JP3991092B2 - Potato scab inhibitor and its use. - Google Patents

Description

The present invention relates to a technique for suppressing the occurrence of common scab, which has a large effect on potato harvest, using Bacillus thuringiensis A324 strain (accession number FERM P-19713).

  Potato common scab grows on the surface and inside of the potato, creating a brown lesion and causing brown lesions on the surface. Potato affected by this common scab will lose its commercial value and will greatly affect potato harvest.

  Conventionally, as a method for suppressing diseases caused by fungal infection or bacterial infection in plants and fruits, a method using a culture of a bacterium of Bacillus thuringiensis strain AQ52 (NRRL deposit number B21619) having antibacterial activity is disclosed in JP-T-2001-2001. No. 524806 is known.

This Bacillus thuringiensis strain, when combined with other bacterial strains, suppresses the blight of tomato plants and inhibits the growth of beetle larvae. However, it is not known to be effective for potato scab.
In addition, no bacteria have been known that effectively suppress the potato scab.
JP-T-2001-524806

  The problem to be solved by the present invention is to solve the conventional problems and provide a potato scab suppressor and a scab control method using bacteria that can effectively suppress potato scab. .

The configuration of the present invention that solves this problem is as follows.
1) Potato scab inhibitor 2 using Bacillus thuringiensis A324 strain (Acceptance number FERM P-19713) as an effective bacterium 2) Bacillus thuringiensis as a granular carrier ) Potato scab inhibitor according to 1) above, which contains A324 strain (acceptance number FERM P-19713) 3) Bacillus thuringiensis A324 strain (acceptance number FERM P- 19713) cultured in a liquid medium in a liquid form, the potato scab inhibitor according to 1) 4) Bacillus thuringiensis A324 strain (Acceptance number F) ERM P-19713) cultured in a solid medium in a solid form, the potato scab inhibitor according to 1) 5) The potato scab inhibitor of 1) to 4) is added to a fertilizer to reduce scab. Potato fertilizer that can be suppressed 6) The potato scab suppressant of 1) to 4) above can be added to the pesticide to suppress scab, 7) Pesticide for potato 7) Any of 1) to 4) above to the soil Potato cultivated soil inoculated with scab control agent to suppress the occurrence of scab disease of potato 8) Sprinkle of scab control agent of any one of 1) to 4) above or sprayed into the field suppress the generation of pressurized disease, scab suppression method 9 potato) said seed tuber of potato 1) to 4) one of the seed tuber surface in contact with the scab inhibitor Bacillus thuringiensis Jen cis (Bacillus thu ingiensis) A324 strain (with planting in the field from deposited acceptance number FERM P-19713), suppress the occurrence of potato scab, in scab disease suppression method of the potato.
As the granular carrier of the present invention, those made of crustacean powder such as calcium carbonate, zeolite, vermiculite, crab, shrimp, etc. are generally used. The product is produced by adding and mixing Bacillus thuringiensis A324 strain (accession number FERM P-19713) to this carrier.
In addition, as the solid medium of the present invention, a liquid medium containing a solidified medium by adding agar is used, but food waste such as rice bran, bran and shochu lees can be used effectively. These are added to the carrier to contain Bacillus thuringiensis A324 strain (accession number FERM P-19713).
In addition, the underline of the English description of the microbe in this specification shows that it is the italic notation of the microbe.

According to the present invention, potato scab can be effectively suppressed by using Bacillus thuringiensis A324 strain (accession number FERM P-19713) as an effective bacterium (culture thereof). . In addition, as an application method, scab disease is caused by spraying or swallowing potato scab suppressor of Bacillus thuringiensis A324 strain (accession number FERM P-19713) into soil or cultivated soil. Potato seed pods that can be made into fields and soils that are unlikely to occur, and contacted with the scab control agent of the present invention, have the effect that the occurrence of scabs can be extremely reduced.

The Bacillus thuringiensis A324 strain (accession number FERM P-19713) of the present invention is mixed and cultured with other bacteria (antibacterial microorganisms) that suppress harmful pathogens. It is preferable to apply to the soil. Alternatively, the scab control can be significantly suppressed by contacting the culture with the seed pod.
The most preferred method of using the potato scab control agent is to apply seed pods directly to the inhibitor, or to spray them directly onto the soil before sowing the seed pods.

