CN114946887A - Preparation method and application of environment-friendly seed coating agent - Google Patents
Preparation method and application of environment-friendly seed coating agent Download PDFInfo
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- CN114946887A CN114946887A CN202210620732.5A CN202210620732A CN114946887A CN 114946887 A CN114946887 A CN 114946887A CN 202210620732 A CN202210620732 A CN 202210620732A CN 114946887 A CN114946887 A CN 114946887A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/38—Trichoderma
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
Abstract
The invention provides an environment-friendly seed coating agent, which comprises the following components in percentage by weight: 30-80% of microbial agent fermentation liquor, 1-8% of plant growth regulator, 1-6% of dispersant, 2-6% of film-forming agent, 0.1-2% of preservative, 0.5-3% of thickening agent, 0.1-2% of antifreezing agent, 0.1-1.5% of warning agent and water, and the balance being 100%; the microbial agent is one or two of trichoderma harzianum fermentation liquor, bacillus amyloliquefaciens fermentation liquor, bacillus subtilis fermentation liquor or bacillus licheniformis fermentation liquor; the plant growth regulator comprises: three or more components of humic acid, alginic acid, S-abscisic acid or chitosan. The seed coating agent is used for coating seeds, and can effectively prevent and control wheat sharp eyespot or corn head smut.
Description
Technical Field
The invention relates to the technical field of pesticides, and particularly relates to a preparation method and application of an environment-friendly seed coating agent.
Background
The seeds are the basis of agricultural planting production, and the sowing quality of the seeds is an important factor related to the agricultural production efficiency, so that the research on the seed protection technology is essential. The problem that the germination of seeds is easily affected by pathogenic bacteria carried by the seeds or plant diseases and insect pests in soil needs to be solved, and excessive putting of pesticides and chemical fertilizers in production can cause a large amount of accumulation, so that the problem of environmental pollution can be caused and the activity of the seeds can be affected. Therefore, the seeds are effectively treated in the early stage of sowing so as to control the occurrence of plant diseases and insect pests and improve the germination condition of the seeds and the growth condition of the seeds in the seedling period, thereby ensuring the yield of crops, solving the problem of excessive application, saving the cost and improving the economic benefit, and being a necessary way for agricultural modernization.
The seed coating is a process of treating seeds with a medicament having film-forming properties to form a film having a certain function on the surface of the seeds. The agents used for coating seeds are called seed coatings. The seed coating agent is generally prepared by mixing and processing active substances (pesticide stock solution, biological agent and the like), a dispersing agent, a film forming agent, a binding agent, a dispersing agent, a preservative, warning color and other components. Used directly or diluted for seed treatment. After the seeds are coated and sown, a protective barrier can be formed around the seeds, and the damage of the seeds by plant diseases and insect pests in soil is reduced. The coated seeds absorb water in the soil and then swell slightly, but the coating agent does not dissolve. The effective components of the seed coating agent are gradually absorbed by the plant root system in the seed germination and growth processes, are conducted to the parts of the plant such as stems, leaves and the like through the inside of plant tissues, and simultaneously have the effect of improving the microenvironment of root soil, so that the resistance of the plant to pest and disease infection in the germination stage and the seedling stage is enhanced, and simultaneously, the seed coating agent has the effects of promoting the plant growth and increasing the yield of crops. The seed coating technology has the advantage of simple and convenient operation and is widely applied to preventing and controlling crop seedling diseases and insect pests.
The biological seed coating agent is an environment-friendly preparation, and the active ingredients of the biological seed coating agent mainly take substances with antagonistic microbe effects, such as natural products, microbe living bodies, microbe metabolites and the like as main bodies, and then the biological seed coating agent is processed by adding an auxiliary agent. In the biological seed coating agent, the active substance generally has longer survival time and unchanged activity, and effectively controls plant diseases and insect pests through antagonism or competition mechanism with the diseases and insect pests. Compared with the chemical seed coating agent, the biological seed coating agent has the following advantages: firstly, after the biological seed coating agent is applied, the microenvironment of root soil can be improved, and the fusion degree of seeds and soil is improved; secondly, most of the biological seed coating agents have lower production cost and better effect; thirdly, the long-term use of chemical agents can cause the problem of '3R', and the biological seed coating agent can avoid the problems; fourthly, the biological seed coating agent is safe to people and livestock, is environment-friendly, can be applied for a long time, and is not easy to cause the problem of drug resistance of crops. The biological pesticide has the advantages of high safety to human, livestock and environment, no residue and the like, and is a hot problem in current research.
