JP2018139533A - Plant disease control material using dicyma pulvinata - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a plant disease control material comprising novel microbes that has an excellent effect of controlling tomato leaf mold disease.SOLUTION: A novel Dicyma pulvinata strain 414-3 which is suitable as a plant disease control material excellent for controlling tomato leaf mold disease and a plant disease control method using same are provided.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a strain belonging to a novel Decima purbinata, a plant disease control material containing the strain, and a plant disease control method using the plant disease control material.

Pesticides sold for the purpose of plant disease control are regulated by the Agricultural Chemicals Control Law, and microorganisms having effective disease control ability are registered and sold as microbial pesticides.
Examples of microbial pesticides that have been registered and sold include bacteria such as non-pathogenic Erbinia carotobola, Bacillus subtilis, Pseudomonas fluorescens, and filamentous fungi such as Talalomyces flavus and Trichoderma atrobide. Is used for controlling diseases such as gray mold, powdery mildew, anthrax, soft rot, and rice seed infectious diseases, targeting vegetables, fruit trees and rice.

On the other hand, among vegetables, tomato is known to be a crop that is often damaged by pests. As diseases of tomato, plague, leaf mold, gray mold, powdery mildew, ring rot, etc. are known. In particular, leaf mold is one of the important diseases and has become a global problem. ing.
Tomato leaf mold occurs only on leaves. At first, pale yellow and unclear lesions are formed on the surface of the leaf, and a velvety flora of grayish yellow to greenish brown is densely grown on the back surface. As the disease progresses, the color of the flora increases from brown to grayish purple, the lesions expand and eventually die. When the disease is severe, most leaves are affected, causing many lesions and withering from the lower leaves, resulting in poor fruiting, insufficient fruit enlargement, and early coloring, resulting in a drastic decrease in yield. The pathogenic fungus is an airborne fungus that frequently occurs under humid conditions such as institutional cultivation, and is a disease that occurs nationwide in Japan.

Microbial pesticides are widely used in reduced pesticide cultivation due to advantages such as generally low environmental burden, no risk of appearance of resistant bacteria, and not counted in the number of components used. It is also ideal as an alternative control method for chemical pesticides that have developed resistant bacteria.
However, there are very few microbial pesticides registered for pesticides for tomato leaf mold, and only the Talaromyces flavus reported in Patent Documents 1 and 2, etc., except for the Bacillus agent. In addition, there is a problem that microbial pesticides still have no satisfactory performance in terms of preventive effects, therapeutic effects, and sustainability of disease control effects.

International Publication No. 2002/035934 Japanese Patent Laid-Open No. 2015-093850

As part of the countermeasure against tomato leaf mold, varieties having resistance genes against pathogenic bacteria are commercially available. However, 12 types of parasitically differentiated lines called races have emerged that overcome the resistance of these resistant varieties. With these diversified races, all resistant varieties marketed in Japan have been destroyed, and the emergence of resistant bacteria to chemical pesticides has been reported, and effective control methods with less environmental impact are required at production sites. Yes.
Therefore, the present invention provides a plant disease control material containing a novel microorganism having an excellent control effect against tomato leaf mold, and a plant disease control method using this plant disease control material. It is an issue.

  As a result of intensive studies to solve the above-mentioned problems, the present inventor has found a novel Dicyma pulvinata 414-3 strain as an excellent plant disease control material against tomato leaf mold disease. It has come to be solved.

The gist of the present invention is specifically as follows.
1. Dicyma pulvinata 414-3 strain (Accession number: NITE P-02413).
2. Plant disease control material containing Dicyma pulvinata 414-3 strain.
3. 1. Plant diseases are diseases caused by phytopathogenic fungi, The plant disease control material as described.
4). 1. Plant diseases are transmitted from leaves. Or 3. Plant disease control material as described in 1.
5. 1. the plant disease is tomato leaf mold ~ 4. The plant disease control material in any one.
6.2. ~ 5. A plant disease control method using the plant disease control material according to any one of the above.
7.2. ~ 5. 5. A plant disease control material according to any one of the above is applied to a plant. A method for controlling plant diseases as described in 1. above.

