental Station: Recent actual situation of continuous cropping failure of vegetables and flowers, research on vegetable experimental stations Document 18, 87-991984
Year).

Examples of soil-borne diseases include strawberry yellow blight, radish yellow blight, tomato root rot caused by Fusarium wilt, tomato blight, eggplant blight caused by bacterial wilt, and verticillium. There are major problems such as eggplant wilt disease and tomato wilt disease caused by disease-causing fungi, Chinese cabbage root-knot disease and cabbage root-knot disease caused by clubroot fungi. Avoiding these diseases is the most important issue for stable crop production.

[0004] As means for avoiding the disease, use of resistant varieties and resistant rootstocks, and fumigation disinfection of soil by chlorpicrin and methyl bromide are currently performed.

[0005] On the other hand, varieties that are good in fruit quality but are vulnerable to the disease may be selected, or resistant rootstocks with high seedling raising costs may not be used. In addition, fumigation of soil by chemicals may cause health problems for workers and destruction of the ozone layer by methyl bromide. Furthermore, there is a problem that it is difficult to control during the growth only by sterilization before tomato planting.

[0006] For this reason, attempts have been made to develop soil-control fungus-controlling microorganisms, ie, antagonistic microorganisms, that continuously suppress the onset of disease and to control disease using the microorganisms.

For example, Pseudomonas solanacearum OM2 is added to the root of a solanaceous plant.
Method to control bacterial wilt by inoculating bacteria (Japanese Patent Publication No. 8-1)
5428) and Pseudomonas SP (M) HR1
A method for controlling cabbage chlorosis, eggplant wilt and the like using 000 (Japanese Patent Laid-Open No. Hei 7-298874) has been proposed.

[0008] The technology for controlling ecologically soil-borne diseases using such antagonistic microorganisms is excellent in keeping the soil that is the basis of production healthy.

However, Japanese Patent Publication No. Hei.
The control of a soil-borne disease caused by Pseudomonas genus disclosed in -298874 is intended for bacterial wilt and the like, and does not relate to tomato root rot. On the other hand, the disease occupies the first place among soil-borne diseases of tomatoes occurring in Aichi Prefecture, for example (Heisei 5
Although it has caused serious damage, antagonistic microorganisms effective for the disease have not yet been developed, and their development is strongly expected.

[0010] Further, there is no established method of tracking bacterial strains after soil treatment using such microorganisms, or a method of detecting only used bacteria required for quality control of microorganism materials, etc. Has been questioned.

[0011]

DISCLOSURE OF THE INVENTION The present invention is an effective method for controlling the occurrence of soil-borne diseases which must be solved urgently in agricultural production, and provides a stable quality microbial material. The purpose is to propose a use scene for conservation agriculture.

[0012]

Means for Solving the Problems The present inventors have searched for bacteria that exhibit an antagonistic action against tomato root rot fungus and studied how to use them.

[0013] As a result, Pseudomonas spp., Which is antagonistic to the tomato root rot fungus, is isolated and selected from the rhizosphere of tomato, and Pseudomonas putida is isolated.
tida) AP-1 (Seikoken Bacteria FERM P-1582)
8) was obtained. The results of identification of this strain are as follows.

1) Morphology (1) Shape and size of cells Bacillus of 0.6 to 0.8 × 1.6 to 2.2 μm (2) Gram staining negative (3) Presence or absence of spores (4) Movement (5) Flagella form Polar flagella

2) Physiological properties (1) Aerobic properties Growing (2) Catalase reaction + (3) Oxidase reaction + (4) Acid production from glucose + (5) OF test O (sugar oxidatively (Decomposes) (7) Growing on MacConkey medium + (8) Growing at 10 ° C Growing (9) Growing at 37 ° C Growing (10) Reduction from nitric acid to nitrite-(11) Nitrogen to nitrogen -(12) Indole formation-(13) Nitrophenylgalacto-pyranoside (ONPG) reaction (14) Arginine hydrolase + (15) Lysine decarboxylase-(16) Use of ornithine-(17) Urea Hydrolysis-(18) Deamination of phenylalanine-(19) Utilization of anaerobic glucose-(20) Acid production from glucose + (21) Acid production from maltose + (22) Sucrose Acid generation-(23) Acid generation from D-xylose + (24) Hydrolysis of starch-(25) Utilization of citric acid + (26) Hydrolysis of esculin + (27) Hydrolysis of chitin-(28) Kato P -2 Production of Fluorescent Dye in Medium + This strain is classified as Pseudomonas putida from the above mycological properties.