The properties of Bacillus thuringiensis A324 strain (accession number FERM P-19713) used in the present invention will be described.
1. Physiological properties * Gram staining positive * facultative aerobic * motility positive * Neisseria gonorrhoeae (with linkage)
* Spore formation (elliptical diameter: spore location unevenly distributed)
* Make a circular parasital crystal next to the spore.

  The results of the biochemical property test are shown in Table 2.

Table 3 shows the characteristics of the Bacillus thuringiensis A324 strain (accession number FERM P-19713) used in the present invention.

Information on deposit of Bacillus thuringiensis A324 strain (accession number FERM P-19713) used in the present invention Depositary: National Institute of Advanced Industrial Science and Technology Special Biological Depository Accession number: FERM P-19713
Taxonomic position: Bacillus thuringiensis
Display for identification: A324

(Common scab control study)
Hereinafter, a confirmation test (field test) was conducted in order to confirm the effect of potato scab of the Bacillus thuringiensis A324 strain (accession number FERM P-19713) of the present invention. The test was conducted using 1 / 5000a and 1 / 2000a Wagner pots, and the test conditions are shown in Table 4 below and FIG.
From Table 6, no disease was observed in A324, and it was confirmed that the A324 strain has antibacterial activity against potato scab.

The test procedure will be described in detail below.
1. August 25 Streptomyces scabies ( Streptomyces scabies ) NBRC 13767 strain, which is the source of potato scab, in 1 L of compost / soil equivalent medium (below) in Maltose-Bennett's liquid medium (below) 10 ml of the culture solution obtained by shaking culture in a test tube at 28 ° C. and 121 min ^{−1 for} 7 days was added, stirred, and cultured in a 28 ° C. incubator.

2. August 30 Soil 15 kg was mixed with 1 L of sterilized napra, 2 L of vermiculite, 1 L of oil cake, 2 L of peat moss and 10 g of calcium carbonate, and sterilized with high-pressure steam to prepare soil for field tests.

3. September 3 Potato seed pods (variety: Dejima) were washed with tap water and dried indoors.

4. September 10 (1) In the 1 / 5000a Wagnener pot, Packed with 3 kg of sterilized soil prepared in
(2) Bacillus thuringiensis A324 strain was inoculated into one platinum ear in a 100 ml Erlenmeyer flask containing 8 ml of BT liquid medium (below), and shaking culture was started at 28 ° C. and 121 min ⁻¹ .
BT liquid medium meat extract 10g
Polypeptone 10g
NaCl 2g
1000ml distilled water
pH 7.6

5. September 11 (1) 792 ml of BT prepared from a 24-hour culture of Bacillus thuringiensis A324 strain precultured in 4- (2) in a 2000 ml stirred Erlenmeyer flask The whole amount was transferred to a liquid medium, and the start of main culture at 28 ° C. and 150 rpm was started on an 800 ml scale.
(2) In the 1 / 2000a Wagner pot, Packed with 7.5 kg of sterilized soil prepared in.
(3) 500 ml in the 1 / 5000a Wagnener pot prepared in 4- (1), 1L of 1000-fold diluted solution of “Kureai-binder soil organic urea complex liquid fertilizer 046” in the 1 / 2000a Wagnener pot prepared in 5- (2) Scattered.
(4) Streptomyces scabies NBRC13767 strain of pathogenic bacteria of 10 g (5 test sections) in 1 / 5000a wagnenel pot prepared in 5- (3) and 25 g (1 test section) in 1 / 2000a wagnenel pot A compost / soil equivalent medium cultured at 28 ° C. for 17 days was added to the rhizosphere and stirred.
(5) The same disease as 5- (4) was prepared as the diseased area of 5- (4).
(6) As a control group of 5- (4), a compost / soil equivalent medium in which the pathogen Streptomyces scabies NBRC13767 strain is not cultured in the Wagner pot prepared in 5- (3) 10 g (5 test sections) was put into a 1 / 5000a Wagnener pot, and 25 g (1 test section) was put into a 1 / 2000a Wagnener pot and stirred.