The Dicyma pulvinata 414-3 strain of the present invention has been confirmed to exhibit fungal infestation parasitic on tomato leaf mold, and thus has excellent disease control activity against tomato leaf mold. In addition, it was confirmed in the pot test in the room and the field test in the greenhouse that a high disease control effect against tomato leaf mold was exhibited.
In addition, the Dicyma pulvinata 414-3 strain is parasitic on all the diverse races of tomato leaf mold that occur in Japan, so it has an effective control effect on any race. Can be obtained.
The Dicyma pulvinata 414-3 strain of the present invention exhibits high fungal parasitism under high humidity conditions, so that it can be used effectively in cultivation sites where tomato leaf mold disease is likely to occur, and has become ill. It can be applied to tomatoes and can also prevent secondary infections.
The Dicyma pulvinata 414-3 strain has a high conidia (spore) forming ability and can be obtained at a low concentration that exhibits an effective control effect against tomato leaf mold. There is merit in manufacturing as.

It is a figure which shows the molecular phylogenetic tree based on the base sequence of an ITS area | region. It is the nucleotide sequence of the ITS region of Dicyma pulvinata 414-3 strain. It is an electron micrograph which shows the parasitic nature of Dicyma pulvinata (Dicyma pulvinata) 414-3 stock | strain by Example 2, the method 1. FIG. It is the optical micrograph and fluorescence micrograph which show the parasitic nature of the Dicyma pulvinata 414-3 stock | strain by Example 2, the method 2. FIG. It is a graph showing the spore formation capability confirmation test result of the Dicyma pulvinata 414-3 strain | stump | stock of Example 3. “ITS” is an abbreviation of “Internal transcribed spacer”, and “ITS base sequence” refers to the base sequence used for molecular phylogenetic analysis in filamentous fungi, like the 16s rDNA sequence in bacteria. means.

The present invention relates to a strain belonging to Dicyma pulvinata, which has an excellent disease control effect against tomato leaf mold.
The present invention also relates to a plant disease control material containing the strain and a plant disease control method using the plant disease control material.
Hereinafter, the present invention will be described in detail.
(New strain)
The novel strain belonging to Dicyma pulvinata in the present invention has an excellent disease control effect against tomato leaf mold.
This Dicyma pulvinata 414-3 strain has been deposited with the Patent Microorganism Depositary Center for Product Evaluation Technology, an independent administrative agency, and the deposit number is NITE P-02413.

Examples of the bacteriological properties of Dicyma pulvinata 414-3 include "property suitable for controlling plant diseases". Specifically, it has excellent disease control activity against tomato leaf mold. It is what has. This excellent disease control activity is exhibited by the high fungal parasitic property against tomato leaf mold. In particular, it has been confirmed by the test described later that it is parasitic on all the various races of tomato leaf mold occurring in Japan.
In the present invention, the Dicyma pulvinata 414-3 strain is a tomato in a cultivation facility where tomato leaf mold disease has occurred, specifically, in a cultivation facility in which the diffusion suppression phenomenon of tomato leaf mold disease is observed. Filamentous fungi that cover the fungal flora of leaf mold are applied to elementary agar medium, and single conidia (spores) are separated and cultured multiple times, then isolated as a strain, and are capable of controlling disease and morphological properties. They were selected by examining molecular phylogenetic characteristics. Further, Dicyma pulvinata can be rarely isolated from the naturally occurring leaf of tomato leaf mold as a fungal parasitic fungus against tomato leaf mold.
A person skilled in the art can select a strain having a high parasitic ability against tomato leaf mold and exhibiting a high control effect against tomato leaf mold by, for example, pot test, among the isolated strains of Dicyma pulvinata. This can be done easily.
The Dicyma pulvinata 414-3 strain of the present invention is a fungal parasite that has a high tomato leaf mold control effect, but does not show pathogenicity to plants.