As related bacteria, Pseudomonas putida
Pseudomonas putida biovar A and Pseudomonas putida biovar A
putida biovar B), but Pseudomonas
Both Petitida biovar A and Pseudomonas putida biobarbie can use ornithine and differ from this strain in that they deaminate phenylalanine.This strain was identified as a new strain belonging to Pseudomonas putida and FERMP added to Engineering Research Institute
Deposit No. -15828.

According to the present invention, the novel microorganism Pseudomonas putida AP-1 is further improved to obtain the antibiotic streptomycin resistant strain AP.
-1S1 (FERM P-16030) and the antibiotic rifampicin resistant mutant AP-1R1 (FERM P
-16031) and the antibiotic streptomycin / antibiotic rifampicin resistant mutant AP-1SR1 (FE
RM P-16032), which were deposited with the Life Science Research Institute. The properties of these mutant strains are as follows.

The antibiotic streptomycin resistant strain A
P-1S1 (FERM P-16030) 1) Morphology (1) Cell shape and size 0.6-0.8 × 1.6-2.2 μm bacilli (2) Gram staining negative (3) Spores (4) Motility available (5) Flagella form polar flagella

2) Physiological properties (1) Aerobic properties Growing (2) Catalase reaction + (3) Oxidase reaction + (4) Acid production from glucose + (5) OF test O (oxidizes sugars (Decomposes) (7) Growing on MacConkey medium + (8) Growing at 10 ° C Growing (9) Growing at 37 ° C Growing (10) Reduction from nitric acid to nitrite-(11) Nitrogen to nitrogen -(12) Indole formation-(13) Nitrophenylgalacto-pyranoside (ONPG) reaction (14) Arginine hydrolase + (15) Lysine decarboxylase-(16) Use of ornithine-(17) Urea Hydrolysis-(18) Deamination of phenylalanine-(19) Utilization of anaerobic glucose-(20) Acid production from glucose + (21) Acid production from maltose + (22) Sucrose Acid production + (23) Acid production from D-xylose + (24) Hydrolysis of starch-(25) Utilization of citric acid + (26) Hydrolysis of esculin + (27) Hydrolysis of chitin-(28) Kato P -2 Production of Fluorescent Dye in Medium + (29) Growth in Streptomycin 500 mg / l + Supplemented Medium Based on the above bacteriological properties, this strain AP-1S1 is capable of producing acid from sucrose and adding 500 mg of streptomycin. There is no difference from the AP-1 strain except for the resistance to / l.

The antibiotic rifampicin resistant strain AP-
1R1 (FERM P-16031) 1) Morphology (1) Cell shape and size 0.6-0.8 × 1.6-2.2 μm bacilli (2) Gram staining negative (3) Presence or absence of spores (4) Motility presence / absence (5) Flagella form polar flagella

2) Physiological properties (1) Aerobic properties Growing (2) Catalase reaction + (3) Oxidase reaction + (4) Acid generation from glucose + (5) OF test O (oxidizes sugars (Decomposes) (7) Growing on MacConkey medium + (8) Growing at 10 ° C Growing (9) Growing at 37 ° C Growing (10) Reduction from nitric acid to nitrite-(11) Nitrogen to nitrogen -(12) Indole formation-(13) Nitrophenylgalacto-pyranoside (ONPG) reaction (14) Arginine hydrolase + (15) Lysine decarboxylase-(16) Use of ornithine-(17) Urea Hydrolysis-(18) Deamination of phenylalanine-(19) Utilization of anaerobic glucose-(20) Acid production from glucose + (21) Acid production from maltose + (22) Sucrose Acid production + (23) Acid production from D-xylose + (24) Hydrolysis of starch-(25) Utilization of citric acid + (26) Hydrolysis of esculin + (27) Hydrolysis of chitin-(28) Kato P -2 Production of fluorescent dye in medium + (29) Growth in medium + supplemented with rifampicin 250 mg / l Based on the above mycological properties, this strain AP-1R1 performs acid production from sucrose,
Other than showing resistance to 0 mg / l, there is no difference from the AP-1 strain.