6. September 12 (1) After soaking the seed cake washed with 3 in 70% EtOH for 3 minutes, it was dried and sterilized.
(2) A 1 / 5000a Wagnener pot prepared in 5- (4), which was cultured for 24 hours in Bacillus thuringiensis A324 strain in 5- (1), was added to 100 ml of rhizosphere soil and stirred. did. (5 test zones)
(3) 6- (2) control zone and disease zone as 5- (5), 5- (6) 1 / 5000a Wagnener pot sterilized BT liquid medium only (sterile) 100ml each in the rhizosphere Added and stirred. (The control zone and disease zone of the 1 / 5000a Wagnener pot are 5 test zones each.)
(4) Into the 1 / 2000a Wagnener pot prepared in 5- (4), seed sterilized in 6- (1) was immersed in a 24-hour culture of Bacillus thuringiensis A324 strain for 30 minutes, and seed culm was added. (Seeding). (1 test zone).
(5) As a control group and a diseased group in 6- (4), in a 1 / 2000a Wagner pot prepared in 5- (5) and 5- (6), in a sterilized BT liquid medium only (sterile), 6- ( The seed sterilized in 1) was soaked for 30 minutes, and one seed pod was introduced (seeding). (Control zone and disease zone are each one test zone.)

7. September 13 1 / 5000a Wagnener pot prepared in 6- (2) and 6- (3) was charged with one seed sterilized in 6- (1) (seeding).

8. September 30 Picked out many potato sprouts and made three. (Buds)

9. Oct. 2 Oct. 24

10.12.26 Harvest of potatoes from each test area, lesion detection record

11. December 29 The potato harvested at 10 was washed with tap water, and lesions were determined and recorded again.

  As a result of the above, when the presence or absence and degree of scab of the harvested potato were determined, the results shown in Table 5 below were obtained.

As can be seen from Table 6, the Bacillus thuringiensis A324 strain of the present invention has no diseased pot, the potato is growing healthy, and the Bacillus thuringiensis A324 strain is It was found that the potato scab or fungus was suppressed. In other strains of Bacillus thuringiensis, lesions and rot were observed, which did not sufficiently suppress the common scab.

  In addition, watering during the cultivation period was carried out sparingly and adjusted to a state where the soil was slightly moist to dry.

  The potato scab control agent of the present invention is used as an antibacterial agent for other plant diseases by adding many other antibacterial BT bacteria and antibacterial microorganisms in addition to the control of potato scab. Can be used.

It is explanatory drawing which shows the use condition of the Wagnener pot of 0 ward, 1 ward, and 9 ward used for Example 1. FIG.

Explanation of symbols

1 1 / 5000a Wagner Pot 2 1 / 2000a Wagner Pot

Claims (9)

A potato scab suppressor comprising Bacillus thuringiensis A324 strain (accession number FERM P-19713) as an effective bacterium. The potato scab inhibitor according to claim 1, which is in the form of granules, wherein Bacillus thuringiensis A324 strain (accession number FERM P-19713) is contained in the powdery carrier. The potato scab inhibitor according to claim 1, which is a liquid obtained by culturing Bacillus thuringiensis A324 strain (accession number FERM P-19713) in a liquid medium. The potato scab inhibitor according to claim 1, which is a solid form obtained by culturing Bacillus thuringiensis A324 strain (accession number FERM P-19713) in a solid medium.   The potato fertilizer which can suppress a common scab by adding the potato scab inhibitor of Claims 1-4 to a fertilizer.   The potato pesticide which can suppress a common scab by adding the potato scab inhibitor of Claims 1-4 to a pesticide.   The potato cultivation soil which inoculates the scab of any one of Claims 1-4 to soil and suppresses the occurrence of scab of potato.   A method for suppressing scab disease of potatoes, wherein the scab disease inhibitor of any one of claims 1 to 4 is sprayed or applied to a field to suppress the occurrence of scab disease of potatoes. A potato seed pod is brought into contact with the scab control agent according to any one of claims 1 to 4 and Bacillus thuringiensis A324 strain (accession number FERM P-19713) is attached to the surface of the seed pod. Planting the potato scab to prevent potato scab development.

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