CN101574090A - Composition containing bacillus subtilis or waxy bacillus and prochloraz - Google Patents

Composition containing bacillus subtilis or waxy bacillus and prochloraz Download PDF

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Publication number
CN101574090A
CN101574090A CNA200910033299XA CN200910033299A CN101574090A CN 101574090 A CN101574090 A CN 101574090A CN A200910033299X A CNA200910033299X A CN A200910033299XA CN 200910033299 A CN200910033299 A CN 200910033299A CN 101574090 A CN101574090 A CN 101574090A
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bacillus
prochloraz
gram
bacillus subtilis
composition
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CNA200910033299XA
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CN101574090B (en
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刘东卫
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Jiangsu Futian Agrochemical Co Ltd
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Abstract

The invention discloses a composition containing bacillus subtilis or waxy bacillus and prochloraz, which uses bacillus and the prochloraz as active components according to a weight ratio of between 10,000:1 and 1:10,000, wherein the bacillus is the bacillus subtilis or the waxy bacillus. The composition reduces the drug resistance of the prior disease germs to the prochloraz and the bacillus respectively, and after combination, the bacillus subtilis or the waxy bacillus and the prochloraz have obvious synergistic action and the dosage is reduced at the same time, thus the composition can be widely applied to controlling sheath blight, bakanae disease, rice blast or false smut and the like of rice, downy mildew of tomato, powdery mildew of cucumber, crown rot of banana and the like.