The culture method of the Dicyma pulvinata 414-3 strain is not particularly limited, and can be cultured by a method generally applied to microorganisms belonging to the Decima purvinata.
As the medium, for example, a general medium for filamentous fungi such as potato squeeze agar medium (PSA medium) with sucrose, potato scent agar medium (PDA medium) with glucose, or oatmeal agar medium can be used. The culture temperature is preferably 20 to 25 ° C.

(Plant disease control materials)
The plant disease control material of the present invention contains the Dicyma pulvinata 414-3 strain, and the plant disease control material will be described.
As the plant disease control material, Dicyma pulvinata 414-3 strain can be formulated and used. Formulation forms include lyophilized Dicyma pulvinata 414-3 strain in powder form, solidified with excipients, filled into capsules, liquid ampules Further, it can be in the form of a pharmaceutical preparation similar to that of a normal microbial pesticide, for example, a wettable powder, a flowable agent, an emulsion, a powder, a granule, a soil mixture or the like. Moreover, you may use the formulation adjuvant for formulation like a normal microbial pesticide. Examples of such formulation aids include carriers, adjuvants, surfactants, and dispersants.

  As the carrier, inorganic or organic materials that are usually used for microbial pesticides can be used as the main material. Specifically, as the solid carrier, red jade earth, calcined red jade earth, Kanuma earth, black meso earth , Acid clay, talc, clay, kaolin clay, pyrophyllite clay, bentonite, montmorillonite, diatomaceous earth, synthetic hydrous silicon oxide, vermiculite, perlite, zeolite, attapulgite, Otani stone, anthracite, coal ash, lime, salt, carbonate Inorganic materials such as salt, sulfate, nitrate, urea, rice husk, bran, wheat flour, corn cobs, peanut husk, peat moss, pulp, straw, bagasse, wood flour, rice bran, rice bran powder, oil cake, starch, fish cake, Bone meal, dried animal dung, crab, shrimp, krill fine powder, charcoal, kun charcoal, bark charcoal, rice husk kun charcoal, grass charcoal, active , Can be used ash, fossil shells, sorbitol, lactose, maltitose, glucosamine, the organic materials such as oligosaccharides. As the liquid carrier, water, vegetable oil, animal oil, mineral oil, synthetic water-soluble polymer and the like can be used. These components can be used alone or as a mixture of two or more.

Furthermore, the plant disease control material of the present invention includes, as an adjuvant, natural polysaccharides such as casein, gelatin, gum arabic, alginic acid, celluloses, carboxymethylcellulose, xanthan gum, chitins, chitosans, polyvinyl alcohols, polyacrylic acids, etc. Bentonite and the like can be added as necessary for the purpose of thickening, fixing, dispersing and the like. In addition, dihydric alcohols such as ethylene glycol and propylene glycol can be included as necessary for the purpose of preventing freezing. Furthermore, anionic, cationic and amphoteric surfactants can be included as needed for the purpose of dispersion stability, aggregation prevention, emulsification and the like.
The plant disease control material of the present invention is mixed with at least one of fungicides, insecticides, nematicides, acaricides, herbicides, plant growth regulators, fertilizers, soil improvement materials and the like when applied. Alternate application or simultaneous application without application or mixing is also possible.

The concentration of Dicyma pulvinata 414-3 strain in the plant disease control material of the present invention is not particularly limited, but the conidia (spore) concentration is 0.01 to 10 × 10 ⁷ cells / ml. Preferably, it is more preferably 0.5 to 1.0 × 10 ⁷ pieces / ml.
The application amount of the plant disease control material of the present invention is not particularly limited as long as it is within the range in which plant diseases can be controlled, but usually 10 ⁵ to 10 ¹⁰ cfu, preferably 10 ⁶ to 10 ⁸ per 1 m ^{2 of} the field. Spread cfu.