Shanghai university of transportation (CN106386869A) discloses a trichoderma seed coating agent, which comprises trichoderma chlamydospore fermentation liquor, a film-forming agent, chitosan oligosaccharide, shenqinmycin and humic acid; wherein, each liter of trichoderma chlamydospore fermentation liquor contains 13 to 17 percent of film forming agent, 0.35 to 0.45 percent of chitosan oligosaccharide, 0.5 to 1.5ug/ml of shenqinmycin and 0.05 to 0.1 percent of humic acid, and the percentages are mass-volume ratio; the formula contains bactericidal shenqinmycin besides a microbial inoculum, and the growth regulator is used singly and cannot play a good role in preventing and treating diseases. The compound microorganism rice seed coating agent is prepared from 2-30% of bacillus subtilis, 2-30% of brevibacillus laterosporus, 0.1-15% of trichoderma harzianum, 1-30% of a film-forming agent, 1-10% of a suspending agent, 1-16% of a suspending agent, 1-30% of a quick curing agent, 1-5% of fulvic acid and 20-40% of water by weight percentage; the formula only contains a biological microbial inoculum and does not contain components for regulating plant growth, and the control effect is poor.
Disclosure of Invention
The invention aims to provide a preparation method and application of an environment-friendly seed coating agent, and aims to solve the technical problems of increased disease resistance, environmental pollution and the like of chemical agents in the prior art.
In order to solve the technical problems, the invention provides the following technical scheme:
an environment-friendly seed coating agent comprises the following components in percentage by weight: 30-80% of microbial agent fermentation liquor, 1-8% of plant growth regulator, 1-6% of dispersant, 2-6% of film-forming agent, 0.1-2% of preservative, 0.5-3% of thickening agent, 0.1-2% of antifreezing agent, 0.1-1.5% of warning agent and 100% of water;
the microbial agent is one or two of trichoderma harzianum fermentation liquor, bacillus amyloliquefaciens fermentation liquor, bacillus subtilis fermentation liquor or bacillus licheniformis fermentation liquor; the microbial inoculum can be any existing bacterial strain; preferably, the following components are used: trichoderma harzianum LTR-2 (source: China general microbiological culture Collection center, preservation number: CGMCC NO. 1498); bacillus amyloliquefaciens EA19 (the source is China center for type culture Collection, the preservation number is CCTCC NO. M209316); bacillus subtilis A16 (source: China center for type culture Collection, preservation number is CCTCC NO: M209105); bacillus licheniformis 202 (source: China center for type culture Collection, preservation number is CCTCC NO: M207093). The concentration of the fermentation liquor is 10 8 -10 11 cfu/ml。
The plant growth regulator comprises: three or more components of humic acid, alginic acid, S-abscisic acid or chitosan. Preferably, the plant growth regulator comprises humic acid, alginic acid, S-abscisic acid and chitosan; and the mass ratio of the humic acid to the alginic acid to the S-abscisic acid to the chitosan is 1-3:1-3:1-3: 1-3.
Preferably, the dispersant is one or more of sodium dodecyl benzene sulfonate, calcium dodecyl benzene sulfonate, sodium dodecyl sulfate or sodium dodecyl sulfonate; the film forming agent is one or more of sodium carboxymethylcellulose, hydroxypropyl methylcellulose, ethyl cellulose or hydroxypropyl cellulose; the preservative is one or more of potassium sorbate, sodium benzoate, sodium acetate dehydrate or isobutyl paraben; the thickening agent is one or more than two of xanthan gum, attapulgite, cationic guar gum or polyvinyl alcohol; the antifreezing agent is propylene glycol; the warning agent is one or two of iron oxide red, red powder or acid scarlet.