Description

The composition that contains bacillus subtilis or Bacilluscereus, and Prochloraz
Technical field
The invention belongs to pesticide field, specifically originally relate to a kind of composition that contains bacillus and Prochloraz.
Background technology
Bacillus subtilis belongs to biological bactericide, and bacterial classification obtains brevibacterium from soil or axis separation.Be exclusively used in the Cotton seeds rice paddy seed, have the effect that activates plant growth, alleviates germ harm such as bacterial leaf streak of rice, bacterial leaf-blight, evil merit disease.The mode of occupying living space by competitive growth and breeding stops the growth of plant pathogenic fungi, can form the highly dense diaphragm of one deck rapidly in crop surface, makes phytopathogen can not get living space, thereby has protected farming with avoiding pathogen harm.Bacillus subtilis can secrete antibacterial substance, resists germ spore-germination and mycelial growth, thereby reaches prevention and therapeutic purposes.
Waxy Bacillus (bacillus cereus) belongs to the biogenic bactericide, and significant feature mechanism is to produce a large amount of antagonist proteins rice sheath blight disease is had preventive effect preferably.Simultaneously various vegetables soil-borne diseases such as cucumber fusarium axysporum also there is stronger antagonistic ability.
Prochloraz is a kind of wide-spectrum bactericide, and the disease that various crop is caused by sac fungi and imperfect fungus has tangible preventive effect, also can use with most of bactericide, bactericide, Insecticides (tech) ﹠ Herbicides (tech), and the better prevention effect is all arranged.Multiple diseases on field crop, fruit and vegetable, turf and the ornamental plants had treatment and eradicant action.
Summary of the invention
What the purpose of this invention is to provide that a kind of disease to multiple kinds of crops all has preventive and therapeutic effect is the composition of main active substances with bacillus and Prochloraz, alleviated the pesticide resistance of existing germ to Prochloraz, result of study shows that the combination of bacillus subtilis or waxy Bacillus and Prochloraz has the obvious synergistic effect.
Purpose of the present invention can reach by following measure:
A kind of composition that contains bacillus and Prochloraz, said composition is an active component with bacillus and Prochloraz, its weight ratio is 10000: 1~1: 10000; Wherein said bacillus is bacillus subtilis or Bacilluscereus.
The weight ratio of bacillus and Prochloraz is preferably 1000: 1~and 1: 1000, more preferably 100: 1~1: 100, more preferably 30: 1~1: 100, more preferably 30: 1~1: 30 again, further be preferably 10: 1~1: 30, most preferably 10: 1~1: 10.The spore count of living in the bacillus of the present invention (being bacillus subtilis or Bacilluscereus) is 0.1 hundred million/gram~1,000 hundred million/gram, is preferably 0.5 hundred million/gram~500 hundred million/gram, more preferably 100,000,000/gram~100 hundred million/gram.
In active component bacillus and Prochloraz, add can join after the insecticides adjuvant wetting powder, aqueous suspension agent or aqueous emulsion.After in active component bacillus and Prochloraz, adding auxiliary agent and regulating pH value 5~7, be made into wetting powder.Prochloraz can adopt former medicine, and bacillus subtilis or Bacilluscereus, can adopt former powder.Insecticides adjuvant can be selected in wetting agent, dispersant, filler, binding agent, the disintegrant etc. one or more for use.
Composition of the present invention has alleviated existing germ respectively to the pesticide resistance of Prochloraz and bacillus, after bacillus subtilis or waxy Bacillus and Prochloraz combination, has the obvious synergistic effect between the two, reduced simultaneously dosage again, thereby make said composition can be widely used in preventing and treating banded sclerotial blight, bakanae disease, rice blast or the false smut etc. of paddy rice, the downy mildew of tomato, the powdery mildew of cucumber, the powdery mildew of strawberry, gray mold, the crown rot of banana, anthracnose, the blossom-end rot of oranges and tangerines, anthracnose, penicilliosis, green mould etc.
Embodiment
Example of formulations 1:31% wetting powder (each component is a weight portion)
Bacillus subtilis (5,000,000,000 spore alive/gram) 30
Prochloraz 1
Lauryl sodium sulfate 5
Penetrating agent JFC 4
White carbon complements to 100 (adjust pHs 5~7).
Example of formulations 2:20% wetting powder (each component is a weight portion)
Bacilluscereus, (24,000,000,000 spore alive/gram) 10
Prochloraz 10
Emulsifier op-10 6
Dispersing agent NNO 4
Lactose 10
Starch complements to 100 (adjust pHs 5~7).
Example of formulations 3:60% wetting powder (each component is a weight portion)
Bacillus subtilis (20,000,000,000 spore alive/gram) 50
Prochloraz 10
Detergent LS 2
Diffusion agent N 4
Lauryl sodium sulfate 2
Precipitated calcium carbonate 32 (adjust pH 5~7).
Example of formulations 4:80% wetting powder (each component is a weight portion)
Bacilluscereus, (1,000 hundred million spore alive/gram) 20
Prochloraz 60
Draw back powder 2
Bay alcohol radical sodium sulphate 3
Lignin 4
White carbon 11 (adjust pH 5~7).
Drug effect embodiment 1
Bacillus subtilis (24,000,000,000 live in spore/gram) and Prochloraz are made into 20% wetting powder in the ratio of 1: 1 (mass ratio), spraying, be applied to treat bakanae disease of rice, separately bacillus subtilis and Prochloraz be made into above-mentioned wetting powder and contrast, the results are shown in Table 1.
Table 1
Figure A20091003329900061
Drug effect embodiment 2
Bacillus subtilis (5,000,000,000 live in spore/gram) and Prochloraz are made into 31% wetting powder in the ratio of 30: 1 (mass ratio), spraying, be applied to treat tomato downy mildew, separately bacillus subtilis and Prochloraz be made into above-mentioned wetting powder and contrast, the results are shown in Table 2.
Table 2
Figure A20091003329900062
Drug effect embodiment 3
Bacillus subtilis (50,000,000,000 live in spore/gram) and Prochloraz are made into 70% wetting powder in the ratio of 1: 30 (mass ratio), spraying, be applied to treat powdery mildew of cucumber, separately bacillus subtilis and Prochloraz be made into above-mentioned wetting powder and contrast, the results are shown in Table 3.
Table 3
Figure A20091003329900063
Drug effect embodiment 4
Bacillus subtilis (20,000,000,000 live in spore/gram) and Prochloraz are made into 44% wetting powder in the ratio of 10: 1 (mass ratio), spraying, be applied to treat the banana crown rot, separately bacillus subtilis and Prochloraz be made into above-mentioned wetting powder and contrast, the results are shown in Table 4.
Table 4
Drug effect embodiment 5
Bacillus subtilis (1,000 hundred million live in spore/gram) and Prochloraz are made into 20% wetting powder in the ratio of 1: 100 (mass ratio), spraying, be applied to treat the oranges and tangerines blossom-end rot, separately bacillus subtilis and Prochloraz be made into above-mentioned wetting powder and contrast, the results are shown in Table 3.
Table 3