The antibiotic streptomycin / antibiotic rifampicin resistant strain AP-1SR1 (FERM P
-16032) 1) Morphology (1) Cell shape and size 0.6-0.8 x 1.6-2.2 µm bacilli (2) Gram staining negative (3) Presence or absence of spores No (4) Movement (5) Flagella form polar flagella

2) Physiological properties (1) Aerobic properties Growing (2) Catalase reaction + (3) Oxidase reaction + (4) Acid production from glucose + (5) OF test O (oxidizes sugars (Decomposes) (7) Growing on MacConkey medium + (8) Growing at 10 ° C Growing (9) Growing at 37 ° C Growing (10) Reduction from nitric acid to nitrite-(11) Nitrogen to nitrogen -(12) Indole formation-(13) Nitrophenylgalacto-pyranoside (ONPG) reaction (14) Arginine hydrolase + (15) Lysine decarboxylase-(16) Use of ornithine-(17) Urea Hydrolysis-(18) Deamination of phenylalanine-(19) Utilization of anaerobic glucose-(20) Acid production from glucose + (21) Acid production from maltose + (22) Sucrose Acid production + (23) Acid production from D-xylose + (24) Hydrolysis of starch-(25) Utilization of citric acid + (26) Hydrolysis of esculin + (27) Hydrolysis of chitin-(28) Kato P -2 Production of fluorescent dye in culture medium + (29) Growth in streptomycin 500 mg / l + supplemented medium (30) Growth in rifampicin 250 mg / l attached to ground + Due to the above mycological properties, this strain AP-1SR1 There is no difference from the AP-1 strain except for the acid production from sucrose and the resistance to streptomycin 500 mg / l and rifampicin 250 mg / l.

These antibiotic-resistant mutants may be obtained by isolation and breeding according to a conventional method. For example, the parent strain AP-1 may contain an antibiotic (50 to 500 mg / day).
1) It is sufficient to inoculate a selective medium and separate from the growing colony. Mutants are those obtained by mutating the parent AP-1 strain or those not mutating (natural mutation)
You can choose from

In order to obtain a mutant strain in the present invention, any known method may be used. Physical methods of mutation include ultraviolet irradiation, radiation irradiation, etc.
As a chemical method, there is a mutagenic agent, for example, a mutagenic method in which the agent is suspended in a solution of ethyl methanesulfonate, N-methyl-N-nitro-N-nitrosoguanidine, nitrite, acridine dye, and the like. Can be used as appropriate.

The strains AP-1S1 and AP-1R of the present invention
1 and AP-1SR1 have an effect of inhibiting the onset of soil-borne diseases, and can be easily and easily re-separated in plants or soil by utilizing the property of antibiotic resistance, and can be used for microbial materials. The quality control and the behavior of this strain in soil can be easily tracked and confirmed. In addition, even when the antibiotic is added to a nutrient solution or an irrigation solution for the purpose of sterilization, preservation, or the like, in the case of these antibiotic-resistant strains, the action and growth of antagonistic microorganisms are suppressed by the antibiotic. It is possible to prevent and control root rot of tomatoes without any problem.

In the method of using the strain of the present invention, seeds of a Solanaceae plant are treated, added to a nutrient solution in nutrient solution cultivation, and irrigated with a strain in rock wool mat or soil cultivation. Can also. In addition, this strain is adsorbed on porous materials such as perlite, vermiculite, charcoal, coffee extract cake, cultivated on substrates such as defatted soybean and bran, or cultured on a mixture of substrate and porous materials,
Compost can be inoculated or cultured, and the culture can be used for a sowing bed, mixed with seedling cultivation soil, or applied to soil. The substrate and the porous material are not particularly limited as long as they do not inhibit the growth of the strain.

Examples of the above-mentioned substrate and porous material include rice bran, beet meal, bagasse, corn meal, germ meal, peat moss, organic compost, rice straw, firgrass, expanded silica, diatomaceous earth, shell fossil, limestone, clay mineral (Kaolinite, acid clay, bentonite, montmorillonite, etc.) and others.

In order to produce a disease-inhibiting material according to the present invention, the bacterial strain of the present invention may be used as an active ingredient, mixed with a carrier or the like according to a conventional method, and made into a material.

[0030]

EXAMPLES The present invention will be described below in detail with reference to examples, but the scope of the present invention is not limited to these examples.