The invention relates to application of an environment-friendly seed coating agent in seed coating. Preferably, the seeds are wheat seeds or corn seeds; especially for preventing and controlling wheat sharp eyespot or corn head smut.
Compared with the prior art, the invention has the following beneficial technical effects:
when the trichoderma harzianum fermentation liquor is combined with three or more than three components of plant growth regulator humic acid, alginic acid, S-abscisic acid or chitosan for use, the control effect of the seed coating agent on wheat sharp eyespot and corn head smut can be obviously improved. The combination of the trichoderma harzianum fermentation liquor and the bacillus subtilis fermentation liquor can improve the control effect of the seed coating agent on wheat sharp eyespot and maize head smut
Detailed Description
The present invention will be further explained with reference to specific embodiments.
Example 1 seed coating formulation of a Single inoculant formulation
Preparation 1: an environment-friendly seed coating agent comprises the following components in percentage by weight: 75% of microbial agent fermentation liquor, 2% of plant growth regulator, 2% of dispersant, 5% of film-forming agent, 0.5% of preservative, 1.5% of thickening agent, 1% of antifreezing agent, 0.5% of warning agent and water which are added to make up to 100%;
the microbial agent is trichoderma harzianum fermentationLiquid; at a concentration of 10 9 cfu/ml；
The plant growth regulator comprises: humic acid, alginic acid, S-abscisic acid and chitosan; the mass ratio of the humic acid to the alginic acid to the S-abscisic acid to the chitosan is 1:1:1: 1;
the dispersant is sodium dodecyl benzene sulfonate;
the film-forming agent is sodium carboxymethyl cellulose;
the preservative is potassium sorbate;
the thickening agent is xanthan gum;
the antifreezing agent is propylene glycol;
the warning agent is acid scarlet
The preparation method comprises the following steps: weighing the raw materials, firstly putting the film forming agent and the thickening agent into a container, adding a certain amount of sterile water, carrying out boiling water bath until the film forming agent and the thickening agent are dissolved to be sticky, and cooling to 40-50 ℃; then adding other raw materials except the microbial inoculum, and mixing and stirring uniformly. And finally, adding the Hatz raspberry fermentation liquor, and fully mixing and stirring uniformly.
Preparing a trichoderma harzianum fermentation liquid: the corn powder is prepared by adopting corn powder culture solution (the corn powder culture solution is 50g of corn powder, 0.5g of ammonium sulfate and 10g of cane sugar, and the corn powder culture solution is added to a constant volume of 1L and the pH value is adjusted to 5-7) for liquid fermentation, 0.5 percent of fermentation strain is inoculated into a liquid culture medium, the fermentation temperature is 28 +/-1 ℃, and the fermentation time is 7-8 days.
Preparation 2: an environment-friendly seed coating agent comprises the following components in percentage by weight: 60% of microbial agent fermentation liquor, 4% of plant growth regulator, 3% of dispersant, 3% of film-forming agent, 0.8% of preservative, 2.0% of thickening agent, 1.5% of antifreezing agent, 0.5% of warning agent and the balance of water to 100%;
the microbial agent is trichoderma harzianum fermentation liquor; at a concentration of 10 9 cfu/ml；
The plant growth regulator comprises: humic acid, alginic acid, S-abscisic acid and chitosan; the mass ratio of the humic acid to the alginic acid to the S-abscisic acid to the chitosan is 1:3:1: 2;
the dispersant is sodium dodecyl sulfate;
the film forming agent is hydroxypropyl cellulose;
the preservative is sodium benzoate;
the thickening agent is xanthan gum;
the antifreezing agent is propylene glycol;
the warning agent is acid scarlet
The preparation method comprises the following steps: the same as preparation 1.