Claims (10)

1, a kind of composition that contains bacillus subtilis or Bacilluscereus, and Prochloraz is characterized in that said composition is an active component with bacillus and Prochloraz, and its weight ratio is 10000: 1~1: 10000; Wherein said bacillus is bacillus subtilis or Bacilluscereus.
2, composition according to claim 1, the weight ratio that it is characterized in that bacillus and Prochloraz is 1000: 1~1: 1000.
3, composition according to claim 2, the weight ratio that it is characterized in that spore bacillus and Prochloraz is 100: 1~1: 100.
4, composition according to claim 2, the weight ratio that it is characterized in that spore bacillus and Prochloraz is 10: 1~1: 100.
5, composition according to claim 2, the weight ratio that it is characterized in that spore bacillus and Prochloraz is 10: 1~1: 10.
6, according to claim 1,2 or 3 described compositions, the spore count that it is characterized in that living in the described bacillus is 0.1 hundred million/gram~1,000 hundred million/gram.
7, composition according to claim 4, the spore count that it is characterized in that living in the described bacillus is 0.5 hundred million/gram~500 hundred million/gram.
8, composition according to claim 5, the spore count that it is characterized in that living in the described bacillus is 100,000,000/gram~100 hundred million/gram.
9, according to claim 1,2 or 3 described compositions, it is characterized in that in active component bacillus and Prochloraz adding join behind the auxiliary agent wetting powder, aqueous suspension agent or aqueous emulsion.
10, composition according to claim 7 after it is characterized in that adding auxiliary agent in active component bacillus and Prochloraz and regulating pH value 5~7, is made into wetting powder.
CN200910033299XA 2009-06-15 2009-06-15 Composition containing bacillus subtilis or waxy bacillus and prochloraz Expired - Fee Related CN101574090B (en)

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102329758A (en) * 2011-10-12 2012-01-25 淮阴师范学院 Biological prevention bacterial strain BS1 (bacillus cereus) for preventing and controlling greenhouse cucumber downy mildew and application thereof
CN102399712A (en) * 2011-09-06 2012-04-04 南京农大生物源农药创制有限公司 Bacterial strain CHS55 for controlling cucumber powdery mildew and downy mildew
CN101962623B (en) * 2010-01-19 2012-05-30 四川农业大学 Bacillus stain for preventing and controlling rice blast and rice sheath blight
CN102533610A (en) * 2012-02-09 2012-07-04 河北省农林科学院植物保护研究所 Bacillus subtilis for preventing and treating cucumber ampho disease, and microbial agent thereof
WO2013178648A1 (en) * 2012-05-30 2013-12-05 Bayer Cropscience Ag Composition comprising a biological control agent and a fungicide selected from inhibitors of the ergosterol biosynthesis
US9364006B2 (en) 2012-05-30 2016-06-14 Bayer Cropscience Ag Composition comprising a biological control agent and a fungicide
CN106818130A (en) * 2016-12-29 2017-06-13 广西桂人堂金花茶产业集团股份有限公司 A kind of method for improving the Camellia nitidissima disease and insect resistance cured matter of increase
CN106986378A (en) * 2017-04-15 2017-07-28 柏玉兰 Panama disease microbe soil restoration agent can be prevented and treated
CN107087640A (en) * 2017-05-19 2017-08-25 安徽省农业科学院植物保护与农产品质量安全研究所 A kind of bactericidal composition and its application containing bacillus amyloliquefaciens and Prochloraz
CN107974427A (en) * 2018-01-23 2018-05-01 浙江省柑桔研究所 One plant of marine streptomyces with bacteriostatic activity
CN108570442A (en) * 2018-07-24 2018-09-25 湖北省农业科学院经济作物研究所 A kind of rapid induction production spore method of anthrax-bacilus
CN111543438A (en) * 2019-01-23 2020-08-18 安徽省农业科学院蚕桑研究所 Composition for preventing and treating mulberry sclerotinia and prevention and treatment method