[0031]

Example 1 Antagonistic Bacteria AP-1, AP-1S1, AP-1R1, AP
-1SR1 Assay for Antagonistic Activity of Bacterial Wilt of Tomato Root suitable for tomato root rot is 15-20 ° C.
It occurs frequently at low soil temperatures from early winter to early spring in facility cultivation, and the disease progresses slowly at high temperatures, and may appear to have recovered in mildly ill strains (New Edition Soil Disease Guidebook: New Edition Soil Disease Guidebook, 32, 1984). Year). For this reason, the antagonistic microorganism must also be capable of growing in the above-mentioned range of the soil temperature (15 to 20 ° C.). The above ground temperature (15-20
° C) in the range (5, 10, 15, 20, 2,
5, 30, 35, and 40 ° C), the growth of antagonistic microorganisms and root rot fungi was investigated. That is, 1 according to the present invention
0 ⁸ / ml antagonistic bacteria AP-1, AP-1S1, AP-1R
1, 1 ml of AP-1SR1 and 1 ml of 10 ⁴ / ml root rot fungus 10 g of meat extract, 10 g of peptone, 5 g of sodium chloride, 15 g of agar, 1,000 ml of tap water, pH 7.0.
The meat extract agar medium of No. 1 was mixed and cultured 5 times in a petri dish into which aliquots were dispensed, and the growth ratio of antagonistic microorganisms and root rot fungi at each temperature was investigated, and compared with the growth of a single culture of root rot fungus. The results are shown in Table 1.

[0032]

[Table 1]

The above root rot fungus and antagonistic bacteria AP
-1 and AP-1 mutants, as evident from the results of the effects of temperature, on the root rot fungus (Fusarium oxysporu).
m f.sp.radicis-ricopersici) alone grows from 15 ° C. to 35 ° C., but antagonistic bacteria AP-1, AP-1
When S1, AP-1R1, and AP-1SR1 were mixed and cultured, they could grow only at 15 ° C. to 30 ° C., and their growth was also suppressed as compared with the single culture of root rot fungus. This indicates that the antagonistic bacterium AP-
1, AP-1S1, AP-1R1, and AP-1SR1 were determined to be usable.

[0034]

Example 2 Antagonistic Bacteria AP-1, AP-1S1, AP-1R1, AP
-1SR1 antibiotic resistance test Mutant strains AP-1S1 and AP-1R1 of antagonistic bacteria AP-1
And 10 g of meat extract, 2 g of yeast extract, 10 g of peptone, 2 g of sodium chloride, 15 g of agar, and 1,000 g of ion-exchanged water to confirm the antibiotic resistance of AP-1SR1 and AP-1SR1.
Streptomycin sulfate (S) 500 mg / l (NYS) or rifampicin (R) 250 mg / l (NYR) and streptomycin sulfate (S) 500 mg, rifampicin (R) 2
The growth of each antagonistic bacterium was investigated in a medium supplemented with 50 mg / l (NYSR). The results are shown in Table 2.

[0035]

[Table 2]

The above-mentioned antagonistic bacteria AP-1, AP-1S
1. As is clear from the results of the effects of antibiotics on the growth of AP-1R1 and AP-1SR1, the antagonistic bacterium AP-1 without antibiotic resistance did not grow on the medium supplemented with antibiotics. In addition, it was confirmed that the antibiotic-resistant antagonistic bacterial mutants AP-1S1, AP-1R1 and AP-1SR1 each grow in a medium exhibiting resistance.

[0037]

Example 3 Pot test of AP-1 against tomato root rot fungus In order to investigate the antagonistic effect of the antagonistic bacterium AP-1, soil 50 in which gray lowland soil and commercially available horticultural cultivation soil were mixed at a volume ratio of 1: 1 was used.
0 g was sterilized three times at 121 ° C. for 1 hour, filled in a polypot having a diameter of 12 cm, and cultivated in a pot with a PD medium of potato dextrose broth, pH 5.1 (manufactured by DIFCO) at 2.9 × 10 ⁷ / ml. Was inoculated with 10 ml of the root rot bacterial wilt solution. The antagonistic bacterium AP-1 is composed of 10 g of meat extract, 10 g of peptone, 5 g of sodium chloride, 1,000 ml of tap water,
5.8 × 10 5 cultured in a meat extract liquid medium of pH 7.1
⁸ / ml was used. Pure tomatoes were used as pure tomatoes, and those having the true leaf at the 5th to 6th leaf stage were planted four times per plant / pot. Inoculation of the antagonistic bacterium AP-I
The tomato root at the time of planting was immersed in one culture solution. Forty-two days after planting, the stems at the ground were cut to examine the browning of the conduit and the root. Table 3 shows the results.