The plant disease in the present invention is not particularly limited as long as the Dicyma pulvinata 414-3 strain of the present invention exhibits a control effect. However, the plant disease is caused by infecting a plant with a pathogenic fungus. Disease is preferred. Among them, plant diseases caused by pathogenic bacteria infecting the above-ground parts of plants such as stems, leaves, flowers, fruits and the like are preferable, and in particular, plant diseases caused by infecting plant leaves with pathogenic bacteria are preferable. More preferred.
Such plant diseases include, specifically, tomato leaf mold caused by Cladosporium fulvum, tomato, eggplant, pepper, strawberry, cucumber, lettuce, caused by Botrytis cinerea. Gray mold such as green beans, cabbage black soot caused by Alternaria brassicicola, tomato plague caused by Phytophthora infestans, peanut black caused by Cercosporidium personatum Astringency, tomato wilt caused by Fusarium oxysporum, cucumber, melon, etc. caused by Sphaerotheca fuliginea Powdery mildew, red pepper caused by Leveillula taurica, powdery mildew such as pepper, tomato, powdery mildew such as eggplant caused by Erysiphe cichoracearum, Pseudoperonospora cubensis Cucumber downy mildew caused by Pythium sp., Cucumber rot caused by Puccinia allii, leek rust caused by Puccinia allii, leeks micronuclei caused by Sclerotinia allii Disease, cabbage black spot caused by Alternaria brassicae, carrot blight caused by Alternaria dauci, caused by Rhizoctonia solani Spinach strain rot, Sclerotinia sclerotiorum caused by Sclerotinia sclerotiorum, lettuce nuclear disease, Verticillium dahliae caused eggplant wilt, Sphaerotheca humuli caused by strawberry noodles Diseases of vegetables such as strawberry anthrax caused by Glomerella cingulata, pea brown spot caused by Ascochyta pisi, and peanut red spot caused by Botrytis fabae Is mentioned. In addition, flower diseases include gray mold diseases such as cyclamen, chrysanthemum, roses, and staticis caused by Botrytis cinerea, rose powdery mildew caused by Sphaerotheca pannosa, and Puccinia horiana (Puccinia). for example, chrysanthemum rust caused by horiana). In addition, the plant diseases of fruit trees include white blight of fruit trees caused by Rosellinia necatrix, blue mold disease of citrus caused by Penicillium italicum, Diaporthe citri Citrus sunspot caused by scab, pear scab caused by Gymnosporangium asiaticum, apple scab caused by Venturia inaequalis, peach ash caused by Monilinia fructicola Etc. The diseases of the grass family include rice blight caused by Rhizoctonia solani, rice blast caused by Pyricularia oryzae, and barley udon caused by Erysiphe graminis. And wheat wilt caused by the disease, Gaeumannomyces graminis. Among these plant diseases, leaf mold disease is preferably mentioned as a plant disease in the present invention.

  The plant disease control material of the present invention includes tomato leaf mold, tomato, eggplant, bell pepper, strawberry, cucumber, lettuce, green beans, etc., gray mold, cabbage black soot, tomato plague, groundnut black astringency, tomato wilt Cucumber, melon, tomato, eggplant, pepper, pepper powdery mildew, cucumber downy mildew, cucumber blight, leek rust, leek sclerotia, Chinese cabbage black spot, carrot black leaf blight, spinach strain Diseases of vegetables such as rot, lettuce nuclear disease, eggplant half wilt, strawberry powdery mildew, strawberry anthracnose disease, pea brown spot disease, and broad bean red spot disease, cyclamen, chrysanthemum, rose, statice, etc. Plant diseases of flowering plants such as gray mold disease, rose powdery mildew, chrysanthemum rust, fruit tree white spotted feather disease, citrus blue mold disease, citrus black spot disease, pear red star disease, phosphorus It can be used to control plant diseases such as plant diseases of fruit trees such as black star disease and peach ash star disease, rice blight disease, rice blast disease, barley powdery mildew, and wheat disease .