Preparation 3: an environment-friendly seed coating agent comprises the following components in percentage by weight: 40% of microbial agent fermentation liquor, 6% of plant growth regulator, 4% of dispersant, 4% of film-forming agent, 1.0% of preservative, 1.0% of thickening agent, 2% of antifreezing agent, 0.5% of warning agent and the balance of water to 100%;
the microbial agent is trichoderma harzianum fermentation liquor; at a concentration of 10 9 cfu/ml；
The plant growth regulator comprises: humic acid, alginic acid, S-abscisic acid and chitosan; the mass ratio of the humic acid to the alginic acid to the S-abscisic acid to the chitosan is 2:2:1: 1;
the dispersant is sodium dodecyl benzene sulfonate;
the film-forming agent is sodium carboxymethyl cellulose;
the preservative is potassium sorbate;
the thickening agent is xanthan gum;
the antifreezing agent is propylene glycol;
the warning agent is acid scarlet
The preparation method comprises the following steps: the same as preparation 1.
Preparation 4: an environment-friendly seed coating agent comprises the following components in percentage by weight: 75% of microbial agent fermentation liquor, 2% of plant growth regulator, 2% of dispersing agent, 5% of film-forming agent, 0.5% of preservative, 1.5% of thickening agent, 1% of antifreezing agent, 0.5% of warning agent and water, wherein the amount of water is up to 100%;
the microbial agent is trichoderma harzianum fermentation liquor; at a concentration of 10 9 cfu/ml；
The plant growth regulator comprises: humic acid, alginic acid and S-abscisic acid; the mass ratio of the humic acid to the alginic acid to the S-abscisic acid is 1:1: 1;
the dispersant is sodium dodecyl benzene sulfonate;
the film forming agent is ethyl cellulose;
the preservative is potassium sorbate;
the thickening agent is xanthan gum;
the antifreezing agent is propylene glycol;
the warning agent is acid scarlet
The preparation method comprises the following steps: the same as preparation 1.
Preparation 5: an environment-friendly seed coating agent comprises the following components in percentage by weight: 75% of microbial agent fermentation liquor, 2% of plant growth regulator, 2% of dispersant, 5% of film-forming agent, 0.5% of preservative, 1.5% of thickening agent, 1% of antifreezing agent, 0.5% of warning agent and water which are added to make up to 100%;
the microbial agent is trichoderma harzianum fermentation liquor; at a concentration of 10 9 cfu/ml；
The plant growth regulator comprises: humic acid, alginic acid and chitosan; the mass ratio of the humic acid to the alginic acid to the chitosan is 1:1: 1;
the dispersant is sodium dodecyl benzene sulfonate;
the film-forming agent is sodium carboxymethyl cellulose;
the preservative is potassium sorbate;
the thickening agent is xanthan gum;
the antifreezing agent is propylene glycol;
the warning agent is acid scarlet
The preparation method comprises the following steps: the same as preparation 1.
Preparation 6: an environment-friendly seed coating agent comprises the following components in percentage by weight: 75% of microbial agent fermentation liquor, 2% of plant growth regulator, 2% of dispersant, 5% of film-forming agent, 0.5% of preservative, 1.5% of thickening agent, 1% of antifreezing agent, 0.5% of warning agent and water which are added to make up to 100%;
the microbial agent is trichoderma harzianum fermentation liquor; at a concentration of10 9 cfu/ml；
The plant growth regulator comprises: alginic acid, S-abscisic acid and chitosan; the mass ratio of the alginic acid to the S-abscisic acid to the chitosan is 1:1: 1;
the dispersant is sodium dodecyl benzene sulfonate;
the film-forming agent is sodium carboxymethyl cellulose;
the preservative is potassium sorbate;
the thickening agent is xanthan gum;
the antifreezing agent is propylene glycol;
the warning agent is acid scarlet
The preparation method comprises the following steps: the same as preparation 1.