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* Cited by examiner, † Cited by third party
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CN100351371C (en) * 2005-04-28 2007-11-28 武汉绿世纪生物工程有限责任公司 Antiseptic antistaling agent containing hay bacillocin

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101962623B (en) * 2010-01-19 2012-05-30 四川农业大学 Bacillus stain for preventing and controlling rice blast and rice sheath blight
CN102399712A (en) * 2011-09-06 2012-04-04 南京农大生物源农药创制有限公司 Bacterial strain CHS55 for controlling cucumber powdery mildew and downy mildew
CN102329758B (en) * 2011-10-12 2012-10-03 淮阴师范学院 Biological prevention bacterial strain BS1 (bacillus cereus) for preventing and controlling greenhouse cucumber downy mildew and application thereof
CN102329758A (en) * 2011-10-12 2012-01-25 淮阴师范学院 Biological prevention bacterial strain BS1 (bacillus cereus) for preventing and controlling greenhouse cucumber downy mildew and application thereof
CN102533610A (en) * 2012-02-09 2012-07-04 河北省农林科学院植物保护研究所 Bacillus subtilis for preventing and treating cucumber ampho disease, and microbial agent thereof
CN102533610B (en) * 2012-02-09 2013-06-19 河北省农林科学院植物保护研究所 Bacillus subtilis for preventing and treating cucumber ampho disease, and microbial agent thereof
US9700056B2 (en) 2012-05-30 2017-07-11 Bayer Cropscience Ag Composition comprising a biological control agent and a fungicide selected from inhibitors of the ergosterol biosynthesis
WO2013178648A1 (en) * 2012-05-30 2013-12-05 Bayer Cropscience Ag Composition comprising a biological control agent and a fungicide selected from inhibitors of the ergosterol biosynthesis
US9364006B2 (en) 2012-05-30 2016-06-14 Bayer Cropscience Ag Composition comprising a biological control agent and a fungicide
CN106818130A (en) * 2016-12-29 2017-06-13 广西桂人堂金花茶产业集团股份有限公司 A kind of method for improving the Camellia nitidissima disease and insect resistance cured matter of increase
CN106986378A (en) * 2017-04-15 2017-07-28 柏玉兰 Panama disease microbe soil restoration agent can be prevented and treated
CN107087640A (en) * 2017-05-19 2017-08-25 安徽省农业科学院植物保护与农产品质量安全研究所 A kind of bactericidal composition and its application containing bacillus amyloliquefaciens and Prochloraz
CN107087640B (en) * 2017-05-19 2020-07-21 安徽省农业科学院植物保护与农产品质量安全研究所 Bactericidal composition containing bacillus amyloliquefaciens and prochloraz and application thereof
CN107974427A (en) * 2018-01-23 2018-05-01 浙江省柑桔研究所 One plant of marine streptomyces with bacteriostatic activity
CN107974427B (en) * 2018-01-23 2020-06-19 浙江省柑桔研究所 Marine streptomyces with bacteriostatic activity
CN108570442A (en) * 2018-07-24 2018-09-25 湖北省农业科学院经济作物研究所 A kind of rapid induction production spore method of anthrax-bacilus
CN111543438A (en) * 2019-01-23 2020-08-18 安徽省农业科学院蚕桑研究所 Composition for preventing and treating mulberry sclerotinia and prevention and treatment method

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