  The plant disease control material of the present invention is applied to cultivated plants for the purpose of controlling various diseases of various cultivated plants as described above, and the method is appropriately selected depending on the use form such as dosage form, crops and diseases. For example, ground liquid spraying, ground solid spraying, airborne liquid spraying, airborne solid spraying, liquid surface spraying, in-facility application, soil mixing application, soil irrigation application, surface treatment (seed dressing, application treatment, etc.) Examples of the method include application, single flower treatment, and stock treatment. Moreover, in these application methods, the application to the above-ground disease which processes to the foliage of a cultivated plant is preferable.

  EXAMPLES Hereinafter, although an Example is given and this invention is demonstrated, the technical scope of this invention is not limited by these.

<Example 1> Isolation and identification of Dicyma pulvinata 414-3 strain (1) Isolation of strain White hyphae (parasitic strain) parasitic on lesions from leaves affected by leaf mold of tomato (Solanum lycopersicum) ) Was separated on an elementary agar medium (2%) and cultured at room temperature for 1 day. The germinated monospores on the medium were separated into PDA medium (potato dextrose agar) and cultured at 25 ° C. under a stereomicroscope. The same operation was further performed twice to obtain 414-3 strain. Cultured aseptically in PDA medium for 1 week, and conidia (spores) were recovered by rinsing the PDA plate with sterile water.

(2) Properties of strains The mycological properties of the 414-3 strain isolated from the above culture were examined.
The conidia pattern and conidia (spore) morphology of strain 414-3 were observed using an optical microscope, and the previously reported Dicyma pulvinata (Deighton, 1972; Hughes, 1951; Von Arx , 1981). In the homology analysis of the base sequence of the ITS region, the Dicyma pulvinata strains “222.63” and “254.59” of the Netherlands CBS-KNAW Fungal Biodiversity Center and KACC (Korean Agricultural Culture Collection) Using “KACC44498” and “KACC44502” and the base sequences of HMQAU130012, LCC11, and FMR12706 for comparison, it was revealed that they show high homology.
FIG. 1 shows a phylogenetic tree based on the base sequence of the ITS region, and FIG. 2 and the sequence table show the base sequence of the ITS region of the 414-3 strain of the present invention.

<Example 2> Confirmation of parasitic nature against leaf mold of Dicyma pulvinata 414-3
The parasitic nature of Dicyma pulvinata strain 414-3 was confirmed using two methods.
<Method 1>
Using a scanning electron microscope, it was confirmed that the 414-3 strain wrapped around the mycelium of the mold of fungi on tomato leaves and was parasitic (see the arrow in FIG. 3).
<Method 2>
A fungal fungus that constantly expresses the green fluorescent protein (GFP) gene was created, and an experimental system was constructed to determine the viability of the fungus using the fluorescence intensity. The GFP gene was ligated downstream of the promoter region of the glyceraldehyde-3-phosphatase dehydrogenase gene that is constitutively expressed in filamentous fungi (Aspergillus nidulans), and introduced into leaf mold by transformation. As long as they are alive, GFP-3 and 414-3 strains that constantly produce GFP are added to a minimal medium that does not contain a carbon source as a nutrient source (10 g KNO ₃ , 5 g KH ₂ PO ₄ , 2.5 g MgSO ₄ .7H ₂ O, 0.02 g FeCl ₃ , per liter).

As shown in FIG. 4, the result of confirming the parasitic property by Example 2 and Method 2 is that the GFP fluorescence is partially weakened in the mycelium of the leaf mold fungus contacted with the 414-3 strain on the third day of the culture. On the 4th day, the GFP fluorescence completely disappeared, so it was confirmed that the 414-3 strain was parasitic on the leaf mold and obtained a nutrient source. In FIG. 4, the left side is an optical micrograph and the right side is a fluorescence micrograph.
From the results of Methods 1 and 2, it was confirmed that the strain 414-3 showed fungal parasitic on the fungal fungus.