Preparation 7: an environment-friendly seed coating agent comprises the following components in percentage by weight: 75% of microbial agent fermentation liquor, 2% of plant growth regulator, 2% of dispersant, 5% of film-forming agent, 0.5% of preservative, 1.5% of thickening agent, 1% of antifreezing agent, 0.5% of warning agent and water which are added to make up to 100%;
the microbial agent is trichoderma harzianum fermentation liquor; at a concentration of 10 9 cfu/ml；
The plant growth regulator comprises: humic acid and alginic acid; the mass ratio of the humic acid to the alginic acid is 1: 1;
the dispersant is sodium dodecyl benzene sulfonate;
the film-forming agent is sodium carboxymethyl cellulose;
the preservative is potassium sorbate;
the thickening agent is xanthan gum;
the antifreezing agent is propylene glycol;
the warning agent is acid scarlet
The preparation method comprises the following steps: same as preparation 1.
Preparation 8: an environment-friendly seed coating agent comprises the following components in percentage by weight: 75% of microbial agent fermentation liquor, 2% of plant growth regulator, 2% of dispersant, 5% of film-forming agent, 0.5% of preservative, 1.5% of thickening agent, 1% of antifreezing agent, 0.5% of warning agent and water which are added to make up to 100%;
the microbial agent is trichoderma harzianum fermentation liquor; at a concentration of 10 9 cfu/ml；
The plant growth regulator comprises: humic acid and chitosan; the mass ratio of the humic acid to the chitosan is 1: 1;
the dispersant is sodium dodecyl benzene sulfonate;
the film-forming agent is sodium carboxymethyl cellulose;
the preservative is potassium sorbate;
the thickening agent is xanthan gum;
the antifreezing agent is propylene glycol;
the warning agent is acid scarlet
The preparation method comprises the following steps: the same as preparation 1.
Preparation 9: an environment-friendly seed coating agent comprises the following components in percentage by weight: 75% of microbial agent fermentation liquor, 2% of plant growth regulator, 2% of dispersant, 5% of film-forming agent, 0.5% of preservative, 1.5% of thickening agent, 1% of antifreezing agent, 0.5% of warning agent and water which are added to make up to 100%;
the microbial agent is trichoderma harzianum fermentation liquor; at a concentration of 10 9 cfu/ml；
The plant growth regulator comprises: alginic acid and chitosan; the mass ratio of the alginic acid to the chitosan is 1: 1;
the dispersant is sodium dodecyl benzene sulfonate;
the film-forming agent is sodium carboxymethyl cellulose;
the preservative is potassium sorbate;
the thickening agent is xanthan gum;
the antifreezing agent is propylene glycol;
the warning agent is acid scarlet
The preparation method comprises the following steps: the same as preparation 1.
Preparation 10: an environment-friendly seed coating agent comprises the following components in percentage by weight: 75% of microbial agent fermentation liquor, 2% of dispersing agent, 5% of film-forming agent, 0.5% of preservative, 1.5% of thickening agent, 1% of antifreezing agent, 0.5% of warning agent and water, wherein the amount of water is up to 100%;
the microbial agent is trichoderma harzianum fermentation liquor; at a concentration of 10 9 cfu/ml；
The dispersant is sodium dodecyl benzene sulfonate;
the film-forming agent is sodium carboxymethyl cellulose;
the preservative is potassium sorbate;
the thickening agent is xanthan gum;
the antifreezing agent is propylene glycol;
the warning agent is acid scarlet
The preparation method comprises the following steps: the same as preparation 1.
Example 2 seed coating formulation of Complex microbial inoculum
Preparation 11: an environment-friendly seed coating agent comprises the following components in percentage by weight: 60% of microbial agent fermentation liquor, 3% of plant growth regulator, 3.5% of dispersant, 4% of film-forming agent, 1.0% of preservative, 2.0% of thickening agent, 1.0% of antifreezing agent, 0.5% of warning agent and water, wherein the balance is 100%;
the microbial agent is trichoderma harzianum fermentation liquor and bacillus amyloliquefaciens fermentation liquor; the volume ratio of the two is 1: 1; the concentration of the Trichoderma harzianum fermentation liquor is 10 9 cfu/ml; the concentration of the bacillus amyloliquefaciens fermentation liquor is 10 8 cfu/ml。
The plant growth regulator comprises: humic acid, alginic acid, S-abscisic acid and chitosan; the mass ratio of the humic acid to the alginic acid to the S-abscisic acid to the chitosan is 1:1:1: 1;
the dispersant is calcium dodecyl benzene sulfonate;
the film-forming agent is sodium carboxymethyl cellulose;
the preservative is potassium sorbate;
the thickening agent is xanthan gum;
the antifreezing agent is propylene glycol;
the warning agent is acid bright red.