[0038]

[Table 3]

A against the root rot of tomato described above
As is clear from the results of the pot test for P-1, the antagonistic bacteria AP-1 section had a conduit browning degree of 16.7 and the control section had a browning degree of 50.
It was remarkably lower than 0, and the invasion of root rot fungi into tomato plants was suppressed.

[0040]

Example 4 Pot Test of Antagonistic Microbial Material against Tomato Root Rot Wilt To investigate the antagonistic effect of the antagonistic bacterium AP-1 microbial material, a low volume ratio of gray lowland soil and a commercially available horticultural cultivation soil as in Example 3 was used. After 500 g of the 1: 1 mixed soil was sterilized 3 times at 121 ° C. for 1 hour, the mixture was filled into a polypot having a diameter of 12 cm, and 10 ml of 4.2 × 10 ⁸ / ml root rot fungus was added to each pot.
Was inoculated. Antagonist bacteria AP-1 microbial material is meat extract 1
0 g, 2 g of yeast extract, 10 g of peptone, 2 g of sodium chloride, 1,000 ml of distilled water, and a bacterial solution of 10 ⁹ / ml or more of AP-1 cultured in NY medium having a pH of 7.0 were sprayed together with the culture solution on coffee compost. A mixture (AP-1 coffee compost material (liquid)), a mixture of coffee extract cake and a culture bacterial solution sprayed and mixed with coffee compost (AP-1 coffee compost material (cake)), and a coffee compost with AP-1 Mutant strain culture solution sprayed and mixed (AP-1S1 coffee compost material (liquid), AP-1R1 coffee compost material (liquid), AP
-1SR1 coffee compost material (liquid)). Pure tomatoes were used as pure tomatoes, and those having the true leaf at the 5th to 6th leaf stage were planted four times per plant / pot. Antagonistic bacteria AP-1
The inoculation of the microbial material is performed by adding the material 11.2 to 500 g of the test soil.
5 g were mixed. 31 days after planting, the stem at the ground was cut off, and the browning of the conduit and the root were examined. Table 4 shows the results.

[0041]

[Table 4]

As is clear from the results of the above pot test of the antagonistic microbial material against root rot of tomato,
The degree of conduit browning of the antagonistic bacterium AP-1 is 0.0-6.3 and AP
-1R1 1 of control plot except coffee compost material (liquid)
It was remarkably lower than 2.5, and the invasion of root rot fungi into tomato plants was suppressed.

[0043]

Example 5 Field Test of Tomato Root Rot Wilt Antagonistic Bacterium AP-1 Bacterium AP-1 was cultured in the same meat extract liquid medium as in Example 1 and 30 L of perlite 70 L (about 11.6)
kg), glycerin 8.125 L, tap water 8.125 L,
Using an AP-1 antagonistic bacterial material which was mixed with 14 g of magnesium sulfate (MgSO ₄ .7H ₂ O) and sterilized at 121 ° C. for 30 minutes and used as a material, the disease was developed in a field contaminated with tomato root rot. The deterrent effect and tomato yield were investigated. Tomato was planted in October 1995 and harvested from late January to early April using first power (sakata seeds) as a test variety. The test field area was 12.8 per ward.
m ² (26 plants planted). The root rot fungus was cultured in a PD medium for 2 weeks in the same manner as in Example 3, and 417 ml per 12.8 m ^{2 of a} section was mixed with 1.7 kg of rice straw and inoculated. Thereafter, the chlorpicrin agent was treated with 20 L per 10a, covered with a polyethylene film for 7 days to disinfect the soil, and chlorpicrin was degassed. This was used as a control group, and the AP-1 group was then used as an antagonistic bacterial AP-
1 material per 3,000 kg / 10a (38.4
kg / 12.8 m ² ) and mixed with soil up to a depth of 25 cm. N, P ₂ O ₅ , K ₂ O 10 kg / each
10a, N, P ₂ O ₅ , K ₂
O 10 kg / 10a each was applied twice. During the test, the yield was investigated. Table 5 shows the results.