<Example 3> Confirmation of spore-forming ability of Dicyma pulvinata 414-3 strain On PDA medium, Dicyma pulvinata 414-3 strain, CBS222.63 strain, CBS254.59 strain, KACC44498 The strain, KACC44502 strain, was aseptically cultured for 1 week, and conidia (spores) were collected by rinsing the PDA plate with sterilized water. The test results are shown in FIG.
In FIG. 5, “222.63” is “CBS222.63 stock”, “254.59” is “CBS254.59 stock”, “44498” is “KACC44498 stock”, “44502” is “KACC44502 stock”, “414-3” means “414-3 strain” of the present invention.

As shown in FIG. 5, the 414-3 strain has a much larger amount of spore formation per colony formed on the medium than the CBS222.63 strain, the CBS254.59 strain, the KACC44498 strain, and the KACC44502 strain. It became clear.
Since microbial pesticides grow microorganisms by artificial culture and are used as a preparation, a strain having a higher ability to form spores by a general culture method can be formulated and industrialized at a lower cost. From this, it is thought that 414-3 stock | strain of this invention is advantageous in the manufacture surface at the time of setting it as a microbial pesticide.

<Example 4> Pot test against tomato leaf mold of Dicyma pulvinata 414-3 strain The inoculation test plant uses a tomato variety "Momotaro" that does not have a tomato leaf mold resistance gene, Transplanted 3-week-old tomato seedlings were used. Tomato leaf mold fungus (Cladosporium fulvum) strains “CF229” and “CF338” were cultured at 25 ° C. for 2 weeks using a PDA medium, and conidia (spores) were collected with distilled water and used.
About 5 ml of a spore suspension (10 ⁴ conidia / ml) of tomato leaf mold (Cladosporium fulvum) was sprayed on the back of the test plant to infect tomato leaf mold. After growing for 24 hours in a 100% humidity chamber, the chamber was moved to an artificial weather chamber (25 ° C., 16 hours light period-8 hours dark period). After confirming symptoms of leaf blight after about 2 weeks, the spore suspension of the separation parasitic strains to tomato leaf mold fungus shown below, spray inoculated into tomato (10 ⁵ cells / plants), and further 12 days after the tomato leaves The colony formation in the fungal lesion was visually confirmed.
The parasitic isolates for tomato leaf mold used were the 414-3 strain of the present invention, the CBS222.63 strain, and the CBS254.59 strain, and the 414-3 strain of the present invention was 3 times, the CBS222.63 strain and the CBS254 strain. The .59 strain was tested twice.
The test results were evaluated based on the control effect (%) and parasitic index shown in Table 1.
The control effect represents the ratio (%) of the number of diseased leaves with lesions parasitic on the isolated parasitic strain to the total number of diseased leaves in the test plant.
The parasitism index represents the average value of all the diseased leaves with lesions parasitic on the isolated parasitic strain, evaluated by the following criteria for the covering state of the lesion by the isolated parasitic strain.
Parasitic index 0: No covering of leaf mold spots by parasitic fungi Parasitic index 1: A state of covering 1/4 of lesions Parasitic index 2: Covering 1/4 to 1/2 of lesions State Parasitic index 3: A state where 1/2 to 3/4 of a lesion is covered Parasitic index 4: A state where 3/4 or more of a lesion is covered

  As shown in Table 1, the 414-3 strain of the present invention has an extremely high control effect against tomato leaf mold disease compared to the isolated parasitic strains “CBS222.63 strain” and “CBS254.59 strain” against the same tomato leaf mold. It became clear to play. This is considered to be derived from the high fungal parasitic ability of the 414-3 strain of the present invention against tomato leaf mold.