The preparation method comprises the following steps: the same as preparation 1.
Preparing a bacillus amyloliquefaciens fermentation liquid: inoculating Bacillus amyloliquefaciens in culture solution (10 g/L glucose, 14g/L corn flour, 16g/L soybean cake powder, and water in balance), and fermenting.
Preparation 12: an environment-friendly seed coating agent comprises the following components in percentage by weight: 60% of microbial agent fermentation liquor, 4% of plant growth regulator, 4% of dispersant, 4% of film-forming agent, 0.5% of preservative, 1.0% of thickening agent, 1.0% of antifreezing agent, 0.5% of warning agent and the balance of water to 100%;
the microbial agent is trichoderma harzianum fermentation liquor and bacillus subtilis fermentation liquor; the volume ratio of the two is 4: 1; the concentration of the Trichoderma harzianum fermentation liquor is 10 9 cfu/ml; the concentration of the bacillus subtilis fermentation liquor is 10 9 cfu/ml。
The plant growth regulator comprises: humic acid, alginic acid, S-abscisic acid and chitosan; the mass ratio of the humic acid to the alginic acid to the S-abscisic acid to the chitosan is 1:1:1: 1;
the dispersant is calcium dodecyl benzene sulfonate;
the film-forming agent is sodium carboxymethyl cellulose;
the preservative is potassium sorbate;
the thickening agent is xanthan gum;
the antifreezing agent is propylene glycol;
the warning agent is acid bright red.
The preparation method comprises the following steps: the same as preparation 1.
Preparing a bacillus subtilis fermentation liquid: inoculating the bacillus subtilis to an LB culture medium, and performing shaking culture and fermentation on the bacillus subtilis in a shaking table to obtain bacillus subtilis fermentation liquor.
Preparation 13: an environment-friendly seed coating agent comprises the following components in percentage by weight: 60% of microbial agent fermentation liquor, 4% of plant growth regulator, 2% of dispersant, 2% of film-forming agent, 0.5% of preservative, 1.5% of thickening agent, 1.5% of antifreezing agent, 0.5% of warning agent and the balance of water to 100%;
the microbial agent is trichoderma harzianum fermentation liquor and bacillus licheniformis fermentation liquor; both bodiesThe product ratio is 1: 2; the concentration of the Trichoderma harzianum fermentation liquor is 10 8 cfu/ml; the concentration of the Bacillus licheniformis fermentation liquor is 10 9 cfu/ml。
The plant growth regulator comprises: humic acid, alginic acid, S-abscisic acid and chitosan; the mass ratio of the humic acid to the alginic acid to the S-abscisic acid to the chitosan is 1:1:1: 1;
the dispersant is calcium dodecyl benzene sulfonate;
the film-forming agent is sodium carboxymethyl cellulose;
the preservative is potassium sorbate;
the thickening agent is xanthan gum;
the antifreezing agent is propylene glycol;
the warning agent is acid bright red.
The preparation method comprises the following steps: the same as preparation 1.
Preparing a bacillus licheniformis fermentation liquid: the bacillus licheniformis is inoculated in a liquid culture medium (2 percent of corn flour, 1.0 percent of peptone, 0.5 percent of yeast powder, 2.0 percent of sucrose, 2.0 percent of sodium chloride, 0.02 percent of magnesium sulfate and 0.05 percent of manganese sulfate) for fermentation preparation.
