The composition that contains bacillus subtilis or Bacilluscereus, and Prochloraz
Technical field
The invention belongs to pesticide field, specifically originally relate to a kind of composition that contains bacillus and Prochloraz.
Background technology
Bacillus subtilis belongs to biological bactericide, and bacterial classification obtains brevibacterium from soil or axis separation.Be exclusively used in the Cotton seeds rice paddy seed, have the effect that activates plant growth, alleviates germ harm such as bacterial leaf streak of rice, bacterial leaf-blight, evil merit disease.The mode of occupying living space by competitive growth and breeding stops the growth of plant pathogenic fungi, can form the highly dense diaphragm of one deck rapidly in crop surface, makes phytopathogen can not get living space, thereby has protected farming with avoiding pathogen harm.Bacillus subtilis can secrete antibacterial substance, resists germ spore-germination and mycelial growth, thereby reaches prevention and therapeutic purposes.
Waxy Bacillus (bacillus cereus) belongs to the biogenic bactericide, and significant feature mechanism is to produce a large amount of antagonist proteins rice sheath blight disease is had preventive effect preferably.Simultaneously various vegetables soil-borne diseases such as cucumber fusarium axysporum also there is stronger antagonistic ability.
Prochloraz is a kind of wide-spectrum bactericide, and the disease that various crop is caused by sac fungi and imperfect fungus has tangible preventive effect, also can use with most of bactericide, bactericide, Insecticides (tech) ﹠ Herbicides (tech), and the better prevention effect is all arranged.Multiple diseases on field crop, fruit and vegetable, turf and the ornamental plants had treatment and eradicant action.
Summary of the invention
What the purpose of this invention is to provide that a kind of disease to multiple kinds of crops all has preventive and therapeutic effect is the composition of main active substances with bacillus and Prochloraz, alleviated the pesticide resistance of existing germ to Prochloraz, result of study shows that the combination of bacillus subtilis or waxy Bacillus and Prochloraz has the obvious synergistic effect.
Purpose of the present invention can reach by following measure:
A kind of composition that contains bacillus and Prochloraz, said composition is an active component with bacillus and Prochloraz, its weight ratio is 10000: 1~1: 10000; Wherein said bacillus is bacillus subtilis or Bacilluscereus.
The weight ratio of bacillus and Prochloraz is preferably 1000: 1~and 1: 1000, more preferably 100: 1~1: 100, more preferably 30: 1~1: 100, more preferably 30: 1~1: 30 again, further be preferably 10: 1~1: 30, most preferably 10: 1~1: 10.The spore count of living in the bacillus of the present invention (being bacillus subtilis or Bacilluscereus) is 0.1 hundred million/gram~1,000 hundred million/gram, is preferably 0.5 hundred million/gram~500 hundred million/gram, more preferably 100,000,000/gram~100 hundred million/gram.
In active component bacillus and Prochloraz, add can join after the insecticides adjuvant wetting powder, aqueous suspension agent or aqueous emulsion.After in active component bacillus and Prochloraz, adding auxiliary agent and regulating pH value 5~7, be made into wetting powder.Prochloraz can adopt former medicine, and bacillus subtilis or Bacilluscereus, can adopt former powder.Insecticides adjuvant can be selected in wetting agent, dispersant, filler, binding agent, the disintegrant etc. one or more for use.
Composition of the present invention has alleviated existing germ respectively to the pesticide resistance of Prochloraz and bacillus, after bacillus subtilis or waxy Bacillus and Prochloraz combination, has the obvious synergistic effect between the two, reduced simultaneously dosage again, thereby make said composition can be widely used in preventing and treating banded sclerotial blight, bakanae disease, rice blast or the false smut etc. of paddy rice, the downy mildew of tomato, the powdery mildew of cucumber, the powdery mildew of strawberry, gray mold, the crown rot of banana, anthracnose, the blossom-end rot of oranges and tangerines, anthracnose, penicilliosis, green mould etc.
Embodiment
Example of formulations 1:31% wetting powder (each component is a weight portion)
Bacillus subtilis (5,000,000,000 spore alive/gram) 30
Prochloraz 1
Lauryl sodium sulfate 5
Penetrating agent JFC 4
White carbon complements to 100 (adjust pHs 5~7).
Example of formulations 2:20% wetting powder (each component is a weight portion)
Bacilluscereus, (24,000,000,000 spore alive/gram) 10
Prochloraz 10
Emulsifier op-10 6
Dispersing agent NNO 4
Lactose 10
Starch complements to 100 (adjust pHs 5~7).
Example of formulations 3:60% wetting powder (each component is a weight portion)
Bacillus subtilis (20,000,000,000 spore alive/gram) 50
Prochloraz 10
Detergent LS 2
Diffusion agent N 4
Lauryl sodium sulfate 2
Precipitated calcium carbonate 32 (adjust pH 5~7).
Example of formulations 4:80% wetting powder (each component is a weight portion)
Bacilluscereus, (1,000 hundred million spore alive/gram) 20
Prochloraz 60
Draw back powder 2
Bay alcohol radical sodium sulphate 3
Lignin 4
White carbon 11 (adjust pH 5~7).
Drug effect embodiment 1
Bacillus subtilis (24,000,000,000 live in spore/gram) and Prochloraz are made into 20% wetting powder in the ratio of 1: 1 (mass ratio), spraying, be applied to treat bakanae disease of rice, separately bacillus subtilis and Prochloraz be made into above-mentioned wetting powder and contrast, the results are shown in Table 1.
Table 1
Drug effect embodiment 2
Bacillus subtilis (5,000,000,000 live in spore/gram) and Prochloraz are made into 31% wetting powder in the ratio of 30: 1 (mass ratio), spraying, be applied to treat tomato downy mildew, separately bacillus subtilis and Prochloraz be made into above-mentioned wetting powder and contrast, the results are shown in Table 2.
Table 2
Drug effect embodiment 3
Bacillus subtilis (50,000,000,000 live in spore/gram) and Prochloraz are made into 70% wetting powder in the ratio of 1: 30 (mass ratio), spraying, be applied to treat powdery mildew of cucumber, separately bacillus subtilis and Prochloraz be made into above-mentioned wetting powder and contrast, the results are shown in Table 3.
Table 3
Drug effect embodiment 4
Bacillus subtilis (20,000,000,000 live in spore/gram) and Prochloraz are made into 44% wetting powder in the ratio of 10: 1 (mass ratio), spraying, be applied to treat the banana crown rot, separately bacillus subtilis and Prochloraz be made into above-mentioned wetting powder and contrast, the results are shown in Table 4.
Table 4
Drug effect embodiment 5
Bacillus subtilis (1,000 hundred million live in spore/gram) and Prochloraz are made into 20% wetting powder in the ratio of 1: 100 (mass ratio), spraying, be applied to treat the oranges and tangerines blossom-end rot, separately bacillus subtilis and Prochloraz be made into above-mentioned wetting powder and contrast, the results are shown in Table 3.
Table 3