[0044]

[Table 5]

As is clear from the results of the field test of the above-mentioned tomato root rot fungus antagonistic bacterium AP-1, the number of dead strains caused by root rot was 18 in the control group.
The antagonistic bacterium AP-1 group had 0 strains, and the difference in the number of dead strains appeared as a difference between the total yield and the yield per strain. The total yield of the control was 142.30 kg, while that of the antagonistic bacteria AP-1 group was 254.30 kg.
Comparing the control group to 100 with 89 kg, the antagonistic bacteria AP-1
The ward had a good result of 179. The yield per strain was 2.74 kg in the control group, while the antagonistic bacteria AP-
In the first ward, it was 4.90 kg, which was 2.16 kg. By the antagonistic bacterium AP-1, the occurrence of tomato root rot was suppressed until the tomato was harvested, and the yield of tomatoes tended to be excellent.

[0046]

According to the present invention, disease caused by tomato root rot fungus can be suppressed, tomato can be grown healthy, the yield can be increased, and labor saving can be achieved.

────────────────────────────────────────────────── ─── Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI // (C12N 1/20 C12R 1:40) (72) Inventor Mineo Sugiyama Shizukatsu-cho, Katsunosho-ji, Tatsukasha-cho, Nishi-Kasugai-gun, Aichi Prefecture 45 -2 Pokka Corporation Central Research Laboratories (72) Inventor Naoki Nakano Aichi Prefecture Nishi-Kasugai-gun Shikatsu-cho Oji Kumanosho 12-2 45-2 Pokka Corporation Central Research Laboratories (72) Inventor Masato Yamada Aichi Aichi Prefecture Gunaga Kute Town, Iwasaku, Mikamine 1-1 Aichi Prefectural Agricultural Research Center (56) References JP-A-3-220108 (JP, A) JP-A 8-245328 (JP, A) JP-A 7- 298874 (JP, A) Aichi Prefectural Agricultural Research Station Research Report, 1992, No. 24, p. 131-137 (58) Field surveyed (Int. Cl. ⁷ , DB name) C12N 1/00-7/08 BIOSIS (DIALOG) WPI (DIALOG)

Claims (7)

(57) [Claims] 1. A new microorganism Pseudomonas putida A that suppresses the onset of the soil-borne disease "tomato root rot of tomato" on tomato.
P-1 (FERM P-15828). 2. A mutant bacterium, Pseudomonas var., Which suppresses the onset of "tomato root rot and is resistant to the antibiotic streptomycin" obtained by mutating the microorganism of claim 1. Pseudomon
as putida) AP-1S1 (FERM P-1
6030) or Pseudomon, a mutant bacterium that suppresses the onset of “tomato root rot” and is resistant to the antibiotic rifampicin.
as putida) AP-1R1 (FERM P-1
6031) or a mutant Pseudomonas putida A that suppresses the development of "tomato root rot wilt" and exhibits resistance to the antibiotic streptomycin and the antibiotic rifampicin.
P-1SR1 (FERM P-16032). 3. Soil improvement by inoculating the microorganism according to claim 1 or the mutant bacterium according to claim 2 into an organic material such as compost or bran or coffee extract cake or an inorganic material such as perlite or vermiculite. A microbial material that inhibits the action of tomato root rot, a disease transmitted by tomato, which has an action. 4. An agent for controlling the onset of tomato soil-borne disease, "tomato root rot," comprising the microorganism according to claim 1 or the mutant bacterium according to claim 2 as an active ingredient. 5. Inhibition of tomato root-borne wilt of tomato, which is obtained by treating the soil with the microorganism material according to claim 3 or the disease-controlling agent according to claim 4. Method. 6. A soil-borne disease “tomato of tomato” comprising treating a soil or a nutrient solution in a culture for raising seedlings or a nutrient solution using the microorganism according to claim 1 or the mutant bacterium according to claim 2. A method to control the onset of root rot. 7. A soil-borne disease of tomato, comprising immersing roots or seeds of a plant in a suspension of cultured cells of the microorganism according to claim 1 or the mutant bacterium according to claim 2. A method for controlling the onset of root rot of tomato.