Example 3: control effect of different seed coating agents on wheat sharp eyespot
And (3) testing crop diseases: sheath blight of wheat
The test method comprises the following steps: reference GB/T17980.108-2004 pesticide field test guidelines (second) part 108: the bactericide can be used for preventing and treating wheat sharp eyespot. The field plot experiment is adopted, the plot area is 50m2, the arrangement is carried out by blocks at any time, and the operation is repeated for four times. Soaking seeds before sowing the wheat (seed coating agent is diluted by 100 times: 1L seed coating agent, water is added for 99L, the seeds are placed in the diluent to be stirred and soaked for 4h, and the seeds can be sowed after being fished out and dried); disease investigation is carried out in the jointing stage, five points of diagonal lines of each cell are sampled, 100 plants are investigated at each point, and disease grading is carried out:
level 0: the disease is not developed;
level 1: the leaf sheath is diseased but the stem is not diseased;
and 3, level: the leaf sheath is diseased and invades the stem, but the stalk lesion has insufficient ring stem 1/2;
and 5, stage: the stigma canephora exceeds 1/2, but does not fall or break;
and 7, stage: withering, lodging and white ear.
The disease index [ ∑ (number of diseased plants at each level × relative level value)/(number of total investigated plants × 7] × 100;
the prevention and treatment effect is [ (control disease index-treatment disease index)/control disease index ] × 100%.
And (3) testing results: as can be seen from Table 1, when the Trichoderma harzianum fermentation broth is used in combination with three or more components of plant growth regulators such as humic acid, alginic acid, S-abscisic acid or chitosan, the control effect of the seed coating agent on wheat sharp eyespot can be remarkably improved. In formulas 1-3, when the trichoderma harzianum fermentation broth and the plant growth regulator (humic acid, alginic acid, S-abscisic acid and chitosan) are used in combination, the control effects on wheat sharp eyespot are 90.2%, 85.5% and 81.6% respectively. Compared with the formula 4-10, the formula 1 has the advantage that the composition of the plant growth regulator has obvious influence on the control effect of the wheat sharp eyespot. In addition, the combination of the trichoderma harzianum fermentation liquor and the bacillus subtilis fermentation liquor can improve the control effect of the seed coating agent on wheat sharp eyespot.
Table 1: field control effect of different seed coating agents on wheat sharp eyespot
Example 3 prevention and treatment Effect of different seed coating agents on maize head smut
And (3) testing crop diseases: head smut of maize
The test method comprises the following steps: reference GB/T17980.106-2004 PestIcIde field test guidelines (second) part 106: the bactericide can be used for preventing and treating maize head smut. The field plot experiment is adopted, the plot area is 60m2, the arrangement is carried out by blocks at any time, and the process is repeated for four times. Soaking seeds before sowing the corns (the seed coating agent is diluted by 150 times and added with 149L of water in 1L of the seed coating agent; the seeds are put into the diluent to be stirred and soaked for 6 hours, and the seeds can be sowed after being fished out and dried); disease investigation is carried out when symptoms are obvious after the fruit clusters are full, investigation is carried out on all the plants except the side rows in each cell, the total number of plants and the number of diseased plants are recorded, and the disease rate and the control effect are calculated
The disease rate is the number of diseased plants/the total number of investigated plants x 100%;
the control effect is [ (control plant disease rate-treatment plant disease rate)/control plant disease rate ] × 100%.
And (3) testing results: as can be seen from Table 2, when the Trichoderma harzianum fermentation broth is used in combination with three or more of humic acid, alginic acid, S-abscisic acid or chitosan, the prevention and treatment effect of the seed coating agent on the maize head smut can be significantly improved. In formulas 1-3, when the trichoderma harzianum fermentation broth and the plant growth regulator combination (humic acid, alginic acid, S-abscisic acid and chitosan) are used in combination, the control effects on the maize head smut are 89.2%, 86.0% and 84.6% respectively. Compared with the formula 4-10, the formula 1 has the advantage that the composition of the plant growth regulator has obvious influence on the prevention and treatment effect of the maize head smut. In addition, the combination of the trichoderma harzianum fermentation liquor and the bacillus subtilis fermentation liquor can improve the control effect of the seed coating agent on the maize head smut.