<Example 5> Field test of Dicyma pulvinata 414-3 strain against tomato leaf mold Inoculation test plant uses tomato variety "Momotaro" which does not have tomato leaf mold resistance gene for 3 weeks Age-old tomato seedlings were transplanted into a greenhouse, and tomato plants that had passed 2 months were used. The tomato leaf mold fungus (Cladosporium fulvum) CF229 strain was cultured at 25 ° C. for 2 weeks using a PDA medium, and conidia (spores) were collected with distilled water and used.
The spore suspension (10 ⁴ conidia / ml) of tomato leaf mold (Cladosporium fulvum) was sprayed on the back of the test plant to infect tomato leaf mold. After confirming the symptom of leaf mold after about 2 weeks, a spore suspension of the isolated parasitic strain 414-3 (Example) and distilled water containing no spores of the isolated parasitic strain 414-3 (Comparative Example) The lesions of tomato leaf mold were spray-inoculated (4 × 10 ⁴ conidia / leaf), and after 12 days, colony formation in the symptoms of tomato leaf mold was visually confirmed. Each test was performed twice.
The evaluation of the test results was performed based on the control effect (%) and the parasitic index shown in Table 2.
This control effect and parasitic index are the same evaluation contents as in Example 4.

As shown in Table 2, the 414-3 strain of the present invention showed a high control effect against tomato leaf mold even in the greenhouse.

<Example 6> Confirmation of controlling effect of Dicyma pulvinata 414-3 strain on tomato leaf mold race As described above, it is clear that tomato leaf mold fungus has 12 types of race in Japan. It has become. Therefore, the control effect of Dicyma pulvinata 414-3 strain on various races was confirmed.
As the inoculation test plant, a tomato variety “Momotaro” having no tomato leaf mold resistance gene was used, and a 3-week-old tomato seedling transplanted in a pot was used. Tomato leaf mold (Cladosporium fulvum) strain was cultured at 25 ° C. for 2 weeks using a PDA medium, and conidia (spores) were collected with distilled water and used.
About 5 ml of a spore suspension (10 ⁴ conidia / ml) of tomato leaf mold (Cladosporium fulvum) was sprayed on the back of the test plant to infect tomato leaf mold. After growing for 24 hours in a 100% humidity chamber, the chamber was moved to an artificial weather chamber (25 ° C., 16 hours light period-8 hours dark period). About 2 weeks later, after confirming the symptom of leaf mold disease, the spore suspension of 414-3 strain was spray-inoculated on the lesion of tomato leaf mold disease (10 ⁵ plants / plant). Colony formation in the disease symptoms was confirmed visually.
Evaluation of the test results was carried out by the control activity shown in Table 3.
This control activity was evaluated as “+” when the parasite was found to be 50% or more against the leaf spot of tomato leaf mold.

As shown in Table 3, the 414-3 strain of the present invention showed high control activity similarly to any race of tomato leaf mold.

The Dicyma pulvinata 414-3 strain of the present invention exhibits fungal parasitism parasitic on tomato leaf mold and is high against tomato leaf mold in both indoor pot tests and greenhouse field tests. It exhibits a disease control effect, and also exhibits high control activity against all the diverse races of tomato leaf mold that occur in Japan, and is expected to be used in cultivation situations.
Furthermore, since the 414-3 strain of the present invention has a high conidia (spore) forming ability, it is advantageous when it is produced as a microbial pesticide.

NITE P-02413

Claims (7)

  Dicyma pulvinata 414-3 strain (Accession number: NITE P-02413).   Plant disease control material containing Dicyma pulvinata 414-3 strain.   The plant disease control material according to claim 2, wherein the plant disease is a disease caused by a phytopathogenic fungus.   The plant disease control material according to claim 2 or 3, wherein the plant disease is infected from a leaf.   The plant disease control material according to any one of claims 2 to 4, wherein the plant disease is tomato leaf mold.   The plant disease control method which uses the plant disease control material in any one of Claims 2-5.   The plant disease control method according to claim 6, wherein the plant disease control material according to claim 2 is applied to a plant.