Table 2: field control effect of different seed coating agents on maize head smut
| Treatment of | Rate of diseased plants | Control effect% |
| Formulation 1 | 3.1 | 89.2 |
| Formulation 2 | 4.0 | 86.0 |
| Formulation 3 | 4.4 | 84.6 |
| Formulation 4 | 8.2 | 71.3 |
| Formulation 5 | 9.2 | 67.8 |
| Formulation 6 | 8.3 | 71.0 |
| Formulation 7 | 10.6 | 62.9 |
| Formulation 8 | 10.8 | 62.2 |
| Formulation 9 | 11.5 | 59.8 |
| Formulation 10 | 13.2 | 53.8 |
| Formulation 11 | 3.3 | 88.5 |
| Formulation 12 | 1.4 | 95.1 |
| Formulation 13 | 2.8 | 90.2 |
| CK | 28.6 | - |
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same.
Claims (7)
1. The environment-friendly seed coating agent is characterized by comprising the following components in percentage by weight: 30-80% of microbial agent fermentation liquor, 1-8% of plant growth regulator, 1-6% of dispersant, 2-6% of film-forming agent, 0.1-2% of preservative, 0.5-3% of thickening agent, 0.1-2% of antifreezing agent, 0.1-1.5% of warning agent and water, and the balance being 100%;
the microbial agent is one or two of trichoderma harzianum fermentation liquor, bacillus amyloliquefaciens fermentation liquor, bacillus subtilis fermentation liquor or bacillus licheniformis fermentation liquor;
the plant growth regulator comprises: three or more components of humic acid, alginic acid, S-abscisic acid or chitosan.
2. The environment-friendly seed coating agent of claim 1, wherein the plant growth regulator comprises humic acid, alginic acid, S-abscisic acid and chitosan; and the mass ratio of the humic acid to the alginic acid to the S-abscisic acid to the chitosan is 1-3:1-3:1-3: 1-3.
3. The environment-friendly seed coating agent as claimed in claim 1 or 2, wherein the dispersant is one or more of sodium dodecyl benzene sulfonate, calcium dodecyl benzene sulfonate, sodium dodecyl sulfate or sodium dodecyl benzene sulfonate; the film forming agent is one or more of sodium carboxymethylcellulose, hydroxypropyl methylcellulose, ethyl cellulose or hydroxypropyl cellulose; the preservative is one or more of potassium sorbate, sodium benzoate, sodium acetate dehydrate or isobutyl paraben; the thickening agent is one or more than two of xanthan gum, attapulgite, cationic guar gum or polyvinyl alcohol; the antifreezing agent is propylene glycol; the warning agent is one or two of iron oxide red, red powder or acid scarlet.
4. The environment-friendly seed coating agent of any one of claims 1 to 3, wherein the concentration of the fermentation liquid is 10 8 -10 11 cfu/ml。
5. Use of the environmentally friendly seed coating according to any one of claims 1 to 4 for coating seeds.
6. Use according to claim 5, wherein the seed is a wheat seed or a corn seed.
7. The use as claimed in claim 5, for the prevention and control of sheath blight of wheat or head smut of maize.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115380777A (en) * | 2022-09-21 | 2022-11-25 | 新疆生产建设兵团第九师农业科学研究所(畜牧科学研究所) | High-yield cultivation method for corn |
| CN115968886A (en) * | 2022-12-05 | 2023-04-18 | 西藏诺迪康药业股份有限公司 | Environment-friendly rhodiola crenulata seed coating agent and coating method in Qinghai-Tibet plateau area |
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2022
- 2022-06-02 CN CN202210620732.5A patent/CN114946887A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115380777A (en) * | 2022-09-21 | 2022-11-25 | 新疆生产建设兵团第九师农业科学研究所(畜牧科学研究所) | High-yield cultivation method for corn |
| CN115968886A (en) * | 2022-12-05 | 2023-04-18 | 西藏诺迪康药业股份有限公司 | Environment-friendly rhodiola crenulata seed coating agent and coating method in Qinghai-Tibet plateau area |
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